Life Sciences Commons^™

242 - Validating Zebrafish Chaf1b Antibody, Brianna Donlon, Stephen Welsch, Emma Hatch

GREAT Day Posters

Zebrafish embryos that are homozygous for gef mutant alleles experience a small-eye phenotype, due to a coding change in the chaf1b gene that results in a prematurely truncated protein. It's thought that the Chaf1b protein is required for all dividing cells to survive the DNA replication phase of the cell cycle. Contradicting these findings, the proliferating cells of the gef mutant embryos do not start to prematurely die until they are two days old. One question of delayed death in zebrafish is whether the normal protein can be provided by the heterozygous mother. If so, this would account for how …

256 - Development Of Molecular Markers From The Transcriptome Of An Ant-Mimicking Spider For Population Genetic Analyses, Anna Schell

GREAT Day Posters

The ant-mimicking spider, Myrmarachne formicaria, is a species of jumping spider that is native to Eurasia but has been sighted in North America starting in 2001. This spider is distinctive because of the way it imitates ants by moving its front two legs around like an ant’s antennae. As it is not native to North America, it must have been introduced to the country, but there is not currently enough information to determine whether there was only one introduction event or several different events. We can distinguish between these scenarios and learn more about this spider's dispersal history by analyzing …

345 - The Effects Of Alcohol And Ketogenic Diet On Working Memory In Mice, Kaitlyn Dufresne, Sophia Azurin, Allison Bechard

GREAT Day Posters

The ketogenic diet (KD) is a high fat, low carb diet which is growing in popularity as a possible method to improve working memory and prevent alcohol use disorder (AUD). We used a mouse model where half of the mice were fed a standard diet (SD) while the other half were fed the KD. All mice experienced a 7 week drinking period where they were introduced to alcohol for 2 hours, 3 days a week. We then tested the mice in a working memory task. We modeled working memory using the Barnes maze. Mice escaped to a target hole in …

116 - The Effects Of Tetrahydrocannabinol On Circadian Rhythmicity And The Response To Nicotine In Long-Evans Rats, Lauren Mac Taggart, Anna Hendricks, Joey Morgan, Vincent Markowski

GREAT Day Posters

The marijuana legalization trend promotes the notion that cannabinoids such as tetrahydrocannabinol (THC) can be safe and effective medications for conditions such as insomnia. Unfortunately, exposure to THC during development has been shown to impair cognition and motor behavior in humans and laboratory animals. The current study used VitalView software and cage-top infrared activity monitors to quantify the effects of a range of THC doses on activity and circadian rhythms in adult rats that were exposed to THC throughout adolescence. Rats were further administered acute nicotine drug challenges to determine if THC altered the development of the acetylcholine neurotransmitter system. …

122 - Using Crispr/Cas9 Genome Editing To Knockout Mhc Class I In Xenopus Laevis, Keely Glasheen, David Marx, Jacques Robert

GREAT Day Posters

The immune system of the frog Xenopus laevis is similar to humans. MHC Class I is a vital molecule for the immune system of Xenopus laevis. It presents peptides to CD8 T-cells and the presentation of self peptide fragments is crucial for immune self recognition. When MHC Class I presents non-self peptide fragments, it triggers an immune response, causing CD8 T-cells to kill the infected cells. All cells express some level of MHC Class I because all cells can be infected. The role this molecule plays in immune function and self recognition is of particular interest in Xenopus laevis since …

126 - The Effects Of Perinatal Or Peripubertal Exposure To Tetrahydrocannabinol On Developmental Milestones, Motor Behavior, And Somatosensation In Rats, Anna Hendricks, Lindsey Toole, Allyson Surowick, Danielle Roemer, Vincent Markowski

