Life Sciences Commons^™

Genomic Analyses Of A Widespread Blueberry Virus In The United States, Daisy Stainton, Dan E.V. Villamor, Andrea Sierra Mejia, Ashish Srivastava, Dimitre Mollov, Robert R. Martin, Ioannis E. Tzanetakis

Entomology and Plant Pathology Faculty Publications and Presentations

Screening of blueberry accessions using high throughput sequencing revealed the presence of a new virus. Genomic structure and sequence are similar to that of nectarine stem pitting associated virus (NSPaV), a member of the genus Luteovirus, family Tombusviridae. The full genome of the new luteovirus, tentatively named blueberry virus L (BlVL), was characterized and analyzed. Similar to NSPaV, BlVL does not contain readily identifiable movement proteins in any of the seven isolates sequenced. More than 600 samples collected from five states were screened and 79% were found infected, making BlVL the most widespread blueberry virus in the United States.

A Nurse-Led Telehealth Program For Diabetes Foot Care: Feasibility And Usability Study, Jeffery Alford, Constance Johnson

Faculty, Staff and Student Publications

BACKGROUND: Diabetes mellitus can lead to severe and debilitating foot complications, such as infections, ulcerations, and amputations. Despite substantial progress in diabetes care, foot disease remains a major challenge in managing this chronic condition that causes serious health complications worldwide.

OBJECTIVE: The primary aim of this study was to examine the feasibility and usability of a telehealth program focused on preventive diabetes foot care. A secondary aim was to descriptively measure self-reported changes in diabetes knowledge, self-care, and foot care behaviors before and after participating in the program.

METHODS: The study used a single-arm, pre-post design in 2 large family …

Review Of Toxicity Of Agricultural Chemicals And Implications For Aquatic Fauna Of The Keep River, Samanatha Reynolds, David Morgan

Natural resources commissioned reports

This report was compiled for the Western Australian Department of Primary Industries and Regional Development (DPIRD) as part of the National Water Grid Authority (NWGA) project entitled “Managing water quality to enable future irrigation development in the Kimberley Region, WA”. The purpose of this report is to review the toxicity of farm chemicals (herbicides and insecticides) used by farm operators in the Keep River catchment and their potential impacts on the aquatic species in the river, in particular those species that are listed under the Environment Protection and Biodiversity Conservation Act 1999. It is designed to complement the report entitled …

Non-Homologous End-Joining Pathway Genotypes Significantly Associated With Nasopharyngeal Carcinoma Susceptibility, Chia-Wen Tsai, Liang-Chun Shih, Wen-Shin Chang, Che-Lun Hsu, Jie-Long He, Yun-Chi Wang, Da-Tian Bau

Faculty, Staff and Student Publications

Defects in the non-homologous end-joining (NHEJ) DNA repair pathway lead to genomic instability and carcinogenesis. However, the roles of individual NHEJ genes in nasopharyngeal carcinoma (NPC) etiology are not well-understood. The aim of this study was to assess the contribution of NHEJ genotypes, including XRCC4 (rs6869366, rs3734091, rs28360071, rs28360317, rs1805377), XRCC5 (rs828907, rs11685387, rs9288518), XRCC6 (rs5751129, rs2267437, rs132770, rs132774), XRCC7 rs7003908, and Ligase4 rs1805388, to NPC risk, with 208 NPC patients and 416 controls. Genotype-phenotype correlations were also investigated by measuring mRNA and protein expression in adjacent normal tissues and assessing the NHEJ repair capacity in blood lymphocytes from 43 …

Achondroplasia Natural History Study (Clarity): 60-Year Experience In Orthopedic Surgery From Four Skeletal Dysplasia Centers, Nickolas J Nahm, W G Stuart Mackenzie, William G Mackenzie, Ethan Gough, S Shahrukh Hashmi, Janet M Legare, Peggy Modaff, Richard M Pauli, David F Rodriguez-Buritica, Maria Elena Serna, Cory J Smid, Julie Hoover-Fong, Michael B Bober

Faculty, Staff and Student Publications

BACKGROUND: The purpose of this study was to describe the frequency and risk factors for orthopedic surgery in patients with achondroplasia. CLARITY (The Achondroplasia Natural History Study) includes clinical data from achondroplasia patients receiving treatment at four skeletal dysplasia centers in the United States from 1957 to 2018. Data were entered and stored in a Research Electronic Data Capture (REDCap) database.

