Life Sciences Commons^™

Developmental Exposure To Corn Grown On Lake Erie Dredged Material: A Preliminary Analysis, Kaylyn A. S. Flanigan, Madelyn I. Czuba, Victoria R. Riesgo, Megan A. Rúa, Louise M. Stevenson, Jari Willing

Biological Sciences Faculty Publications

While corn is considered to be a healthy food option, common agricultural practices, such as the application of soil amendments, might be introducing contaminants of concern (COC) into corn plants. The use of dredged material, which contain contaminants such as heavy metals, polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs), as a soil amendment is increasing. Contaminants from these amendments can accumulate in corn kernels harvested from plants grown on these sediments and potentially biomagnify in organisms that consume them. The extent to which secondary exposure to such contaminants in corn affect the mammalian central nervous system has been virtually …

Promoting Engagement Through Socioscientific Inquiry At The Middle School Level, Kaitlyn Kalehuawehe

Undergraduate Theses

Learning science is not just about facts. It is an area that has the potential to expand beyond the classroom. There is a concern for students having negative associations with science, and not seeing the relevance it plays within their lives. The curriculum should reflect science's value within society and its influence on complex issues. Learning science in the context of socioscientific issues (SSI) can promote an understanding that connects science to society and everyday life. The main objective of this thesis is the see how the use of SSI in the middle school classroom can promote student engagement and …

The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran

Undergraduate Theses

Animal pollinators are the cornerstone of healthy ecosystems. Their survival is essential for the persistence of entire food chains: from the flowers they cross-pollinate directly, to the animals who depend on those plants for nutrition. The establishment of pollinator gardens—particularly ones that consist of native plants—is an effective way to enhance their biodiversity, abundance, and well-being.

The main goal of this thesis is to construct a pollinator garden that maximizes the benefits for animal pollinators using feedback from local gardeners. A survey was used to gather information about the popularity and preferences of 40 flowering plants, and after analyzing the …

A Potential Role Of Gsk-3beta In The Development Of Pain Post-Spinal Cord Injury, Emily Ernst

Undergraduate Theses

Chronic neuropathic pain is a very common consequence following spinal cord injury (SCI) and poses significant clinical challenge. Current treatments are largely ineffective and as a result, patients are often left with debilitating pain at and below the level of the spinal cord injury. After spinal injury, a cascade of events occurs within the nervous system. One important aspect of this cascade for investigation is the anatomical changes that occur post-SCI. There is evidence of maladaptive sprouting/growth in sensory nerve fibers that is thought to play a role in the development and amplification of pain signals. Sprouting of primary afferents …

Migration Of Elk (Cervus Canadensis) And Barriers To Movement, Levi Watkins

Theses and Dissertations

Movement patterns of animals are varied, complex, and can be influenced by environmental and anthropogenic factors. One form of animal movement, migration, is influenced by environmental factors that alter the timing, duration, intensity, and likelihood of migration. Additionally, features of the landscape, both natural and anthropogenic, can alter how animals move through their seasonal and home ranges. Movement patterns can be impeded or prevented by features such as railroads, rivers, and roads. Here we explore characteristics of elk (Cervus canadensis) migration by evaluating the strategies of migration exhibited and the factors that influence migration of elk in central and eastern …

Overcoming Barriers To Native Species Restoration Using Gibberellic Acid And Fungicide Seed Coatings, Amber Jo Johnson

Theses and Dissertations

Many barriers can limit restoration success. In the first chapter of this thesis, the barrier of strong seed dormancy is addressed. While dormancy benefits the species' long-term survival, it can present a challenge within a restoration scenario where rapid establishment is required. Soaking seeds in gibberellic acid (GA3) can overcome dormancy. An easier and potentially more effective method to apply this hormone is to coat seeds with a GA3-impregnated polymer, which provides a slow release of the hormone. Seed dormancy can also be mitigated by creating a favorable microsite with increased soil moisture. We compared the emergence and establishment of …

Fuel Your Fitness, Payton Carpenter

Senior Seminar Papers

Caffeinated beverages are incredibly popular for improving mental acuity and exercise performance, and their ergogenic effects have been thoroughly studied. For our research project, we wanted to study the effects of caffeine, specifically caffeinated energy drinks, on muscular endurance. There are hundreds of energy drinks on the market with varying ingredients in addition to caffeine that claim to have substantial physiological benefits, so when choosing energy drinks for the study, we chose Alani Nu and Celsius because of the similarities in marketing strategies, ingredients, and serving size. We asked this questions: is there a significant difference in muscular endurance when …

Volatile Organic Compound Identification Using Metal Oxide Sensors And Machine Learning, Hunter Tiner

