Life Sciences Commons^™

Is Vdac1 A Novel Bcl2 Family Member That Binds Bax?, Claire Pearson

Honors Theses

Apoptosis is a type of regulated cell death important for normal embryonic development and maintenance of adult tissues by removing excess or dysfunctional cells to ensure proper functioning of organs. The Bcl-2 family of proteins determines whether apoptosis remains suppressed or becomes activated through the balance of interactions among pro-survival and pro-death members. A defining feature of the Bcl-2 family is a BH3 domain that drives interactions between the family members. Isoform 1 of the voltage dependent anion channel (VDAC1) has an important role in metabolism, but was recently found to have high homology with known BH3 domains. This study …

Parkin Ubiquitination Of Kindlin-2 Enables Mitochondria-Associated Metastasis Suppression, Minjeong Yeon, Irene Bertolini, Ekta Agarwal, Jagadish C Ghosh, Hsin-Yao Tang, David W. Speicher, Frederick Keeney, Khalid Sossey-Alaoui, Elzbieta Pluskota, Katarzyna Bialkowska, Edward F. Plow, Lucia R. Languino, Emmanuel Skordalakes, M Cecilia Caino, Dario C. Altieri

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Mitochondria are signaling organelles implicated in cancer, but the mechanisms are elusive. Here, we show that Parkin, an E3 ubiquitination (Ub) ligase altered in Parkinson's disease, forms a complex with the regulator of cell motility, Kindlin-2 (K2), at mitochondria of tumor cells. In turn, Parkin ubiquitinates Lys581 and Lys582 using Lys48 linkages, resulting in proteasomal degradation of K2 and shortened half-life from ∼5 h to ∼1.5 h. Loss of K2 inhibits focal adhesion turnover and β1 integrin activation, impairs membrane lamellipodia size and frequency, and inhibits mitochondrial dynamics, altogether suppressing tumor cell-extracellular matrix interactions, migration, and invasion. Conversely, Parkin does …

Transcriptome Profile In Drosophila Kc And S2 Embryonic Cell Lines, Daniel Klonaros, Jacqueline M. Dresch, Robert A. Drewell

Biology

Drosophila melanogaster cell lines are an important resource for a range of studies spanning genomics, molecular genetics, and cell biology. Amongst these valuable lines are Kc167 (Kc) and Schneider 2 (S2) cells, which were originally isolated in the late 1960s from embryonic sources and have been used extensively to investigate a broad spectrum of biological activities including cell-cell signaling and immune system function. Whole-genome tiling microarray analysis of total RNA from these two cell types was performed as part of the modENCODE project over a decade ago and revealed that they share a number of gene expression features. Here, we …

Does Vdac2 Have A Bh3 Domain?, Lillian Ferkany

Honors Theses

Mitochondrial outer membrane permeabilization (MOMP) by Bax oligomerization triggers apoptosis. BCl-2 family proteins, classified as BH3 only proteins, pro-survival proteins, or pro-apoptotic proteins, control apoptosis partly through their agonist or antagonistic effects on Bax, which are mediated by their conserved BH3 domains. All BH3 domains form an alpha helix containing 5-7 conserved hydrophobic residues, designated H0-H5, and one conserved aspartic acid that drive interaction with Bax and other ‘multi-domain’ BCl-2 members. BH3 agonists induce Bax oligomerization, while BH3 antagonists sequester Bax to prevent MOMP. We discovered that voltage dependent anion channels (VDACs) in the MOM contain a putative BH3-like domain …

Association Of Mitochondrial Dna Copy Number With Brain Mri Markers And Cognitive Function: A Meta-Analysis Of Community-Based Cohorts, Yuankai Zhang, Xue Liu, Kerri L Wiggins, Nuzulul Kurniansyah, Xiuqing Guo, Amanda L Rodrigue, Wei Zhao, Lisa R Yanek, Scott M Ratliff, Achilleas Pitsillides, Juan Sebastian Aguirre Patiño, Tamar Sofer, Dan E Arking, Thomas R Austin, Alexa S Beiser, John Blangero, Eric Boerwinkle, Jan Bressler, Joanne E Curran, Lifang Hou, Timothy M Hughes, Sharon L R Kardia, Lenore J Launer, Daniel Levy, Thomas H Mosley, Ilya M Nasrallah, Stephen S Rich, Jerome I Rotter, Sudha Seshadri, Wassim Tarraf, Kevin A González, Vasan Ramachandran, Kristine Yaffe, Paul A Nyquist, Bruce M Psaty, Charles S Decarli, Jennifer A Smith, David C Glahn, Hector M González, Joshua C Bis, Myriam Fornage, Susan R Heckbert, Annette L Fitzpatrick, Chunyu Liu, Claudia L Satizabal

