Life Sciences Commons^™

Cell-Based Experimental Evidence To The Anti-Covid-19 Potential Of Ashwagandha And Honeybee Propolis Ingredients, Renu Wadhwa, Durai Sundar, Yoshiyuki Ishida, Keiji Terao, Sunil C. Kaul

Research Symposium

Background: The COVID-19 pandemic emerged in December 2019 by a novel strain of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) has led to new endeavours in repurposing of existing drugs, anti-COVID-19 vaccine and drug development. Natural products, due to their general safety and wider availability, have attracted research and public attention. In this study, we report anti-COVID potential of compounds from honeybee propolis and Ayurvedic herb, Ashwagandha. Effect of active ingredients was studied on human cell surface receptors (ACE-2:Angiotensin Converting Enzyme-2/Spike protein and TMPRSS2:Transmembrane Protease Serine 2), critical for virus infection and virus main protease (Mpro, essential for virus replication), …

Dpyd Pathogenic Variants Associated With Fluoropyrimidines Toxicity, Diana Cristina Pérez-Ibave, Noé Israel Oliva-García, Irasema Ramos-Martínez, Francisco Javier Villarreal Alvarado, Valeria Jimena Gómez Ordaz, Jonatán Isaí Cortes Alfaro, Carlos Horacio Burciaga-Flores, Juan Francisco González-Guerrero, Oscar Vidal-Gutiérrez, Maria De Lourdes Garza-Rodriguez

Research Symposium

Background: Genetic variants in dihydropyrimidine dehydrogenase gene (DPYD) coding for the key enzyme (DPD) of fluoropyrimidines (FPs) catabolism. DPYD contributes to the development of severe FPs-related toxicity, and pathogenic DPYD variants detection reduces side effects and complications associated with FP-toxicity. The allelic frequency of these variants in the Mexican population is currently unknown.

Methods: The study was carried out at the Centro Universitario Contra el Cáncer (CUCC) of the Universidad Autónoma de Nuevo León (UANL) in Monterrey México. Genomic DNA was isolated from 154 subjects using the QIAamp DNA Blood Midi kit (QIAGEN) following the manufacturer's recommendations. We …

Differential Cortical Monoamines Release During Exercise In Rats Chronically Implanted With Microdialysis Probes, Christine G. Gerin

Research Symposium

Physical exercise is known to positively influence mood, to reduce anxiety and to improve reaction to stress. Cerebral monoaminergic systems are thought to underly the neurochemical influence of exercise on mood and behavior. We hypothesized that beneficial effects of exercise can be shown by variations of neurotransmitters release in the cerebral cortex. We aimed at demonstrating that there is a temporal relationship between release of plasma and cerebral monoamines (A, NA, 5-HT, DA) and spontaneous running exercise in rats. Ten Spague Dawley rats (250 g) were chronically implanted (for up to 39 days) with a left common carotid cannula and …

Challenges In Genetic Counseling In Hereditary Cancer Syndromes In A Mexican Oncologic Center, Diana Cristina Perez-Ibave, Diana Cristina De Lourdes Perez Ibave, María Fernanda Noriega-Iriondo, Omar Alejandro Zayas-Villanueva, Fernando Alcorta-Nuñez, Juan Francisco González-Guerrero, Adelina Alcorta-Garza, David Hernandez-Barajas, Oscar Vidal-Gutierrez, Carlos Horacio Burciaga-Flores

Research Symposium

Background: In Mexico, hereditary cancer is underdiagnosed, medical geneticists give genetic counseling, but the access is limited due to the socio-economic characteristics of the population. The CUCC (Centro Universitario Contra el Cáncer) Early Cancer Detection Clinic (CECIL) created a model in which patients without cancer are enrolled in a prevention cancer screening program.

