Life Sciences Commons^™

Plant Sentience And The Case For Ethical Veganism, Josh Milburn

Animal Sentience

Does the possibility of plant sentience pose a problem for ethical veganism? It has not yet been demonstrated that plants are sentient (i.e., that they can feel). Moreover, even if it were demonstrated that plants could feel, it would also have to be demonstrated that they can feel the affectively “valenced” feelings that are ethically significant, such as pain and fear, rather than just neutral sensations such as darker/lighter, or wetter/drier. Finally, if plants could feel valenced feelings, veganism would likely still be the ethical option, on the principle of causing the least harm.

Physiological And Transcriptomic Responses Of Two Artemisia Californica Populations To Drought: Implications For Restoring Drought-Resilient Native Communities, Hagop S. Atamian Dr., Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

As climate change brings drier and more variable rainfall patterns to many arid and semi-arid regions, land managers must re-assemble appropriate plant communities for these conditions. Transcriptome sequencing can elucidate the molecular mechanisms underlying plant responses to changing environmental conditions, potentially enhancing our ability to screen suitable genotypes and species for restoration. We examined physiological and morphological traits and transcriptome sequences of coastal and inland populations of California sagebrush (Artemisia californica), a critical shrub used to restore coastal sage scrub vegetation communities, grown under low and high rainfall environments. The populations are located approximately 36 km apart but …

Prmt3-Mediated Arginine Methylation Of Igf2bp1 Promotes Oxaliplatin Resistance In Liver Cancer, Yunxing Shi, Yi Niu, Yichuan Yuan, Kai Li, Chengrui Zhong, Keren Li, Zhiwen Yang, Dinglan Zuo, Jiliang Qiu, Wei He, Chenwei Wang, Yadi Liao, Guocan Wang, Yunfei Yuan, Binkui Li

Faculty, Staff and Student Publications

Although oxaliplatin-based chemotherapy has been effective in the treatment of hepatocellular carcinoma (HCC), primary or acquired resistance to oxaliplatin remains a major challenge in the clinic. Through functional screening using CRISPR/Cas9 activation library, transcriptomic profiling of clinical samples, and functional validation in vitro and in vivo, we identify PRMT3 as a key driver of oxaliplatin resistance. Mechanistically, PRMT3-mediated oxaliplatin-resistance is in part dependent on the methylation of IGF2BP1 at R452, which is critical for the function of IGF2BP1 in stabilizing the mRNA of HEG1, an effector of PRMT3-IGF2BP1 axis. Also, PRMT3 overexpression may serve as a biomarker for oxaliplatin resistance …

Mutation-Agnostic Detection Of Colorectal Cancer Using Liquid Biopsy-Based Methylation-Specific Signatures, Mohamed A Gouda, Dzifa Y Duose, Morten Lapin, Stephanie Zalles, Helen J Huang, Xiaofeng Zheng, Alshimaa M Alhanafy, Mohamed A Shehata, Jing Wang, Scott Kopetz, Funda Meric-Bernstam, Ignacio I Wistuba, Rajyalakshmi Luthra, Filip Janku

Faculty, Staff and Student Publications

Detection of methylation patterns in circulating tumor DNA (ctDNA) can offer a novel approach for cancer diagnostics given the unique signature for each tumor type. We developed a next-generation sequencing (NGS)-based assay targeting 32 CpG sites to detect colorectal cancer-specific ctDNA. NGS was performed on bisulfite-converted libraries and status dichotomization was done using median methylation ratios at all targets. We included plasma samples from patients with metastatic colorectal (n = 20) and non-colorectal cancers (n = 8); and healthy volunteers (n = 4). Median methylation ratio was higher in colorectal cancer compared with non-colorectal cancers (P = .001) and normal …

An Expedited Strategy For Accurate And Timely Integrated Molecular Diagnosis Of Gliomas, Timothy A Gregory, Garret L Williford, Jacob M Maronge, Kristin Alfaro, Gregory N Fuller, Vinay K Puduvalli, Nazanin K Majd

Faculty, Staff and Student Publications

No abstract provided.