Theses and Dissertations

Volatile Organic Compounds (VOCs) are emitted gases that include many chemicals that can be created by biological processes or the byproduct of industrial processes, and can be present in a variety of pollutants. VOCs can cause a variety of ailments in humans and animals, and are expressed in plants as a way of signaling damage or the presence of pests. In this Thesis, an Internet of Things (IOT) E-nose device is outfitted with a gas array of eight Metal Oxide Semiconductor (MOS) type gas sensors, which are sensitive to a broad range of VOCs, yet lack selectivity and are unable …

Anti-Microbial Peptide Screen For Crop Protection, Sydney Chen

Honors Theses

Vestaron currently uses an established and reliable peptide discovery and development research platform, particularly for the development of insecticidal peptides. The platform centers around a proprietary yeast expression system for peptide production (“Vestaron’s yeast expression system"). Vestaron aims to expand their portfolio and provide clean solutions in crop protection using peptide technologies to protect plants not only from insect pests but also microbial pests including phytopathogenic fungi and bacteria. Antimicrobial peptides (AMPs) have been studied extensively. However, it is questionable whether Vestaron’s yeast expression system can express AMPs heterologously. In this study, thirteen promising antimicrobial peptides were selected from literature …

Long Term Monitoring Of Grand Ravines Park Restoration: An Authentic Research Opportunity For Jenison High School Students And Beyond, Karina C. White

Masters Projects

Access to authentic research is limited at the 7-12 science education level. At the same time, many local restoration projects would benefit from, but don’t have access to a long-term system of monitoring. This project seeks to unite those two needs by developing a protocol for 7-12 classrooms to be able to participate in authentic research through long-term monitoring of a local restoration project. The protocol developed in this project was used by Jenison High School students at Grand Ravines Park. Grand Ravines Park is a recently acquired Ottawa County park with a history of anthropogenic disturbances. Shortly after the …

Characterizing Movement Patterns Of Nomadic Pastoralists And Their Exposure To Rift Valley Fever In Kenya, G. Mosomtai, J. L. Kasiiti, R. M. Murithi, P. Sandström, T. Landmann, O. W. Lwande, O. A. Hassan, C. Ahlm, R. Sang, M. Evander, Z. Szantoi, G. Ottavianelli

All Peer-Reviewed Publications

The role of animal movement in spreading infectious diseases is highly recognized by various legislations and institutions such as the World Organisation for Animal Health and the International Animal Health Code. The increased interactions at the nexus of human-animal-ecosystem interface have seen an unprecedented introduction and reintroduction of new zoonotic diseases with high socio-economic impacts such as the COVID-19 pandemic. Rift Valley fever (RVF) is a zoonotic disease that affects both humans and animals and is transmitted by Aedes mosquitoes or through contact with the body fluids of infected animals. This study seeks to characterize movement patterns of pastoralist and …

Comparative Study Of Adenosine Analogs As Inhibitors Of Protein Arginine Methyltransferases And A Clostridioides Difficile- Specific Dna Adenine Methyltransferase, Jujun Zhou, Youchao Deng, Iredia D Iyamu, John R Horton, Dan Yu, Taraneh Hajian, Masoud Vedadi, Dante Rotili, Antonello Mai, Robert M Blumenthal, Xing Zhang, Rong Huang, Xiaodong Cheng

Faculty, Staff and Student Publications

S-Adenosyl-l-methionine (SAM) analogs are adaptable tools for studying and therapeutically inhibiting SAM-dependent methyltransferases (MTases). Some MTases play significant roles in host–pathogen interactions, one of which is Clostridioides difficile-specific DNA adenine MTase (CamA). CamA is needed for efficient sporulation and alters persistence in the colon. To discover potent and selective CamA inhibitors, we explored modifications of the solvent-exposed edge of the SAM adenosine moiety. Starting from the two parental compounds (6e and 7), we designed an adenosine analog (11a) carrying a 3-phenylpropyl moiety at the adenine N6-amino group, and a 3-(cyclohexylmethyl guanidine)-ethyl moiety at the …

Cross-Dataset Single-Cell Analysis Identifies Temporal Alterations In Cell Populations Of Primary Pancreatic Tumor And Liver Metastasis, Daowei Yang, Rohan Moniruzzaman, Hua Wang, Huamin Wang, Yang Chen

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) has a unique tumor microenvironment composed of various cell populations such as cancer cells, cancer-associated fibroblasts (CAFs), immune cells, and endothelial cells. Recently, single-cell RNA-sequencing analysis (scRNA-seq) has systemically revealed the genomic profiles of these cell populations in PDAC. However, the direct comparison of cell population composition and genomic profile between primary tumors (at both early- and late-stage) and metastatic tumors of PDAC is still lacking. In this study, we combined and analyzed recent scRNA-seq datasets of transgenic KPC mouse models with autochthonous PDAC and matched liver metastasis, revealing the unique tumor ecosystem and cell composition …

Wastewater Surveillance Of Sars-Cov-2 Genomic Populations On A Country-Wide Scale Through Targeted Sequencing, Florencia Cancela, Natalia Ramos, Davida S. Smyth, Et Al.