GREAT Day Posters

Tetrahydrocannabinol (THC) is the primary psychoactive agent in marijuana, one of the most popular recreational drugs in the world. As the legalization trend in the U.S. expands, more women are consuming THC during pregnancy and more teens are experimenting with the drug. Unfortunately, the effects of THC on fetal and adolescent brain development are poorly understood. The current study was designed to identify the developmental and somatomotor effects of THC exposure. Long-Evans rats were assigned to one of two cohorts: prenatal exposure where pregnant rats were administered THC from gestational day 1 to postnatal day 21 or juvenile exposure where …

129 - Analysis Of Corpus Callosum And Ventricles In Brain Tissue Of Mk801/Cdppb Treated C57bl6/J Mice, Ryan Pelkey, Noah Massey, Evan Eshenaur, Zander Purcell, Vincent Markowski

GREAT Day Posters

The neurodevelopmental hypothesis depicts schizophrenia as a long-term consequence of aberrant development of the glutamate and dopamine neurotransmitter systems during the perinatal period. The drug MK-801 is a glutamate N-methyl-D-aspartate (NMDA) receptor antagonist that produces schizophrenia-like symptoms in laboratory rodents when administered early in development. Our laboratory has been investigating whether CDPPB, a positive allosteric modulator of the metabotropic glutamate receptor 5 (mGluR5), would reverse the effects of MK-801. To test this, mice were first administered 0.25 mg/kg MK-801 as neonates, and later given 10.0 mg/kg CDBBD or saline during their juvenile period. Previously, our lab found that MK-801 produced …

146 - Localization Of Sonic Hedgehog (Shh) Protein In Zebrafish, Muwafuq Ibrahim, Jacqueline Maloney, Christopher Jung, Zee Ralph, Isabell Mathew

GREAT Day Posters

Zebrafish possess a unique ability to regenerate their retina when damaged; our objective is to gain a better understanding regarding retinal regeneration in zebrafish in hopes of eventually utilizing this information to promote retinal regeneration in humans. It has been shown that a protein, Sonic Hedgehog signaling protein (shh), plays a role in the differentiation of progenitor cells during eye development in zebrafish. Researchers have concluded that shh moves across the retina in a wave-like fashion during vertebrate development and regeneration. Studies have been conducted to see how the shh protein moves, but none have investigated the exact location that …

162 - The Importance Of Chaf-1b And P53 In Zebrafish Apoptosis, Noé Stephens, Dhavan Brahmbhatt, Isabel Reitano-Stayer, Hannah Haws

GREAT Day Posters

Chromatin assembly factor-1b (Caf-1b), one of the subunits of Chromatin assembly factor-1, is integral for retina differentiation in both humans and zebrafish. Fisher et. al found that apoptosis was stimulated in cells that lack Caf-1b at the S-phase check point by the tumor suppressor p53 as cells that lacked Caf-1b had a much higher expression of p53 then cells that contained Caf-1b. Fisher et. al proposed the model that Caf-1b is an inhibitor of p53 which stimulates apoptosis in the absence of Caf-1b (2007). However, it is not known what occurs when both Caf-1b and p53 are both removed. If …

178 - Ketogenic Diet And Social Behavior In A Mouse Model Of Autism Spectrum Disorder, Macy Kuebler, Abigail Grzeskowiak, Emily Trabulsi, Abigail Schwartzman, Gabrielle Skiba

GREAT Day Posters

Impaired social interaction is one of three key diagnostic criteria for Autism Spectrum Disorder (ASD). Other criteria for ASD include repetitive behavior and impaired communication skills. This developmental condition is increasing within the United States, yet no cure is currently available. Ketogenic diet (KD) is a high fat, low carb diet proven to help many neurological issues in humans and reduce repetitive behavior in the mouse model. This study uses a FVB mouse model predisposed to developing stereotypical behaviors, specifically, repetitive circling to investigate the effects of KD on social and repetitive behavior. Subjects were old and young mice fed …

204 - How Does Change In Land Use Impact The Water Chemistry Parameters In The Streams Of Oswego County, Ny, Madison Steates, Thomas Back