Faculty, Staff and Student Publications

BACKGROUND AND OBJECTIVES: Previous studies suggest that lower mitochondrial DNA (mtDNA) copy number (CN) is associated with neurodegenerative diseases. However, whether mtDNA CN in whole blood is related to endophenotypes of Alzheimer disease (AD) and AD-related dementia (AD/ADRD) needs further investigation. We assessed the association of mtDNA CN with cognitive function and MRI measures in community-based samples of middle-aged to older adults.

METHODS: We included dementia-free participants from 9 diverse community-based cohorts with whole-genome sequencing in the Trans-Omics for Precision Medicine (TOPMed) program. Circulating mtDNA CN was estimated as twice the ratio of the average coverage of mtDNA to nuclear …

Microbial Biofortification: A Sustainable Solution To Feed 10 Billion People, Perrin Thompson, Kathryn Tschida

Celebrating Scholarship and Creativity Day (2018-)

No abstract provided.

Results Of Salvage Therapy With Mini-Hyper-Cvd And Inotuzumab Ozogamicin With Or Without Blinatumomab In Pre-B Acute Lymphoblastic Leukemia, Hagop Kantarjian, Fadi G Haddad, Nitin Jain, Koji Sasaki, Nicholas J Short, Sanam Loghavi, Rashmi Kanagal-Shamanna, Jeffrey Jorgensen, Issa Khouri, Partow Kebriaei, Yesid Alvarado, Tapan Kadia, Shilpa Paul, Guillermo Garcia-Manero, Bouthaina Dabaja, Musa Yilmaz, Jovitta Jacob, Rebecca Garris, Susan O'Brien, Farhad Ravandi, Elias Jabbour

Faculty, Staff and Student Publications

Background: Historically, adults with relapsed-refractory acute lymphoblastic leukemia (ALL) experienced poor outcomes with intensive chemotherapy. This mature analysis explores the benefit of the addition of sequential blinatumomab to low-intensity mini-Hyper-CVD chemotherapy with inotuzumab ozogamicin in this setting.

Methods: Mini-Hyper-CVD (cyclophosphamide and dexamethasone at 50% dose reduction, no anthracycline, methotrexate at 75% dose reduction, cytarabine at 83% dose reduction) was combined with inotuzumab during the first 4 courses. From Patient #68 and onwards, inotuzumab was given in reduced and fractionated doses, and blinatumomab was added sequentially for 4 courses. Maintenance therapy with prednisone, vincristine, 6-mercaptopurine and methotrexate was given for 12 …

Spatial Transcriptomics Depict Ligand-Receptor Cross-Talk Heterogeneity At The Tumor-Stroma Interface In Long-Term Ovarian Cancer Survivors, Sammy Ferri-Borgogno, Ying Zhu, Jianting Sheng, Jared K Burks, Javier A Gomez, Kwong Kwok Wong, Stephen T C Wong, Samuel C Mok

Faculty, Staff and Student Publications

Advanced high-grade serous ovarian cancer (HGSC) is an aggressive disease that accounts for 70% of all ovarian cancer deaths. Nevertheless, 15% of patients diagnosed with advanced HGSC survive more than 10 years. The elucidation of predictive markers of these long-term survivors (LTS) could help identify therapeutic targets for the disease, and thus improve patient survival rates. To investigate the stromal heterogeneity of the tumor microenvironment (TME) in ovarian cancer, we used spatial transcriptomics to generate spatially resolved transcript profiles in treatment-naïve advanced HGSC from LTS and short-term survivors (STS) and determined the association between cancer-associated fibroblasts (CAF) heterogeneity and survival …

A Geospatial Approach To Identify Patterns Of Antibiotic Susceptibility At A Neighborhood Level In Wisconsin, United States, Laurel Legenza, Kyle Mcnair, Song Gao, James P Lacy, Brooke J Olson, Thomas R Fritsche, Lucas T Schulz, Samantha Lamuro, Frances Spray-Larson, Tahmeena Siddiqui, Warren E Rose