All Faculty Scholarship (Archived)

SARS-CoV-2 surveillance of viral populations in wastewater samples is recognized as a useful tool for monitoring epidemic waves and boosting health preparedness. Next generation sequencing of viral RNA isolated from wastewater is a convenient and cost-effective strategy to understand the molecular epidemiology of SARS-CoV-2 and provide insights on the population dynamics of viral variants at the community level. However, in low- and middle-income countries, isolated groups have performed wastewater monitoring and data has not been extensively shared in the scientific community. Here we report the results of monitoring the co-circulation and abundance of variants of concern (VOCs) of SARS-CoV-2 in …

Heavy Metal Content In Eggs From Wild And Managed-Care Humboldt Penguins, Mya Daniels-Abdulahad

All HCAS Student Capstones, Theses, and Dissertations

The Humboldt penguin, Spheniscus humboldti, has been recognized as a Vulnerable species (IUCN) for nearly two decades, with annual population declines of more than 10% largely attributed to pervasive anthropogenic activity occurring throughout the species’ range of Peru and Chile. Due to recent mining expansions near the species’ largest Peruvian rookery, located at the Punta San Juan (PSJ) reserve, the exposure of breeding individuals to elevated concentrations of trace elements (e.g., heavy metals) in the marine environment is a preeminent concern. While heavy metal toxicity is dependent on metal species, dose, route of exposure, and the physiologic parameters of …

Divergent Metabolic Changes In Rhizomes Of Lowland And Upland Switchgrass (Panicum Virgatum) From Early Season Through Dormancy Onset, Nathan A. Palmer, Gautam Sarath, Michael J. Bowman, Aaron J. Saathoff, Serge J. Edmé, Robert B. Mitchell, Christian M. Tobias, Soundararajan Madhavan, Erin D. Scully, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

High-biomass-yielding southerly adapted switchgrasses (Panicum virgatum L.) frequently suffer from unpredictable winter hardiness at more northerly sites arising from damage to rhizomes that prevent effective spring regrowth. Previously, changes occurring over the growing season in rhizomes sampled from a cold-adapted tetraploid upland cultivar, Summer, demonstrated a role for abscisic acid (ABA), starch accumulation, and transcriptional reprogramming as drivers of dormancy onset and potential keys to rhizome health during winter dormancy. Here, rhizome metabolism of a high-yielding southerly adapted tetraploid switchgrass cultivar, Kanlow—which is a significant source of genetics for yield improvement—was studied over a growing season at a northern …

Identifying Signatures Of Ev Secretion In Metastatic Breast Cancer Through Functional Single-Cell Profiling, Mohsen Fathi, Melisa Martinez-Paniagua, Ali Rezvan, Melisa J Montalvo, Vakul Mohanty, Ken Chen, Sendurai A Mani, Navin Varadarajan

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecules. Despite extensive investigation, heterogeneity in EV secretion among cancer cells and the mechanisms that support EV secretion are not well characterized. We developed an integrated method to identify individual cells with differences in EV secretion and performed linked single-cell RNA-sequencing on cloned single cells from the metastatic breast cancer cells. Differential gene expression analyses identified a four-gene signature of breast cancer EV secretion: HSP90AA1, HSPH1, EIF5, and DIAPH3. We functionally validated this gene signature by testing it across cell lines with different metastatic potential …

A Tool For Selecting Ecological Survey Locations In The Southeastern United States Using A Matched-Paired Design, David R. Nelsen, Aaron G. Corbit, Harvey Alférez

Campus Research Month

Site selection is a critical step for conducting an ecological survey. Inappropriate site selection may lead to confounding variables being included in your data and result in incorrect or inconclusive conclusions ^1,2. The Jorō spider, Trichonephila clavata, is an invasive species ³ that is currently expanding through the Southeaster United States⁴. However, it remains unclear how far the spider will spread and what impact the spider will have on local ecosystems. Early results do suggest that Jorōs are having a negative impact on native orb-weaving spiders⁴, but more systematic research is needed to verify …

