Life Sciences Commons^™

Management Plan For The Western Painted Turtle At The Sandy River Delta In Troutdale, Oregon, Emma Scott

Environmental Science and Management Professional Master's Project Reports

This management plan has been prepared for the Lower Columbia Estuary Partnership and is intended to provide guidance for the improvement and protection of western painted turtle (Chrysemys picta bellii) habitat at the Sandy River Delta in Troutdale, Oregon. The Sandy River Delta is a 1500-acre natural area situated where the Columbia and Sandy Rivers meet. Its dynamic floodplain habitat supports a diverse assemblage of species, including the western painted turtle, an Oregon Conservation Strategy Species. A small, unknown number of western painted turtles utilize the wetland habitats of the Sandy River Delta, and the frequency in observations …

An Examination Of The Current Knowledge Of Contaminants In Mangroves: Hawaii And Globally, Geoffrey Szafranski

Environmental Science and Management Professional Master's Project Reports

The geographic range of mangrove forests is shifting quickly as they expand poleward in response to climate change while simultaneously being removed from their native extent to clear space for anthropogenic land-uses. Mangrove forests are also known to be sinks for anthropogenic contamination. Yet contamination research is under-researched in mangrove ecosystems, specifically the environmental fate, effect on biodiversity, and risk to human populations from contamination in the context of these changing conditions requires further research. The goal of this thesis is to address this data gap through analysis of contamination in the literature and through an investigational survey of mangrove …

Regenerative Farming Practices: How Much Carbon Do They Sequester?, Andrew M. Havens

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Along with the recent rise of voluntary carbon markets comes potential carbon credit producers seeking reliable information on how much carbon they can expect to sequester. In this thesis a distribution of expected sequestration outcomes is constructed using cost-benefit analysis and data gathered from agronomic experiments and land-grant university crop budgets for cover crops and no-till practices. The inverse cumulative distribution of carbon sequestration outcomes from adopting a regenerative agricultural practice is visualized and the net social benefit of paying farmers to produce carbon credits is estimated. Results show that on average there is between $29.02 and $37.20 of social …

Intergenerational Change In Hiv/Aids-Related Stigma In Cato Manor, Mijin Cho

Independent Study Project (ISP) Collection

In South Africa, the world’s epicenter of the HIV/AIDS pandemic, HIV-related stigma and stigma-enforcing stereotypes continue to serve as major health barriers to receiving adequate HIV prevention and treatment. While there continues to be a rise in research and advocacy, there is a need to study HIV stigma through a multigenerational lens that addresses the changing HIV/AIDS stigma in the post-apartheid era. Using qualitative methodology, this study aims to explore the generational differences in perceived HIV stigma between females in their 20s and above 50 years of age (as denoted by “50s+”) living in Cato Manor, South Africa. The study …

Mycorrhizal Fungi And Reforestation In An Eastern Lowland Rainforest Of Madagascar, Sophia Gibby

Independent Study Project (ISP) Collection

Most terrestrial plants worldwide make associations with mycorrhizal fungi, yet the fungal diversity of Madagascar is vastly unknown. This research project is a preliminary investigation into the mycorrhizal status of 19 tree species in and near Ranomafana National Park (RNP), a lowland tropical forest on the southeast coast of Madagascar. Arbuscular mycorrhizal (AM) colonization in these species was studied in root samples under the microscope in the laboratory of Centre ValBio (CVB). The degree of AM infection was assessed for three study areas: one directly adjacent to a protected area and two reforestation sites supported by CVB. For the 19 …

Tropical Tree Carbon Storage At Drago Dos Forest In Boca Del Drago, Panama, Ben Dwyer