Faculty, Staff and Student Publications

The global threat of antimicrobial resistance (AMR) varies regionally. This study explores whether geospatial analysis and data visualization methods detect both clinically and statistically significant variations in antibiotic susceptibility rates at a neighborhood level. This observational multicenter geospatial study collected 10 years of patient-level antibiotic susceptibility data and patient addresses from three regionally distinct Wisconsin health systems (UW Health, Fort HealthCare, Marshfield Clinic Health System [MCHS]). We included the initial Escherichia coli isolate per patient per year per sample source with a patient address in Wisconsin (N = 100,176). Isolates from U.S. Census Block Groups with less than 30 isolates …

Grafting Jig For Small-Diameter American Beech: Proof Of Concept And First Print File, Andrea L. Myers, Tara Bal

Michigan Tech 3D Designs

This tool may be useful for grafting other small diameter woody plant species. This jig can be used by non-experts to simplify and standardize the initial cuts necessary for side grafting small-diameter American beech. The initial file which is suitable for 3D printing is available here, whereas we have the intention of undergoing iterative revisions to refine the tool, considering different sizes and modifications. Future versions will be added through the same avenue as advancements are made.

Pno-Monia, Ayati Lala, Nandana Varma

Human Diseases Graphic Novels

No abstract provided.

Small Cell Lung Cancer, Ramzi Daki '23, Breena Bulthuis, Zander Davidson, Dammy Tabiti, Addie Kobayashi

Human Diseases Graphic Novels

No abstract provided.

Dynamic Transcriptomic Responses Of Circulating Immune Cells In Response To Subsequent Days Of Exercise Heat Stress, Soohyun Oh

University Scholar Projects

Acute exercise and chronic training have diverse effects on immune function that are still not well understood. There are remaining questions about how an acute bout of exercise, competition, or repeated training over a period affects an individual’s immune defense system. In a related study, there is evidence that acute exercise may serve as an adjuvant to enhance response to immune function as well as contradicting evidence, including our preliminary data (unpublished). Understanding the fundamental function and gene expression of circulating immune cells in response to acute exercise and in various contexts such as heat stress is critical in understanding …

An Analysis Of Sediment Collected By Pogonomyrmex Salinus From The Jurassic Sundance Formation, Bighorn Basin, Wyoming, Josephine H. Laprad

Graduate Dissertations and Theses

Pogonomyrmex salinus is a species of harvester ant found in the Bighorn Basin area of Wyoming. Harvester ants are aptly named as they “harvest” seeds for food, but this harvesting behavior is also seen in their collection of sediment particles to build their mounds. For decades, paleontologists have looked to harvester ants for microfossils because the small size of the fossils makes them difficult for human eyes to find. But there are unresolved questions about the data that can be gleaned from the fossils collected by the ants. The mounds collected for this study include material from the Stockade Beaver …

The Effect Of Joint Versus Soft Tissue Flossing On Overall Performance In The Lower Extremities, Emily Bray

Graduate Dissertations and Theses

Effective warm-up routines result in a reduction in muscle soreness post-exercise, a lessened risk of injury, and an enhanced performance output ability. Existing research has shown promising effects of muscle tissue flossing, a novel method of warming up in which a latex band is used to apply pressure to a specific area in the body. The purpose of this study was to determine how active college students respond to flossing applied to both a joint as well as soft tissue in the lower extremities, focusing on the ankle joint and calf muscle. Floss bands were applied bilaterally for each of …

Self-Selected Pacing Strategy And Lactate Response In Individual Medley Time Trials, Claire Galloway

Graduate Dissertations and Theses

The 200 and 400-yard individual medley (IM; 200 IM and 400 IM, respectively) are two swimming events that challenge swimmers' versatility. Each of these events has different physiological requirements, where the 200 IM is viewed as an anaerobic event and the 400 IM as an aerobic event. Additionally, the IMs require performance in all four competitive stroke disciplines in a specific order (butterfly, backstroke, breaststroke, and freestyle), with each stroke requiring a different magnitude of intensity, efficiency, and speed. Purpose: To study the different pacing strategies and corresponding blood lactate response during the 200 IM and 400 IM in National …

An Analysis Of Sediment Collected By Pogonomyrmex Salinus From The Jurassic Sundance Formation, Bighorn Basin, Wyoming, Josephine H. Laprad

