Life Sciences Commons^™

Assessing The Effects Of Live Oysters And Sampling Gear On Biodiversity Metrics Of Reef-Associated Benthic And Nekton Assemblages, Finella Campanino

LSU Master's Theses

Oysters are declining globally, resulting in an increase in oyster reef restoration, with many efforts prioritizing maintenance of reef-associated biodiversity. Understanding the role that reef-specific characteristics play in supporting biodiversity and how sampling gear influences measurements of biodiversity can inform design and management of restoration projects. I examined the relationship between live oyster volume and reef-associated benthic and nekton assemblages (hereafter reef assemblages) and how sampling gears influence our understanding of biodiversity on intertidal Crassostrea virginica reefs near Cocodrie, Louisiana. I quantified substrate characteristics, assemblages via trays, and water-column characteristics across four reefs and four seasons in 2022-2023, and compared …

Nmda Receptor Inhibition On Rodent Optimal Decision-Making In The Diminishing Returns Task, Seth Foust

Research Psychology Theses

There has been growing interest in using N-methyl-D-aspartate (NMDA) receptor antagonists as treatments for mood disorders, but there is still much to learn about their cognitive effects. Research shows NMDA receptors can affect decision-making, and the antagonist MK-801 has had varying effects in rodents. Specifically, some have reported impairments in working memory while foraging behaviors remained intact, while others have demonstrated changes in choice behavior related to delay or risk in behavior tasks. We investigated the role of NMDA receptors in the specific paradigm of optimal decision-making to further confirm MK-801’s effects and to explore whether inhibiting NMDA receptors alters …

Swarmdeepsurv: Swarm Intelligence Advances Deep Survival Network For Prognostic Radiomics Signatures In Four Solid Cancers, Qasem Al-Tashi, Maliazurina B Saad, Ajay Sheshadri, Carol C Wu, Joe Y Chang, Bissan Al-Lazikani, Christopher Gibbons, Natalie I Vokes, Jianjun Zhang, J Jack Lee, John V Heymach, David Jaffray, Seyedali Mirjalili, Jia Wu

Faculty, Staff and Student Publications

Survival models exist to study relationships between biomarkers and treatment effects. Deep learning-powered survival models supersede the classical Cox proportional hazards (CoxPH) model, but substantial performance drops were observed on high-dimensional features because of irrelevant/redundant information. To fill this gap, we proposed SwarmDeepSurv by integrating swarm intelligence algorithms with the deep survival model. Furthermore, four objective functions were designed to optimize prognostic prediction while regularizing selected feature numbers. When testing on multicenter sets (n = 1,058) of four different cancer types, SwarmDeepSurv was less prone to overfitting and achieved optimal patient risk stratification compared with popular survival modeling algorithms. Strikingly, …

Hedgehog Signaling As An Underlying Mechanism For Midline Modification During Asexual Reproduction In Convolutriloba Longifissura Acoels, James M. Sikes Ph.D., Jaclyn Cherry

Master's Theses

The mechanisms that control midline specification and modification during post-embryonic development are some of the least understood in developmental biology.  Because acoel flatworms have varied modes of asexual reproduction which can involve the dramatic modification of their orthogonal axes and midline, we have chosen the acoel species Convolutriloba longifissura as a model to study the mechanisms behind midline modification.  C. longifissura asexually reproduces through a double fission process where novel midlines arise within laterally located tissues while the adult midline is destroyed prior to an initial transverse fission event, which is then followed by a second longitudinal fission.  Previous work …

Parasites Versus Predation: The Role Of Chronic And Acute Parasite Exposure In Infection Risk And Anti-Predator Behavior, Delaney Farrell

All HCAS Student Capstones, Theses, and Dissertations

Parasites with complex, multi-host lifecycles often engage in host behavior manipulation to increase transmission between successive hosts. In intermediate fish hosts, previous research has measured increased frequency of conspicuous behaviors, decreased swimming performance, and reduced antipredator behavior, which would collectively increase the fish’s risk of predation. In ecosystems where this type of parasite increased trophic transmission (PITT) occurs, parasites can play a substantial role in food webs. In this study, I investigate how chronic versus acute exposure to the trematode Euhaplorchis sp. A. affects the antipredator behavior of the Gulf killifish Fundulus grandis. Using a fully crossed design, I …