A Distinct Pattern Of Growth And Rac1 Signaling In Melanoma Brain Metastasis Cells, Ioana Stejerean-Todoran, Phyllis A Gimotty, Andrea Watters, Patricia Brafford, Clemens Krepler, Haiyin Li, Anke Zieseniss, Dörthe M Katschinski, Sinem M Sertel, Silvio O Rizzoli, Bradley Garman, Katherine L Nathanson, Xiaowei Xu, Qing Chen, Jack H Oswald, Michal Lotem, Gordon B Mills, Michael A Davies, Michael P Schön, Ivan Bogeski, Meenhard Herlyn, Adina Vultur

Faculty, Staff and Student Publications

Background: Melanoma, the deadliest of skin cancers, has a high propensity to form brain metastases that are associated with a markedly worsened prognosis. In spite of recent therapeutic advances, melanoma brain lesions remain a clinical challenge, biomarkers predicting brain dissemination are not clear and differences with other metastatic sites are poorly understood.

Methods: We examined a genetically diverse panel of human-derived melanoma brain metastasis (MBM) and extracranial cell lines using targeted sequencing, a Reverse Phase Protein Array, protein expression analyses, and functional studies in vitro and in vivo.

Results: Brain-specific genetic alterations were not detected; however, MBM cells in vitro …

Brain Metastases From Biliary Tract Cancer: Case Series And Clinicogenomic Analysis, Grace N Dodoo, Brian De, Sunyoung S Lee, Joseph Abi Jaoude, Jean-Nicolas Vauthey, Hop S Tran Cao, Jacob J Mandel, Thomas H Beckham, Bruce D Minsky, Grace L Smith, Emma B Holliday, Albert C Koong, Prajnan Das, Cullen M Taniguchi, Milind Javle, Eugene J Koay, Ethan B Ludmir

Faculty, Staff and Student Publications

BACKGROUND: Limited data from small series have suggested that brain metastases from biliary tract cancers (BrM-BTC) affect ≤2% of patients with BTC. We sought to review our experience with patients with BrM-BTC and to identify associations of tumor-related molecular alterations with outcomes.

MATERIALS AND METHODS: A retrospective review of patients with BTC seen at a tertiary referral center from 2005 to 2021 was performed; patients with BrM-BTC were identified, and clinical and molecular data were collected.

RESULTS: Twenty-one of 823 patients with BTC (2.6%) developed BrM. For patients with BrM-BTC, median follow-up time was 27.9 months after primary BTC diagnosis …

Evaluation Of Concussion Assessment Tools For Collegiate Athletes, Jacqueline D. Villanueva Arevalo, Emily R. Brown

Student Scholar Showcase

BACKGROUND: Due to the increase in sports-related concussions in the United States, there is a rising concern about concussion assessments in collegiate athletes with regard to long term vestibular ocular motor dysfunction and post-concussion syndrome.

PURPOSE: Due to varying practices in concussion diagnosis, this systematic review analyzed three concussion diagnostic instruments, The King Devick, Screening Concussion Assessment Tool (SCAT-5), and Vestibular Oculomotor Screening Tool (VOMS), to determine the most appropriate instrument(s) for reducing vestibular ocular-motor dysfunction and post-concussion symptoms in collegiate athletes.

METHODS: Nested Knowledge was used to identify articles from PubMed, Europe Pubmed Central, and ClinicalTrials.gov databases. Key search …

Effect Of Substitution Time On Physical, Technical And Cognitive Performance In Sub-Elite Male Field Hockey Players, Thomas W. Spooner, Andrew T. West, Mark Et Willems

International Journal of Exercise Science

International Journal of Exercise Science 16(6): 497-512, 2023. We examined the effects of substitution time (i.e., recovery time) in a simulated field hockey test on physical, technical and perceptual/cognitive performance. Nine sub-elite male field hockey players (age: 20 ± 2 yrs, height: 1.81 ± 0.06 m, body mass: 71 ± 10 kg, body fat: 10.3 ± 3.7%, O_2max: 67 ± 3 completed four 8-min 40-s bouts of high-intensity intermittent exercise with 2-min and 5.5-min substitution time replicating the demands of a 4-quarter field hockey match. After each bout, a 15-m maximal sprint, agility/dribbling test, passing accuracy test, and …

Effect Of Neuromuscular Electrical Stimulation Training On Control Of Involuntary Muscular Torque And Stimulation Intensity In Older Adults, Kyndall P. Ramirez, Nigel C. Jiwan, Joni A. Mettler Ph.D

