Life Sciences Commons^™

The Effects Of Resistance Training On Older Adults: Increasing Healthspan And Lowering Disability Rates, Michael M. Samuel

Honors Undergraduate Theses

This study analyzed the research on older adults and resistance training exercise and assessed effects on health span and lowering disability rates. A targeted search of the literature was employed using the following key words: sarcopenia, resistance training, hypertrophy, quality of life, disability, activities of daily living, strength training, falls, and functional strength. These were used to find articles that were relevant to the research. Articles were excluded if they focused on young athletes as they did not meet my research age group. Articles that focused on nutrition were also excluded as they pulled the focus away from the impact …

Monitoring Faunal Responses To Biodegradable Oyster Reef Restoration Materials With Camera Traps, Tara L. Blanchard

Honors Undergraduate Theses

Restoration of the oyster reefs has become increasingly crucial due to great population declines around the globe. Intertidal oyster reefs provide essential foraging and loafing grounds to many faunal species, including several threatened/endangered wading bird species. Biodegradable oyster reef restoration materials have been introduced to avoid potential plastic pollution from traditional materials. Studies have shown success regarding oyster recruitment rates to these materials. However, their impacts on fauna using restored oyster reefs are unknown. This project aims to evaluate oyster reef restoration using biodegradable materials to increase faunal diversity, abundance, and foraging behaviors. Camera traps were deployed to observe fauna …

Examining The Effects Of Artemisia Annua On Neuro2a Cells, Wesley Lim

Honors Undergraduate Theses

Artemisia annua L. is a well-known medicinal herb used in Traditional Chinese Medicine for many centuries and contains artemisinin, which exhibits antimalarial properties [1] and is a potential treatment for SARS-CoV-2 [2]. However, the neurological effect of A. annua and its compounds are poorly understood. We hypothesize that A. annua extracts and artemisinin will provide protection against oxidative stress in mouse neuronal Neuro2A cells. Superoxide dismutase 1 (SOD1) is an anti-oxidant enzyme that protects against oxidative damage [3]. Growth Arrest and DNA Damage-inducible 45 alpha (Gadd45a) is involved in DNA repair and is a common biomarker for DNA damage[4]. SOD1 …

Examining Wrack In Mosquito Lagoon To Analyze Biodiversity And Seagrass Viability, Nicole Jerrell

Honors Undergraduate Theses

Seagrass restoration of Halodule wrightii, has become crucial as seagrass coverage in the Indian River Lagoon (IRL) declined by 58% between 2011 and 2019. To understand the abundance of seagrass fragments available for natural recruitment and restoration, we tracked the abundance of viable fragments found in the wrack in Mosquito Lagoon. Wrack is plant material, including seagrass fragments, mangrove propagules, and detritus. Seagrass fragments were considered viable if the fragment had an apical meristem present. Replicate samples were collected from 5 locations every two weeks, starting in September 2022 and ending in September 2023, and the samples were processed …

Investigating The Role Of Glycemic Control In Cognitive Impairement Risk Among Elderly Individuals With Type 2 Diabetes: A Scoping Review, Julia Pessaia

Honors Undergraduate Theses

Type 2 diabetes mellitus (T2DM), presents a significant health burden globally, affecting millions of individuals, especially in the elderly population. While its association with cardiovascular diseases and cognitive impairments is well-documented, further research on the precise influence of glucose control on cognitive outcomes in elderly T2DM patients is necessary. This scoping review aims to address this gap by investigating the impact of HbA1c levels representing glycemic control on the risk of developing cognitive impairments in elderly patients with T2DM. A literature search was conducted on MEDLINE and eligible studies involved T2DM patients aged 60 or older, with documented cognitive function …

Sweetening The Deal: Enhancing Flower Production And Quality For Native Pollinators In Urban Landscapes, Yaelle Esther Reeve

Honors Undergraduate Theses

Urban landscapes can support pollinator conservation, but traditional landscape designs lack the diverse mixtures of native plants needed to support native pollinators. Although native plants support pollinators and can survive with less water and fertilizer than non-native landscape plants, they do not always grow well under modified urban soil conditions and irrigation regimes. A better understanding of the factors influencing flower production and the quality of native plants is needed if we are to design landscapes that support pollinator conservation. This study focused on how flower production and quality in different native plants responded to compost and irrigation treatments. The …