RESULTS: Information from one thousand three hundred and seventy-four patients with achondroplasia were included in this study. Four hundred and eight (29.7%) patients had at least one orthopedic surgery during their lifetime and 299 (21.8%) patients underwent multiple procedures. …

17Β-Estradiol Promotes Extracellular Vesicle Release And Selective Mirna Loading In Erα-Positive Breast Cancer, Rares Drula, Barbara Pardini, Xiao Fu, Mireia Cruz De Los Santos, Ancuta Jurj, Sherien M El-Daly, Erik Knutsen, Mihnea P Dragomir, Recep Bayraktar, Yongfeng Li, Meng Chen, Filippo Del Vecchio, Léa Berland, Jessica Dae, Daniel Fan, Masayoshi Shimizu, Anh M Tran, Mercedes Barzi, Carlotta Pioppini, Angelica M Gutierrez, Cristina Ivan, Salyna Meas, Carolyn S Hall, Suresh K Alahari, Ioana Berindan-Neagoe, Muller Fabbri, Anthony Lucci, Banu Arun, Simone Anfossi, George A Calin

Faculty, Staff and Student Publications

The causes and consequences of abnormal biogenesis of extracellular vesicles (EVs) are not yet well understood in malignancies, including in breast cancers (BCs). Given the hormonal signaling dependence of estrogen receptor-positive (ER+) BC, we hypothesized that 17β-estradiol (estrogen) might influence EV production and microRNA (miRNA) loading. We report that physiological doses of 17β-estradiol promote EV secretion specifically from ER+ BC cells via inhibition of miR-149-5p, hindering its regulatory activity on SP1, a transcription factor that regulates the EV biogenesis factor nSMase2. Additionally, miR-149-5p downregulation promotes hnRNPA1 expression, responsible for the loading of let-7's miRNAs into EVs. In multiple patient cohorts, …

Targeting Cxcr4 Abrogates Resistance To Trastuzumab By Blocking Cell Cycle Progression And Synergizes With Docetaxel In Breast Cancer Treatment, Shuying Liu, Shelly M Xie, Wenbin Liu, Mihai Gagea, Ariella B Hanker, Akshara Singareeka Raghavendra, Fuliang Chu, Sattva S Neelapu, Adriano Marchese, Samir Hanash, Johann Zimmermann, Carlos L Arteaga, Debasish Tripathy

Faculty, Staff and Student Publications

Background: Although trastuzumab and other HER2-targeted therapies have significantly improved survival in patients with HER2 overexpressed or amplified (HER2+) breast cancer, a significant proportion of patients do not respond or eventually develop clinical resistance. Strategies to reverse trastuzumab resistance remain a high clinical priority. We were the first to report the role of CXCR4 in trastuzumab resistance. The present study aims to explore the therapeutic potential of targeting CXCR4 and better understand the associated mechanisms.

Methods: Immunofluorescent staining, confocal microscopy analysis, and immunoblotting were used to analyze CXCR4 expression. BrdU incorporation assays and flow cytometry were used to analyze dynamic …

Effect Of Porosity In Activated Carbon Supports For Silicon-Based Lithium-Ion Batteries (Libs), Yun Jeong Choi, Jeong Bin Choi, Ji Sun Im, Ji Hong Kim

