Life Sciences Commons^™

Design Of Quinoa Moisture Rapid Detector Based On Capacitance Method, Qian Xi, Zhi-Yong Zhang, Kai Yuan, Dong-Sheng Guo, Ying-Rui Wu

Food and Machinery

The effects of temperature (5~40 ℃), water content (10.14%~29.55%) and frequency (1~100 kHz) on quinoa capacitance were studied by LCR meter. Based on the multiple regression analysis method, and the optimal excitation frequency (21 kHz) and water content detection model for quinoa water content detection were determined. The minimum system board of MSP430F149 microcontroller, TFT touch-screen, AD5933 impedance measurement chip, self-made concentric circle capacitance sensor and DS18B20 digital temperature sensor were selected to complete the design of the quinoa moisture rapid detector. The accuracy test of the detector showed that the average relative error of quinoa water content test results …

Multi-Objective Lightweight Design For Big Arm Of Md-1200yj Palletizing Robot, Ying He, Han Gao, Chao Ma, Li Gu

Food and Machinery

Finite element model of big arm of MD-1200YJ palletizing robot was established,the accuracy of the finite element model was verified by modal test.By static analysis taking into account dynamic factors, constrained modal analysis, vibration response test and frequency response analysis to determine to use minimal mass, maximizing the first and second natural frequency, minimizing the maximal stress, minimizing the maximal deformation and as the optimization objectives. Taking structure parameters as design variables and boundary conditionsof design variables as the constraint conditions, the approximation models of objective functions and constraint functions were established by the Box-Behnken and the RSM. The optimal …

Design Of Weighing System For Micro Mixing Powdery Particles, Da-Pan Hou, Ju-Dong Liu

Food and Machinery

To improve the weighing accuracy and efficiency of mixed powder granules, the material characteristics of the mixed powder granules were studied. The material characteristics of mixed powdery particles are studied. According to the influence of the density, binding property and quantitative size of the mixed materials on the accuracy of quantitative feeding, a method of combined calculation after volumetric quantitative molding is proposed to solve the quantitative error. Studed The influence of the combination model on the combination quantitative error, and the influence of the single bucket quantitative error on the combination process in the combination process. The volume forming …

Expression, Strengthening And Development Of Color Association In Food Packaging Design, Li Xia

Food and Machinery

Food packaging with strong color association can not only transmit food information, but also let consumers understand the cultural connotation behind the product and attract consumers' attention. Taking the associativity of color as the starting point, analyzing the color elements in food packaging based on the relevant theories of color psychology, enhancing the color impact of modern food packaging design, and enhancing the visual experience of consumers, with creating more social value, optimize the color experience of consumers and provide color services for brand extension.

Optimization Of Extraction Process Of Lignans And Polyphenols By Ultrasonic Wave Assisted Extraction From Cinnamomum Camphora, Hai-Xu Zhou, Mei-Yu Xie, Han Gao, Bo Li, Tong-Chao Su, Zhong-Hai Li

Food and Machinery

Response surface methodology (RSM) based on Box Behnken Design (BBD) was employed to optimize the extraction conditions including the liquid-to-solid ratio, ultrasonic time, ethanol concentration and extraction time. The optimized conditions were as follows: liquid-to-solid ratio 1∶22 (g/mL), ultrasonic time 3 min, ethanol concentration 80% and extraction time 78 min. Under these conditions, the experimental yield of lignans and polyphenol were 44.89 mg/g, 48.17 mg/g, respectively.

Establishment And Detection Of Moisture Prediction Model Of Key Processes Of Cigarette Cutting Process, Zi-Juan Li, Bo Liu, Yang Gao, Jiao-Jiao Chen

Food and Machinery

Taking the loosening and conditioning process, feeding moisture returning process, hot air moistening process and the whole silk making process as the research objects, the artificial neural network and multiple regression modeling method were used to investigate the influence of different modeling methods on the moisture prediction accuracy of each process. moreover, the predicted results were tested experimentally. The experimental results showed that the mean of the absolute value of the prediction error was 0.24% for the loose moisture returning process with multiple regression modeling method. The mean of the absolute value of the prediction error was 0.20% for feeding …