Independent Study Project (ISP) Collection

Currently, anthropogenic carbon emissions pose a significant, global threat, contributing to Global Warming and Climate Change (CC). Today, the most effective carbon sinks are natural. Trees are highly effective carbon sinks that sequester large amounts of atmospheric carbon dioxide (CO2), most greatly in tropical areas. However, tropical tree carbon storage needs to be more accurately estimated to provide valuable information toward mitigating CC and its negative environmental effects. This study aimed to estimate CO2 sequestration at a tropical forest in Boca Del Drago, Panama, and compare it to that of a nearby mangrove forest. It was hypothesized that the tropical …

Anuran Species Diversity And Abundance Between Three Locations In El Valle De Antón, Coclé, Panamá, Xena Gehring

Independent Study Project (ISP) Collection

Panama is an incredibly biodiverse country that houses 230 species of amphibians including 188 species of frogs, 35 of which are endemic to Panama. Commonly found in terrestrial and aquatic habitats, frogs play a very important role in the regulation of insect populations and serve as a valuable food resource to other predators. The greatest threat to amphibian species at this time is Chytridiomycosis, a fungal pathogen that infects the skin and often results in death. Many species of amphibians have already gone completely extinct or are extinct in the wild due to the proliferation of this disease. The skin …

Agroforestry For The Future: Motivations Behind Tasmanian Farmers Planting Trees, Josh Lipp

Independent Study Project (ISP) Collection

Agroforestry is the act of combining farming and tree planting, and many Tasmanian farmers are starting to plant trees on their properties for multiple reasons. Through conducting 9 interviews with farmers and researchers and visiting field sites, 3 main themes were identified to answer the question: why are Tasmanian farmers planting trees, and what are the benefits and downsides to agroforestry? Interviews help us understand farmers’ perceptions of agroforestry, and field data collection will prove the benefits of agroforestry in the future. Tasmania was chosen as the location of study as it is a state in Australia that largely focuses …

Spatial Ecology And Habitat Preference Of Yellow-Spotted Monitors (Varanus Panoptes) At Lizard Island National Park, Qld, Australia, Ryan Snyder

Independent Study Project (ISP) Collection

Lizard Island National Park is an area of strong habitat diversity, especially given its small size. There are several distinct habitats represented, including open sclerophyll woodlands, mangrove swamps, dune grasslands, foothills elevations, and of course anthropogenically modified human habitats. The object of this study was to observe the abundances and behaviors of the yellow-spotted monitor lizard (V. panoptes) in each of these habitat settings to answer the question of which habitat type is preferred amongst V. panoptes in the landscape context of Lizard Island National Park.

Study transects were conducted over the course of one month measuring abundance of V. …

Impact Of Wildlife Provisioning On Species Diversity, Relative Frequency, And Richness In New South Wales, Alanah Cohen-Tigör

Independent Study Project (ISP) Collection

Wildlife provisioning and camera trapping are two recently evolving methods of conservation and wildlife management, aimed at protecting animals in the face of ever worsening climate change. Habitats are being destroyed by climate disruption, decreasing species diversity and abundance worldwide. It is imperative that methods of protecting species are developed to slow or reverse this era of extinction. By providing supplementary food and water to ecological communities, the hope is that species will have improved survivorship and reproduction, making them more resilient and resistant to population decline. Camera trapping is product of modern technology, allowing researchers to monitor species without …

Dairy & Development: An Investigation Into The Economic And Sociopolitical Dynamics Of Yak Cheesemaking In Nepal, Audrey Peiker

Independent Study Project (ISP) Collection

Cheesemaking in Nepal is full of love, passion, and creativity. What began as a Swiss development project in the 1950s has now grown into a full-fledged industry, providing jobs, creative outlets, and cultural expression for many. Nepal’s Dairy Development Corporation owns several factories throughout the country, setting the tone for the industry by establishing the standard price for milk and process for cheesemaking. From there, privately held factories innovate and compete, bringing new products to the market and increasing wages, but also struggle with consistency and reliability. Now, Nepal’s cheesemaking market is at a turning point—much of the herding population …

Talking To The Earth: A Digital Documentation Of Biodiversity In The Sherpa Language, Dvij Sharma

