Impact Of Caffeine On Participant Memory For A Stressful Experience And The Development Of Intrusive Memories, Jaycee L. Kirkland, Kaylina R. Mcclenen, Natalina E. Porter, Sarah M. Wood, Julia Gross, Madilyn E. Paphanchith, Kelsey M. Siereveld, Jasmine Ic Ifeakanwa, Caeden M. Gurganus, Koen N. Suzelis, Aiden J. Farrell, Boyd R. Rorabaugh, Phillip R. Zoladz

ONU Student Research Colloquium

Most individuals consume caffeine on a daily basis. Research has shown that caffeine stimulates the release of glucocorticoids and catecholamines and can enhance cognition. Caffeine also amplifies stress-induced increases in arousal and aggravates PTSD-like symptoms. Thus, we examined the impact of caffeine on memory for a stressful experience and intrusive memories related to that experience. Undergraduate students ingested caffeine or a placebo. Thirty minutes later, they were exposed to the Trier Social Stress Test (TSST; stress condition) or friendly-TSST (f-TSST; control condition), both of which were designed to enable participant memory for the experiences to be quantified. In each condition, …

Role Of Cb1 Receptors In Mediating Thermal Pain In Male And Female Mice, Jessica Acheampong, Sarah Seeley, Manoranjan S. Dsouza

ONU Student Research Colloquium

Pain is responsible for significant morbidity and mortality in the United States and all over the world. CB1 cannabinoid receptors have been reported to play a role in pain. Also, more recently sex-dependent behavioral differences after CB1 receptor activation have been reported. However, the sex-dependent effects of CB1 receptor activation on thermal pain have not been systematically assessed. This project will address this gap in knowledge. The study will use adult male and female C57BL6 mice. CB1 receptors will be activated using the synthetic cannabinoid WIN 55212. Thermal pain will be assessed using the hot plate method. Data for the …

Role Of Rpm-1 In A C. Elegans Apoe4 Alzheimer’S Disease Model, Lucille Mcdonald

Biomedical Engineering and Sciences Student Publications

Alzheimer’s Disease (AD) is characterized by the aggregation of β-amyloid plaques. The ε4 allele of APOE (APOE4) is linked to increased plaque formation in AD. Therefore, identifying genetic modifiers of APOE4 could present novel drug targets for AD treatment.

Assessing Developmental Neurotoxicity Due To Pyrethroid Insecticide Exposure In C. Elegans, Katharine Boyd, Timothy A. Crombie

Biomedical Engineering and Sciences Student Publications

Pyrethroids are a type of insecticide widely used in agriculture and public health programs. These insecticides disrupt neuronal signaling in target insects through voltage-gated sodium channels and have been linked to cardiovascular and neurological disease in humans.The nervous system of the nematode Caenorhabditis elegans is fully mapped, and specific behavioral circuits can be tested, making it a powerful model for studying neurotoxicity. Because the primary target of pyrethroids is not conserved in C. elegans, secondary mechanisms of pyrethroid neurotoxicity can be identified by testing whether specific behavioral circuits are affected.

Program And Proceedings, The Nebraska Academy Of Sciences 1880–2026: 146th Anniversary Year, One Hundred-Thirty-Sixth Annual Meeting

Nebraska Academy of Sciences: Programs and Proceedings

Program and abstracts of the proceedings for the Nebraska Academy of Sciences 136th annual meeting, 2025

Sessions

Aeronautics and Space Science

Anthropology

Biological and Medical Sciences

Earth Sciences

Ecology, Sustainability, and Environmental Science

Physics and Engineering

General Poster Session

2026 Maiben Lecture: Shifting Extremes: Understanding Nebraska’s Changing Climate and Preparing for What Lies Ahead, Deborah Bathke

2026 Friends of Science Awards: Julie Shaffer and Irina Filina

2026 C. Bertrand and Marian Othmer Schultz Colleaguate Schoalrship Award: Piper Ryschon and Bryce Reeson

In Memoriam: Paul Royster

Evaluating Drug Use Through Examination Of Local Water Sources: Drug Concentrations Within Biota Systems, Madeleine Freyhof

Honors Projects

Understanding drug usage based on drug concentrations found within biota samples grown in different concentrations of cocaine and benzoylecgonine. Drug policy then can be potentially based on how drug concentrations display drug usage for a specific community.

