Life Sciences Commons^™

Understanding The Role Of Early-Stage Seed Development On Final Seed Weight By Linking Seed Physiology, Transcriptomics, And Environments In Soybean (Glycine Max (L.) Merr.), Ashwini Shivakumar

Theses and Dissertations--Plant and Soil Sciences

Soybeans, which are widely grown in the United States, are used as food, animal feed, and industrial oil. The rise in plant-based diets highlights the high protein content and versatility of soybean. Understanding seed development is crucial for increasing yield. Soybean seed development includes three phases: lag, seed-filling, and maturation. Post-fertilization, the seed undergoes cell division in the lag phase, forming the embryo, endosperm, and seed coat. The embryo absorbs the endosperm and develops into two cotyledons. During the seed-filling phase, cotyledon cells accumulate starch, proteins, and lipids. During maturation, seeds dry out and become quiescent. Studies indicate that the …

Nano-Pulse Treatment Overcomes The Immunosuppressive Tumor Microenvironment To Elicit In Situ Vaccination Protection Against Breast Cancer, Anthony Nanajian, Megan Scott, Niculina I. Burcus, Brittney L. Ruedlinger, Edwin A. Oshin, Stephen J. Beebe, Siqi Guo

Bioelectrics Publications

We previously reported that nano-pulse treatment (NPT), a pulsed power technology, resulted in 4T1-luc mammary tumor elimination and a strong in situ vaccination, thereby completely protecting tumor-free animals against a second live tumor challenge. The mechanism whereby NPT mounts effective antitumor immune responses in the 4T1 breast cancer predominantly immunosuppressive tumor microenvironment (TME) remains unanswered. In this study, orthotopic 4T1 mouse breast tumors were treated with NPT (100 ns, 50 kV/cm, 1000 pulses, 3 Hz). Blood, spleen, draining lymph nodes, and tumors were harvested at 4-h, 8-h, 1-day, 3-day, 7-day, and 3-month post-treatment intervals for the analysis of frequencies, death, …

Animal Personality And Sentience As Distinct Concepts, Mark Briffa

Animal Sentience

Owens et al (2024) discuss how knowledge of both animal personality and sentience in target populations can be leveraged to enhance wildlife conservation programmes. In this commentary I expand on the distinction between these two concepts. Behavioural differences should be considered broadly across conservation programmes, not just those involving species deemed sentient.

Relationship Between Socking Rate And Production Of Stocker Hybrid Tilapia And Water Quality In A Mixtotrophic Biofloc System, Bartholomew W. Green, Steven D. Rawles, Candis L. Ray, Matthew E. Mcentire

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Intensified production of stocker-size tilapia can be accomplished using the biofloc technology production system. Stocker-size tilapia (ca. 10–200 g/fish) are raised for subsequent stocking for grow-out to market size. The relationship between stocking rate and the production of stocker-size hybrid tilapia (Oreochromis aureus x O. niloticus) was evaluated in two studies in outdoor mixotrophic biofloc tanks. Hybrid tilapia were stocked in 18.6-m² (15.7-m³) tanks at 50–150 fish/m² (59–178 fish/m³) in 25 fish/m² (30 fish/m³) increments in Study 1 and grown for 141–143 days. Stocking rates evaluated in 4.7-m …

Borate-Based Bioactive Glasses Properties: Clinical And Biomedical Applications, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Borate bioactive glasses (BBGs) constitute the recently produced bioactive glasses (BGs) in the domain of bioactive materials, offering enhanced functionalities over conventional silicate and phosphate BGs. This advancement is primarily characterized by BBGs' rapid yet controllable degradation rates, superior bioactivity, and the ability to swiftly form a hydroxyapatite layer (HAP) upon interaction with physiological fluids. A distinguishing feature of BBGs lies in their customizability; doping with therapeutic metal ions tailors their bioactivity towards specific physiological responses, including promoting angiogenesis, antibacterial activity, and osteogenesis. This versatility of BBGs' applications extends to bone regeneration, wound healing, drug delivery systems, and potentially nerve …

Investigating Ammonium Toxicity In The Performance Of Recirculating Periphytic Algal Wastewater Treatment Systems, Oluchuku Richie Ochonogor

Theses and Dissertations

Freshwater nutrient enrichment from wastewater facilities and other sources can lead to freshwater eutrophication, a threat to global aquatic ecosystems. Mechanical and chemical ways to curb this threat are either too expensive or not sustainable, and thus, not feasible. Compared to mechanical and other methods, sustainable, inexpensive biological methods (for example, algal treatment systems) have therefore been developed for the removal of excess nutrients from wastewater. By design, secondary waste treatment facilities (WWTF) remove organics and solids and lower oxygen demanding substances; however, they may not remove enough nutrients to protect freshwater ecosystems in all cases. While algae-based biological methods …

