Life Sciences Commons^™

Optimising Forage Production On Degraded Lands In The Dry Tropics Through Silvopastoral Systems, P. S. Pathak

IGC Proceedings (1977-2023)

In India, 187 M ha out of a total area of 328 M ha face the problem of land degradation, mostly due to water and wind erosion. The problems are aggravated by poor land cover and increasing pressure of human and livestock populations. There is over-exploitation of the scarce resources of forage and firewood. Several techniques, including watershed based silvopastoral land use have been proposed (Patil & Pathak, 1977). Tree, grass and legume based systems have been tried after land treatment to reduce runoff and soil loss while meeting the forage needs of the livestock and firewood for cooking in …

The Valuation Of Service Of Recreation Function Of Dalinor National Natural Reserve, G. Qiao, H. Wang, G. Han, M. Zhao

IGC Proceedings (1977-2023)

Dalinor National Natural Reserve is located in the west of Keshketeng Banner of Chifeng City, Inner Mongolia Autonomous Region (IMAR). It covers a total area of 119,413.35 ha, and is in drought or semi-drought continental climatic zone. DNNR is a comprehensive natural reserve made up of different ecosystems including lakes, prairies, wetlands, and forests. Dalinor Lake in DNNR is regarded as the paradise of birds, covering an area of 238 km² between sandy lands and grasslands. DNNR was established in 1987 as a provincial natural reserve and was upgraded to a National Natural Reserve by the State Council of …

Significance Of Grasslands In Protected Forest Areas, C. M. Mishra, A. Kumar, B. C. Tiwari, S. L. Singh

IGC Proceedings (1977-2023)

This paper describes case studies of grassland formation in the protected forest areas of the Indian state of Uttar Pradesh. This state has a total forest/tree cover of 8.84%, whereas the protected forest areas comprise only 2.54%.The protected areas constitute, one national park, eleven wildlife sanctuaries and thirteen bird sanctuaries.

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Profiling And Verifying The Substrates Of E3 Ubiquitin Ligase Rsp5 In Yeast Cells, Shuai Fang, Geng Chen, Yiyang Wang, Rakhee Ganti, Tatiana A Chernova, Li Zhou, Savannah E Jacobs, Duc Duong, Hiroaki Kiyokawa, Yury O Chernoff, Ming Li, Natalia Shcherbik, Bo Zhao, Jun Yin

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Yeast is an essential model organism for studying protein ubiquitination pathways; however, identifying the direct substrates of E3 in the cell presents a challenge. Here, we present a protocol for using the orthogonal ubiquitin transfer (OUT) cascade to profile the substrate specificity of yeast E3 Rsp5. We describe steps for OUT profiling, proteomics analysis, in vitro and in cell ubiquitination, and stability assay. The protocol can be adapted for identifying and verifying the ubiquitination targets of other E3s in yeast. For complete details on the use and execution of this protocol, please refer to Wang et al.

Assessing Determinants Of Participation In Conservation Programs In The U.S., Manita Ale, Simanti Banerjee, Taro Mieno, Karina A. Schoengold

Cornhusker Economics

In order to generate ecosystem services (ES) from privately owned intensively managed agricultural land, the United States Department of Agriculture (USDA)’s Farm Service Agency (FSA) and Natural Resources Conservation Service (NRCS) have implemented many conservation programs such as the Conservation Reserve Program (CRP), Conservation Stewardship Program (CSP), and Environmental Quality Incentives Program (EQIP), to name a few. These programs provide financial and technical assistance to farmers and ranchers to adopt land management practices that generate ES.

