Life Sciences Commons^™

Immunepotent Crp Enhances Cyclophosphamide-Induced Cytotoxicity Through A Caspase Independent But Ros Dependent Mechanism In Triple Negative-Breast Cancer Cells, Ana L. Rivera, A. C. Martínez-Torres, C. Rodríguez-Padilla

Research Symposium

Background: Breast cancer (BC) is one of the leading causes of cancer death worldwide. Cyclophosphamide (CYP) remains a mainstay in cancer therapy mainly in the triple negative breast cancer subtype (TNBC) in spite of harmful adverse effects and cell death-resistances. To face this, combination of chemotherapies and immunotherapies has been proposed. IMMUNEPOTENT CRP (ICRP) is an immunotherapy that has cytotoxic effects in several cancer cells without affecting peripheral blood mononuclear cells (PBMC) and CD3+ cells, beside improving clinical parameters of chemotherapy-treated patients. The aim of this study was to evaluate the mechanism of cytotoxicity induced by ICRP in combination with …

Human Ipsc Derived Cardiomyocyte Model Reveals The Transcriptomic Bases Of Covid-19 Associated Myocardial Injury, Kashish Kumar, Satish Kumar, Erica De Leon, Joanne E. Curran, Sarah Williams-Blangero, John Blangero

Research Symposium

Background: Multi-organ complications have been the hallmark of severe COVID-19; cardiac injuries were reported in 20% to 30% of hospitalized COVID-19 patients, although the disease etiology remains poorly understood. This study leveraged genome-wide RNA-sequence data generated using induced pluripotent stem cell (iPSC) differentiated cardiomyocytes (CMs) and in vitro modeling of SARS-CoV-2 infection in CMs, to understand the molecular mechanisms of COVID-19 myocardial injuries for novel diagnostic and therapeutic development.

Methods: Raw RNA-sequence data sets, GSE165242 and GSE150392 were aligned to human genome assembly GRCh38 and gene expressions were quantified. Differentially expressed (DE) genes between experimental groups were identified using moderated …

Hpv Imprints In Western India: The Overlooked Criteria For Cancer Profiling, Ashi R. Thobias, Jayendra B. Patel, Prabhudas P. Patel

Research Symposium

Background: In India, HPV infection detection for cancer-typing has been largely evaded. Especially, data on prevalence of HPV types other than the highly prevalent HPV 16 and 18 are lacking, particularly from the western region. Thus, present study aimed to evaluate prevalence of HPV strains in three most prevailing cancers in India i.e. cervical, oral and oropharyngeal cancer.

Materials & methods: DNA was isolated from tissue samples of 400 cervical cancer cases, 127 oral cancer cases and 75 oropharyngeal cancer cases and endpoint PCR was performed using degenerative primers MY 09/11, GP 5+/6+ and CP I/II. TS-PCR was conducted to …

Gene-By-Environment Expression And Calculation Of The Frailty Index, Eron G. Manuosv, Vincent P. Diego, John Blangero, Michael C. Mahaney, Sarah Williams-Blangero

Research Symposium

Background: Frailty can be described as a phenotype (e.g., sarcopenia, reduced grip strength, decreased VO2 max) or as a ratio of deficits, i.e., a Frailty Index (FI). FI predicts survival, death, cognitive impairment, falls, and hospitalizations. Frailty is influenced by both genes and environment. We calculated the FI as the sum of measured deficits divided by the total number of items assessed in a pedigree-based sample of 1,029 Mexican Americans participants in the San Antonio Family Heart Study. We performed a novel search for genotype-by-environment interactions (GXE) influencing FI. Such interactions lead to heritable differences between individuals in their responses …

Engineered Exosomes For The Multimodal Imaging Directed Photo-Immunotherapy Of Colorectal Cancer, Deepak S. Chauhan, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu

Research Symposium

Background: Rio Grande Valley experience severe cancer health disparity. A novel therapeutic modality may serve as better therapeutic option. Nanohybrids endowed with multifunctionality, longer circulation time, large surface area have emerged as an active preference for cancer research. However, rising concern of nanomaterials toxicity and scalability issues has slowed their translation to clinics. Exosomes (Exo) are endogenous endocytic origin 40-100 nm vesicles found in various body fluids, which in comparison to synthetic nanoparticles, are biodegradable, highly biocompatible as well as immunocompatible in nature. Although bulk isolation of exosomes from human body fluids is still a problem and engineering of exosomes …