A Phytochemical Analysis Of Secondary Metabolites In Eupatorium Serotinum, Elise Snow

Theses and Dissertations

Phytochemistry, a subset of Natural Products Chemistry, studies chemically derived products from natural substances. Advances in this field have important applications in medicinal research due to the distinctive and unique properties of natural compounds. All metabolites are produced from the metabolic cycle, which is a pathway that provides energy for the organism. These metabolic compounds vary greatly and are classified based on their function within the organism. Primary metabolites are abundant and contribute to organism growth, development, and maintenance of energy. Secondary metabolites are found in lesser quantity and correlate to natural defense mechanisms. For example, salicylic acid is a …

Stable Isotope Analysis Of Manatee Vibrissae To Investigate Individual Patterns Of Resource Use In The Northern Gulf Of Mexico, Levette Tucker, Mackenzie L. Russell, Elizabeth E. Hieb, Ruth H. Carmichael, Carl S. Cloyed

Gulf and Caribbean Research

No abstract provided.

Advancing The Blue Economy Through Sustainable Mariculture: The Prospect Of Pearl Oyster And Sponge Cultivation In St. Vincent And The Grenadines, Kevin Antonio Providence

Gulf and Caribbean Research

The Blue Economy is estimated to be worth over USD 2.5 trillion annually, or almost 3% of the world’s GDP in 2020. St. Vincent and the Grenadines (SVG) in the Caribbean region has a vast and rich marine space that is underutilized. This study aimed to explore the feasibility of pearl oyster and sponge farming to advance the country’s Blue Economy through sustainable mariculture. A qualitative approach was applied, using a literature review and thematic analysis of data gathered through semi—structured interviews and focus group discussions with relevant stakeholders. Among several species evaluated, the result of the study revealed that …

Stable Isotope Analysis Of Antipatharian Diets From The Northwestern Gulf Of Mexico, Keir J. Macartney, Victoria Salinas, Zachary Rivera, David Hicks

Gulf and Caribbean Research

Antipatharians, or black corals, form a key component of the benthic fauna found throughout the Gulf of Mexico (GOM). In the northwestern GOM, they are a common member of the communities found on the natural relic coral—algal banks known as the South Texas Banks as well as on artificial reefs. They are known to provide important habitat for many ecologically and economically important species in the area but are relatively understudied. Here we present a study on their trophic ecology using stable isotope analysis of carbon and nitrogen from 2 species, Antipathes atlantica and Stichopathes luetkeni from a mesophotic artificial …

Development Of Fungal Hydrogels For 3d Bioprinting And Cellular Agriculture, Lauren E. Doyle

Theses and Dissertations--Biosystems and Agricultural Engineering

Hydrogel scaffolds are an important component to cellular agriculture. There is a need for a sustainable alternative to the commonly used gelatin-sodium-alginate hydrogels. Specific edible fungus species may provide a suitable alternative. Many fungi possess high protein contents, ease of cultivation, and strong functional properties. However, functional characterizations and application of fungal flour and protein extracts in cellular agriculture are lacking in the literature. Therefore, this study’s objectives were to 1) Develop processes to create functional fungal protein fractions from different fungal strains and evaluate their characteristics, and 2) Quantify the printability of the resulting fungal gels for the creation …

Pulse-Width Modulation Spray System Performance And Development Of A Novel System To Optimize Spray Use Efficacy, Prashanta Pokharel

Theses and Dissertations--Biosystems and Agricultural Engineering

The overarching objective of this research was to enhance our understanding of pulse width modulation (PWM) based nozzle control system and develop an intelligent nozzle control system for agricultural spray applications. This dissertation focused on measuring and analyzing the transient nozzle pressure characteristics and performance under PWM control. Additionally, it explored the development of an innovative multi-degree-of-freedom (DOF) nozzle control system for precise chemical applications in specialty crop systems.

The pressure dynamics of a PWM system were studied by developing an instrumentation system capable of operating a solenoid valve and recording pressure signals. A nozzle body and two manifolds of …

Batch Culture Models Of The Murine Gut Microbiome & The Impact Of Simple Dormancy On Dormancy-Capable Microorganisms Models, Ana C. Mendez

Mathematics Dissertations - Archive

The proposed mathematical biology research utilizes mathematical models to gain insight into biological systems. These systems of ordinary differential equations model diverse topics, ranging from gut microbiomes to harmful algal blooms. A complete stability analysis, supporting phase plane portraits, bifurcation diagrams, and numerical simulations will accompany the models presented. In Chapter 2, the murine gut microbiome is modeled to match laboratory experiments in the literature. In these experiments, mice eat plasmid-carrying “donor” bacteria and naturally carry plasmid-free “resident” bacteria in their gut. The models aim to capture the behavior of plasmids, donor bacteria, and resident bacteria. Chapter 3 explores dormancy …

Biology News 2024, University Of Northern Iowa. Department Of Biology.