Detection Method Of Mutton Adulteration Based On Pso-Lssvm And Characteristic Wavelengths Extraction, Tian-Tian Cheng, Ke-Jian Wang, Xian-Zhong Han, Shi Li, Yuan Wang

Food and Machinery

In order to solve the problem of fast detection of adulteration of mutton and pork, the spectral collection of adulterated mutton was carried out by using a multi-spectral instrument, and the reflectivity of samples at the band of 350~1 100 nm was obtained. For data preprocessing, Particle Swarm Optimization (PSO) was used to optimize the Least Squares Support Vector Machine (LSSVM), and a Least Squares Support Vector Machine (PSO-LSSVM) model based on Particle Swarm Optimization was established, compared with Partial Least Squares(PLS), Back Propagation Neural Network (BPNN) and LSSVM models. The result showed that PSO algorithm could effectively optimize LSSVM …

Geographical Origin Traceability Discrimination Of Chongming Rice Wine, Xing Liu, Qai-Cai Zhang, Chun-Xia Yao, Wei-Guo Song

Food and Machinery

Shanghai Chongming rice wine was used as the main research subject in this study. The natural abundance of hydrogen and oxygen stable isotopes (δ²H and δ¹⁸O values), mineral elements and free amino acids content were analyzed. The discriminant models of Chongming rice wines were established and the characteristic variables were screened by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The results showed that the δ²H and δ¹⁸O values, mineral elements and free amino acids content in rice wines from different geographic origins were different, but only δ^{2 …}

Design And Simulation Of Coffee Capsule Aluminum Foil Welding Equipment, Jing-Feng Ding, Xiao-Zuo Xu, Jiang Xu

Food and Machinery

In order to improve the welding quality of the aluminum foil at the bottom of the coffee capsule, a coffee capsule aluminum foil welding device based on a grooved cam drive was designed. The key transmission system of coffee capsule aluminum foil welding equipment was designed in detail, including the transmission system structure design, the calculation of groove cam motion curve, the derivation of convex contour curve, etc. In ADAMS, the key transmission system model in aluminum foil welding equipment was established, and a simulation environment was built to perform kinematic analysis on the designed transmission system. Rigid-flexible coupling analysis …

Braf V600e-Mutant Cancers Treated With Vemurafenib Alone Or In Combination With Everolimus, Sorafenib, Or Crizotinib Or With Paclitaxel And Carboplatin (Vem-Plus) Study, Blessie Elizabeth Nelson, Jason Roszik, Filip Janku, David S Hong, Shumei Kato, Aung Naing, Sarina Piha-Paul, Siqing Fu, Apostolia Tsimberidou, Maria Cabanillas, Naifa Lamki Busaidy, Milind Javle, Lauren Averett Byers, John V Heymach, Funda Meric-Bernstam, Vivek Subbiah

Faculty, Staff and Student Publications

Combined BRAF + MEK inhibition is FDA approved for BRAF V600E-mutant solid tumors except for colorectal cancer. However, beyond MAPK mediated resistance several other mechanisms of resistance such as activation of CRAF, ARAF, MET, P13K/AKT/mTOR pathway exist among other complex pathways. In the VEM-PLUS study, we performed a pooled analysis of four phase one studies evaluating the safety and efficacy of vemurafenib monotherapy and vemurafenib combined with targeted therapies (sorafenib, crizotinib, or everolimus) or carboplatin plus paclitaxel in advanced solid tumors harboring BRAF V600 mutations. When vemurafenib monotherapy was compared with the combination regimens, no significant differences in OS or …

Effect Of Residual Sugar In High Sugar Grass Silages On Aerobic Stability, G. Pahlow, R. J. Merry, P. O'Kiely, T. M. Pauly, J. M. Greef

IGC Proceedings (1977-2023)

New varieties of Lolium perenne, bred for high sugar content, can contain up to 30% of water soluble carbohydrates (WSC). Only a fraction of such high contents are metabolised during a normal fermentation and the high residual sugar content (RSC) of these silages can improve the efficiency of use of nitrogen by ruminants. However, these RSC at opening for feed-out could be preferentially metabolised relative to fermentation products by all aerobically growing fungi and bacteria present on the forage. A high RSC thus can increase the risk of aerobic deterioration over that of extensively fermented silages, containing predominantly organic …