Epoxyazadiradione Exhibit Anti‑Cancer Activities By Modulating Lncrnas Expression In Pancreatic Cancer, Vipin Rai, Nikee Awasthee, Sumit Singh Verma, Subash Chandra Gupta

Research Symposium

Background: Azadirachta indica (neem), a medicinal plant under Meliaceae family, is found in the Indian subcontinent. One of the limonoids, epoxyazadiradione (EPA), is a phytochemical isolated from the seeds of this tree. This is widely used in traditional medicine to treat a variety of human ailments. Although EPA has shown promise against some cancer types, its efficacy against pancreatic cancer and the underlying mechanism remains elusive.

Aim: We examined the anti‑cancer activity of EPA against pancreatic cancer cells. We also examined the underlying mechanism.

Methods: Pancreatic cancer cell lines (PANC-1 and MiaPaCa-2) were used during the study. We performed MTT …

Effect Of Igf-1 On Post-Translational Modifications (Ptms) On A Model Of Diabetes-Induced Cardiac Hypertrophy In H9c2 Cardiomyoblast Cell Line, Andres Medina, Lizbeth Trevino, Alejandra Salinas, Yadira Chaires, Erick Rodriguez

Research Symposium

Background: Diabetic cardiomyocytes alter their post-translational modification levels, especially in OGlcNAcylation and Phosphorylation. Insulin-like growth factor 1 (IGF-1) is a peptide known to induce favorable cardiovascular effects in patients with heart failure. Here, we focus on the downstream effects of IGF-1 as a potential DCM treatment.

Methods: H9c2 cells were cultured in DMEM-10% FBS at 80% of confluence. As a cellular model of cardiac hypertrophy, we used a high-glucose medium (30 mM glucose) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used cells cultured in low-glucose DMEM (glucose 5mM) in the …

Cyclophosphamide And Epirubicin Induce Apoptotic Cell Death In Microglia Cells, Rafael De La Hoz-Camacho

Research Symposium

Background. Chemotherapy Related Cognitive Impairment’s (CRCI), diminish patient’s quality life, being breast cancer (BC) patients the most affected. Microglia is described to play a major role in CRCI; hence, the aim of this research was to describe the cytotoxicity of cyclophosphamide (CTX) and Epirubicin (EPI), on microglia (SIM-A9), compared to BC cells (4T1).

Methods. We assessed cell viability (Resazurin) and cell death (AnnV), as well as nuclear damage with γ-H2AX, p53, p16 and cell cycle analysis (PI staining) by flow cytometry (FC). Furthermore, we evaluated ΔΨm (DIOC6), ROS (DCFDA) and NO (DAF-FM) production. Finally, caspase activation (TF2-VAD-FMK) and autophagy (CYTO-ID). …

Whole Genome Sequence Data Implicate Rbfox1 In Epilepsy Risk In Baboons, Mark Z. Kos, Melanie A. Carless, Lucy Blondell, Mary M. Leland, Koyle D. Knape, Harald H. H. Goring, Charles A. Szabo

Research Symposium

Background: Baboons exhibit a genetic generalized epilepsy (GGE) that resembles juvenile myoclonic epilepsy and may represent a suitable genetic model for human epilepsy. The genetic underpinnings of epilepsy were investigated in a baboon colony at the Southwest National Primate Research Center (San Antonio, TX) through the analysis of whole-genome sequence (WGS) data.

Methods: Baboon WGS data were obtained for 38 cases and 19 healthy controls from the NCBI Sequence Read Archive and, after standard QC filtering, two subsets of variants were examined: (1) 20,881 SNPs from baboon homologs of 19 candidate GGE genes; and (2) 36,169 protein-altering SNPs. Association tests …

Cucurbitacin D Ameliorates Benzo[A]Pyrene Induced Liver Injury Via Activation Of Nrf2 Antioxidant Pathway, Anyssa Rodriguez, Mohammed Sikander, Shabnam Malik, Fathi T. Halaweish, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Background: Co-morbidity variables, such as smoking, are strongly linked to the development and progression of liver cancer. Further, benzo[a]pyrene, a major component of tobacco smoke, is highly carcinogenic and triggers liver damage. Cucurbitacin, a kind of triterpene, has a wide range of biological properties, including antioxidant, anti-inflammatory, and anti-cancer effects. However, the precise mechanism of its hepatoprotective effects is obscure.