Graduate Dissertations and Theses

Pogonomyrmex salinus is a species of harvester ant found in the Bighorn Basin area of Wyoming. Harvester ants are aptly named as they “harvest” seeds for food, but this harvesting behavior is also seen in their collection of sediment particles to build their mounds. For decades, paleontologists have looked to harvester ants for microfossils because the small size of the fossils makes them difficult for human eyes to find. But there are unresolved questions about the data that can be gleaned from the fossils collected by the ants. The mounds collected for this study include material from the Stockade Beaver …

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2021, Leah English, Jennie Popp

AAES Research Reports and Research Bulletins

Agriculture and associated agricultural activities are major contributors to the Arkansas economy. Agriculture is defined as the sum of agricultural production and processing activities, unless otherwise specified, and includes crop and animal production and processing, agricultural support industries, forestry and forest products, and textile goods. Agriculture contributes to the economy through direct agricultural production and processing, leading to economic activity in other parts of the economy.

This report is the sixteenth in a series that examines agriculture’s economic contribution to the Arkansas economy. The total economic contribution of agriculture (direct, indirect, and induced effects) on value added, employment, and labor …

Effect Of Temperature On The Microbiome Of A Laboratory Reared Colony Of Haemaphysalis Longicornis Ticks, Brianna Mitchell

Poster Presentations

Honors research poster.

Haemaphysalis longicornis is a species of tick native to eastern Asia, including eastern China, Japan, eastern Russia, and Korea. To date, it has invaded and has now established its existence in Australia, New Zealand, and several of the Pacific Islands. This species of tick has recently been introduced to the United States, having a confirmed sighting in November 2017 on a sheep farm in New Jersey and since establishing populations in 18 states along the east coast and Appalachia. Based on the existing locations of H. longicornis in its native regions, as well as in the United …

The Role Of Fto, A Human Rna Demethylase In Perennial Grass Development And Abiotic Stress Responses, Andrew Fiorentino, Qian Hu, Xiaotong Chen, Zhaohui Chen

Honors College Theses

The integration of the human fat mass and obesity associated (FTO) gene into turfgrass is a novel approach at improving cell proliferation and abiotic stress resistance. The FTO protein is an RNA demethylase responsible for epigenetic regulation of the genome. In related rice, the gene is associated with increased crop yield, tiller number, and aerial biomass. It is proposed to work via demethylation of repeat RNA associated with chromatin remodeling, causing widespread transcriptional activation. In this study, the feasibility of using FTO for plant trait modification in perennial grasses is being investigated. Potentially transformed embryogenic calli of creeping bentgrass with …

Are Brain Games Really As They Seem?, Ania Axas

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Smart-Insect Monitoring System Integration And Interaction Via Ai Cloud Deployment And Gpt, Ahmed Moustafa

Computer Science and Computer Engineering Undergraduate Honors Theses

The Insect Detection Server was developed to explore the deployment and integration of an Artificial Intelligence model for Computer Vision in the context of insect detection. The model was developed to accurately identify insects from images taken by camera systems installed on farms. The goal is to integrate the model into an easily accessible, cloud-based application that allows farmers to analyze automatically uploaded images containing groups of insects found on their farms. The application returns the bounding boxes and the detected classes of insects whenever an image is captured on-site, enabling farmers to take appropriate actions to address the issue …

Temporal, Phenotypic, And Quantitative Characterization Of Thyroid Infiltrating Mononuclear Cells During Development Of Spontaneous Autoimmune Thyroiditis In Obese Strain Chickens, Katelyn M. Clark

Poultry Science Undergraduate Honors Theses

The Obese strain (OS) of chickens spontaneously develops autoimmune thyroiditis and is a well-established biomedical model for Hashimoto’s thyroiditis in humans. Both conditions are characterized by the infiltration of the thyroid glands with mononuclear cells resulting in destruction of the thyroid tissue and impairment of the thyroid’s endocrinological functions. Past studies have described mononuclear cell infiltration in thyroids of the OS chickens, but the time-course, cell composition, and relative amounts of the various mononuclear cells infiltrating the thyroids has not been well defined. In this project, the mononuclear cell infiltrate was characterized phenotypically and quantitatively in OS chickens over the …

Someday I Will Change The World: Creating Youth Change Agents Through Leadership Training, Colin Keady

Agricultural Education, Communications and Technology Undergraduate Honors Theses

No research currently exists on a leadership development program for secondary students that features project-based learning and connection with change agents. This project seeks to create a program that lives in that gap – informing a new generation with a modern method of leadership instruction. Through a one day conference, participating students should experience personal and professional growth. Not only will they grow in their communication, motivation, and managerial skills – but they will also put together a plan of action for when the conference concludes. Students will use this plan to address an issue in their city, county, or …

