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Articles 37951 - 37980 of 256001

Full-Text Articles in Life Sciences

Ebi2, Rodney Till, Dr. Brian Poole Jun 2023

Ebi2, Rodney Till, Dr. Brian Poole

Journal of Undergraduate Research

My project this year was a great learning experience and helped me to better understand the complexity of lab work, as well as, the tools used in troubleshooting problems; including collaboration with lab researchers and professors. I learned about flow cytometry and how to analyze migration data. I also learned how to do transfections with HEK cells and how to separate B cells from a fresh sample of blood. I was thankful that I could spend time doing lab work because it’s the best teaching environment I know of in academia. I came across genuine problems and studied topics with …

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Examination Of The Role Of The Lana Protein In Persistence Of Kaposi’S Sarcoma-Associated Herpesvirus Infections, Marshall Sheide, Dr. Bradford Berges Jun 2023

Examination Of The Role Of The Lana Protein In Persistence Of Kaposi’S Sarcoma-Associated Herpesvirus Infections, Marshall Sheide, Dr. Bradford Berges

Journal of Undergraduate Research

Kaposi’s sarcoma herpes virus (KSHV) is one of seven known cancer causing viruses which have the capacity to persist in the host for many years. This particular virus can undergo a long latent infection which ensures that the virus can hide by not expressing its genes. Last year, we proposed an experiment that would show the importance of a particular gene in the viral genome, LANA, in establishing a latent infection. The experiment required us to create stocks of a mutant virus that did not have the LANA gene inside (bac36deltaLANA) and then infect humanized mice with it. After infection, …

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Search For Mitochondrial Dna Mutation In A Family With Myopathies And Lactic Acidosis, Mary Rennick, Dr. Brent Nielsen Jun 2023

Search For Mitochondrial Dna Mutation In A Family With Myopathies And Lactic Acidosis, Mary Rennick, Dr. Brent Nielsen

Journal of Undergraduate Research

This research focused on the search for the probable mitochondrial DNA (mtDNA) mutation in one family affected with fibromyalgia and lactic acidosis. The hypothesis was that there is mixture of normal and mutated mtDNA, called heteroplasmy. I first obtained the four patients’ consent to participate in the study. The four patients were between the age of 19 and 25 years-­‐two males and two females. They donated urine samples to be used for DNA analysis. I isolated DNA using a Qiagen DNA extraction kit and obtained concentration measurements of 8-­‐ 10 nanograms per microliter using the nanodrop spectrophotometer in the RIC …

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Antibody Production And Diversification In Humanized Mice, Stanton Nielsen, Dr. Brad Berges Jun 2023

Antibody Production And Diversification In Humanized Mice, Stanton Nielsen, Dr. Brad Berges

Journal of Undergraduate Research

Many scientists are skeptical of using humanized mice to study the human adaptive immune response. The reasoning behind this skepticism stems from discrepancies in various studies seen previously. A study conducted by Traggiai et al. showed that humanized mice elicited a human adaptive immune response when challenged with the tetanus toxin (Traggiai). In addition, good antibody responses have been shown in humanized mice when challenged with Dengue virus (Kuruvilla). However, others have found only rare antibody responses to HIV when humanized mice were challenged (Baenziger). As mentioned earlier, a study conducted by Becker et al. showed the production of human …

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Resistance Training And Running Performance And Economy: A Literature Review, James Bottrill Jun 2023

Resistance Training And Running Performance And Economy: A Literature Review, James Bottrill

University Honors Theses

Running performance is largely determined by maximal oxygen uptake (VO2 max), velocity at VO2 max, fractional utilisation of VO2 max and running economy. Running economy is relatively under-researched and could be improved by resistance training because of its neuromuscular underpinnings. However, existing research on this topic is quite unorganised and inconclusive and is yet to prove a direct linkage between resistance training, running economy and performance. This literature review aimed to provide an organised overview of the existing literature, identify and address confounding variables and provide recommendations for future research. A search for studies was conducted via …