Characteristics Of The Moringa Mother Tree In The Population Of East Flores, East Nusa Tenggara, Adi Setiadi, Evert Yulianes Hosang

Jurnal Kultivasi

The moringa plant (Moringa oleifera Lam.) is a member of the Moringaceae family that grows in Indonesia. Moringa plants have many benefits, both as a source of food, medicine, and cosmetics. The development of moringa plants in Indonesia has not been carried out because it is still constrained by the absence of a mother tree and a source of moringa seed gardens. This study aims to identify morphological characters and production potential in two populations of moringa plants. The research was conducted in August 2021 in East Flores District, East Nusa Tenggara Province. The research was conducted by determining the …

Vitamin C And Total Soluble Solid Content Of Crystal Guava At Different Storage Duration And Ripeness, Abdul Khafid, Yulita Nurchayati, Endah Dwi Hastuti, Nintya Setiari

Jurnal Kultivasi

Crystal guava (Psidium guajava var. 'Crystal') fruit is in great demand because of its delicious taste and high nutritional content. Storage aims to prevent postharvest damage to the fruit. However, storage that is too long causes morphological damage and decreased nutrients. This study aims to determine the effect of storage duration, fruit ripeness stage and the interaction between both factors on vitamin C and total soluble solids (TSS) content of crystal guava, and determine which treatment can produce the highest vitamin C and TSS. Fruits harvested simultaneously with three levels of ripeness based on the skin color: unripe fruit is …

Universal Dna Methylation Age Across Mammalian Tissues, A. T. Lu, Jessica Lynn Petersen, Steve Horvath, Et Al.

Department of Animal Science: Faculty Publications

Aging, often considered a result of random cellular damage, can be accurately estimated using DNA methylation profiles, the foundation of pan-tissue epigenetic clocks. Here, we demonstrate the development of universal pan-mammalian clocks, using 11,754 methylation arrays from our Mammalian Methylation Consortium, which encompass 59 tissue types across 185 mammalian species. These predictive models estimate mammalian tissue age with high accuracy (r > 0.96). Age deviations correlate with human mortality risk, mouse somatotropic axis mutations and caloric restriction. We identified specific cytosines with methylation levels that change with age across numerous species. These sites, highly enriched in polycomb repressive complex 2-binding locations, …

A Review With Updated Perspectives On In Vitro And In Vivo Wound Healing Models, Murni Nazira Sarian, Nabilah Zulkefli, Shazeli Zain, Sandra Maniam, Sharida Fakurazi

Turkish Journal of Biology

A skin wound or perforation triggers a series of homeostatic reactions to safeguard internal organs from invasion by pathogens or other substances that could damage body tissues. An injury may occasionally heal quickly, leading to the closure of the skin's structure. Healing from chronic wounds takes a long time. Although many treatment options are available to manage wound healing, an unmet therapy need remains because of the complexity of the processes and the other factors involved. It is crucial to conduct consistent research on novel therapeutic approaches to find an effective healing agent. Therefore, this work aims to cover various …

Effectiveness Of Arbuscular Mycorrhizal Fungi In Increasing Growth And Yield Of Maize Overlaid On Oil Palm Aged 4 Years, Cucu Suherman, Sri Desi Nurliawati, Mira Ariyanti, Intan Ratna Dewi, Mochamad Arief Soleh

Jurnal Kultivasi

The intercropping system in oil palm plantations is an effort to optimize land, especially at the immature stages (IS), which have a large open space between the trees, so it can be used for cultivating annual crops such as maize. Oil palm trees are generally planted on marginal lands, such as Inceptisol, which generally lacks in phosphor (P). These problems can be reduced by applying arbuscular mycorrhizal fungi (AMF) to elevate P. This experiment was to determine the dosage and effectiveness of AMF that can improve the growth and yield of maize intercropped with a 4-year-old oil palm. The experiment …

The Effects Of Application Of Biochar From Oil Palm Empty Fruit Bunches On Chemical Properties Of Ultisols And The Growth Of Cacao Seedlings, Wulan Kumala Sari, Panji Abdul Malik