Methods: From 2016 to 2021, 3014 patients were enrolled in the prevention program. Patients were evaluated with a hereditary cancer risk survey before a consultation. Those with at least one familial hereditary risk positive answer were assessed in a consultation. We also included patients with cancer diagnoses …

The First Record Of Orthochirus Glabrifrons (Kraepelin, 1903) (Scorpiones: Buthidae) From The United Arab Emirates, František Kovařík, Ersen A. Yağmur, Alexander Ullrich, Balázs Buzás

Euscorpius

Orthochirus glabrifrons (Kraepelin, 1903) (Scorpiones: Buthidae) was described from Oman (Muscat). Here, we summarize known localities from Oman as well as records from the United Arab Emirates, which is the new country record for this species. Illustrations of morphology of both sexes are given together with a map of distribution. A lectotype of Orthochirus glabrifrons (Kraepelin, 1903) is designated. Paraorthochirus kaspareki Lourenço & Huber, 2000 and Paraorthochirus kinzelbachi Lourenço & Huber, 2000 are synonymized with Orthochirus glabrifrons (Kraepelin, 1903), syn. n.

Dynamic Reconfiguration Of Switchgrass Proteomes In Response To Rust (Puccinia Novopanici) Infection, Nathan A. Palmer, Sophie Alvarez, Michael J. Naldrett, Anthony Muhle, Gautam Sarath, Serge J. Edmé, Satyanarayana Tatineni, Robert B. Mitchell, Gary Yuen

Department of Plant Pathology: Faculty Publications

Switchgrass (Panicum virgatum L.) can be infected by the rust pathogen (Puccinia novopanici) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 …

Cercocarpus Ledifolius Var. Intricatus ‘Doubledown’, Asmita Paudel, Youping Sun, Larry A. Rupp

Plants, Soils, and Climate Faculty Publications

Bonsai (tray landscape, potted scenery, potted landscape, miniature trees, and rockery) is an artistic horticulture practice of developing aesthetically formed trees and landscapes in miniature with appropriately aesthetic containers. This has been practiced over a few thousand years in oriental cultures, including the ancient Chinese tradition of penzai or penjing, from which the art originated; the miniature living landscapes of Vietnamese hòn non bộ; and the Japanese variations of bonsai and “tray planting” (Gustafson 1995). To produce bonsai plants that share similar shapes and styles of mature, full-size trees, cultivation techniques are used, including leaf trimming, …

Genome Sequences Of Six Cluster Ct And Two Cluster Dj Bacteriophages That Infect Gordonia Rubripertincta, Xavier F. Rodriguez, Daniel C. Williams, Catherine P. Chia

School of Biological Sciences: Faculty Publications

We report the genome sequences of eight bacteriophages isolated using Gordonia rubripertincta NRRL B-16540-SEA. Based on gene content similarity to phages in the Actinobacteriophage database, six of the phages are assigned to phage cluster CT while two are assigned to cluster DJ.

Principles Of Chemistry Ii, Janet L. Shaw

KSU Distinguished Course Repository

Second course in a two-semester sequence covering the fundamental principles and applications of chemistry designed for science majors.

With a pre-requisite of CHEM 1211: Principles of Chemistry I, this course is part of a two-semester sequence covering principles and applications of chemistry. The course is designed to help students apply simplified models to complex systems to build critical thinking and problem-solving skills. A major goal of this course is to help students become self-directed learners thereby preparing them for whatever career path they choose. Although designed for science majors who typically complete the course during their freshman year, pre-health majors …

New Foods Produced With Genome-Editing Technique In The Global Marketplace, Stéphan Marette, Anne-Célia Disdier, John C. Beghin

Cornhusker Economics

This Cornhusker Economics article builds upon a previous Cornhusker Economics article of September 8, 2021, on the heterogenous willingness to pay (WTP) for genome edited (GenEd) apples in France and the US (Beghin, 2022). Using graphical illustration, this new article summarizes in layman's terms two related economic analyses by Marette et al. (2022) and (2023). These analyses investigate Research and Development (R&D) investment in food innovations based on New Plant Engineering Techniques (NPETs), such as GenEd and traditional hybridization methods, and then in the context of international trade. With trade, innovators compete globally with their food novelties in each other’s …

