Life Sciences Commons^™

Freshwater Macroinvertebrate Communities In Baldwin County, Georgia, Heath Michael Ghioto, Michelle K. Murdock, Nadya S. Gutierrez, Kristine N. White Ph.D.

Georgia Journal of Science

Freshwater ecosystems are critical habitats for maintaining biodiversity, often providing refuge for organisms especially in urban settings. Baldwin County, GA is home to many freshwater lakes that are part of the Oconee River watershed. Despite ongoing water quality monitoring, aquatic macroinvertebrates are under studied in the area. Aquatic macroinvertebrate diversity of one forested and one residential lake in Milledgeville, GA was documented for the first time. Despite low sample size, community composition was significantly different between lakes, with 27 families in Lake Laurel (forested), 44 families in Lake Oliver Hardy (residential), and only 19 families collected from both lakes. Seasonal …

An Investigation Of Mitigation Measures On The Spread Of Covid-19 In A College Classroom Using Agent-Based Modeling, Saharsh Talwar, Anne E. Yust

Spora: A Journal of Biomathematics

In this manuscript, we describe the process of using agent-based modeling in NetLogo to create a simulation of COVID-19 spread in a traditional college classroom. The model allows for an evaluation of different preventative measures implemented by the University of Pittsburgh, including the cohort classroom attendance model, mask and vaccine mandates, contact tracing, and classroom sanitation. Through the use of the model's interactive interface, the impact of adjusting specific measures by the institution could be visualized, providing a valuable tool for combating diseases that spread through droplet transmission.

2023 Celebration Of Scholarship, Creativity, And Engagement, Todd Bruns, Diane Highland

Programs

Annually, Eastern Illinois University honors faculty achievements in the areas of research, creativity, and engagement. This year, the list of faculty accomplishments is again truly impressive, reflective of an engaged faculty committed to excellence and the ongoing pursuit and creation of knowledge. A number of these accomplishments occurred in collaboration with our students, who serve as co-authors, research assistants and partners in bringing these activities to fruition. Indeed, in some cases these professional ambitions transpired over the course of many years, and are a testament to persistence and tenacity--ensuring that these works extend to a broader community of scholars that …

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the United States Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, …

Opportunities For Maximizing The Dietary Quality Of Fad Diets, Jessica M. Phelan, Jillian M. Joyce, Katherine Bode, Sara K. Rosenkranz

Kinesiology and Nutrition Sciences Faculty Research

The quality of American diets, measured by the Healthy Eating Index (HEI), has remained stable and low since 2005. The Dietary Guidelines for Americans 2020–2025 call for research analyzing dietary patterns to determine how guidelines might be altered to increase healthy eating. The present paper seeks to determine the dietary quality of popular fad dietary patterns among Americans. A definition of “fad diet” was created, and Google Trends© was searched for popular diets to determine popular dietary patterns based on the fad diet definition. Finally, eight dietary patterns were identified for inclusion. One-week sample menus were created for each dietary …

Oregon State Rank Assessment For Blue-Gray Gnatcatcher (Polioptila Caerulea), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Blue-gray gnatcatcher (Polioptila caerulea) using NatureServe methodology, 2023.

Diesel Tractor Fuel Efficiency And Exhaust Emissions Standards, Jerin Tekolste, Cory Walters, Michael Mccullough, Lynn Hamilton, Lia Nogueira, Roger M. Hoy

Cornhusker Economics

Diesel engine performance and costs represent crucial factors for agricultural producers while pollutants from the exhaust are largely a social concern but also important to producers. Beginning in 1970, Congress authorized the Environmental Protection Agency to regulate emissions with amendments in subsequent years (U.S. EPA, 2023). In 1996 the EPA issued strict Exhaust Emission Standards for Nonroad Compression-Ignition Engines, causing a major paradigm shift in acceptable emission levels. A primary concern coming from many manufacturers was the difficulty of designing an engine to meet these standards without compromising the engines’ power output and efficient fuel consumption (Lloyd and Cackette 2001; …

Oregon State Rank Assessment For Great Basin Spadefoot (Spea Intermontana), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Great Basin spadefoot (Spea intermontana) using NatureServe methodology, 2023.

Oregon State Rank Assessment For Lark Sparrow (Chondestes Grammacus), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Lark sparrow (Chondestes grammacus) using NatureServe methodology, 2023.

