Life Sciences Commons^™

Annotation Of Bacteriophages Redraider89, Superherocarly, Cherrycola, And Kouch, Brianne Brandt, Mya Cole, Matthew Dawkins, Julia De Vos, Isaac De Young, Hannah Gordon, Landon Greve, Brody Hoverstein, Mia Kellenberger, Genesis Maldonado, Charlotte Pritchard, Noah Robinson, Leonardo Romo, Mackenzie Schram, Grace Timm, Arianna Van Donkelaar, Kamea Van Kalsbeek, Byron Noordewier, Sara S. Tolsma

Celebration of Research

Phages are important research tools and can be used as an alternative to antibiotics. They are abundant and diverse. Genome annotation is used to better understand phage diversity as well as individual phages for use in the lab and clinic. We annotated the genomes of four novel phages: RedRaider89, SuperHeroCarly, Kouch, and CherryCola. All four phages were discovered by Northwestern College students. CherryCola is a Cluster DC1 phage that infects Gordonia terrae. It has 92 predicted protein-coding genes. RedRaider89 (Cluster B1) and SuperHeroCarly (Cluster D1) infect Mycobacterium smegmatis mc²155. RedRaider89 has 99 predicted protein-coding genes and SuperHeroCarly has 87 …

Understanding The Role Of Lipid Metabolism On Pancreatic Cancer Metastasis, Luke Fischer, Kylie Foss, Jacob Lewis, Joshua Lewis, Emma Steinhardt, Megan Vangarven, Cody Rozeveld

Celebration of Research

Pancreatic cancer is one of the deadliest types of cancer due to late-stage detection and early metastasis. Other than smoking, one of the few known risk factors for pancreatic cancer is obesity (1). On the cellular level, excess fat can promote cell migration and invasion (2). However, the exact fat utilization pathway remains unknown. We propose that lysosomes may be exploited in pancreatic cancer to break down fat and provide energy for metastasis and invasion.

Quantifying And Validating The Decrease Of Ciliogenesis Genes In Mouse Models With Pcd, Mia X. Kellenberger, Casey Mckenzie, Lance Lee

Celebration of Research

Primary ciliary dyskinesia (PCD) is a rare genetic disease affecting one in 16,000 children and results from structural or functional defects of motile cilia1. Lack of functioning cilia may result in reduced respiratory function, subfertility or infertility, and hydrocephalus due to a reduced ability to clear fluids and particles. A cilium consists of nine microtubules centered around a central pair complex1. Mutations in genes Spef2 (bgh), Cfap221 (nm1054), or Cfap54(Cfap54gt/gt), which encode proteins located in the central pair complex, result in PCD2,3,4. We previously utilized single-cell RNA sequencing to identify differentially expressed genes (DEGs) in early and late deuterosomal cells, …

Bacterial 2-Hybrid Assay: Interactions Of Phage Island3'S Gene 36 And Mycobacterium Smegmatis Gene Products, Elsie L. Larson, Sara Tolsma

Celebration of Research

Island3 is a Cluster I1 bacteriophage that infects Mycobacterium smegmatis. Northwestern College students are performing systematic genetic screens to identify Island3 genes that are cytotoxic or protect host cells from infection by other phages (defense). We identified Island3’s gp36 as a cytotoxic gene. Now we are using a bacterial two-hybrid assay to identify a host protein(s) interaction partner. We hypothesize that host proteins that bind gp36 will reveal the mechanism by which gp36 confers cytotoxicity.

