Life Sciences Commons^™

Early Solid Diet Supplementation Influences The Proteomics Of Rumen Epithelium In Goat Kids, Yimin Zhuang, Xiaokang Lv, Kai Cui, Jianmin Chai, Naifeng Zhang

Animal Science Faculty Publications and Presentations

It is well known that solid diet supplementation in early life can significantly promote rumen development and metabolic function in young ruminants. However, the changes in the expressed proteome and related metabolism in rumen epithelium in response to a supplemented solid diet remain unclear. In this study, rumen epithelial tissue from goats in three diet regimes including milk replacer only (MRO), milk replacer supplemented concentrate (MRC), and milk replacer supplemented concentrate plus alfalfa pellets (MCA) were collected for measurement of the expression of epithelial proteins using proteomic technology (six per group). The results showed that solid diet significantly improved the …

Developing A Continuous Cell Line Derived From The Gulf-Coast Tick (Amblyomma Maculatum) Embryos, Sabrina Walia

Honors Theses

Tick cell lines provide an affordable, reproducible, and efficient in vitro experimental system to study tick-pathogen interactions at the cellular level. Before the first tick cell lines were developed, there was no established method to perform in vitro studies with ticks. Tick research at the organismal level is full of difficulties and challenges. An increase in tick-borne infections and the availability of cutting-edge molecular tools have stimulated the research community's interest in using tick cell lines to answer important biological questions related to new and emerging tick-borne diseases. Unfortunately, not all medically relevant tick species have a cell line available. …

Impact Of Lyophilization On Porcine Hemoglobin Properties., Mustafa Almosawi

College of Arts & Sciences Senior Theses

Blood transfusion is the single most often performed lifesaving procedure in hospitals worldwide. Unfortunately, packed red blood cells (RBCs) used for transfusion can only be stored for 42 days at 4 °C before being discarded due to irreversible damage that occurs during storage. Any reduction in available RBCs for an extended period can lead to blood shortages. To increase the shelf-life of RBCs, we investigated freeze-drying (lyophilizing) in the presence of the non-toxic sugar trehalose as a method for long-term preservation. However, the oxidative stress of the lyophilization and storage processes can compromise the functionality of these cells, and the …

Seasonal Variation In Condition, Body Lipid, Reproductive Investment, And Diet In Brook Stickleback (Culaea Inconstans), Matthew P. Basista, Randal Snyder, Christopher Pennuto, Alicia Perez-Fuentetaja

Great Lakes Center Masters Theses

North-temperate fishes are subject to significant changes in abiotic and biotic conditions across seasons, which are likely reflected in temporal differences in energy dynamics, reproductive investment, and diet. This study explores seasonal changes in body lipid content, female reproductive investment (GSI), body condition (Fulton’s K), and diet in brook sticklebacks (Culaea inconstans) in Western New York. I expected body lipid content and body condition would decline during the breeding season reflecting energy expenditure for reproduction, and these parameters would increase in the summer and fall prior to the onset of winter. Based on previous studies, I also expected …

A Field‐Capable Rapid Plant Dna Extraction Protocol Using Microneedle Patches For Botanical Surveying And Monitoring, Jonathan Selz, Nicolas R. Adam, Céline E. M. Magrini, Fulvia Malvido Montandon, Sven Buerki, Sebastian J. Maerkl

Biology Faculty Publications and Presentations

Premise: A novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is validated by sequencing and comparing the results with QIAGEN spin-column DNA extractions using BLAST analyses.

Methods and Results: Two sets of DNA extractions were conducted on 13 species spanning various leaf anatomies and phylogenetic lineages: (i) fresh leaves were punched with custom polymeric microneedle patches to recover genomic DNA, or (ii) QIAGEN DNA extractions. Three plastid (matK, rbcL, and trnH-psbA …

Psychopathy In Society: Understanding The Neural Mechanisms That Give Rise To Psychopathic Traits, Phoebe Zalenski

Psychological Science Undergraduate Honors Theses

Psychopathy is characterized as an individual exhibiting callousness, grandiosity, lack of empathy, and manipulative behavior towards others over a long period of time. Given these symptoms, psychopathy is associated with a markedly increased risk of arrest and imprisonment. Together, these findings highlight the importance of understanding the cause(s) of psychopathy, as doing so may help to develop treatments or preventative interventions. Prior work has suggested that structural abnormalities in the amygdala may play a role in clinical psychopathy; however, it is less clear whether amygdala abnormalities exist on a continuum of psychopathic traits. In this study, I aimed to determine …