Tim/Tam Receptors: A Potential Biomarker For Predicting Sensitivity To Zika Virus-Induced Oncolysis In Non-Small Cell Lung Cancers, Shankari Somasekar

Honors Undergraduate Theses

Non-small cell lung cancers (NSCLC) constitute 80-85% of lung cancers and are the leading cause of cancer-related deaths globally. The most common cause is prolonged smoking. Current treatment options for NSCLC include surgery, radiation, chemotherapy, targeted drug therapy, and immunotherapy. Although these medications are effective in the short term, patients often face issues of drug resistance and debilitating side effects with prolonged use. Currently, the use of Zika virus (ZIKV) is being researched as a possible alternative treatment for cancer, which minimizes side effects and the risk of drug resistance. TIM/TAM proteins are identified as the putative ZIKV receptors on …

A Review Of Dielectric Barrier Discharge Cold Atmospheric Plasma For Surface Sterilization And Decontamination, Kolawole Adesina, Ta Chun Lin, Yue-Wern Huang, Marek Locmelis, Daoru Frank Han

Biological Sciences Faculty Research & Creative Works

Numerous investigations have shown that non-equilibrium discharges at atmospheric pressure, also known as "cold atmospheric plasma" (CAP) are efficient to remove biological contaminants from surfaces of a variety of materials. Recently, CAP has quickly advanced as a technique for microbial cleaning, wound healing, and cancer therapy due to the chemical and biologically active radicals it produces, known collectively as reactive oxygen and nitrogen species (RONS). This article reviews studies pertaining to one of the atmospheric plasma sources known as Dielectric Barrier Discharge (DBD) which has been widely used to treat materials with microbes for sterilization, disinfection, and decontamination purposes. To …

Bioprinting With Adipose Stem Cells And Hydrogel Modified With Bioactive Glass, Krishna C.R. Kolan, Apurv Saxena, Bradley A. Bromet, Lesa B. Steen, August T. Bindbeutel, Julie A. Semon, Delbert E. Day, Ming C. Leu

Biological Sciences Faculty Research & Creative Works

Bioprinting research is focused on utilizing growth factors and multiple cell types to create clinically relevant three-dimensional (3D) tissue models using hydrogels. Rheological and biological challenges are two main factors that limit the creation of extrudable bioactive hydrogels. In this study, we investigate incorporation of fast dissolving and bioactive borate glass in different weight to volume percentages (0.075 to 0.6%) to alginate-gelatin (1:1) hydrogel to improve rheological properties and enable bioprinting with bioactive glass. The addition of glass improved the stiffness of the hydrogel. Human adipose-derived mesenchymal stem cells (ASCs) were uniformly mixed in this bioink at 1 x 106 …

Phytochemistry Of Isolated Essential Oils From Cautleya Spicata (Sm.) Baker For Their Pesticidal Evaluation And In-Silico Approaches To Reveal The Mode Of Nematicidal Action, Sonu Kumar Mahawer, Manar Fawzi Bani Mfarrej, Ravendra Kumar, Himani Karakoti, Om Prakash, Satya Kumar, Dharmendra Singh Rawat, Aditi Kundu, Pieter Malan, Faheem Ahmad

All Works

This study aimed to assess the chemical composition and pesticidal activities of essential oils (EOs) from Cautleya spicata rhizomes and aerial parts. The EOs were assessed against root-knot nematodes (Meloidogyne incognita) by mortality and hatchability bioassays and the phytotoxicity of the EOs was determined against Raphanus raphanistrum subspp. Sativus (radish) and Cichorium intybus (chicory) seeds. In-silico screening of the primary constituents of EOs and five chosen target proteins of the nematode was conducted to predict the expected mechanism of action of these oils. p-Cymene and β-pinene were the key compounds in the EOs from the rhizomes and aerial parts. The …

Acalypha Indica Aqueous Leaf Extract As Potential Nematicide Against The Root-Knot Nematode, Meloidogyne Incognita: In Vitro And Molecular Docking Studies, Altaf Khan, Manar Fawzi Bani Mfarrej, Fohad Mabood Husain, Qamar Zia, Mohd Adil, Mohammed Arshad, Khalid Umar, Niraj Kumar Jha, Pieter Malan, Soumya Pandit, Faheem Ahmad