Ethnicity, Culture, And The Epidemiology Of Eating Disorders, Rebecca Atwell

Honors Theses

This systematic literature review addresses the research question: How does culture affect eating disorders? It examines the role of ethnicity and culture in shaping the prevalence, symptom presentation, and treatment of eating disorders across diverse populations. Although eating disorders have traditionally been studied in Western, predominantly White populations, growing evidence suggests that cultural norms, ethnic identity, globalization, and exposure to Western ideals significantly influence both the development and expression of disordered eating behaviors. Twenty peer-reviewed studies published between 2000 and 2024 were included. These studies were identified through systematic searches of PubMed, Scopus, Web of Science, and PsycINFO and selected …

Cultivating Curiosity Across Academic Audiences: Biology And Evidence-Based Science Communication, Nathan D. Forbes

Whittier Scholars Program

Effective Science Communicators must speak to a variety of audiences, with varying quantities of prior interest and qualities of background knowledge. The purpose of this paper was to engage differing audiences in scientific learning. This was done through a hypothetical newsletter made up for four different segments exploring a variety of topics in biology. Each segment intends to speak to a different audience with a different level of interest and/or educational level. Simplifying or analogizing without sacrificing the statistical basis of this project was of paramount importance. In the two segments targeted towards younger audiences, the primary focus was on …

Development Of A Preclinical Model That Can Be Used To Test Novel Therapeutics For The Treatment Of Mild And Moderate Traumatic Brain Injury, Hayden Anderson

Honors Theses

Traumatic Brain Injury (TBI) occurs when a foreign object induces damage to the skull and brain through a forceful blow or whiplash. It is one of the most frequent injuries in the United States, with approximately 250,000 cases of different levels occurring annually. It is well known that severe TBIs can result in persistent physical, cognitive, and socioeconomic consequences and the timing of treatment following this injury can play a critical role in the ultimate outcome. It would be beneficial to develop a safe therapeutic that could be administered rapidly after impact and improve the recovery of the patient. However, …

The Pam-1 Aminopeptidase Does Not Protect Against Ptl-1 Mediated Neurodegeneration In C. Elegans., Ethan E. Schuff

Honors College Theses

Debilitating neurodegenerative conditions are increasingly prevalent in our aging population, yet treatment options remain limited. Though neurodegenerative diseases vary in pathophysiology and clinical manifestations, these diseases are related to one another in that many involve misfolded proteins. Tau is a member of the microtubule-stabilizing MAPT/MAP2/MAP4 family, and misfolded tau proteins form the neurotoxic aggregates called neurofibrillary tangles (NFTs) found in the brains of patients with Alzheimer's disease and other neurodegenerative diseases. Designing a study to elucidate the neurotoxic role of tau is difficult because other MAPs may have compensatory mechanisms which confound study data. Caenorhabditis elegans (C. elegans) …

Assessment Of Goose-Beaked Whale Responsesto Mid-Frequency Active Sonar Usinga Hierarchical Hidden Markov Model, Stacy L. De Ruiter, Erin A. Falcone, Shannon N. Coates, Brenda K. Rone, David A. Sweeney, Karin A. Dolan, Susan M. Jarvis, Russel D. Andrews, Stephanie L. Watwood, Gregory S. Schorr

University Faculty Publications and Creative Works

Background

Acoustic disturbance is increasingly recognized as an ecological concern, particularly at sea. In light of marine mammal stranding events associated with use of naval mid-frequency active sonar (MFAS), which often involve goose-beaked whales (Ziphius cavirostris), research has sought to detect and characterize Ziphius behavioral responses to MFAS.

Methods

We deployed Sound and Motion Recording and Telemetry (SMRT) tags on 13 Ziphius in the Southern California Bight. The study area includes the United States Navy’s Southern California Anti-submarine Warfare Range (SOAR), and deployments overlapped temporally with training exercises including MFAS use. We used hierarchical hidden Markov models (HHMMs), which provided …

Phenotype Variations Among Chlamydomonas Reinhardtii Under Quorum-Sensing Conditions, Marcel Pedrosa

Biomedical Engineering and Sciences Student Publications

Quorum sensing (QS) is a cell-to-cell communication system that allows unicellular microorganisms like the eukaryote C. reinhardtii to regulate phenotypic switching as a function of cell density due to the accumulation of signaling molecules.  The goal of this study is to evaluate whether QS in C. reinhardtii is regulated by nutrient availability. We propose that in a nutrient-limited environment (low nitrogen TAP (N-TAP)), cells of C. reinhardtii (cc124) would adjust their motility-driven dispersion of cells and upregulate chlorophyll and carotenoid production per cell.