Examining The Restoration Potential Of Prescribed Fire And Tree Girdling Treatments On A Fire Dependent Ecosystem: Ecosystem Responses And Management Implications, Rebecca Daigle

Masters Theses

Oak savannas have greatly declined since the 1800s and management is needed to maintain these rare community types. A combination of prescribed burns and mechanical thinning are a common practice to increase forb abundance and biodiversity. This study examines the viability of another technique to thin the canopy called girdling, which was used in conjunction with low-intensity prescribed burns. Early summer prescribed burns were conducted in 2017, 2019, and 2021, while girdling treatments were implemented in 2017 and 2019. The study was conducted in Kent County, Michigan at two degraded former oak savanna communities. In 2022, we collected data to …

Investigating Transcriptional Anomalies During Persistence In Chlamydia Trachomatis, Nathan D. Hatch

Theses & Dissertations

Chlamydia trachomatis (Ctr) is the leading cause of bacterial sexually transmitted infections. Ctr, a Gram-negative, obligate intracellular bacterium, develops within a membrane-bound vacuole termed an inclusion. This protective, nutrient rich, intracellular environment has allowed Ctr to undergo extensive reductive evolution – resulting in a streamlined genome of approximately 1 Mbp and the loss or fragmentation of several biosynthetic pathways, including the ability to synthesize tryptophan (Trp) de novo. During infection, the host immune system releases the cytokine IFNγ. Upon binding to a host cell, IFNγ stimulates the expression of indoleamine-2,3-dioxygenase, an enzyme capable of catabolizing cytosolic Trp, thereby starving …

Cytokine Profiles In Peruvian Pinnipeds And Their Relationships With Environmental Stressors, Mykenzee L. Munaco

All HCAS Student Capstones, Theses, and Dissertations

Large colonies of two vulnerable, sympatric pinniped species, Peruvian fur seals (Arctocephalus australis Peruvian subpopulation; PFS) and South American sea lions (Otaria byronia; SASL), reside and reproduce in Punta San Juan, Peru (PSJ) where they are exposed to stressors such as environmental contaminants and El Niño-Southern Oscillation (ENSO) events. Cytokine concentrations can be indicative of an animal’s health and immune function by allowing for immunoresponse characterization as Th1 or Th2 dominant. Utilizing an archived pinniped plasma collection of samples from 2009-2019 (n=148), 13 cytokines were analyzed in PFS and SASL: GM-CSF, IFN-γ, KC-like, MCP-1, TNF-α, IL-2, IL-6, …

Evaluating Post-Release Survival And Distribution Of Juvenile Swordfish (Xiphias Gladius) Caught On Buoy Gear Within The Gulf Of Mexico And The Florida Straits, Cody Rewis

All HCAS Student Capstones, Theses, and Dissertations

Buoy gear used to target swordfish (Xiphias gladius) has become an increasingly popular commercial gear type throughout the United States fisheries in both the Atlantic and Pacific oceans. Buoy gear can be defined as a series of independent free-floating gear where each rig consists of at least one floatation device, a vertical mainline, and no more than two hooks. Because of the potential modifications to the component parts and deployment strategies, buoy gear has been hypothesized to decrease bycatch interaction and dead discard numbers by targeting specific depths of swordfish habitat throughout various times of the day. Under the Deepwater …

A Review Of Endoparasites Found In Order Pelecaniformes And Order Ciconiiformes Birds Of Southeast Florida, Kaya C. Wells

All HCAS Student Capstones, Theses, and Dissertations

The focus of this research was to investigate the parasite diversity for common wading birds from four families, Pelecanidae, Ardeidae, Threskiornithidae and Ciconiidae found in southeast Florida which includes Broward, Monroe, Miami-Dade and Palm Beach counties. This study also determined gaps in the literature for the selected species. A total of 116 papers were used to create host-parasite and parasite-host checklists for the nineteen wading bird species selected from the four families, twenty-eight of these studies occurred within the state of Florida. Statistical analyses demonstrated strong correlations between the number of papers found for each species and the number of …

Unlocking Insights Into Crop Growth And Nutrient Distribution: A Geospatial Analysis Approach Using Satellite Imagery And Soil Data, Olatunde D. Akanbi, Brian Gonzalez Hernandez, Erika I. Barcelos, Arafath Nihar, Laura S. Bruckman, Yinghui Wu, Jeffrey Yarus, Roger H. French

Student Scholarship

Accurate monitoring of crop growth and nutrient distribution is crucial for optimizing agricultural practices, promoting a sustainable environment, and ensuring long-term food production. In this study, we propose a novel and comprehensive approach to monitor crop growth and nutrient distribution in large-scale agricultural landscapes. Our methodology combines advanced geospatial and temporal analysis techniques, providing valuable insights into the intricate relationships between crop health, soil nutrients, and other essential soil properties.