Faculty, Staff and Student Publications

Activated carbon supports for Si deposition with different porosities were prepared, and the effect of porosity on the electrochemical characteristics was investigated. The porosity of the support is a key parameter affecting the Si deposition mechanism and the stability of the electrode. In the Si deposition mechanism, as the porosity of activated carbon increases, the effect of particle size reduction due to the uniform dispersion of Si was confirmed. This implies that the porosity of activated carbon can affect the rate performance. However, excessively high porosity reduced the contact area between Si and activated carbon, resulting in poor electrode stability. …

Genotype-Degree Of Hemolysis Correlation In Hereditary Spherocytosis, Guangxin Peng, Jing Hu, Xiangrong Hu, Baohang Zhang, Kang Zhou, Yang Yang, Youzhen Xiong, Jianping Li, Huihui Fan, Wenrui Yang, Lei Ye, Liping Jing, Li Zhang, Fengkui Zhang

Faculty, Staff and Student Publications

BACKGROUND: Hereditary spherocytosis (HS) is a common inherited hemolytic anemia, caused by mutations in five genes that encode erythrocyte membrane skeleton proteins. The red blood cell (RBC) lifespan could directly reflect the degree of hemolysis. In the present cohort of 23 patients with HS, we performed next-generation sequencing (NGS) and Levitt's carbon monoxide (CO) breath test to investigate the potential genotype-degree of hemolysis correlation.

RESULTS: In the present cohort, we identified 8 ANK1,9 SPTB,5 SLC4A1 and 1 SPTA1 mutations in 23 patients with HS, and the median RBC lifespan was 14(8-48) days. The median RBC lifespan of patients with ANK1, …

Achondroplasia Natural History Study (Clarity): 60-Year Experience In Orthopedic Surgery From Four Skeletal Dysplasia Centers, Nickolas J Nahm, W G Stuart Mackenzie, William G Mackenzie, Ethan Gough, S Shahrukh Hashmi, Janet M Legare, Peggy Modaff, Richard M Pauli, David F Rodriguez-Buritica, Maria Elena Serna, Cory J Smid, Julie Hoover-Fong, Michael B Bober

Faculty, Staff and Student Publications

BACKGROUND: The purpose of this study was to describe the frequency and risk factors for orthopedic surgery in patients with achondroplasia. CLARITY (The Achondroplasia Natural History Study) includes clinical data from achondroplasia patients receiving treatment at four skeletal dysplasia centers in the United States from 1957 to 2018. Data were entered and stored in a Research Electronic Data Capture (REDCap) database.

RESULTS: Information from one thousand three hundred and seventy-four patients with achondroplasia were included in this study. Four hundred and eight (29.7%) patients had at least one orthopedic surgery during their lifetime and 299 (21.8%) patients underwent multiple procedures. …

External Workloads Vary By Position And Game Result In Us-Based Professional Soccer Players, Gary M. Long, Sam M. Joyce, Robert T. Herrington, Kyle B. Fox, Jack E. Mumaugh

International Journal of Exercise Science

International Journal of Exercise Science 16(6): 688-699, 2023. Professional soccer is a physically demanding sport that requires players to be highly trained. Advances using GPS allow the tracking of external workloads for individual players in practice and competition, however, there is a lack of evidence on how these measures impact match results. Therefore, we analyzed external workloads by player position and determined if they vary depending on the result of competitive matches. External workloads were analyzed in professional soccer players (n = 25) across 28 competitive games. One-way ANOVA determined if workloads varied by position (striker – ST, wide …

Efficacy Of A 6-Week Novel Exergaming Intervention Guided By Heart Rate Zones On Aerobic Performance In Children With Fetal Alcohol Spectrum Disorder And Attention-Deficit/Hyperactivity Disorder: A Feasibility Study, Trent Yamamoto, Bilal Pandit, Michael Viggiano, Mitchell S. Mologne, Kristin Daniels, David Gomez, Brett A. Dolezal

