Life Sciences Commons^™

Insight Into Eleutherodactylus Coqui Resilience And Anuran Hypoxia Tolerance Utilizing Pincho, Randy Ortiz

Theses and Dissertations

The onset of high-throughput RNA-sequencing (RNA-seq) technology allowed research into transcriptomics to accelerate exponentially aided by the rapid advancement of bioinformatic pipelines. The Pincho workflow, my first research endeavor, is a transcriptomic workflow developed to create an avenue of high-quality reconstructions from RNA-seq data. High-quality reconstruction standards entail longer transcripts, more complete transcripts, and more raw data utilization. We have discovered an ideal trio of assemblers between transABySS, rnaSPAdes and TransLiG that would best reconstruct next-generation sequencing data according to these standards. We utilized Pincho to drive two distinct experiments: (1) exploring the genetic basis for the successful invasion of …

Interferon Gamma Signaling In Ovarian Cancer Cells, Bijaya Gaire

Theses and Dissertations

Interferon gamma (IFNγ) is a pleiotropic cytokine that can have, depending on the cellular and molecular context, both anti-tumor and pro-tumorigenic functions. IFNγ expression can be induced in cancer cells in response to radiation therapy or immune checkpoint blockade used in cancer treatment. We have previously shown that IFNγ induces expression of the Bcl3 proto-oncogene in ovarian cancer (OC) cells, resulting in their increased proliferation and migration, but the mechanisms are unknown. Here, I demonstrate that the IFNγ-induced Bcl3 expression is dependent on JAK1 and STAT1 signaling, and on p65 NFkB. Furthermore, my data show that the IFNγ-induced Bcl3 expression …

Cytotoxicity Of Copper (Ii) Octanoate On Mouse Hippocampal Astrocytes, Ali A. Shohatee

Theses and Dissertations

Copper, as an essential trace element, is crucial for various biological processes but has also been utilized as a constituent of various pesticides for decades. Copper (II) octanoate is a pesticide utilized as a fungicide and is approved for use in the production of certified organic foods. Previous studies have implicated copper as an inducer of oxidative stress and activator of the Nrf2-ARE pathway. Nrf2 is essential in regulating the transcription of various genes to alleviate toxicity such as those involved in glutathione synthesis and detoxification. Due to the limited toxicological data, this study into the potential induction of oxidative …

Comparing Insect Biodiversity Between Curb-Cut Rain Gardens And Residential Lawns, Dave Warners, Naomi Dykstra, Julia Klooster

Summer Research

Global insect biodiversity is at a major decline. The drop in population can largely be attributed to a loss of natural habitat through conversion into urban and agricultural land. Insects are important parts of food webs and many play an important role in pollination, control of weeds, control of agricultural pests, and the decomposition of wood and leaves.

Role Of Integrins In Hiv Infection Of Resting Cd4+ T Cells Stimulated By Intestinal Endothelial Cells, Anding Shen, Sarah Logan, Tim Kim, Zihan Zhang

Summer Research

Eradicating HIV remains a formidable challenge due to the persistence of latent reservoirs within resting CD4+ T cells. Intestinal endothelial cells (IECs) have been shown to promote HIV infection and latent reservoir formation in resting CD4+ T cells. Our research investigates the role of integrins and their ligands, specifically ICAM, VCAM, and MAdCAM, in resting T cells and their impact on productive and latent HIV infection. Findings reveal that VCAM stimulation was the most effective in promoting productive HIV infection. MAdCAM and ICAM had minimal effect. Cytokine IL-6 consistently displayed synergy with all CAMs, amplifying effects on productive HIV infection. …

Mechanisms Of Stimulation By Intestinal Endothelial Cells During Hiv Infection Of Activated Cd4+ T Cells, Anding Shen, Timothy Kim, Sarah Logan, Zihan Zhang

Summer Research

Under antiretroviral therapy, the viral persistence of HIV is enabled by latent viral reservoirs, particularly in resting CD4+ T cells. Recent studies reveal interactions between endothelial cells and T cells allowing for latent infection of resting and activated CD4+ T cells. We focused our investigation on the stimulation of activated T cells by intestinal endothelial cells (IECs), due to the prominent gut pathology of HIV and predominance of an activated T cell phenotype in the gut. While prior research shows the involvement of soluble factors like IL-6 in stimulation of resting T cells, our results from transwell cultures suggest the …

Curb Cut Rain Gardens In The Plaster Creek Watershed, Josiah Millen, Cherith Pickett, Brandon Price, Jude Veldboom, Dave Warners

Summer Research

The developed urban life of Grand Rapids, Michigan has flourished in what was once a pure, natural setting. Throughout time, the standardization of impermeable surfaces, the slow takeover of native plants by means of invasive ones, and general increase of pollution has continued onward. This has led to a decrease in the water quality of the Grand Rapids watershed and poses a health concern to the communities immersed in the Plaster Creek Watershed. Plaster Creek Stewards is working to restore the watershed and mitigate further damage focusing on the beneficial impacts of installing curb-cut rain gardens (CCRG).

