Life Sciences Commons^™

The Effect Of Topical Cannabidiol On The Progression Rate Of Delayed Onset Muscle Soreness, Anastasia Alpy, George Yusuff, Michele Aquino, John Petrizzo, Robert M. Otto, John Wygand, Troy Simpson

International Journal of Exercise Science

International Journal of Exercise Science 16(3): 1426-1439, 2023. This study investigated the efficacy of topical cannabidiol (CBD) ointment in reducing localized inflammation, minimizing performance detriments, and attenuating soreness associated with delayed onset muscle soreness (DOMS). In a double blind randomized control trial, upper-arm circumferences, maximal voluntary isometric contractions (MVICs) for elbow flexion at 90° and 30° for college-aged participants (n = 21, age 20.8 ± 1.9 years) were assessed at baseline. Participants then performed a DOMS-inducing protocol for the biceps brachii. Topical CBD ointment and placebo (P) ointment were randomly assigned and applied 30 minutes, 24, 48 and 72 …

Prediction Of Maximum Lactate Concentration During An All-Out Anaerobic Test In Elite Ice Hockey Players, Maxime Allisse, Hung Tien Bui, Patrick Desjardins, Philippe Roy, Alain Steve Comtois, Mario Leone

International Journal of Exercise Science

International Journal of Exercise Science 16(4) 1385-1397, 2023. The lack of specific on-ice tests to predict maximum lactate concentration limits the ability of coaches to better track and develop their ice hockey players. Thus, this study aimed to develop an equation for indirectly assessing the maximum lactate concentration produced from an all-out on-ice skating effort in elite adolescent ice hockey players. Twenty elite male ice hockey players participated in this study (age = 15.7 ± 1.0 year). The lactate anaerobic skating test (LAST) consisted of skating back and forth on an 18.2 m course at maximal speed with abrupt stops …

The Effects Of Pre-Exercise Ice-Slurry Ingestion On Thermoregulation And Exercise Performance Of Highly Trained Athletes: A Scoping Review, Gopathi Pranita Ms., Keren Susan Cherian [email protected], Kommi Kalpana Dr

International Journal of Exercise Science

International Journal of Exercise Science 16(2): 1398-1412, 2023. Ice-slurry prepared from plain ice, crushed ice, or sports drink has been used as a cooling strategy before exercise to regulate body temperature and improve exercise performance. However, consensus regarding the benefit is unclear. Therefore, the present review aimed to study the effects of pre-exercise ice-slurry ingestion on thermoregulation and exercise performance of highly trained athletes. The Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist was used. The data extraction was done using the search engine Google Scholar, and digital repositories such as Cochrane, Scopus, Medline, …

Modulation Of Chronic Allergic Contact Dermatitis Through B Cells Reprogramming, Sara Saeed Alhmoudi

Thesis/ Dissertation Defenses

Allergic contact dermatitis (ACD) is a common inflammatory disease caused by contact with an allergen and is a T-cell mediated type IV hypersensitivity reaction. Short chain fatty acids (SCFAs) showed the ability to modulate immune cells in case of inflammation. Pentanoate is one of the abundant SCFA that showed therapeutic ability by inducing IL-10 secretion in lymphocytes. IL-10 producing regulatory B cells have shown to decrease different inflammatory responses; however, overall role of B cells in ACD is not well studied. This project aims to explore the role of B cells during chronic ACD and their modulation with the SCFA …

A Novel Machine-Learning Framework Based On A Hierarchy Of Dispute Models For The Identification Of Fish Species Using Multi-Mode Spectroscopy, Mitchell Sueker, Amirreza Daghighi, Alireza Akhbardeh, Nicholas Mackinnon, Gregory Bearman, Insuck Baek, Chansong Hwang, Jianwei Qin, Amanda M. Tabb, Jiahleen Roungchun, Rosalee S. Hellberg, Fartash Vasefi, Moon Kim, Kouhyar Tavakolian, Hossein Kashini Zadeh