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 710-720, 2023. The purpose of this study was to determine the feasibility of a novel exergaming intervention guided by heart rate zones for children and adolescents with fetal alcohol spectrum disorder (FASD) and attention-deficit/hyperactivity disorder (ADHD). Eight study participants (6 females, 2 males, mean age= 11.4±1.4 years old) participated twice weekly over six weeks to complete twelve multimodal exergaming sessions. Participants significantly improved 6MWT from baseline to week 6 (575.4±55.0 m to 732.8±58.9 m; P<0.01), which conferred a 31% improvement in estimated VO_2max (31.5±5.5 ml/kg/min to 40.9±5.9 ml/kg/min), respectively. There was an upward trend of the mean percentage of time spent …

Body Mass Index But Not Physical Activity Level Moderates Lowered Cardiac Baroreflex Sensitivity In People Living With Hiv, Marcus Rangel, Juliana Barros, Deysiane Peres, Pedro Mira, Daniel Martinez, Mateus Laterza, Paulo Farinatti, Juliana Borges

International Journal of Exercise Science

International Journal of Exercise Science 16(2): 700-709, 2023. Reduced cardiac baroreflex sensitivity (cBRS) is an autonomic marker associated with a worse cardiovascular prognosis. Whether cBRS is lowered in people living with HIV (PLHIV) is yet unclear, as well as potential moderator effects of body mass index (BMI) or physical activity (PA) level. The present study aims to compare the spontaneous cBRS in PLHIV vs. HIV-uninfected controls, and to determine among PLHIV the relationship between cBRS vs. body mass index (BMI) and PA level. Total, upward (cBRS+), and downward (cBRS-) cBRS gains were assessed using the sequential method from beat-to-beat …

Collegiate And University Athletic Trainers’ Use Of Squat Based Gradable Movement Screens, Ryan Hipp, Thomas Cappaert, Paula Turocy, Thomas Palmer

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 4: Issue 1, Article 8, 2023. Squat Based Gradable Movement Screens (SBGMS) are orthopedic assessments used to detect and develop rehabilitation plans for patients with poor movement patterns. Reported to limit the effects of injury related factors, SBGMS have become common techniques identified among several professional domains of Athletic Training practice; however, there is limited descriptive data regarding the implementation and extent SBGMS are used by practicing Athletic Trainers (ATs) within the collegiate or university settings. The purpose was to explore the utility of SBGMS among ATs and to investigate the characteristics of …

Hydromethanolic Extract Of Ipomoea Cairica Leaf Extract Ameliorate The Oxidative Activity Of Cadmium Chloride In The Hearts And Testes Of Male Wistar Rats, Omotayo Babatunde Ilesanmi, Toyin Binang, Ridwan Abiodun Lawal

Al-Bahir

Background: Ipomoea cairica (L.) sweet Convolvulaceae also known as morning glory or ‘Railway creeper’ is a traditional herb that has been used in treating various diseases throughout the world. Some of the reported pharmacological activities include antioxidant, anti-inflammatory, and antiviral. Cadmium is a toxic metal that can cause both hearts and testicular damage and its major mechanism is oxidative stress. Objective: Therefore, this study was done to investigate the effect of I. cairica leaf extract (ICE) against cadmium chloride-induced hearts and testicular damage in Wistar rats. Materials and Methods: Twenty male Wistar rats were divided into 4 groups of 5 …

Broad Host Susceptibility Of North American Amphibian Species To Batrachochytrium Salamandrivorans Suggests High Invasion Potential And Biodiversity Risk, Matthew J. Gray, Edward Davis Carter, Jonah Piovia-Scott, J. Patrick W. Cusaac, Anna C. Peterson, Ross D. Whetstone, Andreas Hertz, Aura Y. Muniz-Torres, Molly C. Bletz, Douglas C. Woodhams, John M. Romansic, William B. Sutton, Wesley Sheley, Allan Pessier, Catherine D. Mccusker, Mark Q. Wilber, Debra L. Miller