Investigation Of Northwest Iowa Pollinator Communities Using Two Survey Methods, Ellary Carson, Mya Cole, Isaac De Young, Sharon Huffman, Naomi Johnson, Genesis Maldonado, Maelee Rodenburg, Travis Sweeney, Tark Griebel, Laurie Furlong, Todd Tracy

Celebration of Research

The ecological services of insect pollinators are well established and the documented decline in pollinator biodiversity is of great concern. Baseline data and continued monitoring are essential as we ongoing trends in pollinator communities and evaluate efforts supporting their recovery. Using two survey methods, our study provides pollinator community data at two conservation sites in Northwest Iowa. In September of 2025, we used three colors of pan traps (blue, yellow, and white) to investigate pollinator communities at a small, reconstructed prairie (Buena Vista County Park) and a nearby partially restored native prairie (The Nature Conservancy Glacial Hills Preserve). We also …

Geographic Trends In North American Tornado Risk: A 75 Year Analysis, Thomas Tracy, Todd Tracy

Celebration of Research

In recent years, many meteorologists have claimed that ‘tornado alley’ has shifted over the last decades toward the east. We wanted to see if this claim holds true. We searched online for databases containing information on all US and all Canada tornadoes. By visualizing the data, we were able to find some trends in rating and the number of tornadoes over time. Using a program we wrote in R, we calculated the geographical average for tornadoes for each year. We also weighted this average by the rated magnitude of each tornado and by the number of deaths from each tornado. …

Optimizing Seedling Emergence In Sagebrush-Steppe Restoration: The Role Of Sowing Depth, Seed Coatings, And Microsite Amendments, John Thomas Stromberg

Theses and Dissertations

Seedling survival of fall-sown seeds over the winter and subsequent emergence in spring is a major demographic bottleneck limiting recruitment in dryland restoration. Deeper sowing can place seeds in a more favorable soil environment with more stable moisture and moderated temperatures, but emergence from these depths is often constrained by the greater distance seedlings must grow to reach the surface and by mechanical resistance in the upper soil layer. In the first chapter of this thesis, we evaluated the use of a slow-release gibberellic acid (GA₃) polymer seed coating to aid in seedling emergence from deeper sowing depths. We compared …

Recent Wetland Elevation Dynamics In Coastal Louisiana, Usa, Elizabeth Harris

LSU Master's Theses

Coastal marshes globally are increasingly vulnerable to accelerating relative sea-level rise (RSLR), which threatens their capacity to maintain elevation through feedbacks among sediment supply, vegetation productivity, hydrology, and soil processes. Although many marshes can persist under moderate rates of sea-level rise through vertical accretion and belowground biomass production, this resilience is strongly constrained by sediment availability and subsurface processes such as autocompaction and organic matter decomposition. High accretion is often assumed to confer marsh resilience; however, subsurface processes, particularly autocompaction driven by surface loading, can substantially offset elevation gains. Coastal Louisiana experiences among the highest rates of RSLR worldwide due …

Toward A Local Governance Model For Green Building Regulation: Lessons From A Philippine City, Mikaela Amihan S. Arrosa, Aileen V. Lapitan

Journal of Public Affairs and Development

The study analyzed local governance in implementing green building regulations in a city in Metro Manila, Philippines, through a case study. Compliance rates were calculated by comparing projects with Green Building Compliance Certification to those with Green Building Pre-Compliance Certification issued by the city. The Delphi method explored environmental sustainability implications of compliance, while key informant interviews and policy landscape analysis identified the factors affecting Green Building Code management. Fewer than half of the pre-compliance and construction permit holders also received occupancy certificates. Three rounds of the Delphi method were conducted, resulting in consensus on recommendations to enhance governance mechanisms …

Subjective Feelings And Electrophysiological Responses To Love-Stimuli In Parasocial And Real-Life Romantic Love, Magda Martinez-Mata

Theses

People can have a crush on, fall in love with, or develop a romantic interest in media figures despite having no reciprocal interaction, which is known as parasocial love. The objective of this study was to evaluate subjective feelings for and event-related potential (ERP) responses reflecting attention to the parasocial beloved and to compare them to real-life love. Twenty-six participants who have a parasocial beloved were recruited. Sixteen of those had a real-life beloved as well. The participants answered questions about their love feelings and completed the Infatuation and Attachment Scales and the Parasocial Love Scale for both their parasocial …

Molecular Adaptation Of Sweet Potato To Martian Soil Simulant: Insights From Mrna And Long Non-Coding Rna Analyses, Subramanyam Reddy Chinreddy, Karthik Chinnannan, Purushothaman Natarajan, Vaibhavi Patel, Gwonjin Lee, Manohar Chakrabarti, Padma Nimmakayala, Umesh K. Reddy

School of Integrative Biological & Chemical Sciences Faculty Publications

Introduction: Understanding how crops adapt to extraterrestrial environments is essential for sustainable space agriculture. Sweet potato (Ipomoea batatas), a nutritionally rich and stress-resilient crop, is a promising candidate for cultivation under Martian-like conditions, characterized by high salinity, heavy metal contamination, and poor water retention.

