Life Sciences Commons^™

Isolation And Characterisation Of Genes Encoding Malate Synthesis And Transport Determinants In The Aluminum-Tolerant Australian Weeping-Grass (Microlaena Stipoides), R. M. Polotnianka, E. Ribarev, L. Mackin, K. A. Sivakumaran, G. D. Nugent, U. P. John, G. C. Spangenberg

IGC Proceedings (1977-2023)

Acid soils cover some 40% of the Earth’s arable land where they represent a major limitation to plant production. Plant growth on acid soils is primarily limited due to aluminium (Al) solubilized by acidity into toxic Al³⁺ cations which will inhibit root growth resulting in poor uptake of water and nutrients. Many important pasture species lack sufficient Al tolerance within their germplasm to allow effective breeding for this character.

Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species, P. Barre, G. Darrieutort, J. Auzanneau, B. Julier

IGC Proceedings (1977-2023)

In many cases, the analysis of the genetic bases of any trait requires molecular markers and if possible co-dominant PCR-based ones. In perennial fodder species, the number of publicly available markers (microsatellites and Sequence Tagged Site (STS)) is limited. Our goal is to use sequences from model grass species, i.e. rice, wheat, maize, barley, in L. perenne in order to develop STS markers in interesting regions such as under a QTL (Quantitative Trait Loci) or around a candidate gene,. As the genome sequence of rice is now available, the objective was to use the sequences of genes included in the …

Application Of Molecular Markers Derived From Medicago Truncatula In White Clover (Trifolium Repens L.), C. Jones, M. T. Abberton

IGC Proceedings (1977-2023)

White clover is the major forage legume of temperate areas. Genome maps have been produced recently (Jones et al., 2003; Barrett et al., 2004) and the location of QTL for important agricultural traits reported (Abberton et al., 2004). White clover is closely related to the model legume Medicago truncatula and there is likely to be considerable benefit in applying genomic resources from model to crop. However, the extent of synteny between the species must be established. Here we present preliminary results detailing progress towards this goal.

Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits, S. C. Thain, P. Morris, S. Hawkins, C. Morris, I. S. Donnison

IGC Proceedings (1977-2023)

Currently the potential of biomass crops, including grasses, is limited because most species have not been bred for this purpose. However traits such as lignification, phenolic cross-linking and carbohydrate accessibility, which are also important for nutritive quality in forage grasses, can affect potential biofuel quality in applications such as combustion, fast-pyrolysis or fermentation. A collection of Lolium and Festuca species known to exhibit a range of lignin, cell wall phenolic and carbohydrate concentrations have been used to test optimum characteristics for biofuel processing. This collection formed a “calibration” set for subsequent high through-put FTIR chemical screening of additional plant lines: …

A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg

IGC Proceedings (1977-2023)

Knowledge of the expression pattern of genes provides a valuable insight into gene function and role in determining the observed heritable phenotype. High–density cDNA and oligonucleotide microarrays represent powerful tools for transcriptome analysis to gain an understanding of gene expression patterns for thousands of genes. Internationally coordinated efforts in transcriptome analyses and sharing of microarray resources will benefit the advancement of our understanding of gene function in forage and turf species.

Effects Of Various Dietary Carbohydrate Sources On The Growth Performance And Body Composition Of The Dog Conch, Laevistrombus Canarium, Jen-Hong Chu

Journal of Marine Science and Technology–Taiwan

This study investigates the effects of dietary supplementation with various carbohydrate sources on the growth and dietary nutrient utilization efficiency of a 0.72-g dog conch, Laevistrombus canarium. Five treatment diets had supplementation with alpha-starch (Sta), dextrin (Dex), pullulan gum (Pg), xanthan gum (Xg), and carboxymethyl cellulose (CMC). At the end of the feeding trial, the best growth performance (weight gain, 442.23%) and diet efficiency (protein efficiency ratio, 1.25, and feed conversion ratio [FCR], 2.04) were exhibited by the dog conch fed the Sta diet, whereas the lowest growth performance was observed in the dog conch fed the Xg and CMC …

Cost Advantages Of Far East/Europe Trunk Route Deployment With Port Selection In East Asia, Tai Hui-Huang, Chang Chin-Wei

Journal of Marine Science and Technology–Taiwan

To cater to the gradually increasing sizes of ships, several traditional container ports in East Asia built deep-water wharves to attract shipping carriers to berth, a decision that is considered highly reasonable because it allows for shipping carriers to gain a cost advantage. For traditional Far East/Europe (F/E) trunk routes, shipping carriers must deploy vessels that are large enough at hub ports to maintain low transshipment costs. However, for a port to attract shipping carriers, it should be able to first meet the cargo demand of these carriers. The port would also need to improve the loading ratio to enjoy …