To monitor vegetation dynamics, we obtained data from the IBM EIS (Environment Intelligence Suite) and processed it using our HPC (High-Performance Computing) infrastructure. This is ingested into our CRADLE (Common …

Ten Tips Taylored For Successful Talks With Landowners In Ranching And Range Management Landscapes, Taylor J. Linder, Susan N. Ellis-Felege, Christopher J. Felege

Biology Faculty Publications

Privately owned lands offer substantial land area that have the potential to contribute greatly to wildlife conservation. However, it is dubious for private landowners to engage in such programs that have not readily accepted their input. Given the future directions of wildlife conservation and needing to heavily integrate private lands into habitat objectives to meet wildlife population standards, it will be of growing importance to consider the needs and wants of private landowners to meet production goals and simultaneously wildlife habitat goals (and not the other way around). We recognize the important methodological research that has built foundations for current …

A Quantification Of The Metabolic Demand Wrought By Explosive Ordinance Disposal Load Carriage, Gage Cousineau

Online Theses and Dissertations

In the last 7 years, there have been landmark studies concerning Explosive Ordinance Disposal (EOD) load carriage. These studies (Bach et al., 2017; Wu et al., 2022) have worked to quantify the ability of an operator to work while encumbered by EOD personal protective equipment (PPE), but have not evaluated possible sources of mitigation for the increased demand. Intuitively, it seems that greater levels of fitness would modify the degree of increased demand an operator may acquire during EOD load carriage. The current study seeks to illuminate relationships between strength, power, and/or endurance and EOD load carriage performance. Recreationally trained …

Effects Of Plyometric- And Cycle-Based High-Intensity Interval Training On Body Composition, Aerobic Capacity, And Muscle Function In Young Females: A Field-Based Group Fitness Assessment, A. J. Holmes, M. T. Stratton, A. R. Bailly, J. S. Gottschall, Y. Feito, P. L. Ha, A. Lavigne, K. Persaud, H. L. Gagnon, A. Krueger, A. Modjeski, T. A. Esmat, L. N. Harper, T. A. Vandusseldorp, Garrett Hester

Faculty Articles

High-intensity interval training (HIIT) is an effective alternative to moderate intensity continuous training for improvements in body composition and aerobic capacity; however, there is little work comparing different modalities of HIIT. The purpose of this study was to compare the effects of plyometric- (PLYO) and cycle-oriented (CYC) HIIT on body composition, aerobic capacity, and skeletal muscle size, quality, and function in recreationally trained females. Young (21.7 ± 3.1 yrs), recreationally active females were quasi-randomized (1:1 ratio) to 8 weeks of twice weekly PLYO (n = 15) or CYC (n = 15) HIIT. Body composition (four-compartment model), VO_{2peak …}

Real-World Experience Of Patients With Multiple Myeloma Receiving Ide-Cel After A Prior Bcma-Targeted Therapy, Christopher J Ferreri, Michelle A T Hildebrandt, Hamza Hashmi, Leyla O Shune, Joseph P Mcguirk, Douglas W Sborov, Charlotte B Wagner, M Hakan Kocoglu, Aaron Rapoport, Shebli Atrash, Peter M Voorhees, Jack Khouri, Danai Dima, Aimaz Afrough, Gurbakhash Kaur, Larry D Anderson, Gary Simmons, James A Davis, Nilesh Kalariya, Lauren C Peres, Yi Lin, Murali Janakiram, Omar Nadeem, Melissa Alsina, Frederick L Locke, Surbhi Sidana, Doris K Hansen, Krina K Patel, Omar Alexis Castaneda Puglianini

