Life Sciences Commons^™

Secondary Validation Of An Ovarian Cancer-Specific Comorbidity Index In A Us Population, Chelsey Vranes, Hui Zhao, Mette Calundann Noer, Shuangshuang Fu, Charlotte C Sun, Ross Harrison, Pedro T Ramirez, Claus Kim Høgdall, Sharon H Giordano, Larissa A Meyer

Faculty, Staff and Student Publications

Objectives: The Ovarian Cancer Comorbidity Index (OCCI) is an age-specific index developed and previously found to be more predictive of overall and cancer-specific survival than the Charlson Comorbidity Index (CCI). The objective was to perform secondary validation of the OCCI in a US population.

Methods: A cohort of ovarian cancer patients undergoing primary or interval cytoreductive surgery from January 2005 to January 2012 was identified in SEER-Medicare. OCCI scores were calculated with the regression coefficients determined from the original developmental cohort for five comorbidities. Cox regression analyses were used to calculate associations between the OCCI risk groups and 5-year overall …

Understanding Cardiac Senescence One Cell Type At A Time, Payel Sen, Priyatansh Gurha

Faculty, Staff and Student Publications

No abstract provided.

Nonlinear Propagation Of Quasiplanar Shear Wave Beams In Soft Elastic Media With Transverse Isotropy, John M Cormack, Mark F Hamilton

Faculty, Staff and Student Publications

Model equations are developed for shear wave propagation in a soft elastic material that include effects of nonlinearity, diffraction, and transverse isotropy. A theory for plane wave propagation by Cormack [J. Acoust. Soc. Am. 150, 2566 (2021)] is extended to include leading order effects of wavefront curvature by assuming that the motion is quasiplanar, which is consistent with other paraxial model equations in nonlinear acoustics. The material is modeled using a general expansion of the strain energy density to fourth order in strain that comprises thirteen terms defining the elastic moduli. Equations of motion for the transverse displacement components are …

Pirtobrutinib And Venetoclax Combination Overcomes Resistance To Targeted And Chimeric Antigen Receptor T-Cell Therapy In Aggressive Mantle Cell Lymphoma, Yang Liu, Fangfang Yan, Vivian Changying Jiang, Yijing Li, Yuxuan Che, Joseph Mcintosh, Alexa Jordan, Ian Hou, Lei Nie, Jingling Jin, Wei Wang, Heng-Huan Lee, Yixin Yao, Michael Wang

Faculty, Staff and Student Publications

No abstract provided.

Vestigial-Like 1 (Vgll1): An Ancient Co-Transcriptional Activator Linking Wing, Placenta, And Tumor Development, Heather M Sonnemann, Barbara Pazdrak, Dinler A Antunes, Jason Roszik, Gregory Lizée

Faculty, Staff and Student Publications

Vestigial-like 1 (VGLL1) is a recently discovered driver of proliferation and invasion that is expressed in many aggressive human malignancies and is strongly associated with poor prognosis. The VGLL1 gene encodes for a co-transcriptional activator that shows intriguing structural similarity to key activators in the hippo pathway, providing important clues to its functional role. VGLL1 binds to TEAD transcription factors in an analogous fashion to YAP1 but appears to activate a distinct set of downstream gene targets. In mammals, VGLL1 expression is found almost exclusively in placental trophoblasts, cells that share many hallmarks of cancer. Due to its role as …

Falciform Ligament Hernia: Combination Of Key Ct Findings, Tomonori Matsuura, Nozomi Satani, Ryo Tamura, Yuriko Kagaya, Takayuki Yamada

Faculty, Staff and Student Publications

A male patient in his early 90s with no history of abdominal surgery was referred to us for abdominal pain and vomiting. An abdominal computed tomography (CT) demonstrated dilated small bowel with a double beak sign and poorly enhanced wall, which indicated a closed-loop obstruction that leads to strangulation. A closed-loop bowel was located in front of the anterior and medial segments of the liver and to the right of the round ligament of the liver on axial images. Sagittal images revealed that the round ligament has deviated downward and 2 adjacent narrowed intestines were located at its cranial side. …

