Life Sciences Commons^™

Alternatives To Antibiotics In Veterinary Medicine: Considerations For The Management Of Johne's Disease, Laura M. O'Connell, Aidan Coffey, Jim O'Mahony

Dept. of Biological Sciences Preprints

Antibiotic resistance has become a major health concern globally, with current predictions expecting deaths related to resistant infections to surpass those of cancer by 2050. Major efforts are being undertaken to develop derivative and novel alternatives to current antibiotic therapies in human medicine. What appears to be lacking however, are similar efforts into researching the application of those alternatives, such as (bacterio)phage therapy, in veterinary contexts. Agriculture is still undoubtedly the most prominent consumer of antibiotics, with up to 70 % of annual antibiotic usage attributed to this sector, despite policies to reduce their use in food animals. This not …

Raw Diffraction Data For 3tel-Hlx-Darpin (Sumo), Wwpdb Id: 7n2b, Supeshala D. Nawarathnage, Parag Laljibhai Gajjar, Derick R. Bunn, Cameron Stewart, Tzanko I. Doukov, James Moody

ScholarsArchive Data

This X-ray diffraction dataset was collected on October 21Oct2020 at SSRL Beamline BL9-2. The crystal was of 3TEL-hlx-DARPin (SUMO) The solved structure used frames 1-1799 and has been deposited in the wwPDB under the PDB ID 7N2B. This data can be readily processed using XDS, AutoProc, CCP4, HKL2000/3000, or other crystallographic X-ray diffraction data processing software. The files have a human-readable header but are otherwise binary.

Pharmacogenetic Testing For The Pediatric Gastroenterologist: Actionable Drug-Gene Pairs To Know., Tracy L. Sandritter, Rachel Chevalier, Rebecca Abt, Valentina Shakhnovich

Manuscripts, Articles, Book Chapters and Other Papers

Gastroenterologists represent some of the earlier adopters of precision medicine through pharmacogenetic testing by embracing upfront genotyping for thiopurine S-methyltransferase nucleotide diphosphatase (TPMT) before prescribing 6-mercaptopurine or azathioprine for the treatment of inflammatory bowel disease. Over the last two decades, pharmacogenetic testing has become more readily available for other genes relevant to drug dose individualization. Common medications prescribed by gastroenterologists for conditions other than inflammatory bowel disease now have actionable guidelines, which can improve medication efficacy and safety; however, a clear understanding of how to interpret the results remains a challenge for many clinicians, precluding wide implementation of …

Novel Potential Targets For Function-Promoting Therapies: Orphan Nuclear Receptors, Anti-Inflammatory Drugs, Troponin Activators, Mas Receptor Agonists, And Urolithin A, Waly Dioh, Vihang Narkar, Anurag Singh, Fady Malik, Luigi Ferrucci, Jean Mariani, Roger A Fielding

Faculty, Staff and Student Publications

In recent years, several new classes of therapies have been investigated with their potential for restoring or improving physical functioning in older adults. These have included Mas receptor agonists, regulators of mitophagy, skeletal muscle troponin activators, anti-inflammatory compounds, and targets of orphan nuclear receptors. The present article summarizes recent developments of the function-promoting effects of these exciting new compounds and shares relevant preclinical and clinical data related to their safety and efficacy. The development of novel compounds in this area is expanding and likely will need the advent of a new treatment paradigm for age-associated mobility loss and disability.

Gata6-As1 Regulates Intestinal Epithelial Mitochondrial Functions, And Its Reduced Expression Is Linked To Intestinal Inflammation And Less Favourable Disease Course In Ulcerative Colitis, Katya E Sosnovski, Tzipi Braun, Amnon Amir, Danielle Moshel, Marina Benshoshan, Nina Levhar, Katia Beider, Rakefet Ben-Yishay, Syed Asad Ali, Sean R Moore, Subra Kugathasan, Ifat Abramovich, Efrat Glick Saar, Batya Weiss, Iris Barshack, Eyal Gottlieb, Tamar Geiger, Shomron Ben-Horin, Igor Ulitsky, Jeffrey S Hyams, Lee A Denson, Yael Haberman

Faculty, Staff and Student Publications

BACKGROUND AND AIMS: Widespread dysregulation of long non-coding RNAs [lncRNAs] including a reduction in GATA6-AS1 was noted in inflammatory bowel disease [IBD]. We previously reported a prominent inhibition of epithelial mitochondrial functions in ulcerative colitis [UC]. However, the connection between reduction of GATA6-AS1 expression and attenuated epithelial mitochondrial functions was not defined.