Faculty, Staff and Student Publications

Most patients with multiple myeloma experience disease relapse after treatment with a B-cell maturation antigen-targeted therapy (BCMA-TT), and data describing outcomes for patients treated with sequential BCMA-TT are limited. We analyzed clinical outcomes for patients infused with standard-of-care idecabtagene vicleucel, an anti-BCMA chimeric antigen receptor (CAR) T-cell therapy, at 11 US medical centers. A total of 50 patients with prior BCMA-TT exposure (38 antibody-drug conjugate, 7 bispecific, 5 CAR T) and 153 patients with no prior BCMA-TT were infused with ide-cel, with a median follow-up duration of 4.5 and 6.0 months, respectively. Safety outcomes between cohorts were comparable. The prior …

Insights On The Distribution Of Struthanthus Palmeri Kuijt (Loranthaceae), The Northernmost Species Of The Genus, Mónica I. Miguel-Vázquez, Yasser S. López De Olmos-Reyes, Maria Guadalupe Maldonado-Borja, Rosa Cerros-Tlatilpa

Western North American Naturalist

Struthanthus (Loranthaceae) is native to the Americas, and several species occur in Mexico, some of which are endemic, such as S. palmeri. Within the genus, S. palmeri has the northernmost distribution, inhabiting the arid environments of northwestern Mexico near the border with the United States. In this study, the known distribution of the species was updated. The elevation, hosts, and vegetation types where S. palmeri occurs were compiled to infer its potential distribution, using ecological niche modeling. Based on the results, the endemic status of S. palmeri is reinforced. This taxon occurs in several types of vegetation, from near …

Genetic Control Of Mrna Splicing As A Potential Mechanism For Incomplete Penetrance Of Rare Coding Variants, Jonah Einson, Dafni Glinos, Eric Boerwinkle, Peter Castaldi, Dawood Darbar, Mariza De Andrade, Patrick Ellinor, Myriam Fornage, Stacey Gabriel, Soren Germer, Richard Gibbs, Craig P Hersh, Jill Johnsen, Robert Kaplan, Barbara A Konkle, Charles Kooperberg, Rami Nassir, Ruth J F Loos, Deborah A Meyers, Braxton D Mitchell, Bruce Psaty, Ramachandran S Vasan, Stephen S Rich, Michael Rienstra, Jerome I Rotter, Aabida Saferali, Moore Benjamin Shoemaker, Edwin Silverman, Albert Vernon Smith, Nhlbi Trans-Omics For Precision Medicine (Topmed) Consortium; Pejman Mohammadi, Pejman Mohammadi, Stephane E Castel, Ivan Iossifov, Tuuli Lappalainen

Faculty, Staff and Student Publications

Exonic variants present some of the strongest links between genotype and phenotype. However, these variants can have significant inter-individual pathogenicity differences, known as variable penetrance. In this study, we propose a model where genetically controlled mRNA splicing modulates the pathogenicity of exonic variants. By first cataloging exonic inclusion from RNA-sequencing data in GTEx V8, we find that pathogenic alleles are depleted on highly included exons. Using a large-scale phased whole genome sequencing data from the TOPMed consortium, we observe that this effect may be driven by common splice-regulatory genetic variants, and that natural selection acts on haplotype configurations that reduce …

Effect Of Cutting Management On The Forage Production And Quality Of Tepary Bean (Phaseolus Acutifolius A. Gray), Travis W. Witt, Brian K. Northup, Timothy G. Porch, Santos Barrera, Carlos A. Urrea

Department of Agronomy and Horticulture: Faculty Publications

Tepary bean (Phaseolus acutifolius A. Gray) is an underutilized drought tolerant annual legume, originating from the Sonoran Desert, that may be a beneficial forage/hay for beef cattle in the Southern Great Plains of the US (SGP). The SGP has erratic rainfall and periods of intermittent drought exacerbated by high summer temperatures. In 2020 and 2021, a split-plot design was used to evaluate 13 genotypes of tepary bean and a forage soybean (control) at El Reno, OK, USA to compare production of plant biomass and forage nutritive value parameters under seven harvest regimes. Genotypes were used as the main plot …

