Life Sciences Commons^™

The Contribution Of Outcome Expectation In Water Saving Among Malaysians For Sustainable Living Standard, Ganesh. R, Vxynette Fiorna, Ammar Abdulaziz Al-Talib, Haslinda. A, Elango Natarajan

ASEAN Journal on Science and Technology for Development

Saving water is an essential component of a higher level of living for each individual in any Nation. The current and next generation will be greatly impacted by attitudes and emotional aspects of social cognitive behaviour. This empirical investigation was carried out in Malaysia. To determine their attitude and behaviour, a convenience sampling method was applied to a sample size of 265 individuals. It was discovered that outcome expectancy significantly improves water saving. Observed self-efficacy, personal elements, and environmental elements all impacted the outcome expectancy. The model evaluation showed that the adjusted R2 was 0.78, or about 78% of the …

Pelletizing Of Attapulgite/Carbon Nanocomposite From Used Bleaching Earth For Continuous Treatment Of Wastewater From A Natural Rubber Factory, Lilis Hermida, Joni Agustian, Yin Fong Yeong

ASEAN Journal on Science and Technology for Development

The increase in global natural rubber production has correspondingly elevated the generation of wastewater. To address this, various wastewater treatment methods are being implemented, with continuous adsorption emerging as a promising approach. In this recent study, pelletizing of attapulgite/carbon nanocomposite was conducted to obtain pellet adsorbent for continuous adsorption of wastewater from natural rubber industry. The study aimed to evaluate the characteristics and performance of the pellet adsorbent in wastewater treatment. In the preparation, spent bleaching earth (SBE) with a 200-mesh particle size was calcined to obtain attapulgite/carbon nanocomposite. This nanocomposite was mixed with bentonite clay at a 70:30 (w/w) …

Enhancing Phishing Attack Detection Using Explainable Ai: Trends And Innovations, Bhagyashree D. Shendkar, Pankaj R. Chandre, Sulochana Sagar Madachane, Nikita Kulkarni, Sayalee Deshmukh

ASEAN Journal on Science and Technology for Development

In the context of escalating cyber threats, phishing attacks continue to pose significant challenges to cybersecurity measures, exploiting human vulnerabilities to gain unauthorized access to sensitive information. Innovative solutions are required because traditional detection methods frequently fail to identify and mitigate these assaults successfully. To improve phishing attack detection, this study makes use of Explainable Artificial Intelligence (AI) tools, which provide decision-making processes with transparency and prediction accuracy. The paper begins by outlining the pervasive nature of phishing attacks in today's digital landscape, emphasizing their detrimental impact on individuals, organizations, and society at large. It then discusses the limitations of …

Trends And Patterns Of Plant Conservation Research In Borneo: A Review, Rizmoon Nurul Zulkarnaen, Sabri Mohd. Ali, Daniele Cicuzza

ASEAN Journal on Science and Technology for Development

Borneo hosts exceptional plant diversity, including numerous endemic species, but faces significant threats from rapid habitat loss driven by deforestation and land-use changes. This study aimed to analyze research trends and forecast future directions through a bibliometric analysis of Scopus data spanning from 1914 to 2023. The bibliometric analysis included publication outputs, citation patterns, thematic mapping, and keyword co-occurrence. We used VOSviewer to visualize bibliometric networks and the Bibliometrix package in R to perform advanced bibliometric analysis and interactive data exploration. The analysis revealed a continuous rise in publications (998 documents), with the Journal of Ecology identified as the most …

Policy Strategies For Developing Superior Human Resources For The Sugar Cane Agro-Industry In The Industrial Era 4.0 In Indonesia, Mursiti Mursiti, Illah Sailah, Marimin Marimin, Muhammad Romli, Alex Denni

