Life Sciences Commons^™

A Geothermal Amoeba Sets A New Upper Temperature Limit For Eukaryotes, H. Beryl Rappaport, Natalie A. Petek-Seoane, Tomas Tyml, Jessica K. Niblo, Felix Mikus, Naomi E. Gilbert, Kurt Labutti, Godwin Ani, Ethan Macvicar, Rachel M. Shepherd, Ignacio De La Higuera, Samuel J. Lord, Gautam Dey, Kenneth M. Stedman, Multiple Additional Authors

Biology Faculty Publications and Presentations

The study of temperature limits has transformed our understanding of life’s boundaries but has focused on bacteria and archaea. For decades after their discovery, no eukaryote was shown to replicate above 60◦C. We isolated a geothermal amoeba, Incendiamoeba cascadensis, that divides at 63◦C and is motile up to 64◦C. We identified an enrichment of genes related to proteostasis and environmental sensing relative to mesophilic amoebae. Comparative RNA sequencing of I. cascadensis revealed upregulation of pathways related to proteostasis, DNA repair, and membrane trafficking at high temperatures. Predicted proteins of I. cascadensis have distinct biophysical properties, including enrichment of positively charged …

Growing South Dakota (Fall 2026), College Of Agriculture, Food And, Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Support from donors helping set SDSU dairy graduates above the rest
[Page] 4 A decade of impact: Celebrating 10 years of cow-calf, swine education and research facilitates
[Page] 8 98-year-old donor keeps decades-long scholarship going in honor of late husband
[Page] 11 Looking back at Michele Dudash's tenure in academia
[Page] 12Giving students opportunities, experience to build their futures
[Page] 16 A Jackrabbit's journey in growing agricultural leadership
[Page] 18 Building South Dakota's next generation of rural veterinarians
[Page] 19 Conservation: A family affair
[Page] 20 South Dakota Water Resources Institute finds solutions to South Dakota's water challenges …

Beyond Nutrients: Frass Fertilizer Legacy Effects For Sustainable Nematode Management In Potato–African Nightshade Rotation Systems, Emmanuel O. Anedo, Dennis Beesigamukama, Benson Mochoge, Nicholas K. Korir, Solveig Haukeland, Xavier Cheseto, Moses Nyongesa, Patrick Pwaipwai, Sevgan Subramanian, Abdou Tenkouano, Chrysantus M. Tanga

All Peer-Reviewed Publications

The utilization of insect frass fertilizer in crop production systems has recently gained significant global interest, but there has been limited evidence of its residual benefits in rotational cropping systems. The legacy effects of black soldier frass fertilizers (BSFFF), conventional organic fertilizer (SAFI), inorganic fertilizer (NPK), synthetic nematicide, and their combinations on potato cyst nematode (PCN) management under potato-African nightshade rotational farming systems were investigated. Eight treatments were evaluated: BSFFF, SAFI, chitin-fortified BSFFF (BSFFF+5chitin), NPK + nematicide, combination of BSFFF and NPK so that each supplies 50% of the nitrogen required by potato (50BSFFF+50NPK), combination of SAFI and NPK so …

Grains, Seeds And Hay Industry Funding Scheme: 2026-29 Strategic Plan, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

Industry Funding Schemes (IFS) are a powerful effort fuelled by industry support and steered by industry stakeholders. The Grains, Seeds and Hay IFS addresses pest and disease threats that pose a high risk to profitability or production into the grains, seed and hay industry in Western Australia.

The Grains, Seed and Hay IFS is managed by an Industry Management Committee, composed of dedicated producers and growers. As a crucial link between industry and government, our IFS collaborates closely with the Department of Primary Industries and Regional Development (DPIRD) who manage the BAM regulation list and deliver impactful programs.