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Vitamin D3 As An Adjuvant To Produce Localized Immunity, Kaitlyn Vance, Dr. Eric Wilson Jun 2023

Vitamin D3 As An Adjuvant To Produce Localized Immunity, Kaitlyn Vance, Dr. Eric Wilson

Journal of Undergraduate Research

The aim of our research was to help elucidate the degree of specificity employed by antibody secreting cells (lymphocytes) through a study of the role of CCR10 in the differential homing of these cells to mucosal issues. We used vitamin D3, known to upregulate expression of CCR10 on activated lymphocytes, as an adjuvant in our vaccines and tracked cell homing to mucosal tissues that express the CCR10 ligand CCL28 by detecting antigen specific antibody accumulation. Since mucosal tissues are ideal locations for vaccinations, the long-term aim was to develop a method for targeting antigen specific lymphocytes, and thus robust immune …

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Smokejumper Obituary: Hagen, Eric M. (Mccall 1983), National Smokejumper Association Jun 2023

Smokejumper Obituary: Hagen, Eric M. (Mccall 1983), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

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Animal Communication And Sentience, Catia Correia-Caeiro, Katja Liebal Jun 2023

Animal Communication And Sentience, Catia Correia-Caeiro, Katja Liebal

Animal Sentience

Segundo-Ortin & Calvo (S&C) argue for sentience in plants on the basis of several studies of what they describe as "cognitive abilities" in plants. As other commentaries (e.g., Brooks Pribac, 2023; Damasio & Damasio, 2023; ten Cate, 2023) have pointed out, however, there is some misuse of several concepts, and a lack of evidence for sentience. We try to clarify three questions in S&C’s discussion: (1) How is communication defined and conceptualised in animal research? (2) Is plant communication comparable to animal communication? (3) Is communication (or the process we see in plants) a good basis for inferring sentience in …

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Evidence-Based Guide To Using Artificial Introns For Tissue-Specific Knockout In Mice, Elena Mcbeath, Keigi Fujiwara, Marie-Claude Hofmann Jun 2023

Evidence-Based Guide To Using Artificial Introns For Tissue-Specific Knockout In Mice, Elena Mcbeath, Keigi Fujiwara, Marie-Claude Hofmann

Faculty, Staff and Student Publications

Up until recently, methods for generating floxed mice either conventionally or by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 (CRISPR-associated protein 9) editing have been technically challenging, expensive and error-prone, or time-consuming. To circumvent these issues, several labs have started successfully using a small artificial intron to conditionally knockout (KO) a gene of interest in mice. However, many other labs are having difficulty getting the technique to work. The key problem appears to be either a failure in achieving correct splicing after the introduction of the artificial intron into the gene or, just as crucial, insufficient functional KO of the …

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The Effect Of Harvesting Strategy Of Grass Silage On Milk Production, K. Kuoppala, M. Rinne, J. Nousiainen, P. Huhtanen Jun 2023

The Effect Of Harvesting Strategy Of Grass Silage On Milk Production, K. Kuoppala, M. Rinne, J. Nousiainen, P. Huhtanen

IGC Proceedings (1977-2023)

Timing of harvest in primary growth of grass is a major factor affecting D-value (digestible organic matter, g/kg DM) of silage and dry matter (DM) consumption and milk production of dairy cows (Rinne, 2000). The objective of this research was to investigate whether there is a similar pattern in regrowths of grass.

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The Effect Of Harvesting Strategy Of Grass Silage On Milk Production, K. Kuoppala, M. Rinne, J. Nousiainen, P. Huhtanen Jun 2023

The Effect Of Harvesting Strategy Of Grass Silage On Milk Production, K. Kuoppala, M. Rinne, J. Nousiainen, P. Huhtanen

IGC Proceedings (1977-2023)

Timing of harvest in primary growth of grass is a major factor affecting D-value (digestible organic matter, g/kg DM) of silage and dry matter (DM) consumption and milk production of dairy cows (Rinne, 2000). The objective of this research was to investigate whether there is a similar pattern in regrowths of grass.

