Life Sciences Commons^™

Pattern Dynamics And Stochasticity Of The Brain Rhythms, Clarissa Hoffman, Jingheng Cheng, Daoyun Ji, Yuri Dabaghian

Faculty, Staff and Student Publications

Our current understanding of brain rhythms is based on quantifying their instantaneous or time-averaged characteristics. What remains unexplored is the actual structure of the waves-their shapes and patterns over finite timescales. Here, we study brain wave patterning in different physiological contexts using two independent approaches: The first is based on quantifying stochasticity relative to the underlying mean behavior, and the second assesses "orderliness" of the waves' features. The corresponding measures capture the waves' characteristics and abnormal behaviors, such as atypical periodicity or excessive clustering, and demonstrate coupling between the patterns' dynamics and the animal's location, speed, and acceleration. Specifically, we …

Evaluating Form And Function Of Groundcovers And Their Environmental Impacts In Louisiana Landscapes, Thomas Maxwell Mckeown

LSU Master's Theses

Increasing environmental concerns are encouraging the adoption of sustainable landscapes that have environmental, social, and financial benefits. Ornamental groundcover systems are touted as sustainable landscape features due to the associated decreased demand of water, fertilizer, pesticide, and maintenance; however, limited research exists on soil property effects, planting density, weed density, or consumer preferences of groundcovers. This study was aimed to gain a more thorough understanding of ornamental groundcovers systems and their environmental impacts. The effects of groundcover growth habit (matting; bunching) and irrigation delivery (micro spray; overhead) on soil temperature, volumetric water content (VWC), and electric conductivity (EC) along with …

Gulf Sturgeon Mesohabitat Use And Movement In The Lower Pearl River Louisiana-Mississippi, Amanda Popovich

LSU Master's Theses

After spring migrations to natal rivers Gulf Sturgeon Acipenser oxyrinchus desotoi reside in freshwater holding areas prior to fall emigrations to nearby estuaries. Limited published information on the physical habitat characteristics of these holding areas is available, especially for juvenile fish in the western portion of the Gulf Sturgeon’s distribution. To further investigate freshwater occupancy and movement patterns, 32 acoustic receivers were deployed from late April to October of 2021 and 2022 in 1600-m reaches distributed throughout the lower Pearl River, Louisiana-Mississippi, the westernmost river within their distribution. Generalized linear mixed models and multi-state models were used to determine what …

Leaf Area Index And Light Interception In African Violets (Streptocarpus Sect. Saintpaulia), Chintakunta Keerthi Reddy, Venkat Sai Chatla

Scholars Week

LEAF AREA INDEX AND LIGHT INTERCEPTION IN AFRICAN VIOLETS

(Streptocarpus sect. Saintpaulia)

ABSTRACT Chintakunta Keerthi Reddy, Venkat Sai Chatla Hutson School of Agriculture Mentor: Dr. Megan Taylor

African violets are a genus of six flowering plants from tropical eastern Africa, commonly known as Saintpaulias. They can thrive indoors in low light conditions and bloom all year round. They require less light than other blooming plants and can bloom during regular daylight hours in the US and Canada if given proper exposure. Several light intensities were used for the African violet (Streptocarpus sect. Saintpaulia) experiment, which showed a significant variation in …

Effects Of Salinity, Alkalinity, And Acidity On Crop Growth And Development On Pea (Pisum Sativum), Laxmi Prasanna Shakamudi, Rasagna Chamakura, Vineela Bathula

Scholars Week

Effects of salinity, alkalinity, and acidity on crop growth and development

on pea (Pisum sativum)

Authors: Laxmi Prasanna Shakamudi, Rasagna Chamakura, Vineela Bathula

Mentor: Dr. Megan Taylor

Hutson school of agriculture, Murray state university

Pea (Pisum sativum) is one of the vegetables that belong to the Fabaceae family, is a pulse crop that has been widely used and cultivated in all parts of the world. As with many crops pea plants also experience abiotic stresses that affect the plant’s growth, development, and productivity. Abiotic stresses that affect crop growth and development include soil salinity, alkalinity, and …