ASEAN Journal on Science and Technology for Development

The development of Industry 4.0 creates new challenges for human resources. Research in the field of human resources for the sugarcane agro-industry is still rare and is important because it is a strategic commodity in Indonesian for the sustainability of the sugarcane industry. The scope of the research ranges from the cut load transportation to sugar refining. The aim of the research is to conduct human resource scenario planning towards Human Resource 4.0 in sugarcane agroindustry. The research method used is scenario planning. The data were obtained from primary and secondary data. The variables used are human resource readiness and …

How Individuals’ Opinions Influence Society’S Resistance To Epidemics: An Agent-Based Model Approach, Geonsik Yu, Michael J. Garee, Mario Ventresca, Yuehwern Yih

Faculty Publications

Background: Protecting public health from infectious diseases often relies on the cooperation of citizens, especially when self-care interventions are the only viable tools for disease mitigation. Accordingly, social aspects related to public opinion have been studied in the context of the recent COVID-19 pandemic. However, a comprehensive understanding of the effects of opinion-related factors on disease spread still requires further exploration. Methods: We propose an agent-based simulation framework incorporating opinion dynamics within an epidemic model based on the assumption that mass media channels play a leading role in opinion dynamics. The model simulates how opinions about preventive interventions change over …

Research Report: Radicchio Cultivar Performance In New Hampshire, Rebecca G. Sideman, Ella Lukacz, Lilly Hartman

Faculty Publications

Radicchio (Cichorium intybus var. latifolium) is grown widely throughout Europe, especially northern Italy. In addition to having interesting color and flavor profiles, they are rich in phenolics and other bioactive compounds. While increasingly popular as a niche vegetable in the region, there is little research-based information to guide growers. The New England growing climate is very different from that of major production regions, and it presents unique challenges. There is tremendous phenotypic variation among and within groups and cultivars. The availability of varieties to commercial growers in the U.S. has expanded greatly, but there remains a need for reliable …

Identification And Characterization Of Two Novel Kcnh2 Mutations Contributing To Long Qt Syndrome, Anthony Owusu-Mensah, Jacqueline Treat, Joyce Bernardi, Ryan Pfeiffer, Robert Goodrow, Bright Tsevi, Victoria Lam, Michel Audette, Jonathan M. Cordeiro, Makarand Deo

Electrical & Computer Engineering Faculty Publications

We identified two different inherited mutations in KCNH2 gene, or human ether-a-go-go related gene (hERG), which are linked to Long QT Syndrome. The first mutation was in a 1-day-old infant, whereas the second was in a 14-year-old girl. The two KCNH2 mutations were transiently transfected into either human embryonic kidney (HEK) cells or human induced pluripotent stem-cell derived cardiomyocytes. We performed associated multiscale computer simulations to elucidate the arrhythmogenic potentials of the KCNH2 mutations. Genetic screening of the first and second index patients revealed a heterozygous missense mutation in KCNH2, resulting in an amino acid change (P632L) in the …

Methylene Blue-Mediated Photodynamic Therapy In Combination With Doxorubicin: A Novel Approach In The Treatment Of Ht-29 Colon Cancer Cells, Nima Rastegar-Pouyani, Jaber Zafari, Alireza Nasirpour, Hossein Vazini, Nabbaa Najjar, Seyedeh Zohreh Azarshin, Fatemeh Javani Jouni

Electrical & Computer Engineering Faculty Publications

Introduction: With an alarmingly growing number of patients diagnosed with colorectal cancer, adopting innovative anti-cancer approaches has recently garnered great attention. One interesting concept is the co-administration of cytotoxic agents and safer modalities such as photodynamic therapy (PDT), which can subsequently improve therapeutic efficacy and potentially reduce the risks of severe adverse effects and drug resistance. In the course of PDT, a locally injected photosensitizer (PS) is irradiated with a light source, which subsequently generates reactive oxygen species (ROS) and induces programmed cell death in tumor cells.

