Life Sciences Commons^™

Eragrostis Curvula Effects On Above And Below-Ground Plant Species Richness And Diversity, Jed Brown, A. Merchant, L. Ingram

IGC Proceedings (1977-2023)

Monitoring and managing the soil seed bank is fundamental to land management as it constitutes the future generations of invasive plant communities. Invasive plants have traits that result in high recruitment through increased seed generation, short seed dormancy and phenotypic plasticity. Furthermore, invasive plants with growth forms that inhibit the growth and recruitment of other species can lead to monocultures and associated reduction in above-ground biodiversity, potentially negatively impacting the soil seed bank diversity and ecosystem functions and services. Eragrostis curvula is one such species that has many of these invasive traits, including high propagule generation, and can exclude plant …

The Importance Of Forage Legume Epigenetics In The Anthropocene, J. Brady, J. P. Muir, A. Falk

IGC Proceedings (1977-2023)

As climates change and economic as well as ecological pressures to produce more ruminant product on less land in the Anthropocene increase, the importance of using cutting-edge methods for forage legume improvement grows. The immediate return rate and turnaround on investment is likely greater for multi-species incorporation into grasslands but focusing on intraspecific variability and diversity could also contribute. The role of forage legumes in cultivated pasture and rangeland biodiversity as well as stability depends on genetics as well as management. Their epigenetics, however, may become increasingly important as climatic extremes and ecological pressures grow due to human-induced factors. Unfortunately, …

Identification And Characteristics Of Phosphate-Solubilizing Endophytic Bacteria From Root Nodules Of Lespedeza Daurica, K. Y. Yang, C. Huang, P. Gao, Y. P. Liang, Y. P. Han, X. Zhao

IGC Proceedings (1977-2023)

Endophytic phosphate-solubilizing bacteria from root nodules could promote stress resistance, nodulation and nitrogen fixation. In this study, nodule endophytic bacteria of Lespedeza daurica were isolated by plate streaking, the bacteria with phosphate-solubilizing abilities were selected and the phosphate-solubilizing abilities were determined. The results showed that 88 endophytic bacterial strains were isolated and among which 11 strains possessed phosphate-solubilizing abilities. The organic phosphorus solubility of TG41, TG43, TG47 and TG68 were 0.73~0.86 μg·mL^-1 ; the inorganic phosphorus solubility of TG47 and TG68 were 72.97 and 1.29 μg·mL^-1 , respectively. The four strains were identified as effective phosphate-solubilizing bacteria. TG41 …

Rangeland Health Status And Condition Two Different Yet Complementary Concepts: National Reserve Pampa Galeras Barbara D´Achille Case, R. Zárate, E. R. Flores

IGC Proceedings (1977-2023)

Pampa Galeras Barbara D´Achille National Reserve has an extension of 21,030 hectares constituted mainly by puna rangeland and has played a key role in the recovery of the vicuña (Vicugna vicugna) population from extinction. This reserve is divided into two zones, known as the Rigid zone with a total area of 6,500 ha, and the Buffer zone of 14,530 ha. The present study aimed to implement an Ecological Response Unit system (ERU) to create an evaluation and monitoring system of rangeland health and condition, that could serve as a model for the rest of the Protected National Areas …

Assessing The Usefulness Of Senna Obtusifolia, An Invasive Plant Species In West Africa Rangelands, A. Zaré, O. Ouédraogo, A. Linstädter

IGC Proceedings (1977-2023)

Senna obtusifolia is a less appreciated plant species. Its invasion has led to the disappearance of several herbaceous species of interest in West Africa rangelands. As adaptation strategy, people use it to substitute for those that have disappeared. This study aimed to assess the importance of this species for animal and local ethnic groups according to two contrast climate zones in Burkina Faso. Ethnobotanic survey mixed with direct observations on the field were conducted. Three hundred (300) people from height (8) ethnic groups were interviewed. The survey technique was semi-structured interviews using a previously tested semi-structured questionnaire. At the early …

Responses Of Plant Functional Diversity To Long-Term Warming And Nitrogen Addition In A Desert Steppe, Y. Zhu, L. Tian, H. Y. Ren, G. D. Han

IGC Proceedings (1977-2023)