Jurnal Kultivasi

It is necessary to redevelop cacao (Theobroma cacao L.) commodity due to the decrease of cacao planting areas in Indonesia since the last decade. One of the ways is by providing a growing medium to produce cacao seedlings with good quality, such as by adding ameliorant i.e., oil palm empty fruit bunch (OPEFB) biochar to marginal soil of Ultisols. This research aimed to study and obtain the best OPEFB biochar dose to improve the chemical properties of Ultisols and the growth of cacao seedlings. This research was conducted in the Experimental Field of the 3rd Campus Andalas University from August …

Radiographic Measurements Correlate To Isolated Posterolateral Corner (Plc) Injury In A Novel Cadaveric Model, Joseph D. Henningsen, Scott Huff, Andrew Reichard, Andrew W. Froehle, Anil Krishnamurthy

School of Health and Exercise Sciences Faculty Publications

Introduction: Injury to the posterolateral corner (PLC) of the knee often requires surgical reconstruction. There remains no consensus on treatment for PLC injury, and, therefore, it is imperative to have a reproducible injury model to improve the general knowledge of PLC injuries. A novel cadaveric model of isolated PLC injury is proposed and evaluated using radiographic parameters as well as gross dissection.

Material and methods: All protocols were reviewed by the Human Investigation and Research Committee of the home institution and were approved. Translational force in a defined posterior and lateral direction was applied to cadaveric native knees to induce …

Aquatic Insect Communities Remain Impaired Following Large-Scale River Restoration, Jared Meek, Jackson Birrell, Teagan Mulford, Dennis K. Shiozawa, C. Riley Nelson

Western North American Naturalist

River manipulation and degradation can cause detrimental impacts on aquatic insect communities. River restoration projects seek to mitigate these human disturbances. Assessing the efficacy of river restoration remains an important goal for river managers and conservation biologists so that improved restoration methods can be used in the future. From 1999 to 2008, the Utah Reclamation Mitigation and Conservation Commission restored a 15-km channelized portion of the Provo River (Wasatch County, Utah, USA). Before (1999) and during (2002) the restoration project, macroinvertebrate samples were collected using modified kick nets from 3 sites within the restored area and 1 site outside the …

Antioxidant Potential Of Nanomaterials, David Gonzalez Flores, Javier Espino, Jose A. Pariente

Turkish Journal of Biology

Background/aim: The novel field of nanomaterials allows infinite possibilities in order to create antioxidant therapies. The present review is aimed to describe the state of art concerning on nanomaterials and their effects on reactive oxygen species (ROS) production. A wide range of nanoparticles has been designed for this purpose, and each one possesses some particular characteristics which allow these significant antioxidant results. Several in vivo and in vitro works state the ability of these nanoparticles to mimic the redox systems of the cells, and thus, the potential role of nanoparticles as antioxidant treatment for several diseases. Materials and methods: This …

Targeting Of Notch, Il-1, And Leptin Has Therapeutic Potential In Xenograft Colorectal Cancer, Rumeysa Özyurt, Ni̇lüfer Erkasap, Mete Özkurt, Serdar Mustafa Erkasap, Konstanti̇nos Di̇mas, Ayşe Çakir Gündoğdu, Engi̇n Ulukaya

Turkish Journal of Biology

Background/aim: Colorectal cancer (CRC) is a fatal malignancy type and its occurence still needs to be explored mechanistically. Notch, IL-1, and leptin crosstalk is reported to play a role in the proliferation, migration, and expression of proangiogenic molecules. In this study, we aimed to investigate the effect of inhibition of Notch, IL-1, and leptin on CRC. Materials and methods: To generate colorectal cancer tumor xenografts, 1 × 107 cells from exponentially growing cultures of HCT15 cells were injected subcutaneously, at the axillary region of the left and right rear flanks of forty NOD.CB17-Prkdcscid/J (NOD/SCID) female mice. The mice were then …

Soloxolone Methyl Induces Apoptosis And Oxidative/Er Stress In Breast Cancer Cells And Target Cancer Stem Cell Population, Eli̇f Ertürk, Oğuzhan Akgün, Yaren Yildiz, Pinar Kalkan, Oksana V. Salomatina, Nariman F. Salakhutdinov, Engi̇n Ulukaya, Ferda Ari