Methods: In this study, to evaluate the potential anti-cancer effects of chemotherapy combined …

An Overview Of The Relationships Between The Food Industry And Nanotechnology, Mehdi Koushki, Nasrin Amiri-Dashatan, Hossein Pourghadamyari, Hadi Khodabandehloo, Fatemeh Bagheri, Masoumeh Farahani, Lobat Tayebi

Electrical & Computer Engineering Faculty Publications

Background and Objective: Due to the growth of the global population, food demands are increasing. Hence, the need to develop more efficient methods for producing better quality, safer, and more sustainable food seems essential. In the past decades, the use of nanoscale materials has increased greatly due to the unique chemical, physical, and biological characteristics of nanomaterials compared to bulk materials. This research presents nanotechnology role in improving sensorial properties (taste, appearance, and texture) and safety aspects as well as processing and packaging of foods. The use of nano-omics-based technologies and artificial intelligence-nanotechnology-based technologies in the food industry is also …

Determining The Quality And Quantity Of California Sitka Spruce (Picea Sitchensis) On Private Commercial Timberland For Use In Domestic Acoustic Steel-String Guitars, Ryan Eugene Cooper

Undergraduate Scholarship

Development of additional revenue streams for timber products with relatively low profitability is an important step in securing future economic success for the forestry sector in Humboldt county. Utilization of California Sitka spruce (Picea sitchensis) for use in domestic acoustic steel-string guitar manufacturing may provide exactly that. Determining the quality and quantity of California Sitka spruce is the logical first step in achieving this.

Quality was determined from tree core samples gathered at sites on Green Diamond Resource Company’s (GDRco) California timberlands and the Arcata Community Forest (ACF) with high basal area of Sitka spruce. Rings Per Inch …

The Food And Habitat Effects Of Detrital Kelp As A Trophic Subsidy To Seagrass-Inhabiting Mesograzers, Jessica Billinghurst

Theses: Doctorates and Masters

Seascapes are connected through the transfer of energy from the movement of fauna, detritus, and nutrients among habitats. This exchange of resources can influence food web dynamics in the recipient system, increasing productivity and biodiversity, thus creating spatial subsidies. Spatial subsidies are usually characterised by the transfer of resources from highly productive donor systems to lower productive recipient systems. However, recent work suggests that recipient systems with comparably high productivity, such as seagrass meadows, may benefit from spatial subsidies. Kelp is regularly eroded from reefs and provides a vector for nutrient and detrital transfer to seagrass meadows, where it can …

Shifting Soil Fungal Communities In Response To Fire And Weed Management In Urban Banksia Woodlands, Aaron Justin Brace

Theses: Doctorates and Masters

This research aims to fill the knowledge gap in soil fungi in the Banksia woodland and emphasizes considering holistic ecosystem dynamics in invasive species management strategies. This is accomplished by surveying soil fungi under different fire regime across the Swan Coastal Plain, and within both in and ex situ herbicide application trials. This is the first such study to investigate the fungal component of the Banksia woodland so thoroughly. Chapter 2 quantified and analysed changes in soil fungal community composition and functional groups over a 50-year fire chronosequence in fire-prone Banksia woodland in southwestern Australia. Effects were variable, but fungal …

Irrigation Demand For Fruit Trees Under A Climate Change Scenario Using Artificial Intelligence: Demanda De Irrigação Para Frutíferas Sob Cenário De Mudanças Climáticas Utilizando-Se Inteligência Artificial, Rafael Battisti, Waldemiro Alcantara Da Silva Neto, Ronaldo Martins Da Costa, Felipe Puff Dapper, Elvis F. Elli

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Fruit growing, especially in family farming, has a significant income potential in small areas, but climate change is a major challenge. This study aimed to quantify the irrigation requirements for citrus, papaya, mango and passion fruit, in the Vão do Paranã region, Goiás state, Brazil. The climate data encompassed the observed periods from 1961 to 2020 and future scenarios from 2021 to 2100. The irrigation demand was obtained from the daily water balance, while the reference evapotranspiration (ETo) was estimated using the Penman-Monteith method and then compared with an artificial intelligence tool. The future scenarios indicated a higher increase for …

Multifactorial Exercise Intervention Decreases Falls Risk In High-Risk And Low-Risk Older Adults, Amanda Estep, Steven Morrison, Shane V. Caswell, Jatin P. Ambegaonkar, João R. Vaz, Nelson Cortes

Rehabilitation Sciences Faculty Publications

Background: Each year, 1 in 4 people over the age of 65 years of age will experience a fall. It is important to identify and address modifiable risk factors that are associated with falls in adults at high and low risk for falls.

