Life Sciences Commons^™

Emerging Treatments For Myelodysplastic Syndromes: Biological Rationales And Clinical Translation, Juan Jose Rodriguez-Sevilla, Vera Adema, Guillermo Garcia-Manero, Simona Colla

Faculty, Staff and Student Publications

Myelodysplastic syndromes (MDSs) are a heterogeneous group of clonal hematopoietic stem cell disorders characterized by myeloid dysplasia, peripheral blood cytopenias, and increased risk of progression to acute myeloid leukemia (AML). The standard of care for patients with MDS is hypomethylating agent (HMA)-based therapy; however, nearly 50% of patients have no response to the treatment. Patients with MDS in whom HMA therapy has failed have a dismal prognosis and no approved second-line therapy options, so enrollment in clinical trials of experimental agents represents these patients' only chance for improved outcomes. A better understanding of the molecular and biological mechanisms underpinning MDS …

Comparison Of Dna Extraction Methods For Soft Tissue Samples, Steve Guzman, Nathaniel E. Hill, Mujtaba Shah, Jonathon Reynolds, Cole Farnsworth, Molly Henley, Alfred Amendolara, Laura Minor, John Dougherty Jr., John A. Kriak, Kyle B. Bills, David W. Sant

Annual Research Symposium

No abstract provided.

Vitamin C Contributes To Epigenetic Regulation Of Genes Related To Diabetic Retinopathy In Retinal Endothelial Cells, Elizabeth L. Turner, Jonathon Reynolds, Walker Kay, Marianne Becnel, Matthew Conway, Alexander Kim, John A. Kriak, Kyle B. Bills, David W. Sant

Annual Research Symposium

No abstract provided.

Determination Of Feasibility And Plausibility Of Using Various Biological Samples For Remote Genetic Testing, Cole Farnsworth, Molly Henley, Walker Kay, Varos Victer Manukyan, Jonathon Reynolds, John A. Kriak, Kyle B. Bills, David W. Sant

Annual Research Symposium

No abstract provided.

Development Of A Method For Identifying And Quantifying Epicatechin In Cinnamon Extract Supplement Capsules, Danielle Valls

Annual Research Symposium

No abstract provided.

Detection And Relative Quantitation Of Changes In Gene Expression Of Heat Shock Proteins 27 (Hsp27), Hsp 70, Hsp 90 And Hsp 47 In Doxorubicin-Exposed Human Cells By Rt-Qpcr, Steve Lee, Christopher So Dr.

Annual Research Symposium

Gene expression

Cell cultures

Heat shock proteins

The Immunomodulating Effects Of Morphine Dependence And Withdrawal In A Rat Model, Mark Stoll

Annual Research Symposium

Would you like to know what happens to the immune system during opioid drug withdrawal?

The Immunomodulating Effects Of Delta-9 Tetrahydrocannabinol (Thc) And Cannabidiol (Cbd) In The Context Of Infection, Rose D. Goodman, Kate Rouse, Victor Jimenez

Annual Research Symposium

No abstract provided.

Development Of An Lcms Method To Detect And Quantify Curcumin In A Novel Oral Formulation Of Turmeric, Brandon Renninger

Annual Research Symposium

No abstract provided.

Sulforaphane Pre-Treatment Improves Cytoprotection Against Opportunistic Pathogens, Caleb Harrop, Nathan Clark

Annual Research Symposium

No abstract provided.

Effects Of B4galnt1 Expression On Metastatic Phenotype And Response To Treatment In Osteosarcoma Cell Lines, Fatemeh Zareihajiabadi

Annual Research Symposium

No abstract provided.

A Composition Of Phytonutrients For Glycemic And Weight Management, Yasuyo Urasaki

Annual Research Symposium

No abstract provided.

Effects Of Post-Translational Histone Modifications On Transcription Rate, Aaron Bohn

Annual Research Symposium

No abstract provided.

The Role Of The Kcc2 In Substance Use And Abuse: A Systematic Review [Protocol], Alfred Amendolara, Steven Salazar, Chad Thompson, Hyrum Wright, Andrew Payne

Annual Research Symposium

This poster presents the protocol for an ongoing systematic review investigating the role of potassium chloride co-transporter 2 (KCC2) in substance use, abuse, and addiction.

A Systematic Review: Toxoplasma Gondii Infection And Drugs Of Abuse, Amani Sastry

Annual Research Symposium

No abstract provided.