Objective: This study aimed to elucidate the molecular mechanisms underlying sweet potato adaptation to Martian soil analog conditions using Mars Global Simulant-1 (MGS-1).

Methods: Leaf, shoot, and storage root tissues of sweet potato grown in MGS-1 were subjected to RNA sequencing. Differentially expressed mRNAs and long non-coding RNAs (lncRNAs) were identified, and functional enrichment analyses were …

Effects Of Gnrh And Hcg Administration On Conception Rate And The Subsequent Estrous Cycle On Day 12 Following Artificial Insemination In Holstein Cows, Mustafa Hi̇lmi̇ Yaranoğlu, Hüseyi̇n Erdem

Turkish Journal of Veterinary & Animal Sciences

The present study investigates the effects of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin administration on the conception rate of cows 12 days after artificial insemination (AI). Included in the study were 150 high milkyielding Holstein cows housed under the same husbandry and feeding conditions. All had calved at least once between the ages of 2.5 and 6 years, and were between 90 and 220 days in milk. In accordance with the farm’s reproductive management approach, a controlled internal drug release (CIDR) device was inserted into the vagina concurrent with a GnRH injection (day 0), and the device was removed …

Age-Related Estimates Of Genetic Parameters For Growth And Ultrasonic M. Longissimus Thoracis Et Lumborum Muscle Traits In Eşme Lambs, İbrahi̇m Cemal, Orhan Karaca, Onur Yilmaz, Nezi̇h Ata

Turkish Journal of Veterinary & Animal Sciences

The growth performance and ultrasonic M. longissimus thoracis et lumborum (LTL) muscle traits of 2397 Eşme lambs raised in the Eşme district of Uşak province, an important lamb production area in Western Anatolia, were evaluated at 3 months (n = 1690) and 5 months (n = 707) of age. The average age of the lambs at the time of the first measurement (at weaning) was 100.15 days, and 152.37 days at the second. At 3 months of age, the average live weight (LW) was 29.34 kg and the average daily gain (ADG) was 248.35 g/day. By month 5, the LW …

Housing System And Gender Effect On Carcass And Meat Quality Characteristics In Turkish Pekin Ducks, Hasan Önder, Uğur Şen, Mi̇ne Yilmaz, Si̇bel Bozkurt, Eli̇f Ci̇lavdaroğlu, Di̇lek Gökçek, Ahmet Uçar, Kadi̇r Erensoy, Burcu Kurnaz

Turkish Journal of Veterinary & Animal Sciences

Improving meat quality is one of the most important aims for scientists and breeders. This study aimed to evaluate the effects of housing system (indoor vs. free-range) and gender on carcass and meat quality traits in Pekin ducks. The animal material consisted of 20 male and 20 female birds in each housing system. Our results show cold carcass weights of 3174.10 ± 80.65 g and 2672.60 ± 59.63 g for male and female birds in the indoor housing system and 2821.10 ± 50.53 g and 2306.90 ± 43.32 g for male and female birds in the free-range housing system. The …

Effects Of Preslaughter Fasting On Ph And Bacterial Contamination In Akkaraman And Anatolian Merino Sheep, Sai̇m Boztepe, Özcan Şahi̇n, Talha Demi̇rci̇, Gi̇zem Coşkun, İbrahi̇m Ayteki̇n, İsmai̇l Keski̇n