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 482-496, 2023. The purpose of this study was to examine the effects of a 4-week neuromuscular electrical stimulation (NMES) training regimen on involuntary torque output and electrical stimulation intensity in older adults. Twelve older adults (ages: 68.4 ± 6.5 years; men: n = 6, women: n = 6; weight: 158.6 ± 27.3 lbs; height: 65.2 ± 2.1 in) received submaximal intensity NMES to the quadriceps for 4 weeks to determine training-related changes in stimulation intensity and involuntary control of muscular torque during the NMES protocol. Two-way repeated measures ANOVAs were used to compare torque …

Locomotor Biomechanics In Stream Gobies, Rhinogobius Sp.: Functional Performance Of Waterfall-Climbing Behavior, Ernest W. Murphy

Student Scholar Showcase

Gobiidae is the taxonomic classification of a large fish family comprising over 2,000 species. A few goby species have the unique ability of scaling waterfalls using pelvic fins that are fused into a suction disc (pelvic sucker) located on the ventral side of the body, aiding them in upstream migration to freshwater feeding and reproduction locations. This study aimed to investigate the locomotor biomechanics of Rhinogobius goby fish due to the lack of literature in the area. In particular, climbing performance variables (pressure differential, force, endurance, and kinematics of attachment), pelvic sucker fatigability, and ontogenetic patterns of physical development and …

Analysis Of Attitudinal Student Learning Benefits From A Course-Based Undergraduate Research Experience (Cure) Adapted For Online Format, Ambika Kapil, Luis C. Gonzalez Isoba, Niraj Pathak, Arthur Sikora, Santanu De

FDLA Journal

Course-based Undergraduate Research Experiences (CUREs) are an increasingly utilized model for exposing students to research. The lack of robust assessments is a major hurdle to wider adoption of CUREs. The Coronavirus Infectious Disease 2019 (COVID-19) pandemic necessitated a drastic shift of in-person courses to the online format. Using the Participant Perception Indicator (PPI) survey, we measured students’ self-reported changes in learning from such a biochemistry course at a large university in south Florida based on the Biochemistry Authentic Scientific Inquiry Lab (BASIL) model. By doing this, we were able to better understand the student-benefits of CUREs and how these benefits …

Spatiotemporally Dynamic Electric Fields For Brain Cancer Treatment: An In Vitro Investigation, Erin Iredale, Abdulla Elsaleh, Hu Xu, Paul Christiaans, Andrew Deweyert, John Ronald, Susanne Schmid, Matthew O Hebb, Terry M Peters, Eugene Wong

Anatomy and Cell Biology Publications

Objective. The treatment of glioblastoma (GBM) using low intensity electric fields (∼1 V cm^-1) is being investigated using multiple implanted bioelectrodes, which was termed intratumoral modulation therapy (IMT). Previous IMT studies theoretically optimized treatment parameters to maximize coverage with rotating fields, which required experimental investigation. In this study, we employed computer simulations to generate spatiotemporally dynamic electric fields, designed and purpose-built an IMT device for in vitro experiments, and evaluated the human GBM cellular responses to these fields. Approach. After measuring the electrical conductivity of the in vitro culturing medium, we designed experiments to evaluate the …

Application Of A Novel Lytic Jerseyvirus Phage Lpsent1 For The Biological Control Of The Multidrug-Resistant Salmonella Enteritidis In Foods, Rashad R. Al-Hindi, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Khloud M. Algothmi, Ahmed Esmael

Nebraska Center for Virology: Faculty Publications

Non-typhoidal Salmonella is the tremendously predominant source of acquired foodborne infection in humans, causing salmonellosis which is a global threat to the healthcare system. This threat is even worse when it is combined with the incidence of multidrug-resistant Salmonella strains. Bacteriophage therapy has been proposed as a promising potential candidate to control a diversity of foodborne infective bacteria. The objective of this study designed to isolate and characterize lytic phages infecting zoonotic multi-drug resistant and strong biofilm producer Salmonella enterica serovar Enteritidis EG.SmE1 and then apply the isolated phage/s as a biocontrol agent against infections in ready-to-eat food articles including …

The Application Of Microbial Source Tracking To Aid In Site Prioritization For Remediation In Lower Michigan, John J. Hart