Agricultural and Environmental Sciences Faculty Research

Batrachochytrium salamandrivorans (Bsal) is a fungal pathogen of amphibians that is emerging in Europe and could be introduced to North America through international trade or other pathways. To evaluate the risk of Bsal invasion to amphibian biodiversity, we performed dose-response experiments on 35 North American species from 10 families, including larvae from five species. We discovered that Bsal caused infection in 74% and mortality in 35% of species tested. Both salamanders and frogs became infected and developed Bsal chytridiomycosis. Based on our host susceptibility results, environmental suitability conditions for Bsal, and geographic ranges of salamanders in the …

Characterizing Effects Of Non-Point Runoff On Subwatersheds Of The Middle White River In Arkansas, Rainee Nicole Lynn Deroin

Student Theses and Dissertations

The Middle White River Watershed located in northeastern Arkansas is part of the larger White River drainage area that flows into the Mississippi River. The watershed has been on the 303(d) list for impairments such as pathogens, turbidity, total suspended solids, and dissolved oxygen. This study monitored water quality and potential toxicity in four subwatersheds of the Middle White River to inform management and regulation downstream conditions. During the three years of weekly sampling from 2019 to 2022, the subwatersheds violated state standards for pathogens, turbidity, and dissolved oxygen. Aquatic and sediment toxicity testing conducted on the four subwatersheds using …

Basic Fibroblast Growth Factor Induces Proliferation And Collagen Production By Fibroblasts Derived From The Bovine Corpus Luteum, Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Cyclic regression of the ovarian corpus luteum, the endocrine gland responsible for progesterone production, involves rapid matrix remodeling. Despite fibroblasts in other systems being known for producing and maintaining extracellular matrix, little is known about fibroblasts in the functional or regressing corpus luteum. Vast transcriptomic changes occur in the regressing corpus luteum, among which are reduced levels of vascular endothelial growth factor A (VEGFA) and increased expression of fibroblast growth factor 2 (FGF2) after 4 and 12 h of induced regression, when progesterone is declining and the microvasculature is destabilizing. We hypothesized that FGF2 activates luteal …

Study On Efficacy And Performance Of Artemisia Argyi Extracts, Zhang Wan-Ping, Zhang Zhi-Yuan, Zhang Dong-Mei, Zhang Qian-Jie, Jiang Wen

Food and Machinery

Objective: By analyzing bacteriostasis and efficacy, the possibility of Artemisia argyi extract to be a plant extract preservative is clarified. It provides a message for the development of natural non-irritating plant extract preservative. Methods: Water and ethanol extracts of A. argyi were prepared. The content of its main active components was determined. Its inhibitory effect and stability on Escherichia coli and Staphylococcus aureus were analyzed. Antioxidant activity, ultraviolet absorption capacity and security were also discussed. Results: The ethanol extract of A. argyi had a significant inhibitory effect on the two kinds of bacteria, which was related to the content of …

Grab Control Method Of Food Sorting Robot Based On 3d Model, Xiao Yao-Xing, Liu Li-Xin, Hu Liu, Lu Yan-Zhi

Food and Machinery

Objective: To solve the problems of poor accuracy and low sorting efficiency of the existing string fruits in robot sorting. Methods: Based on the structure of the high-speed parallel food sorting system, the improved SURF algorithm and the improved RANSAC algorithm were combined to locate the grab points of the food sorting robot, and a three-dimensional grab model was established to realize the automatic and stable grab of string fruits. Taking grapes as an example, the superiority and accuracy of the proposed method were verified by experiments. Results: Compared with the conventional method, the proposed method improved the average capture …

Effects Of Processing On Volatile Components In Different Parts Of The Flowers From Camellia Liberofilamenta, Liu Zhong-Ying, Fang Shi-Mao, Li Qin, Long Hai-Yan, Deng Lun-Xiu

Food and Machinery

Objective: The effect of processing on the volatile fractions of different parts of the flowers of Camellia liberofilamenta was investigated. Methods: Different parts of the whole flowers, petals and stamens of C. liberofilamenta were processed by spreading, microwave killing and drying to make dry samples, separately, and then the effect of processing on volatile components extracted from different parts of the flowers. Results: A total of 73 kinds of volatile substances were identified, which were mainly alcohols, aldehydes, ketones, esters, acids, terpenes and others, and alcohols and aldehydes were the main volatile compounds. In terms of the type and quantity …