Food Science Faculty Articles and Research

Seafood mislabeling rates of approximately 20% have been reported globally. Traditional methods for fish species identification, such as DNA analysis and polymerase chain reaction (PCR), are expensive and time-consuming, and require skilled technicians and specialized equipment. The combination of spectroscopy and machine learning presents a promising approach to overcome these challenges. In our study, we took a comprehensive approach by considering a total of 43 different fish species and employing three modes of spectroscopy: fluorescence (Fluor), and reflectance in the visible near-infrared (VNIR) and short-wave near-infrared (SWIR). To achieve higher accuracies, we developed a novel machine-learning framework, where groups of …

Common Seastar, Elizabeth T. German Ms., Amy C. Hirons

Hirons Field Techniques

full seastar specimen

length 2.8 cm; width 2.5 cm

Evaluating The Response Of Glycine Soja Accessions To Fungal Pathogen Macrophomina Phaseolina During Seedling Growth, Shirley Jacquet, Layla Rashad, Sonia Viera, Francisco Reta, Juan Reta

Biological Science Student Working Papers

Charcoal rot caused by the fungal pathogen Macrophomina phaseolina (Tassi) Goid is one of various devastating soybean (Glycine max (L.) Merr.) diseases, which can severely reduce crop yield. The investigation into the genetic potential for charcoal rot resistance of wild soybean (Glycine soja) accessions will enrich our understanding of the impact of soybean domestication on disease resistance; moreover, the identified charcoal rot-resistant lines can be used to improve soybean resistance to charcoal rot. The objective of this study was to evaluate the resistance of wild soybean accessions to M. phaseolina at the seedling stage and thereby select the disease-resistant lines. …

The Golden Native Drone Fly (Eristalinus Punctulatus) Is An Effective Hybrid Carrot Pollinator That Lives Within Australian Crop Agroecosystems, Abby E. Davis, Lena A. Schmidt, Karen C B S Santos, Lucie Martin, Samantha Harrington, Marizio Rocchetti, Brad Hocking, Derek Wright, Cameron J. Spurr, David F. Cook, Romina C. Rader

Horticulture Research Articles

1. Native insect flower visitors can be important contributors to crop pollination, yet little is known of their pollination abilities and the resources (habitat) they need to be supported within crop agroecosystems.
2. Here, we compared the abundance and pollination abilities of the golden drone fly (Eristalinus punctulatus) to the European honey bee (Apis mellifera) in hybrid carrot crop fields known to produce variable seed yields in regional New South Wales, Australia. We further observed the egg-laying behaviours of female golden drone flies at a commercial berry orchard to provide insight into the habitat needs of this species. …

Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward …

Predicting Current And Future Alfalfa Hay Prices In Seven Western States, Dillon M. Feuz, Ryan Larsen

All Current Publications

Alfalfa hay prices were analyzed in the seven western states. Utah hay prices were most closely correlated with Oregon and Washington hay prices. Corn grain, feeder cattle, and milk prices all impact alfalfa hay prices, as do the May 1 USDA hay stocks and alfalfa hay exports. Using these price relationships, an alfalfa hay pricing tool was developed to predict hay prices up to 9 months into the future.

The Quest For Immunity: Exploring Human Herpesviruses As Vaccine Vectors, Mohammed S. Kamel, Rachel A. Munds, Mohit S. Verma

Purdue University Libraries Open Access Publishing Fund

Herpesviruses are large DNA viruses that have long been used as powerful gene therapy tools. In recent years, the ability of herpesviruses to stimulate both innate and adaptive immune responses has led to their transition to various applications as vaccine vectors. This vaccinology branch is growing at an unprecedented and accelerated rate. To date, human herpesvirus-based vectors have been used in vaccines to combat a variety of infectious agents, including the Ebola virus, foot and mouth disease virus, and human immunodeficiency viruses. Additionally, these vectors are being tested as potential vaccines for cancer-associated antigens. Thanks to advances in recombinant DNA …

T-Bet And Eomes Are Required For Mature Nk Cell Homeostasis And Function, Pamela Wong

