Life Sciences Commons^™

Older Adult Aerobic Capacity, Muscular Strength, Fitness And Body Composition After 20+ Years Of Exercise Training: A Systematic Review And Meta-Analysis, Eric Leslie, Vincent Luna, Ann L. Gibson

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 620-637, 2023. Older adults (³ 65 years) are recommended to participate in regular exercise to maintain health in late adulthood. The impact of long-term (20+ years) exercise training that align with the American College of Sports Medicine’s (ACSM) recommended guidelines has not been evaluated for older adults. To address this, a systematic review and meta-analyses were performed regarding the effects of long-term exercise training on older adult aerobic capacity, muscular fitness, and body composition that meet the ACSM’s recommendation for weekly training volume. Ten studies with individuals that performed cardiorespiratory or resistance exercise met …

Immediate Effect Of Gluteus Medius Kinesio-Taping On Plantar Pressure Distribution And Balance Among Healthy Individuals, Deepa Shekar, Hemant K. Kalyan, Prem Venkatesan, Nithin Prakash, Karvannan Harikesavan

International Journal of Exercise Science

International Journal of Exercise Science 16(1): 587-598, 2023. Gluteus medius eccentrically regulates hip adduction and internal rotation in unilateral postures against gravity. Any weakness to Gluteus medius can lead to poor posture, impaired balance and altered plantar pressure. There is a scarcity of literature to find the immediate effect of gluteus medius kinesio-taping on plantar pressure distribution and balance among healthy individuals. A Randomized cross-over trial was conducted in outpatient physiotherapy department of Manipal Hospitals Bangalore, on 28 healthy individuals from March 2021 to April 2022. The taping was done on the dominant leg of the subjects, with no tape, …

Variation In Sign-Tracking And Goal-Tracking Behaviors In A Genetically Diverse Inbred Panel Of Mice, Emily A. Schoenblum

Undergraduate Honors Theses

No abstract provided.

On The Resurrection Of Microbes: An Eco-Christological Approach To The Resurrection, Denys Janiga Osb

Obsculta

This article attempts to bring ecology and Christology into conversation through a transdisciplinary approach. It looks at the pastoral implications of eco-anxiety, resurrection ecology in the field of biotechnology, Church teaching on the Resurrection, and concludes by initiating an interpretation of the resurrection appearances in the Gospel of Luke through the lens of the microbiome.

Cannabidiol As A Synergist In Chemotherapy, Liam Pontes

Honors Program Theses and Projects

Cannabidiol (CBD) is a non-psychoactive compound commonly found in hemp and non-hemp plants. It has a molecular effect on cells, but its effect in modifying cellular signaling pathways has not been thoroughly tested until recently. Experimentation was done to determine whether CBD could induce cell death in MCF7 breast cancer cells. Both normal MCF7 cells and Cisplatin resistant MCF7 cells were used for these experiments. The cells were dosed with 0, 0.5, and 1.0 g/ml CBD dissolved in methanol. Later micrographs of the treated cells were collected and Annexin staining to determine the effect of CBD on cell death was …

Appearance Of Choline Metabolites In Plasma And Milk When Choline Is Infused Into The Abomasum With Or Without Methionine, C. J.R. Jenkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Four lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metabolites and plasma AA in cows fed a Met-deficient diet. Cows were randomly assigned to 1 of 4 experimental treatments: (1) control; no supplemental Met or choline (CON), (2) 13 g/d of choline ion delivered via abomasal infusion (CHO), (3) 13 g/d of Met delivered via abomasal …

Differential Degeneration Of Neurons In A Mouse Model Of Canavan Disease, Vibha Chauhan, Quy Nguyen, Jeremy Francis, Paola Leone

Rowan-Virtua Research Day

Canavan disease (CD) is an inherited leukodystrophy caused by inactivating mutations to the glial enzyme aspartoacylase (ASPA). ASPA catabolizes neuronal N-acetylaspartate (NAA) into free acetate and aspartate and loss of this function results in the chronic elevation of non-catabolized NAA and the failure of developmental myelination. Elevated NAA is thought to cause damage to myelin and myelin-producing cells (oligodendrocytes, but the viability of neurons in CD is relatively unexplored. We compare here the progressive degeneration of neurons in two regions of the CD mouse brain, the thalamus and the cortex, distinguished by differing degrees of vacuolation, and show that the …

The Involvement Of Ubiquitin In Med13 Cyclin C Degradation Following Cellular Stress, Ayesha Gurnani, Brittany Friedson, Katrina Cooper