Effects Of Cyclic Heat Stress On The Acute Inflammatory Response In Broilers, Alessandro J. Rocchi

Poultry Science Undergraduate Honors Theses

Heat stress (HS) is a growing concern in broiler production due to increasing environmental temperatures. Little is known of the overall effect of HS on innate immunity. Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, is commonly used to study the inflammatory response. In avian species, the local tissue and systemic inflammatory activities in response to LPS may be determined concurrently, over time, in an individual, using the growing feather (GF) pulp dermal test along with blood measurements (dual-window approach). To examine the effect of cyclic HS on the local and systemic acute inflammatory responses, Cobb 500 male broiler …

A Dna-Peptide Crosslink (Dpc) Increases Mutagenicity In Sos-Induced Escherichia Coli, Alessandra Bassani

Honors Scholar Theses

Bacteria, such as Escherichia coli, have an inducible system in response to DNA damage termed the SOS response. This system is activated when the replicative DNA polymerase (Pol) III encounters a lesion, uncouples from DNA helicase, and single-stranded DNA (ssDNA) accumulates at the replication fork. In this study, we investigated DNA-peptide crosslink (DpC), a common lesion that results from cross-linking of proteins or peptides, UV irradiation, and alkylating agents. To increase survival following formation of a lesion, the SOS response can utilize homologous recombination, translesion synthesis (TLS), or excision repair. With TLS, the levels of DNA Pol II, IV, …

Use Of Space And Foraging Behavior Of Loggerhead Shrikes In Urban Areas, Katie Anne Maddox

Electronic Theses and Dissertations

Loggerhead shrikes, Lanius ludovicianus, are a declining grassland songbird native to North America. Land-use change has been associated with the decline of grassland bird species. However, the process of urbanization creates novel short-grass habitats reminiscent of shrikes’ natural habitats. Loggerhead shrikes have been observed living in developed areas, particularly in Southeastern coastal states. Understanding the long-term impacts of urban-dwelling on loggerhead shrike populations is vital to identifying whether urban areas offer refuge or act as an ecological sink for shrike populations.

In chapter one, I quantified the use of space by shrikes in urban habitats within Horry County, South …

Deterrent Effects Of The Select Magnetic And Repellent Treated (Smarttm) Hooks And The Recently Developed "Smarter" Hooks On Sharks In Recreational Hook-And-Line And Longline Trials, Ryan Lowndes

Electronic Theses and Dissertations

As bycatch continues to impact global shark populations, there is a continuing need for effective bycatch reduction devices. Prior research has shown promise in exploiting sharks' electrosensory ability to this end. We tested the deterrent efficacy of the Select Magnetic and Repellant Treated (SMARTTM) and the newly developed "SMARTER" hooks in experimental longline and hook-and-line trials. Both are magnetized and contain an electropositive metal component made of magnesium (SMART) and a magnesium alloy designed to extend longevity (SMARTER). We deployed 127 longlines with SMART hooks, SMARTER hooks, controls, and procedural controls from 2021-2022 in Winyah Bay, South Carolina, and caught …

Exploring The Role Of Microrna-1 (Mir-1) On Skeletal Muscle Hypertrophy, Shengyi Fei

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Skeletal muscle hypertrophy is a complex process that involves a range of signaling pathways and transcriptional regulators. Many hormones and growth factors can activate key signaling pathways, such as the PI3K/AKT/mTOR, MAPK, and cAMP pathways, which play a crucial role in the regulation of muscle hypertrophy. In Chapter 1, we reviewed some of the hormones and growth factors known to be associated with skeletal muscle hypertrophy, as well as the function of these key signaling pathways, and revealed some unresolved issues. In Chapter 2, we explored the role of microRNA-1 (miR-1) in skeletal muscle hypertrophy and aimed to determine the …

Gene Expression Changes Linked To Phenylpropanoid-Based Resistance To Fusarium Head Blight Of Wheat, Shiv Singla