Recent Developments In Methods To Characterise The Chemical And Biological Parameters Of Grass Silage, R. S. Park, R. E. Agnew, M. G. Porter

IGC Proceedings (1977-2023)

Key points

1. Chemical analysis of forages is expensive, time consuming, environmentally unfriendly and relates poorly to the feed value for production purposes.
2. In vivo characterisation of animal feed is not a feasible option in terms of cost and analysis time.
3. NIRS is a rapid, non destructive, environmentally friendly, multi-analytical technique which can estimate the nutritive value of the feed.
4. NIRS predictive equations developed on a master instrument can be transferred to local and international sites.
5. Future assessment of forages necessitates rapid, stable, instrumentation for ‘in field’ studies.

The Effect Of Chop Length And Additive On Silage Intake And Milk Production In Cows, V. Toivonen, T. Heikkilä

IGC Proceedings (1977-2023)

Effects of reduced silage chop length on silage intake and milk production by dairy cows have been variable. Chopping of grass at harvest generally improves silage fermentation quality and consequently intake. The objective of this study was to assess the effect of chopping flail harvested silage ensiled with acid or biological additive prior to feeding on intake, milk yield and milk composition in dairy cows.

Evaluation Of Narrow-Row Forage Maize In Field-Scale Studies, W. J. Cox, J. H. Cherney, D. J. R. Cherney

IGC Proceedings (1977-2023)

Some dairy producers in the north-eastern USA adopted narrow row (0.38 m) maize forage production in the mid-1990s because of its 5% dry matter (DM) yield advantage (Cox et al., 1998). These dairy producers, however, continued to plant forage maize at high plant densities (125,000 plants/ha) under high N fertility (225 kg N/ha), despite research that indicated that forage maize had optimum DM yields and forage quality when planted at the recommended 100,000 plants/ha under 175 kg/ha of N fertility (Cox & Cherney, 2001). We evaluated forage maize at 0.38 and 0.76 m (conventional) row spacing under recommended vs. …

The Feeding Value Of Conserved Whole-Crop Wheat And Forage Maize Relative To Grass Silage And Ad-Libitum Concentrates For Beef Cattle, K. Walsh, P. O'Kiely, F. O'Mara

IGC Proceedings (1977-2023)

Grass is the predominant forage ensiled in Ireland. However, the relatively modest yields achieved in a single harvest allied to variability in digestibility and ensilability (and thus in intake and animal performance response) and the likelihood of effluent production create disadvantages for grass silage compared to the potential of some alternative forage crops. Thus, alternative forages are worthy of consideration on many farms. The objectives of this study were to quantify the relative intake, digestibility and performance of beef cattle offered grass silage, forage maize silage and whole-crop wheat (fermented or urea-treated), rank these relative to cattle offered an ad …

Ensiling Safflower (Carthamus Tinctorius) As An Alternative Winter Forage Crop In Israel, Z. G. Weinberg, S. Y. Landau, A. Bat-Tal, Y. Chen, M. Gamburg, S. Brener, L. Devash

IGC Proceedings (1977-2023)

Israel is a subtropical country in which the rainy season is in winter, with frequent droughts. Wheat is the major winter forage crop in Israel, along with legumes as rotation crops. Alternative forage crops are sought that would be suitable for semi-arid areas. Safflower (Carthamus tinctorius) is usually grown as a source for oil and pigments but spineless cultivars could be used as fodder. Leshem et al. (2001) reported DM yields up to 22 t/ha and high DM digestibility when used for heifers. When safflower silage substituted maize and wheat silage in the rations of lactating cows, …

Effects Of Two Different Chopping Lengths Of Maize Silage On Silage Quality And Dairy Performance, K. Mahlkow, J. Thaysen

IGC Proceedings (1977-2023)

Maize silage harvested at dough stage contains less fibre structure than grass silage. Due to higher proportions of maize silage in dairy rations and due to the fact that with longer chopping length of maize silage the contribution of fibre structure increases. The objective of this study was to assess the effect of two chopping lengths in the silage on animal performance with respect to feed intake, milk yields and ingredients as well as physiological effects of digestion.