Hypothesis: Falls risk improves in both high-risk and low-risk participants with the implementation of Stay Active and Independent for Life (SAIL).

Study Design: Cohort study.

Level of Evidence: Level 3.

Methods: Seventy-eight older adults (age, 70.9 ± 5.1 years) were included in this study and categorized into high risk and low risk for falling based on the …

Effect Of Task Constraints On Neurobiological Systems Involved In Postural Control In Individuals With And Without Chronic Ankle Instability, Yuki A. Sugimoto, Patrick O. Mckeon, Christopher K. Rhea, Carl G. Mattacola

Rehabilitation Sciences Faculty Publications

The purpose of this study is to investigate the effect of task constraints on the neurobiological systems while maintaining postural control under various sensory feedback manipulations in individuals with and without Chronic Ankle Instability (CAI). Forty-two physically active individuals, with and without CAI, were enrolled in a case-control study conducted at a biomechanics research laboratory. All participants underwent the Sensory Organization Test (SOT), which assesses individuals’ ability to integrate somatosensory, visual, and vestibular feedback to maintain postural control in double-, uninjured-, and injured-limb stances under six different conditions in which variations in the sway-referenced support surface (platform) and visual surroundings, …

Clothing Design Solutions For Children With Developmental Disabilities: A Scoping Review Protocol, Nagaveni N. Nayak, Shristi Shakya, Nachiket Gudi, Sonia Khurana, Sivakumar Gopalakrishnan, Veena Rao, Bhamini Krishna Rao

Rehabilitation Sciences Faculty Publications

Children with Developmental Disabilities (DD) often face significant challenges in performing dressing task, fundamental activities of daily living. Inability to accomplish this task independently significantly impacts participation in social and recreational activities, affecting Quality of Life (QoL). Additionally, burden on caregivers increases which in turn leads to stress and burnout. Addressing dressing-related challenges through innovative clothing designs enhances self-independence, alleviate caregiver burden, and improve QoL. However, studies published in this focused area are quite heterogeneous resulting in lack of comprehensive understanding of different clothing solutions that exist for children with DD. Therefore, this scoping review is undertaken to map clothing …

An Objective Assessment Of Neuromotor Control Using A Smartphone App After Repeated Subconcussive Blast Exposure, Charlend K. Howard, Masahiro Yamada, Marcia Dovel, Rie Leverett, Alexander Hill, Kenneth A. Manlapaz, David O. Keyser, Rene S. Hernandez, Sheilah S. Rowe, Walter S. Carr, Michael S. Roy, Christopher K. Rhea

Rehabilitation Sciences Faculty Publications

Subconcussive blast exposure has been shown to alter neurological functioning. However, the extent to which neurological dysfunction persists after blast exposure is unknown. This longitudinal study examined the potential short- and long-term effects of repeated subconcussive blast exposure on neuromotor performance from heavy weapons training in military personnel. A total of 214 participants were assessed; 137 were exposed to repeated subconcussive blasts and 77 were not exposed to blasts (controls). Participants completed a short stepping-in-place task while an Android smartphone app placed on their thigh recorded movement kinematics. We showed acute suppression of neuromotor variability 6 h after subconcussive blast …

Multimodel Approach To A Support Stock Assessment Of Standardized Catch And Effort Data: A Case Study Of Blue Shark (Prionace Glauca) In The Indian Ocean By The Taiwanese Large-Scale Longline Fishery, Chun-Yi Hung, Hoang Huy Huynh, Xing-Han Wu, Wen-Pei Tsai