Disturbance Regimes And Management Strategies Of Mountain Ash Forest Ecosystems In Victoria, Australia; A Literature Review, Zoe Plumb

Sustainability and Social Justice

This paper discusses the ecology of mountain ash forests, the disturbances regimes that currently exist in these ecosystems, and finally addresses the current management practices and future management practices. Mountain ash forests are subjected to a wide range of research in the Central Highlands of Victoria, an area approximately 14,000 hectares in range. These forests are dominated by montane ash trees (Eucalyptus regnans F. Muell), which are critically endangered and at risk of collapse, attributed to the decline in large hollow-bearing trees throughout the region. Management of these forests are controlled by the Department of Environment, Land, Water, and …

An Investigation Of Resistance Training Behavior, Non-Specific Psychological Distress, And Perceived Barriers To Resistance Training In Self-Identifying Female Undergraduate Students, Jody Sobel

Master's Theses

The prevalence and severity of depression symptoms amongst college students, specifically female identifying undergraduate students, is worrisome. There is a great deal of research that suggests a relationship between exercise, specifically resistance training (RT), and mental health. That said, current literature suggests that many female identifying students are not engaging due to perceived barriers. This, perhaps because of the plethora of barriers one can perceive. The purpose of this study was multifold 1) to examine the prevalence and severity of non-specific psychological distress; 2) to determine if there were differences between Kessler 6 Non-Specific Psychological Distress Scale (K6) categories (i.e., …

Hawk Mountain Raptor Migration Phenology’S Relation To Weather, Eric Burgos

Computer Science and Information Technology Faculty

We have been studying year-round raptor migration phenology across the United States and North America for multiple decades now. Hawk Mountain Sanctuary’s Autumn migration hawk count began in 1934 and is the longest running raptor migration count in the world. A decline in total raptor counts passing through Hawk Mountain’s North Lookout is well documented and much research has already been done in what could be the main causes for this decrease in counts year-over-year. We know that cold front passages have long been associated with autumnal migration in northeastern North America. Using updated analysis techniques, we examined 60 years’ …

Country-Scale Crop Field Mapping For Zambia Using U-Net, Yao-Ting Yao

Sustainability and Social Justice

Cropland mapping is a crucial tool for evaluating food security. Cropland in African countries is expected to expand by at least 140M ha by 2050 to satisfy food demand. Developing accurate, large-area cropland maps of Africa’s smallholder agricultural systems is critical. To address this need, we use U-Net, a convolutional neural network, to map cropland for the year 2021 in the Republic of Zambia, a country experiencing rapid agricultural growth. To undertake this work, we first created a labeling platform and workflow protocol on Google Earth Engine (GEE) to collect labels from high spatial-resolution Planet-Scope NICFI Basemaps (4.77m) within grids …

Upcycling Imperfect Broccoli And Carrot To Healthy Snacks Using An Innovative 3d Food Printing Approach, Taylor Clary

Food Science Undergraduate Honors Theses

Vegetables are healthy foods with nutritional benefits; however, nearly one-third of the world’s fruits and vegetables are lost each year. The losses have the same nutritional value as an acceptable vegetable but have odd shapes or blemishes that make them unsuitable to consumers. 3D technology has numerous capabilities in the automotive, healthcare, and, now, food industries. Within the food industry, 3D food printing can bring customization to product design and nutrition as well as other advancements, including decreasing food waste. In this study, imperfect vegetables (i.e., broccoli and carrot) were upcycled into freeze-dried powders to improve their shelf-life before they …

Student Preferences On The Development Of A Non-Alcoholic Beverage Product To Alleviate The Negative Impact Of Alcohol On College Campuses, Cecilia Azar

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Alcohol abuse and peer pressure to drink are prominent issues on college campuses across the nation. More than 50 percent of college students aged 18 to 22 consume alcohol monthly and 33 percent consistently engage in binge drinking (National Institute on Alcohol Abuse and Alcoholism, 2021a). A non-alcoholic beverage product marketed to students as an alternative to alcohol in social settings could alleviate some of the negative impacts, such as peer pressure and the dangers caused by overconsumption.

Through two phases, this study implements University of Arkansas student preferences and recipe modification in the development of a non-alcoholic beverage product …