Department of Plant Pathology: Dissertations, Theses, and Student Research

Fusarium graminearum is a devastating pathogen of wheat that causes Fusarium head blight (FHB) and contaminates the grain with the mycotoxin deoxynivalenol (DON). Resistance to FHB is quantitative and it is important to identify additional genes conferring resistance against it. The goal of this thesis was to examine if the constitutive expression of two sorghum phenylpropanoid pathway genes, SbCCoAOMT (encoding caffeoyl-CoA O-methyltransferase) and SbC3’H (encoding p-coumarate 3-hydroxylase), in the moderately-susceptible spring wheat CB037 can provide Type-I and Type-II resistance to F. graminearum and determine the underlying mechanisms of the enhanced resistance. The constitutive expression lines (CCoAOMT413, CCoAOMT421, C3H112, …

Optimizing Soil Nutrient Management To Improve Dry Edible Bean Yield And Protein Quality, Emily Jundt

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Soil nutrient management is of the utmost importance to ensure crop yields are maximized to feed the world’s growing population. Micro and macronutrient deficiencies can be detrimental to plant growth and cause a drastic reduction in yield. Optimization of nutrient management needs extensive research. Nutrients such as nitrogen increase crop yield, even in legumes capable of using a large amount of nitrogen (N) via biological N fixation. Iron (Fe) fertilizers are also crucial for legumes as they are susceptible to Fe deficiency induced chlorosis.

Dry edible beans (Phaseolus vulgaris) are leguminous plants and excellent dietary protein sources. Great …

Importance Of Pelvic Floor Education And Rehabilitation Techniques For Adolescents And Young Adults: A Review, Katherine Laura Schmidt

Honors Program: Senior Projects (Public)

The pelvic floor is the collection of many muscles forming what is often called the pelvic girdle. Understanding the different muscles and their roles is an important part of pelvic floor education. Although both males and females have pelvic floors, there is a lack of knowledge of basic anatomy seen in adolescents and young adults. This review analyzes the importance of providing more education for students about the pelvic floor, gives examples of common pelvic disorders for young adults, and provides details on the physical therapy rehabilitation techniques offered today to treat these conditions.

Extranodal Tumors And Chromosomal Abnormalities Associated With Non-Hodgkin Lymphoma In T(14;18)-Positive Patients, Alexis Finch

Honors Program: Senior Projects (Public)

Nebraska is mostly an agricultural state with a non-Hodgkin lymphoma (NHL) incidence rate of 20.1 per 100,000 individuals, which is greater than the US incidence rate of 19 per 100,000 individuals. It is possible that the higher incidence rate in Nebraska is due to an increase in pesticide usage. Pesticide exposure is linked to t(14;18), a genetic translocation involving the BCL2 gene that regulates apoptosis. The dysregulation of apoptosis could contribute to the proliferation of NHL. This exploratory study utilized the Nebraska Lymphoma Study Group, which consists of patients with non-Hodgkin lymphoma, Hodgkin disease, or some other neoplastic hematologic disorder …

Antimicrobial Resistance In Eskape Pathogens And Its Effect On Modern Medicine And Treatment, Cameran Runge

Honors Program: Senior Projects (Public)

Abstract

ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella

pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter

spp.) are seeing a growing resistance to multiple classes of antibiotics. Misuse and overuse of antibiotics have played directly into the resistance observed, and the problem is growing exponentially. Antibiotic resistance is partially due to several intrinsic factors limiting the drug's uptake. These include efflux pumps, increased biofilm production, and reduced cell wall permeability in the resistant bacteria. ESKAPE pathogens also acquire resistance through horizontal gene transfer and plasmids. As antibiotics have become less effective, the bacteria can continue to thrive, leading to a detrimental …

Education, Beliefs, And Health Literacy Of Care Providers On Mouth Care Of Older Adults, Molly Lakin

Honors Program: Senior Projects (Public)

Oral health is important to one’s overall health and quality of life as it can lead to chronic illnesses such as cardiovascular disease, stroke, and neurodegenerative disorders (Haumschild et al. 2021). In long-term care facilities, it is important that dependent older adults are provided mouth care to maintain their oral health and decrease their chances of developing a chronic illness; however, there are barriers affecting the level, quality, and quantity of mouth care being provided in these facilities. A survey was developed to look at the education, beliefs, and health literacy of care providers on providing mouth care in hopes …

A Review Of How Bioinformatics And Genome Sequencing Are Affecting Precision Medicine, Taylor S. Hickey

Honors Program: Senior Projects (Public)