Journal of Marine Science and Technology–Taiwan

In the context of stock assessment and fishery conservation, determining catch per unit effort (CPUE) accurately and reliably is essential. This study estimated trends in the relative abundance of blue sharks (Prionace glauca) in the Indian Ocean from 2005 to 2022. Blue sharks constitute a resilient bycatch species in pelagic tuna and swordfish longline fisheries. We employed a multimodel approach that included delta-lognormal, zero-inflated negative binomial, and vector autoregressive spatiotemporal (VAST) models to standardize catch rates recorded by observers in the Taiwanese large-scale longline fishery. Our analysis concentrated on the CPUE of blue sharks, including standardizing the number of fish …

Investigation Of Autosegmentation Techniques On T2-Weighted Mri For Off-Line Dose Reconstruction In Mr-Linac Workflow For Head And Neck Cancers, Brigid A Mcdonald, Carlos E Cardenas, Nicolette O'Connell, Sara Ahmed, Mohamed A Naser, Jiaofeng Xu, Raed J Zuhour, Shane Mesko, Alexander Augustyn, Samantha M Buszek, Stephen Grant, Bhavana V Chapman, Alexander F Bagley, Renjie He, Abdallah S R Mohamed, John Christodouleas, Kristy K Brock, Clifton D Fuller

Faculty, Staff and Student Publications

Background: In order to accurately accumulate delivered dose for head and neck cancer patients treated with the Adapt to Position workflow on the 1.5T magnetic resonance imaging (MRI)-linear accelerator (MR-linac), the low-resolution T2-weighted MRIs used for daily setup must be segmented to enable reconstruction of the delivered dose at each fraction.

Purpose: In this pilot study, we evaluate various autosegmentation methods for head and neck organs at risk (OARs) on on-board setup MRIs from the MR-linac for off-line reconstruction of delivered dose.

Methods: Seven OARs (parotid glands, submandibular glands, mandible, spinal cord, and brainstem) were contoured on 43 images by …

Protein-Centric Omics Analysis Reveals Circulating Complements Linked To Non-Viral Liver Diseases As Potential Therapeutic Targets, Yingzhou Shi, Hang Dong, Shiwei Sun, Xiaoqin Wu, Jiansong Fang, Junming Han, Huixiao Wu, Luna Liu, Wanhong Wu, Yang Tian, Guandou Yuan, Xiude Fan, Chao Xu

Faculty, Staff and Student Publications

BACKGROUND/AIMS: To evaluate the causal correlation between complement components and non-viral liver diseases and their potential use as druggable targets.

METHODS: We conducted Mendelian randomization (MR) to assess the causal role of circulating complements in the risk of non-viral liver diseases. A complement-centric protein interaction network was constructed to explore biological functions and identify potential therapeutic options.

RESULTS: In the MR analysis, genetically predicted levels of complement C1q C chain (C1QC) were positively associated with the risk of autoimmune hepatitis (odds ratio 1.125, 95% confidence interval 1.018-1.244), while complement factor H-related protein 5 (CFHR5) was positively associated with the risk …

A Relay Velocity Model Infers Cell-Dependent Rna Velocity, Shengyu Li, Pengzhi Zhang, Weiqing Chen, Lingqun Ye, Kristopher W Brannan, Jun-Ichi Abe, Guangyu Wang

Faculty, Staff and Student Publications

RNA velocity provides an approach for inferring cellular state transitions from single-cell RNA sequencing (scRNA-seq) data. Conventional RNA velocity models infer universal kinetics from all cells in an scRNA-seq experiment, resulting in unpredictable performance in experiments with multi-stage and/or multi-lineage transition of cell states where the assumption of the same kinetic rates for all cells no longer holds. Here we present cellDancer, a scalable deep neural network that locally infers velocity for each cell from its neighbors and then relays a series of local velocities to provide single-cell resolution inference of velocity kinetics. In the simulation benchmark, cellDancer shows robust …