Rowan-Virtua Research Day

The Cdk8 Kinase Module is a dissociable regulator of cellular stress response genes, with degradation of its components Med13 and cyclin C eventually determining cell fate decisions such as engaging cell survival or cell death mechanisms. We aimed to explore the roles of ubiquitin in degradation of the Cdk8 Kinase Module following nitrogen starvation, with respect to the potential involvement of deubiquitinating enzyme Doa4, lysine linkage at position K63, and E2 ubiquitin conjugating enzymes Ubc4 and Ubc5. We utilized Western blot analysis to observe nitrogen starvation-induced degradation of Med13-HA in wild-type, doa4 mutant, and K63R yeast strains; degradation of cyclin …

Comparative Analysis Of The Effects Of Actual Versus Assumed Opioid Experience On The Regulation Of Ventral Striatal Opioid Receptor Gene Expression, Indu Mithra Madhuranthakam, Martin Job

Rowan-Virtua Research Day

Rationale: We conducted experiments to assess the effect of prior opioid experience on gene expression changes. We compared the current experimenter-imposed short versus extended-access conditions of opioid self-administration and developed a new quantitative method to determine their effectiveness in identifying the role of opioid experience in regulating opioid receptor expression levels in the ventral striatum (VS) using an oxycodone self-administration/abstinence model.

Methods: In this study, male Sprague-Dawley rats (n=36) were trained for 20 days to self-administer oxycodone at 0.1 mg/kg/infusion under short access (n=15, or saline as controls n=3, for 3h/day) or extended access (n=15, or saline as controls n=3, …

Identifying Co-Factors That Drive Tra-1 Activator Function, Jibran Imtiaz, Yongquan Shen, Ronald Ellis

Rowan-Virtua Research Day

Gli proteins are involved in cell fate determination, proliferation, and patterning in many species and are major effectors of Hedgehog (Hh) signaling. There are three Gli proteins in humans, and mutations or errors in their regulation lead to a variety of developmental disorders or cancer. However, the mechanisms by which they interact with co-factors are poorly understood. We are analyzing co-factors of Gli proteins using TRA-1 in Caenorhabditis nematodes. The TRA-1 zinc fingers are structurally like those of other Gli proteins, and TRA-1 can be cleaved like other Gli proteins to form a repressor. However, its function has changed during …

Swallowing Disrupts Tongue-Jaw Coordination During Chewing In A Rat Model Of Parkinson's Disease, Meejan Palhang, N. Charles, Francois Gould

Rowan-Virtua Research Day

The primary motor symptoms of Parkinson’s disease, including bradykinesia, rigidity, and tremor, are associated with difficulties regulating transitions between motor behaviors due to basal ganglia dysfunction. Chewing and swallowing, which are disordered in most patients with Parkinson’s disease, are two complex motor behaviors which overlap in time and share some neuromuscular components. The objective of this study is to identify how Parkinson’s disease affects the coordination of chewing and swallowing. We hypothesize that as a result of impaired regulation of shift between motor patterns, chewing cycles that occur with a swallow will be more affected that chewing cycles occurring in …

Perinatal Experiences Of People With Autism Spectrum Disorders: A Preliminary Scoping Review, Tanvi Shah, Andrea Iannuzzelli, Venkateswar Venkataman

Rowan-Virtua Research Day

Autism Spectrum Disorder (ASD) is a developmental disorder that is diagnosed in early childhood, typically in people who are assigned male at birth. However, this diagnosis and the sensory and behavioral divergence that comes with ASD stay with people throughout their lives. Based on the DSM-V diagnostic criteria and the history of how Autism and Autism Spectrum Disorders were first identified, many people who do not identify as male and/or present atypically do not receive a formal diagnosis until later in life or self-diagnose. This in turn has led to a lack of research in both populations that do not …

Pursuing Antiracist Public Policy Education: An Example Connecting The Racist History Of Housing Policy To Contemporary Inequity, Craig W. Carpenter, Tyler Augst, Harmony Fierke-Gmazel, Bradley Neumann, Richard Wooten

Journal of Extension

We review the antiracism concept and contextualize it in Extension public policy education and the Extension system itself. Despite public policy education having a long history in Extension on a wide variety of issues, missing from this programming is the pursuit of antiracism. As a programmatic example, we review some historical causes of present-day housing inequities and an associated example approach for pursuing antiracism in housing policy education. Finally, we conclude by noting additional opportunities to pursue antiracism in Extension public policy education. In doing so, we emphasize that public policy education cannot be “nonracist” if it is not antiracist.