Self-Organization For Community Resilience In An Invisible Agricultural Community, Anna Erwin, Chelsea A. Silva, Zhao Ma

School of Earth, Environmental, & Marine Sciences Faculty Publications

This study investigates how self-organizing efforts by residents of informal settlements, primarily migrant and informal farmworkers, shape community resilience in Majes, a water-scarce irrigation district in the Atacama Desert of Peru. We collected 45 semi-structured interviews with residents and authorities in Majes and analyzed findings through a framework of self-organizing. Analyses revealed that self-organizing by residents of informal settlements incorporated the three components of White’s theory of Community Agency and Community Resilience, which contends that marginalized communities increase resilience by fostering a commons praxis, practicing a prefigurative politics, and developing opportunities for economic autonomy. We also found …

Repurposing Normal Chromosomal Microarray Data To Harbor Genetic Insights Into Congenital Heart Disease, Nephi Walton, Hoang Nguyen, Sara Procknow, Darren Johnson, Alexander Anzelmi, Patrick Jay

Department of Medicine Faculty Papers

About 15% of congenital heart disease (CHD) patients have a known pathogenic copy number variant. The majority of their chromosomal microarray (CMA) tests are deemed normal. Diagnostic interpretation typically ignores microdeletions smaller than 100 kb. We hypothesized that unreported microdeletions are enriched for CHD genes. We analyzed "normal" CMAs of 1762 patients who were evaluated at a pediatric referral center, of which 319 (18%) had CHD. Using CMAs from monozygotic twins or replicates from the same individual, we established a size threshold based on probe count for the reproducible detection of small microdeletions. Genes in the microdeletions were sequentially filtered …

Video Based Estimation Of Surface Currents In A Tidal Inlet, Benjamin Middour, George Voulgaris, Douglas Cahl

Faculty Publications

Oceanic surface currents are estimated from analysis of short duration video obtained by a nadir facing camera installed on a bridge over a tidal inlet. Data analyzed consisted of 13 two-minute video clips captured approximately every 40 min over a period of 8 hr, covering a significant portion of the tidal cycle. Surface current speed and direction estimates are obtained from each collected video using (i) a method originally developed for analysis of imagery from drones (CopterCurrents) and (ii) a particle tracking method. The horizontal (u, v) surface current velocity components and total horizontal velocity magnitude, obtained from the two …

Differential Effects Of Pancreatic Cancer-Derived Extracellular Vesicles Driving A Suppressive Environment, Anurag Purushothaman, Jacqueline Oliva-Ramírez, Warapen Treekitkarnmongkol, Deivendran Sankaran, Mark W Hurd, Anirban Maitra, Subrata Sen

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) cells display extensive crosstalk with their surrounding environment to regulate tumor growth, immune evasion, and metastasis. Recent advances have attributed many of these interactions to intercellular communication mediated by small extracellular vesicles (sEVs), involving cancer-associated fibroblasts (CAF). To explore the impact of sEVs on monocyte lineage transition as well as the expression of checkpoint receptors and activation markers, peripheral blood monocytes from healthy subjects were exposed to PDAC-derived sEVs. Additionally, to analyze the role of sEV-associated HA in immune regulation and tissue-resident fibroblasts, monocytes and pancreatic stellate cells were cultured in the presence of PDAC sEVs …

Author Correction: Synthetic Oct-A Blood Vessel Maps Using Fundus Images And Generative Adversarial Networks, Ivan Coronado, Samiksha Pachade, Emanuele Trucco, Rania Abdelkhaleq, Juntao Yan, Amanda Jagolino-Cole, Roomasa Channa, Sunil A Sheth, Luca Giancardo

Faculty, Staff and Student Publications

No abstract provided.