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The Effects Of Offering Hay, Pit-Stored Grass Silage Or Big-Bale Silage To Pregnant Ewes On Ewe And Lamb Performance, T. F. Crosby, P. J. Quinn, J. J. Callan, T. Mcgrane Jun 2023

The Effects Of Offering Hay, Pit-Stored Grass Silage Or Big-Bale Silage To Pregnant Ewes On Ewe And Lamb Performance, T. F. Crosby, P. J. Quinn, J. J. Callan, T. Mcgrane

IGC Proceedings (1977-2023)

Hay and pit silage have been used extensively for feeding sheep over the winter period, but in recent years, especially on smaller farms, big-bale silage has become increasingly popular. However, there is limited comparative information on the use of big-bale silage for sheep, especially in relation to the effects of chopping and growth stage at harvest. This study compared the performance of ewes offered hay, pit silage, or big-bale grass silage made from either chopped or unchopped grass and cut from the same field at the same time.

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The Effects Of Offering Grass Or Maize Silages To In-Lamb Ewes On Body Weight And Condition Changes, Colostrum Yield And Quality, T. F. Crosby, P. J. Quinn, J. J. Callan, P. Reilly, B. Flynn, D. Cunningham, T. Massey Jun 2023

The Effects Of Offering Grass Or Maize Silages To In-Lamb Ewes On Body Weight And Condition Changes, Colostrum Yield And Quality, T. F. Crosby, P. J. Quinn, J. J. Callan, P. Reilly, B. Flynn, D. Cunningham, T. Massey

IGC Proceedings (1977-2023)

Hay and more recently grass silage (GS) have been the traditional feeds for sheep in Ireland over the winter period. Alternatives such as maize silage (MS) are becoming increasingly important as winter forage sources especially for cattle. This study sought to evaluate grass silage and maize silage when offered to pregnant ewes.

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Round-Bale Silage Preparation Of Rice Straw, Yimin Cai, C. Xu, N. Yoshida, M. Ogawa Jun 2023

Round-Bale Silage Preparation Of Rice Straw, Yimin Cai, C. Xu, N. Yoshida, M. Ogawa

IGC Proceedings (1977-2023)

Rice straw is an important feed resource for ruminants. In Japan, rice straw cannot be fully dried due to the usually humid autumn season, which leads to about 70% of the production being ploughed back or incinerated. Therefore, the development of techniques to enhance the long-term preservation and quality of rice straw is of great importance. In this work, a new lactic acid bacterium was used as a silage inoculant, and its effect on round-bale silage preparation from fresh rice straw was examined.

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Factors Affecting Bag Silo Densities And Losses, R. E. Muck, B. J. Holmes Jun 2023

Factors Affecting Bag Silo Densities And Losses, R. E. Muck, B. J. Holmes

IGC Proceedings (1977-2023)

Bag silos (polyethylene tubes, 30 to 90 m length, 2.4 to 3.7 m diameter, 0.22 mm thick) are used on approximately one-third of the dairy farms in the U.S.A. for making silage, and the level of adoption is increasing rapidly. Unfortunately, almost no research data have been published on these types of silos. Our objective was to measure densities and losses in bag silos at three farms, looking for causes of variation in both.

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Moisture Control, Inoculant And Particle Size In Tropical Grass Silages, S. F. Paziani, L. G. Nussio, D. R. S. Loures, L. J. Mari, José Leonardo Ribeiro, P. Schmidt, M. Zopollatto, M. C. Junqueira, A. F. Pedroso Jun 2023

Moisture Control, Inoculant And Particle Size In Tropical Grass Silages, S. F. Paziani, L. G. Nussio, D. R. S. Loures, L. J. Mari, José Leonardo Ribeiro, P. Schmidt, M. Zopollatto, M. C. Junqueira, A. F. Pedroso

IGC Proceedings (1977-2023)

Decreased fermentation and spoilage losses with improved aerobic stability during feed out can be accomplished by several strategies, such as wilting, addition of microbial additives and moisture absorbents. Particle size reduction may increase bulk density and improve the fermentation. The objective of this trial was to evaluate the effects of particle size, moisture content and a microbial additive on chemical-physical parameters and losses in silages made from Tanzania grass.