GREAT Day Posters

Land use within a watershed is closely tied to stream water chemistry. Anthropogenic chemical inputs eventually make their way into streams, affecting fluvial ecosystems. Sources of these anthropogenic inputs change over time, especially when rural landscapes become increasingly urbanized. We studied the relationship between land use and stream water chemistry for Oswego County, New York, because riparian zones in this county have shifted to residential from agricultural and forested land uses. We extracted data from the New York State Department of Conservation (DEC) Department of Water (DOW) Monitoring Portal. Preliminary results show that Total Nitrogen (Kjeldahl) in mg/L fluctuates yearly …

237 - Seasonal And Historical Trends Of Cyanobacteria Dominance In Conesus Lake, Emma Belica, Isabella Imbo

GREAT Day Posters

The purpose of this research was three-fold. First, an analysis of long-term data was conducted to determine how the abundance of the cyanobacteria of the genus Dolichospermum changed historically since 1985 in Conesus Lake. Secondly, to look at the seasonality of the Dolichospermum blooms in the Summer of 2022. Third, to determine Dolichospermum's Nitrogen-fixing activity based on analysis of the frequency of Nitrogen-fixing cells in the population (heterocysts). Long-term data shows Dolichospermum has increased in representation in recent years and in 2022 reached a peak biomass that represented 75% of all the cyanobacteria biomass and 56% of all the phytoplankton …

245 - P27kip1 Is Upregulated In Umscv-4 Cells Corresponding To Entering A State Of Quiescence When Treated With Clobetasol., Gianna Minnuto, Luke North, Mack Ogden, Kia Haering

GREAT Day Posters

Quiescence, the temporary exit from the cell cycle, presents therapeutic challenges to cancer management since it allows evasion of chemotherapy and radiotherapy treatment. Essential to the study of quiescence in carcinogenesis is an established model system. Our studies have found that clobetasol treatment of the vulvar cancer cell line, UMSCV-4 causes these cells to enter a state of dormancy. Subsequent removal of the cells from clobetasol show a return to normal cell proliferation, even after dormancy for 3 months. p27^Kip1 protein is elevated in quiescent cells and has been used as a marker for entry into quiescence. Using both …

352 - Quantifying Variation Of The Ostrich Wing (Aves: Paleognathae), Isabel Marzec

GREAT Day Posters

The common Ostrich (Struthio camelus) is a member of a large clade of flightless birds known as the ratites. The ratites are morphologically classified by their absence of a keeled sternum that, in flighted birds, provides a large surface area for flight muscle attachment. Although morphology in flighted birds is relatively constrained, the lack of selective pressures in ratites has led to increased variability in the wing. The skeleton of S. camelus exhibits numerous adaptations related to cursoriality, such as a double patella, shortened wing skeleton, and a lightweight pelvic girdle. Though their wings may not be capable …

The Role Of Cerium(Iii) In Bacterial Growth And The Microbial Transformation Of Aromatic Hydrocarbons, Shruti Sathish

Honors Theses

Biofilms are communities of surface-attached bacterial cells encased in an exopolymeric matrix. In this state, they are more resistant to antimicrobial treatment and can have adverse effects in medical, agricultural, and industrial settings. Whereas, as biocatalysts, biofilms from nonpathogenic bacteria enhance their performance and stability in catalysis. Unfortunately, there are several challenges when using bacteria in organic transformations due to their complex cellular chemistry. Trivalent lanthanide metals were discovered to serve regulatory roles in some bacterial catalytic processes, including those of Pseudomonas putida KT2440 (P. putida), a non-infectious Gram-negative bacterium. The main goal of our research is to use cerium(III) …

Wellness Review 2022, Part 2, Martin Huecker, Brian A. Ferguson, Jacob Shreffler

Journal of Wellness

Introduction: Similar to prior reviews, the Journal of Wellness editors searched the literature from the second half of 2022 for an interesting and impactful selection of publications on wellness in healthcare professionals.