Parents Don’T Dictate The Menu: Lack Of Strong Host-Specific Parental Effects May Help Maintain Dietary Generalism, Emma J. Sellers, Mayra C. Vidal, Dhaval K. Vyas, Mykaela Tanino-Springsteen, Gina M. Wimp, Mattheau S. Comerford, Mariana Abarca, Shannon M. Murphy

Biological Sciences: Faculty Publications

The environment or experiences of a parent generation can impact the fitness of the next generation, a phenomenon known as parental effects. While a high-quality diet can directly benefit an individual and a low-quality diet can reduce fitness, a parental effect occurs only when there is a transgenerational response to these stimuli that manifests in the individual’s offspring. In dietary generalists, a parental effect could play an important role for offspring performance if the species experiences considerable variation in quality across host plants; posi- tive parental effects may result from host matching between parents and offspring on high-quality host plants, …

Silverleaf Nightshade (Solanum Elaeagnifolium) Undergoes Guild-Specific Transcriptomic Reprogramming Against Chewing Versus Piercing Sucking Herbivory, Arunsaikumar Karrem, Matthew Terry, Jagdish Jaba, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences Faculty Publications

Solanum elaeagnifolium (silverleaf nightshade, SLN) is a globally invasive perennial weed with reproductive plasticity and complex defence systems that significantly impact crop yields by invading fields of staple, commercial and ornamental crops. Despite being a host to multiple insect herbivores, the molecular programming enabling its rapid adaptation to defend against diverse feeding guilds remains poorly resolved. Here, we report a comprehensive de novo transcriptomic assembly and comparative analysis of S. elaeagnifolium challenged by two distinct herbivores: the piercing-sucking generalist cowpea aphid, Aphis craccivora and the chewing specialist tobacco hornworm, Manduca sexta. Differential gene expression analysis revealed a massive difference …

Exploring The Significance Of Kenya's Indigenous And Emerging Blue Foods For Sustainable Aquaculture Development. A Review, Jonathan Munguti, Mavindu Muthoka, Domitila Kyule, Esther Magondu, Kevin Obiero, Jimmy Mboya, Menaga Meenakshisundaram, Tanga M. Chrysantus, Gerald Kwikiriza, Francis K. Njonge

All Peer-Reviewed Publications

Kenya's aquaculture sector is at a pivotal stage, transitioning from a historical reliance on Nile tilapia and African catfish toward a more diversified production portfolio that integrates indigenous and emerging freshwater and marine species. This shift is increasingly recognized as essential to enhance climate resilience, food security, and ecosystem health. This systematic review synthesizes more than two decades (2000 to 31st January, 2026) of peer-reviewed literature to evaluate the biological suitability, culture performance, and socio-ecological roles of underutilized native taxa, including Ningu (Labeo victorianus), Jipe tilapia (Oreochromis jipe), freshwater prawns (Macrobrachium spp.), rabbitfish (Siganus sutor), marine-acclimatized Nile tilapia, mud crab …

Keys To The Families Of Vascular Plants In Northwestern California, James P. Smith Jr, John O. Sawyer Jr

Botanical Studies

No abstract provided.

A Checklist Of Vascular Plants Endemic To California, James P. Smith Jr

Botanical Studies

No abstract provided.

An Annotated List Of The Native And Naturalized Grasses Of Hawaii, James P. Smith Jr

Botanical Studies

No abstract provided.

The Grasses Of Oklahoma, James P. Smith Jr

Botanical Studies

No abstract provided.

Poisonous And Mechanically Injurious Grasses, James P. Smith Jr

Botanical Studies

No abstract provided.

Resource Assessment Report No. 10: Southern Nearshore And Estuarine Scalefish Resource: Sandy Sprat (Whitebait) 2026 Assessment, Daniel E. Yeoh, Brett Crisafulli, Rubie Evans-Powell, Sybrand A. Hesp

Resource Assessment Reports

Sandy sprat Hyperlophus vittatus (‘whitebait’) is a small-bodied clupeid which occurs in temperate nearshore marine and estuarine waters throughout southern Australia. In Western Australia, this species is almost exclusively caught by the commercial sector on the lower west coast, between Perth and Geographe Bay, where it is considered to comprise a single biological stock. Since 2010, sandy sprat has primarily been caught by the South West Beach Net Fishery (SWBN) in the Bunbury region. This is a shore-based fishery which utilises beach seine nets to target a variety of finfish species in shallow nearshore waters.

