Life Sciences Commons^™

Using Art To Foster Creation Care And Advance Environmental Sustainability, Orion Wakeman, Dave Warners

Summer Research

Calvin University is on land that drains into the Plaster Creek watershed, which is known as the most contaminated watershed in West Michigan. Plaster Creek Stewards (PCS) is a local watershed group at Calvin University that uses native plantings and curb-cut rain gardens to address human impact to the watershed, including ten areas on Calvin’s campus. The restored habitats and rain gardens are unique features that still perceived by many as a challenge to traditional aesthetics. I embrace the intersection of arts and sciences to provide PCS and Calvin University with visually compelling resources for the public to learn about …

Molecular Regulation Of The Salicylic Acid Hormone Pathway In Plants Under Changing Environmental Conditions, Christina A. M. Rossi, Eric J. R. Marchetta, Jong Hum Kim, Christian Castroverde

Biology Faculty Publications

Salicylic acid (SA) is a central plant hormone mediating immunity, growth, and development. Recently, studies have highlighted the sensitivity of the SA pathway to changing climatic factors and the plant microbiome. Here we summarize organizing principles and themes in the regulation of SA biosynthesis, signaling, and metabolism by changing abiotic/biotic environments, focusing on molecular nodes governing SA pathway vulnerability or resilience. We especially highlight advances in the thermosensitive mechanisms underpinning SA-mediated immunity, including differential regulation of key transcription factors (e.g., CAMTAs, CBP60g, SARD1, bHLH059), selective protein–protein interactions of the SA receptor NPR1, and dynamic phase separation of the recently identified …

Implementing A Service Dog Registration In The United States, Avary Martin

Lewis Honors College Thesis Collection

The presence of fake service animals in public spaces is increasing every day. These mislabeled dogs are posing a great danger to working service dogs and the rights of the handlers that utilize them. In order to reduce the number of fake service dogs in public and ensure equal accessibility to service dog users, a service dog registration should be implemented across the United States. Requiring a public access test to be conducted before a dog can be registered would ensure that any dog working in public has reached the standard of behavior required to be a service dog. This …

Methadone's Regulatory Thicket, Bridget C.E. Dooling, Laura E. Stanley

Annals of Health Law and Life Sciences

No abstract provided.

Foreword, Micaela Enger

Annals of Health Law and Life Sciences

No abstract provided.

There's A Pill For That: The Supreme Court's Misguided Jurisprudence On Commercial Speech Under The First Amendment And Its Implications On Direct-To-Consumer Advertising Of Prescription Drugs, Benjamin Smith

Annals of Health Law and Life Sciences

No abstract provided.

Serving Two Masters: Conflicts Between Physician Employment Contracts And The Physician's Duty Of Care, Steven L. Hendler, M.D.

Annals of Health Law and Life Sciences

No abstract provided.

Table Of Contents, Annals Of Health Law And Life Sciences

Annals of Health Law and Life Sciences

No abstract provided.

Foreword, Micaela Enger

Annals of Health Law and Life Sciences

No abstract provided.

Limiting Overall Hospital Costs By Capping Out-Of-Network Rates, David Orentlicher, Kyra Morgan, Barak Richman

Annals of Health Law and Life Sciences

No abstract provided.

High Volume Multiplex Staining Of Mouse Model In Alzheimer’S Associated Disease Pathology, Chloe Embry

Lewis Honors College Thesis Collection

Although neurodegenerative diseases are often clinically distinct, they typically share common pathological markers. One of the most common causes of clinical dementia is Alzheimer’s disease (AD). Pathologically, AD is defined by the presence of intercellular tangles composed of hyperphosphorylated tau proteins and extracellular plaques made of abnormally cleaved amyloid-beta proteins. However recent genome-wide association studies have also found that many of the predispositions for AD are located on or near genes highly expressed in microglia. In the healthy CNS, microglia act as the brain’s immune system and are chiefly involved in neuronal support and maintaining homeostasis throughout the CNS. Typically, …

Plant Community Response To Control Of A Dominant Invader At Multiple Scales, Alexander R. B. Goetz