Plant functional traits are closely related to ecosystem functions, and can indicate changes in ecosystem functions. Plant functional diversity is an important component of biodiversity, which can more accurately predict the changes of ecosystem functions or processes than species diversity, and is the main determinant of ecosystem functions or processes. In order to study the impact of global climate change factors (i.e., warming and nitrogen deposition) on the plant functional diversity in a desert steppe, we conducted a long-term warming and nitrogen addition experiment in northern China for 15 years (2006 - 2020). In 2020, we measured the plant height, …

Evaluating Herbicides For The Control Of Multiflora Rose And Autumn Olive In Eastern Ohio, C. Zoller, D. Kreager, T. G. Wiseman, C. Martin, A. Essman, C. Gelley, C. Little, M. Loux

IGC Proceedings (1977-2023)

Invasive plants cause billions of dollars annually in damage, displace desirable plants, and reduce available land area for livestock grazing. Multiflora rose and autumn olive are two such species prevalent across the Eastern United States. Originally introduced for livestock containment and erosion control, these plants have become widespread, resulting in reduced animal grazing. The Eastern Agricultural Research Station (EARS) is located in Southeast Ohio and is owned by The Ohio State University. Much of the more than 2,000 acres has a history of surface mining and was reclaimed with the planting of multiflora rose and autumn olive. These plants have …

The Impact Of Appearance-Related Communication In Families On Female Adolescent Body Image Development, Cambria Siddoway

Family Perspectives

No abstract provided.

Dyadic Coping In The Transition To Parenthood, Sierra Gray

Family Perspectives

The Transition to Parenthood (TTP) is a common transition point for couples that is often associated with declines in relationship satisfaction. There are many changes that take place during this transition that can lead to a variety of stresses such as psychological distress, sexual distress, as well as increases in conflict. Parents likely experience these stressors together, and therefore should implement dyadic coping processes. With partner support and responsiveness, parents can counter damaging psychological outcomes. As partners incorporate empathy and communicate effectively throughout the TTP, they can prevent and improve sexual outcomes. Since partner conflict is very common during this …

More Than Fun And Games: Why Play Matters, Leah Merrill

Family Perspectives

No abstract provided.

How To Make Prison Visits A Success, Ellie Anderson

Family Perspectives

No abstract provided.

Association Of The Abcb1 Gene Polymorphism And Infertility In Azoospermia Males In Iraqi Kurdish Population, Muhsin Jamil Abdulwahid

Polytechnic Journal

The P-glycoprotein (P-gp), a product of the ABCB1 gene (ATP binding cassette subfamily B member 1) or MDR: multiple drug resistance 1 gene is expressed in various parts of the human body, like testis. The ABCB1 gene has several single nucleotide polymorphisms in its exons and introns that have been discovered. This study used DNA sequencing and polymerase chain reaction (PCR) to discover and characterize nucleotide variations in exons 27 of the ABCB1 gene in seven infertile and three normally fertile individuals. In this study, a set of primers were designed using the NCBI primer designing tool to amplify 394bp …

A Novel Subcluster Of Closely Related Bacillus Phages With Distinct Tail Fiber/Lysin Gene Combinations, Rachel E. Loney, Veronique A. Delesalle, Brianne E. Chaudhry, Megan Czerpak, Alexandra A. Guffey, Leo Goubet-Mccall, Michael Mccarty, Madison S. Strine, Natalie T. Tanke, Albert C. Vill, Gregory P. Krukonis

Biology Faculty Publications

Bacteriophages (phages) are the most numerous entities on Earth, but we have only scratched the surface of describing phage diversity. We isolated seven Bacillus subtilis phages from desert soil in the southwest United States and then sequenced and characterized their genomes. Comparative analyses revealed high nucleotide and amino acid similarity between these seven phages, which constitute a novel subcluster. Interestingly, the tail fiber and lysin genes of these phages seem to come from different origins and carry out slightly different functions. These genes were likely acquired by this subcluster of phages via horizontal gene transfer. In conjunction with host range …

Cortisol And Epinephrine Alter The Adipose Functions And The Mobilization Of Pcbs In Adipose Tissue Slices From Elephant Seal, Laura Pirard, Jane I. Khudyakov, Daniel E. Crocker, Liesbeth Van Hassel, Georges Scholl, Gauthier Eppe, Melissa M. Page, Jean-François Rees, Donald R. Smith, Cathy Debier

Pacific Faculty Work

Marine mammals are exposed to increasing intensities of anthropogenic stressors such as acoustic disturbance and contaminants. Correlative studies have suggested concerning shifts in behavioral and physiological status of stress-exposed individuals, which could alter the health and survival of marine mammal populations. However, functional studies of the effects of multiple stressors on marine mammals are lacking. To fill in this gap, we recently developed an ex vivo approach of precision-cut adipose tissue slices (PCATS) to study the impact of stressors on the function of an essential endocrine organ: the adipose tissue. In the present study, we investigated the impact of hormones …

Channel Catfish (Ictalurus Punctatus), Neurocranium, Corinne Renshaw, Brianna Buckley, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 4.9 cm total length 20.6 cm Ictalurus punctatus (Channel Catfish), collected from Dr. Kerstetter's dissection specimens.