Analysis Of B7-H4 Expression Across Salivary Gland Carcinomas Reveals Adenoid Cystic Carcinoma-Specific Prognostic Relevance, Juliana Mota Siqueira, Yoshitsugu Mitani, Camilla Oliveira Hoff, Flavia Bonini, Luana Guimaraes De Sousa, Anurag Purushothaman, Hui Dai, Shiaw-Yih Lin, Michael T Spiotto, Ehab Y Hanna, Daniel J Mcgrail, Adel K El-Naggar, Renata Ferrarotto

Faculty, Staff and Student Publications

B7-H4 (VTCN1), a member of the B7 family, is overexpressed in several types of cancer. Here we investigated the pattern of expression of B7-H4 in salivary gland carcinomas (SGC) and assessed its potential as a prognostic marker and therapeutic target. Immunohistochemistry (IHC) analyses were performed in a cohort of 340 patient tumors, composed of 124 adenoid cystic carcinomas (ACC), 107 salivary duct carcinomas (SDC), 64 acinic cell carcinomas, 36 mucoepidermoid carcinomas (MEC), 9 secretory carcinomas (SC), as well as 20 normal salivary glands (controls). B7-H4 expression was scored and categorized into negative (< 5% expression of any intensity), low (5%-70% expression of any intensity or >70% with weak intensity), or high ( …

Clic1-Mediated Autophagy Confers Resistance To Ddp In Gastric Cancer, Zhen-Liang Nong, Kun Zhao, Ye Wang, Zhu Yu, Cong-Jun Wang

Faculty, Staff and Student Publications

Gastric cancer has been a constant concern to researchers as one of the most common malignant tumors worldwide. The treatment options for gastric cancer include surgery, chemotherapy and traditional Chinese medicine. Chemotherapy is an effective treatment for patients with advanced gastric cancer. Cisplatin (DDP) has been approved as a critical chemotherapy drug to treat various kinds of solid tumors. Although DDP is an effective chemotherapeutic agent, many patients develop drug resistance during treatment, which has become a severe problem in clinical chemotherapy. This study aims to investigate the mechanism of DDP resistance in gastric cancer. The results show that intracellular …

Secondhand Tobacco Smoke Exposure In Homes And Vehicles In Youth: Disparities Among Racial, And Sexual And Gender Minorities, Rajesh Talluri, Sahil S Shete, Surendra S Shastri, Sanjay Shete

Faculty, Staff and Student Publications

BACKGROUND: Secondhand smoke exposure (SHSe) among youth is a serious public health concern, leading to an increased risk of conditions such as asthma and respiratory infections. However, there is little research on SHSe among vulnerable populations, such as racial and sexual minorities. Understanding the factors associated with youth SHSe in homes and vehicles is crucial to developing better protective policies.

METHODS: This study utilized 2020 data from the National Youth Tobacco Survey, a representative sample of middle- and high-school students in the US. The primary outcomes were youth SHSe at home and while riding in a vehicle. Multinomial regression models …

First Evidence Of Microplastic Inhalation Among Free-Ranging Small Cetaceans, Miranda K Dziobak, Andreas Fahlman, Randall S Wells, Ryan Takeshita, Cynthia Smith, John Weinstein

Faculty, Staff and Student Publications

Plastic is a ubiquitous environmental contaminant, resulting in widespread exposure across terrestrial and marine spaces. In the environment, plastics can degrade into microparticles where exposure has been documented in a variety of fauna at all trophic levels. Human epidemiological studies have found relationships between inhaled microplastics and oxidative stress and inflammation. Previous studies of bottlenose dolphins (Tursiops truncatus) have reported prevalent exposure to plasticizing chemicals (e.g., phthalates) as well as particle loads in gastrointestinal tracts, but exposure from inhalation has not yet been studied. The objective of this study was to determine if inhalation is a viable route of microplastic …