Arts & Sciences Graduate Student Theses and Dissertations

Natural Killer (NK) cells are innate lymphocytes known for their role in recognizing and killing virus-infected or tumor cells without prior sensitization. NK cells express a variety of inhibitory and activating receptors that control their effector functions. Once activated by target cells, cytokines, or activating receptor ligation, NK cells are capable of potent IFN-γ secretion in addition to direct cytotoxicity mediated by perforin and granzymes. Because of their anti-tumor function, NK cells are currently utilized in promising immunotherapy approaches. NK cells develop in the bone marrow and arise from the common lymphoid progenitor, which also gives rise to T, B, …

Trna Methyltransferases Mediate Acinetobacter Baumannii Pathobiology, Jenna Mcguffey

Arts & Sciences Graduate Student Theses and Dissertations

Bacterial pathogens commonly encounter hostile conditions, including disinfectants, antimicrobials, and the host immune system. To be successful, they must adapt through dynamic and complex regulatory mechanisms, such as post-transcriptional modifications on a variety of molecules, including tRNA. In recent years, this tRNA “epitranscriptome” has been increasingly appreciated as an under-researched mechanism for bacterial stress responses. These modifications can impact the translation efficiency of a cohort of stress response factors whose transcripts are enriched for the corresponding codons. However, the variety and roles of tRNA modification enzymes in human pathogens is still largely unknown. The Gram-negative pathogen Acinetobacter baumannii is currently …

Shining A Light Through A Multiomic Lens On Germline Variants: Patterns And Functional Implications Across Cancer Types, Fernanda Martins Rodrigues

Arts & Sciences Graduate Student Theses and Dissertations

The landscape of cancer research has undergone a profound transformation, transcending traditional tissue-centric viewpoints to embrace a more intricate understanding of cellular origins and molecular drivers. It has become evident that genes and variants implicated in specific cancers extend their influence across a spectrum of cancer types. Our prior work, encompassing an >10K cases from The Cancer Genome Atlas (TCGA) and more than >1K pediatric cases, illuminated the intricate web of germline predisposition variants and genes spanning 33 cancer types. These findings not only contributed to the refinement of guidelines for rare germline variant detection but also enriched the clinical …

Uncovering The Genetic Basis Underlying The Pseudomonas Syringae Pv. Tomato Strain Dc3000’S Response To Iaa And Its Role In Regulating Ptodc3000 Biology, Joshua Michael Brian Johnson

Arts & Sciences Graduate Student Theses and Dissertations

Indole-3-acetic acid (IAA) is a molecule ubiquitous to the environment and is known to be produced across all three domains of life. It is most well-known for its role as an important plant hormone (an auxin) and until recently, its roles in other organisms have not been explored. It is well established that bacteria, and pathogens in particular, need to sense and respond to environmental signals. One model organism for studying plant pathogen interactions that is known to both produce and respond to IAA is Pseudomonas syringae pv. tomato strain DC3000 (PtoDC3000). Many plant metabolites have been explored as potential …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern US, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean monthly …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …

Is The Direct Fixation Of Displaced Quadrilateral Plates In Acetabular Fractures Necessary?, Beom-Soo Kim, Ki-Cheor Bae, Chul-Hyun Cho, Kyung-Jae Lee, In Gyu Lee, Byung-Woo Min

Faculty, Staff and Student Publications

Quadrilateral plate fractures represent a heterogeneous group of acetabular fractures. Accurate reduction is required to prevent post-traumatic arthritis. The purpose of this study is to determine the reduction effect of the direct fixation of quadrilateral plates in acetabular fractures, and to evaluate the strength of direct fixation compared to indirect fixation. Between 2005 and 2021, 49 patients underwent surgery for open reduction and internal fixation in acetabular fractures with severely displaced quadrilateral plates. Twenty-nine patients comprised the indirect fixation group, and twenty patients comprised the direct fixation group. In a comparison of primary outcome between two groups, 10 out of …

Venetoclax Abrogates The Prognostic Impact Of Splicing Factor Gene Mutations In Newly Diagnosed Acute Myeloid Leukemia, Jayastu Senapati, Samuel Urrutia, Sanam Loghavi, Nicholas J Short, Ghayas C Issa, Hussein A Abbas, Naveen Pemmaraju, Sherry Pierce, Kelly S Chien, Koji Sasaki, Tapan M Kadia, Danielle E Hammond, Gautam Borthakur, Keyur Patel, Farhad Ravandi, Hagop M Kantarjian, Guillermo Garcia-Manero, Courtney D Dinardo