Multi-Institutional Study Of Pathologist Reading Of The Programmed Cell Death Ligand-1 Combined Positive Score Immunohistochemistry Assay For Gastric Or Gastroesophageal Junction Cancer, Aileen I Fernandez, Charles J Robbins, Patricia Gaule, Diana Agostini-Vulaj, Robert A Anders, Andrew M Bellizzi, Wei Chen, Zongming Eric Chen, Purva Gopal, Lei Zhao, Mikhail Lisovsky, Xiuli Liu, Jinru Shia, Huamin Wang, Zhaohai Yang, Leena Mccann, Yvonne G Chan, Jodi Weidler, Michael Bates, Xuchen Zhang, David L Rimm

Faculty, Staff and Student Publications

The assessment of the expression of programmed cell death ligand-1 (PD-L1) using immunohistochemistry (IHC) has been controversial since its introduction. The methods of assessment and the range of assays and platforms contribute to confusion. Perhaps the most challenging aspect of PD-L1 IHC is the combined positive score (CPS) method of interpretation of IHC results. Although the CPS method is prescribed for more indications than any other PD-L1 scoring system, its reproducibility has never been rigorously assessed. In this study, we collected a series of 108 gastric or gastroesophageal junction cancer cases, stained them using the Food and Drug Administration-approved 22C3 …

Publisher Correction: Comprehensive Molecular Characterization Of Mitochondrial Genomes In Human Cancers, Yuan Yuan, Young Seok Ju, Youngwook Kim, Jun Li, Yumeng Wang, Christopher J Yoon, Yang Yang, Inigo Martincorena, Chad J Creighton, John N Weinstein, Yanxun Xu, Leng Han, Hyung-Lae Kim, Hidewaki Nakagawa, Keunchil Park, Peter J Campbell, Han Liang, Pcawg Consortium

Faculty, Staff and Student Publications

No abstract provided.

Neddylation Inhibition Sensitises Renal Medullary Carcinoma Tumours To Platinum Chemotherapy, Daniel D Shapiro, Niki Millward Zacharias, Durga N Tripathi, Menuka Karki, Jean-Philippe Bertocchio, Melinda Soeung, Rong He, Mary E Westerman, Jianjun Gao, Priya Rao, Truong N A Lam, Eric Jonasch, Luigi Perelli, Emily H Cheng, Alessandro Carugo, Timothy P Heffernan, Cheryl L Walker, Giannicola Genovese, Nizar M Tannir, Jose A Karam, Pavlos Msaouel

Faculty, Staff and Student Publications

BACKGROUND: Renal medullary carcinoma (RMC) is a highly aggressive cancer in need of new therapeutic strategies. The neddylation pathway can protect cells from DNA damage induced by the platinum-based chemotherapy used in RMC. We investigated if neddylation inhibition with pevonedistat will synergistically enhance antitumour effects of platinum-based chemotherapy in RMC.

METHODS: We evaluated the IC50 concentrations of the neddylation‐activating enzyme inhibitor pevonedistat in vitro in RMC cell lines. Bliss synergy scores were calculated using growth inhibition assays following treatment with varying concentrations of pevonedistat and carboplatin. Protein expression was assessed by western blot and immunofluorescence assays. The efficacy of pevonedistat …

Evaluation Of The Impact Of Formaldehyde Fumigation During The Hatching Phase On Contamination In The Hatch Cabinet And Early Performance In Broiler Chickens, C. M. Selby, L. C. Beer, A. J. Forga, M. E. Coles, L. E. Graham, K. D. Teague, G. Tellez-Isaias, B. M. Hargis, C. N. Vuong, B. D. Graham

Poultry Science Faculty Publications and Presentations

Commercial hatch cabinet environments promote replication of microorganisms. These pathogenic or apathogenic microorganisms may serve as pioneer colonizers of the gastrointestinal tract (GIT) of poultry. Some of these pioneer colonizers, such as Escherichia coli and Enterococcus spp., are opportunistic pathogens that lead to reduced performance in commercial poultry. Effective hatchery sanitation is imperative to limit contamination of naïve neonatal chicks and poults. Formaldehyde fumigation has been traditionally used to reduce the pathogen load in commercial hatch cabinets. To investigate potential alternatives to formaldehyde fumigation, models to mimic the microbial bloom in a laboratory setting must be utilized. The …