Commercial catch is monitored and …

Multi-Omic Characterization Of The Hispanic/Latino Blood Lipidome Reveals An Additional Locus And Attenuated Genetic Prediction, Wanying Zhu, Hung-Hsin Chen, Heather M. Highland, Corey Giles, Peter J. Meikle, Joanne Curran, Joseph B. Mccormick, Susan P. Fisher-Hoch, Kari E. North

Human Genetics Publications

While lipids have been extensively investigated, genetic regulation of the circulating lipidome in diverse populations remains poorly understood. We conducted a lipidome-wide genome-wide association study (GWAS) of 830 lipid species in 2,287 Hispanic/Latino participants and performed predictive modeling across omics layers. We identified 7,593 genome-wide significant SNPs mapping to 208 genes. Conditional analysis disentangled the long-range linkage disequilibrium artifacts from the pleiotropic FADS1/2/3 cluster. Separately, we discovered an association at the GPLD1 locus for a circulating ceramide. Colocalization revealed shared genetic architecture with conventional lipids alongside distinct, species-specific pathways. Incorporating Native/Indigenous American expression quantitative trait loci …

Structural Attributes Of Dominant Seagrass Species Modulate Invertebrate Assemblages Within The Shark Bay World Heritage Area, Inês Leal, Daniel Gorman, Lauren Mukheibir, Keisha Desmond, Simone Strydom

Research outputs 2022 to 2026

The structural complexity of seagrass meadows plays a central role in shaping the diversity and composition of associated invertebrate assemblages. In the Shark Bay World Heritage Area of Western Australia, the dominant species Amphibolis antarctica and Posidonia australis differ markedly in their above- and below-ground morphology, yet the invertebrate assemblages they support remain poorly characterised. This study provides the first contemporary assessment of these habitat–invertebrate relationships, a timely contribution given the region’s increasing exposure to frequent and extreme climatic events. We identified 13,124 invertebrates from 7 phyla, 16 classes and 55 orders/families and highlight marked differences between epifaunal assemblage structure …

Risks Of Mercury Release From Disturbance Of Blue Carbon Ecosystems, Fan Zhang, Jingrou Hu, Ana Carolina Ruiz-Fernández, Christian J. Sanders, Xinxin Li, Ruotong Chen, Kunshan Bao, Bigyan Neupane, Aidah Baloch, Yanhui Zheng, Oscar Serrano

Research outputs 2022 to 2026

Blue carbon ecosystems (BCEs) function as dual repositories of organic carbon and legacy contaminants. While BCEs are recognized carbon sinks, their role as mercury (Hg) sinks and the risk of their conversion into sources due to human and climatic disturbances remain unexplored. This review synthesizes current knowledge on the drivers and risks of contaminant remobilization from threatened BCEs, with a primary focus on mercury. We explore how natural and human disturbances influence Hg storage, release, and bioavailability, while evaluating the risks posed to ecosystems and human health. Through a three-level meta-analysis, we show that physical disturbance remobilizes approximately 54% (95% …

Understanding Ecosystem Responses To Recurrent Marine Heatwaves, Emily Lester, Stanley Mastrantonis, Sharyn M. Hickey, Anna Lafratta, Jessica A. Benthuysen, Eben Afrifa-Yamoah, Arani Chandrapavan, Martial Depczynski, Richard D. Evans, David V. Fairclough, Christopher J. Fulton, Marthe Monique Gagnon, Nicole L. Jones, Sina Pinter, Claire L. Ross, William D. Robbins, Zoe T. Richards, Karina L. Ryan, Simone Strydom, James R. Tweedley, Fred Wells, Hollie Williams, Shaun K. Wilson, Kathryn Mcmahon