Effects Of Planting Depth Of Soybean (Glycine Max) On Growth And Development, Hannah Dejournett, Mallory White, Mason Welden

Scholars Week

Effects of Planting Depth of Soybean (Glycine max) on Growth and Development

Hutson School of Agriculture, Murray, KY

Mentor: Dr. Megan Taylor

Hannah DeJournett, Mallory White, Mason Welden

The objective of this study was to examine the effects of soybean (Glycine max) seed placement on growth and development. Several abiotic and biotic factors influence germination and emergence, planting depth is no exception. Planting depth is a management practice that can be controlled at the time of planting, making it an ideal candidate for experimentation. To determine the effect of planting depth on soybeans, soybeans were established at different depths. Previous …

Lai As Affected By Flashing-Light Interception With Corn And Soybeans, Josh W. Pohl, Jondavid Mcclanahan, Jake Eaker

Scholars Week

LAI as Affected by Flashing-Light Interception With

Corn (Zea mays) and Soybeans (Glycine max)

Corn (Zea mays) and Soybean (Glycine max) are both staple crops in global agriculture. With growing concern regarding food security and production, indoor cultivation of corn and soybean plants has become a question of interest. This study looks at the application of different lighting regimes for corn and soybeans and effects on development. Light that can be used by plants is called photosynthetically active radiation (PAR). PAR is the solar radiation range of energy that plants use for …

Soil Properties Associated With Land Management Practices In Western Kentucky, Ava Isaacs, Gracey Moffitt

Scholars Week

Soil Properties Associated with Land Management Practices in Western Kentucky

1. A. Isaacs, G. Moffitt, W. Sides, and Corey Hale

Mentor: Dr. I.P. Handayani

Hutson School of Agriculture, Murray State University, Kentucky, USA

Abstract

One of the most important factors regarding soil and plant health is nutrient availability and fertility. Of these two factors that were mentioned available potassium is a key component. With that being said we decided that it would be beneficial to see how potassium can be traced within the soil in various locations after fertilizer is applied. There were two parts to this experiment. Part one was …

Effect Of Seed Storage On Germination Of Wheat (Triticum Aestivum L.), Corn (Zea Mays), And Soybean (Glycine Max), Sangam Dangal, Disha Ande, Harshaditya Bijapuri

Scholars Week

Effect of Seed Storage on Germination of Wheat (Triticum aestivum L.), Corn (Zea mays), and Soybean (Glycine max)

Disha Ande, Harshaditya Bijapuri, Sangam Dangal, Dr. Megan Taylor

Hutson School of Agriculture, Murray State University, Murray, Kentucky

[email protected], [email protected], [email protected]

Seed germination is an essential stage in the plant cycle for growth and development, resulting in the yield's quantity and quality. It is a complex process that is influenced by various biotic and abiotic factors. The storage of seeds leading up to planting is an essential management practice of crop production, as it directly …

Effects Of Soil Salinity, Alkaline Conditions And Acidity On Crop Growth And Development On Radish (Raphanus Sativus), Anvesh Kumar Dasarla, Chandan Prasanna Lakshmi Kumar Sunkavalli, Turki Alanazi

Scholars Week

Effects of soil salinity, alkaline conditions and acidity on crop growth and development on Radish (Raphanus sativus)

Authors:Anveesh Kumar Dasarla,ChandanPrasanna Lakshmi Kumar Sunkavalli,TurkiAlanazi.

Hutson School of Agriculture

Murray State University

Radish (Raphanus sativus) a member of the mustard family, it is a root veggie. This plant, which can be perennial root. The radish is a vegetable that was probably domesticated in Asia or the Mediterranean region. The veggie, which has few calories, is typically consumed raw, though the young leaves can be prepared similarly to spinach. There are numerous radish varieties, one of which is …

Silver Carp Hypophthalmichthys Molitrix Movements In The Lower Cumberland And Lower Tennessee River, Miranda Belanger