Independent Study Project (ISP) Collection

Identified as a pressing matter by organizations as prominent as the UN, the conservation of minority and indigenous languages “is essential for ensuring the continuation and transmission of culture, customs, and history, but it is also important to address biodiversity loss and climate change.” This project focuses on one of the latter issues, biodiversity loss, as it relates to Sherpa, a minority language spoken primarily in Eastern Nepal. In an effort to help preserve the native vocabulary and descriptions of basic plants and animals found in Sherpa-speaking regions, primarily interview-based fieldwork was conducted in the lower Solu regions of Lumsa, …

Status Of Pangolins: A Case Study On "The Most Trafficked Mammal In The World" In Central-South Of Nepal, Tsogyal Wangmo Lama

Independent Study Project (ISP) Collection

Pangolins are subject to constant trafficking in Asia and increasingly in Africa for their meat and scales. Very little research is done on this species due to its nocturnal, burrowing, and elusive nature. The larger the size of an animal is, the better it is known to the people and vice versa. Hence all these eight species of pangolins despite being under the International Union for Conservation of Nature (IUCN) red list with the four Asian species declared endangered or critically endangered, are still illegally abused and used for ulterior motives due to a lack of awareness among the general …

Biodiversidad De Plantas En La Reserva Natural Urbana Punta Popper En Río Grande: Una Propuesta Para Senderos Para La Gestión De La Conservación Y Métodos De Campo Replicables En Un Etorno Educativo, Lili Weir

Independent Study Project (ISP) Collection

The Patagonian Steppe is an ecoregion that is rich in plant biodiversity (both native and introduced), and the Urban Nature Reserves like Punta Popper in Río Grande provide an opportunity to preserve these sensitive species and educate the public, especially students, about the value of this trophic level. However, many beautiful species are being affected by human activities in the Reserve: such as the mismanagement of the trail systems which has led to erosion of the sand dunes, and the pressures of rapid urban expansion contributing to trash pollution. It is essential that stakeholder communities have access to information and …

Understory Epiphyte Hydrology: Analyzing Water Storage Capacity Of Epiphytes Along An Elevational Gradient In Western Ecuadorian Cloud Forest, Angelina Dodge

Independent Study Project (ISP) Collection

Epiphytes are defined as plants that grow on a host, often another plant, and acquire their nutrients and water from the atmosphere. As such is true, epiphyte composition is largely dependent upon atmospheric and thus climactic conditions. Due to high levels of atmospheric water availability that results from the frequent presence of mist, clouds, and high humidity, epiphytic plants grow and thus store water within the above ground region of the Andean cloud forest at levels higher than in any other ecosystem found in Ecuador. This study was done in hopes of revealing any trends of epiphytic water storage capacity …

Hydropower To The People: Implications Of A Comparative Macroinvertebrate Study On Either Side Of The Central Hidroeléctrica Topo In Tungurahua, Ecuador, Grace Mazur

Independent Study Project (ISP) Collection

This study uses macroinvertebrates as bioindicators to assess the water quality upstream and downstream of a hydroelectric project in Ecuador’s eastern cloud forest. Ecuador has increasingly turned to hydropower to supply its energy needs as pressure mounts to turn away from fossil fuels. This transition has been realized on the Río Topo, located in the province of Tungurahua. This study aims to determine how the Central Hidroeléctrica Topo (CHT), a diversion hydroelectric project (HEP) constructed on the Río Topo in the 2010s, has affected the water quality of the river. Samples were taken of benthic (bottom-dwelling) macroinvertebrates in the Río …

The Possible Role For Basigin Receptor Function In The Induction Of Matrix Metalloproteinase Expression In Human Endometrial Stromal Cells, Amy Abel