Turkish Journal of Biology

One of the most prevalent malignancies in women and one of the leading causes of cancer-related death is breast cancer. There is a need for new treatment approaches and drugs for breast cancer. Many studies show the high potential of triterpene compounds and their semisynthetic derivatives as anticancer agents due to their ability to induce apoptosis and suppress tumorigenesis. The effects of soloxolone methyl (SM), a semisynthetic derivative of 18-H-glycyrrhetinic acid, on the cytotoxicity and apoptosis of human breast cancer cell line (T-47D) and cancer stem cell (CSCs) population (mammospheres; CD44+/CD24-antigen) derived from breast cancer cells, were examined in this …

Fatiguing Joint Angle Does Not Influence Torque And Neuromuscular Responses Following Sustained, Isometric Forearm Flexion Tasks Anchored To Perceptual Intensity In Men, Dolores G. Ortega, Terry J. Housh, Robert Smith, Jocelyn E. Arnett, Tyler J. Neltner, John Paul V. Anders, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

This study examined the effects of joint angle (JA) on maximal voluntary isometric contraction (MVIC) and neuromuscular responses following fatiguing tasks anchored to RPE. Nine men (mean ± SD: age = 20.7 ± 1.2 yrs) performed forearm flexion MVICs at elbow JAs of 75^o and 125^o before and after sustained, isometric forearm flexion tasks to failure at fatiguing joint angles (FJA) of 75^o and 125^o anchored to RPE = 8. The amplitude and frequency of the electromyographic and mechanomyographic signals were recorded. Neuromuscular efficiency was calculated by dividing normalized torque by normalized electromyographic amplitude. A dependent …

Perioperative Pembrolizumab For Early-Stage Non-Small-Cell Lung Cancer, Heather Wakelee, Moishe Liberman, Terufumi Kato, Masahiro Tsuboi, Se-Hoon Lee, Shugeng Gao, Ke-Neng Chen, Christophe Dooms, Margarita Majem, Ekkehard Eigendorff, Gastón L Martinengo, Olivier Bylicki, Delvys Rodríguez-Abreu, Jamie E Chaft, Silvia Novello, Jing Yang, Steven M Keller, Ayman Samkari, Jonathan D Spicer, Keynote-671 Investigators

Faculty, Staff and Student Publications

BACKGROUND: Among patients with resectable early-stage non-small-cell lung cancer (NSCLC), a perioperative approach that includes both neoadjuvant and adjuvant immune checkpoint inhibition may provide benefit beyond either approach alone.

METHODS: We conducted a randomized, double-blind, phase 3 trial to evaluate perioperative pembrolizumab in patients with early-stage NSCLC. Participants with resectable stage II, IIIA, or IIIB (N2 stage) NSCLC were assigned in a 1:1 ratio to receive neoadjuvant pembrolizumab (200 mg) or placebo once every 3 weeks, each of which was given with cisplatin-based chemotherapy for 4 cycles, followed by surgery and adjuvant pembrolizumab (200 mg) or placebo once every 3 …

Indigenous Lakadong Turmeric Of Meghalaya And Its Future Prospects, Manjit Kumar Ray, Dipak K. Santra, Piyush Kumar Mishra, Saurav Das

Department of Agronomy and Horticulture: Faculty Publications

Turmeric (Curcuma longa) has long been used in traditional Indian medicine. India accounts for 80% of total global turmeric production. Lakadong turmeric gets its name from the tiny village of Lakadong, which is located in the foothills of the Jaintia Hills in Meghalaya, India. It is known for having a high curcumin content of more than 7%, as opposed to 2 - 4% in regular varieties. The tribes of this region brought Lakadong turmeric from the forest and domesticated it for medicinal purposes centuries ago. Growth in local coal industries and a gradual decline in the market have …

The Effects Of Anti-Pd-L1 Monoclonal Antibody On The Expression Of Angiogenesis And Invasion-Related Genes, Cansu Babahan, Samira Abdi Abgarmi, Fatma Gi̇zem Sonugür, Müge Öçal Demi̇rtaş, Hakan Akbulut