Selective Reduction Of Nitriles In The Presence Of Aromatic Nitro Groups, Esther Seoha Yoo, Dr. Michael Barbachyn

Summer Research

• Identify reliable aprotic reaction conditions for selective reduction of a nitrile in the presence of a nitro group. •
• Examine a range of nitrobenzonitriles and nitrophenyl acetonitriles to determine the scope of the methodology.

Environmental Surveillance For Blacklegged Ticks (Ixodes Scapularis) Across An Urban-Rural Gradient In West Michigan, William L. Miller, Sophia M. Bieri, An Vu, Audrey Luce, Erica Boldenow, Meghan May, Jean Tsao

Summer Research

The blacklegged tick is an emerging species of ticks (Family: Ixodidae) in Michigan and is the primary vector for Borrelia burgdorferi, the bacterium that causes Lyme disease. The range of the blacklegged tick has increased in the Lower Peninsula of Michigan over the past 20 years, expanding northward along the lakeshore and inland in southern portions of the state. The number of human cases has increased 5-fold over the same timeframe, facilitated by the expansion of blacklegged tick populations.

Anxiety And Depression Among U.S. International Students During The Covid-19 Pandemic, Ismatara Reena, Edward Hebert, Kumer Das, Shankari M. Dipti, Nirmal C. Gope, Raymond Doe

Kinesiology

The COVID-19 pandemic has impacted college students worldwide. International students are a significant group of college students in the United States, making up approximately 5% of enrollment. While research has examined the mental health effects of the COVID-19 pandemic on college students, its impact on international students in the U.S. has not been well-documented. This study examined anxiety and depression levels of 170 international students enrolled in three public U.S. universities. Data were collected using the Generalized Anxiety Disorder (GAD-7) survey and the Patient Health Questionnaire (PHQ-9). Over 60% of the sample reported anxiety and depressive symptoms, with 35.1% showing …

Ecological Risk Assessment For The Western Australian Silverlip Pearl Oyster (Pinctada Maxima) Resource, K. A. Smith, S. Brown, A. M. Hart, A. Bissell

Fisheries Research Reports

In August 2022, the Department of Primary Industries and Regional Development (DPIRD) convened an ecological risk assessment (ERA) of the fishery and aquaculture operations that access the Western Australian (WA) Silverlip Pearl Oyster (Pinctada maxima) resource (Resource).

The ERA considered the potential ecological impacts of the WA commercial Silverlip Pearl Oyster Fishery and related aquaculture activities including hatchery and seeding operations. The assessment evaluated the impact of each activity on all relevant retained and discarded species; threatened, endangered and protected species; habitats and the broader environment. Risks associated with aquaculture activities were also considered, including genetic impacts on …

Resilience Theory And Coerced Resilience In Agriculture, S. M. Sundstrom, D. G. Angeler, C. R. Allen

School of Natural Resources: Documents and Reviews

No abstract provided.

Storms And Ph Of Dam Releases Affects Downstream Phosphorus Cycling In An Arid Regulated River, B. R. Deemer, R. H. Reibold, A. Fatta, J. R. Corman, C. B. Yackulic, S. C. Reed

School of Natural Resources: Documents and Reviews

No abstract provided.

Modified Tracer Gas Injection For Measuring Stream Gas Exchange Velocity In The Presence Of Significant Temperature Variation, C. R. Jensen, D. P. Genereux, T. E. Gilmore, D. K. Solomon

School of Natural Resources: Documents and Reviews

No abstract provided.

Characterization Of Particulate Size Distributions And Water-Soluble Ions Measured At A Broiler Farm, P. J. Silva, T. Cress, R. Drover, C. Michael, G. Docekal, P. Larkin, A. Godoy, D. A. Cavero, C. Sin, J. Waites, Rezaul Mamood, M. Cohron, K. L. Purvis-Roberts

School of Natural Resources: Documents and Reviews

No abstract provided.