Objective: The aim of this study is to investigate the cytoprotective effects of novel analog of cucurbitacin, cucurbitacin D, against benzo[a]pyrene-induced liver injury in HepG2 cells. Method: The cytoprotective efficacy of cucurbitacin D against benzo[a]pyrene-induced liver damage was …

Antp Transcriptional Activity Is Modulated By The Formation Of The Trimeric Antp-Tbp Complexes With Tfiieβ, Exd And Bip2, Norma C. Hernández Bautista, Gustavo Jiménez Mejía, Claudia Altamirano Torres, Diana Reséndez Pérez

Research Symposium

Homeoproteins are transcriptional factors that bind to DNA through a highly conserved binding domain known as the homeodomain (HD) which recognizes short regions rich in AT to control the development of the body appendages of organisms. However, their structural and recognition similarities make it difficult to explain how homeoproteins are capable of carrying out their function. Previous results have shown that Antp homeoprotein can establish dimeric interactions with TBP, TFIIEβ, Exd, BIP2 and more recently through BiFC-FRET we confirmed that Antp and TBP can form trimeric complexes with TFIIEβ/Exd/BIP2. Therefore, is important to show how these trimeric complexes modulate Antp …

Antibody Mediated Targeted Drug Delivery System To Improve Immunotherapy In Pancreatic Cancer, Poornima Devi Shaji, Meena Jaggi, Murali M. Yallapu, Subhash C. Chauhan, Sheema Khan

Research Symposium

About 95% of tumor arises from epithelial cell lining ducts known to be pancreatic ductal adenocarcinomas, with less than 5-7% survival rate. Unfortunately, little progress has been seen in the outcomes of patients with PDAC as tumor develops high desmoplasia and chemo-resistance to chemotherapeutic drugs, such as gemcitabine (Gem). Immunotherapy has shown promising results in cancers, except pancreatic cancer due to their characteristic fibrotic tumor microenvironment. The therapies are unable to penetrate to the fibrotic tumors leading to insufficient availability of the therapeutic drugs at the tumor site. A recently identified mucin, MUC13 is aberrantly expressed in pancreatic tumors but …

A Natural Near-Infrared Fluorescent Probe For Cancer Cell Imaging, Benilde E. Adriano, Nycol M. Cotto, Neeraj Chauhan, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu

Research Symposium

Background: Rio Grande Valley (RGV) suffers from a high prevalence of certain cancers and lack the resources for accurate early diagnosis. Near-infrared (NIR) fluorescence-based imaging is a noteworthy and safer strategy for cancer detection compared to radiological imaging. There are several NIR dyes including indocyanine green (ICG) and its analogues that allow high-resolution and deep tissue imaging. However, these dyes possess some drawbacks, namely photo instability, toxicity, poor water solubility, and short half-lives. Chlorophyll (Chl) is a natural dietary and biocompatible NIR fluorescent substance which has the potential to serve as a cancer NIR imaging candidate. Hence, we aim to …

Exploration Of Potential Natural Inhibitors Against Kras-G12d In Pancan: Protein Centered Pharmacophore Htvs Approach., Anupam Dhasmana, Swati Dhasmana, Sudhir Kotnala, Vivek Kumar Kashyap, Sheema Khan, Murali M. Yallapu, Meena Jaggi, Subhash C. Chuahan

Research Symposium

Background: As per key statistics of American Cancer Society 2021, Pancreatic Cancer (PanCan) affects around 60,430 persons a year in the U.S. and is tricky to diagnose & treat. Studies revealed that African Americans have a 50–90% higher incidence of PanCan compared to other ethnic groups. Oncogenic KRAS mutation is the signature genetic incident in the progression and development of PDAC. KRAS is the most common protein which is 95% times mutated in PDAC condition. By considering this alarming situation our group is now focused on to develop therapeutic portfolio against KRAS-G12D mutation associated PanCan by using high through-put virtual …

Mortaparibplus- A Novel Anticancer Small Molecule Abrogating Mortalin-P53 Interaction In Cancer Cells, Anissa N. Sari, Ahmed Elwakeel, Jaspreet K. Dhanjal, Vipul Kumar, Durai Sundar, Sunil C. Kaul, Renu Wadhwa

Research Symposium

Background. The cessation of tumor cell growth through cell cycle arrest and apoptosis is determined by p53, a tumor suppressor protein. However, the interaction between mortalin-p53 within cytoplasm/nucleus leads to the inactivation of p53 transcriptional activation function. The disruption of mortalin-p53 complex has been suggested as an approach for developing a potential anticancer drug.