Scholars Week

Silver Carp Hypophthalmichthys molitrix are an invasive species making up a large portion of the fish biomass in the lower portions of the Tennessee River and the Cumberland River. This species is expanding its range throughout the upper portions of these rivers, beginning with Kentucky Lake and Lake Barkley. Silver Carp rarely spawn in Kentucky Lake and Lake Barkley, but they can enter these reservoirs through the lock chamber. Thus, the bottleneck created by lock chambers can be used to control expansion of invasive Silver Carp. Although Silver Carp movement patterns have been studied in other portions of the Tennessee …

Adaptive Plasticity Of Coloration In Response To Environmental Change, Karissa Coffield

Scholars Week

When rapid environmental changes occur, different selective forces can create phenotypic trade-offs in which a trait can provide fitness benefits or costs under different environmental conditions. Amphibians are particularly vulnerable to environmental change, and previous research has revealed that some species will plastically respond to variation in temperature and ultra-violet radiation (UVR) by altering their coloration. Divergent selection on coloration may change with elevation and climate induced shifts in temperature because high temperatures are likely to result in lighter color morphs but as elevation increases, UVR exposure increases leading to the prediction that darker color morphs will be more common. …

Reactive Chlorine Species Reversibly Inhibit Dnab Protein Splicing In Mycobacteria, Daniel Wahl

Scholars Week

Intervening proteins, or inteins, are mobile genetic elements translated within host polypeptides and removed at the protein level by splicing. In protein splicing, a selfmediated reaction removes the intein, leaving only a peptide bond in place. While protein splicing can proceed in the absence of external co-factors, several natural examples of conditional protein splicing (CPS) have emerged. In CPS, the rate and accuracy of splicing is highly-dependent on environmental conditions. As activity of the intein-containing host protein is compromised prior to splicing, and inteins are highly abundant in the microbial world, CPS represents an emerging form of …

Do You Text & Walk? What's The Risk?, Makena Yelland, Quinlan Manross, Abraham Silva, Tyler Block, Fabio Fontana, Hyeyoung Cho

INSPIRE Student Research and Engagement Conference

The purpose of the research is to capture the recognition of gaze fixation, gait behaviors, and texting performance amongst young adults.

Gene Discovery And Molecular Dissection Of Lignin Biosynthesis In Perennial Ryegrass (Lolium Perenne), A. Lidgett, M. Emmerling, R. Heath, R. Mcinnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E. K. Ong, K. F. Smith, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1977-2023)

Lignification of plant cell walls has been identified as a major factor limiting forage digestibility. It limits the amount of digestible energy available to livestock, resulting in an incomplete utilisation of cellulose and hemicellulose by ruminant animals. Modification of the lignin profile of ryegrasses (Lolium spp.) and fescues (Festuca spp.) is undertaken through modulating the expression of genes encoding enzymes involved in the biosynthesis of monolignols.

Identification Of Putative AtTt2 R2r3-Myb Transcription Factor Orthologues In Tanniferous Tissues Of L. Corniculatus Var. Japonicus Cv Gifu, D. N. Bryant, P. Bailey, P. Morris, Mark P. Robbins, C. Martin, T. Wang

IGC Proceedings (1977-2023)

R2R3-MYB plant transcription factors are sequence–specific DNA-binding proteins, which regulate the expression of specific gene(s) following the R2R3 DNA-binding domain interacting with the corresponding promoter sequence(s). The biosynthetic pathway leading to the production of anthocyanins has been demonstrated to be under MYB transcriptional regulatory control (Cone et al., 1986), while the accumulation of proanthocyanidins (PAs) in Arabidopsis seed coats is determined by the R2R3-MYB AtTT2 (Nesi et al., 2001). Using an informatics approach, partial sequences of putative AtTT2 orthologues have been identified and cloned from the forage legume Lotus corniculatus var. japonicus cv Gifu.