Methods: Editors conducted a standard keyword search in Pubmed, focusing chiefly on large journals, interventional trials, and other prospective research. We included papers published between July 1, 2022 and December 31, 2022.

Literature in Review: Finding several hundred publications, we excluded editorials, reviews, and some smaller, less generalizable papers. A final 25 significant studies focusing on wellness in medical professionals were sorted into the following categories: logistics, creativity …

An Ecological Assessment Of The Deep-Pelagic Fish Genus Cyclothone (Gonostomatidae; Stomiiformes), Possibly The World’S Most Abundant Fishes, In The Gulf Of Mexico, Olivia C. North-Menthonnex

All HCAS Student Capstones, Theses, and Dissertations

The fish genus Cyclothone is considered the most abundant vertebrate taxon on Earth. Despite this assertion, very few detailed ecological studies of this genus exist for any site in the World Ocean, largely due to the lack of expertise (and willingness) to identify specimens from existing sample sets. This study will provide a species-level description of the abundance, vertical distribution, and size structuring of the genus Cyclothone in the Gulf of Mexico, a hyper-diverse, deep-pelagic ecosystem that is increasingly impacted by anthropogenic disturbances. As the putative most-abundant fishes in the ecosystem, this characterization is critically needed for a holistic understanding …

Hica Toxin-Based Counterselection Marker For Allelic Exchange Mutations In Fusobacterium Nucleatum, Bibek Gc, Peng Zhou, Chenggang Wu

Faculty, Staff and Student Publications

The study of fusobacterial virulence factors has dramatically benefited from the creation of various genetic tools for DNA manipulation, including galK-based counterselection for in-frame deletion mutagenesis in Fusobacterium nucleatum, which was recently developed. However, this method requires a host lacking the galK gene, which is an inherent limitation. To circumvent this limitation, we explored the possibility of using the hicA gene that encodes a toxin consisting of a HicAB toxin-antitoxin module in Fusobacterium periodonticum as a new counterselective marker. Interestingly, the full-length hicA gene is not toxic in F. nucleatum, but a truncated hicA gene version lacking the first …

Effectors And Effects Of Arginine Methylation, Yalong Wang, Mark T Bedford

Faculty, Staff and Student Publications

Arginine methylation is a ubiquitous and relatively stable post-translational modification (PTM) that occurs in three types: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Methylarginine marks are catalyzed by members of the protein arginine methyltransferases (PRMTs) family of enzymes. Substrates for arginine methylation are found in most cellular compartments, with RNA-binding proteins forming the majority of PRMT targets. Arginine methylation often occurs in intrinsically disordered regions of proteins, which impacts biological processes like protein-protein interactions and phase separation, to modulate gene transcription, mRNA splicing and signal transduction. With regards to protein-protein interactions, the major 'readers' of methylarginine marks are …

A Deep Learning Framework For Processing And Classification Of Hyperspectral Rice Seed Images Grown Under High Day And Night Temperatures, Víctor Díaz-Martínez, Jairo Orozco-Sandoval, Vidya Manian, Balpreet K. Dhatt, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A framework combining two powerful tools of hyperspectral imaging and deep learning for the processing and classification of hyperspectral images (HSI) of rice seeds is presented. A seed-based approach that trains a three-dimensional convolutional neural network (3D-CNN) using the full seed spectral hypercube for classifying the seed images from high day and high night temperatures, both including a control group, is developed. A pixel-based seed classification approach is implemented using a deep neural network (DNN). The seed and pixel-based deep learning architectures are validated and tested using hyperspectral images from five different rice seed treatments with six different high temperature …

An Investigation Of Second-Site Non-Complementation Among Motile Cilia Genes In Chlamydomonas Reinhardtii, Gervette M. Penny