Bone Metabolite Profile Differs Between Normal And Femur Head Necrosis (Fhn/Bco)-Affected Broilers: Implications For Dysregulated Metabolic Cascades In Fhn Pathophysiology, Alison Ramser, Rachel Hawken, Elizabeth Greene, Ron Okimoto, Brenda Flack, Courtney J. Christopher, Shawn R. Campagna, Sami Dridi

Poultry Science Faculty Publications and Presentations

Femur head necrosis (FHN), also known as bacterial chondronecrosis with osteomyelitis (BCO), has remained an animal welfare and production concern for modern broilers regardless of efforts to select against it in primary breeder flocks. Characterized by the bacterial infection of weak bone, FHN has been found in birds without clinical lameness and remains only detectable via necropsy. This presents an opportunity to utilize untargeted metabolomics to elucidate potential non-invasive biomarkers and key causative pathways involved in FHN pathology. The current study used ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC–HRMS) and identified a total of 152 metabolites. Mean intensity …

Assessment Of Meat Quality Attributes Of Four Commercial Broiler Strains Processed At Various Market Weights, A. J. Mueller, C. J. Maynard, A. R. Jackson, M. T. Kidd, S. J. Rochell, J. P. Caldas-Cueva, X. Sun, A. Giampietro-Ganeco, C. M. Owens

Poultry Science Faculty Publications and Presentations

As the demand for poultry meat continues to rise, industry production is constantly challenged with obtaining consumer needs. Integrators have answered this increasing demand by improving the growth rate of broilers allowing for increased production efficiently. The resulting broiler produces higher yields and a larger quantity of fresh poultry to satisfy consumer needs. However, this increase in efficiency has cost integrators as new quality issues continue to manifest through global production. Therefore, the objective of the current experiment was to evaluate the effect of genetic strain (standard and high yielding) and target weight on meat quality attributes such as pH, …

The Effects Of Essential Oil From Lippia Origanoides And Herbal Betaine On Performance, Intestinal Integrity, Bone Mineralization And Meat Quality In Broiler Chickens Subjected To Cyclic Heat Stress, Roberto Señas-Cuesta, Andressa Stein, Juan D. Latorre, Clay J. Maynard, Xochitl Hernandez-Velasco, Victor Petrone-Garcia, Elizabeth Greene, Makenly Coles, Latasha Gray, Lauren Laverty, Kristen Martin, Ileana Loeza, Alvaro J. Uribe, Blanca C. Martinez, Jaime A. Angel-Isaza, Danielle Graham, Casey M. Owens, Billy M. Hargis, Guillermo Tellez-Isaias

Poultry Science Faculty Publications and Presentations

Essential oils (EO) affect performance, intestinal integrity, bone mineralization, and meat quality in broiler chickens subjected to cyclic heat stress (HS). Day-of-hatch Cobb 500 male broiler chicks (n = 475) were randomly divided into four groups. Group 1: No heat stress (Thermoneutral) + control diets with no antibiotics; Group 2: heat stress control + control diets; Group 3: heat stress + control diets supplemented with thymol chemotype (45 ppm) and herbal betaine (150 ppm) formulation EO1; Group 4: heat stress + control diets supplemented with phellandrene (45 ppm) and herbal betaine (150 ppm) formulation EO2. From day 10–42, the …

An Evaluation Of The Thermoregulatory Potential Of In Ovo Delivered Bioactive Substances (Probiotic, Folic Acid, And Essential Oil) In Broiler Chickens, Samson Oladokun, Sami Dridi, Deborah Adewole

Poultry Science Faculty Publications and Presentations

Mitigating the negative effects of heat stress (HS) is a critical challenge for the global poultry industry. This study evaluated the thermoregulatory potential of 3 in ovo delivered bioactive substances using selected gut health parameters. Eggs were incubated and allotted to 5 groups, and respective bioactive substances delivered. These groups included—the noninjected, in ovo saline, in ovo folic acid (FA), in ovo probiotics (P), and in ovo essential oil (EO). At hatch, chicks were assigned to 5 new posthatch treatment combinations, including A) Negative control (NC)—noninjected eggs offered a basal …