Appearance Of Two Distinct Clusters Of Glucose Dysregulation In Chronic Intractable Migraine, Roshni Jogin, Kaitlyn Mcfarland, Aparna Naik, Tanner Williford, Matthew Conway, Amar Paul, John A. Kriak, David W. Sant, Kyle B. Bills

Annual Research Symposium

No abstract provided.

Heterodyned Whole-Body Vibration Ameliorates Anxiety In Opioid-Use Disorder, Kailee Edwards, Braden Cunningham, Alfred Amendolara, Marryam Anwar, Jon Gonzales, Roshni Jogin, Wyatt Magoffin, Amar Paul, Ryan Perry, James Pike, Nathan Swallow, Steven Tung, Mary Seamons, Andrew Payne, John A. Kriak, David W. Sant, David W. Sant

Annual Research Symposium

No abstract provided.

Development Of A Neural Network Model To Identify Abnormalities In Cervical X-Rays, Alex P. Sheppert, Nasif Islam, Race Peterson, Michael T. Sullivan, John A. Kriak, David W. Sant, Kyle B. Bills

Annual Research Symposium

No abstract provided.

Presentation Of Paired P- And Q-Arm Mosaic Deletions On Chromosome 18 Associated With Neuropsychiatric Symptoms, Jackson Nielsen, Laura Minor, John Dougherty Jr., Paige Moore, Kailee Edwards, Brandon Burrell, Jameson Williams, John A. Kriak, David W. Sant, Kyle B. Bills

Annual Research Symposium

No abstract provided.

The Effects Of Maize And Whole Crop Wheat Silages And Quality Of Grass Silage On The Performance Of Lactating Dairy Cows, D. C. Patterson, D. J. Kilpatrick

IGC Proceedings (1977-2023)

Patterson et al. (2004) obtained positive milk production responses to the inclusion of maize silage in grass silage-based diets under Northern Ireland conditions. By contrast, while inclusion of fermented whole crop wheat increased total forage intake, it had no significant effect on milk production of dairy cows. More recently, a newer technique has been developed of harvesting the wheat crop at high DM content, with milling of the grain during harvesting and treatment with an urea/urease mixture at ensiling (alkalage treatment). The aim of the present study was to investigate the milk production potential of high DM whole crop …

Polyphenol Oxidase Activity And In Vitro Proteolytic Inhibition In Grasses, J. M. Marita, R. D. Hatfield, G. E. Brink

IGC Proceedings (1977-2023)

Harvesting and storing high quality forage in the cool humid regions remains a challenge due to the potential for protein degradation during ensiling. Red clover is an exception as high protein levels are maintained during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This project was undertaken to determine if PPO activity is present in a range of grasses and the potential role in proteolytic inhibition in the presence of the o-diphenol caffeic acid.

The Effect Of Dry Matter Content And Inoculation With Lactic Acid Bacteria On The Residual Water Soluble Carbohydrate Content Of Silages Prepared From A High Sugar Grass Cultivar, D. R. Davies, D. K. Leemans, E. L. Bakewell, R. J. Merry

IGC Proceedings (1977-2023)

The introduction of new perennial ryegrass cultivars bred for high water soluble carbohydrate (WSC) content has created opportunities for improving the quality of grass silage, by not only providing adequate WSC for a good fermentation, but also sufficient to leave a higher residual level of WSC in the mature silage. High WSC silages have the potential to provide readily available energy during the early stages of rumen fermentation to balance energy and nitrogen supply and optimise rumen microbial growth. (Merry et al. 2002). The aim was to examine the effect of wilting and silage inoculants on the residual WSC content …

Using The Red Clover Polyphenol Oxidase Gene To Inhibit Proteolytic Activity In Lucerne, R. D. Hatfield, M. L. Sullivan, R. E. Muck

IGC Proceedings (1977-2023)

Preserving high quality forage in cool humid regions of agricultural production remains a challenge due to potentially high levels of protein degradation during ensiling. Red clover is an exception maintaining its high protein levels during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This work highlights potential strategies for utilising PPO as a means of decreasing proteolytic degradation during the ensiling of lucerne and other forages.