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Effect Of A New Microbial Strain As An Inoculant On The Quality Of Maize Silage, J. G. Kim, J. S. Ham, E. S. Chung, S. Seo, J. K. Lee Jun 2023

Effect Of A New Microbial Strain As An Inoculant On The Quality Of Maize Silage, J. G. Kim, J. S. Ham, E. S. Chung, S. Seo, J. K. Lee

IGC Proceedings (1977-2023)

Lactic acid bacteria play a key role in making silage from forage, and lactic acid bacteria selected from good silage could be expected to be suitable inocula for making good silage. Thus, the purpose of this study was to examine the such novel lactic acid bacteria for making high quality maize silage.

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The Potential Of Different Forage Combinations For Green-Chop Silage, Trevor L. Knight, Tom J. Fraser, T. A. White, M. G. Hyslop Jun 2023

The Potential Of Different Forage Combinations For Green-Chop Silage, Trevor L. Knight, Tom J. Fraser, T. A. White, M. G. Hyslop

IGC Proceedings (1977-2023)

On the Canterbury Plains of New Zealand (NZ) there is an opportunity on cropping farms, between summer harvest and autumn/winter sowing, to grow forage crops to make high quality silage. Recently, cereal cultivars have been specifically bred for forage production and suitability for whole-crop silage (de Ruiter et al. 2002), and also high legume (e.g. sulla) content forage mixes have resulted in high quality silages with high lactic acid and soluble carbohydrate content (Niezen et al. 1998). This trial aimed to determine the yield potential of various cereal/legume forage mixtures summer sown and harvested for silage in autumn.

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A New System For The Evaluation Of The Fermentation Quality Of Silages, E. Kaiser, K. Weiß Jun 2023

A New System For The Evaluation Of The Fermentation Quality Of Silages, E. Kaiser, K. Weiß

IGC Proceedings (1977-2023)

Depending on the content of nitrate in green forage, the pattern of fermentation products in silages differ significantly (Weiß & Kaiser, 2001). The systems, which are now common in practice for evaluating the quality of silage fermentation, characterise fermentation quality incorrectly because the evaluation is influenced by the chemical composition of green forage. The aim of this work was to derive an evaluation system for fermentation quality, which is independent from the chemical composition of green forage.

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Calculation Of Forage Value And Suitability For Silage Of Autochthonous Plant Mixtures Found In Peat Soil Grassland In Relation To The Cut-Off Date, Lisa Dittmann, Renate Bockholt Jun 2023

Calculation Of Forage Value And Suitability For Silage Of Autochthonous Plant Mixtures Found In Peat Soil Grassland In Relation To The Cut-Off Date, Lisa Dittmann, Renate Bockholt

IGC Proceedings (1977-2023)

The semi-extensive management of permanent grassland results in autochthonous plant mixtures whose productivity and forage quality dynamics are relatively unknown, but important for its utilisation. There are enormous differences in forage value and the suitability for silage depending on botanical composition, cutting date or grazing date. The time-based changes in the forage value are of economic interest for the farmers and important for determining a utilisation strategy.

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Influence Of Grass Species And Sample Preparation On Ensiling Characteristics, D. J. R. Cherney, M. A. Alessi, J. H. Cherney Jun 2023

Influence Of Grass Species And Sample Preparation On Ensiling Characteristics, D. J. R. Cherney, M. A. Alessi, J. H. Cherney

IGC Proceedings (1977-2023)

Laboratory silos are considered a practical method of comparing a number of treatments (O'Kiely, 1993). Cherney et al. (2004) reported that vacuum-sealed polyethylene bags effectively ensiled corn silage samples in the laboratory. Grasses, with their inherently higher buffering capacities and lower sugar levels, generally are more difficult to ensile. Objectives were to evaluate the influence of species and chopping (whole vs. shredded) on pH and volatile fatty acid profile of grasses ensiled in vacuum-sealed polyethylene bags and to assess the suitability of this method as a laboratory ensiling method.