Turkish Journal of Veterinary & Animal Sciences

The present study investigates the effects of the preslaughter fasting on pH and bacterial contamination in Akkaraman and Anatolian Merino sheep. Included in the study were 10 Akkaraman and 10 Anatolian Merino sheep (n = 20) that were fasted for 24 h prior to slaughter, and a further 10 Akkaraman and 10 Anatolian Merino sheep (n = 20) that continued to be fed until slaughter as a control group. The total mesophilic aerobic bacteria (TMAB) measured at 0 and 24 h in the fasting Akkaraman and Anatolian Merino sheep were 4.87/4.79 and 4.75/5.57 log CFU/g, respectively, and the corresponding Enterobacteriaceae …

The Effects Of Sodium Perborate Tetrahydrate On Digestibility And Nutrient Transporters In Rats, Büşra Özmen, Emre Şahi̇n, Muhammed Tokmak, Veysi̇ Kayri̇, Ramazan Özmen, Kazi̇m Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

Boron (B) is an essential micronutrient involved in hormone activity, energy metabolism, antioxidant defense, bone development, wound healing, and immune function. The present study explores the effects of sodium perborate tetrahydrate (SPT) on nutrient digestibility and the modulation of specific nutrient transporters in the jejunum and ileum of rats. A total of 40 rats were divided into four equal groups: 1) control, 2) SPT 0.5 (0.5 mg B/kg body weight [BW]), 3) SPT 1 (1 mg B/kg BW), and 4) SPT 2 (2 mg B/kg BW), and were fed the respective diets for a period of 4 weeks. The digestibility …

Morphology And Histology Of The Developing Ovary In Malaysian Village Chicken (Gallus Gallus Domesticus) Chicks, Nur Athirah Lope Abdul Rashid, Mazlina Mazlan, Intan Shameha Abdul Razak, Rozaini Mohd Zohdi, Awang Hazmi Awang Junaidi

Turkish Journal of Veterinary & Animal Sciences

The Malaysian village chicken (MVC; Gallus gallus domesticus) is an indigenous breed derived from the red junglefowl (Gallus gallus), exhibiting slower growth and reproductive rates. The MVC plays an important ecological role in local farming systems and represents a valuable genetic resource. However, detailed information on ovarian development in this breed remains limited. This study provides the first comprehensive morphological and histological characterization of the developing ovary in MVC chicks. A total of 20 chicks aged 1, 2, 4, and 8 weeks (n = 5 per age group) were examined. These MVC chicks possessed a single functional left ovary positioned …

Effect Of Different Pellet Binders On Palm Kernel On Growth Performance And Nutrient Digestibility In Broiler Chicken, Nuun Marfuah, Burhanudin Sundu, Rusdi Rusdi, Sugiarto Sugiarto

Jurnal Ilmu Ternak Universitas Padjadjaran

Feed plays a paramount role in supporting growth, metabolism, and production performance. Their high price encourages the utilization of inexpensive and alternative feed ingredients. PKM, one such feed ingredient, is produced by palm oil industries. However, there is still a very limited application because of its high crude fiber content, which needs processing technologies like pelletization with binder addition. Based on this background, this experiment has been conducted to study the effect of various types of natural and commercial binders in 15% PKM-based pellets on performance and nutrient digestibility of broilers. The study was done for five weeks using 150 …

Evaluating The Impact Of K135t Mutation On Malate Dehydrogenase Kinetic Activity, Emily Frost, Kaitlyn Mello

Undergraduate Research Symposium (2026- )

Malate Dehydrogenase (MDH) is a key protein in the citric acid cycle; its dysregulation can have catastrophic impacts, such as neurological and metabolic disorders, and various cancers. MDH catalyzes the reversible oxidation-reduction reaction between malate and oxaloacetate, also reducing NAD+ to NADH in the process. The citric acid cycle is one of the central metabolic pathways, connecting to other essential pathways, such as amino acid and fatty acid biosynthesis, highlighting the importance of understanding how MDH works. A key site in MDH is the flexible loop region (amino acids 119-137), responsible for substrate binding, catalysis, and orientation. The flexibility of …

Characterizing The Macroinvertebrate Community In Crissy Lake, An Impounded Reach Of The Pomme De Terre River, Prior To Channel Modification, Max Lundgren