Research outputs 2022 to 2026

Marine heatwaves (MHWs) have emerged as an increasingly severe disturbance to global marine ecosystems, with documented intensifications in both frequency and magnitude over the past century. Western Australia has experienced some of the most severe MHWs on record, causing widespread ecological impacts, including coral bleaching and mortality, local species extinctions, and socioeconomic impacts such as fishery closures, making the region a critical case study of climate-driven change in the marine domain. Most recently, a severe MHW during summer 2024/2025 has caused widespread disruption to marine life, reinforcing the region's vulnerability to repeated extreme events. While extensive research has documented the …

Aleurone Layer Multi-Omics Reveals Constitutive Enzyme-Inhibitor Imbalance In Lma-Contrasting Wheat Cultivars, Utpal Bose, Angéla Juhász, Nicolas Daneri-Castro, Shoko Okada, James A. Broadbent, Keren Byrne, Sally Stockwell, Ronald Yu, Jean-Philippe Ral, Michelle L. Colgrave

Research outputs 2022 to 2026

Late maturity amylase (LMA) is a genetic defect in wheat causing premature accumulation of high-pI alpha-amylase isoforms in the aleurone during grain development, which reduces falling number, resulting in flour downgrading and price penalties. Yet, the aleurone layer proteome and metabolome remain poorly characterised across LMA-contrasting cultivars. We measured the proteome and central carbon metabolome of aleurone from three wheat cultivars differing in LMA-phenotype: Seri (susceptible), Kennedy (intermediate), and Hartog (resistant) – under abscisic acid (ABA), abscisic acid plus gibberellic acid (GA), or untreated conditions. Cultivar identity explained 65.7% of proteome variance, exceeding hormone treatment effects (13.1%), with no significant …

Genomic Vulnerability Of A Threatened Possum In A Biodiversity And Climate Change Hotspot, Grace I. Marsh, Sean James Buckley, Daniel J. White, Adrian F. Wayne, Bryony J. Palmer, Nicola J. Mitchell

Research outputs 2022 to 2026

Spatial variation in the magnitude and pace of climate change impacts means some species will be more affected than others. This includes those occurring within global biodiversity hotspots, regions with exceptional concentrations of endemic species that have often been shaped by long-term climatic stability. Disproportionate levels of human-induced pressure in these areas are threatening much of the biodiversity values that make these biodiversity hotspots exceptional. Southwest Western Australia is a highly fragmented temperate biodiversity hotspot experiencing rapid climate change and supporting many species of threatened mammals. One Critically Endangered arboreal folivore, the western ringtail possum (ngwayir; Pseudocheirus occidentalis), has contracted …

Implementation Of The Problem-Based Learning Model On The Topic Of The Immune System To Enhance The Critical Thinking Skills Of Grade Xi B Students At Sman 1 Tojo, Nani Sofyanti, Sutrisnawati Sutrisnawati, Vita Indri Febriani, Mohammad Jamhari, Bustamin Bustamin, Raya Agni

Jurnal Pendidikan Sains

Critical-thinking skills are essential competencies for students in 21st-century learning. Preliminary classroom observations in Class XI B at SMAN 1 Tojo indicated that instruction remained predominantly teacher-centered and provided limited opportunities for students to engage in activities involving interpretation, analysis, evaluation, and inference. This study aimed to improve students’ critical-thinking skills through the implementation of Problem-Based Learning (PBL) on the immune system topic. The study employed a Classroom Action Research (CAR) design conducted over two cycles, involving 28 students in Class XI B. Data were collected using a teacher activity observation sheet and critical-thinking skills tests assessing four indicators: interpretation, …

Junior High School Students’ Understanding Of Pathogenic Microbes In The School Environment: A Cross-Grade Descriptive Study At Smp Alkhairaat 1 Palu, Feren Febrianti, I Nengah Kundera, Yulia Windarsih, Mohammad Jamhari, Mursito S. Bialangi, Abdul Ashari