Effect Of Additives At Harvest On The Digestibility In Lambs Of Whole Crop Barley Or Wheat Silage, S. Muhonen, I. Olsson, P. Lingvall

IGC Proceedings (1977-2023)

There are very few published articles about how silage additives affect digestibility of whole crop silage. In this experiment, male lambs were given whole crop barley or wheat silage harvested at dough stage with a number of different acid-based and bacterial additives.

Use Of Silage Additives In Ensiling Of Whole-Crop Barley And Wheat - A Comparison Of Round Big Bales And Precision Chopped Silages, M. Knický, P. Lingvall

IGC Proceedings (1977-2023)

An increasing use of whole-crop cereals, as supplementary feed, has increased interest in development of efficient ways of preserving these forages to achieve a high hygienic quality. It is known that ensiling of whole-cereals often results in silages with high concentrations of butyric acid (Weissbach & Haacker, 1988). Furthermore, problems with poor aerobic stability still persist despite the use of lactic acid bacteria (Filya et al., 2000). Therefore, the objective of this study was to examine the effect of different types of additive mixtures on the fermentation process and aerobic stability of precision chopped and baled silages.

The Santa Clara, 2023-02-17, Santa Clara University

The Santa Clara

No abstract provided.

Acetylcholinesterase Activity Staining In Freshwater Planarians, Christina Rabeler, Taixi Gong , '24, Danielle Ireland, O. Cochet-Escartin, Eva-Maria S. Collins

Biology Faculty Works

The serine hydrolase acetylcholinesterase (AChE) is an important neuronal enzyme which catalyzes the hydrolysis of the neurotransmitter acetylcholine and other choline esters. The breakdown of acetylcholine by AChE terminates synaptic transmission and regulates neuromuscular communication. AChE inhibition is a common mode of action of various insecticides, such as carbamates and organophosphorus pesticides. Freshwater planarians, especially the species Dugesia japonica, have been shown to possess AChE activity and to be a suitable alternative model for studying the effects of pesticides in vivo. AChE activity can be quantified in homogenates using the Ellman assay. However, this biochemical assay requires specialized equipment …

Tracking The Evolution Of Esophageal Squamous Cell Carcinoma Under Dynamic Immune Selection By Multi-Omics Sequencing, Sijia Cui, Nicholas Mcgranahan, Jing Gao, Peng Chen, Wei Jiang, Lingrong Yang, Li Ma, Junfang Liao, Tian Xie, Congying Xie, Tariq Enver, Shixiu Wu

Children’s Nutrition Research Center Staff Publications

Intratumoral heterogeneity (ITH) has been linked to decreased efficacy of clinical treatments. However, although genomic ITH has been characterized in genetic, transcriptomic and epigenetic alterations are hallmarks of esophageal squamous cell carcinoma (ESCC), the extent to which these are heterogeneous in ESCC has not been explored in a unified framework. Further, the extent to which tumor-infiltrated T lymphocytes are directed against cancer cells, but how the immune infiltration acts as a selective force to shape the clonal evolution of ESCC is unclear. In this study, we perform multi-omic sequencing on 186 samples from 36 primary ESCC patients. Through multi-omics analyses, …

Exploiting Prmt5 As A Target For Combination Therapy In Mantle Cell Lymphoma Characterized By Frequent Atm And Tp53 Mutations, Yuxuan Che, Yang Liu, Yixin Yao, Holly A Hill, Yijing Li, Qingsong Cai, Fangfang Yan, Preetesh Jain, Wei Wang, Lixin Rui, Michael Wang

Faculty, Staff and Student Publications

Constant challenges for the treatment of mantle cell lymphoma (MCL) remain to be recurrent relapses and therapy resistance, especially in patients harboring somatic mutations in the tumor suppressors ATM and TP53, which are accumulated as therapy resistance emerges and the disease progresses, consistent with our OncoPrint results that ATM and TP53 alterations were most frequent in relapsed/refractory (R/R) MCL. We demonstrated that protein arginine methyltransferase-5 (PRMT5) was upregulated in R/R MCL, which predicted a poor prognosis. PRMT5 inhibitors displayed profound antitumor effects in the mouse models of MCL with mutated ATM and/or TP53, or refractory to CD19-targeted CAR T-cell therapy. …