Regulation Of P53 By Trpm6 And Trpm7, Thomas Whitbread

Theses and Dissertations

TRPM6 and TRPM7 are two divalent metal cation channels that also possess a serine/threonine kinase domain. They both play critical roles in cellular and organismal metal homeostasis, and are necessary for proper embryonic development and human health. They may function independently in homomeric units, or act together as heterotetramers that display unique characteristics compared to the homomeric channels. These channel-kinases have increasingly been linked to cancer, displaying altered expression levels in a variety of human cancers and contributing to disease progression. Prior work has shown that TRPM6 and TRPM7 can participate in cancer-related signaling pathways. Here, the potential for TRPM6 …

The Location Of Nf-Kb And Akap-95 In Lipopolysaccharide Treated Rt4-D6p2t Schwannoma Cells, Mackenzie Wilcox, Angela Asirvatham

SURF Posters 2023

No abstract provided.

A Tribute To Gary D. Wallace

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

A Vascular Flora Of The South Fork Tule River, Southern Sierra Nevada, Tulare County, California, Jessica M. Orozco

Aliso: A Journal of Systematic and Floristic Botany

I conducted a floristic study of the South Fork Tule River watershed, located in the southern Sierra Nevada in Tulare County, California. The primary objectives of this floristic study were to document and catalogue all vascular plant taxa that occur in the watershed, describe the plant communities, analyze the flora and its affinities with other areas, and establish a herbarium for the Tule River Indian Reservation. The upper reaches of the South Fork Tule River originate on the western slope of Slate Mountain in the Sequoia National Forest and drain west through the Tule River Indian Reservation into Lake Success. …

Comparing The Performance Of A Targeted Pull-Down Assay To Shotgun Sequencing For Improving Respiratory Infectious Disease Surveillance, Monica R. Christian

Browse all Theses and Dissertations

Current surveillance focuses on well characterized pathogens such as influenza. Since 2000, there have been multiple outbreaks of respiratory disease. These outbreaks have demonstrated the need for robust and collaborative global efforts to identify, monitor, and contain novel respiratory viruses. This study aims to improve the ability of public health agencies to monitor and respond to respiratory disease outbreaks. Using five respiratory pathogens, this study compares a molecular capture technology from Twist Biosciences to the shotgun sequencing approach of whole transcriptome amplification (WTA, Qiagen) with the goal of determining which method is most effective using cost, usability, and sequencing quality …

Tolle Lege Works Published In 2023, Mcquade Library

Bibliographies

Compiled Tolle Lege bibliography of works published by members of the Merrimack College Community in 2023. Works include journal articles, books, book chapters, conference presentations and posters, and more.

2023 Annual Report, Tennessee. Division Of Forestry And Tennessee. Department Of Agriculture

Forestry Annual Reports

This annual report presents an overview of Tennessee Forestry Commission programs and management.

Effect Of Operating Parameters On Performance Of Finger Millet Pearling Machine, Ranjit Powar, Vijay Aware, Prasad Chavan, Amit K. Jaiswal

Articles

The effect of operational parameters such as feed rate (FR), drum speed (DS), and concave clearance (CC) was studied on the performance of a pearling drum. The pearling drum consists of pearling cylinder, pearling sieve, concave, and outer casing of the drum. The response surface method with CCRD experimental design was used to study the effect of operational parameters on pearling drum. The considered levels for optimizing operational parameters viz., FR, DS, and CC were 40–80 kg/h, 5– 10 m/s, and 3–15 mm, respectively. The maximum pearling efficiency (PE) and minimum grain damage (GD) were found to be 98.40%, and …

Protected Areas Slow Declines Unevenly Across The Tetrapod Tree Of Life, A Justin Nowakowski, James I. Watling, Alexander Murray, Jessica L. Deichmann, Thomas S. Akre, Carlos L. Muñoz Brenes, Brian D. Todd, Louise Mcrae, Robin Freeman, Luke O. Frishkoff

2023 Faculty Bibliography

Protected areas (PAs) are the primary strategy for slowing terrestrial biodiversity loss. Although expansion of PA coverage is prioritized under the Convention on Biological Diversity, it remains unknown whether PAs mitigate declines across the tetrapod tree of life and to what extent land cover and climate change modify PA effectiveness1,2. Here we analysed rates of change in abundance of 2,239 terrestrial vertebrate populations across the globe. On average, vertebrate populations declined five times more slowly within PAs (−0.4% per year) than at similar sites lacking protection (−1.8% per year). The mitigating effects of PAs varied both within and across vertebrate …

New Terrestrial Cyanobacteria From The Azores Islands: Description Of Venetifunis Gen. Nov. And New Species Of Albertania, Kovacikia And Pegethrix, Ruben Luz, Rita Cordeiro, Jan Kastovsky, Jeffrey R. Johansen, Elisabete Dias, Amelia Fonseca, Vitor Vasconcelos, Vitor Goncalves