Meat Quality Attributes Of Male And Female Broilers From 4 Commercial Strains Processed For 2 Market Programs, C. J. Maynard, A. R. Jackson, J. P. Caldas-Cueva, A. Mauromostakos, M. T. Kidd, S. J. Rochell, C. M. Owens

Poultry Science Faculty Publications and Presentations

In recent times, meat quality has become a key aspect of poultry production. In the current study, 2,400 sex separate broilers from 4 commercial strains were placed in 6 replications to evaluate various meat quality characteristics when grown to 2 market weights. Broilers were fed 1 of 2 diets with varying degrees of amino acid inclusion to evaluate meat quality of broiler raised on varying planes of nutrition. Birds were processed to meet 2 specified target weights (2.5 and 3.8 kg) representing small bird and big bird debone markets in the United States, respectively. Birds were processed using commercial methods, …

A Comparison Of External Loads In Division Iii Men's Lacrosse Between High Competition Matches And Low Competition Matches, Patrick Moore

Undergraduate Theses and Capstone Projects

Lacrosse is an open field sport with limited knowledge of the physiological demands of gameplay at the Division III level. The purpose of this study was to investigate the external loads of Division III men’s lacrosse players during NCAA season games. Comparisons were made between the external loads placed on the athletes in high competition versus external loads placed on the athletes in low competition matches. Top competition matches were defined as matches against teams that qualified for the NCAA tournament whereas low competition matches included teams that did not meet top competition requirements. The dependent variables measured included total …

Peers And The Choice Of Irrigation Practices In The Arkansas Delta, Kent Kovacs, Christopher Henry, Qiuqiong Huang, Larry Jason Krutz

Agricultural Economics and Agribusiness Faculty Publications and Presentations

There are a range of demographic, climatic, and economic factors that influence the use of irrigation practices, along with the effect of peers and Extension agents on agricultural technologies. This research was conducted to determine how a peer's use of multiple irrigation practices influences the use of common irrigation practices in the Delta region of Arkansas. Using data from a producer survey conducted by telephone in 2015, we identify the determinants of the use and the share of an irrigated farm with five common irrigation practices in the Arkansas Delta. The role of peers for irrigation practice use depends on …

Microbial Associations With Metcalf's Tryonia, Tryonia Metcalfi (Gastropoda: Cochliopidae), An Imperiled Ciénega Endemic, Russell L. Minton, Kathryn E. Perez

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The Chihuahuan Desert swamps are a hotspot for imperiled organisms including freshwater springsnails and bacteria. Many of these taxa are endemic to the desert and to the individual waterbodies where they occur. Efforts to conserve diversity in these threatened areas must account for the life history of the organisms, but also interactions between organisms including microbes. We documented the microbial assemblage associated with Tryonia metcalfi, a critically imperiled freshwater snail endemic to a ciénega system in western Texas. We identified 14 bacterial families in our snail samples and determined a core assemblage of 19 bacterial taxa (4 of which …

A Comprehensive Analysis Of Different Types Of Databases Reveals That Cdh1 Mrna And E-Cadherin Protein Are Not Downregulated In Most Carcinoma Tissues And Carcinoma Cell Lines, Brihget Sicairos, Shorna Alam, Yuchun Du

Biological Sciences Faculty Publications and Presentations

Background

The CDH1 gene codes for the epithelial-cadherin (E-cad) protein, which is embedded in the plasma membrane of epithelial cells to form adherens junctions. E-cad is known to be essential for maintaining the integrity of epithelial tissues, and the loss of E-cad has been widely considered a hallmark of metastatic cancers enabling carcinoma cells to acquire the ability to migrate and invade nearby tissues. However, this conclusion has come under scrutiny.