Hilling As A Cultural Control Strategy For Soybean Gall Midge (Diptera: Cecidomyiidae), Anthony Justin Mcmechan, Joana Schroeder De Souza, Natasha Umezu, Pragya Gupta, Gabriela Inveninato Carmona

Department of Entomology: Faculty Publications

Soybean gall midge, Resseliella maxima Gagné, was recently identified as a new species causing significant injury to soybean and is currently found in 164 counties across 7 midwestern states (NE, IA, SD, MN, MO, ND, and KS). Infestation of soybean begins in late spring, when adults emerge from last year’s soybean field. Infestation of a new soybean crop depends on the presence of fissures which start to form at the base of the soybean plant around the V2 stage. Field observations indicate that these fissures are only present below the cotyledonary nodes or in the area within 3–5 cm above …

Decoding Meningioma Heterogeneity And Neoplastic Cell-Macrophage Interaction Through Single-Cell Transcriptome Profiling Across Pathological Grades, Hailang Fan, Lairong Song, Jian Fan, Junpeng Ma, Xiaojie Li, Jian Hu, Dake Zhang, Liang Wang

Faculty, Staff and Student Publications

Background: Analyzing meningioma of distinct pathological types at the single-cell level can provide new and valuable insights into the specific biological mechanisms of each cellular subpopulation, as well as their vital interplay within the tumor microenvironment.

Methods: We recruited patients diagnosed with four distinct types of meningioma and performed single-cell RNA sequencing on their tumor samples, concurrently analyzing a publicly available dataset for comparison. Next, we separated the cells into discrete clusters and identified their unique identities. Using pseudotime analysis, we demonstrated cellular differentiation and dynamics. To investigate biological function, we employed weighted gene co-expression network analysis, gene regulatory network, …

Phenotypic Maps For Precision Medicine: A Promising Systems Biology Tool For Assessing Therapy Response And Resistance At A Personalized Level, Sayantan Bhattacharyya, Shafqat F Ehsan, Loukia G Karacosta

Faculty, Staff and Student Publications

In this perspective we discuss how tumor heterogeneity and therapy resistance necessitate a focus on more personalized approaches, prompting a shift toward precision medicine. At the heart of the shift towards personalized medicine, omics-driven systems biology becomes a driving force as it leverages high-throughput technologies and novel bioinformatics tools. These enable the creation of systems-based maps, providing a comprehensive view of individual tumor's functional plasticity. We highlight the innovative PHENOSTAMP program, which leverages high-dimensional data to construct a visually intuitive and user-friendly map. This map was created to encapsulate complex transitional states in cancer cells, such as Epithelial-Mesenchymal Transition (EMT) …

Workshop Abstracts: The International Conference & Workshop On Lobster (And Crab) Biology And Management, Department Of Primary Industries And Regional Development, Western Australia, Western Rock Lobster Council, Fisheries Research And Development Corporation

International Conference on Lobster (and Crab) Biology and Management (ICWL)

The theme of the 12th ICWL is ‘ecosystem-based fisheries management (EBFM)’. EBFM is a holistic approach to fisheries management that recognises all the interactions within an ecosystem rather than considering a single species or issue in isolation. We consider this represents best practice for fisheries management and reflects that fisheries research and management focus is now broader than just sustainability. Therefore, in addition to presentations that focus on lobster (and crab) biology and stock assessment, we have also welcomed presentations that examine other aspects of EBFM, such as ecosystem effects of fishing, economic assessments, social issues, governance, and compliance with …

The Contractual Liability Arising From The Violation Of Negotiations Through Modern Means Of Communication In The Jordanian Legislation, Yassin Al Qudah, Juhainah Al Momani

This study aims to show the effects of contractual liability for breach of negotiation via electronic means of communication in Jordanian legislation. Where it became clear that preliminary electronic negotiations are a previous basic stage in the electronic contracting process, in terms of organizing the conduct of negotiations by putting forward ideas and offers between the negotiating parties, and discussing among them to reach mutual agreement until the negotiated contract is concluded. As a result, at this stage, a set of obligations is created by each of the parties, otherwise civil liability may arise on the breaching party. This study …

The Effectiveness Of Field Training In Qualifying Students Of Media Faculties For The Labor Market: Yarmouk And Middle East Universities As A Model, Saddam Al-Masshaqbah, Ramez Abuhasirah