All Works

Root-knot nematode (RKN) (Meloidogyne incognita) is a major plant parasitic nematode that severely damages crops, leading to significant yield losses and substantial economic impact globally. This study aims to investigate an environmentally sustainable biological strategy for mitigating parasitic populations of the root-knot nematode, M. incognita. Specifically, the research focuses on assessing the nematicidal efficacy of Acalypha indica against M. incognita mortality and second-stage juveniles’ (J2) hatching under controlled in vitro conditions. A. indica leaf aqueous extract was applied at varying concentrations (250, 500, 750, and 1000 ppm) to J2s and egg masses of M. incognita. Notably, at 1000 ppm, a …

Retrospective Awards Section, Sally Brown, Stephanie House-Niamke, Chansotheary Dang, Qazi Arka Rahman, Lara Farina, Colleen Moretz

Art in the Libraries Retrospective: 2015-2024

This section of the WVU Art in the Libraries Retrospective (2015-2024) includes a summary of awards given by the program to students, faculty and staff.

Characterizing Leaf Endophyte Composition In The Dune Grass Ammophila Breviligulata, Nabaa Majeed, Sarah Hobbs, Sarah Emery, Connor Morozumi, Natalie Christian

Undergraduate Research Events

Microorganisms living within plants play crucial roles in their health and function, yet these diverse communities that make up the plant microbiome remain poorly understood. The dune grass Ammophila breviligulata. is an ecosystem engineer which helps to build and stabilize dune ecosystems effectively acting as a buffer against storms to coastal communities. It is currently unknown whether the Ammophila microbiome plays a role in this plant species’ ability to transform dune ecosystems.. In this study, we assessed fungal diversity of endophytes—microbes living within plant tissues— in Ammophila leaves collected from Leelanau State Park, MI along the eastern shores of Lake …

The Efficiency Of Chlorophyll Fluorescence As A Selection Criterion For Salinity And Climate Aridity Tolerance In Barley Genotypes, Zied Hammami, Soumaya Tounsi-Hammami, Nhamo Nhamo, Saleh Rezgui, Yousef Trifa

All Works

Introduction: In the Near East and North Africa (NENA) region, crop production is being affected by various abiotic factors, including freshwater scarcity, climate, and soil salinity. As a result, farmers in this region are in search of salt-tolerant crops that can thrive in these harsh environments, using poor-quality groundwater. The main staple food crop for most of the countries in this region, Tunisia included, is barley. Methods: The present study was designed to investigate the sensitivity and tolerance of six distinct barley genotypes to aridity and salinity stresses in five different natural field environments by measuring their photosynthetic activity. Results …

Poor Water Quality As A Trigger Of Harmful Algal Blooms In Lake Manzala, Mohammed A. Hussein, Alaa Eldin Eissa, Reham M. Eltarabily, Mohamed F. Abdelghany, Mahmoud A. Elnakeeb, Eman M. Ismail, Reham H. Ragab, Amina A. Dessouki

Mansoura Veterinary Medical Journal

Objective: The current study aimed to assess the direct effects of poor water quality on the eruption of heavy harmful algal blooms and to assess the physicochemical properties of water in Lake Manzala, as well as the molecular identification of the retrieved green algae.

Procedures: Subsurface water samples were collected from three different sites in September 2023 for water quality assessment. Moreover, morphological and molecular identification of suspected harmful algae is the most reliable diagnostic tool for this eruption.

Results: This study was conducted in the northern and northeastern regions of Lake Manzala. These regions receive large amounts of municipal, …

Editing The Tri5 Gene Of Fusarium Graminearum Using Crispr/Cas9, Madelyn Rae Waters

Honors Program Theses

No abstract provided.

The Bird Family Tree: How All The Major Bird Groups Are Related To Each Other., Eric M. Ray

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This is a video project designed to help inform those who are curious about the shape of the bird family tree. This tree (or phylogeny) covers how the major groups of birds are related to each other. Though not a deep dive into the phylogeny (individual bird families are not covered, for example), this video seeks to give people the basic overview of how groups relate to each other. The total length is just under 30 minutes. At the start the video covers the basics on how to read a phylogeny, and then gets into the discussion of how the …

The Genetic Basis Of Two Reproductive Traits In Monkeyflowers: Stigma Closure And Corolla Carotenoids, Rachel Anne Halperin