Cd5 Expression By Dendritic Cells Directs T Cell Immunity And Sustains Immunotherapy Responses, Mingyu He, Kate Roussak, Feiyang Ma, Nicholas Borcherding, Vince Garin, Mike White, Charles Schutt, Trine I Jensen, Yun Zhao, Courtney A Iberg, Kairav Shah, Himanshi Bhatia, Daniel Korenfeld, Sabrina Dinkel, Judah Gray, Alina Ulezko Antonova, Stephen Ferris, David Donermeyer, Cecilia Lindestam Arlehamn, Matthew M Gubin, Jingqin Luo, Laurent Gorvel, Matteo Pellegrini, Alessandro Sette, Thomas Tung, Rasmus Bak, Robert L Modlin, Ryan C Fields, Robert D Schreiber, Paul M Allen, Eynav Klechevsky

Faculty, Staff and Student Publications

The induction of proinflammatory T cells by dendritic cell (DC) subtypes is critical for antitumor responses and effective immune checkpoint blockade (ICB) therapy. Here, we show that human CD1c+CD5+ DCs are reduced in melanoma-affected lymph nodes, with CD5 expression on DCs correlating with patient survival. Activating CD5 on DCs enhanced T cell priming and improved survival after ICB therapy. CD5+ DC numbers increased during ICB therapy, and low interleukin-6 (IL-6) concentrations promoted their de novo differentiation. Mechanistically, CD5 expression by DCs was required to generate optimally protective CD5hi T helper and CD8+ T cells; further, deletion of CD5 from T …

Drug-Like Small Molecules That Inhibit Expression Of The Oncogenic Microrna-21, Matthew D Shortridge, Bhawna Chaubey, Huanyu J Zhang, Thomas Pavelitz, Venkata Vidadala, Changyan Tang, Gregory L Olsen, George A Calin, Gabriele Varani

Faculty, Staff and Student Publications

We report the discovery of drug-like small molecules that bind specifically to the precursor of the oncogenic and pro-inflammatory microRNA-21 with mid-nanomolar affinity. The small molecules target a local structure at the Dicer cleavage site and induce distinctive structural changes in the RNA, which correlate with specific inhibition of miRNA processing. Structurally conservative single nucleotide substitutions eliminate the conformational change induced by the small molecules, which is also not observed in other miRNA precursors. The most potent of these compounds reduces cellular proliferation and miR-21 levels in cancer cell lines without inhibiting kinases or classical receptors, while closely related compounds …

Was Kaposi’S Sarcoma-Associated Herpesvirus Introduced Into China Via The Ancient Silk Road? An Evolutionary Perspective, Zhenqiu Liu, Qiwen Fang, Jialu Zuo, Veenu Minhas, Charles Wood, Na He, Tiejun Zhang

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) has become widely dispersed worldwide since it was first reported in 1994, but the seroprevalence of KSHV varies geographically. KSHV is relatively ubiquitous in Mediterranean areas and the Xinjiang Uygur Autonomous Region, China. The origin of KSHV has long been puzzling. In the present study, we collected and analysed 154 KSHV ORF-K1 sequences obtained from samples originating from Xinjiang, Italy, Greece, Iran and southern Siberia using Bayesian evolutionary analysis in BEAST to test the hypothesis that KSHV was introduced into Xinjiang via the ancient Silk Road. According to the phylogenetic analysis, 72 sequences were subtype A …

An Innovative Approach To Improve Oil Production And Quality Of Mustard (Brassica Juncea L.) With Multi-Nutrient-Rich Polyhalite, Biswajit Pramanick, B.S. Mahapatra, Debarati Datta, Prithwiraj Dey, S.P. Singh, Ajay Kumar, Bappa Paramanik, Neeraj Awasthi

Department of Agronomy and Horticulture: Faculty Publications

Polyhalite popularly known as POLY4 is a multi-nutrient fertiliser containing K, S, Mg, Ca, and micronutrients. POLY4 has a low carbon footprint, is certified for organic agriculture, and has the potential to improve crop productivity and quality attributes Indian mustard which often faces challenges due to imbalanced nutrition supplied in the current fertilisation schedule. The hypothesis of the study was that the multi-nutrient fartiliser POLY4 can ensure balanced nutrition for Indian mustard. Considering this, a field experiment was conducted during the winter seasons of 2017–18 and 2018–19 to evaluate the effect of POLY4 on Indian mustard (Brassica juncea L.) …