Jurnal Pendidikan Sains

This study aimed to describe students’ understanding of pathogenic microbes and compare descriptive comprehension profiles across Grades VII, VIII, and IX at SMP Alkhairaat 1 Palu. A quantitative descriptive approach was employed involving 49 students selected through purposive sampling. Data were collected using an essay test as the primary instrument, supported by semi-structured interviews and observations of the school environment. The essay test assessed three indicators: conceptual knowledge of pathogenic microbes, understanding of disease transmission mechanisms, and understanding of clean and healthy practices for preventing the transmission of pathogenic microbes. Test data were analyzed descriptively using mean percentage scores, while …

The Ability Of Biology Teachers Of State Madrasah Aliyah In Palu City In Developing Teaching Modules In Merdeka Curriculum, Ulya Afifa, Gamar B. N Shamdas, Syech Zainal, Bustamin Bustamin, Raya Agni, Rafiqa Rafiqa

Jurnal Pendidikan Sains

Teachers’ ability to develop teaching modules is essential for implementing the Merdeka Curriculum because it enables curriculum expectations to be translated into flexible and context-responsive learning. This study aimed to describe Biology teachers’ ability to develop teaching modules at State Islamic Senior High Schools (Madrasah Aliyah Negeri [MAN]) in Palu City and to identify the factors supporting or constraining this ability. A descriptive qualitative study was conducted with seven Biology teachers from MAN 1 Palu, MAN 2 Palu, and MAN Insan Cendekia Palu during February–March 2026. Data were collected through questionnaires, interviews, documentation, and analysis of teaching modules using …

Obsessive-Compulsive Subtraits And Self-Agency, Jacob Nelson, Cody Barton, Carissa Phillipi Phd

Undergraduate Research Symposium

Obsessive-Compulsive Disorder (OCD) is categorized by persistent, unwanted thoughts, called obsessions. and repetitive behaviors, called compulsions. The Obsessive-Compulsive Inventory-Revised (OCI-R) is a valid measure of OCD, and breaks the condition into six subtraits: Checking, Hoarding, Obsessing, Ordering, Neutralizing, & Washing. Prior research has suggested that one’s sense of agency, measured through the Sense of Agency Scale (SoAS), is lower in those with higher overall OCI-R scores compared to those with lower scores and controls. Additionally, sub-clinical checking has been negatively correlated with sense of agency, and sub-clinical hoarding and ordering have been positively correlated with sense of agency. However, there …

Phenotypic Variation Between Surface And Cave-Adapted Poeciliids And Its Effect On Metabolism, Karch Knipp, Michael Tobler, Soren Johnson

Undergraduate Research Symposium

Cave-adapted organisms often exhibit pronounced morphological changes relative to their surface counterparts, a phenomenon known as troglomorphy. In Poecilia mexicana (Atlantic molly), these differences include variation in coloration between surface and cave populations. Troglobitic organisms also typically exhibit reduced metabolic rates, likely as an adaptation to environments with low primary productivity. This makes P. mexicana an ideal system for investigating the evolution of metabolic traits. In this study, we leveraged variation in coloration among cave-dwelling mollies to examine potential associations with resting metabolic rate (RMR). Coloration was quantified using PCR values, and individuals were categorized as “yellow” or “non-yellow.” We …

The Impact Of Caffeine On Bumble Bee Hunger, Hallie Chapman, Becky Hansis-O'Neill, Aimee Dunlap Phd

Undergraduate Research Symposium

Bumble bees (Bombus impatiens) are crucial pollinators, responsible for pollinating multiple native plants and agricultural crops. Caffeine, which is classified as a stimulant, is present in some of these plant species at varying levels. Alterations in bumble bee cognition, including motivation, learning, and hunger, have been associated with caffeine intake. This study explored the impacts of caffeine on the bumble bee behavior on a biological level. While previous research has examined caffeine’s effects on cognition and learning, fewer studies have investigated its effects on hunger at the level of individual bees. Bumble bees, identified with numerically labeled tags, …