METHODS: Mucosal transcriptomics was used to conform GATA6-AS1 reduction in several treatment-naïve independent human cohorts [n=673]. RNA pull-down followed by mass spectrometry was used to determine the GATA6-AS1 interactome. Metabolomics and mitochondrial respiration following GATA6-AS1 silencing in Caco-2 cells were used to elaborate on GATA6-AS1 functions.

RESULTS: GATA6-AS1 …

Proteogenomic Landscape Of Gastric Adenocarcinoma Peritoneal Metastases, Shuangtao Zhao, Ruiping Wang, Shumei Song, Dapeng Hao, Guangchun Han, Jianhua Zhang, Namita Shanbhag, Andrew Futreal, Brian Badgwell, Kazuto Harada, George Calin, Jody Vykoukal, Chuan-Yih Yu, Hiroyuki Katayama, Samir M Hanash, Linghua Wang, Jaffer A Ajani

Faculty, Staff and Student Publications

Advanced gastric adenocarcinoma (GAC) often leads to peritoneal carcinomatosis (PC) and is associated with very poor outcome. Here we report the comprehensive proteogenomic study of ascites derived cells from a prospective GAC cohort (n = 26 patients with peritoneal carcinomatosis, PC). A total of 16,449 proteins were detected from whole cell extracts (TCEs). Unsupervised hierarchical clustering resulted in three distinct groups that reflected extent of enrichment in tumor cells. Integrated analysis revealed enriched biological pathways and notably, some druggable targets (cancer-testis antigens, kinases, and receptors) that could be exploited to develop effective therapies and/or tumor stratifications. Systematic comparison of expression …

Loss Of Ampkα2 Promotes Melanoma Tumor Growth And Brain Metastasis, Ping Yuan, Da Teng, Evelyn De Groot, Man Li, Sebastian Trousil, Jason Roszik, Y N Vashisht Gopal, Bin Zheng

Faculty, Staff and Student Publications

AMP-activated protein kinase (AMPK) is a critical cellular energy sensor at the interface of metabolism and cancer. However, the role of AMPK in carcinogenesis remains unclear. Here, through analysis of the TCGA melanoma dataset, we found that PRKAA2 gene that encodes the α2 subunit of AMPK is mutated in ∼9% of cutaneous melanomas, and these mutations tend to co-occur with NF1 mutations. Knockout of AMPKα2 promoted anchorage-independent growth of NF1-mutant melanoma cells, whereas ectopic expression of AMPKα2 inhibited their growth in soft agar assays. Moreover, loss of AMPKα2 accelerated tumor growth of NF1-mutant melanoma and enhanced their brain …

The Effects Of Big Dune And Lava Dune Sand Composition On The Eggs And Larvae Of Pseudocotalpa Giulianii, Cosset Hernandez Pena, Mandy Mountain, Daniel B. Thompson

Spectra Undergraduate Research Journal

Differences in the composition of aeolian sand at Big Dune and sand at Lava Dune that is mixed with basalt and various minerals may affect water retention and the development of insects that are specialized for living in sand environments. These two dunes, located in Nye County, Nevada, are home to the endemic dune scarab beetle, Pseudocotalpa giulianii (PSGU). The differences in sand type may influence the growth patterns of the eggs and larvae of this beetle species that hatches and develops to an adult within the sand. The purpose of this experiment is to test whether sand composition affects …

A Review Of Undrowned: Black Feminist Lessons From Marine Mammals, Nadia G. Dresscher-Lambertus

Feminist Pedagogy

No abstract provided.

Effect Of Plant Population And Phosphorus Fertilizer Application On Dry Matter And Seed Yield Of Two Lablab (Lablab Purpureus) Varieties In Botswana, K. C. Kawonga, F. P. Wandera, S. Mangope, P. Mutshewa, B. M. Lefofe

IGC Proceedings (1977-2023)

Lablab has high potential as a protein source to grazing livestock especially during the dry season in arid Botswana. It produced 8.5t dry matter (DM)/ha and had 14% crude protein with 60% digestibility (APRU, 1988; Aganga, 2003). Lack of agronomic data on lablab production was probably the reason why some farmers in Botswana got yields as low as 300 kg/ha (APRU, 1987). Therefore, a trial of two lablab varieties was conducted to determine the effect of plant population and phosphorus (P) on DM yield.