Anterior Cruciate Ligament (Acl) Injuries In Female Athletes: Implications For Prevention And Rehabilitation, Natalie Amm

Honors Theses

Anterior cruciate ligament (ACL) injuries are among the most common lower limb injuries and pose significant physical, psychological, and long-term functional challenges for athletes. Additionally, research has suggested that females are up to eight times more likely to experience an ACL injury than their male counterparts. This increased risk is multifactorial with risks including anatomical structure, neuromuscular control, strength deficits, and hormonal fluctuations. However, the elevated risk of ACL injuries in female athletes may not solely be caused by biological differences, but also due to training programs and sports being designed around male athletes. This pushes us to change the …

Investigating The Synergistic Effects Of Mental And Physical Exhaustion On Learning And Attention In Emergency First Responders: An Eye-Tracking Study, Kayla Page

Electronic Theses and Dissertations

ABSTRACT

Exhaustion is known to impair cognitive functioning and performance, yet its effects on learning during video-based instruction among first responders and military service members remain underexplored. This study examined how mental, physical, and combined exhaustion independently influence learning outcomes and visual attention in a physically active sample drawn from military, public safety, and emergency service roles. Visual attention was measured utilizing eye tracking, focusing on fixation duration (FD) and fixation rate (FR). Participants completed instructional tasks under each exhaustion condition, allowing comparison of learning and gaze behavior across fatigue states. Results indicated that mental and physical exhaustion were associated …

Multi-Strain Assessment Of Quorum Sensing In Chlamydomonas Reinhardtii, Jacqueline Krueger

Ocean Engineering and Marine Sciences Student Publications

Quorum sensing (QS) allows microbial populations to coordinate phenotypic switching, selecting for specific behaviors that are only beneficial when expressed at high cell densities (HCD, >106 cells/mL) rather than low cell densities (LCD, < 106 cells/mL)

Pepper Spray Exposure And The Effect On Dna Profiling Of Blood, Brandon Schmakel

Honors Projects

Pepper spray is a common self defense tool used in both law enforcement and civilian settings, with the goal of incapacitating the sprayed individual. In real casework where pepper spray was used, a red-brown stain which tested positive presumptively for blood provided no DNA profile after further testing six months later. A previous graduate student, Emma Crawn, attempted to emulate these casework conditions and instead achieved a DNA profile with slight indications of inhibition or degradation. Further analysis of active pepper spray ingredients such as Oleoresin Capsicum (OC) and Chloroacetophenone (CN) reveals that there are observed reactions, such as oxidation, …

How Pollinator Populations Can Impact Chronic Disease Risk, Kylee Cubbison

Honors Projects

This project investigates how pollinator populations can impact chronic disease risk. Pollinator populations are declining, which can have serious implications for human health. Declining pollinator populations can limit healthy food production and affordability. Poor nutrition is a major risk factor for most chronic diseases. Less healthy food available for people to consume can negatively impact diets and increase the risk of developing chronic diseases. This project aims to address a potential gap in research existing on this specific topic regarding pollinator populations and human chronic disease risk. A brochure targeted towards educating the general public on this topic was created …

From Bench To Bedside: Unraveling The Role Of Sarm1 In Chronic Human Neurodegenerative Disease, Caitlin Dingwall

Arts & Sciences Graduate Student Theses and Dissertations

Axon degeneration (AxD) is an early and often causative event in many neurodegenerative diseases, yet no treatments exist to halt the breakdown of axons. In healthy axons, the axon survival factor NMNAT2 inhibits SARM1, the central executioner of programmed axon degeneration. NMNAT2 is a highly labile protein produced in the soma and trafficked into the axon. Nerve injury blocks axonal transport and leads to rapid depletion of axonal NMNAT2, causing NMN buildup and NAD+ loss. Recent breakthroughs led to the discovery that SARM1 is activated by an increase in the NMN to NAD+ ratio. While the SARM1 axon degeneration pathway …

Development Of A Quantitative Top-Down Proteomics Platform For Arginylated Proteoform Analysis, Richard Myer Searfoss

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATION Development of a Quantitative Top-Down Proteomics Platform for Arginylated Proteoform Analysis by Richard Myer Searfoss Doctor of Philosophy in Biology and Biomedical Sciences Biochemistry, Biophysics, & Structural Biology Washington University in St. Louis, 2026 Professor Benjamin A. Garcia, Chair Top-down proteomics is a technique that utilizes mass spectrometry to identify and quantify biologically relevant proteoforms, or proteins with unique amino acid sequence and post-translational modification (PTM) signature. Challenges that exist in top-down proteomics include localization of PTMs, protein separation, and signal dilution. Here, we developed a top-down proteomics platform on a time-of-flight mass spectrometer that takes …