Advancement in genomic sequencing and bioinformatics methods have been affecting biomedical research through precision medicine, especially in the area of cancer. Vaccine therapies can be developed using neoantigens that target specific mutations in tumors. The goals of this research are to identify mutations that lead to cancer and then define subpopulations in which patients can easily be identified. The future goal is to have targeted vaccines that are specific to each subpopulation ready to be used in treatment of their cancer. Limitations to reaching these goals have been due to tumor heterogeneity, cancer location, and difficulty in creating neoantigens for …

Evaluation Of Anaplasma Marginale Elisa Positive Cattle For Co-Infection With Ehrlichia Spp., Grayson Robbins

Animal Science Undergraduate Honors Theses

Bovine Anaplasmosis is an infectious, hemolytic disease transmitted by the rickettsia parasite Anaplasma marginale in cattle. A. marginale parasitizes the red blood cells of an animal throughout their lifetime. The beef cattle industry is the fifth largest agricultural commodity in Arkansas, so potential economic losses due to bovine anaplasmosis is a critical issue. Previously tested beef cattle herds at the University of Arkansas’ Savoy and Batesville units have tested seropositive for A. marginale with low percent inhibitions; however, all seropositive animals tested negative for infection on DNA analysis. The objective of this study was to determine the current infection status …

Diversification And Convergence Following The Transition From Saltwater To Freshwater In Stingrays., Autumn D. Magnuson

College of Arts & Sciences Senior Theses

One of the most fundamental questions in biology is why some groups of organisms are more diverse than others. Classic hypotheses for explaining differences in diversity consider distinctions in time, place, resources, and competitors as the staging grounds for differential diversification. Freshwater and saltwater environments have similar levels of diversity despite significant differences in size, so studying transitions between the two systems can provide insights into evolutionary processes. Despite the challenges associated with this transition, stingrays have invaded freshwater habitats multiple times across different continents, making them useful for better understanding these systems. In this study, I evaluated the frequency …

Osteoimmunology And The Reconstruction Of Host Immunological Status In Treponemal Infection: Effect Of Activated Immune Cells By Oral Pathogens And Treponema Pallidum On Osteoclastogenesis., Michayla Gatsos

College of Arts & Sciences Senior Theses

Syphilitic infection caused by bacterium Treponema pallidum pallidum (Tp) offers an excellent model to study the long-lasting interplay between the immune and skeletal systems and could be used to reconstruct host immunological status. We propose that a hyper-inflammatory phenotype developed during acquired syphilis will have a systemic impact on most bone microenvironments involving inflammatory processes, such as the one developed in periodontal disease (PD). Using osteoimmunological in vitro protocols, we explore whether immune activation by Tp antigens and PD pathogens can impact osteoclastogenesis (OCG), ultimately helping to understand the bone alterations caused by systemic inflammatory processes in skeletal material. We …

Obstacle Crossing In Healthy Young And Older Individuals, Hope M. Hanson, Ashlyn M. Jendro, Abigail C. Schmitt

Health, Human Performance and Recreation Undergraduate Honors Theses

Introduction: In the United States, the average population age is rising and will continue to increase in the coming years.With an older population comes increased risk of injury associated with falls. Falls are considered a leading cause of injury and death in older individuals, and many falls are caused by body imbalance or obstacle collision due to a clearly visible stationary object (e.g., rug, chair, branch). Older adults tend to cross obstacles with increased toe clearance in order to prevent tripping, but much of what is known about obstacle crossing in older adults is limited to artificial obstacles that are …

Engaging Older Adults In Local Nonprofit Nutrition Assistance Programs: Understanding Barriers And Exploring Opportunities For Improved Participation, Amber R. Godinsky

Nonprofit and NGO Studies Student Projects

The rapidly growing population of older adults in the United States is set to require a highly skilled and specialized healthcare response to control the threat of a public health crisis. Research on approaches to mitigation suggests the need for an emphasis on preventative measures such as health promotion and nutrition intervention to be used as vital tools in local communities. This case study on older adults residing within DeKalb County, Illinois offers insight into the accessibility of nonprofit nutritional assistance programs by providing a local snapshot of the barriers to participation in a preventative health resource. Methods of collecting …

Development Of A Computational Model To Investigate Pathways And The Effects Of Treatment In Fanconi Anemia, Sabrina Kellett