Methods. A screening of a high-content chemical library was performed to determine a molecule with mortalin-p53-interaction disrupting characteristics. After four-rounds of visual assays, we discovered a triazole derivative (4-[(1E)-2-(2-phenylindol-3-yl)-1-azavinyl]-1,2,4-triazole, named Mortaparib^Plus) with a potential ability of disrupting mortalin-p53-complex. In this study, we recruited …

Systems Network Analysis Of Protein Interaction Network (Pin) For Deducing Molecular Mechanistic Action Of Bap Induced Carcinogenesis, Anukriti ., Anupam Dhasmana, Uma Bhardwaj

Research Symposium

Background: Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, has been placed in group 1 by IARC which indicates that it is a potential carcinogen to human beings. It has shown tumorigenic properties in approximately all animal model systems. In the current study, we have tried to identify the most probable biomolecular targets of BaP using systems biology approach.

Method: All the proteins that interact with BaP were extracted from T3DB. STRING-db was used to generate the Protein- protein interaction network (PPIN). Various apps of cytoscape software were used for network analysis, modulation and GO enrichment analysis. By developing biokinetic …

Gamma-Aminobutyric Acid In The Midbrain: Role In Sensation, Perception, And Neurological Disorders, Truc Le, Cristian Botello, John Vandeberg, Mario Gil

Research Symposium

Background: Altered GABA expression in the midbrain is associated with neurological conditions like epilepsy and Parkinson’s Disease. In the genetically epilepsy prone rat, an increase in GABA neurons in the inferior colliculus increases seizure susceptibility. In contrast, Parkinson’s Disease is associated with altered GABA expression in the substantia nigra. The purpose of this study is to identify patterns between the sensory and motor regions of the midbrain and to quantify a baseline of GABA neurons in these areas.

Methods: The Allen Institute’s reference atlas of the mouse and BrainMap.org was used to identify the sensory and motor areas of the …

The Santa Clara, 2023-09-29, Santa Clara University

The Santa Clara

No abstract provided.

Effects Of Cortisol, Epinephrine, And Bisphenol Contaminants On The Transcriptional Landscape Of Marine Mammal Blubber, Lauren Kashiwabara, Laura Pirard, Cathy Debier, Daniel Crocker, Jane Khudyakov

Pacific Faculty Work

Top ocean predators such as marine mammals are threatened by intensifying anthropogenic activity, and understanding the combined effects of multiple stressors on their physiology is critical for conservation efforts. We investigated potential interactions between stress hormones and bisphenol contaminants in a model marine mammal, the northern elephant seal (NES). We exposed precision-cut adipose tissue slices (PCATS) from blubber of weaned NES pups to cortisol (CORT), epinephrine (EPI), bisphenol A (BPA), bisphenol S (BPS), or their combinations (CORT-EPI, BPA-EPI, and BPS-EPI) ex vivo and identified hundreds of genes that were differentially regulated in response to these treatments. CORT altered expression of …

The Pepsis Menechma Lepeletier (Hymenoptera: Pompilidae: Pepsinae) Taxonomic And Nomenclatural Problem, Frank E. Kurczewski

Insecta Mundi

Hurd (1952), in revising the Nearctic species of Pepsis Fabricius, separated P. cerberus Lucas from P. elegans Lepeletier based on external morphology and geography. Vardy (2005), in his Western Hemisphere Pepsis revision, combined these taxa and several Neotropical color and structural variants in a broad defini­tion of P. menechma Lepeletier extending across ~11,250 km and two continents. Vardy (2005) synonymized the familiar and well-documented, 160-year-old P. elegans under P. menechma probably because it appeared several pages later in Lepeletier’s (1845) Histoire Naturelle des Insectes. Hyménoptères. Vardy’s (2005) interpre­tation of Pepsis menechma as a viable species presents a taxonomic and …

Establishment Of Sinoxylon Anale Lesne (Coleoptera: Bostrichidae) In Brazil And Its Potential Implications, Luana De Souza Covre, Robert A. Hack, Carlos A.H. Flechtmann