Shiny Apps: The Evolution Of Extension Tools From Spreadsheets To New Interactive Dashboards, Allan Fabricio Pinto Padilla, Terry Wayne Griffin

Journal of Extension

The Interactive web dashboard is the newness with characteristics and features that are replacing the status quo downloading spreadsheets. RStudio is the program used to create Shiny Apps dashboards with R. We explain some of those features so that Extension specialists can adopt the methodology, engage stakeholders, and help them understand and apply results to their production systems, although we do not explain the creation process step by step. Our approach offers not only a useful and modern way to disseminate information to empower decision-making, but also a safe environment in which data can be automatically updated and users cannot …

Virtually The Same? Understanding Virtual And F2f Farmer Audiences, Laura Witzling, Eric Williams, Dara M. Wald, Jacqueline Comito, Elizabeth Ripley

Journal of Extension

Agricultural and extension educators frequently employ a variety of methods to provide farmers with information about conservation practices. The introduction of virtual programming brought on in response to the COVID-19 pandemic set the stage for analysis of farmer outreach preferences with respect to face-to-face (F2F) versus virtual outreach. Using survey data of individuals who participated in field days in Iowa, we segmented participants based on their F2F or virtual attendance. We compared the groups based on key variables such as water quality concerns, communication behaviors, outreach preferences, and demographics. Our work suggests that a broad and dynamic communication strategy, including …

Research Personnel Exposure To Carbon Dioxide During Euthanasia Procedures, Devon Nealer, Felicia Reibstein, Jennifer Bendorf, Emily Lafferty, Stephanie Ramborger, Taylor Swift, Jacqueline O'Malley, Thomas Boyle

Rowan-Virtua Research Day

Carbon dioxide (CO2) is a commonly used euthanasia agent in animal facilities. This procedure is carried out by replacing oxygen with carbon dioxide in animal cages, thus providing a quick and painless method of euthanasia. Unfortunately, there has been limited research on the potential effects of human exposure to CO2 during euthanasia procedures.

Following a previous carbon dioxide exposure study in the vivarium at Rowan SOM in 2016, the CO2 cylinders and euthanasia chambers have been relocated to room 153 Science Center and the vivarium has adopted a localized exhaust that helps remove the contaminant at the source.

While the …

Orthologs Of The C. Elegans Heterochronic Genes Have Divergent Functions In C. Briggsae, Maria Ivanova, Eric G. Moss

Rowan-Virtua Research Day

The heterochronic genes of C. elegans comprise the best-studied pathway controlling the timing of tissue and organ formation in an animal. To begin to understand the evolution of this pathway, the significance of each factor, and the relationships among the components, we characterized 11 C. briggsae orthologs of C. elegans heterochronic genes. Using CRISPR/Cas9, we made a variety of alleles and found that several mutant phenotypes differ in significant ways from those of C. elegans. Although most orthologs displayed defects in developmental timing, those phenotypes could differ in which stages they controlled, the penetrance and expressivity of the phenotypes, or …

Extravasated Brain-Reactive Autoantibodies Perturb Neuronal Surface Protein Expression In Alzheimer's Pathology, Wardah Bajwa, Mary Kosciuk, Randel L. Swanson, Anuradha Krishnan, Venkat Venkataraman, Robert Nagele, Nimish Acharya

Rowan-Virtua Research Day

Background: Increased blood-brain barrier (BBB) permeability is reported in both the neuropathological and in vivo studies in both Alzheimer’s Disease (AD) and age matched cognitively normal, no cognitive impairment (NCI), subjects. Impaired BBB allows various vascular components such as immunoglobulin G (IgG) to extravasate into the brain and specifically bind to various neuronal surface proteins (NSP), also known as brain reactive autoantibodies (BrABs). This interaction is predicted to further enhance deposition of amyloid plaques.

Hypothesis: Interaction between extravasated BrABs and its cognate NSPs lower the expression of that NSPs in AD patients.

Methods: We selected Western blotting technique to study …

Proximate Causes Of Alternative Reproductive Tactics In Blue Ridge Two-Lined Salamanders (Eurycea Cf. Wilderae), Yatin Kalki

Master of Science in Integrative Biology Theses

Alternative reproductive tactics (ARTs) refer to discrete variation in reproductive behavior between members of a sex and within a species. ARTs are present in Blue Ridge two-lined salamanders (Eurcyea cf. wilderae) in the form of male polymorphism. Two male morphs exist: “searching” males and “guarding” males. Each phenotype has a unique reproductive ecology and associated discrete morphological differences. Another trait that shows discrete variation in E. cf. wilderae is the larval period, which may be one or two years in length. This thesis explores the proximate causes of ARTs in a population of E. cf. wilderae from the …

Improvement Of A Low-Cost Diy Wave Gauge, Matthew F. Virden, Nigel A. Temple, Bret M. Webb, Eric L. Sparks

Journal of Extension

The impacts of waves on shorelines and nearshore ecosystems has highlighted the need for extension and other environmental professionals to have access to accurate and affordable wave measurements. The development of a low-cost DIY wave gauge improved the accessibility of these measurements; however, the original design was limited in battery life. Here, an improved version of the low-cost DIY wave gauge, the DIY Feather Wave Gauge, is presented with the same performance, longer battery life, smaller design, and cheaper cost along with tutorials, parts lists, and other resources. This new gauge has been used to improve shoreline management recommendations.

