Life Sciences Commons^™

The Effects Of Aloe Vera On The Human Gut Microbiome, Samantha Leon

Honors Theses

The human gut microbiome is a diverse community of bacteria influenced by multiple factors including host age, geographical location, and diet. This study observed the effects of aloe vera juice on the human gut microbiome. Fecal samples were taken every two weeks over a period of one year; the subject consumed aloe vera juice in alternating cycles of consumption “on” and abstaining “off” with each cycle lasting for eight weeks. The bacterial community in each sample was characterized using 16S rRNA gene sequencing. Sequences were identified and their relative abundances were used to look for patterns distinguishing “on” and “off” …

Structural Integrity And Stability Of Dna In Ionic Liquid And Near-Infrared Indolizine Squaraine Dye, Ember Yeji Suh

Honors Theses

Luminol, the most common presumptive test for blood at a crime scene, has multiple issues, such as false positive results with chemical agents, no luminescence due to “active oxygen” cleaning agents on bloodstains, and inability to penetrate textile materials. A combination of indolizine squaraine dye and ionic liquid (IL), or Dye Enhanced Textile Emission for Crime Tracking (DETECT), have shown potential to address these issues. The purpose of this study was to assess the binding mechanism of CG (1:1) and SO₃SQ dye to HSA and how the mechanism can explain the W214 fluorescence quenching effect and to determine …

A Camera Trap Study Of Scavenger Species On The University Of Mississippi Campus, Lise Larsen

Honors Theses

This is a camera trap study to understand what kind of mammalian scavenger community we have on the University of Mississippi campus and where they are most prominent. Three motion-triggered cameras were used to survey ten buildings on campus over ten weeks. A low number of individuals were caught on camera during this study. There were limitations on this study such as a low number of cameras and a short span of time. More research needs to be done to understand the scavenger community on this campus.

The Interhemispheric Auditory Tract Connectivity And Autism Spectrum Disorder, Josie Long

Honors Theses

Individuals with autism spectrum disorder (ASD) can have abnormal responses and hypersensitivity to sounds (Haesen et al., 2011). ASD is also related to inattentive, hyperactive, and impulsive behaviors (Bradley & Isaacs, 2006). One study by Stansberry et al. (2022) found that a higher fractional anisotropy (FA) of interhemispheric auditory white matter is associated with higher impulsivity. Our study examined the interhemispheric auditory tract (IAT) to determine whether it is impacted by ASD. We used diffusion tensor imaging (DTI) data from Autism Brain Imaging Data Exchange utilizing two datasets so that we could replicate primary findings. We first isolated the IAT …

An Analysis Of The Sensory Characteristics And Microbial Constituents Of Kefir, Katherine Quay Robinson

Honors Theses

Kefir is a fermented beverage produced using sugar water or milk, kefir grains, either water kefir grains or milk kefir grains, respectively, and microbial aggregates consisting of diverse bacteria and yeasts. As kefir and fermented beverages in general are increasing in popularity, research related to optimal sensory characteristics and consumer-approval is vital. Desirable sensory characteristics for kefir beverages include low sour taste/aroma, high sweetness, carbonation, and high viscosity (Surja et al., 2019; Margareth et al., 2020). Production methods of kefir can alter sensory characteristics, but sensory characteristics are likely strongly influenced by the microbial composition of the grains/product. Thus, an …

The Effects Of Acute Exercise Intensity On Memory: State-Dependent Learning, Lauren E. Fuglaar, Rylie S. Mangold, Sierra N. Petty

Honors Theses

The Encoding-Specificity Paradigm states that memory recall will be enhanced when contextual factors are congruent between memory encoding and memory retrieval (i.e., state-dependent learning). However, minimal research has evaluated this paradigm in the exercise domain. The purpose of this study was to evaluate the effects of acute exercise intensity on memory while considering these potential state-dependent effects. Forty-eight university students completed a within-subject experiment involving seven laboratory visits, during which they encoded a list of 15 words and were subsequently asked to free-recall as many of these words as possible. The encoding and retrieval phases were matched or mismatched by …

Photocatalyzed Hydrogen Evolution Using Nickel-Bound Metallothionein Protein, Windfield Swetman