Faculty, Staff and Student Publications

Mutations in splicing factor (SF) genes SRSF2, U2AF1, SF3B1, and ZRSR2 are now considered adverse risk in the European LeukemiaNet 2022 acute myeloid leukemia (AML) risk stratification. The prognostic impact of SF mutations in AML has been predominantly derived from younger patients treated with intensive (INT) therapy. We evaluated 994 patients with newly diagnosed AML, including 266 (27%) with a SFmut. Median age was 67 years overall, with patients with SFmut being older at 72 years. SRSF2 (n = 140, 53%) was the most common SFmut. In patients treated with INT, median relapse-free survival (RFS) (9.6 vs 21.4 months, P …

Cd46-Adc Reduces The Engraftment Of Multiple Myeloma Patient-Derived Xenografts, Michael J Vanwyngarden, Zachary J Walker, Yang Su, Olivia Perez De Acha, Brett M Stevens, Tomer M Mark, Bin Liu, Daniel W Sherbenou

Faculty, Staff and Student Publications

An antibody-drug conjugate (ADC) targeting CD46 conjugated to monomethyl auristatin has a potent anti-myeloma effect in cell lines in vitro and in vivo, and patient samples treated ex vivo. Here, we tested if CD46-ADC may have the potential to target MM-initiating cells (MM-ICs). CD46 expression was measured on primary MM cells with a stem-like phenotype. A patient-derived xenograft (PDX) model was implemented utilizing implanted fetal bone fragments to provide a humanized microenvironment. Engraftment was monitored via serum human light chain ELISA, and at sacrifice via bone marrow and bone fragment flow cytometry. We then tested MM regeneration in PDX by …

Characterization Of The Probiotic Properties Of Yeast Strains Isolated From Different Food Sources And Unraveling The Potential Nutritional Benefits Of Fermented Date Palm Pomace With Selected Probiotic Candidates: Untargeted Metabolomics And Carbohydrate Metabolites Of In Vitro Digested Fraction, Nadia Al Kalbani

Thesis/ Dissertation Defenses

Utilizing fermentation with probiotics offers a promising opportunity to leverage agricultural by-products, such as date palm pomace (DPP), in order to drive forward sustainable food production endeavors. This dissertation aimed to (1) isolate yeast from fermented food products as well as to characterize their probiotic properties; (2) investigate the physiochemical properties of fermented DPP by selected yeast isolates; (3) evaluate the health benefits of non-fermented and fermented DPP samples both pre and post-digestion (bioaccessible portions); and (4) analyze the untargeted metabolites and carbohydrates metabolites in the bioaccessible portions. The yeast isolates showed remarkable survivability in acidic environment while bile salt …

Opening Speech, Ray Smith

IGC Proceedings (1977-2023)

https://youtu.be/CbRcQzf5z0o?si=ESOHxXF-jFsYMw2A

Biodiversity—The Birds And The Bees And Healthy Grasslands, D. Borrenpohl, Pat D. Keyser

IGC Proceedings (1977-2023)

Losses in biodiversity abound in modern agroecosystems, and biodiversity loss hampers ecosystem function and primary productivity comparable to abiotic stress. One of the most endangered ecosystems in the world is North American tallgrass prairie, and native birds and pollinators that historically depended on tallgrass prairie are in precipitous decline. Fortunately, native warm-season grasses that dominated tallgrass prairie present a valuable opportunity for summer forage to beef producers in the Eastern United States—a time when coolseason grasses endure a lack in productivity called the “summer slump.” Tallgrass prairie was sustained by periodic disturbance from grazing and fire, and combining fire and …

The Soil Health Properties Of Native Grasslands, C. Bitler, Pat Keyser

IGC Proceedings (1977-2023)

Due to an increased interest in soil health and the role soils can have in carbon sequestration, native grasslands are getting heightened attention. Native grasslands are known for their deep top-soils that develop over time through the accumulation of soil organic matter. The deep and fibrous root systems that make up two-thirds of the biomass of native grasslands are the primary contributors to the soil organic carbon (SOC) content of grassland soils. Increased SOC content has a cascading effect on soil health metrics through increasing water infiltration and water holding capacity, supporting diverse and abundant soil microbial life, and improving …