Linking Social And Built Environmental Factors To Leisure-Time Physical Activity In Rural Cancer Survivors, Courtney J Stevens, Yue Liao, Minxing Chen, Natalia I Heredia, Hannah Arem, Jasmine Sukumar, Lenat Joffe, Kathryn H Schmitz, Scherezade K Mama

Faculty, Staff and Student Publications

BACKGROUND: This study explored associations between social and built environmental factors and leisure-time physical activity (LTPA) in rural cancer survivors (RCS) and whether these associations differed by exercise stage of change (SOC).

METHOD: RCS (n = 219) completed questionnaires assessing LTPA, SOC, and social (social status, connectedness, support) and environmental (home environment, neighborhood environment) factors. Linear regression models examined associations between social and built environmental factors and LTPA and tested for moderation by SOC.

RESULTS: Half (50.7%) of RCS were physically active, and 49.3% were not active. Social factors positively associated with LTPA included subjective social status in the community …

Editorial: Gulf Of Mexico Estuaries: Ecology Of The Nearshore And Coastal Ecosystems Impacted By The Deepwater Horizon Oil Spill, Charles W. Martin, Paola C. López-Duarte, Jill A. Olin, Brian J. Roberts

Michigan Tech Publications, Part 1

No abstract provided.

Twas_Sim, A Python-Based Tool For Simulation And Power Analysis Of Transcriptome-Wide Association Analysis, Xinran Wang, Zeyun Lu, Arjun Bhattacharya, Bogdan Pasaniuc, Nicholas Mancuso

Faculty, Staff and Student Publications

Genome-wide association studies (GWASs) have identified numerous genetic variants associated with complex disease risk; however, most of these associations are non-coding, complicating identifying their proximal target gene. Transcriptome-wide association studies (TWASs) have been proposed to mitigate this gap by integrating expression quantitative trait loci (eQTL) data with GWAS data. Numerous methodological advancements have been made for TWAS, yet each approach requires ad hoc simulations to demonstrate feasibility. Here, we present twas_sim, a computationally scalable and easily extendable tool for simplified performance evaluation and power analysis for TWAS methods.

Software and documentation are available at https://github.com/mancusolab/twas_sim.

Toward The Adoption Of New Farming Systems Among Farmers: A Case Study Of Short Rotation Woody Crops In North Carolina, Omoyemeh J. Ile, Eli Typhina, Katie Brannum, Rajan Parajuli, Robert E. Bardon, John S. King

Journal of Extension

This study explores the human dimensions of the broad-based adoption of Short Rotation Woody Crops (SRWCs) among farmers in North Carolina. We used an actor diagramming and tracing approach to explore factors influencing farmers’ adoption of SRWCs. Results suggest four factors strongly influence the adoption process: 1) market availability, 2) education awareness, 3) funding, and 4) social networking. Based on these results, we recommend that Extension professionals use the following education modules to prompt the adoption of SRWCs practices and potentially adopt other new farming practices: 1) ecological sustainability, 2) financial considerations, 3) harvesting, and 4) community building.

Dimeric P53 Mutant Elicits Unique Tumor-Suppressive Activities Through An Altered Metabolic Program, Jovanka Gencel-Augusto, Xiaoping Su, Yuan Qi, Elizabeth M Whitley, Vinod Pant, Shunbin Xiong, Vrutant Shah, Jerome Lin, Encarnacion Perez, Marta L Fiorotto, Iqbal Mahmud, Abhinav K Jain, Philip L Lorenzi, Nicholas E Navin, Ellen R Richie, Guillermina Lozano

Faculty, Staff and Student Publications

Cancer-related alterations of the p53 tetramerization domain (TD) abrogate wild-type (WT) p53 function. They result in a protein that preferentially forms monomers or dimers, which are also normal p53 states under basal cellular conditions. However, their physiologic relevance is not well understood. We have established in vivo models for monomeric and dimeric p53, which model Li-Fraumeni syndrome patients with germline p53 TD alterations. p53 monomers are inactive forms of the protein. Unexpectedly, p53 dimers conferred some tumor suppression that is not mediated by canonical WT p53 activities. p53 dimers upregulate the PPAR pathway. These activities are associated with lower prevalence …