Honors Theses

The continued burning of fossil fuels is not only a cause of increasing deterioration of the environment but also a financially unsustainable source of energy. Advances in energy production must be investigated to avoid the long-term effects of this current main source of energy. One of the avenues being explored is the use of metalloenzymes to catalyze hydrogen evolution via water splitting that occurs during the reductive half of artificial photosynthesis. Metalloenzyme catalysts with a single Ni(Cys)₄ active site have been previously studied, and this study explores the possibility of increasing hydrogen production by using metalloenzyme catalysts with multiple …

Effectiveness Of Tadreb-B1 And 1-Feh W3 Kasp Markers In Spring And Winter Wheat Populations For Marker-Assisted Selection To Improve Drought Tolerance, Shamseldeen Eltaher, Mostafa Hashem, Asmaa A. M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Due to the advances in DNA markers, kompetitive allele-specific PCR (KASP) markers could accelerate breeding programs and genetically improve drought tolerance. Two previously reported KASP markers, TaDreb-B1 and 1-FEH w3, were investigated in this study for the markerassisted selection (MAS) of drought tolerance. Two highly diverse spring and winter wheat populations were genotyped using these two KASP markers. The same populations were evaluated for drought tolerance at seedling (drought stress) and reproductive (normal and drought stress) growth stages. The single-marker analysis revealed a high significant association between the target allele of 1-FEH w3 and drought susceptibility in the spring …

Barriers To Exercise In A Medical Unit Of Us Army Reservists: An Exploratory Study, Madelyn B. Huhn, Elizabeth A. Kleitz, Namuna Sunar

Undergraduate Research Journal for the Human Sciences

Military Reservist healthcare workers must meet physical fitness standards similar to those on active duty. They, however, experience a unique occupational environment as citizen Soldiers. Their civilian careers as healthcare workers and their positions in the military both demand shift work, long hours, and critical response to high stress events. For a small group of Reservists working in civilian healthcare occupations who also serve in medical roles in the military, this exploratory study aimed to describe any perceived barriers to exercise. Although over 60% of respondents to an exercise barriers scale perceived physical and social health as the top benefits …

Smokejumper Obituary: Young, Austin L. (Mccall 1954), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Embracing Ai/Ml In Genetic Counseling: A Nationwide Survey On Program Leaderships’ Perspectives And Curriculum Integration, Kyla Holmes

KGI Theses and Dissertations

This study aimed to examine the attitudes and preparedness of genetic counseling program directors and faculty leadership in incorporating artificial intelligence and machine learning (AI/ML) into their curricula and its effect on core competency proficiency. AI/ML has been instrumental in creating and maintaining vital analytical tools and models employed by genetic counselors (GCs). However, research on the attitudes of faculty leadership in charge of training future GCs is limited. A nationwide survey conducted between November 2022 and February 2023 gathered 15 respondents holding diverse academic positions in genetic counseling program curriculum development. The majority of respondents had encountered AI/ML in …

The Development Of A Primer Payload With Microparticles For Uti Pathogen Identification Using Polythymidine- Modified Lamp Primers In Droplet Lamp, Jonas Otoo

KGI Theses and Dissertations

Nucleic acid amplification tests (NAATs) are among the diagnostic tests with the highest sensitivity and specificity. However, they are more complex to develop than other diagnostic tests such as biochemical tests and lateral flow immunoassay tests. Polymerase chain reaction (PCR) is the gold standard for NAATs. PCR requires thermal cycling to achieve clonal amplification of the target pathogen DNA for diagnosis. Thermal cycling poses a challenge in the development of PCR diagnostics for point-of-care (POC) settings. Loop-mediated isothermal amplification (LAMP) offers an isothermal method for NAATs diagnostics. The advancement of the microfluidics field significantly enhances the development of LAMP diagnostics …

The Effect Of Shrub Cover On Conifer Water-Use Patterns, Growth, And Response To Climate Change In The Southern Sierra Nevada, Marissa Goodwin