A Continuous Viral Vaccine Biomanufacturing Platform Utilizing Multiple Bioreactor Configurations, Justin Sargunas, Bradley Priem, Dylan Carman, Taravat Sarvari, Natalie Nold, Vaishali Sharma, Andrew Pekosz, Caryn Heldt, Michael Betenbaugh

Michigan Tech Publications

Scalable, continuous biomanufacturing processes have grown in importance to meet demand for smaller bioreactor sizes, lowered production costs, and improved quality attribute consistency. The Sf9/recombinant baculovirus (rBV) expression system demonstrates promise for virus-like particle (VLP) vaccine and gene therapy production. Here, we present a continuous rBV platform integrating an infection plug flow reactor (PFR) between stirred tank growth (gCSTR) and production (pCSTR) bioreactors. Cell expansion in the gCSTR included a ramp-up stage followed by continuous growth, reaching a steady state of 5×10 cells/mL and >90% viability. Péclet number-fit tracer studies confirmed near-ideal plug flow in the PFR, yielding a 10 …

High Performance Ae-Cnn Model Enhanced Via Gaussian Augmentation For Rssi-Dependent Indoor Positioning In Wireless Sensor Networks, Kahlaa K. Al-Nassrawy, Ghaidaa A Al-Sultany

Karbala International Journal of Modern Science

Precise indoor positioning system remains a significant challenge within Wireless Sensor Networks (WSNs) due to the instability and noise sensitivity of Received Signal Strength Indicator (RSSI) data. This paper proposes an advanced hybrid deep learning framework designed for 2D indoor localization, utilizing RSSI measurements to predict human or object position. Furthermore, to enhance the robustness of the model against signal variance and promote its generalization capability under this uncertainty, a data augmentation strategy is applied using three methods: Gaussian Noise Injection (GNI), Gaussian Mixture Model (GMM), and Bayesian Gaussian Mixture Model (BGMM). The purpose of data augmentation is to simulate …

Parasite Prevalence In Local Grasshopper Populations, Lucia B. Freund, Ali Gronquist

Research & Creative Achievement Day

Spur-throated grasshoppers (genus Melanoplus) are a common agricultural pest in North America. Spur-throated grasshoppers are also a common host to several parasitic organisms. Horsehair worms (phylum Nematomorpha) are parasitic organisms known for their ability to manipulate the behavior of its insect host, particularly grasshoppers and crickets, by urging the host to enter water so it can complete its life cycle. Along with being a host to parasitic worms, they are a frequent target of grasshopper mites. Grasshopper mites (Eutrombidium locustarum) is a short-lived parasite that can negatively influence the reproductive ability of their host. Because grasshoppers are a major agricultural …

Characterization Of Bacteria In Cattle Specimens, Jacob D. Hanson, Josie M. Tieskoetter

Research & Creative Achievement Day

Cattle nasal cavities provide a favorable environment for microbial colonization and can habit a wide population of bacteria, including opportunistic and pathogenic species. The objective of this research was to isolate, characterize, and identify bacteria present is nasal swap specimen collected from multiple calves. Samples were cultured and analyzed using selective and differential media, Gram staining, spore staining, and a range of biochemical assays. Several bacterial species were identified including the genus Corynebacterium and Streptococcus while some isolates could not be identified using classical biochemical methods alone. These results demonstrate that calf nasal specimens commonly contain diverse bacterial populations, some …

Establishment Of A Hek293t Cell Model System To Investigate Direct Intracellular Interactions Between Amyloid-Β (Aβ) And The Nlrp3 Inflammasome, Cristina Sinobas Pereira

Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and the leading cause of dementia worldwide. While Amyloid-β (Aβ) aggregation is a defining pathological feature, increasing evidence highlights chronic neuroinflammation as a critical contributor to disease progression. Soluble Aβ assemblies can act as damage associated molecular patterns (DAMPs) including activation of the NLRP3 inflammasome, which links amyloid pathology to sustained inflammatory responses in the brain (Heneka et al., 2015; Nakanishi et al., 2018). However, the molecular mechanisms by which specific Aβ conformations influence inflammasome assembly remain incompletely understood.This study aimed to investigate whether distinct aggregation states of amyloid β42 (Aβ42) are …

Analyzing 24-Hour Diet Recalls To Assess Dietary Changes Among Children Who Complete Oral Food Challenges (Ofcs), Hannah Plank, Wan Shen, Heesoon Lee