Oregon State Rank Assessment For Montane Peaclam (Pisidium Ultramontanum), Misty Nelson

Institute for Natural Resources Publications

Oregon State Rank Assessment for montane peaclam (Pisidium ultramontanum) using NatureServe methodology, 2023.

A Snapshot Into The Lives Of Elephants: Camera Traps And Conservation In Etosha National Park, Namibia, Jodie L. Berezin, Amanda J. Odom, Virginia Hayssen, Caitlin E. O'Connell-Rodwell

Biological Sciences: Faculty Publications

Knowledge of elephant movement and grouping patterns in the wild is critical for their management and conservation. Much of these data come from GPS collar data and aerial surveys, which have provided invaluable information, but data from these methods are often limited to small groups or entire populations. Effective elephant management requires both generalized and localized methodologies. Here, we propose the expanded use of camera traps in research relating to elephant localized movements and grouping patterns as an additional tool for elephant conservation management. In this study, we use a battery-powered camera trap to provide daily high-resolution data of African …

The Transcription Factor Irf4 Determines The Anti-Tumor Immunity Of Cd8+ T Cells, Xiang Xiao, Jinfei Fu, Dawei Zou, Anze Yu, Tao Jiang, Xian C Li, Zhongming Zhao, Wenhao Chen

Faculty, Staff and Student Publications

Understanding the factors that regulate T cell infiltration and functional states in solid tumors is crucial for advancing cancer immunotherapies. Here, we discovered that the expression of interferon regulatory factor 4 (IRF4) was a critical T cell intrinsic requirement for effective anti-tumor immunity. Mice with T-cell-specific ablation of IRF4 showed significantly reduced T cell tumor infiltration and function, resulting in accelerated growth of subcutaneous syngeneic tumors and allowing the growth of allogeneic tumors. Additionally, engineered overexpression of IRF4 in anti-tumor CD8+ T cells that were adoptively transferred significantly promoted their tumor infiltration and transition from a naive/memory-like cell state into …

An Open Natural Language Processing (Nlp) Framework For Ehr-Based Clinical Research: A Case Demonstration Using The National Covid Cohort Collaborative (N3c), Sunyang Fu, Robert Miller, Andrew Williams, Daniel Harris, Ramakanth Kavuluru, Mei Liu, Noor Abu-El-Rub, Dalton Schutte, Rui Zhang, Masoud Rouhizadeh, John D Osborne, Yongqun He, Umit Topaloglu, Stephanie S Hong, Joel H Saltz, Thomas Schaffter, Emily Pfaff, Christopher G Chute, Tim Duong, Melissa A Haendel, Rafael Fuentes, Peter Szolovits, Hua Xu, Hongfang Liu

Faculty, Staff and Student Publications

Despite recent methodology advancements in clinical natural language processing (NLP), the adoption of clinical NLP models within the translational research community remains hindered by process heterogeneity and human factor variations. Concurrently, these factors also dramatically increase the difficulty in developing NLP models in multi-site settings, which is necessary for algorithm robustness and generalizability. Here, we reported on our experience developing an NLP solution for Coronavirus Disease 2019 (COVID-19) signs and symptom extraction in an open NLP framework from a subset of sites participating in the National COVID Cohort (N3C). We then empirically highlight the benefits of multi-site data for both …

Coordination Of Rooting, Xylem, And Stomatal Strategies Explains The Response Of Conifer Forest Stands To Multi-Year Drought In The Southern Sierra Nevada Of California, Junyan Ding, Polly Buotte, Roger Bales, Bradley O. Christoffersen, Rosie A. Fisher, Michael Goulden, Ryan Knox, Lara Kueppers, Jacquelyn Shuman, Chonggang Xu, Charles D. Koven

School of Integrative Biological & Chemical Sciences Faculty Publications

Extreme droughts are a major determinant of ecosystem disturbance that impacts plant communities and feeds back into climate change through changes in plant functioning. However, the complex relationships between aboveground and belowground plant hydraulic traits and their role in governing plant responses to drought are not fully understood. In this study, we use a model, the Functionally Assembled Terrestrial Ecosystem Simulator in a configuration that includes plant hydraulics (FATES-Hydro), to investigate ecosystem responses to the 2012–2015 California drought in comparison with observations at a site in the southern Sierra Nevada that experienced widespread tree mortality during this drought.