Insecta Mundi

Bostrichidae (Coleoptera) are a family of beetles with specialized xylophagous feeding habits that allow consumption of dry woody plant tissues. Bostrichids are often polyphagous and therefore pose a sig­nificant threat to many agricultural and forestry products, particularly in tropical regions. Bostrichids are commonly detected in solid wood packaging material at international ports of entry. Notably, Sinoxylon anale Lesne has been intercepted in wood crates and pallets worldwide and has now become established in Brazil. This paper reports the first documented establishment of S. anale in Brazil, being found both in domestic wood pallets and within native forest and monoculture. The …

Homoeopathic Medicines Modulate Inflammatory Functions And Adhesion Receptor Expression In Human Blood Cells: An In Vitro Study, Nibedita Dalpati, Shubham Kumar Rai, Divya Singh, Shiba Prasad Dash, Sukanti S. Sarangi, Mahabahu Nayak, Pranita P. Sarangi

Indian Journal of Research in Homoeopathy

Background: Homoeopathic medicines are used extensively for the treatment of many human diseases and are shown to affect immune cell functions. However, our understanding of the immunomodulatory effects of such medicines and associated mechanisms of action remains limited. Objectives: The present study aims to investigate the immunomodulatory effects of Arsenic album, Rhus toxicodendron, Hepar sulphuris and Bryonia alba on lipopolysaccharide (LPS) induced peripheral blood neutrophils and monocytes. Materials and Methods: In this work, neutrophils and monocytes were treated with different homoeopathic dilutions of 30C potency separately, followed by stimulation with the LPS, to examine the immunomodulatory effects of these …

A Case Report: A Patient Rescued By Va-Ecmo After Cardiac Arrest Triggered By Trigeminocardiac Reflex After Nasal Surgery, Liwei Wang

Faculty, Staff and Student Publications

Rationale:

Cardiac arrest (CA) caused by trigeminocardiac reflex (TCR) after endoscopic nasal surgery is rare. Hence, when a patient suffers from TCR induced CA in the recovery room, most doctors may not be able to find the cause in a short time, and standard cardiopulmonary resuscitation and resuscitation measures may not be effective. Providing circulatory assistance through venous-arterial extracorporeal membrane oxygenation (VA-ECMO) can help healthcare providers gain time to identify the etiology and initiate symptom-specific treatment.

Patient concerns:

We report a rare case of CA after endoscopic nasal surgery treated with VA-ECMO.

Diagnoses:

We excluded myocardial infarction, pulmonary embolism, allergies, …

Smokejumper Obituary: Lowden, Robert G. (Cave Junction 1967), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Chinese Species Of The Genus Tricondyla Latreille, 1822 (Coleoptera: Cicindelidae) And A New Record For The Country, Xinxin Xiong, Jürgen Wiesner

Insecta Mundi

Tricondyla wiesneri Naviaux, 2002 (Coleoptera: Cicindelidae) is reported from China for the first time. Photographs, short descriptions, and an identification key for all six taxa of the genus Tricondyla La­treille, 1822 known from China are given.

The first author recently received several specimens of Tricondyla wiesneri Naviaux, 2002 that had been collected in Yingjiang County, Dehong Dai and Jingpo Autonomous Prefecture, Yunnan Province, China. The second author compared them with the type specimens and confirmed the identification. The species was originally described from Laos, Louang Namtha province (Naviaux 2002: 48), later reported from Vietnam, Dien Bien province (Wiesner 2022: 191), …

First Report Of The Stinkbug Edessa Leucogramma (Perty) (Hemiptera: Heteroptera: Pentatomidae: Edessinae) Attacking Handroanthus Chrysanthus (Jacq.) S.O. Grose (Bignoniaceae), With Descriptions Of The Adult And Immatures And Notes On Associated Fungi And Protozoa, Gonzalo Abril R., Allan H. Smith-Pardo

Insecta Mundi

The stinkbug Edessa leucogramma (Perty) (Hemiptera: Pentatomidae: Edessinae) is reported as a pest of young yellow guayacán trees (Handroanthus chrysanthus (Jacq.) S.O. Grose, Bignoniaceae) in the met­ropolitan area of the Aburra Valley in Antioquia, Colombia (AMVA). We provide a short description of the adult and immature stages and report for the first-time protozoa associated with the digestive system of this species of true bug in addition to information regarding a fungus found associated with Edessa leucogramma in the field.