The study aimed to identify the effectiveness of the field training course in raising the capabilities of students of media faculties in public and private Jordanian universities and qualifying them for the labor market. The results showed that the degree of students' commitment to attendance during the field training period in media institutions came to a "huge" degree, at a rate of (39.8%), while the degree of their benefit from field training came to a "medium" degree, at a rate of (29.2%). In: "Journalism Editing," Preparation of Radio and Television Programs," and "The Art of Speaking and Presentation." training" with …

Palestinian Journalists' Trends Towards Freelance Platforms As An Alternative Of Traditional Work In Journalistic Work And The Gratifications Achieved "A Field Study", Amin Wafi

This study aims to identify the trends of media students towards the use of digital freelancer platforms in media work as an alternative to traditional journalism by knowing the degree of interest of students in digital freelancer platforms. The degree to which they are exposed، the reasons for exposure، the most important subjects to which they are exposed، the most important platforms they pursue، the most important skill needs، motivations، and stimuli that drive them to deal with freelancer platforms as an alternative to traditional journalism. It is a descriptive study and applied the survey curriculum in the framework of …

Book Review: Australian Caves And Karst Systems, John Webb, Susan White, Garry K. Smith, Jo De Waele

International Journal of Speleology

No abstract provided.

Phenotypic Plasticity Of Male Calls In Two Populations Of The Katydid Neoconocephalus Triops (Insecta: Tettigoniidae), Oliver M. Beckers, Teiya Kijimoto, Johannes Schul

Faculty & Staff Research and Creative Activity

The ability to respond to environmental changes plays a crucial role for coping with environmental stressors related to climate change. Substantial changes in environmental conditions can overcome developmental homeostasis, exposing cryptic genetic variation. The katydid Neoconocephalus triops is a tropical species that extended its range to the more seasonal environment of North America where it has two reproductive generations per year. The harsher winter conditions required adults to diapause which resulted in substantially different mating calls of the diapausing winter animals compared to the non-overwintering summer animals in northern Florida. The summer call corresponds to that of tropical populations, whereas …

Wics Conference 2023: Moving Beyond The Ordinary, Daren Redfearn, Daran Rudnick

Water and Integrated Cropping Systems Conferences

The theme of this year’s WICS Conference is Moving Beyond the Ordinary with the goal of addressing Team Science. Our vision is to provide opportunities and connection points among IANR faculty to leverage their individual knowledge, skills, abilities, and passions. A tendency for land grant institutions is often to deliver information biased from a single, primary discipline and perspective. This is known as comfort zone mega bias and has been defined as “the tendency to drag a problem into our comfort zone and solve the problem that we know how to solve, rather than solving the problem that needs to …

Oregon State Rank Assessment For Redside Shiner (Richardsonius Balteatus), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for redside shiner (Richardsonius balteatus) using NatureServe methodology, 2023.

Changes In Rna Expression Of Hut78 Cells Resulting From The Hiv-1 Viral Protein R R77q Mutation, Joshua S. Ramsey

Theses and Dissertations

Human immunodeficiency virus type 1 (HIV-1) is the causative virus for acquired immunodeficiency syndrome (AIDS). AIDS is characterized by chronic inflammation and reduction of CD4+ T-cells in the blood. This leads to the patient becoming immunocompromised and much more susceptible to disease in general. Different phenotypes in the progression of AIDS have been observed in patients in either progressing to AIDS faster as a Rapid Progressor (RP), or slower as a Long-Term Non-Progressor (LTNP). Researching elements that result in the LTNP phenotype is of interest as it has the potential to offer alternative treatments and therapies to those suffering from …

Population-Level Dna Study Of Stenotrema And Euchemotrema (Gastropoda: Polygyridae: Stenotrematini) Reveals Majority Of Species Are Poorly Circumscribed, Alexander C. Cooper, Elizabeth A. Bergey, Kathryn E. Perez

School of Integrative Biological & Chemical Sciences Faculty Publications

The North American land snail tribe Stenotrematini (Emberton, 1995) is composed of 40 taxa from the genera Stenotrema (Rafinesque, 1819) and Euchemotrema (Archer, 1939). We conducted the first thorough examination of this group since 1948 by sequencing 196 individuals representing 31 of 40 members of the tribe using a combination of cytochrome c oxidase subunit I (COI) and a portion of the 5.8S gene of the large ribosomal subunit (LSU) with the accompanying internal transcribed spacer (ITS-2) region. We reexamined the validity of this tribe by incorporating new sequences with previous DNA work on the …