All NMU Master's Theses

10-15% of couples worldwide are affected by infertility. The leading cause of infertility is implantation failure. Molecular communication in precise time and space between an implanting embryo and a receptive uterus is essential for successful implantation. Basigin has been shown to have vital functions within the implantation process. Basigin is best known for inducing the expression of matrix metalloproteinases. Basigin has been shown to induce MMP expression in vitro in human endometrial stromal cell lines (HESC). Understanding how basigin induces matrix metalloproteinases within normal cellular conditions will help researchers understand how these signaling cascades flow in the framework of implantation. …

The Status Of Invasive Dusky Slug (Arion) Species In The Upper Great Lakes: A Molecular Approach, Olivia W. Hall

All NMU Master's Theses

I update the distribution of two cryptic slug species, Arion fuscus and Arion subfuscus, in the Upper Great Lakes of the United States using molecular identification methods. Arion fuscus has not previously been reported in the literature in this region, and Arion subfuscus has previously been reported as abundant. However, all previous distribution studies were determined using visual identification tools, which can result in misidentification between cryptic species. To molecularly re-examine these distribution maps, I used a mitochondrial ribosomal subunit 16S PCR amplification and subsequent SSpI and MfeI restriction enzyme digest to identify slug specimens. I collected slugs in …

Targeted Mapping Of Oryza Sativa Chilling Tolerance Candidate Genes Using Multiple Stress-Relevant Quantitative Traits, Naoki Simon Shimoyama

Dissertations (1934 -)

Developing chilling tolerant accessions of domesticated Asian rice is a potential source of significant crop improvement to address the needs of a growing global population. The uniquely chilling sensitive nature of the tropically originating Oryza sativa make it the most important staple crop that could gain the maximum benefit from improved tolerance to low temperature stress. However, mechanisms underlying this complex trait are not fully understood. Oryza sativa has two major varietal groups with different levels of chilling tolerance, JAPONICA and INDICA, providing an ideal tool to investigate mechanistic differences in the chilling stress tolerance responses within this important crop …

Neuronal Glutamate Transporters Modulate Reward-Based Behaviors In Mice, Corey Nilon

Biological Sciences

Obsessive-compulsive disorder (OCD) is a neuropsychiatric disorder characterized by uncontrollable, intrusive thoughts (obsessions), restlessness, and repetitive behaviors (compulsions). One of the genes associated with OCD encodes the neuronal glutamate transporter EAAC1. EAAC1 is expressed in the striatum, the main input nucleus of the basal ganglia, implicated with reward, which shows patterns of hyperactivity in patients with OCD. What remains unknown is how EAAC1 contributes to the different behavioral abnormalities of OCD. To address this question, we performed a set of behavioral tests in EAAC1-/- mice. Specifically, we asked how the execution of repetitive reward-based behaviors changed in these mice. Our …

A Review Of The Forensic Applications For The Analysis Of Biological Fluids Using Fluorescence Spectroscopy, Raghavi Patel

Biological Sciences

The use of science to compile information to assist in solving crimes is known as forensic science. Numerous bodily fluids, such as blood, semen, saliva, and vaginal fluid, are frequently discovered at crime scenes. This makes it crucial for forensic scientists to use analytical methods to examine these fluids to learn more about the crime, including the offender, victim, time since deposition, and other details. Although there are many techniques, the focus of this paper is fluorescence spectroscopy. Fluorescence spectroscopy is a rapid, inexpensive, and non-destructive technique used for forensic examination (Weber & Lednev, 2022). Fluorescence spectroscopy can be used …

Evidence That The Inner Nuclear Membrane Is A Functional Β1-Adrenergic Receptor Signaling Platform In Astrocytes, Kelsey Benton

Dissertations (1934 -)

The noradrenergic system exerts powerful modulatory influences over fundamental brain processes including attention, arousal, learning, and memory by activating adrenergic receptors on neuronal and glial cells. In addition to triggering rapid, transient changes in cellular physiology that drive short-term behavioral actions, these G-protein-coupled receptors also initiate delayed, long-lasting changes in cellular function by regulating gene expression. For example, in astrocytes, norepinephrine-induced activation of β-adrenergic receptors (β-ARs) rapidly induces glucose mobilization from glycogen stores, while simultaneously initiating delayed changes in the expression of glycogen storage genes. Regulation of gene expression requires adrenergic receptor-initiated intracellular signals to reach the nuclear compartment. To …