Characterizing Host-Pathogen Interactions Between Zostera Marina And Labyrinthula Zosterae, Yaamini R. Venkataraman, Amanda Shore, Sukanya Dayal, James Sanghyun Lee, Mahsa Alidoost Salimi, Grace Crandall, Malina M. Loeher, Mark Stoops, Megan Swanger, Morgan E. Eisenlord, Kathryn L. Van Alstyne, Mark D. Fast, Colleen A. Burge, Maya L. Groner

Biology

Introduction: Seagrass meadows serve as an integral component of coastal ecosystems but are declining rapidly due to numerous anthropogenic stressors including climate change. Eelgrass wasting disease, caused by opportunistic Labyrinthula spp., is an increasing concern with rising seawater temperature. To better understand the host-pathogen interaction, we paired whole organism physiological assays with dual transcriptomic analysis of the infected host and parasite.

Methods:: Eelgrass (Zostera marina) shoots were placed in one of two temperature treatments, 11° C or 18° C, acclimated for 10 days, and exposed to a waterborne inoculation containing infectious Labyrinthula zosterae (Lz) or sterile seawater. At two- and …

Regulation Of Stress Responses And Membrane Morphology By Dspa In Synechocystis Sp. Pcc 6803, Rebekah Kathryn White

Theses and Dissertations

In the world of microorganisms, cyanobacteria are a uniquely skilled powerhouse. They participate in global nutrient and carbon cycling. They have the ability to photosynthesize, which allows them to use sunlight and carbon dioxide to produce cellular energy. However, photosynthesis has a lot of points were environmental stresses can interfere, increasing the chances of oxidative stress and nutrient imbalances that can amplify deleterious effects. One regulator in particular will be the target of this study. DspA, also known as Hik33, is a histidine kinase that perceives and regulates multiple overlapping stress responses. In this project, abiotic stress effects will be …

Improving Peripheral Nerve Regeneration Through Rehabilitation And Biomaterial-Based Drug Delivery Strategies, Yunfan Kong

Theses & Dissertations

Peripheral nerve injury (PNI) is a common problem worldwide, with trauma being a common cause. PNI can lead to loss of sensory and motor functions, chronic neuropathic pain, and mental health issues, significantly impacting patients' family life, work, and social situation. Recent studies revealed that beyond the topical injury site at peripheral nerves, PNIs can also induce dysfunctions in the central nervous system (CNS) by causing maladaptive plasticity, which will result in exaggeration and exacerbation of the pathological condition caused by primary injuries. The common therapy strategies for PNI treatment are using sutures, nerve autografts or conduits in cases requiring …

Crop Monitoring And Nutrient Prediction Using Satellite Imagery And Soil Data, Olatunde D. Akanbi, Brian Gonzalez Hernandez, Erika I. Barcelos, Arafath Nihar, Laura S. Bruckman, Yinghui Wu, Jeffrey Yarus, Roger H. French

Student Scholarship

No abstract provided.

Maximum Size-Density Relationships In Mixed-Species And Monospecific Stands Of The Southeastern United States, Maxwell Robert Schrimpf

Theses and Dissertations

Maximum size-density relationships (MSDR) are used to quantify differences across sites in the number of trees of a given size and species that can be supported per hectare. These relationships are important to managers who are trying to maximize basal area and wood volume. In my study, I examined MSDR across Alabama, Georgia, Louisiana, and Mississippi using US Forest Service, Forest Inventory and Analysis (FIA) data. I determined the impact of species-specific, specific gravity, functional traits, and environmental factors on MSDR using a quantile regression approach. Overall, I found that climatic factors had the greatest influence on MSDR, and that …

Effect Of Tamoxifen On Proteome Expression During In Vitro Myogenesis In Murine Skeletal Muscle C2C12 Cells, Emily A. Morris, Ahlenne Abreu, S. P. Scordilis