Antibodies To Coagulase Of Staphylococcus Aureus Crossreact To Efb And Reveal Different Binding Of Shared Fibrinogen Binding Repeats, Federico Bertoglio, Ya-Ping Ko, Sheila Thomas, Liliana Giordano, Francesca Romana Scommegna, Saskia Polten, Srishtee Arora, Michael Hust, Magnus Höök, Livia Visai

Faculty, Staff and Student Publications

Staphylococcus aureus pathology is caused by a plethora of virulence factors able to combat multiple host defence mechanisms. Fibrinogen (Fg), a critical component in the host coagulation cascade, plays an important role in the pathogenesis of this bacterium, as it is the target of numerous staphylococcal virulence proteins. Amongst its secreted virulence factors, coagulase (Coa) and Extracellular fibrinogen-binding protein (Efb) share common Fg binding motives and have been described to form a Fg shield around staphylococcal cells, thereby allowing efficient bacterial spreading, phagocytosis escape and evasion of host immune system responses. Targeting these proteins with monoclonal antibodies thus represents a …

Impact Of Physical Inactivity On Glucose Metabolism And Skeletal Muscle Secretions In Healthy Trained Rats And Potential Counteracting Effects Of Exercise And Protocatechuic Acid, Hira Shakoor

Thesis/ Dissertation Defenses

Modern societies have significantly transformed people's lifestyles, leading to a substantial increase in physical inactivity, which concerns 23% of adults and 81% of adolescents worldwide. Physical inactivity is a major factor contributing to the emergence of chronic diseases, most prominently Type 2 Diabetes (T2D), which exhibits a high prevalence in many countries. T2D is characterized by elevated blood glucose levels due to cells’ responsiveness to insulin. The shift towards inactivity likely occurred more rapidly than the genome's capacity to adapt, resulting in the metabolic dysfunctions underlying the development of T2D. Skeletal muscle is crucial in the onset of these metabolic …

Fundamental Study Of Ionic Liquid Physicochemical Effects On Thermal Stability Of Model Biological Macromolecules, Austin Keith Clark

Theses and Dissertations

Ionic Liquids (ILs) are substances with a unique physical attribute compared to that of solid ionic salts. At room temperature, ILs are molten salts that have a variety of physical effects that can play a role in their impact on other molecules, as solvents or solutes. They can play the role of the solvent in a variety of applications, from biofuels to organic catalysis or as excipients in pharmaceutical formulations. These ILs have a desirable use as solvents due to their ability to be tunable substances. Changing the cation or anion of the IL causes a change in its physical …

Two New Species Of Tulostoma (Agaricales, Basidiomycota) From The Neotropics, Alexandro Lima, Gislaine Melanda, Paulo Marinho, T A, María Paz Martín, Iuri Baseia

Turkish Journal of Botany

Tulostoma catimbauense and T. deltaconcavum, both collected from Neotropical region, in the Vale do Catimbau National Park, Brazil, are proposed as new to science. Detailed macro- and micromorphological descriptions, including scanning electron microscopy of the basidiospores, are provided. ITS nrDNA sequence analyses were used to investigate the phylogenetic position of these taxa in the genus Tulostoma. Discussions about related species were carried out.

Putative Photosensitivity In Internal Light Organs (Organs Of Pesta) Of Deep-Sea Sergestid Shrimps, Tamara Frank, Jamie Sickles, Danielle M. Deleo, Patricia Blackwelder, Heather Bracken-Grissom

Marine & Environmental Sciences Faculty Articles

Many marine species can regulate the intensity of bioluminescence from their ventral photophores in order to counterilluminate, a camoufage technique whereby animals closely match the intensity of the downwelling illumination blocked by their bodies, thereby hiding their silhouettes. Recent studies on autogenic cuticular photophores in deep-sea shrimps indicate that the photophores themselves are light sensitive. Here, our results suggest photosensitivity in a second type of autogenic photophore, the internal organs of Pesta, found in deep-sea sergestid shrimps. Experiments were conducted onboard ship on live specimens, exposing the animals to bright light, which resulted in ultrastructural changes that matched those seen …

Determination Of The Genetic Diversity, Population Structure, And Some Ecological Preferences Of The Endemic Muscari Adilii, Ali̇ Murat Keser, Ahmet Emre Yaprak, Gül Ni̇lhan Tuğ