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Abstract

The interactions between pollinators and flowers have long been a driving force for the evolution of many physical floral traits. Traits such as flower shape, size, color, and smell are just some of these traits that evolve because of these interactions. This evolution does not only occur in the more obvious morphological floral traits, however, but also in more subtle traits like touch sensitive stigma closure. In hundreds of Lamiales species, the bilobed stigma, the organ that receives pollen from pollinators, closes rapidly upon touch. Theory and experiments show that this novel dynamic reproductive trait increases pollen export and …

Carbon Allocation To Growth And Storage In Pinus Ponderosa During And Following Experimental Carbohydrate Limitation, Ella M. Keefer

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Understanding how plants allocate carbohydrates to growth and storage in the face of drought and other stresses that reduce carbohydrate availability is crucial to predict ecosystem responses to future stress events. Growth is critical for resource acquisition while storage of carbohydrates is critical as a safeguard during stress. Despite this, how plants allocate carbohydrates to growth and to non-structural carbohydrate (NSC) storage under drought and carbohydrate limitation is poorly understood. We examined how carbohydrate limitation, with and without drought, affects carbon allocation to growth versus stored NSCs. We found that under carbon limitation and depletion of stored NSC, NSC storage …

Interdisciplinary Learning: How Different Ways Of Thinking Can Fuel Passion And Expand Understanding., Allison Amy Peschek

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This Capstone project was created to show the profound experience resulting from an extended range of interdisciplinary learning, and how different methods, when paired together, can enhance a learning experience. This was a personal project of exploration into those different angles, and primarily focused on avenues of logical and creating learning, respectively. These methods have been implemented by this author often in the past, but this capstone will explore this concept further, as there is a lot of potential in combining these different methods and biases in learning, to both further passion and understanding as a learner, but to also …

Modifying A Health Education Program By Utilizing Methods From Community-Based Participatory Research Framework, Tashara Grandys

Graduate Research Theses & Dissertations

Purpose: This study determined the use of Community Based Participatory Research (CBPR) and guided the modification of the Whole Body Approach (WBA) health education program. The modified program was pilot tested and implemented among neighbors at Northern Illinois Food Bank. The impact of the modification was assessed via pre and post surveys. Background and significance: WBA is a 10-week program developed by faculty at Northern Illinois University and has been taught by graduate students in collaboration with the Northern Illinois Food Bank since 2016. The program is based on the Satter Eating Competence Model and Health at Every Size and …

Student Attitudes Towards Their Program Area Of Study In A College Of Health And Human Services, Melanie Regan

Graduate Research Theses & Dissertations

Poor retention rates can be an indicator of systematic problems within a university’s culture of academic affairs and administration. Improving student retention rates requires an understanding of why students are not returning to campus. This research aimed to develop and implement a survey analyzing students' sensory comfort, relevance, reaction to their area of study, sense of belonging, and self-efficacy within a College of Health and Human Sciences. A descriptive research design was utilized for this study to obtain information on the study population to survey participants' overall attitudes towards their experience in a program of study in a college of …

Workshop On Recreational Fisheries In Stock Assessments (Wkrfsa), International Council For The Exploration Of The Sea (Ices)

Fisheries Research Articles

ICES Technical Report Contributors (Editors)

Martina Scanu; Zachary Radford

Contributors (Authors)

David Curtis; Didzis Ustups; Eneko Bachiller; Estanis Mugerza; Gwladys Lambert; Hans Jakob Olesen; Ioannis Thasitis; Ivo Sics; Jonnathan White; Karina Ryan; Kieran Hyder; Kjell Nedreaas; Lennert van de Pol; Lucia Zarauz; Manos Koutrakis; Marie Storr-Paulsen; Martina Scanu; Matthew Farthing; Nicola Walker; Paul Bouch; Pia Schuchert; Sean Tracy; Simon Weltersbach; Sophy Phillips; Zachary Radford.

Executive summary

The Workshop on Recreational Fisheries in Stock Assessments (WKRFSA) aimed to establish a process for integrating recreational fisheries (RF) data into stock assessments. The workshop addressed three questions: identifying obstacles to RF inclusion, creating …

Weed Seed Wizard Scenario - Dormancy Shift In Barley Grass In Balaklava, South Australia, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity research reports

The Weed Seed Wizard is a national collaborative project that uses paddock management information to predict weed emergence and crop losses now and in the future.