Biology News

Contents:

Letter from the Department Head --- 2
Bolton Award Winner --- 3
Goldwater Scholarship Winner --- 3
Purple and Old Gold Award Winner --- 3
Faculty News Roundup --- 4
New Hires --- 6
UNI Botanical Center --- 7
Alumni Information Request --- 7
Support Our Programs --- 8
Floyd Fellowship Award Winner --- 8

A Survey Of Strongyle Nematodes In New England Dairy Goats, D.J. Richardson, E.M. Wray, D. Watters, U. Hanif, E. Jalbert, E. Thompson, G. Trajkovic, A.R. Sirois, N. Pilotte

Journal of the Arkansas Academy of Science

The paucity of information concerning the occurrence of strongyle nematodes in New England dairy goats prompted a survey of 241 dairy goats from throughout New England. Of the 241 goats examined using the McMaster technique, 104 (42.7%) were infected with strongyles with a mean intensity (+ SE) of 369.7 + 45.8 eggs per gram. Subsequent molecular examination of 50 random samples from these goats revealed a prevalence of 88% infection specifically with Haemonchus contortus. Haemonchus contortus is the most important internal parasite of sheep and goats, exerting a crushing economic burden on the livestock industry worldwide due to lowered milk …

Bat Versus Ant: The First Report Of Evening Bat/Ant Interaction In Arkansas, J.D. Wilhide, D.B. Sasse, R.P. Kelso

Journal of the Arkansas Academy of Science

This paper documents the first reported occurrence of bat/ant agonistic interaction in Arkansas and the second report involving Evening Bats (Nycticeius humeralis). An adult male Evening Bat was captured during a presence / absence mist net survey along Highway 412 in Black Rock, Lawrence County, Arkansas on 4 June 2023. The bat weighed 12.0 g and had a forearm length of 36.94 mm which is in the midrange for evening bats in Arkansas (Sealander and Heidt 1990). The evening bat had two ant head capsules attached to its right cheek and at the tip of the lower lip (Fig. 1).

Driving Cars Down Memory Lane: Uncovering Car Memory T Cell And Antigenspecific Endogenous T Cells Importance In Durable Responses Against Melanoma, Asmaa Mohamed

Dartmouth College Ph.D Dissertations

Responsiveness to chimeric antigen receptor (CAR) T cell therapy correlates with CAR T cell expansion and persistence in vivo. Multiple working strategies improve CAR T cell persistence by increasing stem-like properties or by sustaining CAR T cell activity with combination therapies. Herein, we describe the intrinsic ability of CAR T cells to differentiate into memory T cells, the effect of cytokine armoring, and neoadjuvant CD4 depletion therapy on CAR and tumor-specific endogenous memory T cells. TRP1-specific CAR T cells alone or in combination with Super2 + IL-33 (S233) armoring and/or CD4 depletion therapy were evaluated in the immunocompetent B16F10 melanoma …

Weed Seed Wizard Scenario - Glyphosate Resistance In Barnyard Grass In Goondiwindi, Queensland, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity research reports

The Weed Seed Wizard is a national collaborative project that uses paddock management information to predict weed emergence and crop losses now and in the future.

The Weed Seed Wizard is a computer simulation tool that:

• applies to all Australian grain growing areas

• helps growers understand and manage weed seedbanks on their farms

• uses farm management records to simulate how different crop rotations, weed control techniques, irrigation, grazing and harvest management tactics can affect weed numbers, the weed seedbank and yields

• uses farm-specific management and site-specific weather

• is multi-species

See www.dpird.wa.gov.au for further information on Weed Seed Wizard.

This Queensland …

Sclerotinia Management For Narrowleaf Lupin Crops In Western Australian Farming Systems Daw2104-002rtx: Project Results Summary For 2021-2023 Research Results, Ciara Beard, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity research reports

Extensive research has been conducted in 2021-2023 to expand our understanding of the distribution of Sclerotinia sclerotiorum incidence in lupin in WA, the infection process, yield and grain quality impacts, and management strategies. The research was conducted via commercial crop surveys, and experiments in the field, lab, controlled environment and glass houses by Department of Primary Industries and Regional Development (DPIRD) and collaborators Centre for Crop and Disease Management (CCDM), Mingenew Irwin Group (MIG), and lupin growers.