New Results On Inhibition Of Clostridia Development In Silages, E. Kaiser, K. Weiß, I. Polip

IGC Proceedings (1977-2023)

The prevention of clostridial activity in silages is one of the most important aims in silage making. Clostridial activity in silages is especially expressed as the occurrence of butyric acid and as increased content of clostridial spores. A rapid reduction in the pH value at the beginning of fermentation process is considered as the most important factor for inhibition of clostridial development. It is assumed, that, if the “critical pH value” will be quickly achieved, clostridial activity in silages can be stopped. In experiments concerning the fermentation process it was found that the effect of acidification and dry matter content …

Ensilability And Silage Quality Of Different Cocksfoot Varieties, U. Wyss

IGC Proceedings (1977-2023)

Various factors determine the ensilability of plant crops. In addition to dry matter content, sugar and protein content, buffering capacity, plant structure, soil contaminations and epiphytic microflora are also important. In Switzerland, various mixtures and also pure swards are continuously revised on the basis of results from variety testing programs. The main parameters for these tests are the yield and nutrient contents. However, in terms of ensilability and silage quality, no systematic tests have been carried out. For this reason, we have tested the ensilability of different cocksfoot varieties as well as the quality of the silages.

Effects Of Silage Preparation And Microbial Silage Additives On Biogas Production From Whole Crop Maize Silage, M. Neureiter, C. Perez Lopez, H. Pichler, R. Kirchmayr, R. Braun

IGC Proceedings (1977-2023)

Biogas applications based on the production of energy from renewable resources have emerged in the past years due to several countries setting quotas for bioenergy thus promoting anaerobic digestion for heat and electricity generation. Maize is one of the most common substrates for biogas production based on energy crops because of the high yields per hectare with ensiling as the preferred method for storage. Experiments were performed to investigate whether conditions during the silage fermentation and the addition of starter cultures can affect the biogas yields.

Ensiling Characteristics Of Sudangrass Silage Treated With Green Tea Leaf Waste Or Green Tea Polyphenols, M. Kondo, K. Kita, H. Yokota

IGC Proceedings (1977-2023)

Green tea waste (GTW), emitted from beverage companies manufacturing tea drinks, contains high crude protein (CP) and polyphenols. Kondo et al. (2004) showed that GTW addition to forage ensiling enhanced lactic acid fermentation and decreased pH value. Ishihara et al. (2001) showed that high counts of Lactobacillus species were maintained and the counts of clostridia were decreased in the intestinal microflora of animals fed the diet containing green tea polyphenols (GTP). It is hypothesised that GTP might activate lactic acid bacteria and enhance silage fermentation. This study was conducted to evaluate the potential of GTW and GTP as silage …

A 16s Rdna-Based Quantitative Assay For Monitoring Lactobacillus Plantarum In Silage, M. Klocke, K. Mundt, C. Idler, P. O'Kiely, S. Barth

IGC Proceedings (1977-2023)

Ensilage of herbaceous biomass can be enhanced by applying pre-selected fermentative bacteria, however insufficient is known about the population dynamics of such starter cultures under a range of ensiling conditions. Classical methods for species-specific quantification of bacteria are labour intensive. An alternative approach is the detection of bacteria based on molecular markers for species-specific regions within their genomic DNA (e.g. the 16S rDNA sequence). In this study, a quantitative marker assay using the real-time PCR technique (Q-PCR) is described for Lactobacillus plantarum, a bacterium often used for silage starter cultures.

A Comparison Of The Efficacy Of An Ultra-Low Volume Applicator For Liquid-Applied Silage Inoculants With That Of A Conventional Applicator, G. Marley, G. Pahlow, H. -H. Herrmann, T. R. Owen

IGC Proceedings (1977-2023)

Liquid-applied silage inoculants are normally sprayed onto forages cut for ensiling at application rates from 1 to 3 l/t. Applicator tanks can require frequent re-filling, especially with large self-propelled forage harvesters having harvest rates in excess of 1000 t/d. This can be an issue for fields remote from the farm, for areas with restricted water availability and for contractors paid by the area harvested. This study was conducted to assess the efficacy of inoculant distribution on the crop using a simple, ultra-low volume (ULV) applicator compared with a conventional liquid-applied silage inoculant applicator.

Improving The Aerobic Stability Of Whole-Crop Cereal Silages, I. Filya, E. Sucu, A. Karabulut

IGC Proceedings (1977-2023)

Whole-crop cereal silages, such as wheat, sorghum, and maize are susceptible to aerobic deterioration, especially in warm climates. This is because aerobic yeasts are the most active at 20-30°C (Ashbell et al., 2002). Therefore, it is very important to find suitable additives that inhibit fungi and protect the silage upon aerobic exposure. Propionibacterium acidipropionici is propionic acid bacteria (PAB), which produce propionic and acetic acid in silage. Results with these micro-organisms in laboratory studies were promising with regard to aerobic stability. The purpose of the present work was to study the effects of PAB, lactic acid bacteria (LAB) and …