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATION An Investigation of Second-Site Non-Complementation among Motile Cilia Genes in Chlamydomonas reinhardtii by Gervette M. Penny Doctor of Philosophy in Biology and Biomedical Sciences Molecular Genetics and Genomics Washington University in St. Louis, 2023 Professor Susan K. Dutcher, Chair Motile cilia are complex microtubule-based organelles used by eukaryotic cells for locomotion or directing fluid flow. In humans, motile cilia defects cause primary ciliary dyskinesia (PCD), characterized by neonatal respiratory distress, recurrent lung infections, bronchiectasis, and low nitric oxide. Situs inversus and/or male infertility are present in about 50% of cases. PCD inheritance is recessive; both copies …

Overcoming Adaptive Resistance To Anti-Vegf Therapy By Targeting Cd5l, Christopher J Lafargue, Paola Amero, Kyunghee Noh, Lingegowda S Mangala, Yunfei Wen, Emine Bayraktar, Sujanitha Umamaheswaran, Elaine Stur, Santosh K Dasari, Cristina Ivan, Sunila Pradeep, Wonbeak Yoo, Chunhua Lu, Nicholas B Jennings, Vinod Vathipadiekal, Wei Hu, Anca Chelariu-Raicu, Zhiqiang Ku, Hui Deng, Wei Xiong, Hyun-Jin Choi, Min Hu, Takae Kiyama, Chai-An Mao, Rouba Ali-Fehmi, Michael J Birrer, Jinsong Liu, Ningyan Zhang, Gabriel Lopez-Berestein, Vittorio De Franciscis, Zhiqiang An, Anil K Sood

Faculty, Staff and Student Publications

Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) pathway is a powerful tool to combat tumor growth and progression; however, drug resistance frequently emerges. We identify CD5L (CD5 antigen-like precursor) as an important gene upregulated in response to antiangiogenic therapy leading to the emergence of adaptive resistance. By using both an RNA-aptamer and a monoclonal antibody targeting CD5L, we are able to abate the pro-angiogenic effects of CD5L overexpression in both in vitro and in vivo settings. In addition, we find that increased expression of vascular CD5L in cancer patients is associated with bevacizumab resistance and worse overall survival. …

Bridging Clinic And Wildlife Care With Ai-Powered Pan-Species Computational Pathology, Khalid Abduljabbar, Simon P Castillo, Katherine Hughes, Hannah Davidson, Amy M Boddy, Lisa M Abegglen, Lucia Minoli, Selina Iussich, Elizabeth P Murchison, Trevor A Graham, Simon Spiro, Carlo C Maley, Luca Aresu, Chiara Palmieri, Yinyin Yuan

Faculty, Staff and Student Publications

Cancers occur across species. Understanding what is consistent and varies across species can provide new insights into cancer initiation and evolution, with significant implications for animal welfare and wildlife conservation. We build a pan-species cancer digital pathology atlas (panspecies.ai) and conduct a pan-species study of computational comparative pathology using a supervised convolutional neural network algorithm trained on human samples. The artificial intelligence algorithm achieves high accuracy in measuring immune response through single-cell classification for two transmissible cancers (canine transmissible venereal tumour, 0.94; Tasmanian devil facial tumour disease, 0.88). In 18 other vertebrate species (mammalia = 11, reptilia = 4, aves …

Nesting Success Of American Robins (Turdus Migratorius) In Suburban Areas Of The Arkansas River Valley, Brandon Maiersperger

ATU Scholars Symposium

The American Robin (Turdus migratorius) is an abundant North American songbird species that thrives in suburban areas. Estimates of robin nesting success in suburban areas range from 31% to 90%. Robin nest site selection and success have not received much attention in the past few decades. Most passerines have a balanced sex ratio at fledging, but little is known about the fledgling sex ratio of robins. We located 44 robin nests in six public parks around Russellville, AR and on the Arkansas Tech University campus. Nest success was low; 27% (12/44) of nests fledged at least one young. Robins nested …