Caffeine Concentration: An Analysis On Bumble Bee Foraging Efficiency, Carson Leonard, Becky Hansis-O’Neill, Aimee Dunlap Phd

Undergraduate Research Symposium

Caffeine (trimethylxanthine) is a synthetically and naturally produced stimulant that has been shown to elicit an effect on the central nervous system of humans as well as invertebrates. Although the exact mechanisms by which caffeine alters biological processes in invertebrates are still being investigated, its physiological and behavioral effects have been extensively documented. Naturally occurring caffeine is produced by certain flowering plants, including cocoa and tea species, and has been shown to influence invertebrate behavior, particularly cognitive performance and foraging patterns. In pollinating insects, the exposure to caffeine in floral nectar may affect how pollinators evaluate, select, and revisit food …

Tarantulas Can Use Black And White Cues In A Discrimination Learning Task, Jordan Kolinski, Becky Hansis O'Neill, Amiee Dunlap

Undergraduate Research Symposium

Animals use vision to perceive light, shapes, colors, and movement. Many animals that have poor vision are understudied. The purpose of this experiment was to study color vision in tarantulas (Brachypelma hamorii). Researchers have found genes associated with color vision tarantulas, but no behavioral experiments have been done on this topic. Previous research has also shown that tarantulas can see polarized light. In this experiment, we wanted to know if tarantulas could use visual cues to move away from a noxious stimulus - vibration. There were twelve tarantulas being tested in this experiment for between twelve and fifteen …

Relationship Between Morphological And Behavioral Laterality In Astyanax Mexicanus, Allison Lendman, Michael Tobler, Erik Johnson

Undergraduate Research Symposium

Populations within the Astyanax genus have experienced extreme differences in environment, leading to distinct morphological adaptations. The cave populations of these fish are eyeless and have acquired fixed, cranial asymmetries. We explored a possible relationship between this physical asymmetry and behavioral laterality. We tested 28 adult fish, 14 male and 14 female, from the Pachón cave in controlled behavioral trials. We found that both sexes demonstrated behavioral laterality, observed as a consistent left-turn bias in these trials. This supports the hypothesis that these populations display behavioral laterality that is correlated to their physical asymmetries. Ongoing research comparing behavioral patterns in …

Under The Surface: Human Disturbance Reduces Species Richness In Glade Microhabitats, Maddie Smith, Becky Hansis-O'Neill, Aimee Dunlap Phd

Undergraduate Research Symposium

Human disturbance can alter microhabitats in ways that affect biodiversity, especially in sensitive ecosystems like glades where many animals rely on rocks for thermoregulation and refuge. This study examined how rock displacement by human activity influences species richness and community composition in glade habitats at Shaw Nature Reserve and Victoria Glades Conservation Area. We hypothesized that disturbed rocks would be less suitable for wildlife and would support fewer species. From September to November 2025, about 30 rocks per site were pseudo-randomly selected and monitored. Each rock was classified as displaced or nondisplaced, and all organisms found beneath them were recorded. …

Enantioselectivity Of The Photochemical Continuous-Flow Isomerization Of Humulones, Gregory M. Giovine, Trevor Smith, Bruce Hamper

Undergraduate Research Symposium

Humulones, the primary natural product present in the historically significant hop flower, represent a largely unexplored candidate for structure-based pharmaceutical design.  Showing promising biological activity as an anti-bacterial and anti-inflammatory agent, humulones are of interest both for their bioactive properties and for their long-established tolerability and natural abundance.  Humulones undergo a characteristic isomerization reaction, where the six-membered substituted phloroglucinol ring contracts to a five-membered ring isohumulone, under a variety of conditions, including those enabled by brewing conditions (thermal, metal-catalyzed) and by exposure to UV light.  Optimization of the photocatalyzed isomerization of n-humulone to n-isohumulone in flow conditions resulted in a …