Forage Yield And Structural Traits Of Tanzaniagrass (Panicum Maximum) At Four Canopy Heights, Carlos A. M. Gomide, Ana C. Ruggieri, R. A. Reis, J. A. Gomide, J. H. De A. Rangel, E. O. Almeida

IGC Proceedings (1977-2023)

Pasture forage production is based on the growth of tillers (Hodgson, 1990). Although the effect of canopy height and structural traits on productivity of temperate grasses are well known e.g. (Bircham & Hodgson,1983; Binnie & Chestnut, 1994) tiller studies on tropical pasture species are scarce.

Forage Yield In Two Tropical Grasses At Different Cutting Intervals And N Levels, Enrique Cortés Díaz, H. Diaz-Solis, A. Saldívar-Fitzmaurice, W. Grant, A. Martínez-Garza, A. Martínez-Hernandez

IGC Proceedings (1977-2023)

Cutting interval and N level determine forage yield in grasses (Whitehead, 1995). Coastcross-1 (Cynodon dactylon and Tifton 68 (Cynodon spp) are tropical grasses of high forage yield potential (Burton, 1972; Burton et al., 1993).

Root Systems In Tropical Pasture Restoration Treatments In Rondônia, Brazil, M. C. Piccolo, K. C. Augusti, C. Neill, L. Fante Junior, M. Bernoux, C. C. Cerri

IGC Proceedings (1977-2023)

Soil management can influence physical and chemical soil properties, with fundamental differences in root system development. Our objectives were to quantify carbon (C) and nitrogen (N) concentrations, dry mass and length of roots subjected to different restoration treatments of degraded pasture.

Economic Methodology For Pasture Grass And Legume Seed Production, D. M. Cino, G. Febles, M. F. Díaz, F. Funes

IGC Proceedings (1977-2023)

The importance and complexity of the industrial process of seed production is known. Thus, conditions should be established for achieving and efficiently controlling the activity in order to know production costs, selling prices and to guarantee economic efficiency. The objective of this paper was to evaluate from an economic point of view, seed production of species of tropical grasses and legumes based on a preliminary methodology facilitating the control of the whole activity.

Integration Of Forage Production In Rice-Based Cropping Systems For Mitigating Forage Crisis Of Ruminant Livestock - Studies In Bangladesh, M. A. Akbar, M. S. U. Bhuiyan, M. S. Islam

IGC Proceedings (1977-2023)

Intensive rice cropping in Bangladesh is causing alarming shortages of forage and low ruminant productivity. This is also causing degeneration of soil fertility. Therefore it is imperative to identify some approach to integrate fodder production into rice cropping systems on rural farms. The integration of legume forage may improve soil fertility and soil structure, thus enhancing crop yield and may provide high quality feed for livestock (Haque 1992). Studies were done to investigate the effects of rice/forage integration on forage yield, soil fertility and also on milk yield of cows fed on the grown forages.

Evaluating The Economic And Environmental Sustainability Of Integrated Farming Systems, C. A. Rotz, M. A. Sanderson, M. Wachendorf, F. Taube

IGC Proceedings (1977-2023)

Economic and environmental sustainability has become a major concern for forage-based animal production in Europe, North America and other parts of the world. Development of more sustainable farming systems requires an assimilation of experimental and modelling research. Field research is critical for supporting the development and evaluation of models, and modelling is needed to integrate farm components for predicting the long-term effects and interactions resulting from farm management changes. Experimentally supported simulation provides a tool for evaluating and comparing farming strategies and predicting their effect on the watershed, region and beyond.

Simulation Of Pasture Phase Options For Mixed Livestock And Cropping Enterprises, L. Salmon, A. D. Moore, J. F. Angus

IGC Proceedings (1977-2023)

In southern Australia, 50% of grain-producing farms also run beef and/or sheep enterprises. Legume pasture leys are used to replace soil nitrogen and manage crop disease risks. Deep-rooted perennials, predominantly lucerne (Medicago sativa), are replacing annual Trifolium subterraneum-based leys to increase pasture production. They also have the environmental benefits of limiting soil acidity, rising water tables and dryland salinity. After recent droughts depletion of soil water by lucerne has penalised wheat yields. Decision support tools can help farmers evaluate the long-term effects of grazed annual and perennial leys on animal and crop production at the whole farm …

Overseeding Cereal Rye And Annual Ryegrass Into Soyabean For Forage As Part Of A Multifunctional Cropping System, L. B. Smith, R. L. Kallenbach