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Ensiling Characteristics And Ruminal Degradation Of Italian Ryegrass With Or Without Wilting And Added Cell Wall Degrading Enzymes, Z. Yu, Jianguo Han, Z. He, X. Qingfang, B. Chunsheng, N. Nishino Jun 2023

Ensiling Characteristics And Ruminal Degradation Of Italian Ryegrass With Or Without Wilting And Added Cell Wall Degrading Enzymes, Z. Yu, Jianguo Han, Z. He, X. Qingfang, B. Chunsheng, N. Nishino

IGC Proceedings (1977-2023)

The previous experiment (Yu zhu et al.,1999) has shown that the efficacy of added enzymes varied greatly according to the DM content of the material crop. The silage DM did not alter the effects of enzymes on the in vitro digestion of NDF (Yu zhu et al.,1999, Yu zhu et al.,2000). The aim of this experiment was to study the effect of wilting and enzymes on fermentation quality, chemical composition and in situ digestion of Italian ryegrass (Lolium multiflorum Lam.) silage.

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Ensilability And Silage Quality Of Different Festulolium Hybrids In Comparison To Festuca Arundinacea, K. Banzhaf, Wilhelm Opitz V. Boberfeld Jun 2023

Ensilability And Silage Quality Of Different Festulolium Hybrids In Comparison To Festuca Arundinacea, K. Banzhaf, Wilhelm Opitz V. Boberfeld

IGC Proceedings (1977-2023)

Festulolium hybrids as cool-season grasses may be used as dominant species for winter pastures in year-round outdoor livestock systems. The utilisation of these species during summer is limited due to low intake as a fresh pasture grass by grazing ruminants. Therefore, ensiling the primary growths of these hybrids may be an alternative approach to using these species during the growing season. However, information on the quality of Festulolium silages under central European conditions is not available. The objective of this research was to determine ensilability and silage quality of four Festulolium cultivars (of festucoid or loloid type) compared to one …

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Effect Of Agronomic Management On Feeding Value Of Festulolium Hybrids For Winter Pasture, Wilhelm Opitz V. Boberfeld, K. Banzhaf Jun 2023

Effect Of Agronomic Management On Feeding Value Of Festulolium Hybrids For Winter Pasture, Wilhelm Opitz V. Boberfeld, K. Banzhaf

IGC Proceedings (1977-2023)

Festulolium spp. are considered to combine the distinctive winterhardiness of Festuca species with the high forage quality of Lolium species. Such cultivars may be particularly appropriate for winter pastures, but knowledge about quality aspects during winter under central European conditions is not available. The objective of this research was to determine forage quality of four Festulolium cultivars (festucoid type or loloid type) during winter under varying agronomic treatments.

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Nutrient Composition Of Napier Grass (Pennisetum Purpureum) And Napier Grass Silages Made With Different Additives, A. A. Aganga, U. J. Omphile, J. C. Baitshotlhi Jun 2023

Nutrient Composition Of Napier Grass (Pennisetum Purpureum) And Napier Grass Silages Made With Different Additives, A. A. Aganga, U. J. Omphile, J. C. Baitshotlhi

IGC Proceedings (1977-2023)

Forage contributes about 73% and 95% of the diets of ruminants fattened on grain supplements and on natural ranges respectively (Sarwar & Nisa, 1998). Napier grass is one of the highest yielding tropical forage grasses and was shown to provide a good quality silage when it was supplemented with molasses, as the fermentation quality was not affected by the high storage temperature (40oC). The objectives of this study were to determine the chemical composition of silages when ensiled alone or mixed with additives.

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A Modified Forage System For Stocker Production In The Southern Great Plains, Usa, B. K. Northup, H. S. Mayeux, W. A. Phillips Jun 2023

A Modified Forage System For Stocker Production In The Southern Great Plains, Usa, B. K. Northup, H. S. Mayeux, W. A. Phillips

IGC Proceedings (1977-2023)

Putting low-cost gain on yearling cattle with forages is an important agricultural activity in the Southern Great Plains. The primary forage system within the area incorporates two forages: winter wheat (Triticum aestivum) for grazing in fall through spring, and warm- season grasses in the summer (Fig. 1). These systems have significant gaps in time when high-quality forage is not available. This study tested the function of introduced cool-season perennial grasses in filling the spring gap, and their capacity as large-scale replacements for winter wheat.