Se reporta el chinche Edessa leucograma (Perty) (Hemíptera: Pentatomidae: Edessinae), como plaga importante de árboles jóvenes del guayacán amarillo ( …

Cyclocephala Kuijteni (Scarabaeidae: Dynastinae: Cyclocephalini), A New Species From Suriname, Meindert A. Hielkema

Insecta Mundi

Cyclocephala kuijteni, new species (Scarabaeidae: Dynastinae: Cyclocephalini), is described from Suriname. It is illustrated together with its aedeagus, and the characteristics differentiating it from the most similar species C. castanea (Olivier), C. hardyi Endrödi and C. pygidialis Joly are briefly discussed.

The New World genus Cyclocephala Dejean, 1821 contains about 360 described species, with most representatives occurring in the Neotropics (Ratcliffe and Cave 2015). While investigating the scarabaeoid fauna of Suriname with my son Auke, we collected a large number of Cyclocephala specimens with a so-called mothing sheet, including a single male specimen I was unable to identify. The …

Heterogenous Lung Inflammation Ct Patterns Distinguish Pneumonia And Immune Checkpoint Inhibitor Pneumonitis And Complement Blood Biomarkers In Acute Myeloid Leukemia: Proof Of Concept, Muhammad Aminu, Naval Daver, Myrna C B Godoy, Girish Shroff, Carol Wu, Alberto Goizueta, Saadia A Faiz, Mehmet Altan, Guillermo Garcia-Manero, Hagop Kantarjian, Farhad Ravandi-Kashani, Tapan Kadia, Marina Konopleva, Courtney Dinardo, Sherry Pierce, Aung Naing, Sang T Kim, Dimitrios P Kontoyiannis, Fareed Khawaja, Caroline Chung, Jia Wu, Ajay Sheshadri

Faculty, Staff and Student Publications

BACKGROUND: Immune checkpoint inhibitors (ICI) may cause pneumonitis, resulting in potentially fatal lung inflammation. However, distinguishing pneumonitis from pneumonia is time-consuming and challenging. To fill this gap, we build an image-based tool, and further evaluate it clinically alongside relevant blood biomarkers.

MATERIALS AND METHODS: We studied CT images from 97 patients with pneumonia and 29 patients with pneumonitis from acute myeloid leukemia treated with ICIs. We developed a CT-derived signature using a habitat imaging algorithm, whereby infected lungs are segregated into clusters ("habitats"). We validated the model and compared it with a clinical-blood model to determine whether imaging can add …

Elastic Stable Intramedullary Nail Fixation Versus Submuscular Plate Fixation Of Pediatric Femur Shaft Fractures In School Age Patients: A Prisma-Compliant Systematic Review And Meta-Analysis, Donglai Hu, Zihang Xu, Tao Shi, Hui Zhong, Yuting Xie

Faculty, Staff and Student Publications

BACKGROUND: Studies of clinical outcomes that compare the elastic stable intramedullary nail (ESIN) with the submuscular plate (SMP) were controversial. The meta-analysis was performed to summarize existing evidence, aiming to determine whether ESIN was superior to SMP in pediatric femur shaft fractures.

METHODS: Search strategies followed the recommendations of the Cochrane collaboration. Electronic searches such as PubMed, Embase, Web of Science, Cochrane were systematically searched for publications concerning ESIN and SMP from the inception date to March 2023. Two investigators independently searched, screened, and reviewed the full text of the article. Disagreements generated throughout the process were resolved by consensus, …

New Jak3-Insl3 Fusion Transcript-An Oncogenic Event In Cutaneous T-Cell Lymphoma, Loka Reddy Velatooru, Cheng Hui Hu, Pedram Bijani, Xiaohong Wang, Pierr Bojaxhi, Madeleine Duvic

Faculty, Staff and Student Publications

Constitutively activated tyrosine kinase JAK3 is implicated in the pathogenesis of cutaneous T-cell lymphomas (CTCL). The mechanisms of constitutive JAK3 activation are unknown although a JAK3 mutation was reported in a small portion of CTCL patients. In this study, we assessed the oncogenic roles of a newly identified JAK3-INSL3 fusion transcript in CTCL. Total RNA from malignant T-cells in 33 patients with Sézary syndrome (SS), a leukemic form of CTCL, was examined for the new JAK3-INSL3 fusion transcript by RT-PCR followed by Sanger sequencing. The expression levels were assessed by qPCR and correlated with patient survivals. Knockdown and/or knockout assays …