IGC Proceedings (1977-2023)

In the lower Midwest, the longest period of inadequate forage supply from pasture is from mid- December through mid-March (Matches & Burns, 1995). Livestock producers in this region are looking for high quality forage for winter grazing (Kallenbach et al., 2003). Annual ryegrass (Lolium multiflorum Lam.) and cereal rye (Secale cereale L.) are two forages that Missouri's beef producers are interested in to extend the grazing season. The objective of this research was to determine how seeding date impacts the establishment, growth, and forage production of annual ryegrass and cereal rye when planted into soyabean fields …

Previous Grass-Lucerne Mixtures Affect Barley Yield And Quality In A Semiarid Location Of The Canadian Prairie Region, P. G. Jefferson, F. Selles, R. P. Zentner, R. Lemke

IGC Proceedings (1977-2023)

In the semiarid region of the Canadian prairies perennial forages are not rotated with annual crops because previous experiments reported negative impacts (Kilcher and Anderson 1963; Campbell et al. 1990). However, previous research used persistent species while short-lived species could have less adverse effect. Our objective was to compare three grass species in three lucerne mixtures terminated with tillage or herbicide for effects on barley grain, N concentration, and N uptake.

Canavalia Brasiliensis: A Multipurpose Legume For The Sub-Humid Tropics, A. Schmidt, M. Peters, A. Franco, R. Schultze-Kraft

IGC Proceedings (1977-2023)

Canavalia brasiliensis Mart. ex Benth. ("Brazilian jackbean") is a weakly perennial, prostrate to twining herbaceous legume with a wide natural distribution in the New World tropics and subtropics. In comparison with C. ensiformis ("jackbean"), research reports on C. brasiliensis are scattered and restricted to studies done in Latin America. The species develops a dense and extensive, deep-reaching root system and subsequently tolerates a 5-6 month dry period. Based on studies that generally were done with only one genotype, it is adapted to a wide range of soils, including very acid, low-fertility soils. Its main use is as green manure, for …

Relative Forage Yield Of Intercropped Lucerne (Medicago Sativa L.) And Winter Forage Cereals, T. Pereyra, H. Pagliaricci, A. Ohanian

IGC Proceedings (1977-2023)

In tropical regions of the world intercropping is mainly associated with the production of maize for grain, while in temperate areas it is associated with the efficient production of forage. There is increasing interest in the development of cereal - legume intercropping in some temperate regions (reviewed by Mason & Pritchard, 1987). The aim of this study was to compare the relative forage yield of intercropped lucerne and cereal.

The Effectiveness Of Nitrogen Rates On Winter Wheat And White Clover Bi-Cropping Grown For Silage, J. Sowiński

IGC Proceedings (1977-2023)

Whole-crop cereals harvested for silage cover c. 500,000 ha in Europe (Wilkins & Kirilov, 2003). Generally dry matter (DM) yield of small-grain cereals is lower than that of maize. In some investigations, DM yield (of high nutritional value) reached 15 t/ha (Balsdon et al. 1997; Clements et al. 1997). Whole-crop silage produced in a bi-cropping system offers more balanced forage compared to pure cereals and legumes. Nitrogen rates can be decreased with bi-cropping. The aim of this investigation was to compare nitrogen effectiveness using two methods of winter wheat cultivation: direct drilling into stubble and bi-cropping with …

The Influence Of Winter Wheat And White Clover Bi-Cropping System On White Clover Sward Parameters, J. Sowiński

IGC Proceedings (1977-2023)

Whole-crop silage produced in a bi-cropping system represents a low-input forage production system (Clements et al., 1997). Depressing competition of white clover (by mowing or spraying with herbicides) when winter wheat starts its growth is necessary in this system. Winter wheat also competes with white clover during crop growth. The number of growing points as well as the length and weight of stolon are the main parameters that characterise the persistence of white clover (Jorgensen & Ledgard, 1997; Marriott & Haystead 1990). The purpose of the present investigation was to study the effects of a bi-cropping system on some …

Options For Improved Biomass Production In Feeding Systems For Dairying In High Rainfall Environments In New Zealand, John M. De Ruiter, D. R. Wilson, S. Maley, S. M. Henton

IGC Proceedings (1977-2023)