Undergraduate Research Symposium (2026- )

The aging Crissy Lake Dam, on the Pomme de Terre River in Morris, has altered natural stream flow and impeded upstream passage of several fish species since 1939. In late 2026 the Minnesota Department of Natural Resources will replace the dam with stone arch rapids and facilitate river channel formation upstream of the dam. In order to evaluate the river modification project, we characterized the macroinvertebrate community in Crissy Lake, the impoundment upstream of the dam before implementation of channel modification. In September 2025 we collected benthic macroinvertebrates from two sets of Ekman grab samples. We found 12 taxa (average …

Proline To Serine Mutation In Flexible Loop Region Of Malate Dehydrogenase, Anna Molstad, Hannah Robertson

Undergraduate Research Symposium (2026- )

Malate Dehydrogenase (MDH) is an essential enzyme that converts malate to oxaloacetate using the reduction of NAD+ to NADH. In humans, MDH plays a role in energy metabolism by catalyzing a key step of the citric acid cycle, important for energy production. Other indirect roles for MDH include gluconeogenesis and reoxidizing NADH from fatty-acid β-oxidation. An important feature of MDH is a flexible loop region that can alter substrate binding and enzyme activity, but its role is not well understood. Our study investigated how Pro123 to Ser (P123S) mutation in the flexible loop region affects MDH function. We hypothesized that …

Evaluating Differences In The Dynamic Growth Patterns Of Cvi And Ler Genetic Strains Of Arabidopsis During Seedling Establishment, Emily Frost

Undergraduate Research Symposium (2026- )

Seedling establishment is the process by which seedlings transition from depending on seed nutrients to making their own through photosynthesis. An important aspect is photomorphogenesis, the growth of plants in response to light, transitioning from rapid embryonic stem growth to a slower rate focused on root growth and leaf formation. The process is influenced by many genetic and environmental factors, making better characterization essential to understand plant development. For our research, we examined the dynamic growth of two Arabidopsis genetic strains (Cvi and Ler) over time in response to darkness, then to the change in response when light is introduced. …

Biophysical Insights Into A Cryptic, Hydrophobic Binding Site On Pcna, Alexis Dispensa

Theses and Dissertations

Proteins are dynamic molecules whose functional landscapes extend beyond static structural representations. Transient cryptic pockets (buried cavities that emerge through protein motion) represent underexplored targets for allosteric regulation and drug discovery. General anesthetics are small, lipophilic, weakly polar molecules that are well suited to partition into such hydrophobic sites. In this work, environment-sensitive fluorescence, photoaffinity labeling, mass spectrometry, mutational analysis, and microsecond molecular dynamics simulations were integrated to discover and characterize a cryptic, highly apolar binding site within the hydrophobic core of human proliferating cell nuclear antigen (PCNA), a central scaffold in DNA replication and repair. Using the solvatochromic fluorescent …

The Role Of Pten Subcellular Localization In Neuronal Development And Autism Spectrum Disorder, Nicole Desmet

Dartmouth College Ph.D Dissertations

Autism spectrum disorders (ASD) are genetically heterogeneous neurodevelopmental conditions that nevertheless converge on altered neuronal connectivity and circuit function. A central unresolved problem in the field is how diverse genetic perturbations, acting at different biological levels and developmental stages, give rise to shared neurodevelopmental outcomes. This dissertation addresses this problem by examining how gene function, subcellular signaling, and developmental context interact to shape neuronal morphology and circuit-relevant phenotypes.