Effects Of Sprouting And Hydrothermal Treatment On Active Components And Functional Properties Of Tartary Buckwheat, Luo Jia-Qian, Zhou Li-Ling, Li Gao-Yang, Yuan Hong-Yan, Xie Qiu-Tao

Food and Machinery

Objective: To improve the content of effective components, in vitro antioxidant capacity and α-amylase inhibition rate of tartary buckwheat. Methods: Using Tartary buckwheat as raw material, the effects were studied, including the sprouting and sprouting combined with hydrothermal treatment on the effective components （total flavonoids, total phenols, rutin and quercetin）, the antioxidant activity and hypoglycemia in tartary buckwheat. And the quality of the samples was comprehensively evaluated by principal component analysis. Results: The contents of flavonoids and rutin in germinated group reached the maximum at 6 days after germination, which were 26.54 mg/g and 13.88 mg/g, 1.22 and 2.95 times …

Application Research Advances Of Different Protein Cross-Linking Methods In Cereal Foods Processing, Chen Zhen-Yun, Shen Yu-Xi, Li Jun-Jie, Liu Min-Nan, Wang Li

Food and Machinery

Protein crosslinking plays an important role in cereal food processing. In recent years, many studies have been carried out on the crosslinking of cereal proteins. However, it is not clear about the principles and molecular mechanisms of crosslinking of cereal proteins. In this review, three kinds of different methods, including physical cross-linking, chemical cross-linking and enzymatic cross-linking, for enhancing the crosslinking of cereal proteins in cereal food processing were summarized, and their recent advances were discussed. Moreover, the effects of the three kinds of protein crosslinking methods on the structural properties of cereal proteins and related products were analyzed. Finally, …

Effects Of Egg Yolk/Egg White On The Aggregation Characteristics Of Second-Grade Wheat Flour, Wang Yong-Qing, Wang Jia-Lu, Li Hai-Feng, Fan Pei, Jia Feng

Food and Machinery

Objective: Egg yolk and egg white were added to wheat flour to provide data support for the processing and improvement of characteristic flour food. Methods: The present study aims to disclose the influence of egg yolk and white on the aggregation characteristics of gluten in wheat flour using a special gluten aggregation instrument, as well as second-grade wheat flour and eggs as materials. Moreover, the dough microstructure and starch particle size during the aggregation process were also analyzed. Results: The results showed that BEM dropped 9.5 BU （i.e., 19.39%） with 5 mL egg yolk added. However, the BEM increased by …

Study On Properties Of The Texture And Fermentation Rheology Of Coconut Flour-Wheat Flour Mixed Dough, Li Ming, Sheng Zheng, Yang Ya-Wen, Wu Zi-Jian, Zhang Hai-Hua

Food and Machinery

Objective: To develop fermented baked goods containing coconut powder. Methods: The texture and fermentation rheological properties of coconut flour-wheat flour dough were measured by full texture compression test of TA.XT Plus C physical property tester and F4 Chopin fermentation rheometer. Results: The addition of coconut flour significantly improved dough hardness, chewiness and gumminess. However, the effects on springiness and adhesiveness were different. The dough with 3% coconut flour showed a decrease in dough fermentation performance, such as being harder, delayed fermentation, and small in volume, while the dough with 1% and 5% coconut flour showed a high degree of collapse, …

Effects Of The Levels Of Nacl Added In Soymilk On Texture Characteristics Of White Dried Beancurds, Shu Yuan-Ke, Huang Yi-Qun, Yu Jian, Liu Yong-Le, Li Yan