The Weed Seed Wizard is a computer simulation tool that:

• applies to all Australian grain growing areas

• helps growers understand and manage weed seedbanks on their farms

• uses farm management records to simulate how different crop rotations, weed control techniques, irrigation, grazing and harvest management tactics can affect weed numbers, the weed seedbank and yields

• uses farm-specific management and site-specific weather

• is multi-species

See www.dpird.wa.gov.au for further information on Weed Seed Wizard.

This South …

Weed Seed Wizard Case Study - Grower From Western Australia’S Central Wheatbelt, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity research reports

The Weed Seed Wizard is a national collaborative project that uses paddock management information to predict weed emergence and crop losses now and in the future.

The Weed Seed Wizard is a computer simulation tool that:

• applies to all Australian grain growing areas

• helps growers understand and manage weed seedbanks on their farms

• uses farm management records to simulate how different crop rotations, weed control techniques, irrigation, grazing and harvest management tactics can affect weed numbers, the weed seedbank and yields

• uses farm-specific management and site-specific weather

• is multi-species

See www.dpird.wa.gov.au for further information on Weed Seed Wizard.

This case …

Weed Seed Wizard Scenario - Herbicide Resistance In Wild Oats In Wagga Wagga, New South Wales, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity research reports

The Weed Seed Wizard is a national collaborative project that uses paddock management information to predict weed emergence and crop losses now and in the future.

The Weed Seed Wizard is a computer simulation tool that:

• applies to all Australian grain growing areas

• helps growers understand and manage weed seedbanks on their farms

• uses farm management records to simulate how different crop rotations, weed control techniques, irrigation, grazing and harvest management tactics can affect weed numbers, the weed seedbank and yields

• uses farm-specific management and site-specific weather

• is multi-species

See www.dpird.wa.gov.au for further information on Weed Seed Wizard.

This New …

Agave Tequilana - Environmental Weed Risk Assessment 2024, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Agave tequilana. Agave tequilana, commonly called blue agave or tequila agave, is a succulent native to the states of Jalisco, Colima, Nayarit and Aguascalientes in Mexico. In its native range lue tequila predominantly grows at high altitudes ( > 1,500m) on iron-rich, fertile soils of volcanic origin (Chambers and Holtum 2010). The ‘Jalisco’ region where A. …

Excited State Proton Transfer From Acidic Alcohols To A Quinoline Photobase Can Be Solvated By Non-Acidic Alcohol Solvents, Jonathan Ryan Hunt, Joseph Hecht, Clara Goolsby, Jade Hagihara, Monique Loza, Samantha Del Pozo

Chemistry and Biochemistry Faculty Works

Photobases are a type of molecule that become more basic upon photoexcitation and can therefore be used to control proton transfer reactions with light. The solvation requirements for excited state proton transfer (ESPT) in photobase systems is poorly understood, which limits their applicability. Here, we investigate the solvation of the ESPT reaction using 5-methoxyquinoline (MeOQ), a well-studied photobase with an excited state pKa (pKa*) of approximately 15.1, as a model system. Previous studies have shown that, in addition to the acidic donor that donates a proton to the photoexcited MeOQ, an additional “auxiliary donor” is necessary to solvate the resulting …

A Cytosine Analogue 5-Azacitidine Improves The Accumulation Of Licochalcone A In Licorice Glycyrrhiza Inflata., Xiaoling Ma, Ningxin Jiang, Jingxian Fu, Yuping Li, Lijun Zhou, Ling Yuan, Ying Wang, Yongqing Li

Plant and Soil Sciences Faculty Publications

Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, …

The Mechanism Of Sperm (Pro)Nuclear Migration In Plants And Animals, Yilin Zhang, Tomokazu Kawashima

Plant and Soil Sciences Faculty Publications

Gamete nuclear migration is a critical process during fertilization in flowering plants, yet its molecular mechanisms remain poorly understood. Recent studies have highlighted the essential role of cytoskeletal elements, particularly F-actin, in directing sperm nuclear migration, contrasting with the microtubule-driven migration in animals. We summarize the process of sperm nuclear migration in plants and the involvement of Class XI myosin XI-G in Arabidopsis, along with the ROP8-SCAR2 pathway’s ARP2/3-independent mechanism for F-actin nucleation. We also provide a comparative overview of examples from sea urchins, C. elegans, mice and yeast contrasting these mechanisms with those in plants. Finally, we …