We conduct …

An Intersection Of Science & Art: Vitrification Approaches And Open-Fabricated Tools For The Biomedical Model Sea Hare, Aplysia Californica, Allyssa M. Oune

LSU Master's Theses

The California sea hare (Aplysia californica) is an important biomedical model for molecular neurobiology, electrophysiology, learning, and memory due to their well-mapped and large neurons and well-characterized learning capabilities. The National Resource for Aplysia (NRA, University of Miami) maintains large stocks of live animals and relies on regular shipments of wild-caught individuals to maintain genetic diversity. This is labor and cost-intensive, and environmental changes could alter the availability of wild animals increasing the need to preserve this genetic resource. One solution is vitrification, ultra-fast cooling which produces an amorphous glass that minimizes damage to cells. Aplysia californica presents …

Reduced Grey Matter Volume In Adolescents With Conduct Disorder: A Region-Of-Interest Analysis Using Multivariate Generalized Linear Modeling, Ru Zhang, R James R Blair, Karina S Blair, Matthew Dobbertin, Jaimie Elowsky, Ahria J Dominguez, Sahil Bajaj

Faculty, Staff and Student Publications

Background: Conduct disorder (CD) involves a group of behavioral and emotional problems that usually begins during childhood or adolescence. Structural brain alterations have been observed in CD, including the amygdala, insula, ventrolateral and medial prefrontal cortex, anterior cingulate cortex, and fusiform gyrus. The current study developed a multivariate generalized linear model (GLM) to differentiate adolescents with CD from typically developing (TD) adolescents in terms of grey matter volume (GMV).

Methods: The whole-brain structural MRI data were collected from 96 adolescents with CD (mean age = [Formula: see text] years; mean IQ = [Formula: see text]; 63 males) and 90 TD …

Rapid, High Throughput, Automated Detection Of Sars-Cov-2 Neutralizing Antibodies Against Wuhan-Wt, Delta And Omicron Ba1, Ba2 Spike Trimers, Narayanaiah Cheedarla, Hans P Verkerke, Sindhu Potlapalli, Kaleb Benjamin Mclendon, Anamika Patel, William Henry O'Sick, Tyler Jon Baugh, Gregory L Damhorst, Huixia Wu, Daniel Graciaa, Fuad Hudaib, David N Alter, Janetta Bryksin, Eric A Ortlund, Jeanette Guarner, Sara Auld, Sarita Shah, Wilbur Lam, Dawn Mattoon, Joseph M Johnson, David H Wilson, Madhav V Dhodapkar, Sean R Stowell, Andrew S Neish, John D Roback

Faculty, Staff and Student Publications

Traditional cellular and live-virus methods for detection of SARS-CoV-2 neutralizing antibodies (nAbs) are labor- and time-intensive, and thus not suited for routine use in the clinical lab to predict vaccine efficacy and natural immune protection. Here, we report the development and validation of a rapid, high throughput method for measuring SARS-CoV-2 nAbs against native-like trimeric spike proteins. This assay uses a blockade of human angiotensin converting enzyme 2 (hACE-2) binding (BoAb) approach in an automated digital immunoassay on the Quanterix HD-X platform. BoAb assays using Wuhan-WT (vaccine strain), delta (B.1.167.2), omicron BA1 and BA2 variant viral strains showed strong correlation …

Revisiting The Use Of Normal Saline For Peritoneal Washing In Ovarian Cancer, Hironari Akasaka, Honami Naora

Faculty, Staff and Student Publications

The omentum is the predominant site of ovarian cancer metastasis, but it is difficult to remove the omentum in its entirety. There is a critical need for effective approaches that minimize the risk of colonization of preserved omental tissues by occult cancer cells. Normal saline (0.9% sodium chloride) is commonly used to wash the peritoneal cavity during ovarian cancer surgery. The omentum has a prodigious ability to absorb fluid in the peritoneal cavity, but the impact of normal saline on the omentum is poorly understood. In this review article, we discuss why normal saline is not a biocompatible solution, drawing …