New Zealand dairy production has expanded into marginal climates and soil types on the premise of excellent profitability and efficient utilisation of forage. Annual pasture production in the cool West Coast of the South Island (rainfall 2042-2933 mm) is15,000 kg. Increased farm production and feeding of high quality biomass, from imported feed or supplementary feed crops grown on- farm, are needed to improve milk solid output. Small plot trials with spring and early summer-sown brassicas, cereals and maize were the focus for development of systems to maximise and manage the seasonal feed supply. The effect of sowing time, fertiliser timing …

Implications Of The Use Of Grazing Sheep On Kiwi Fruit Orchard, C. H. E. C. Poli, R. C. Gomes, P. Cinel Filho, M. F. Gomes, A. Zborowki, G. Pires, J. L. Rigon

IGC Proceedings (1977-2023)

In the southern part of Brazil there is an important area of kiwi fruit, mainly cultivated by small farmers. The use of sheep under trees of kiwi fruits could be an interesting alternative for small farmers to reduce their mowing costs, to improve their income and to provide meat for the farmers' family. However there is a lack of information about the damage that the animals could cause to kiwi fruit plants. The objective of this study was to monitor the effect of the use of sheep on a kiwi fruit orchard.

The Effect Of Cutting Regime And Cultivar On Longevity Of Pure Lucerne Stands In Latvia, Z. Gaile

IGC Proceedings (1977-2023)

Lucerne growing is important for high and excellent quality yields of hay or silage without application of N fertilisers, as well as for increasing crop diversity in crop rotation systems. It is comparatively expensive to establish lucerne stands in Latvia. Different aspects can affect longevity of lucerne stands: suitability of cultivar to specific conditions, soil characteristics, different stress conditions and cutting management, including frequency and height of cutting, and critical rest period in autumn (Sheaffer et al., 1988). The aim of our study was to evaluate the persistence of lucerne stands without renovation while obtaining reasonable yields.

The Input Of Forage Legumes In Sustainable Grassland Systems, Žydrė Kadžiulienė, L. Sarunaite

IGC Proceedings (1977-2023)

There is increased interest in sustainable grassland systems. One step towards sustainability is expansion of legume use, because of their potential to fix and transfer nitrogen (N) to subsequent crops. However, legumes can also have negative aspects, such as difficulties in establishment (Porqueddu et al., 2003), lack of persistence, N loss (Scholefield et al., 2002) and accumulation of soil borne disease agents (Kadziulis, 2001). The large variability within legume swards and between years in pastures and leys has encouraged us to search for possibilities to achieve stability of their inputs in sustainable grassland systems.

The Interaction Of Management With Botanical Composition Of Irrigated Grass-Legume Pasture Mixtures In The Intermountain West Usa, J. W. Macadam, T. C. Griggs, G. J. Mileski

IGC Proceedings (1977-2023)

Beef produced on semi-arid range and milk produced in confinement are the main agricultural commodities in the semi-arid western USA. The studies reported here were undertaken to determine the suitability of irrigated pasture as an alternative to traditional beef and dairy production systems. The clipping and grazing studies were not run concurrently or within the same field, but were successive steps in selecting mixtures best-suited for rotational stocking of irrigated pastures in the Intermountain West. Summaries of productivity data have been reported elsewhere (MacAdam, 2002; MacAdam et al., 2004).

Use Of Novel Spatial Presentations Of Plant Species To Improve Legume Abundance, J. M. Sharp, M. J. Jeger, R. W. Fraser, G. R. Edwards

IGC Proceedings (1977-2023)

The benefits of using white clover (Trifolium repens) in pasture grazed by sheep have been widely recognised. However, clover is considered inadequate and risky as the main source of nitrogen input, since its abundance in the pasture is patchy, low (typically less than 20%) and shows great year-to-year variation. This is thought to be due to the costs of nitrogen fixation, competition with grass, the preference for clover by sheep and patchy dung and urine deposition (Schwinning & Parsons, 1996). One possible solution may be the spatial separation of clover from grass, which would remove inter-specific competition, allowing clover to …

Italian Ryegrass And Barley Mixture For Forage Production: Effect Of Harvesting Time On Yield And Quality In Northern Latitudes, Oiva Nissinen, O. Niemeläinen, M. Kontturi

IGC Proceedings (1977-2023)

In northern latitudes forage cereal and Italian ryegrass (IRG) mixtures provide a good source of forage in situations where perennial swards have suffered winter damages (Nissinen, 1994). In this experiment harvesting time of the first cut of an IRG-barley mixture was studied to optimise the yield and quality in the growing season. The objective of the study was to assess if harvesting an IRG-barley mixture later than two weeks after heading, as currently recommended, offers benefits.