Biology ETDs

As wildfires increase in size and severity, large areas of forest are undergoing substantial increases in shrub cover. In forests where water is the limiting resource, the partitioning of soil water between shrubs and trees may determine how shrubs affect tree growth and water-stress. We analyzed the δ²H and δ¹⁸O values of xylem water for two conifer species (Abies concolor and Pinus jeffreyi) and two shrub species (Arctostaphylos patula and Ceanothus cordulatus) to assess how shrub cover affects the water-uptake patterns of conifers in the southern Sierra Nevada. We found that growing …

Protein Dynamics In Allostery And Computer-Aided Drug Design, Mayar Mohamed

Chemistry Theses and Dissertations

Molecular dynamics (MD) simulation is a powerful and versatile computational tool, that can be used in a wide range of applications from studying complex biological processes such as allostery to investigating the process of the protein-ligand binding in different computer-aided drug design implementations. In this work, various structural and drug discovery projects were performed by combining the state-of-art techniques molecular dynamics simulations, virtual screening, and binding free energy calculations with the innovative Markov State Model analysis and the machine learning techniques.

In the first project, the role of A’a helix as a key player in the transition between the light …

Eco-Interoception: What Plants, Fungi And Protista Have Taught My Body, Sara Riley Dotterer

Art Theses and Dissertations

To me, ecology is the relational, full-body awareness that I am made up of and deeply connected to everything around me; and for better or worse, this is reciprocal. I form ecotones, an ecological transitional zone between two ecosystems, with the world around me. I use this ecotonal lens to blur binaries and dissolve boundaries between me and the world “outside my body.” During my Masters of Fine Arts at Southern Methodist University, I have continuously explored and represented the lives of various more-than-human species outside of my body, including plants, fungi and protista through an ecotonal lens. Although these …

The Effect Of Sleep Chronotype On Cardiorespiratory Fitness, Kimberly C. Bennett

Senior Honors Projects, 2020-current

Purpose: To examine the effect of sleep chronotype and sleep quality on one’s ability to perform a maximal treadmill test in the morning and evening.

Methods: 13 individuals [age = 20.08 ± 1.04 yr] completed three graded exercise tests (GXT) on a treadmill. The familiarization test was done first at any time of day. The experimental trials were labeled as the “morning GXT” and the “evening GXT”. Experimental tests, completed in random order, were performed 7-14 days following the familiarization test between 7:00 and 9:00 am for the morning GXT and between 6:00 and 8:00 pm for the …

Copper Chelation Via Beta-Alanine Extends Lifespan In A C. Elegans Model Of Alzheimer’S Disease, Arnulfo Pulido, Benjamin Hulbert, Hayleigh Giese, Sabrina Kurian, Rebbeca Rozhon, Michael Zambrano, Oscar Diaz, Mariam Abd, Madison Caputo, Daniel S. Kissel, Mallory A. Havens

Biology Department Faculty Articles

The leading hypothesis for Alzheimer’s Disease (AD) has traditionally focused on the aggregation of Amyloid-β into amyloid plaques. However, research has yet to definitively prove the role of the amyloid peptide in the pathology of the disease. Given that all therapeutics targeting amyloid plaques have failed in clinical trials, with one exception, there is a need to explore alternative approaches to treatment of this disease. Therefore, we examined other factors that are altered during AD pathogenesis. Many AD patients have dysregulation of metal ions, such as copper and zinc, in addition to accumulation of Amyloid-β. The interaction between Amyloid-β and …

Neuroinflammatory And Metabolomic Temporal Dynamics From Inhaled Wildfire Smoke With Attenuation Via Pharmacological Intervention, David Scieszka

Biomedical Sciences ETDs

Wildfire smoke is a global issue affecting nearly all humans on the surface of this Earth. Smoke dispersion from wildfires can span continents and cross oceans with amazing ease. Furthermore, total acres of land burned per year in the United States has roughly doubled since 1980, meaning this issue will persist for an indeterminate amount of time. Exposure to wildfire smoke is hazardous and promotes aging-associated events in the cardiopulmonary systems. Yet long- and short-term effects on the brain remain relatively unknown. From these, it is critically important to understand the neurological impacts from wildfire smoke exposure; to compare these …

Comparing Calculated And Perceived Energy Needs In Collegiate Student-Athletes, Lyndsey T. Reed