Altered Motivation & Dopamine Subcircuit Plasticity Unique To Sex During Protracted Opioid Withdrawal, Devan Marc Gomez

Dissertations (1934 -)

Opioid use disorder (OUD) is associated with enduring psychological withdrawal symptoms believed to contribute to drug abuse. Amongst these are shifts in motivational states, wherein pursuit of drug consumption exceeds that of non-drug rewards, reinforcing escalated opioid use and relapse vulnerability. A critical regulator of behavioral reinforcement, the mesoaccumbal dopamine system is thought to be both necessary and sufficient for opioid motivation. However, previous research into its involvement in opioid withdrawal has been limited to acute rather than protracted timepoints, global neuroadaptations rather than those in subcircuits, and overwhelmingly focused on males over females. Using a rodent model of self-administration …

Postnatal Developmental Trends In Membrane Excitability And Bk Channel Function In The Rodent Hippocampus, Michael S. Hunsberger

Dissertations (1934 -)

This dissertation outlines the postnatal development of excitability as well as expression and function of BK potassium channels in hippocampal neurons. I used patch clamp electrophysiology to measure how neuronal action potential waveforms and action potential firing frequencies change in early development, and how pharmacological blockade of BK channels affects these properties in hippocampal neurons. I also describe how the protein expression of the BK channel pore-forming α subunit and mRNA expression of different variants of the pore forming α subunit and auxiliary beta-4 subunit changes with development. I demonstrate in both cultured rat hippocampal neurons across the first seven …

Energy Expenditure And Availability In Active Individuals With Physical Disabilities, Scott Richmond, Amanda Bayer, Chad Kerksick, Heather Pennington, Petey Mumford, Annie Alameda

Faculty Scholarship

The purpose of this study was to evaluate the risk of low energy availability (LEA) in a population of active individuals with physical disabilities. Healthy, active males and females with a physical disability (n=13; Mean±SD; Age: 26.4±6.5 years; Height: 143.4±59.6 cm; Body mass: 57.5±27.3 kg; Fat-free mass: 41±20.8 kg;) were recruited to participate in this study. Participants were expected to track their exercise energy expenditure (EEE) and energy intake (EI) over the course of five days using portable wrist and chest-strap heart rate monitors. Body composition assessment completed via dual-energy x-ray absorptiometry (DXA) and resting energy expenditure (REE) performed via …

A Kinome-Wide Crispr Screen Identifies Ck1Α As A Target To Overcome Enzalutamide Resistance Of Prostate Cancer, Jinghui Liu, Yue Zhao, Daheng He, Katelyn M. Jones, Shan Tang, Derek B. Allison, Yanquan Zhang, Jing Chen, Qiongsi Zhang, Xinyi Wang, Chaohao Li, Chi Wang, Lang Li, Xiaoqi Liu

Markey Cancer Center Faculty Publications

Enzalutamide (ENZA), a second-generation androgen receptor antagonist, has significantly increased progression-free and overall survival of patients with metastatic prostate cancer (PCa). However, resistance remains a prominent obstacle in treatment. Utilizing a kinome-wide CRISPR-Cas9 knockout screen, we identified casein kinase 1a (CK1a) as a therapeutic target to overcome ENZA resistance. Depletion or pharmacologic inhibition of CK1a enhanced ENZA efficacy in ENZA-resistant cells and patient-derived xenografts. Mechanistically, CK1a phosphorylates the serine residue S1270 and modulates the protein abundance of ataxia telangiectasia mutated (ATM), a primary initiator of DNA double-strand break (DSB)-response signaling, which is compromised in ENZA-resistant cells and patients. Inhibition of …