Masters Theses

Non-point source fecal pollution is a threat to both the environment and public health. Climate change, aging infrastructure, and intensified agricultural practices are predicted to accentuate this issue. In Michigan, due to the high instance of aging infrastructure and intensified agriculture, non-point source fecal pollution has caused many waterbodies to exceed the state standards posing a risk to recreational activities and source water. Due to this threat, there is an increased effort to identify and remediate these sources. My study focused on improving the identification of non-point source fecal pollution through a combination of culture-based and molecular fecal indicator bacteria …

Evaluation Of Reproductive Phenology, Space Use, And Ecology Of The Eastern Wild Turkey (Meleagris Gallopavo Silvestris) In West-Central Louisiana, Chad Argabright

LSU Master's Theses

Nest site selection is a driving demographic force behind eastern wild turkey (Meleagris gallopavo silvestris) populations. However, previous research was likely not focused on the actual time of nest site selection, considering that nest site selection is likely only able to occur on the day of the first egg being laid. My objective was to determine if selection for any vegetation characteristics was occurring on the first day of laying. I estimated the path taken from the roost to the nest on the first day of egg laying (i.e., laying path) using GPS data collected from 164 unique …

The Role Of The Pcc And Acc In Autism Spectrum Disorder: A Functional Connectivity Study, Claudia Gonzalez

Honors Theses

Background: Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder that impacts behavior control and social skills. Currently, ASD diagnoses are largely qualitative; thus, having more quantitative means of identification would aid in earlier diagnosis of ASD. However, more research is needed to pinpoint observable neurobiological markers of ASD. Our study focused on the posterior cingulate cortex (PCC) due to past evidence suggesting its involvement in ASD symptomatology. The PCC has been linked to behavioral control and executive functioning. Moreover, previous studies support executive functioning’s responsibility for the repetitive and restricted behaviors (RRB) in ASD.

Method: Our study sought to …

The Relationship Between Blood Lactate And Heart Rate Responses To An Established Biking Protocol In Female Distance Runners And Sedentary Females, Haleigh Kozlowski

Undergraduate Theses and Capstone Projects

The aim of this study is to examine the differences in heart rate and lactic thresholdbetween female distance athletes and sedentary females. Lactate threshold is one of the variables used to measure anaerobic threshold. Anaerobic threshold characterizes the point when metabolic acidosis, along with the associated changes in gas exchange in the lungs, occurs during exercise. In this experiment, 16 female subjects -- 8 sedentary (174.56cm + 5.06 cm, 72.79 kg + 11.61kg), and 8 distance athletes (169.24cm + 8.58 cm, 57.44 kg + 5.37kg) -- were asked to ride a stationary bike for 18 minutes at 60 rotations per …

Characterizing Marr Homologs That Mediate Bacterial Fitness And Survival, George Chukwuemeka Nwokocha

LSU Doctoral Dissertations

Plant disease outbreaks are on the rise, threatening food security in many parts of the globe. To address these enormous challenges, new intervention strategies like bio-controls are being used as an important sustainable alternative to mitigate the negative effects of pathogens and increase the quality and productivity of agricultural crops. MarR family transcriptional regulators have been studied for their involvement in regulating bacterial virulence genes that help colonize plant hosts and therefore could serve as targets for biocontrol. The transcriptional regulator PecS, a member of the MarR family, is encoded by select bacterial pathogens and we show here that PecS …

Development, Optimization, And In Vitro/In Vivo Evaluation Of Azelaic Acid Transethosomal Gel For Antidermatophyte Activity, Ali Nasr, Noha Badawi, Yasmine Tartor, Nader Sobhy, Ali Nasr

Pharmacy

Treatment of dermatophytosis is quite challenging. This work aims to investigate the antidermatophyte action of Azelaic acid (AzA) and evaluate its efficacy upon entrapment into transethosomes (TEs) and incorporation into a gel to enhance its application. Optimization of formulation variables of TEs was carried out after preparation using the thin film hydration technique. The antidermatophyte activity of AzA-TEs was first evaluated in vitro. In addition, two guinea pig infection models with Trichophyton (T.) mentagrophytes and Microsporum (M.) canis were established for the in vivo assessment. The optimized formula showed a mean particle size of 219.8 ± 4.7 nm and a …