Role Of Microorganisms In Communication Between Soil And Plants, Domenico Prisa

Karbala International Journal of Modern Science

Microbial population in the rhizosphere establishes a number of important interactions with plants, whose study is crucial in perspective of sustainable agricultural production. Studies on various plant crops have revealed that, despite the complex microbial biodiversity of the soil, the bacterial microbiome is characterised by multiple functionalities. A better understanding of the molecular mechanisms, underlying the interactions between plants and the microbiome, could enable better development of plants, related to the beneficial action of microorganisms. Therefore, this review aims to describe the characteristics of the rhizosphere microbiome with the interactions that occur between soil and roots, as well as the …

Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates

Department of Animal Science: Faculty Publications

In August 2021, the U.S. Environmental Protection Agency (EPA) released draft models to estimate daily NH₃, H₂S, PM₁₀, PM_2.5, and TSP emissions from egg-layer houses (high-rise and manure-belt) and manure storage using inputs of daily mean ambient temperature, relative humidity (RH), and hen inventory. These models were developed from refined datasets generated by the National Air Emissions Monitoring Study fieldwork completed in 2009. Notably, they do not include data for cage-free housing. Currently, 66% of U.S. laying hens are housed in cages; thus, these models, if adopted, will have a substantial impact …

Novel Mechanisms Of Systemic Sclerosis-Associated Lung Fibrosis, Joe E. Mouawad

MUSC Theses and Dissertations

Systemic sclerosis (SSc), also known as scleroderma, is an autoimmune disorder that affects the connective tissues and has the highest mortality rate among the rheumatic diseases. One of the hallmarks of SSc is fibrosis, which may develop systemically, affecting the skin and virtually any visceral organ in the body. Fibrosis of the lungs leads to interstitial lung disease (ILD), which is currently the leading cause of death in SSc. The identification of effective treatments to stop or reverse lung fibrosis has been the main challenge in reducing SSc mortality and improving patient outcomes and quality of life. Currently, only two …

Canopy-Top Measurements Do Not Accurately Quantify Canopy-Scale Leaf Thermoregulation, Josef C. Garen, Luiza Maria T. Aparecido, Benjamin W. Blonder, Molly A. Cavaleri, Martijn Slot, Sean T. Michaletz

Michigan Tech Publications, Part 1

No abstract provided.

A Diverse Flea (Siphonaptera) Assemblage From The Small Mammals Of Central New Mexico, Dianne E. Peterson, Jonathan L. Dunnum, Schuyler W. Liphart, Karen A. Boegler, Ralph P. Eckerlin, Eric P. Hoberg, Martha O. Perez-Arriaga, Katrina Derieg, David Garnand, Mariel L. Campbell, Joseph A. Cook

MANTER: Journal of Parasite Biodiversity

The geographical ranges of many mammals and their associated parasites are dynamic. Comprehensive documentation of these communities over time provides a foundation for interpreting how changing environmental conditions, driven by accelerating climate change, other anthropogenic disturbances, and natural events, may influence host-parasite interactions. Fleas (Order Siphonaptera) are obligate, hematophagous parasites of birds and mammals with medical interest because of their role in transmitting pathogens. From 2016 to 2019, we sampled the small mammal and associated flea communities in El Malpais National Conservation Area (El Malpais) in Cibola County, New Mexico. Among 898 mammalian specimens, 925 fleas representing 29 species were …

Site-Specific Analysis Of The Incidence Rate Of Enterotoxigenic Escherichia Coli Infection Elucidates An Association With Childhood Stunting, Wasting, And Being Underweight: A Secondary Analysis Of The Mal-Ed Birth Cohort, Md Ahshanul Haque, Sabiha Nasrin, Parag Palit, Rina Das, Barbie Zaman Wahid, Md. Amran Gazi, Mustafa Mahfuz, Abu Syed Golam Faruque, Tahmeed Ahmed

Department of Nutrition and Health Sciences: Faculty Publications

Asymptomatic infection by fecal enteropathogens is a major contributor to childhood malnutrition. Here, we investigated the incidence rate of asymptomatic infection by enterotoxigenic Escherichia coli (ETEC) and assessed its association with childhood stunting, wasting, and being underweight among children under 2 years of age. The Malnutrition and Enteric Disease birth cohort study included 1,715 children who were followed from birth to 24 months of age from eight distinct geographic locations including Bangladesh, Brazil, India, Peru, Tanzania, Pakistan, Nepal, and South Africa. The TaqMan array card assay was used to determine the presence of ETEC in the nondiarrheal stool samples collected …