Combination Chemotherapy Optimization With Discrete Dosing, Temitayo Ajayi, Seyedmohammadhossein Hosseinian, Andrew J Schaefer, Clifton D Fuller

Faculty, Staff and Student Publications

Chemotherapy drug administration is a complex problem that often requires expensive clinical trials to evaluate potential regimens; one way to alleviate this burden and better inform future trials is to build reliable models for drug administration. This paper presents a mixed-integer program for combination chemotherapy (utilization of multiple drugs) optimization that incorporates various important operational constraints and, besides dose and concentration limits, controls treatment toxicity based on its effect on the count of white blood cells. To address the uncertainty of tumor heterogeneity, we also propose chance constraints that guarantee reaching an operable tumor size with a high probability in …

Polaris: A Phase 2 Trial Of Encorafenib Plus Binimetinib Evaluating High-Dose And Standard-Dose Regimens In Patients With Braf V600-Mutant Melanoma With Brain Metastasis, Alexander M Menzies, Georgina V Long, Amiee Kohn, Hussein Tawbi, Jeffrey Weber, Grant A Mcarthur, Yanina Pfluger, Karl Lewis, Katy K Tsai, Omid Hamid, Hans Prenen, Luis Fein, Erjian Wang, Carolin Guenzel, Fan Zhang, Joseph F Kleha, Alessandra Di Pietro, Michael A Davies

Faculty, Staff and Student Publications

Background: POLARIS (phase 2 [ph2]; NCT03911869) evaluated encorafenib (BRAF inhibitor) in combination with binimetinib (MEK1/2 inhibitor) in BRAF/MEK inhibitor-naïve patients with BRAF V600-mutant melanoma with asymptomatic brain metastases.

Methods: The safety lead-in (SLI) assessed tolerability for high-dose encorafenib 300 mg twice daily (BID) plus binimetinib 45 mg BID. If the high dose was tolerable in ph2, patients would be randomized to receive high or standard dose (encorafenib 450 mg once daily [QD] plus binimetinib 45 mg BID). Otherwise, standard dose was evaluated as the recommended ph2 dose (RP2D). Patients who tolerated standard dosing during Cycle 1 could be dose …

Spatial Multiplexing And Omics, Julienne L Carstens, Santhoshi N Krishnan, Arvind Rao, Anna G Sorace, Erin H Seeley, Jared K Burks

Faculty, Staff and Student Publications

Much like solving a game of Clue, understanding physiological mysteries involves answering who, what, when, and where. Multi-omics approaches delve into cellular and molecular identities (the who and what), while longitudinal data collection addresses the when. Spatial dimensions address the where. This Primer discusses current technologies enabling quantification across biological scales, emphasizing the importance of retaining the spatial dimension of that data. We outline experimental design considerations, including targeted versus untargeted approaches, sample types, biological scale, and four main classes of molecule detection. Spatial analytics are explored, covering questioning approaches, analytical platforms, image segmentation and sampling. Example applications, reproducibility considerations, …

Usp22 Overexpression Fails To Augment Tumor Formation In Mmtv-Erbb2 Mice But Loss Of Function Impacts Mmtv Promoter Activity, Xianghong Kuang, Andrew Salinger, Fernando Benavides, William J Muller, Sharon Y R Dent

Faculty, Staff and Student Publications

The Ubiquitin Specific Peptidase 22 (USP22), a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) histone modifying complex, is overexpressed in multiple human cancers, but how USP22 impacts tumorigenesis is not clear. We reported previously that Usp22 loss in mice impacts execution of several signaling pathways driven by growth factor receptors such as erythroblastic oncogene B b2 (ERBB2). To determine whether changes in USP22 expression affects ERBB2-driven tumorigenesis, we introduced conditional overexpression or deletion alleles of Usp22 into mice bearing the Mouse mammary tumor virus-Neu-Ires-Cre (MMTV-NIC) transgene, which drives both rat ERBB2/NEU expression and Cre recombinase activity from the MMTV promoter resulting …