Influence Of Vacuum Condition On Rapid Chloride Migration (Rcm) Test For Chloride Penetration Resistance Of Concrete, Chih-Yen Lin, Chun-Fu Chang, Chung-Chia Yang

Journal of Marine Science and Technology–Taiwan

NT Build 492 regulates that the pressure be set at 7.5–37.5 torr and be maintained for 3 h for vacuum processing. This large range of acceptable vacuum pressure is awkward for research, and 3 h of pump-down time may be excessive. In this study, vacuum conditions were divided into vacuum pressure and pump-down time groups: various vacuum pressures and pump-down times were used to preprocess specimens to explore the effect of vacuum conditions on the RCM test. The salt ponding test was also conducted to identify the optimal vacuum conditions. It is shown that the relationship between vacuum pressure and …

Exhaust Noise Elimination Using Silencers Fortified With Perforated Tubes, Extended Tubes, Penetrable Resin Inlet And Orifice Plate, Min-Chie Chiu, Ho-Chih Cheng

Journal of Marine Science and Technology–Taiwan

Pneumatic equipment in marine diesel engine and associated piping system often emit extremely high level of noises when releasing pressure. Concerning the crew’s hearing health, discovering an efficient noise silencing device becomes essential. A silencer fortified with extended tubes, perforated tubes, orifice plate, and penetrable resin inlet is presented in order to competently dampen the blown-up noises. A simplified objective function by means of FEM, Artificial Neural Networks (ANNs), and a Genetic Algorithm (GA) is established to enable the numerical calculation when using a finite element method. Three silencer designs, silencer A (with element A, a penetrable resin inlet), B …

Deep Learning–Based, Oceantdlx Sea Ice Detection Model For Sar Image, Liu Lin, Li Wanwu, Li Hang, Sun Yi

Journal of Marine Science and Technology–Taiwan

This study constructs four deep-learning OceanTDLx series models and uses a WinR-AdaGrad gradient descent algorithm to train and optimize the constructed models. Through an analysis of the loss, accuracy, and time consumption of the four models (i.e., OceanTDL2, OceanTDL3, OceanTDL5 and OceanTDL8), we reveal that the models’ performance does not improve when the number of layers is increased and that OceanTDL5 provides the optimal performance. OceanTDL5 is compared with OceanTDA9 (a model that we previously constructed), and the curves for training loss_batch and training accuracy_batch indicate that OceanTDL5 is more suitable than OceanTDA9 for detecting distributed targets, particularly semi-melted sea …

Application Of Unmanned Aerial Vehicle–Based Infrared Images In Determining Characteristics Of Sea Surface Temperature Distribution, Hsing-Yu Wang, Hui-Ming Fang, Yun-Chih Chiang

Journal of Marine Science and Technology–Taiwan

This study investigated the temperature field of a body of water by using rotary-wing unmanned aerial vehicles (DJI Mavic 2 Enterprise Dual) comprising visible light and infrared cameras to determine sea temperature distribution. With a flight height of 150 m, image overlap of 80%, and image resolution of 4.75 cm/pixel, the proposed method was more effective than the conventional portable conductivity–temperature–depth device in rapidly measuring sea surface temperature and determining sea temperature distribution. The discharged heated water of the Hoping Power Plant traveled 100–330 m from the wastewater outfall and then gradually diffused in the range of 100–490 m before …

Exploration Of C9or72c9-500 Sk2, Sk3 Channel Expression, Teresa L. Garrett

Festival of Research

ALS (also known as Lou Gehrig's Disease) is a neurodegenerative disease that weakens muscles and impacts physical function. The C9 Gene is responsible for creating proteins that help neurons send and receive signals across the synapse. Small Conductance calcium activated potassium channels (SK channels) affect the hyperpolarization phase of an action potential. Hypothesis: SK2 and SK3 clusters of C9 positive cells should decrease in size and intensity when exposed to a disease state compared to C9 negative controls.

Pamam- Cyclodextrin Conjugate Upregulates Brain-Derived Neurotropic Factor In Arpe-19 Cells, Gopika Ashokan

USF Tampa Graduate Theses and Dissertations

Background: Traumatic Brain Injury (TBI) is a major contributor to death and disability due to motorvehicle accidents, sports, physical abuse, and battlefield injuries. The primary insult to the brain leads to inflammation, vascular dysfunction, and oxidative stress in the brain as well as in the eye. This leads to loss of Retinal Ganglion Cells (RGCs) and downregulation of Brain derived Neurotopic Factor (BDNF). BDNF is a neurotrophic factor that binds to Tropomyosin Receptor Kinase B (TrkB) receptor to promote cell growth, survival, and differentiation. Current treatment strategies do not promote neuronal regeneration. Therefore, novel treatments are needed to restore vision …

Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics Phase Ii, Jackson Ebbers, Heidi L. Hillhouse, John A. Guretsky, Thomas J. Weissling

Nebraska Department of Transportation: Research Reports

No abstract provided.