Structural And Functional Characterizations Of The Two Mini-Ferritins In Pseudomonas Aeruginosa Revealed The Coexistence Of A Classic Dps (Pa0962) And A Dps-Like (Pa4880) Protein, Rajapaksha Pathirannahalage Nimesha Hansani Rajapaksha

LSU Doctoral Dissertations

In the P. aeruginosa genome, gene PA0962 codes for a probable DNA-binding protein from starved cells (Pa Dps). Pa Dps is assembled from 12 monomers, each consisting of a four-helix bundle fold typical of Dps proteins. A subunit dimer of Pa Dps harbors two ferroxidase centers. Assembly of a dodecameric structure of Pa Dps is favored by the presence of divalent metal ions and at pH 6.0 in the absence or presence of divalent cations. Pa Dps is unable to form the large DNA-protein complexes typical of many Dps proteins. Instead, Pa Dps acts on plasmid DNA to form relaxed …

Uracil-Dna Glycosylase Of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently Of Its Catalytic Residues, Kyle R Smith, Somnath Paul, Qiwen Dong, Orchi Anannya, Darby G Oldenburg, Kevin M Mcbride

Faculty, Staff and Student Publications

Herpesviruses are large double-stranded DNA viruses that encode core replication proteins and accessory factors involved in nucleotide metabolism and DNA repair. Mammalian uracil-DNA glycosylases (UNG) excise deleterious uracil residues from their genomic DNA. Each herpesvirus UNG studied to date has demonstrated conservation of the enzymatic function to excise uracil residues from DNA. We previously reported that a murine gammaherpesvirus (MHV68) with a stop codon in ORF46 (ORF46.stop) that encodes for vUNG was defective in lytic replication and latency in vivo. However, a mutant virus that expressed a catalytically inactive vUNG (ORF46.CM) had no replication defect unless coupled with additional …

Myeloablative Fractionated Busulfan For Allogeneic Stem Cell Transplant In Older Patients Or Patients With Comorbidities, Uday R Popat, Oren Pasvolsky, Roland Bassett, Rohtesh S Mehta, Amanda Olson, Amin M Alousi, Qaiser Bashir, Alison M Gulbis, Chitra M Hosing, Jin S Im, Partow Kebriaei, Issa Khouri, David Marin, Yago Nieto, Betul Oran, Neeraj Saini, Terri Lynn Shigle, Samer A Srour, Jeremy L Ramdial, Katayoun Rezvani, Muzaffar H Qazilbash, Borje S Andersson, Richard E Champlin, Elizabeth J Shpall

Faculty, Staff and Student Publications

Traditional conditioning regimens for patients undergoing allogeneic hematopoietic cell transplantation (allo-HCT) provide suboptimal outcomes, especially for older patients and those with comorbidities. We hypothesized that a fractionated myeloablative busulfan dose delivered over an extended period would reduce nonrelapse mortality (NRM) while retaining antileukemic effects. Here, we performed a phase 2 trial for adults with hematological malignancies receiving matched related or unrelated allo-HCT. Participants received busulfan 80 mg/m2 as outpatients on days -20 and -13 before transplant. Fludarabine 40 mg/m2 was administered on days -6 to -3, followed by busulfan dosed to achieve a target area under the curve of 20 …

Somatic Mutational Landscape Of Hereditary Hematopoietic Malignancies Caused By Germline Variants In Runx1, Gata2, And Ddx41, Claire C Homan, Michael W Drazer, Kai Yu, David M Lawrence, Jinghua Feng, Matthew J Pozsgai, Thuong Ha, Parvathy Venugopal, Peer Arts, Sarah L King-Smith, Jesse Cheah, Mark Armstrong, Paul Wang, Csaba Bödör, Alan B Cantor, Mario Cazzola, Erin Degelman, Courtney D Dinardo, Nicolas Duployez, Remi Favier, Stefan Fröhling, Ana Rio-Machin, Jeffery M Klco, Alwin Krämer, Mineo Kurokawa, Joanne Lee, Luca Malcovati, Neil V Morgan, Georges Natsoulis, Carolyn Owen, Keyur P Patel, Claude Preudhomme, Hana Raslova, Hugh Rienhoff, Tim Ripperger, Rachael Schulte, Kiran Tawana, Elvira Velloso, Benedict Yan, Erika Kim, Raman Sood, Amy P Hsu, Steven M Holland, Kerry Phillips, Nicola K Poplawski, Milena Babic, Andrew H Wei, Cecily Forsyth, Helen Mar Fan, Ian D Lewis, Julian Cooney, Rachel Susman, Lucy C Fox, Piers Blombery, Deepak Singhal, Devendra Hiwase, Belinda Phipson, Andreas W Schreiber, Christopher N Hahn, Hamish S Scott, Paul Liu, Lucy A Godley, Anna L Brown, Nisc Comparative Sequencing Program