Food and Machinery

Objective: This research aimed to understand the effects of different levels of NaCl added in soymilk on textural characteristics of resulted white dried beancurds. Methods: The textural characteristics and protein-gel network structures of dried beancurds produced from soymilk added with different levels of NaCl （0～1%） were fully evaluated through texture profile analysis, creep test, scanning electron microscopy and others. Results: The addition of NaCl in soymilk led to a significant increase （P<0.05） of water held in the protein-gel network of dried beancurds as well as a less compact protein-network structure and overall weaker protein-gel. In addition, the levels of NaCl added in soymilk were highly correlated （r was 0.967～0.982） with the yield and water content of the resulted white dried beancurds, but negatively correlated （r was -0.901～-0.994） with water holding capacity, gel strength, cohesiveness, elastic modulus and other textural properties of dried beancurds. Conclusion: The soymilk added with 0.50%～0.75% of NaCl resulted in dried beancurds with required salty taste and acceptable textural characteristics.

Screening And Identification Of Extracellular Polysaccharide-Producing Lactic Acid Bacteria, Chen Jing, Zhou Jia-Hao, Mao Qi-Qi, Tang Xia, Liu Cheng-Guo, Zhou Hui

Food and Machinery

Objective: To screen lactic acid bacteria strains with strong ability to produce extracellular polysaccharide. Methods: Forty strains of lactic acid bacteria isolated and preserved in the laboratory were selected, and the commercial strain L. rhamnosus GG （LGG） was used as the positive control strain. The strains with high extracellular polysaccharide yield were screened by colony drawing method and phenol-sulfuric acid method, and their stress resistance, safety and antibiotic sensitivity were tested in vitro. The phenotypic characteristics and species identification of the finally obtained strains were analyzed. Results: After primary screening and secondary screening of 40 strains of lactic acid bacteria, …

Changes Of Methanol Content During Pectinase Clarification Of Honey Melon Juice, Hou Yan-Xi, Wang Ming-Rui, Li Xin, Jiao Chan

Food and Machinery

Objective: The relationship between the clarification effect and methanol content of honeydew-melon juice was investigated during the optimization of pectinase treatment process. Methods: The effects of pectinase addition, pH value, reaction time, and processing temperature on the clarity and methanol content of honeydew-melon juice were investigated through single factor and orthogonal experiments, by using the transmittance and methanol content as indicators. Results: The order of influencing factors on clarity and methanol content of juice was pH value > pectinase addition > action time > temperature. The optimal conditions for producing honeydew-melon juice with high clarity and high methanol concentration were 0.04 g/100 mL …

Extraction And Composition Analysis Of Flavonoids In Peony Seed Shells, Zou Ping, Zhang Ying-Yang, Zou Lin-Ling

Food and Machinery

Objective: To extract natural flavonoid compounds （MDZK） from peony seed husks for composition analysis and antioxidant research. Methods: The total flavonoids of peony seed shell were extracted by solvent reflux extraction method. The ultraviolet-visible spectrophotometer method was used to determine the MDZK content and antioxidant properties; The total flavonoids extracted were analyzed by LC-MS method. Results: When the solvent was 70% ethanol, the extraction temperature was 70 ℃, the feed-to-liquid ratio was 1∶25 （g/mL）, and the extraction time was 60 min, the MDZK yield was the highest, which was （10.54±0.13）%. When the concentration of MDZK reached 120 μg/mL, the highest …

Purity Determination Of Glutamic Acid By Dsc, Ma Ying-Nan, Li Yu-Pu, Liu Bei-Lei, Ma Yu-Song, Yao Chun-Yi, Hou Lan-Jing

Food and Machinery

Objective: The feasibility of the determination of glutamate purity was studied by differential scanning calorimetry. Methods: Indium and Zinc were used to correct the instrument parameters. Under the conditions of nitrogen atmosphere and flow rate of 50 mL/min, the effects of sample particle size, experimental atmosphere, heating rate and sample quality on the DSC measurement results were investigated, and the glutamic acid purity was determined under the optimal conditions. The determination results were compared between DSC and China Pharmacopeia 2015 titration. Results: Under the condition of weighing 3 mg and heating rate at 40 ℃/min, the purity of glutamic acid …