Acoustic Telemetry Provides Insights For Improving Conservation And Management At A Spawning Aggregation Site Of The Endangered Nassau Grouper (Epinephelus Striatus), Richard S. Nemeth, Elizabeth Kadison, Jonathan Jossart, Mahmood Shivji, Bradley Wetherbee, Jordan Matley

Biology Faculty Articles

Nassau grouper (Epinephelus striatus, Serranidae) were one of the most important fishery species in the Caribbean, but are now listed as critically endangered. Population collapse in most places occurred primarily by fishers targeting their spawning aggregation sites, where thousands of fish gathered during full moon periods from December to April. Identifying management options for protecting this vulnerable period is crucial for their survival. In the US Virgin Islands, we tagged 24 Nassau grouper with acoustic transmitters at a fish spawning aggregation (FSA) site called the Grammanik Bank, a 1.5 km2, marine protected area (MPA) closed each year from …

Effect Of Uv-C Light Against Listeria Monocytogenes On Hydroponically Grown Lettuce And Its Effect On Quality, Ivannova Lituma

LSU Master's Theses

Controlled agriculture environments like hydroponic systems are a growing industry. However, studies have shown potential of microbial growth in hydroponic systems, highlighting the need for decontamination techniques to prevent the risk of foodborne diseases. This study evaluated the effect of UV-C light against Listeria monocytogenes on hydroponically grown lettuce and seeds. Lettuce leaves were spot inoculated (100μL) with Listeria monocytogenes cocktail and exposed to UV-C light (0.28mW/cm2) for 10 to 50 seconds in 10-second increments and 1 to 5 minutes in 1- minute increments. UV treated leaves were stored at 4C° and visually inspected for 5 days. Additionally, the smooth …

Small Molecule Compounds, Active Ingredients Of Tcm, As Novel Therapeutics For Eosinophilic Esophagitis, Anish Maskey

NYMC Student Theses and Dissertations

Eosinophilic Esophagitis (EoE), a chronic inflammatory condition of the esophagus, characterized by esophageal dysfunction and eosinophilic inflammation. Current treatments for EoE include dietary elimination and swallowed steroids. Recently, Dupixent was approved for the treatment of EoE, but it has side effects such as eye inflammation and eczema. Traditional Chinese Medicine (TCM) practice has been used to improve GI symptoms in EoE patients. Two key active ingredients of a TCM formula, 7,4’-Dihydroxyflavone (DHF) and Berberine (BBR), have shown remarkable results in treating EoE.

To understand the role of two active ingredients in EoE, different experimental models were used. First, a computational …

Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans

Theses and Dissertations

Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during mixing, extrusion, and drying. These data are essential to pasta producers seeking to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in wheat flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in raw semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. This study also assessed the appropriateness of E. faecium NRRL B-2354 as …

Genetic Correlations Between Alzheimer’S Disease And Gut Microbiome Genera, Joan Manuel Cue, Jenifer Do, Jeffrey Ebersole, Xiangning Chen, Edwin C Oh, Jeffrey L Cummings, Jingchun Chen

Faculty, Staff and Student Publications

A growing body of evidence suggests that dysbiosis of the human gut microbiota is associated with neurodegenerative diseases like Alzheimer's disease (AD) via neuroinflammatory processes across the microbiota-gut-brain axis. The gut microbiota affects brain health through the secretion of toxins and short-chain fatty acids, which modulates gut permeability and numerous immune functions. Observational studies indicate that AD patients have reduced microbiome diversity, which could contribute to the pathogenesis of the disease. Uncovering the genetic basis of microbial abundance and its effect on AD could suggest lifestyle changes that may reduce an individual's risk for the disease. Using the largest genome-wide …

Evidence Of Distinct Genetic Stocks Of The Bottlenose Wedgefish (Rhynchobatus Australiae) In The Indo-West Pacific, Rehema J. Simwanza, Cyrus Rumisha