Crispr: The Double-Edged Sword Of Genetic Engineering, Grace Spade

Liberty University Research Week

Undergraduate

Three Minute Thesis

Differential Behaviour Of Guineagrass (Panicum Maximum Jacq.) Hybrids, With Different Al+3 Reactions, As To Major Nutrient Translocations To The Leaves, R. S. Paterniani, J. A. Usberti Jr., J. C. Werner

IGC Proceedings (1977-2023)

Most of the Brazilian cultivated pasture fields presents soils with high Al⁺³ levels and liming is economically unfeasible. So, there is an urgent need for grasses with good forage yielding potentials that can withstand Al⁺³ deleterious effects (abnormal root development: short, thick and poorly branched roots, which are unable to effectively translocate water and essential nutrients to the leaves) (Foy, 1984); as a consequence, susceptible genetic materials have their field persistences greatly affected, mainly during drought periods. Researches on Al⁺³ reaction are usually compare supposedly resistant/tolerant genotypes with a resistant control check; doing so, the genotypic effect …

Animal Performance And Productivity Of New Ecotypes Of Brachiaria Brizantha In Brazil, Valéria P. B. Euclides, Manuel C. M. Macedo, Cacilda B. Do Valle, R. Flores, M. P. Oliveira

IGC Proceedings (1977-2023)

Brazil has the competitive advantage of a very dynamic and cost effective animal production system on pastures over other countries. The pursuit for more productive forages that will result in higher quality beef at a lower cost is then justified. Brachiaria is the most important forage genus utilised in Brazil, thus an intense search for new cultivars amongst collected and introduced ecotypes from Africa is underway. Following agronomic evaluation of this material in plots, 8 pre-selected Brachiaria ecotypes were tested under intermittent grazing in paddocks (Euclides et al., 2001). Continuing on the process of cultivar development, two out of …

Development Of A Bahiagrass Paspalum Notatum Flugge With Increased Short-Day Biomass, P. Mislevy, Ann R. Blount, T. R. Sinclair

IGC Proceedings (1977-2023)

Low herbage productivity of subtropical grasses during the short-day winter months of October through to March can place a severe burden on livestock producers in Southeastern U.S. Researchers at the University of Florida (Sinclair et al., 2001) hypothesised that the decrease in forage production might result from physiological dormancy induced by short day length. A study using artificial lights to extend the day length demonstrated that maintaining the day length at 15 hr during the short-day length period increased 'Pensacola' bahiagrass P. notatum Flugge saure Parodi forage yield 122% when compared with normal photoperiod (Mislevy et al., 2001). …

Genetic And Molecular Characterization Of Temperate And Tropical Forage Maize Inbred Lines, B. Alarcón-Zúñiga, E. Valadez-Moctezuma, T. Cervantes-Martinez, T. Cervantes-Santana, M. Mendoza‐Rodriguez

IGC Proceedings (1977-2023)

The livestock feeding in the Central highland of Mexico is based on harvest, grazing and annual forage conservation, being forage maize the most important silage crop (Alarcón, 1995). Even though forage maize is extensively bred in Europe, USA and Asia since 1900's, this started in Mexico in the 1960's, and little is known about the genetic diversity in both agronomic and nutritive value traits. Our breeding program goals are to analyze combining ability of biomass and quality predictors and to study the genetic relationship of inbred lines between lowland tropical and temperate races from Mesa Central, by genetic and molecular …

Preliminary Performance Of Panicum Maximum Accessions And Hybrids In Brazil, Liana Jank, Rosangela M. S. Resende, S. Calixto, M. M. Gontijo Neto, V. A. Laura, Manuel C. M. Macedo, Cacilda B. Do Valle

IGC Proceedings (1977-2023)

Due to the lack of high quality forages adapted to the distinct ecosystems in Brazil, a national network to evaluate 14 accessions, 4 hybrids and 5 commercial standards of Panicum maximum was established in 2002, in 5 regions of Brazil (states of Mato Grosso do Sul, Acre, Rondônia, Minas Gerais and in the Federal District). Staff of the Embrapa Beef Cattle group co-ordinated the network and pre-selected the accessions and hybrids (Jank, 1995; Jank et al., 2001; Resende et al., 2004). We present their performance as to leaf dry matter yield (LDMY) and leaf percentage (LP) obtained from …

Effect Of Applied Biosolids To Bahiagrass Pastures On Copper Status Of Cattle, M. E. Tiffany, L. R. Mcdowell, G. A. O'Connor, N. S. Wilkinson

IGC Proceedings (1977-2023)