Turkish Journal of Botany

The population structure and genetic diversity of M. adilii and some edaphic and climatic preferences of the species were investigated. Although the chemical characteristics such as pH, EC, and gypsum were similar for the three subpopulations, the physical qualities and lime content of the soil were different from each other. In terms of bioclimate, the Nallıhan station (Nallıhan Bird Sanctuary) has a lower annual average temperature and precipitation value, and monthly average values during the germination and flowering periods, than the Beypazarı station (Hırkatepe and Çoban Ahmet Fountain). Amplification with 16 ISSR markers produced 377 bands from 84 individuals belonging …

Pollen Morphology Of Some Tanacetum L. (Asteraceae) Taxa And Its Systematic Value, Murat Kurşat, İsmai̇l Çelti̇k, Bi̇rol Başer, Fahretti̇n Özbey, İrfan Emre

Turkish Journal of Botany

Pollen grains of 24 Tanacetum taxa from Türkiye were examined by light (LM) and scanning electron microscopy (SEM). Detailed pollen morphological characteristics are provided for these taxa. According to the 24 analyzed taxa, T. parthenifolium has the smallest pollen grains, and T. aurem var. aurem possesses the largest ones. The basic shape of the pollen grains in most taxa is oblatespheroidal. However, suboblate pollen grains are recorded for T. balsamitoides, T. aurem var. aurem, and T. tomentellum. The polar axis ranges from 16.56 to 26.14 µm, and the equatorial diameter ranges from 17.64 to 30.12 µm in this study. …

A New Scrophularia (Scrophulariaceae) Species Without Staminodes In The Enigmatic ?Canina Clade? From The Western Taurus (Türkiye), Candan Aykurt, Mertcan Gülben, Bariş Özüdoğru, Duygu Sari, İsmai̇l Gökhan Deni̇z, Burçi̇n Çingay

Turkish Journal of Botany

The new species of Scrophularia was discovered among the screes and stony slopes of the Alakır Valley (Antalya) located in the Western Taurus. The most remarkable morphological feature of the species is its flowers without staminodes. With this feature, the species is unique among the representatives of the ?Canina? clade in Türkiye. The new species is closely related to S. floribunda endemic to Türkiye and East Aegean Islands (Greece) according to our phylogenetic analyses based on the nuclear ITS and chloroplast trnL-F regions. Apart from its corollas without staminodes, the new species differs from its morphologically similar …

Nuclear Dna Content And Ploidy Levels Of Living Apple Germplasm Collection In Türkiye, Emel Kaçal, Yaren İpek Şi̇mşek, Turgay Seymen, Şeri̇f Özongun, Meti̇n Tuna

Turkish Journal of Botany

One of the key uses of flow cytometry is the estimation of nuclear DNA content and ploidy level, which has proven a reliable and effective approach in many studies. The primary goal of this research is to estimate the nuclear DNA content and ploidy level of the apple genetic resource collection maintained in the Fruit Research Institute?s living Malus collection for the first time using flow cytometry (for autochthonous varieties). Fresh apple leaf tissues were used for the flow cytometry analysis. For each genotype, nuclear DNA analysis was performed on three individual plants. Propidium iodide (PI) is used as a …

Increasing Temperatures Can Pose An Opportunity To Recover Endemic And Endangered Oriental Sweetgum Tree (Liquidambar Orientalis Mill.) From Extinction, Okan Ürker, Nurbahar Usta Baykal, Eren Ada

Turkish Journal of Botany

Evidence suggests that past climatic oscillations caused many species to drastically change their distribution range and had significant impacts on their survival capabilities. There is significant evidence suggesting that today's changing climate threatens many species to face a rapid extinction period in the coming decades and centuries. Understanding the changing range patterns provides significant input on biodiversity and conservation biology studies. However, warming climatic conditions may also present an opportunity for some species to expand their habitats?particularly those adapted to warmer environments. In this study, we asked how near-future climate change will affect the distribution range of the oriental sweetgum …

Identification Of The Functional Domain Of The Dense Core Vesicle Biogenesis Factor Hid-1, Blake H. Hummer, Theodore Carter, Breanna L. Sellers, Jenna D. Triplett, Cedric S. Asensio