The Sars-Cov-2 Utr’S Intrudes Host Rbp’S And Modulates Cellular Splicing, Anjali Singh, Kush Kumar Pandey, Shubham Kumar Agrawal, Rupesh K. Srivastava, Sankar Bhattacharyya, Bhupendra Verma

Nebraska Center for Virology: Faculty Publications

SARS-CoV-2 is a novel coronavirus that causes a potentially fatal respiratory disease known as coronavirus disease (COVID-19) and is responsible for the ongoing pandemic with increasing mortality. Understanding the host-virus interaction involved in SARS-CoV-2 pathophysiology will enhance our understanding of the mechanistic basis of COVID-19 infection. The characterization of post-transcriptional gene regulatory networks, particularly pre-mRNA splicing, and the identification and characterization of host proteins interacting with the 5' and 3'UTRs of SARS-CoV-2 will improve our understanding of posttranscriptional gene regulation during SARS-CoV-2 pathogenesis. Here, we demonstrate that either SARS-CoV-2 infection or exogenous overexpression of the 5' and 3’UTRs of the …

Late Postemergence Glufosinate-Based Programs For Glyphosate-Resistant Palmer Amaranth Control In Dicamba/Glufosinate/Glyphosate-Resistant Soybean, Vipan Kumar, Jatinder S. Aulakh, Rui Liu, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant (GR) Palmer amaranth (Amaranthus palmeri S. Watson) is widespread in the Central Great Plains. Introduction of newly developed dicamba/glufosinate/glyphosate (DGG)-resistant soybean varieties allows postemergence (POST) applications of dicamba and glufosinate for in-season control of GR Palmer amaranth. Limited information exists on the effectiveness of glufosinate applied late-POST for tall (70–90 cm) GR Palmer amaranth control in DGG-resistant soybean. The objectives of this study were to (1) determine the effectiveness of late-POST glufosinate-based programs for GR Palmer amaranth control, and (2) determine the impact of those programs on soybeans grain yields. Ten glufosinatebased programs were tested in a field …

Evaluating The Pesticide-Induced Outbreak Of Soybean Looper, Chrysodeixis Includens (Walker) (Lepidoptera: Noctuidae), In Louisiana Soybean, Scott Lee

LSU Doctoral Dissertations

Soybean looper, Chrysodeixis includens (Walker), has established itself as the primary lepidopteran defoliator in Midsouth soybean agroecosystems. Pesticide-induced outbreak events associated with this pest have been observed by soybean producers. The insecticides believed to prompt these incidents are routinely used for the control of other co-occuring pests, such as stink bugs. Outbreak conditions present soybean producers with conflicting management decisions for pests. Laboratory, greenhouse, and field experiments were conducted to provide a better understanding of insecticide usage impact on populations of C. includens and the arthropod community. To discern the potential mechanisms behind C. includens outbreak, a baseline for the …

The Global Transcription Factor Fur Is Activated By Binding A [2fe-2s] Cluster To Maintain Iron Homeostasis In Escherichia Coli, Chelsey Rene Fontenot

LSU Doctoral Dissertations

Iron is an essential element for biological functions. Organisms have developed specific mechanisms to acquire, store, and export iron to manage intracellular iron homeostasis. These mechanisms are crucial not only for providing the cells with adequate iron for biological processes but also for ensuring that cells are not overloaded with excess iron, which is highly toxic. The Ferric uptake regulator (Fur) is a global transcription factor for maintaining intracellular iron homeostasis in bacteria. Previously, it was postulated that when the intracellular iron content is elevated, Fur binds ferrous iron to down-regulate the iron acquisition genes and up- regulate the iron …

The Regulation Of Autoimmunity Genes In Pancreatic Β-Cells By Nf-Κb, Thomas Malone Martin

LSU Doctoral Dissertations

Type 1 diabetes (T1D) is an autoimmune disease where the immune system targets the pancreatic islet beta-cells, which are responsible for insulin production and secretion, leading to a state of insulin insufficiency. Many of the molecular details of disease onset and progression remain poorly understood, including key aspects of cytokine signaling. Therefore, we have examined some of the consequences of inflammatory cytokine signaling as a critical module relevant to T1D. Using interleukin-1 (IL-1) signaling as a well-defined paradigm for inflammation, we investigated key components of this signaling pathway in pancreatic beta-cells. We identified ICAM-1, one of the proteins expressed in …