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Graze-Out Plus: Filling Forage Gaps In The Southern Great Plains, Usa, B. K. Northup, W. A. Phillips, H. S. Mayeux Jun 2023

Graze-Out Plus: Filling Forage Gaps In The Southern Great Plains, Usa, B. K. Northup, W. A. Phillips, H. S. Mayeux

IGC Proceedings (1977-2023)

Putting low-cost gain on yearling cattle with forages is a significant agricultural activity in the Southern Great Plains. The primary forage system within the area has two components: winter wheat (Triticum aesitivum) grazed from fall through spring (Redmon et al., 1995), and warm-season perennial grasses for summer grazing. This system has significant gaps (Fig. 1) when high-quality forage is not readily available (September-November and May-June). Introduced cool-season perennial grasses have longer growing seasons than wheat, and could help fill these gaps. This experiment tested the function of an introduced cool-season perennial grass, new to the southern Great Plains, …

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An Evaluation Of Grazing Value Of Maize And Companion Crops For Wintering Lactating Ewes, E. A. Van Zyl, C. S. Dannhauser Jun 2023

An Evaluation Of Grazing Value Of Maize And Companion Crops For Wintering Lactating Ewes, E. A. Van Zyl, C. S. Dannhauser

IGC Proceedings (1977-2023)

Northwestern KwaZulu-Natal (KZN), in South Africa, is well known for its sheep production from natural rangeland in summer (October to May). During winter, however, the nutritional value of the rangeland cannot maintain young growing sheep or pregnant and lactating ewes. With this in mind, Lyle (1991) suggested the use of planted pastures for the winter. Crichton et al (1998) and Esterhuizen & Niemand (1989) suggested the use of maize crop residues for both cattle and sheep during winter, whereas Moore (1997) evaluated grazing (not harvested) maize for this purpose. He found that the crude protein content of the crop was …

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An Agronomic Evaluation Of Grazing Maize Combined With Companion Crops For Sheep In Northwestern Kwazulu-Natal, South Africa, C. S. Dannhauser, E. A. Van Zyl Jun 2023

An Agronomic Evaluation Of Grazing Maize Combined With Companion Crops For Sheep In Northwestern Kwazulu-Natal, South Africa, C. S. Dannhauser, E. A. Van Zyl

IGC Proceedings (1977-2023)

Northwestern KwaZulu-Natal (KZN), in South Africa, is well known for its sheep production from natural rangeland in summer (October to May). During winter however, the nutritional value of the rangeland cannot maintain young growing sheep or pregnant and lactating ewes. With this in mind Lyle (1991) suggested the use of planted pastures for the winter. Crichton, Gertenbach & Henning (1998) and Esterhuizen & Niemand (1989) suggested maize crop residues for both cattle and sheep during winter, whereas Moore (1997) evaluated grazing maize (not harvested) for this purpose. He found that the protein content of the crop was inadequate and for …

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The Use Of Forage Supplements To Overcome Seasonal Shortages Of Grazed Herbage In Dairy Production Systems, A. Hameleers, D. J. Roberts Jun 2023

The Use Of Forage Supplements To Overcome Seasonal Shortages Of Grazed Herbage In Dairy Production Systems, A. Hameleers, D. J. Roberts

IGC Proceedings (1977-2023)

In most dairy production systems, grazed herbage is potentially the cheapest forage resource. However, while the availability is affected by seasonal changes in herbage growth, and/or between year variations in climatic conditions, the requirements of dairy production systems tend to remain constant throughout the season. This paper summarises five experiments that examined the effect of the characteristics of the forage supplement (dry matter, DM; metabolisable energy, ME, content; Type) and the effect of stage of lactation and access method to the supplement on animal performance.

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