Biological Sciences: Faculty Publications

Tamoxifen (TMX), a selective estrogen receptor modulator, is commonly used in the treatment of hormone-responsive cancers. However, the effects of TMX in anabolic tissues harboring estrogen receptors, such as skeletal muscle, are poorly understood. We report a tandem mass-tag approach to TMX-treated myogenesis in C₂C₁₂ cells, a well-characterized model of in vitro murine skeletal muscle differentiation. A longitudinal analysis of >10,000 proteins identified in untreated C₂C₁₂ myogenesis revealed a novel subset of 1,062 myogenically regulated proteins. These proteins clustered into five distinct longitudinal expression trends which significantly overlap those obtained in similar analyses performed …

Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a bacterium that is responsible for colibacillosis in birds. However, information about broiler breeder APEC isolates is limited, but the data is critical due to the transfer of this bacteria down the production pyramid to progenies resulting in high mortality. Therefore, we evaluated the phenotypic virulence characteristics of 28 isolates using embryo lethality and day-old chick challenge assays. Also, the in vitro adhesion and invasion potential of selected nine isolates were identified. Results showed more than 1/3^rd of the isolates were highly virulent and the virulence increased as the number of virulence-associated genes …

Evaluation Of Heat Abatement Use, Vaginal Temperature, And Activity Of Pregnant, Lactating Holstein Cows Housed On Pasture With Or Without The Choice Between Shade And Sprinklers For Heat Abatement., Kevin M. Braman

Theses and Dissertations

The objective of this thesis was to determine how pregnant, lactating Holstein cows on pasture interact with shade and sprinklers when offered a choice or no-choice between methods. Authors hypothesized that cows would use shade more then sprinklers, but sprinklers would be more effective at cooling cows. Additionally, authors predicted cows using shade more would have decreased lying bouts and steps and an increase in time lying, compared to cows choosing sprinkler. Results indicate cows will use both methods when offered alone or together. However, cows will choose to be exposed or in shade at lower temperature humidity index values, …

The Interaction Between Predation And Sound Pollution On The Internal And External Microbiome Of The Grasshoppers Melanopus Sanguinipes, Alanna Bond

Theses and Dissertations

Anthropogenic activities such as construction and agriculture directly affect its surrounding environments. Many studies have focused on how megafauna and flora are impacted by human activities, however, relatively little is known how new occupation of land affects smaller organisms such as insects and microbes. Additionally, other stressors such as predation can impact host physiology. The grasshopper species Melanopussanguinipes was chosen to be used in a study to measure how stress caused by artificial sound and predation can alter exoskeleton and gut microbiomes once exposed to these stressors.

Molecular Mechanisms Of Novel Brain-Penetrating Oxime Acetylcholinesterase Reactivators On Sarin Surrogate-Induced Toxicities, Darby Caroline Stanford

Theses and Dissertations

Organophosphorus compounds (OPs), both nerve agents and insecticides, may induce damage leading to long-term neurological deficits. Oxime 20, the lead compound from a platform of substituted phenoxyalkyl pyridinium oximes (US Patent 9,227,937), is an oxime that reactivates OP-inhibited acetylcholinesterase (AChE) and has displayed neuroprotection within the brain of rats in in vivo tests where the currently approved treatment, pralidoxime (2-PAM), has not. This research investigated the neuroprotective effect of novel oxime therapy. Gene expression in rat piriform cortex was analyzed to determine if neurotoxicity, stress, and synaptic plasticity-associated genes were down-regulated by Oxime 20 following a sublethal dose of sarin …

Leaf Area Index And Aboveground Biomass Estimation Of Populus And Its Hybrids Using Terrestrial Lidar, Surya Adhikari

Theses and Dissertations

Short rotation woody crops (SRWC) eastern cottonwood (Populus deltoides) and hybrid poplar plantations were established in 2021 in Pontotoc and Oktibbeha counties of Mississippi to study the biomass potential of SRWC for biofuel production. We used a novel backpack LiDAR system to measure forest metrics and harvested sample trees to build aboveground biomass (AGB) and leaf area index (LAI) equations. The results showed that LiDAR-derived variables accurately estimated aboveground biomass (R² =0.81 and 29.22 % RMSE). However, the LAI estimation results showed that the LiDAR metrics moderately explained field measurements of LAI (R² =0.31 and 18.05% …