Tanzania Journal of Science

Populations of the bottlenose wedgefish (Rhynchobatus australiae) in the Indo-West Pacific (IWP) have declined by nearly 80% in recent decades. In response, IWP countries are establishing sanctuaries to provide refuge for the fish. However, little is known about the genetic stock structure of the fish in the region. Hence, this study analysed partial sequences (610 base pairs) of the cytochrome oxidase subunit (COI) gene from eight bottlenose wedgefish populations in the IWP to assess the genetic stock structure of the fishery. The sequences revealed that Western Indian Ocean (WIO) populations are genetically distinct from those in the West Pacific (WP) …

Prevalence Of Endoparasites And Haematology In Redbelly Tilapia From A Shallow Tropical Reservoir In Ghana, Elliot H. Alhassan, Samuel A. Osei, Akwasi Ampofo-Yeboah, Samuel O. Dandi

Tanzania Journal of Science

The goal of this study was to assess common endoparasites on Tilapia zillii in the Tono Reservoir, as well as to investigate the mean intensity and prevalence of these parasites. A total of 175 fish specimens were examined during the study. Endoparasites of three metazoans and one protozoan were observed from the Tilapia zillii gills, intestines, and stomach. The findings showed that the most common endoparasites affecting Tilapia zillii are Ichthyopthirius multifiliis, Bothriocephalus sp., Contracaecum sp., and Dactylogyrus sp. Also, Contracaecum sp had the highest prevalence of 29.71, followed by Bothriocephalus sp (18.86), Ichthyopthirius multifiliis (12), and Dactylogyrus sp (6.86). …

Effects Of Vacuum Packaging And Chilling Storage On The Microbiological Changes Of The Superheated Steam Dried Sardines, Prissillah Antelm, Ibrahim Juma, Leonard M.P. Rweyemamu, Lillian Daniel Kaale

Tanzania Journal of Science

Fish are important sources of protein and micronutrients for combating malnutrition. Since fish are highly perishable, methods for processing and preserving them should be the main focus to increase their shelf life and maintain their quality. The present study assessed the effectiveness of the superheated steam dryer (SSD) in processing sardines, and examined the effects of packaging and storage conditions on their microbiological quality. Sardines collected from the Mafia Island were dried in SSD, air-packed and stored at room temperature (AR), air-packed and stored at chilling temperature (AC), vacuum-packed and stored at room temperature (VR), and vacuum-packed and stored at …

Land Cover Change As A Proxy Of Changes In Wildlife Distribution And Abundance In Tarangire-Simanjiro-Lolkisale-Mto Wa Mbu Ecosystem, Thobias Anthony, Nyimvua Shaban, Cuthbert Nahonyo

Tanzania Journal of Science

This study analyzed the land use and land cover (LULC) changes from 2000 to 2020 and examined its influence on wildlife distribution and abundance. LULC from satellite imagery for 2000, 2012 and 2020 were acquired from the United States Geological Survey (USGS). Supervised classification along with a maximum likelihood algorithm was used to classify satellite imagery into eight LULC classes: bareland, woodland, wetland, shrubland, grassland, waterbodies, cropland and built-up land. The results showed that grassland, wetland and woodland had declined, while shrubland, cropland and built-up land increased from 2000 to 2020. Grassland, wetland and woodland decreased from 48.1%, 14.1% and …

Household And Community Factors Affecting Nutritional Status Of Under-Five Children (6-59 Months) In Gairo District Using Composite Index Of Anthropometric Failure, Tausi M. Mohamed, Cornelio N. Nyaruhucha

Tanzania Journal of Science

In Tanzania, where the majority of under-five children are affected, undernutrition is still a significant public health concern. The study aimed at assessing the prevalence of undernutrition and its determinants among under-five children in Gairo District using a Composite Index of Anthropometric Failure (CIAF). A household-based cross-sectional study was employed involving 300 under-five children with their mothers/caregivers in three wards in the Gairo District. Data collection on socio-demographics, dietary diversity, and healthcare factors were collected by interviewer-based semi-structured questionnaires. Anthropometric measurements using standard procedures were performed to collect anthropometric data. Odds ratios with a 95% confidence interval and p-value ≤ …

Polyphenolic Content And Radical Scavenging Activities Of The Peel, Pulp And Seed Of Avocado (Persea Americana Mill.) Grown In Tanzania, Ibrahim Juma, Jan-Eric Englund, Rodomiro Ortiz, Mulatu Geleta, Donatha D. Tibuhwa, Anders S. Carlsson, Agnes Nyomora, Moneim Fatih, Helena Persson Hovmalm