Inorganic Carbon Outwelling From A Mediterranean Seagrass Meadow Using Radium Isotopes, Claudia Majtényi-Hill, Gloria Reithmaier, Yvonne Y. Y. Yau, Oscar Serrano, Nerea Piñeiro-Juncal, Isaac R. Santos

Research outputs 2022 to 2026

Seagrass meadows are ‘blue carbon’ ecosystems widely recognised for their potential role in climate change mitigation. Previous studies have focused mainly on carbon storage within meadows and sediments. However, little is known about contribution of outwelling (i.e., lateral transport) to seagrass carbon budgets. Here, radium isotopes (²²³Ra and ²²⁴Ra) were used to assess dissolved inorganic carbon (DIC) and total alkalinity (TA) outwelling from a Mediterranean Posidonia oceanica meadow during early autumn. DIC outwelling was 114 ± 61 mmol m ^{− 2} day ^{− 1} and exceeded above-meadow CO2 outgassing (3 ± 1 mmol m ^{− 2} day …

Map The System: Presence And Impact Of Ppcps In Water, Emma Vandermeulen, Ruby Mitchell

OHSU-PSU School of Public Health Annual Conference

In a changing climate, there is growing emphasis on protecting our water sources and developing systems for conserving and reusing water. While many contaminants of concern are monitored and addressed by water treatment systems, pharmaceuticals and personal care products (PPCPs) are generally not accounted for. PPCPs in water systems have been an increasing concern as detection of these products has increased over the last few decades. Studies have been exploring the impact of antibiotics, antidepressants, contraceptives, and other anthropogenic products on our water system and how these substances interact with local ecosystems. Our main research questions are to understand what …

Adaptive Plasticity Of Coloration In Response To Environmental Change, Karissa Coffield

Scholars Week

When rapid environmental changes occur, different selective forces can create phenotypic trade-offs in which a trait can provide fitness benefits or costs under different environmental conditions. Amphibians are particularly vulnerable to environmental change, and previous research has revealed that some species will plastically respond to variation in temperature and ultra-violet radiation (UVR) by altering their coloration. Divergent selection on coloration may change with elevation and climate induced shifts in temperature because high temperatures are likely to result in lighter color morphs but as elevation increases, UVR exposure increases leading to the prediction that darker color morphs will be more common. …

Evaluación Y Rentabilidad De Variedades De Chile En Tres Granjas De Minnesota Y Wisconsin, 2022, Natalie Hoidal, Charlie Rohwer, Ryan Pesch, Rodrigo Cala, Javier García, Griselda Bernabe Suarez, Fernando Alejandro García Loyo

Midwest Vegetable Trial Reports

Los agricultores latinos de hortalizas frescas de Minnesota y Wisconsin están interesados en cultivar chiles picantes tanto frescos como deshidratados para su venta en mercados locales. Este ensayo de variedades es el tercero y último de un proyecto de tres años de duración. El ensayo incluyo 14 variedades de chile incluyendo jalapeño, serrano, habanero, poblano y "otros" tipos. Las variedades incluyeron las mejores variedades de 2020 y 2021 junto con nuevas variedades. Calculamos el rendimiento, tamaño y las unidades Scoville. Además del ensayo de variedades, realizamos un análisis empresarial para determinar la rentabilidad de la producción de chiles picantes en …

Evaluating Form And Function Of Groundcovers And Their Environmental Impacts In Louisiana Landscapes, Thomas Maxwell Mckeown

LSU Master's Theses

Increasing environmental concerns are encouraging the adoption of sustainable landscapes that have environmental, social, and financial benefits. Ornamental groundcover systems are touted as sustainable landscape features due to the associated decreased demand of water, fertilizer, pesticide, and maintenance; however, limited research exists on soil property effects, planting density, weed density, or consumer preferences of groundcovers. This study was aimed to gain a more thorough understanding of ornamental groundcovers systems and their environmental impacts. The effects of groundcover growth habit (matting; bunching) and irrigation delivery (micro spray; overhead) on soil temperature, volumetric water content (VWC), and electric conductivity (EC) along with …