Adjuvant Chemotherapy Versus Adjuvant Concurrent Chemoradiotherapy After Radical Surgery For Early-Stage Cervical Cancer: A Randomized, Non-Inferiority, Multicenter Trial, Danhui Weng, Huihua Xiong, Changkun Zhu, Xiaoyun Wan, Yaxia Chen, Xinyu Wang, Youzhong Zhang, Jie Jiang, Xi Zhang, Qinglei Gao, Gang Chen, Hui Xing, Changyu Wang, Kezhen Li, Yaheng Chen, Yuyan Mao, Dongxiao Hu, Zimin Pan, Qingqin Chen, Baoxia Cui, Kun Song, Cunjian Yi, Guangcai Peng, Xiaobing Han, Ruifang An, Liangsheng Fan, Wei Wang, Tingchuan Xiong, Yile Chen, Zhenzi Tang, Lin Li, Xingsheng Yang, Xiaodong Cheng, Weiguo Lu, Hui Wang, Beihua Kong, Xing Xie, Ding Ma

Faculty, Staff and Student Publications

We conducted a prospective study to assess the non-inferiority of adjuvant chemotherapy alone versus adjuvant concurrent chemoradiotherapy (CCRT) as an alternative strategy for patients with early-stage (FIGO 2009 stage IB-IIA) cervical cancer having risk factors after surgery. The condition was assessed in terms of prognosis, adverse effects, and quality of life. This randomized trial involved nine centers across China. Eligible patients were randomized to receive adjuvant chemotherapy or CCRT after surgery. The primary end-point was progression-free survival (PFS). From December 2012 to December 2014, 337 patients were subjected to randomization. Final analysis included 329 patients, including 165 in the adjuvant …

Exploiting Metabolic Vulnerabilities After Anti-Vegf Antibody Therapy In Ovarian Cancer, Deanna Glassman, Mark S Kim, Meredith Spradlin, Sunil Badal, Mana Taki, Pratip Bhattacharya, Prasanta Dutta, Charles V Kingsley, Katherine I Foster, Olamide Animasahun, Jin Heon Jeon, Abhinav Achreja, Anusha Jayaraman, Praveen Kumar, Minal Nenwani, Fulei Wuchu, Emine Bayraktar, Yutuan Wu, Elaine Stur, Lingegowda Mangala, Sanghoon Lee, Timothy A Yap, Shannon N Westin, Livia S Eberlin, Deepak Nagrath, Anil K Sood

Faculty, Staff and Student Publications

Despite modest clinical improvement with anti-vascular endothelial growth factor antibody (AVA) therapy in ovarian cancer, adaptive resistance is ubiquitous and additional options are limited. A dependence on glutamine metabolism, via the enzyme glutaminase (GLS), is a known mechanism of adaptive resistance and we aimed to investigate the utility of a GLS inhibitor (GLSi). Our in vitro findings demonstrated increased glutamine abundance and a significant cytotoxic effect in AVA-resistant tumors when GLSi was administered in combination with bevacizumab. In vivo, GLSi led to a reduction in tumor growth as monotherapy and when combined with AVA. Furthermore, GLSi initiated after the emergence …

Endothelial-To-Osteoblast Transition In Normal Mouse Bone Development, Song-Chang Lin, Guoyu Yu, Yu-Chen Lee, Jian H Song, Xingzhi Song, Jianhua Zhang, Theocharis Panaretakis, Christopher J Logothetis, Yoshihiro Komatsu, Li-Yuan Yu-Lee, Guocan Wang, Sue-Hwa Lin

Faculty, Staff and Student Publications

Metastatic prostate cancer (PCa) in bone induces bone-forming lesions. We have previously shown that PCa-induced bone originates from endothelial cells (ECs) that have undergone EC-to-osteoblast (OSB) transition. Here, we investigated whether EC-to-OSB transition also occurs during normal bone formation. We developed an EC and OSB dual-color reporter mouse (DRM) model that marks EC-OSB hybrid cells with red and green fluorescent proteins. We observed EC-to-OSB transition (RFP and GFP co-expression) in both endochondral and intramembranous bone formation during embryonic development and in adults. Co-expression was confirmed in cells isolated from DRM. Bone marrow– and lung-derived ECs underwent transition to OSBs and …