Impacts Of Floodplain Restoration On Water Temperature And Macroinvertebrates In Whychus Creek, Oregon, Wesley Nathan Noone

Dissertations and Theses

Stream restoration is a proposed climate adaptation tool, however, outcomes of floodplain restoration on stream temperature have been debated. Despite a growing number of studies that investigated water temperature in restored streams, few have quantified thermal heterogeneity in new habitat types created by restored hydrogeomorphic processes and the impact of thermal diversity on the aquatic macroinvertebrate community. In this study, I evaluated three hypotheses: 1) restoration increases habitat diversity, 2) habitat diversity increases water temperature heterogeneity, and 3) restored reaches have more diverse macroinvertebrate communities. I collected a total of 40 macroinvertebrate samples and characterized environmental conditions in three reaches …

Svip Dependent Tubular Lysosomal Restoration: A New Direction In Vcp Diseases, Ankita Basu

LSU Doctoral Dissertations

Multisystem Proteinopathy-1 (MSP-1) is a rare, autosomal dominant, degenerative disease resulting from single amino acid mis-sense mutations in the AAA+ ATPase, Valosin containing protein (VCP). Patients with VCP mutations vary widely in their pathology and clinical penetrance, making it difficult to devise effective treatment strategies. To investigate the pathology of VCP- related disease, we engineered CRISPR knock-in Drosophila models for nine MSP-1 associated VCP mutations. These mutant animals mimic human MSP-1 disease phenotypes, including progressive mobility decline and accumulation of protein aggregates, making them excellent models to study the pathology of VCP associated mutations and test potential treatment strategies. At …

Increases In Group A Streptococcal Infections In The Pediatric Population In Houston, Tx, 2022, Aya Aboulhosn, Misu A Sanson, Luis Alberto Vega, Maria G Segura, Lauren M Summer, J Chase Mcneil

Faculty, Staff and Student Publications

Beginning in October 2022, we observed a substantial increase in the total number of cases of invasive GAS disease (iGAS) in the pediatric population in Houston, TX. Emm12 GAS strains were disproportionately represented but the overall proportion of iGAS infections observed during the current spike was similar to pre-pandemic years.

Il6 Mediates Suppression Of T- And Nk-Cell Function In Emt-Associated Tki-Resistant Egfr-Mutant Nsclc, Sonia A Patel, Monique B Nilsson, Yan Yang, Xiuning Le, Hai T Tran, Xiaoxing Yu, Alissa Poteete, Xiaoyang Ren, Li Shen, Jing Wang, Seyed Javad Moghaddam, Tina Cascone, Michael Curran, Don L Gibbons, John V Heymach

Faculty, Staff and Student Publications

Purpose: Patients with advanced non-small cell lung cancer (NSCLC) harboring activating EGFR mutations are initially responsive to tyrosine kinase inhibitors (TKI). However, therapeutic resistance eventually emerges, often via secondary EGFR mutations or EGFR-independent mechanisms such as epithelial-to-mesenchymal transition. Treatment options after EGFR-TKI resistance are limited as anti-PD-1/PD-L1 inhibitors typically display minimal benefit. Given that IL6 is associated with worse outcomes in patients with NSCLC, we investigate whether IL6 in part contributes to this immunosuppressed phenotype.

Experimental design: We utilized a syngeneic genetically engineered mouse model (GEMM) of EGFR-mutant NSCLC to investigate the effects of IL6 on the tumor microenvironment and …

Dysregulation And Epigenetic Reprogramming Of Nrf2 Signaling Axis Promote Acquisition Of Cisplatin Resistance And Metastasis In Head And Neck Squamous Cell Carcinoma, Abdullah A Osman, Emre Arslan, Mason Bartels, Chieko Michikawa, Antje Lindemann, Wangjie Yu, Wencai Ma, Li Shen, Jing Wang, Anand K Singh, Mitchell J Frederick, Nakia D Spencer, Jeffery Kovacs, Timothy Heffernan, William F Symmans, Kunal Rai, Jeffrey N Myers

Faculty, Staff and Student Publications

PURPOSE: Cisplatin (CDDP)-based chemotherapy is a first-line treatment for patients with advanced head and neck squamous cell carcinomas (HNSCC), despite a high rate of treatment failures, acquired resistance, and subsequent aggressive behavior. The purpose of this study was to study the mechanism of CDDP resistance and metastasis in HNSCC. We investigated the role of NRF2 pathway activation as a driven event for tumor progression and metastasis of HNSCC.