Electronic Theses and Dissertations

Control of invasive Tamarix spp. in riparian systems has long been a priority for management in western North America, but questions remain regarding the response of the broader plant community to different treatments. Here we summarize a series of approaches at different scales to quantify the effects of Tamarix control on characteristics of this ecosystem, including provisioning of wildlife habitat. In the first chapter we analyzed findings of the last 20 years using meta-analysis and found that although treatment has been successful in reducing Tamarix and promoting native vegetation to some extent, most literature reports very small effect sizes and …

Developing Red Fluorescent Zn2+ Sensors And Characterizing Zn2+ Homeostasis In Mucolipidosis Type Iv Disease, Anna M. Dischler

Electronic Theses and Dissertations

Zinc (Zn²⁺) is a divalent, redox-inert metal that plays a vital role in many cellular processes either by acting as a catalytic cofactor or a labile signaling molecule in the cytoplasm. Cytosolic Zn²⁺ levels are tightly regulated to stay within a narrow window of concentrations, but fluctuations in Zn²⁺ signals have been detected in a variety of cells. We developed a genetically encoded, single red fluorescent protein (RFP) based Zn²⁺ sensor, RZnP1, that can be used alongside green-wavelength sensors for the simultaneous detection of different signaling molecules within the same cells or among different subcellular …

The Evolutionary Causes And Consequences Of Mammalian Hybridization, Kelsie E. Hunnicutt

Electronic Theses and Dissertations

The natural recombination of divergent genomes within hybrid offspring provides windows through which we can study evolutionary processes and the dynamics of speciation. In this work, I use a combination of comparative and population genomics on both laboratory crosses of divergent rodent lineages and a hybrid zone of cottontail rabbits in the wild to study the causes and consequences of hybridization in mammals. Hybrid sterility is a common reproductive barrier between species that tends to preferentially manifest in the heterogametic sex and frequently genetically maps to the sex chromosomes. Thus, the sex chromosomes appear to play a special role in …

Determination Of Taxonomic Placement Of Falsely-Branched Taxa In Soils Of San Nicolas Island And Reassessment Of The Tolypothrichaceae., Natalie Soliman

Senior Honors Projects

This study was conducted to determine the taxonomic placement of falsely-branched taxa in the soil crusts of San Nicolas Island, which is the largest of the Channel Islands lying off the coast of California. After microscopic analysis of the strains collected from the island, a phylogenetic analysis of 16S rRNA genes, and an analysis of the 16S-23S ITS region, we have identified that these strains belong to the soil genus Spirirestis, which is in the Tolypothrichaceae family. The Tolypothrichaceae is a wellcharacterized monophyletic lineage of non-attenuated, false-branching heteropolar types containing the genera Spirirestis, Hassallia, Tolypothrix, Coleodesmium, and Rexia. The strains …

Investigating The Role Of Nf-Kb Inhibition On Müller Glia Proliferation Following Injury In The Zebrafish Retina, Danielle Baffa

Celebration of Scholarship 2023

No abstract provided.

Determination Of Taxonomic Placement Of Falsely-Branched Taxa In Soils Of San Nicolas Island And Reassessment Of The Tolypothrichaceae, Natalie Soliman

Celebration of Scholarship 2023

No abstract provided.

Teloportwrapper: A New Tool For Understanding The Dynamic World Of Fungal Telomere Ends, Trey Stansfield

Mahurin Honors College Capstone Experience/Thesis Projects

Telomeres are repetitive DNA sequence motifs found at eukaryote chromosome ends. Telomeres help protect chromosome ends from DNA damage and promote chromosome stability. Chromosomes play important roles in aging, mutation, and cancer. Eukaryotic pathogens also use telomeres to mutate and manage virulence genes. In response to chromosome end breakage newly formed telomeres, called de novo telomeres, are formed to recreate the lost telomere and sub-telomeric regions.