Educational Needs Of North Carolina Non-Industrial Private Forest Landowners And Barriers To Meeting These Needs, Robert E. Bardon, Kristin Peters, Rajan Parajuli, K.S.U. Jayaratne

Journal of Extension

Forest landowners are an important target audience for many state Extension programs. Acknowledging the differences and associations between landownership values, characteristics, and educational preferences of forest landowners should lead to improvement of educational programs and ensuring that educational needs are being met. Through an internet-based survey of forest landowners four distinct landowner typologies were identified based on respondents’ reason for owning forestland. Results also identified the educational needs and barriers to meeting these needs for the landowners. Creating typologies based on attitudinal responses will allow for a more focused approach to developing educational products and services to meet landowner needs.

A Small-Scale Wave Tank For Living Shoreline Extension And Outreach, Keith Chenier Jr, Jaden Akers, Skylar Liner, Shelby Harrier, Eric Sparks, Patrick Biber, Laura Blackmon, Jessica Kastler, Patrick Broussard

Journal of Extension

Hardened structures, such as bulkheads and seawalls, are currently the primary choice of shoreline protection for waterfront property owners. However, hardened shorelines are known to enhance erosion and often promote the loss of intertidal habitat. Living shorelines are a collection of environmentally friendly construction techniques that serve as an alternative to hardened shorelines through the incorporation of natural materials, such as native plants. To enhance extension and outreach efforts related to the promotion of living shorelines, we created a small-scale, hands-on wave tank that demonstrates the benefits of natural and living shorelines relative to hardened structures. This interactive tool allows …

Qualitative Analysis Of The Expanded Food And Nutrition Education Program’S 24-Hour Dietary Recall, Serena M. Fuller, Josh A. Phelps, Susan Baker, Jennifer Walsh

Journal of Extension

The Expanded Food and Nutrition Education Program (EFNEP) uses a group 24-hour dietary recall (Gr24HDR) to measure changes in diet quality. Participant perceptions of the tool can guide implementation practices used by EFNEP Extension staff. Focus group (FG) sessions were conducted in five states and transcripts analyzed following a framework analysis approach. According to FG participants, a range of factors, condensed into six themes with potential interactions, influence Gr24HDR including implementation processes and community settings. Findings inform overarching considerations EFNEP staff may take when collecting Gr24HDR data in the field to improve the evaluation process for participants.

A Vlp-Based Vaccine Displaying Hbha And Mtp Antigens Of Mycobacterium Tuberculosis Induces Potentially Protective Immune Responses In M Tuberculosis H37ra Infected Mice, Juan Wang, Tao Xie, Inayat Ullah, Youjun Mi, Xiaoping Li, Yang Gong, Pu He, Yuqi Liu, Fei Li, Jixi Li, Zengjun Lu, Bingdong Zhu

Faculty, Staff and Student Publications

Heparin-binding hemagglutinin (HBHA) and M. tuberculosis pili (MTP) are important antigens on the surface of Mycobacterium tuberculosis. To display these antigens effectively, the fusion protein HBHA-MTP with a molecular weight of 20 kD (L20) was inserted into the receptor-binding hemagglutinin (HA) fragment of influenza virus and was expressed along with matrix protein M1 in Sf9 insect cells to generate influenza virus-like particles (LV20 in short). The results showed that the insertion of L20 into the envelope of the influenza virus did not affect the self-assembly and morphology of LV20 VLPs. The expression of L20 was successfully verified by transmission …

Insights Into The Formation And Diversification Of A Novel Chiropteran Wing Membrane From Embryonic Development, Neal Anthwal, Daniel J Urban, Alexa Sadier, Risa Takenaka, Simon Spiro, Nancy Simmons, Richard R Behringer, Chris J Cretekos, John J Rasweiler, Karen E Sears

Faculty, Staff and Student Publications

BACKGROUND: Through the evolution of novel wing structures, bats (Order Chiroptera) became the only mammalian group to achieve powered flight. This achievement preceded the massive adaptive radiation of bats into diverse ecological niches. We investigate some of the developmental processes that underlie the origin and subsequent diversification of one of the novel membranes of the bat wing: the plagiopatagium, which connects the fore- and hind limb in all bat species.

RESULTS: Our results suggest that the plagiopatagium initially arises through novel outgrowths from the body flank that subsequently merge with the limbs to generate the wing airfoil. Our findings further …