Faculty, Staff and Student Publications

Individuals with germ line variants associated with hereditary hematopoietic malignancies (HHMs) have a highly variable risk for leukemogenesis. Gaps in our understanding of premalignant states in HHMs have hampered efforts to design effective clinical surveillance programs, provide personalized preemptive treatments, and inform appropriate counseling for patients. We used the largest known comparative international cohort of germline RUNX1, GATA2, or DDX41 variant carriers without and with hematopoietic malignancies (HMs) to identify patterns of genetic drivers that are unique to each HHM syndrome before and after leukemogenesis. These patterns included striking heterogeneity in rates of early-onset clonal hematopoiesis (CH), with a high …

Identification Of Chronic Wasting Disease Prions In Decaying Tongue Tissues From Exhumed White-Tailed Deer, Paulina Soto, Francisca Bravo-Risi, Rebeca Benavente, Stuart Lichtenberg, Mitch Lockwood, Rodrigo Morales

Faculty, Staff and Student Publications

Chronic wasting disease (CWD) prions cause fatal neuropathies in farmed and free-ranging cervids. The deposition of prions in natural and humanmade environmental components has been implicated as a major mechanism mediating CWD spread in wild and captive populations. Prions can be deposited in the environment through excreta, tissues, and carcasses from pre-clinical and clinical animals. Furthermore, burial of CWD-positive animals may reduce but not completely mitigate prion spread from carcasses into the surrounding environment. Here, we analyzed exhumed, decaying deer carcasses for the presence of CWD prions. By analyzing tongue tissues through the protein misfolding cyclic amplification (PMCA) technique, we …

Essential Role Of The Amino-Terminal Region Of Drosha For The Microprocessor Function, Amit Prabhakar, Song Hu, Jin Tang, Prajakta Ghatpande, Giorgio Lagna, Xuan Jiang, Akiko Hata

Biology

Drosha is a core component of the Microprocessor complex that cleaves primary-microRNAs (pri-miRNAs) to generate precursor-miRNA and regulates the expression of ∼80 ribosomal protein (RP) genes. Despite the fact that mutations in the amino-terminal region of Drosha (Drosha-NTR) are associated with a vascular disorder, hereditary hemorrhagic telangiectasia, the precise function of Drosha-NTR remains unclear. By deleting exon 5 from the Drosha gene and generating a Drosha mutant lacking the NTR (ΔN), we demonstrate that ΔN is unable to process pri-miRNAs, which leads to a global miRNA depletion, except for the miR-183/96/182 cluster. We find that Argonaute 2 facilitates the processing …

Genomic Insights Into The Mystery Of Mouse Mummies On The Summits Of Atacama Volcanoes, Jay F. Storz, Schuyler Liphardt, Marcial Quiroga-Carmona, Naim M. Bautista, Juan C. Opazo, Guillermo D’Elía, Jeffrey M. Good

Jay F. Storz Publications

Our understanding of the limits of animal life is continually revised by scientific exploration of extreme environments. Here we report the discovery of mummified cadavers of leaf-eared mice, Phyllotis vaccarum, from the summits of three different Andean volcanoes at elevations 6,029–6,233 m above sea level in the Puna de Atacama in Chile and Argentina. Such extreme elevations were previously assumed to be completely uninhabitable by mammals. In combination with a live-captured specimen of the same species from the nearby summit of Volcán Llullaillaco (6,739 m),¹ the summit mummies represent the highest altitude physical records of mammals in the …

Oregon State Rank Assessment For Prickly Sculpin (Cottus Asper), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Prickly sculpin (Cottus asper) using NatureServe methodology, 2023.