Tanzania Journal of Science

Avocado is a healthy fruit and the consumption is continuously growing worldwide. The fruit contains polyphenolic compounds with antioxidant effects. Globally, research has been devoted to exploring the fruit quality, especially compounds with antioxidant effects, from different avocado-growing sites. However, the fruit quality of the Tanzanian avocado has so far not been investigated. In this study, the contents of polyphenols in peel, pulp and seed of avocados sampled in south-western Tanzania are described. The levels of total polyphenolic and flavonoid contents were measured, and antioxidant activity was evaluated using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The total polyphenolic content was highest in …

Generation Of Electricity By Using Microbial Fuel Cell Prototype Fed By Sewage: Case Study At The University Of Dar Es Salaam, Mahir M Said, Asia A Saad, Geoffrey R John, Aviti T Mushi

Tanzania Journal of Science

The access to electricity is still not reliable in Tanzania. Whilst, there are several locations within the country that have wastewater cites that are not economically used to produce electricity. At the University of Dar es Salaam (UDSM), there are main grid power, and few scattered solar panels. This study was intended to bridge the divide between the current increasing power demands of the UDSM by exploiting not so much used sources, such as electric energy from wastewater. This study was undertaken to develop a Microbial Fuel Cell (MFC) prototype fed by sewage in Dar es Salaam, for electricity generation …

Ethanol Extracts From Selected Tanzanian Medicinal Plants Selectively Inhibit Plasmodium Falciparum Growth In Vitro, Robert Christopher, Amina Msonga, Heinrich C. Hoppe, Fabrice F. Boyom

Tanzania Journal of Science

This study aimed to assess the in vitro antiplasmodial and cytotoxic activities of ethanol extracts from the root barks, leaves and stem barks of seven medicinal plant species growing in Tanzania. The antiplasmodial activities of extracts against chloroquine-sensitive (3D7) and multidrug-resistant (Dd2) strains of Plasmodium falciparum were determined using a SYBR green I-based fluorescence assay, while the cytotoxicity of active extracts was determined against the human HepG2 cell line using a resazurin-based assay. Out of twenty-one extracts screened, two of them, namely, DFRE from Dracaena fragrans root bark (IC50Pf3D7 = 2.4 mg/mL) and EHSE from Erythrina haerdii stem bark (IC50Pf3D7 …

Recruitment Behaviour In An African Meliponine Bee Species (Hymenoptera, Apidae: Meliponini): Understanding Glandular Origin And Pheromonal Components, Bridget O. Bobadoye, Olaniyi T. Alamu, Jennifer A. George-Onaho, John A. Ete, Ayangbade E. Ayandokun, Ifeoluwa S. Agboola, Olubusola T. Adeoye, Popoola J. Adekola

Tanzania Journal of Science

Meliponine bees are speculated to use a variety of communication mechanisms to effectively recruit workers of a colony to collect sufficient amounts of food to nourish the entire nest population. Mechanisms used to convey such information include thoracic vibrations and trophallaxis within the nest; footprint secretions and pheromone marks deposited in the field, or a combination of these signals and cues. There have been numerous discrepancies about the origin of trail pheromone production from the head, thorax, abdomen and leg regions of meliponine bees. Because the glandular origin of pheromone marks deposited by African meliponine bee’s species has not yet …

Performa Diagnostik Global Leadership Initiative On Malnutrition (Glim) Dibandingkan Dengan Malnutrition Nutritional Assessment (Mna) Dalam Pengkajian Malnutrisi Pada Pasien Geriatri Di Rawat Inap, Astrine Permata Leoni, Wita Rizki Amelia, Purwita Wijaya Laksmi, Ahmad Syauqy

Jurnal Penyakit Dalam Indonesia

Introduction. As many as 76.6% of patients aged 60 years and above (elderly) are malnourished or at risk of malnutrition based on MNA within the first 48 hours of hospital admission. The prevalence of malnutrition varies widely depending on the population studied, the healthcare setting and the tools used for the assessment. To date, SGA is widely used in the diagnosis of malnutrition for adults and MNA for elderly patients. Recently, experts proposed empirical consensus of GLIM criteria for determining the diagnosis of malnutrition in adults. This study aimed to investigate the diagnostic performance of GLIM criteria as a …

Louisiana Resident Perceptions On Invasive Aquatic Plant Species And Their Management, Anna Ribbeck

LSU Master's Theses

Determining the public perception of invasive species in Louisiana is an important tool to help shape and develop future management decisions, educational material and ultimately public policy. Therefore, the primary purpose of this study was to determine the perceptions of adult Louisiana residents regarding invasive aquatic plant species and their management. An online survey was used to collect data on registered Louisiana residential boaters’ perceptions towards invasive aquatic plants and their management. A postcard with a quick response code leading to the survey was mailed to 6,000 randomly selected Louisiana registered residential boaters. Of the 230 Louisiana registered boaters that …