Using Functional Ecology To Predict Restoration Success In Managed Grasslands, J. Bergman

IGC Proceedings (1977-2023)

Plants have developed remarkable functional variation over evolutionary time to cope with different environmental conditions. As a result, functional variation – measurable as functional traits above and below ground – can be found within but also among grassland communities today. The functional space covered by a community is thereby restricted by environmental factors while each single species within the community inhabits a specific niche with a certain breadth along different environmental gradients. Data from field sampling in European grasslands have shown, that species intraspecific functional variation can cover considerable amounts of possible functional spaces, hence holding the potential to establish …

Toxic Plants In Grasslands And Their Impact On Livestock, A. C. Barr

IGC Proceedings (1977-2023)

Grasslands are critical infrastructure for most cow/calf operations. Quality and abundance of pasture grasses directly influence livestock rate of gain, thereby affecting livestock margins and production costs, which determine the profitability of stocker operations. Successful cattlemen control weeds to preserve healthy pasture but also to prevent plant poisonings that may reduce individual animal performance or cause fatalities. Integrated Toxic Plant Management (ITPM) programs teach ranchers to identify the potentially toxic plants, recognize symptoms of plant poisonings, and use appropriate strategies to control those plants. Toxic plants can produce a range of effects in cattle and other grazing species. Nightshades ( …

Response Of Reproductive Phenology To Soil Water Content Of Stipa Breviflora Under Warming And Nitrogen Application, L. Bai, H. Y. Ren, G. D. Han

IGC Proceedings (1977-2023)

Plant phenology is considered as the most direct and sensitive characteristic indicators of plant response to regional climate change. Based on the observation data of phenology and soil water content of Stipa breviflora in an experimental platform of warming and nitrogen application in Inner Mongolia desert steppe from 2009 to 2019. The results showed that warming and nitrogen application significantly advanced the reproductive phenological period (P < 0.001), and the interaction of warming and nitrogen application promoted the advance of reproductive phenological period (P < 0.05).

Conference Agenda, Igc

IGC Proceedings (1977-2023)

No abstract provided.

Covid‑19‑Related Risk, Resilience, And Mental Health Among Mexican American Mothers Across The First Year Of The Pandemic, Amy L. Non, Elizabeth S. Clausing, Sandraluz Lara‑Cinisomo, Kimberly L. D’Anna Hernandez

Center for Brain, Biology, and Behavior: Faculty Publications

Background Latina mothers have been especially affected by the pandemic and historically exhibit high rates of depression and anxiety. However, few longitudinal studies have assessed the effect of the pandemic on this vulnerable population. We hypothesized that COVID-19-related stressors would associate with psychological distress among Latina mothers across the first year of the pandemic.

Methods We investigated COVID-19-related impact, stigma, and fears across two critical time points and changes in these measures in relation to changes in maternal anxiety and depression among mothers of Mexican descent living in Southern California (n=152). Surveys were administered within 5–16 weeks of …

Integrative Transcriptomics And Proteomics Profiling Of Arabidopsis Thaliana Elucidates Novel Mechanisms Underlying Spaceflight Adaptation, Gbolaga O. Olanrewaju, Natasha J. Haveman, Michael J. Naldrett, Anna-Lisa Paul, Robert J. Ferl, Sarah E. Wyatt

Nebraska Center for Biotechnology: Faculty and Staff Publications

Spaceflight presents a unique environment with complex stressors, including microgravity and radiation, that can influence plant physiology at molecular levels. Combining transcriptomics and proteomics approaches, this research gives insights into the coordination of transcriptome and proteome in Arabidopsis’ molecular and physiological responses to Spaceflight environmental stress. Arabidopsis seedlings were germinated and grown in microgravity (μg) aboard the International Space Station (ISS) in NASA Biological Research in Canisters – Light Emitting Diode (BRIC LED) hardware, with the ground control established on Earth. At 10 days old, seedlings were frozen in RNA-later and returned to Earth. RNA-seq transcriptomics and TMTlabeled LC-MS/MS proteomic …