Turkish Journal of Biology

Background/aim: The role of PD-L1 in regulating the immunosuppressive tumor microenvironment via its binding on PD-1 receptors is extensively studied. The PD-1/PD-L1 axis is a significant way of cancer immune escape, and PD-L1 expression on tumor cells is suggested as a predictive marker for anti-PD-1/PD-L1 monoclonal antibodies (MoAbs). However, the tumor-intrinsic role of PDL1 is not known well. Therefore, we aimed to investigate the effects of anti-PD-L1 antibodies on the expression of angiogenesis and metastasis-related genes in tumor cells. Materials and methods: The experiments were done with prostate cancer and melanoma cells with low PD-L1 expression (<5%) and prostate and breast cancer cells with high PD-L1 expression (>50%). The gene …

Desperate Larva Or Death Before Dishonor: Can Old Coral Larvae Replenish Degraded Reefs?, Nepsis García Lara

All HCAS Student Capstones, Theses, and Dissertations

The recovery of coral populations depends largely on larval recruitment. Coral larvae settle in response to environmental cues that indicate habitat quality. Newly competent larvae typically avoid settling on substrates with high macroalgal cover and sedimentation, and thus might never recruit to degraded reefs, hindering their recovery. What is unknown is if settlement preferences change as larvae age. In the absence of suitable settlement cues, lecithotrophic larvae delay settlement and might do this until either dying (Death Before Dishonor Hypothesis) or becoming less discriminatory and settling regardless of their specific habitat requirements (Desperate Larva Hypothesis). To test these hypotheses in …

Antileukemic Potential Of Nile Blue-Mediated Photodynamic Therapy On Hl60 Human Myeloid Leukemia Cells, Serçi̇n Özlem Çalişkan, Aynur Karadağ, Bariş Uzunok, Numan Taşpinar, Berna Akin, Meti̇n Çalişkan, Rahşan Ilikçi Sağkan

Turkish Journal of Biology

Background/aim: Photodynamic therapy (PDT) has received great attention over the past decade in the treatment of diseases such as leukemia which is a cancer of the blood and bone marrow cells that causes a significant number of deaths worldwide. In this study, it was aimed to investigate the effects of Nile blue-mediated PDT (NB-mediated PDT) on HL60 cells. Materials and methods: The effect of NB-mediated PDT on cell proliferation was evaluated with cell volume analysis using flow cytometry at 24 h. Cell apoptosis, ROS production, mitochondrial membrane potential, and cell cycle analysis were evaluated using annexin V-FITC, H2 DCFDA, JC-1, …

Multifocal And Multicentric Glioblastoma: Imaging Signature, Molecular Characterization, Patterns Of Spread, And Treatment, Maguy Farhat, Gregory N Fuller, Max Wintermark, Caroline Chung, Vinodh A Kumar, Melissa Chen

Faculty, Staff and Student Publications

Multifocal and multicentric glioblastoma (GBM) or collectively, m-GBM, is an imaging diagnosis present in up to 34% of patients with GBM. Compared to unifocal disease, patients with m-GBM have worse outcomes owing to the enhanced aggressive nature of the disease and its resistance to currently available treatments. To improve the understanding of its complex behavior, many associations have been established between the radiologic findings of m-GBM and its gross histology, genetic composition, and patterns of spread. Additionally, the holistic knowledge of the exact mechanisms of m-GBM genesis and progression is crucial for identifying potential targets permitting enhanced diagnosis and treatment. …

Scaling Up Of Site Carbon Dynamics To Predict The Carbon Dynamics In Kazakhstan, Central Asia, B. K. Wylie, Tagir Gilmanov, S. L. Stensaas, N. Z. Saliendra, D. A. Johnson, K. Akshalov, S. B. Frank, L. Zhang, R. F. Doyle, E. A. Laca, Montague W. Demment

IGC Proceedings (1977-2023)

Climate and management determine whether rangelands are net carbon sources or carbon sinks. Regional carbon dynamics of the Kazakh Steppe has not previously been documented. The objective of this study is to quantify the regional carbon flux dynamics of these extensive steppes.