Cell-autonomous mechanisms regulating neuronal morphology are examined through the ASD risk gene Phosphatase and Tensin Homolog (PTEN) in developing dentate gyrus granule neurons. Selective biasing of PTEN to defined intracellular compartments in …

Growing Healthy Habits: Impacts Of A Youth Community Garden For Children In Rural Mississippi, Olivia G. Degravelle

Honors Theses

This study examines the implementation and outcomes of a Youth Garden Program conducted in Pontotoc, Mississippi. Funded by the Allstate Community Service Project Grant, the Youth Garden was constructed within the previously established Pontotoc Community Garden. The initiative comprised four weekly workshops designed for participants ranging from preschool to adolescence. Each session incorporated hands-on fruit and vegetable (FV) planting or harvesting, an educational lesson, and a complementary craft activity. Children’s FV consumption patterns, nutritional knowledge, and attitudes toward FV were assessed through pre- and post-program surveys. In addition, the photo-elicitation interview method invited participants to take digital photographs of garden …

Evaluating Sand Characteristics And Their Influence On Sea Turtle Nest Incubation Conditions, Thomas Naylor

Honors Theses

The embryonic development of sea turtles is strongly influenced by the environmental factors experienced during incubation within the nest. These environmental factors can influence hatchling sex ratios, body condition, and their success emerging from nests. Adult female sea turtles lay nests across a range of sites throughout the nesting season, resulting in each nest encountering unique abiotic conditions. This study aims to identify how variation in sand characteristics, such as grain size proportions, permeability, and color, influences nest temperature and subsequent hatchling outcomes of Loggerhead Sea Turtles (Caretta caretta) and Green Sea Turtles (Chelonia mydas) from …

Exploring Undergraduate Student’S Perceptions Of Agriculture, Cayden J. Barton

Honors Theses

Americans today, both young and old, face issues with agricultural knowledge regarding the food and fiber industry and production agriculture as a whole (Frick et al., 1995). In a society where consumers are separated from production agriculture, public interaction with agriculture is less prevalent and the importance of agriculture is undervalued. A person’s knowledge of agriculture, or agricultural literacy affects the way that they perceive agricultural commodities and the agricultural industry (Specht, McKim, & Rutherford, 2014). Without adequate knowledge of the agricultural industry consumers cannot make well informed decisions about food safety or processing methods. In addition to consumers, government …

Socially Constructing ‘Climate Change’ In Fishing Communities Of Sarangani, Philippines, Samantha Geraldine G. De Los Santos, Ma. Larissa Lelu P. Gata

Journal of Public Affairs and Development

The intensifying impacts of climate change have become more evident in recent years. While policies and programs have been implemented in the Philippines for decades, questions remain about how climate change is understood and experienced by fishing communities, the country’s poorest sector. By applying the Ecological-Symbolic Approach, this paper inquired into the fisherfolk’s social definition of climate change. A Qualitatively Driven Sequential Explanatory (QualDSE) mixed-methods design guided the study in developing an informed, substantiated, and context-based definition of climate change. This definition was also analyzed vis-a-vis the widely held definitions in national climate policy.

The terminology “climate change” remains unknown …

The Impact Of Sex Education On College Student’S Perceptions Of Sexual Taboos, Noel Gamon

Honors Theses

The purpose of this research is to explore how college students' differing levels of sex education are associated with their views about sexual behavior, particularly behaviors defined as taboo. In comparison to 25 years ago, adolescents are less likely to receive important sex education today; and are unlikely to receive valuable information until after their first sexual experience. Conventionally, the benefits of a sex education program are linked with its ability to lower STI rates, unplanned pregnancies, unwanted behavior, etc. Sexual taboos are sexual practices excluded from the hegemonic heterosexual monogamous relationship model. Presently, society acknowledges a wider range of …

G1p3 Dysregulates Interferon-Stimulated Gene Expression In Er (+) Breast Cancer Cells, Karina Santana

Honors Theses

Even with advances in diagnosis and treatment, breast cancer remains the second leading cause of cancer-related death in women, with nearly 90% of fatalities linked to drug resistance driven by metastasis and recurrence. G1P3, an anti-apoptotic mitochondrial protein, is upregulated in breast cancer and has been associated with increased metastatic potential and poor prognosis. Recent findings show that G1P3 enhances interactions between Rab5-containing endosomes (RCEs) and mitochondria, and emerging evidence suggests that mitochondria play a critical role in initiating interferon (IFN) responses. Based on this, we hypothesized that G1P3-mediated interactions between RCEs and mitochondria promote interferon-stimulated gene (ISG) expression in …