Methods

To assess how CDH1 and E-cad expression changes during cancer progression, we analyzed multiple large transcriptomics, proteomics, and immunohistochemistry datasets on clinical cancer samples and cancer cell lines …

Dopamine Dysregulation In Antiretroviral Drugs Treated Neuronal Cells, Andrea S. Bustos

Theses and Dissertations

According to the world health organization (WHO), 37.7 million people worldwide in 2020 were living with the human immunodeficiency virus (HIV-1). In the US, combination antiretroviral therapy (cART) drugs have shown effectiveness in combating HIV-1 infection. As a result, cART has prolonged the lifespan of people with HIV aiding these patients to age with the virus. Clinical evidence has established the long-term effects of cART in the aging population causing neuropsychiatric disorders such as depression. However, the underline molecular mechanism that cART induces depression-related neurodegeneration has not been established. This study will pursue a dopamine (DA) dysregulation marker in cART …

Understanding The Developmental Regulation Of Opa1 Processing In C2c12 Myoblasts, Harpreet Kaur

Theses and Dissertations

Mitochondria is an energy-producing organelle in the cell that accept electrons from the Krebs cycle to generate energy in the form of ATP by oxidative phosphorylation. Two mitochondrial aspects, bioenergetics and dynamics are critical for energy balance and cellular stress responses. Mitochondrial dynamics refers to the balance between mitochondrial fusion (mitochondrial biogenesis) and mitochondrial fission (mitochondrial fragmentation). Cellular stress conditions that lead to dissipation of transmembrane potential (Δψm) trigger the activation of an IMM protein, OMA1, a metalloprotease that leads to processing of another IMM protein, optic atrophy-1 (OPA1) that mediates IMM fusion. Persistent stress primes the mitochondria to mitophagy …

Influence Of Cover Crops On Soybean Yield And Partial Returns As An Alternative To Double-Crop Soybean In Arkansas, Drew Kirkpatrick, Trenton L. Roberts, Kris Brye, Jeremy Ross

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The number of hectares dedicated to winter wheat (Triticum aestivum L.) in the southern United States has been trending downward for almost a decade, presenting an opportunity for producers to utilize alternatives in their soybean [Glycine max (L.) Merr.] rotations, such as winter cover crops. A study was established to compare the cover crops to traditional cropping systems by determining the effect each had on a successive soybean crop and to examine partial economic returns. Cropping systems implemented were winter fallow, winter wheat, and seven cover crop treatments, including cereal rye (Secale cereale L.), black-seeded oat ( …

Dicamba Air Concentrations In Eastern Arkansas And Impact On Soybean, Maria Leticia Zaccaro-Gruener, Jason K. Norsworthy, Chad B. Brabham, L. Tom Barber, Trenton Roberts, Andronikos Mauromostakos, Thomas C. Mueller

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Damage to non–dicamba resistant (non-DR) soybean [Glycine max (L.) Merr.] has been frequent in geographies where dicamba-resistant (DR) soybean and cotton (Gossypium hirsutum L.) have been grown and sprayed with the herbicide in recent years. Off-target movement field trials were conducted in northwest Arkansas to determine the relationship between dicamba concentration in the air and the extent of symptomology on non-DR soybean. Additionally, the frequency and concentration of dicamba in air samples at two locations in eastern Arkansas and environmental conditions that impacted the detection of the herbicide in air samples were evaluated. Treatment applications included dicamba at …

Validity Of The Loadsol Pro Insole For Pedal Reaction Force Measurements During Stationary Cycling, Anabelle Vallecillo Bustos

Honors Theses

Advancements in wearable technology have allowed clinicians, coaches, and researchers the ability to observe and quantify human movement outside the laboratory. Instrumented insoles are an example of novel technology that can be worn in the shoes and measure vertical reaction force wirelessly. The use of such insoles will prove to be beneficial for athletes as they train, patients as they progress through rehabilitation, and researchers as they experiment in their respective fields. The Loadsol Pro (Novel Inc., St Paul., MN, USA) has been shown to produce accurate and reliable measures of ground reaction forces (GRF) in various dynamic activities including …

Uptake And Localization Of Poly(2-Vinyl-4,4-Dimethyl Azlactone) Modified With Rhodamine- And Coumarin-Based Molecules In Human Embryonic Kidney (Hek293) Cells, Garrett Tassin