2023 Faculty Bibliography

The taxonomy of cyanobacteria has advanced quickly with the use of molecular methods in combination with well-defined morphological and ecological characters. Using this approach, many taxonomical changes have occurred in the Synechococcales and Oscillatoriales, with the description of new families, genera and species. Here we present the study of four cyanobacterial strains from the Bank of Algae and Cyanobacteria of the Azores (BACA) culture collection, all isolated from the Azores Archipelago. The strains were genetically characterized through the amplification of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region, as well as morphologically by light and transmission …

Linking Predator Responses To Alkaloid Variability In Poison Frogs Language:, J P. Lawrence, Bibiana Rojas, Annelise Blanchett, Ralph Saporito, Johanna Mappes, Antoine Fouquet, Brice P. Noonan

2023 Faculty Bibliography

Many chemically-defended/aposematic species rely on diet for sequestering the toxins with which they defend themselves. This dietary acquisition can lead to variable chemical defenses across space, as the community composition of chemical sources is likely to vary across the range of (an aposematic) species. We characterized the alkaloid content of two populations of the Dyeing Poison Frog (Dendrobates tinctorius) in northeastern French Guiana. Additionally, we conducted unpalatability experiments with naive predators, Blue Tits (Cyanistes caeruleus), using whole-skin secretion cocktails to assess how a model predator would respond to the defense of individuals from each population. While there was some overlap …

Maternal Chemical Defenses Predict Ofspring Defenses In A Dendrobatid Poison Frog, Olivia L. Brooks, Jessie J. James, Ralph Saporito

2023 Faculty Bibliography

Within and among populations, alkaloid defenses of the strawberry poison frog (Oophaga pumilio) vary spatially, temporally, and with life history stage. Natural variation in defense has been implicated as a critical factor in determining the level of protection aforded against predators and pathogens. Oophaga pumilio tadpoles sequester alkaloids from nutritive eggs and are, thus, entirely dependent on their mothers for their defense. However, it remains unclear how tadpole alkaloid composition relates to that of its mother and how variation in maternally provisioned defenses might result in varying levels of protection against predators. Here, we demonstrate that natural variation in the …

Albertania And Egbenema Gen. Nov. From Nigeria And The United States, Expanding Biodiversity In The Oculatellaceae (Cyanobacteria), Mildred Akagha, Nicole Pietrasiak, David F. Bustos, Alžběta Vondrášková, Sandra C. Lamb, Jeffrey R. Johansen

2023 Faculty Bibliography

Knowledge of the tropical terrestrial cyanobacterial flora from the African con-tinent is still limited. Of 31 strains isolated from soil and subaerial samples col-lected in Lagos State, Nigeria, three were found to be in the Oculatellaceae,including two species in a new genus. Subsequently, isolates from micro-bial mats in White Sands National Park in New Mexico, United States, andfrom a rock near the ocean in Puerto Rico, United States, were found tobelong to the new genus as well. Cyanobacterial isolates were character-ized microscopically, sequenced for the 16S rRNA gene and associated ITSregion, and phylogenetically analyzed. Egbenema gen. nov., with three newspecies, …

Investigating Peptide Folding Of Tryptophan Zippers, Chad Tatko, Jessica Bedow, Kachel Bedow, Maddy Hoogstra, Douglas Vander Griend

Summer Research

Protein folding has been a subject of interest in the research community for its wide array o f applications in academia and medicine. By understanding how the interaction of localized amino acids in different environmental factors affect the global folding of peptides, one can anticipate synthesis of proteins or manipulation of exis ting pro teins to have more desirable functions. For this investigation, tryptophan zippers (monomeric β-hairpin peptides) were synthesized, titrated in differing pH, and analyzed by NMR

The Emma Cole Grand Rapids Flora Project: Documenting And Assessing Change 123 Years Later, Elliott Stob, Renee Figueroa, Garrett Crow, David Warners

Summer Research

In 1901, botanist, teacher, and Kent Scientific Institute scientist, Emma J. Cole published her book Grand Rapids Flora: A Catalogue of the Flowering Plants and Ferns Growing Without Cultivation in the Vicinity of Grand Rapids, Michigan. For over 100 years her book has provided an excellent perspective into the natural environment of the area. The aim of the project is to revisit and inventory sites she explored, as well as other remnant natural areas, to see how plant life has changed over the past 120+ years.

Beginning in 2015 the project has assessed 54 sites, mentored 19 students, discovered …