Honors Projects

Pediatric food allergies are a growing public health concern in America due to their potential association with long-term nutritional impacts and reduced quality of life in those affected. Oral food challenges (OFCs) are considered the gold standard for clinical food allergy diagnosis, but few studies have examined if passing or failing an OFC affects overall diet quality in infants. This clinical observational study examined differences in diet quality, measured by the Healthy Eating Index (HEI), among 39 children aged 0–6 years who participated in OFCs at ProMedica Allergy and Immunology Clinic in Perrysburg, Ohio. Parents and caregivers reported daily dietary …

The Effects Of Oleuropein In Olive Leaf Extract Alone Or Combined With Calcitriol On The Viability And Migration Of Human Endometrial Cells In Vitro, Katelyn Devereaux

Electronic Theses and Dissertations 2020 - Present

Endometriosis is a chronic inflammatory disease affecting approximately 10% of reproductive-aged women worldwide and is commonly associated with pelvic pain, infertility, and reduced quality of life. Current management strategies, including hormonal therapies and surgical intervention, often provide only temporary relief and are associated with recurrence and adverse effects. These limitations highlight the need for alternative, non-hormonal therapeutic approaches that target underlying inflammatory and cellular mechanisms of disease progression.

This study evaluated the effects of oleuropein, contained within an olive leaf extract, and calcitriol, the active form of vitamin D, on cellular viability and migration in immortalized human endometriotic epithelial (12Z) …

Cognition In Pair-Living Owl Monkeys: The Role Of Partner Affiliation In The Willingness To Cooperate To Solve A Cognitive Task, Aaron J. Mencia

Electronic Theses and Dissertations 2020 - Present

Cooperative problem-solving tasks, including the loose-string paradigm, require individuals to coordinate their actions simultaneously to obtain a shared reward. These paradigms have been used extensively to assess social cognition, behavioral coordination, and partner preferences across taxa. Although the loose-string paradigm has been widely tested, it has not been applied to pair-bonded, monogamous primates such as owl monkeys (Aotus nancymaae), which exhibit affiliative behaviors including food sharing and bi-parental care. Investigating cooperation in this species may offer some clarification on whether more stable social bonds are associated with cooperative and cognitive task success as compared to those observed in …

Modulating Acute Stress Reactivity: Behavioral Interventions And Caffeine-Linked Stress Physiology In College Students, Kaitlyn Smith

Honors Theses

College students frequently rely on caffeine to manage academic demands, yet its physiological and psychological effects on acute stress remain poorly understood. Although caffeine is known to increase cardiovascular arousal and self‑reported anxiety, little research has compared different caffeine sources within the same individuals or examined whether brief behavioral interventions can buffer these effects. Self‑affirmation, a short writing exercise centered on personal values, has been shown to reduce stress reactivity, but its interaction with caffeine‑induced arousal has not been tested. This within‑subjects study examines how coffee, energy drinks, and a no‑caffeine control influence acute cardiovascular and self‑reported stress responses, and …

Three Bacterial Endophytes Enhanced Plant Growth And Yield And Reduced The Severity Of Phytophthora Capsici In Bell Pepper And Tomato Plants In The Field, Daniel Ambachew, Margaret T. Mmbaga Mmbaga, Richard Hall, Peter Eyegheleme, Mustapha Olawuni, Jamille Robinson, Emily Rotich

Agricultural and Environmental Sciences Faculty Research

Naturally abundant endophytes colonize plants internally without causing harm to their host plants. Endophytes are likely to occupy the same ecological niches as phytopathogens and thus have a high potential to be effective biological control agents. Their demonstrated ability to suppress more than one plant pathogen suggests that they can offer a viable alternative to chemical fungicides and a strategy for decreasing the inoculum potential of soil-borne pathogens. Some biocontrol endophytes are also known to improve soil health and the overall health of plants. However, the results in greenhouse studies do not always translate to consistent field efficacy. In this …

Attempts To Increase Ear Height And Reduce Tillering In Sweet Corn, Charlie Rohwer, Jesen Hosch

Midwest Vegetable Trial Reports

Sweet corn is a major crop in Minnesota. Fresh-market growers supply seasonal produce, and the processing industry grows >80,000 acres. The hybrids grown for each use are different, but both fresh-market and processing growers are interested in ear height; too low, and mechanical or manual harvest is more difficult. Tillering is another trait of interest to growers, as it is thought that tillers reduce yield or interfere with harvest. To address these issues, we studied practices that may reduce tillering or increase sweet corn ear height. Elevated population reduced tillering, and so did two applications of Proliant™ plant growth regulator. …