Biological Sciences: Faculty Scholarship

Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. HID-1 is a trans-Golgi network (TGN) localized peripheral membrane protein contributing to LDCV formation. There is no information about HID-1 structure or domain architecture, and thus it remains unknown how HID-1 binds to the TGN and performs its function. We report that the N-terminus of HID-1 mediates membrane binding through a myristoyl group with a polybasic amino acid patch but lacks specificity for the TGN. In addition, we show that the C-terminus serves as the functional domain. Indeed, this isolated domain, when tethered to the TGN, …

Evaluation Of Phytochemicals And The Role Of Oxidative Stress Pathways During Fruit Development In Strawberries (Fragaria×Ananassa), Fahimeh Mokhtari Shojaee, Elham Mohajel Kazemi, Houshang Nosrati, Maryam Kolahi, Mehdi Haghi

Turkish Journal of Botany

Strawberries (Fragaria × ananassa) are one of the Rosaceae family, considered economically and nutritionally important berry fruits. Phytochemicals are crucial ingredients that contribute to characteristics such as the fruit?s aroma and flavor. The current study investigated the phytochemical characteristics of strawberry fruit at three developmental stages (green, white, and red). HPLC measurements determined the glucose, fructose, and sucrose concentrations significantly increased in the red fruit, whereas glucose was identified as the major sugar in ripe fruit (2.8 g.g-1FW). Evaluation of the phenylpropanoid pathway implied that the amount of total phenol and flavonoid following phenylalanine ammonia-lyase enzyme activity decreased …

Tmem27 Suppresses Tumor Development By Promoting Ret Ubiquitination, Positioning, And Degradation, Gabriela Huelgas-Morales, Jonathan Lefkowitz, Wan Song, Bethany N Landry, Hector Lopez, Cynthia M Estrada-Zuniga, Shivi Goyal, Mohammad Aasif Khan, Timothy J Walker, Exing Wang, Faqian Li, Yanli Ding, Lois M Mulligan, Ricardo C T Aguiar, Patricia L M Dahia

Faculty, Staff and Student Publications

The TMEM127 gene encodes a transmembrane protein of poorly known function that is mutated in pheochromocytomas, neural crest-derived tumors of adrenomedullary cells. Here, we report that, at single-nucleus resolution, TMEM127-mutant tumors share precursor cells and transcription regulatory elements with pheochromocytomas carrying mutations of the tyrosine kinase receptor RET. Additionally, TMEM127-mutant pheochromocytomas, human cells, and mouse knockout models of TMEM127 accumulate RET and increase its signaling. TMEM127 contributes to RET cellular positioning, trafficking, and lysosome-mediated degradation. Mechanistically, TMEM127 binds to RET and recruits the NEDD4 E3 ubiquitin ligase for RET ubiquitination and degradation via TMEM127 C-terminal PxxY motifs. Lastly, increased cell …

Revisiting The Master Food Volunteer Program: Examining How To Enhance Nutrition Education In The United States, Stacey Viera, Lindsey Haynes-Maslow

Journal of Extension

America’s diet-related illness crisis intersects with a lack of nutrition literacy, nutrition security, and systemic inequities. The Cooperative Extension Service’s (CES) national infrastructure could potentially provide equitable access to quality nutrition education in the US utilizing a Master Food Volunteer (MFV) model. This research brief examined preliminary evidence for the MFV model as a support for CES agents and paraprofessionals, and results show a paucity of evidence. Further research and a pilot program with pre-established measures for health-related knowledge and behaviors could elucidate the model’s potential to increase equitable access to evidence-based programming, nutrition, and implementation guidance.

A Peptide-Binding Domain Shared With An Antarctic Bacterium Facilitates, Cameron J Lloyd, Shuaiqi Guo, Brett Kinrade, Hossein Zahiri, Robert Eves, Fitnat Yildiz, Peter L Davies, Karl E Klose

Faculty, Staff and Student Publications

No abstract provided.