When grazing ruminants consume forages high in Mo but adequate in S, there is a risk of molybdenosis (a Mo-induced Cu deficiency). This occurs when Mo, S, and Cu join to form Cu-thiomolybdate complexes in the rumen that are not readily absorbed (Suttle, 1991). High dietary S reduces Cu absorption, possibly due to unabsorbable Cu sulphide formation, independent from its part in thiomolybdate complexes. The use of municipal sludge (biosolids) as a pasture fertiliser is of interest since some contain high Mo which may induce Cu deficiency. The objective of this study was to evaluate the performance and Cu status …

An In Vitro Investigation Of Forage Factors Which Affect The Production Of Conjugated Linoleic Acid And Trans Vaccenic Acid In The Rumen. Ii. Wilting & Cell Damage, Michael R. F. Lee, J. K. S. Tweed, Nigel D. Scollan, R. J. Dewhurst

IGC Proceedings (1977-2023)

Previous studies have shown that cis 9 trans 11 conjugated linoleic acid (CLA) concentrations are higher for summer milk produced from cows grazing fresh pastures than for winter milk when conserved forages are fed (Jahreis et al., 1997). Furthermore, Offer (2003) showed that a similar depression in milk fat CLA occurred if grass was simply cut and fed after a short wilt. This experiment investigated the effect of wilting on the production of CLA and trans vaccenic acid (TVA) in an in vitro rumen simulation, and whether any differences could be related to changes in plant structure. It was …

Effects Of Grassland Management On Herbage Lipid Composition And Consequences For Fatty Acids In Milk, Anjo Elgersma, P. Maudet, I. Witkowska, A. C. Wever

IGC Proceedings (1977-2023)

Herbage provides bulk feed and is the basis for ruminant nutrition. Herbage lipids, especially C18:3, are a major source of beneficial fatty acids (FA) in milk. These desired FA are unsaturated FA such as CLA (conjugated linoleic acid), especially the isomer rumenic acid, and also vaccenic acid, both trans omega-7 FA (Ellen & Elgersma, 2004). As information on lipids in forages is scarce, effects were studied of N application level and regrowth period on the lipid concentration and FA composition of perennial ryegrass (Lolium perenne L.), the most important forage in temperate climate zones. A linear relation had previously …

Effect Of Feeding L-Carnitine And Sunflower Seeds On Cla Content Of Pasture-Fed Beef, S. L. Scott, W. P. Mccaughey, K. E. Buckley

IGC Proceedings (1977-2023)

Pasture finishing enhances levels of conjugated linoleic acids (CLA) in beef lipids (Shanta et al. 1997). CLA (e.g., C18:2 c9, t11), formed during biohydrogenation of polyunsaturated fatty acids (PUFA) in the rumen, can reduce the incidence of heart disease, cancer and obesity in humans. However, pasture finishing cattle can reduce carcass grade. Feeding pasture-fed cattle a high-grain diet for a short finishing period (~60 d) improves grades but may reduce lipid CLA levels. A feeding regime is required that maintains the positive nutritional attributes of pasture-fed beef and improves the meat grade. The objective of this study was to investigate …

Content, Pattern And Esterification Of Fatty Acids In Fresh Grass In Relation To Extraction Solvents And Sample Storage Conditions, Marta Lourenço, Veerle Fievez

IGC Proceedings (1977-2023)

The relatively high content of linolenic acid (C18:3 n-3) in forages has lead to research on the role of forage based feeding strategies in the production of healthier milk. In these studies, forage samples are often stored, although analysis immediately after harvest has been considered to avoid oxidative deterioration and transformation of unsaturated fatty acids (FA) (Christie, 1993; Frankel, 1998). In the current study, we evaluated the effect of different storage conditions (fresh grass samples under liquid nitrogen (liq.N₂) or in a cool box during 3h or at -20ºC, -80ºC or in the extraction solvent at -20°C, during …

Using A Genetic Screen To Discover Gene Functions In Mycobacteriophages Sbash And Island3, Anneka Sterk, Abigail M. Bastian, Sadie A. Gilmeister, Keziah J. Knudson, Erika J. Mckenney, Garrett Raymon, Alaena L. Trevino, April S. Van Tol, Byron Noordewier, Sara S. Tolsma

Celebration of Research

Sbash is a temperate bacteriophage that infects Mycobacterium smegmatis. It was assigned to cluster I2 based on gene-content similarity of 35% or higher to sequenced bacteriophages present in the Actinobacteriophage database, phagesDB. Its genome was annotated in 2014 and found to include 89 protein-coding genes, only 22 of which were assigned functions based on bioinformatic analysis. We are using a genetic screen to identify functions of phage genes for which no function is currently known. We cloned 40 of the genes in Sbash’s genome with sizes ranging from 90 bp to 3,666 bp. We screened each gene for cytotoxicity and …