Magnaporthe oryzae is a fungal pathogen which causes wheat blast, a deadly plant disease in wheat. Magnaporthe oryzae is also known for its highly variable sub-regions which show high amounts of induced variability due …

Evaluation Of Ionic Liquid Treatment As An Alternative To Chemical Fixation And Dehydration In The Preparation Of Biofilms For Scanning Electron Microscopy, Molly Shreve

Mahurin Honors College Capstone Experience/Thesis Projects

Scanning electron microscopes (SEM) give researchers the ability to observe very small features on biological specimens at the nanometer scale, which is a much higher resolution than what can be obtained with a light microscope. To be observed under SEM, unlike light microscopes, samples must be dry, conductive, and able to withstand electron bombardment. Preparation methods have been developed that work well for samples composed mainly of proteins and lipids, but these fail for samples that are predominantly carbohydrates, such as bacterial biofilms. When carbohydrates of these biofilms are dehydrated, they tend to shrink and collapse, and their normal hydrated …

Food Security, Factors Influencing Food Acceptance, And Preferred Food Assistance Delivery Modalities: A Survey Of Students Attending A Medium Sized, Primarily Residential Emerging Research Institution, Amy Caroline Martin

Graduate Research Theses & Dissertations

Food pantries are the most common form of food assistance provided on college campuses. However, there are several barriers that prevent college students from utilizing pantry resources, leading to low campus food pantry utilization and awareness. Barriers, poor pantry utilization, and awareness have been well documented. However, there is little information currently available regarding factors that facilitate college students’ acceptance of food assistance, and the food distribution modalities that food insecure college students prefer. The purpose of this study was to determine these factors and preferences so they may be used to improve food assistance programs on college campuses.To investigate …

Alterations In C:N Ratio To Understand Plant Metabolic Pathways In Response To Pressure From Goat Grazing In The Invasive Species: Rhamnus Cathartica, Vivian Marchan

Ignite the Spark Outstanding Undergraduate Research and Scholarship

The high fecundity and high germination rates of Rhamus cathartica serves the species to out-compete regional plant native species. Its high abundance in deciduous forest ecosystems forces species that are unable to utilize R. cathartica as a food source to migrate to areas of lower density, further contributing to the decline in regional biodiversity. Bare soil conditions left as the result of the high concentration of Nitrogen in senesced leaves and leaf litter, cause further amplification of the migration of non-plant species to areas of lower R. cathartica density. Nitrogen is essential to primary and secondary metabolism, thus minimizing the …

Alcohol Consumption And Its Effects On The Liver, Eveline Weinreb

The Science Journal of the Lander College of Arts and Sciences

Thousands of people die each year in the United States from alcohol related deaths. Why does the metabolism of alcohol cause severe damage to the body? Ethanol enters the body in the form of wine, and other alcoholic beverages and travels through the digestive system and then to the liver where it is metabolized. There are a series of steps involved in the breakdown of ethanol, and toxic byproducts are formed such as reactive oxygen species (ROS). These oxygen radicals can lead to damaged deoxyribonucleic acid (DNA) and to the formation of adducts. Chronic alcohol consumption can lead to liver …

Mate Recognition In Genetically Distinct Populations Of The Eastern Red-Backed Salamander (Plethodon Cinereus), Courtney Thomas

Masters Theses

Lineages that have undergone genetic divergence and subsequently experience secondary contact present fascinating situations related to the potential for reproductive isolation. Within species hybrid zones provide unique opportunities to test the strength of reproductive barriers through behavioral experiments when genetic relatedness between groups is established. Among the complex interactions that operate to maintain species boundaries, mate recognition and mate choice are two behaviors that serve to promote evolutionary independence across diverse taxonomic lineages. I explored mate recognition in genetically distinct populations of the Eastern Red-backed Salamander, Plethodon cinereus, a small, abundant, terrestrial salamander with a widespread distribution throughout northeastern United …

“Albertania” And “Egbenema”, Expanding Biodiversity In The Oculatellaceae (Cyanobacteria)., Mildred Akagha

Masters Theses

To conduct molecular studies of cyanobacteria of Lagos, Nigeria, a total of eight soil/subaerial samples were collected in Lagos State, Nigeria, and used to isolate cyanobacterial cultures that were characterized microscopically, sequenced, and phylogenetically analyzed. Within the resulting set of cultures, a number of Synechococcales were observed, particularly belonging to Oculatellaceae. “Egbenema” and two new putative species of the recently described genus Albertania were found. Both genera belong to a supported clade within the Oculatellaceae that includes Trichotorquatus and Komarkovaea. The two new putative species of Albertania, “A. egbensis” and “A. latericola”, were from the same sample, but phylogenetically were …

Plant Viruses Of Agricultural Importance: Current And Future Perspectives Of Virus Disease Management Strategies, Satyanarayana Tatineni, Gary Hein