Biological Sciences Undergraduate Honors Theses

Fanconi Anemia (FA) is a rare type of anemia that is not easily studied and can have very detrimental effects. This disease compromises the bone marrow, resulting in decreased hemopoiesis. Symptoms of FA also include abnormalities in the brain and spinal cord, incorrect formation of the kidneys, abnormal formation of the heart and lungs, and a dramatically increased risk of developing cancer. FA can be caused by various mutations in any of the 22 genes that encode for proteins involved in what is called the FA DNA repair pathway. In healthy individuals, this pathway specifically repairs interstrand cross-links (ICLs) recognized …

Sequence Analysis Of Herbicide Target Genes In Herbicide-Tolerant Rice, Jonathan Kearney, Nilda R. Burgos, Gulab D. Rangani

Biological Sciences Undergraduate Honors Theses

The tolerance of mutagenized rice (Oryza sativa) lines to selector herbicides was investigated and the sequence of ALS gene was analyzed in plants that survived treatment with 4x the label rate of imazethapyr herbicide. This was done to determine if insensitivity to imazethapyr is due to mutation(s) in the herbicide binding site. Seedlots previously treated with ethyl-methyl sulfonate were planted in the field and 3-leaf seedlings were treated with various herbicides to screen for herbicide-tolerant mutants. Seeds from survivors composed the rice lines tested in the current research. Seeds were planted in the greenhouse and 3-leaf seedlings were …

School Structure Differences And Physical Activity Of Youth: A Case Study Of Four Rural Communities, Jillian L. Kilty

Health and Kinesiology Theses, Dissertations, and Student Creative Activity

Purpose: To describe youth physical activity (PA) and participation in organized activities within school systems adopting different organizational structures. Design: Cross-sectional case-study of the Wellscapes Project with baseline data from Wave 1 (Fall 2018) and Wave 2 (Fall 2021). Setting: Four rural Midwestern communities. Sample: A total of 507 fourth, fifth, and sixth graders participated and were used in analyses (n = 156 fourth graders; n =189 fifth graders; n = 162 sixth graders). Two communities followed an elementary school model. The other two communities followed a middle school model. Measures: Students completed the Youth Activity Profile (YAP), an online …

Interactive Map Code (R And Rstudio), Peter G. Butterfield

Data Files: White Oaks in Vinton Furnace Experimental Forest

Interactive map code puts data and coordinates into readable table format and creates data visualizations.

Microorganisms In Extreme Environmental Conditions, Khanh Mai Nguyen

Graduate Theses and Dissertations

Organisms are known to be able to prosper under normal and extreme environmental conditions, which are classified as mesophile and extremophile, respectively. Extremophiles can thrive under a large array of conditions, from pressures, temperatures, salinity, and pH to a combination of them. For example, to survive on the ocean floor, marine biomass must have its biomolecular machinery adapted to the high pressures and high salinity environment. Moreover, around the hydrothermal vents, aside from pressure and salinity, the microbes that live there also need to adjust to the temperature as well as the pH level. Aside from high temperatures, researchers also …

Investigation The Plugging Behavior Of Virus Filters, Iman Abdulqader Saab

Graduate Theses and Dissertations

A virus filtration step is integral to the manufacturing of biopharmaceuticals to ensure viral safety. A virus filter is a single-use device that uses a size-based separation process and has a unique structure with minimal defects, so that contaminating virus particles cannot pass through the membrane pores, while therapeutic molecules can. The development of novel antibodies (Abs), including significant increases in product titers, is frequently challenged by virus filter fouling, making a better understanding of the underlying mechanisms essential. This thesis focused investigating of the effect of prefilter types, buffer type and salt content on virus filtration performance. The impact …

Unveiling The Ancestral Function Of A Neuroendocrine Regulator, Pou-I/Pit1: Insights From Gene Expression Analysis In The Sea Anemone Nematostella Vectensis, Miguel Agostinho Pina Da Silva

Graduate Theses and Dissertations

Cnidaria (i.e., sea anemones, jellyfish, corals) and Bilateria (i.e., vertebrates, sea stars, fruit flies), are sister groups that diverged around 600 million years ago. Despite the long evolutionary time, many cellular differentiation mechanisms, cell types, tissues and behaviors are conserved. Such as neurons, mechanosensory hair cells, feeding behaviors, peristaltic movements, and sleep. Recent advances in genomics, molecular biology and microscopy have fueled an increased interest in understanding cnidarian nervous and neuroendocrine systems. Understanding the developmental mechanisms and the mode of operation of Cnidarian nervous systems helps to reconstruct the ancestral nervous system of the last common ancestor of Cnidaria and …