Honors Theses

The purpose of this study was to evaluate NCAA student-athletes' level of knowledge related to their energy needs. The participants were 75—42 male and 33 female—NCAA Division I student-athletes enrolled at the University of Mississippi (UM) from a variety of sports. In-person data collection took place at UM Athletics facilities by sports registered dietitians during team talks. The names of the athletes were used to find their pre-existing calculated energy, resting metabolic rate (RMR) and total energy expenditure (TEE) using the Nelson equation and the appropriate activity factor. Questionnaire and RMR/TEE data were analyzed and compared to assess knowledge related …

Development Of Recombinant Varicella-Zoster Virus Vaccines Expressing Sars-Cov-2 Antigens, Cody Wilson

Honors Theses

The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) continues to cause infections and deaths around the globe. Since its emergence, scientists have worked diligently to discover and produce vaccines to battle the virus. While they successfully created and distributed vaccines, the vaccines are not without flaw. These vaccines do not grant long term immunity, require multiple booster shots, and have not prevented the emergence of viral variants. In this study, the SARS-CoV-2 receptor binding domain (RBD), which is a key part of the spike protein (S), and nucleocapsid (N) genetic sequences were inserted into the live varicella …

Connecting Social And Ecological Systems In Small-Scale Fisheries In The Philippines, Sara Eisler Marriott

Dissertations

Nearly 50% of all marine fish capture in the Philippines is from artisanal fisheries, most of which is un- or under-reported. As in many emerging nations around the world, the Philippines cannot fully address overfishing by managing only half of the catch that comes from commercial fisheries. Marine reserves are a popular governance strategy for conservation and of growing interest for fisheries management. Many marine reserves in the Philippines, however, are not considered effective. In 2014, Rare, an international NGO, implemented a community-based management program to increase the effectiveness of the marine reserves, and while it found biomass increased, there …

Compatibility Between Native Mississippi Ectomycorrhizal Fungi And Native And Exotic Pine Hosts: Testing For Specificity And The Potential For Rapid Evolution, Hailey A. Long

Honors Theses

Pines are highly invasive trees that are commonly used in pine plantations throughout the Southern Hemisphere. However, the survival and subsequent invasion of pines in exotic environments are dependent on the obligate symbiosis between ectomycorrhizal (ECM) fungi and pines. ECM fungi vary in host specificity and compatibility from pine-specialist species in the genus Suillus to broad generalists, such as species within the genera Pisolithus and Scleroderma. In Experiment 1, we sought to understand how the geographic origin of ECM fungi native to the southeastern United States and two pine species (native southeastern United States Pinus taeda and exotic Pinus …

Quantitative And Qualitative Analysis Of Mutation In Pam-1 Of Model Organism Caenorhabditis Elegans., Jessica Stein, Jessica Stein

Honors College Theses

The pam-1 gene in the model roundworm Caenorhabditis elegans governs meiotic exit and establishment of cellular polarity in the single-celled C. elegans embryo. Mutation of the pam-1 gene results in reduced fertility and fecundity in adult C. elegans and disrupts the anatomy of the germinal gonad. The aim of this study is to qualitatively and quantitatively define the changes in the germline cells associated with mutations to the pam-1 gene. Specifically, we examined the stages of germ cell development within the gonads of adult worms, both wild-type and pam-1 compromised, and identified the changes in the length of the mitotic, …

Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi

Nanoscience and Microsystems ETDs

Although the exact cause of Alzheimer’s disease (AD) is still unknown, it is widely considered that the accumulation of amyloid plaques composed of the amyloid-β (Aβ) peptide in the brain is linked to neurodegeneration. Co-localization of viral DNA with Aβ plaques, the association of brain infection and AD, and research indicating the protective effect of Aβ against bacteria and fungi in mice and human cells have led to the hypothesis that Aβ expression and deposition may be central to its function as an antimicrobial peptide (AMP). In my thesis research, we seek to elucidate how Aβ functions as an AMP …

Density And Nest Success Of Shrub-Dependent Birds On Formerly Strip-Mined Lands, Luke Headings