Department of Plant Pathology: Faculty Publications

Plant viruses cause significant losses in agricultural crops worldwide, affecting the yield and quality of agricultural products. The emergence of novel viruses or variants through genetic evolution and spillover from reservoir host species, changes in agricultural practices, mixed infections with disease synergism, and impacts from global warming pose continuous challenges for the management of epidemics resulting from emerging plant virus diseases. This review describes some of the most devastating virus diseases plus select virus diseases with regional importance in agriculturally important crops that have caused significant yield losses. The lack of curative measures for plant virus infections prompts the use …

Post-Translational Modification Of Proteins Via Ambient Air Pollutants And Endogenous Reactive Species, Rachel Lauren Davey

Electronic Theses and Dissertations

Proteins can react with reactive oxygen species (ROS) and reactive nitrogen species (RNS) to form post-translational modifications (PTMs), which can affect protein structure and function. The formation of 3-nitrotyrosine (NTyr) and dityrosine (DiTyr) upon reaction of proteins with ROS/RNS are two common PTMs studied due to their stability and irreversibility, as well as their ability to enhance the allergenicity of pollen allergens upon formation. Many common techniques used to study the formation of these PTMs can reliably detect the PTMs but can only provide semi-quantitative information due to many assumptions and limitations. In Chapter 2 we present an analysis of …

Identifying The Role Of Phospholipase D1 And Phosphatidic Acid In Exocytosis, Broderick L. Bills

Electronic Theses and Dissertations

Exocytosis is an essential process for intercellular communication in eukaryotic cells. This process involves significant changes in membrane curvature, and lipids and curvature-sensing proteins can assist these processes. One protein in particular, phospholipase D1 (PLD1), and its product, the lipid phosphatidic acid (PA), are involved in multiple exocytic processes. However, PLD1 and PA’s role in this process has remained unclear. In this work, PLD1 and the production of PA were visualized during exocytosis, and PA localization to regions of membrane curvature was established. Together, these results support the hypothesis that PLD1 production of PA stabilizes negative curvature during membrane fusion. …

Comparison Of The Dynamic Response And Fracture Characteristics Of Female And Male Lumbar Spine And Pelvis In Underbody Blast Loading, Hollie Ann Pietsch

Wayne State University Dissertations

Underbody blast (UBB) attacks on US Military vehicles in the recent conflicts resulted in debilitating injuries to mounted Warfighters. Biomechanical research studies in the last decade aimed to understand UBB related injuries to guide protective technology designs for military ground vehicles. The design of protective seats is driven by injuries to the pelvis and lumbar spine, which are aligned with the vertical direction of UBB loading. When the US Military began to open many combat roles to female soldiers in 2016, a need arose to understand the impact of survivability and protection for the female Warfighter population as well. Although …

Investigating The Binding Of G-Quadruplex Dna To Lispro And Bovine Insulin, Sarah E. Bultje, Luke D. Witvliet, Kumar Sinniah

Summer Research

Type 1 Diabetes (T1D) is a disease characterized by the inability to produce insulin. Susceptibility to T1D is complex, involving both genetic and environmental factors. o One of the genetic components involved in insulin gene expression, and suspected T1D susceptibility, is the insulin-linked polymorphic region (ILPR). The ILPR element is located on chromosome 11p15 in the proximal promoter region, 363 base pairs upstream from the gene transcription site. o Other researchers (using fluorescence spectroscopy) and we (using ITC) have demonstrated that insulin binds to G-Quadruplex DNA with µM affinity. Since the DNA sequences in the ILPR form G-Quadruplexes and are …

Three Essays On The U.S. Beef Supply Chain: Production, Marketing, And Price Dynamics, Erdal Erol

Theses and Dissertations--Agricultural Economics

This dissertation contains three essays on select economic components of the U.S. beef industry. The first and second essays concentrate on the different economic problems in beef cattle production. The third essay evaluates the price dynamics and the impact of COVID-19 along the beef supply chain.

The first essay explores the economics of culling decisions in cow-calf operations in the U.S. with a novel application of a dynamic mathematical programming model. The results provide an optimal culling strategy under the base model and a range of optimal strategies that vary with respect to different components such as fertility probabilities, market …