Identification Of A Nacc1-Regulated Gene Signature Implicated In The Features Of Triple-Negative Breast Cancer, Chrispus Ngule, Hami Hemati, Xingcong Ren, Oluwafunminiyi Obaleye, Amos O. Akinyemi, Felix Oyelami, Xiaofang Xiong, Jianxun Song, Xia Liu, Jin-Ming Yang

Markey Cancer Center Faculty Publications

Triple-negative breast cancer (TNBC), characterized by a deficiency in estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor2 (HER2), is among the most lethal subtypes of breast cancer (BC). Nevertheless, the molecular determinants that contribute to its malignant phenotypes such as tumor heterogeneity and therapy resistance, remain elusive. In this study, we sought to identify the stemness-associated genes involved in TNBC progression. Using bioinformatics approaches, we found 55 up- and 9 downregulated genes in TNBC. Out of the 55 upregulated genes, a 5 gene-signature (CDK1, EZH2, CCNB1, CCNA2, and AURKA) involved in cell regeneration was positively correlated …

Leukocyte Tyrosine Kinase (Ltk) Is The Mendelian Determinant Of The Axolotl Melanoid Color Variant, Mirindi Kabangu, Raissa Cecil, Lloyd Strohl Ii, Nataliya Y. Timoshevskaya, Jeramiah James Smith, Stephen Randal Voss

Markey Cancer Center Faculty Publications

The great diversity of color patterns observed among amphibians is largely explained by the differentiation of relatively few pigment cell types during development. Mexican axolotls present a variety of color phenotypes that span the continuum from leucistic to highly melanistic. The melanoid axolotl is a Mendelian variant characterized by large numbers of melanophores, proportionally fewer xanthophores, and no iridophores. Early studies of melanoid were influential in developing the single-origin hypothesis of pigment cell development, wherein it has been proposed that all three pigment cell types derive from a common progenitor cell, with pigment metabolites playing potential roles in directing the …

In Situ Microwave Fixation Provides An Instantaneous Snapshot Of The Brain Metabolome, Jelena A. Juras, Madison B. Webb, Lyndsay E. A. Young, Kia H. Markussen, Tara R. Hawkinson, Michael D. Buoncristiani, Kayli E. Bolton, Peyton T. Coburn, Meredith I. Williams, Lisa P. Y. Sun, William C. Sanders, Ronald C. Bruntz, Lindsey R. Conroy, Chi Wang, Matthew S. Gentry, Bret N. Smith, Ramon C. Sun

Markey Cancer Center Faculty Publications

Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection and preservation by liquid nitrogen. In contrast, we show that these post- mortem changes are not observed with simultaneous animal sacrifice and in situ fixation with focused, high- power microwave. We further employ microwave fixation to define brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple brain regions, evidenced by …

Targeting Lncrna Ddit4-As1 Sensitizes Triple Negative Breast Cancer To Chemotherapy Via Suppressing Of Autophagy, Ting Jiang, Jiaojiao Zhu, Shilong Jiang, Zonglin Chen, Ping Xu, Rong Gong, Changxin Zhong, Yueying Cheng, Xinyuan Sun, Wenjun Yi, Jinming Yang, Wenhu Zhou, Yan Cheng

Markey Cancer Center Faculty Publications

In this study, it is found that the lncRNA, DNA damage inducible transcript 4 antisense RNA1 (DDIT4-AS1), is highly expressed in triple-negative breast cancer (TNBC) cell lines and tissues due to H3K27 acetylation in the promoter region, and promotes the proliferation, migration, and invasion of TNBC cells via activating autophagy. Mechanistically, it is shown that DDIT4-AS1 induces autophagy by stabilizing DDIT4 mRNA via recruiting the RNA binding protein AUF1 and promoting the interaction between DDIT4 mRNA and AUF1, thereby inhibiting mTOR signaling pathway. Furthermore, silencing of DDIT4-AS1 enhances the sensitivity of TNBC cells to chemotherapeutic agents such as paclitaxel both …