Pmca Screening Of Retropharyngeal Lymph Nodes In White-Tailed Deer And Comparisons With Elisa And Ihc, Rebeca Benavente, J Hunter Reed, Mitch Lockwood, Rodrigo Morales

Faculty, Staff and Student Publications

Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified …

Dynamics Of Cwd Prion Detection In Feces And Blood From Naturally Infected White-Tailed Deer, Francisca Bravo-Risi, Paulina Soto, Rebeca Benavente, Tracy A Nichols, Rodrigo Morales

Faculty, Staff and Student Publications

Chronic wasting disease (CWD) is a prion disease affecting cervids. Confirmatory testing of CWD is currently performed postmortem in obex and lymphoid tissues. Extensive evidence demonstrates the presence of infectious prions in feces of CWD-infected deer using in vitro prion-amplification techniques and bioassays. In experimental conditions, this has been achieved as soon as 6-month post-inoculation, suggesting this sample type is a candidate for antemortem diagnosis. In the present study, we optimized the detection of CWD-prions in fecal samples from naturally infected, pre-clinical white-tailed deer by comparing protocols aiming to concentrate CWD-prions with direct spiking of the sample into the PMCA …

The View From Ventress - 2022-23 Academic Year, University Of Mississippi, College Of Liberal Arts

Liberal Arts Newsletters

News from the College of Liberal Arts

Enzyme Kinetics Of Deoxyuridine Triphosphatase From Western Corn Rootworm, Carlos Riera‑Ruiz, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

Objective The Western corn rootworm (WCR), Diabrotica virgifera virgifera, is a highly adaptable insect pest that has evolved resistance to a variety of control strategies, including insecticides. Therefore, it is interesting to examine how housekeeping proteins in WCR have been changed under WCR-controlling strategies. In this study, we focused on one of such proteins in WCR, a ubiquitous enzyme 5’-triphosphate nucleotidohydrolase (dUTPase). In the thymidine synthetic pathway, dUTPase hydrolyzes deoxyuridine triphosphate (dUTP) and supplies the substrate, deoxyuridine monophosphate, for the thymidylate synthase (TS). It decreases the cellular content of uracil, reducing uracil misincorporation into DNA. Suppressing the dUTPase activity, …

Effects Of Doxorubicin On Extracellular Matrix Regulation In Primary Cardiac Fibroblasts From Mice, Cameron Skaggs, Steve Nick, Conner Patricelli, Laura Bond, Kali Woods, Luke Woodbury, Julia Thom Oxford, Xinzhu Pu

Biomedical Research Institute Publications and Presentations

Objective Doxorubicin (DOX) is a highly effective chemotherapeutic used to treat many adult and pediatric cancers. However, its use is limited due to a dose-dependent cardiotoxicity, which can lead to lethal cardiomyopathy. In contrast to the extensive research efforts on toxic effects of DOX in cardiomyocytes, its effects and mechanisms on cardiac extracellular matrix (ECM) homeostasis and remodeling are poorly understood. In this study, we examined the potential effects of DOX on cardiac ECM to further our mechanistic understanding of DOX-induced cardiotoxicity.

Results DOX-induced significant down-regulation of several ECM related genes in primary cardiac fibroblasts, including Adamts1, Adamts5, Col4a1, Col4a2, …

Engaging Students In A Genetics Course-Based Undergraduate Research Experience Utilizing Caenorhabditis Elegans In Hybrid Learning To Explore Human Disease Gene Variants, Natalie Forte, Virginia Veasey, Bethany Christie, Amira Carter, Marli Hanks, Alan Holderfield, Taylor Houston, Anil Challa, Ashley Turner

Research, Publications & Creative Work

Genetic analysis in model systems using bioinformatic approaches provides a rich context for a concrete and conceptual understanding of gene structure and function. With the intent to engage students in research and explore disease biology utilizing the nematode Caenorhabditis elegans model, we developed a semester-long course-based undergraduate research experience (CURE) in a hybrid (online/in-person) learning environment—the gene-editing and evolutionary nematode exploration CURE (GENE-CURE). Using a combination of bioinformatic and molecular genetic tools, students performed structure-function analysis of disease-associated variants of uncertain significance (VUS) in human orthologs. With the aid of a series of workshop-style research sessions, students worked in teams …