Antibacterial Properties Of Devil’S Walking Stick And Winged Sumac Extracts, Raven L. Turner, Kayla L. Medina

ATU Scholars Symposium

Many native plants are used for the treatment of various diseases. Mainly those species in high chemical compound plant families can have antimicrobial properties. We selected two native plants in Arkansas, Devil’s walking stick (Aralia spinosa), and Winged sumac (Rhus copallinum), and tested them for antibacterial properties. We used three gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three gram-negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion method is employed to identify any potential antibacterial properties for the two plant species. For this experiment, 6.50 g of dehydrated plant material (leaves of each plant …

Acoustic Sampling Of Arkansas Bat Species Richness Across Different Seasons And Habitats, Lark E. Sybrant

ATU Scholars Symposium

Arkansas is home to sixteen bat species belonging to the Vespertilionidae and Molossidae families. Bats are keystone species and provide numerous ecosystem services that also benefit people. This study explores factors that impact species richness between the spring and fall seasons in a wooded rural site (Washburn Park) and an urban field site (Arkansas Tech University campus), Russellville, AR. The non-invasive Echo Meter Touch 2 Pro, from Wildlife Acoustics, was used to record calls and identify bat species from these locations. Nearly 1,050 calls were recorded from 11 different species across the spring and fall between both sites. Results showed …

White Blood Cell Make Up In Kellback Snake Offspring Of Varying Maternal Size, Angelica Mccoy

Symposium of Student Scholars

The immune system is composed of various organs, systems, and other components with one of the most important being white blood cells. White blood cells are commonly separated into two scientific varieties, granulocytes and agranulocytes, which can be differentiated by the presence or absence of granules in the cytoplasm once stained on a slide. There are three granulocytes and two agranulocytes, each with a distinct function and shape. The ratio of these may impact the effectiveness of this portion of the immune system, and multiple factors could play a role in this ratio, an example being the size of an …

Does A Brisk Six Minute Walk Cause Upper Body Fatigue In Females, Kaden Buford

Symposium of Student Scholars

Non-local performance fatigue (NLPF) can be described as performance decrements occurring for a muscle group that was not directly involved in the fatiguing activity that preceded. Most studies on NLPF involve strenuous exercise of an isolated joint in trained populations, however, little evidence exists on NLPF derived from activities mimicking acts of daily living. Determining whether NLPF exists following brisk walking is worthwhile, and rate of force development (RFD), the rate at which muscle force is produced, may possess increased susceptibility compared to maximal strength. The purpose of our ongoing study is to determine the responses, if any, for maximal …

Distribution Of Micropredators In The Longleaf Pine Soil Microbiome, Faith Arends, Eithar Mirghani

Symposium of Student Scholars

Bacterial micropredators of the soil, like their larger-scale counterparts, play a key role in shaping their ecosystem and are thus of particular interest from an ecological standpoint. An important micropredator to study are Myxobacteria. Myxobacteria are gram-negative rod-shaped social bacteria that hunt in wolf packs to lyse their prey and facilitate their growth. Myxobacteria are a potential source of novel antibiotics, therefore, the isolation of new strains is of great interest. We investigated longleaf pine soil as a source of novel isolates. The Longleaf Pine Ecosystem is an endangered ecosystem that is of crucial importance due to its ability to …

Antibacterial Activity Of Native Plants In Arkansas, Kayla L. Medina, Raven L. Turner, Katie Huffman

ATU Scholars Symposium

American beautyberry (Callicarpa Americana), Sugarberry (Celtis laevigata) and Red buckeye (Aesculus pavia) are native plants of Arkansas. Plant families with species having high chemical compounds can have antimicrobial properties. In this study, the antibacterial properties of the plants are investigated against three gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion assay method is employed to identify any potential antibacterial properties of the plants. For this experiment, 6.41 g of dehydrated plant material (leaves for both species) was combined with 50 mL of 75% ethanol creating …