EXPERIMENTAL DESIGN: Human HNSCC cell lines that are highly resistant to CDDP were generated. Clonogenic survival assays and a mouse model of oral cancer were used to examine the impact of NRF2 …

Longitudinal Global Transcriptomic Profiling Of Preclinical Systemic Sclerosis Reveals Molecular Changes Associated With Disease Progression, Chiara Bellocchi, Lorenzo Beretta, Xuan Wang, Marka A Lyons, Maurizio Marchini, Vincenzo Carbonelli, Shervin Assassi

Faculty, Staff and Student Publications

Objective: To investigate peripheral blood cell (PBCs) global gene expression profile of SSc at its preclinical stage (PreSSc) and to characterize the molecular changes associated with progression to a definite disease over time.

Material and methods: Clinical data and PBCs of 33 participants with PreSSc and 16 healthy controls (HCs) were collected at baseline and follow-up (mean 4.2 years). Global gene expression profiling was conducted by RNA sequencing and a modular analysis was performed.

Results: Comparison of baseline PreSSc to HCs revealed 2889 differentially expressed genes. Interferon signalling was the only activated pathway among top over-represented pathways. Moreover, 10 modules …

Inhibition Of Stat6 With Antisense Oligonucleotides Enhances The Systemic Antitumor Effects Of Radiotherapy And Anti-Pd-1 In Metastatic Non-Small Cell Lung Cancer, Kewen He, Hampartsoum B Barsoumian, Nahum Puebla-Osorio, Yun Hu, Duygu Sezen, Genevieve Bertolet, Carola Leuschner, Liangpeng Yang, Claudia S Kettlun Leyton, Natalie Wall Fowlkes, Morgan Maureen Green, Lisa Hettrick, Dawei Chen, Fatemeh Masrorpour, Meidi Gu, Hadi Maazi, Alexey S Revenko, Maria Angelica Cortez, James W Welsh

Faculty, Staff and Student Publications

Diverse factors contribute to the limited clinical response to radiotherapy (RT) and immunotherapy in metastatic non-small cell lung cancer (NSCLC), among which is the ability of these tumors to recruit a retinue of suppressive immune cells-such as M2 tumor-associated macrophages (TAM)-thereby establishing an immunosuppressive tumor microenvironment that contributes to tumor progression and radio resistance. M2 TAMs are activated by the STAT6 signaling pathway. Therefore, we targeted STAT6 using an antisense oligonucleotide (ASO) along with hypofractionated RT (hRT; 3 fractions of 12 Gy each) to primary tumors in three bilateral murine NSCLC models (Lewis lung carcinoma, 344SQ-parental, and anti-PD-1-resistant 344SQ lung …

Intratumoral Heterogeneity And Clonal Evolution Induced By Hpv Integration, Keiko Akagi, David E Symer, Medhat Mahmoud, Bo Jiang, Sara Goodwin, Zhengke Li, Joe Dan Dunn, Thomas Ried, Kevin R Coombes, Fritz J Sedlazeck, Maura L Gillison

Faculty, Staff and Student Publications

The human papillomavirus (HPV) genome is integrated into host DNA in most HPV-positive cancers, but the consequences for chromosomal integrity are unknown. Continuous long-read sequencing of oropharyngeal cancers and cancer cell lines identified a previously undescribed form of structural variation, "heterocateny," characterized by diverse, interrelated, and repetitive patterns of concatemerized virus and host DNA segments within a cancer. Unique breakpoints shared across structural variants facilitated stepwise reconstruction of their evolution from a common molecular ancestor. This analysis revealed that virus and virus-host concatemers are unstable and, upon insertion into and excision from chromosomes, facilitate capture, amplification, and recombination of host …