A New System For Plant Experiments On Biodiversity Or Multi-Species Competition, John Connolly, L. Kirwan

IGC Proceedings (1977-2023)

Considerable discussion in recent years has focused on the design of competition and biodiversity experiments (Connolly et al., 2001a; Allison, 1999). Few agronomic experiments with >2 plant species have been conducted in greenhouse conditions (Gibson et al, 1999) or in the field (Connolly et al., 2001b). In many experiments the effects of density and initial species size have been confounded. The effects of species richness and evenness also are confounded frequently. The proposed system provides a framework of design and analysis, in which to address questions of function at community level and of structure and competition at …

Native Grasses Seeded Into A Cool-Season Pasture Encouraged By Low Resource Availability, J. E. Doll, R. L. Cates, R. D. Jackson

IGC Proceedings (1977-2023)

Native prairie of the Upper Midwest, which was dominated by warm-season (C₄) grasses, now exists as relatively small relict and restored patches (Curtis 1959). Re-introduction of natives into grazed agroecosystems would promote genetic, species, and landscape diversity. Extensive re-introduction of C₄ grasses will require a shift away from the paradigm of maximizing production because C₄ grasses have higher C:N ratios than C₃ grasses rendering them inferior forage species. Nonetheless, there is great interest amongst the grazing community of the Upper Midwest in establishing native grasses as a means of improving wildlife habitat and increasing belowground …

The Ag-Biota Project: A Preliminary Assessment Of Potential Indicators Of Biodiversity In Agricultural Grasslands, Gordon Purvis, A. Anderson, A. J. Helden, L. Kirwan

IGC Proceedings (1977-2023)

In compliance with European commitments to the Convention on Biological Diversity, the conservation and enhancement of biodiversity within agricultural land is a primary objective of current agri-environmental measures. However, there is a widespread lack of information concerning the effectiveness of agri-environmental schemes in Europe, (Kleijn & Sutherland, 2003). In large part, this is due to uncertainties about selection of appropriate biological indicators of biodiversity (Buchs, 2003; Duelli & Obrist, 2003).

Effects Of Plant Species Diversity In Multifunctional Grasslands On Avian Communities, K. K. Bakker, K. F. Higgins

IGC Proceedings (1977-2023)

Grasslands, planted or native sod, can provide multiple simultaneous functions (e.g., hay-forage production, biomass fuel products, bird nesting cover, soil and water conservation). Bird use of and abundance in grasslands is dependent on vegetation structure, size, and surrounding landscape (Bakker et al., 2002) but little is known about avian relationships to plant species diversity. We evaluated the relationship between plant species diversity and avian community structure in 5 grassland types. The results have application to grazing, haying, burning, and conservation practices applied to native and sown grassland stands and to conservation strategies for declining grassland bird populations.

Diversity And Adaptation Of Perennial Plants From North Africa: Legumes And Grasses, Aissa Abdelguerfi, M. Laouar, K. Abbas, M. M'Hammedi Bouzina

IGC Proceedings (1977-2023)

A variety of climates, soil types and reliefs characterise North Africa (Algeria, Morocco, and Tunisia). These natural conditions have produced a large diversity of environments, landscapes, plant formations and flora. Several factors have contributed to the spread of a wide floristic variety: the influence of the Mediterranean in the north and in the north-east, the Atlantic in the west, and the Sahara in the south, as well as the presence of mountain ranges, particularly the Rif, the Tell Atlas, the Sahara Atlas, the Middle Atlas, the High Atlas, and the Anti-Atlas. This diversity of landscapes, environments and ecosystems has generated …

Comparison Of Compositional Changes In Multi-Species Grass/Legume Mixture Experiments Across Three Nordic Countries (Iceland, Sweden And Finland) Over Two Years, Caroline Brophy, Anne-Maj Gustavsson, A. Helgadottir, Oiva Nissinen, J. Connolly

IGC Proceedings (1977-2023)

A multi-site experiment was established at 39 sites across Europe, Australia and Canada within COST Action 852 to: (1) assess the benefits of grass/legume mixtures over monocultures, (2) test the stability of mixtures and (3) evaluate the consistency of the observed patterns over broad environmental gradients. This paper compares the results from 3 Nordic sites using the same plant species: Korpa Experimental Station in Iceland, Lapland Research Station in Finland and Piteå in Sweden to investigate if compositional changes in mixtures are consistent across the sites.