Electronic Theses & Dissertations

As bird populations continue to decline across North America, it is important to understand the benefits that disturbed habitats can have for breeding birds. One of the major land disturbances and causes of habitat loss in the United States is surface mining, which often results in altered vegetative communities. The primary goal of this study was to evaluate the relationships between bird populations, habitat, previous and current land use, and densities of invasive plant species on formerly strip-mined land. Due to the proliferation of invasive shrub species in post-mined landscapes, we sought to determine the effects of post-mined habitat features …

Population Dynamics And Habitat Use By Gray Bats In An Anthropogenically Altered Landscape, Haley Price

Electronic Theses & Dissertations

Habitat loss resulting from land use change is among the greatest threats to biodiversity. Yet, in some cases, imperiled wildlife species may benefit from anthropogenic disturbance. The expansion of urbanization, agriculture, renewable energy, and mining practices impact conservation efforts for threatened and endangered bat species, and particularly species that are affected by white nose syndrome (WNS). The federally endangered gray bat (Myotis grisescens) is a cavernicolous species that is susceptible to WNS. Due to their unique roosting and foraging requirements, gray bats inhabit a small portion of the accessible caves within their geographic range. As habitat loss persists, …

The Elephant In The Garden, Ila France Porcher

Animal Sentience

The other commentators on Chapman & Huffman (2018) have pointed out in different ways that despite our biological nature, there is a widespread tendency for humans to believe that we are not only superior to animals, but that we are not animals at all. Alongside our denial of animal sentience and cognition, this has resulted in the denial of our own instinctive natures. It is this denial that is our error, for it is only by understanding our true natural heritage that we can begin to change the runaway path we are on.

Vi Energy-Efficient Memristor-Based Neuromorphic Computing Circuits And Systems For Radiation Detection Applications, Jorge Iván Canales Verdial

Electrical and Computer Engineering ETDs

Radionuclide spectroscopic sensor data is analyzed with minimal power consumption through the use of neuromorphic computing architectures. Memristor crossbars are harnessed as the computational substrate in this non-conventional computing platform and integrated with CMOS-based neurons to mimic the computational dynamics observed in the mammalian brain’s visual cortex. Functional prototypes using spiking sparse locally competitive approximations are presented. The architectures are evaluated for classification accuracy and energy efficiency. The proposed systems achieve a 90% true positive accuracy with a high-resolution detector and 86% with a low-resolution detector.

Dynamic Conformational Switching Underlies Tfiih Function In Transcription And Dna Repair And Impacts Genetic Diseases, Jina Yu, Chunli Yan, Thomas Dodd, Chi-Lin Tsai, John A Tainer, Susan E Tsutakawa, Ivaylo Ivanov

Faculty, Staff and Student Publications

Transcription factor IIH (TFIIH) is a protein assembly essential for transcription initiation and nucleotide excision repair (NER). Yet, understanding of the conformational switching underpinning these diverse TFIIH functions remains fragmentary. TFIIH mechanisms critically depend on two translocase subunits, XPB and XPD. To unravel their functions and regulation, we build cryo-EM based TFIIH models in transcription- and NER-competent states. Using simulations and graph-theoretical analysis methods, we reveal TFIIH's global motions, define TFIIH partitioning into dynamic communities and show how TFIIH reshapes itself and self-regulates depending on functional context. Our study uncovers an internal regulatory mechanism that switches XPB and XPD activities …

Mowing Cattail Cover To Increase Aquatic Vegetation Diversity On The Coeur D’Alene River Floodplain In Cataldo, Idaho, Makenna J. Tabino

2023 Symposium

The Schlepp Easement is a 400 acre wetland on the Coeur D’Alene River floodplain, near Cataldo, Idaho. The wetland has been restored to protect it from heavy metal pollution transported downstream from mining sites near Kellogg, Idaho, the location of the Bunker Hill EPA Superfund Site. This wetland was restored to provide safe habitats and feeding grounds for migratory waterfowl and to maintain wetland biodiversity. However, cattail is prone to becoming overdominant and outcompeting other plants, which greatly limits a wetland’s biodiversity. Our objectives were to test whether aquatic boat mowing can reduce cattail cover, improving species diversity and cover …