Honors Theses

Fluorescence microscopy is a bioimaging technology that utilizes the excitation and emission of fluorophores to identify cellular structures, processes, and interactive events. Natural and synthetic organic dyes are frequently used in fluorescence microscopy techniques for imaging, therapeutic, and biomedical applications. It is also employed in the development of novel organic dyes for innovative methods of drug delivery and labelling. In this study, we investigate the behavior of an organic dye consisting of the post-polymerization modification of poly(2-vinyl-4,4-dimethyl azlactone) (PVDMA) with tetramethyl rhodamine cadaverine (TMR) and a coumarin-based molecule (DBAC). Using cultured HEK293 cells and fluorescence confocal microscopy, we show the …

Impact Of Training Volume On Female Athlete Triad Risk For Female Collegiate D1 Track And Field Athletes, Sarah Parnell

Honors Theses

iv ABSTRACT The Female Athlete Triad is the joint-presentation of three intertwined conditions (i.e., low energy availability (LEA), low bone mineral density (BMD), and menstrual dysfunction) that range in severity across affected individuals and have been known to affect the mental, emotional, and physical health of female athletes in a number of sports, especially those that are leanness-orientated and feature a high prevalence of disordered eating. The purpose of this study is to assess the potential relationship between increasing amounts of training volume and individual Triad risk factors in women’s collegiate track and field. Online Qualtrics surveys were administered to …

Morphological And Functional Classification Of The Amblyomma Americanum (Lone Star Tick) Hemocytes, Julia Hanson

Honors Theses

Ticks are a diverse group of obligate blood-feeding (hematophagous) arthropods that transmit a wide array of human and animal pathogens. These pathogens have caused an increase in tick-borne diseases that pose a significant threat to public health without preventative measures. These pathogens interact with the tick immune system upon uptake, which is primarily divided into humoral and cellular components. Cellular immunity in ticks encompasses several pathogen-eliminating mechanisms including phagocytosis. These mechanisms are mediated by the action of immune cells, commonly referred to as hemocytes, which are analogous to mammalian white blood cells. This study utilized a combination of morphological, functional, …

Developing A Continuous Cell Line Derived From The Gulf-Coast Tick (Amblyomma Maculatum) Embryos, Sabrina Walia

Honors Theses

Tick cell lines provide an affordable, reproducible, and efficient in vitro experimental system to study tick-pathogen interactions at the cellular level. Before the first tick cell lines were developed, there was no established method to perform in vitro studies with ticks. Tick research at the organismal level is full of difficulties and challenges. An increase in tick-borne infections and the availability of cutting-edge molecular tools have stimulated the research community's interest in using tick cell lines to answer important biological questions related to new and emerging tick-borne diseases. Unfortunately, not all medically relevant tick species have a cell line available. …

Assessment Of The Knowledge, Attitude, And Practice Towards Expired Drug Disposal Among The Community In Beirut City, Lebanon, Nada Khansa, Azza A. K. Gazy, Abdalla El-Lakany, Souraya Domiati

BAU Journal - Health and Wellbeing

The global increase in pharmaceutical spending has led to enhanced international awareness of the unused and expired drug issues due to improper drug disposal's harmful economic, environmental, and health effects. Consequently, the study was conducted to assess the knowledge, attitude, and practice toward expired drug disposal among the community in Beirut City, Lebanon. A cross-sectional, observational, questionnaire-based study was conducted. Data were analyzed using SPSS version 23. The fact that improper disposal of expired medicine affects the environment and health was acknowledged by 70.7% of the 450 participants. Even though 72.2% of the participants knew that the best method for …

Validation Of The Arabic Version Of The Food Safety And Hygiene Questionnaire Among Nutrition Student, Nada El Darra, Dina Cheaib, Leila Itani

BAU Journal - Health and Wellbeing

There is shortage of data on food safety knowledge and practices among Lebanese food handlers due to the lack of a reliable tool. The aim of the current study is to determine the reliability as well as the validity of an adopted Arabic version of the Osaili et al (2013) food safety questionnaire. Translation and back translation and testing equivalence were done using standard accepted procedures. A total of 110 undergraduate Nutrition and Dietetics students completed the questionnaire consisting of five subscales that covers major food safety concepts including personal hygiene, safe storage, cross contamination prevention, knowledge of health problems …