Wa Insecticide Guide 2024 - Winter Spring, Svetlana Micic, Alan Lord

PestFacts WA

The insecticides listed in the tables within this guide can be used on any crop appearing on the chemical label, if the rate used does not exceed the highest rate that is registered for use on that crop.

There are many products with different trade names that contain the same active ingredient. This list is not exhaustive and does not imply any specific recommendations of brand names.

Inflammation And Tumor Progression: The Differential Impact Of Saa In Breast Cancer Models, Daniel Wilhelm Olivier, Carla Eksteen, Manisha Du Plessis, Louis De Jager, Lize Engelbrecht, Nathaniel Wade Mcgregor, Preetha Shridas, Frederick C. De Beer, Willem J. S. De Villiers, Etheresia Pretorius, Anna-Mart Engelbrecht

Saha Cardiovascular Research Center Faculty Publications

Background: Previous research has shown that the Serum Amyloid A (SAA) protein family is intricately involved in inflammatory signaling and various disease pathologies. We have previously demonstrated that SAA is associated with increased colitis disease severity and the promotion of tumorigenesis. However, the specific role of SAA proteins in breast cancer pathology remains unclear. Therefore, we investigated the role of systemic SAA1 and SAA2 (SAA1/2) in a triple-negative breast cancer mouse model.

Methods: Syngeneic breast tumors were established in wild-type mice, and mice lacking the SAA1/2 (SAADKO). Subsequently, tumor volume was monitored, species survival determined, the inflammatory profiles of mice …

The Effectiveness Of Constructed Treatment Wetland Systems At Removing Microplastic Pollution, Katherine L. Johnsen

Honors Undergraduate Theses

Plastics are a highly utilized material in modem society, leading to environmental concerns surrounding plastic pollution. Microplastics, which are defined as plastic particles less than 5mm in size, have additionally become increasingly recognized as a harmful pollutant in aquatic systems. Constructed treatment wetland systems (CTWSs) are highly effective at removal and mitigation of pollutants, including anthropogenic nutrient inputs. This study aims to understand the potential of a CTWS to remove microplastic pollution from tertiary-treated municipal wastewater before discharge into a local natural water body. Microplastic percent removal was determined through the manual quantification of microplastics in triplicate 500mL surface water …

Novel Engineering Approaches For Orthopedic Injury Treatment And Infection Diagnosis, Cynthia Co

Bioengineering Dissertations - Archive

Bioengineering has made significant contributions to the treatment of orthopedic injury through interdisciplinary approaches. Central to these advancements are many orthopedic implants, including anchors and joint replacements, which have been designed to restore mobility. Despite their functional efficacy, these products have limited lifespan and are associated with many complications, such as implant-associated infection. The success of implant design and function, both in the short and long term, is paramount for enhancing patient outcomes and overall well-being. Therefore, my thesis work is dedicated to addressing these critical challenges, aiming to maximize their treatment effect while minimizing the implant-associated complications.

Long-term patient …

Transient Interactions Modulate The Affinity Of Nf-Κb Transcription Factors For Dna, Tianjie Li, Shandy Shahabi, Tapan Biswas, Oleg V. Tsodikov, Wenfei Pan, De-Bin Huang, Vivien Ya-Fan Wang, Yi Wang, Gourisankar Ghosh

Biology Faculty Publications

The dimeric nuclear factor kappa B (NF- κB) transcription factors (TFs) regulate gene expression by binding to a variety of κB DNA elements with conserved G:C-rich flanking sequences enclosing a degenerate central region. Toward defining mechanistic principles of affinity regulated by degeneracy, we observed an unusual dependence of the affinity of RelA on the identity of the central base pair, which appears to be noncontacted in the complex crystal structures. The affinity of κB sites with A or T at the central position is ~10- fold higher than with G or C. The crystal structures of neither the complexes nor …

Digital Health Care: Time For A New Standard?, Z K. Winkelmann

Clinical Practice in Athletic Training

Introduction: The rapid influx of digitalization in healthcare has presented opportunities for innovative engagement, delivery, and outcomes for patient care. However, it also presents with several barriers and challenges that require teaching and learning opportunities to prepare future athletic trainers for these tasks. Currently, the CAATE curricular content standards integrate digital health care through Standard 64 on health informatics and Standard 87 on biometrics. However, the art and science of digital healthcare are expansive, including various skills such as digital literacy, computer skills, online communication, and privacy and security standards. Methods: The presentation will provide an overview of current digital …

Burnout In Athletic Training: Measured By Survey In Most Common Settings For Athletic Trainers, R Johnson Krug, N Sand, S Brooks, T Smith

Clinical Practice in Athletic Training

Introduction: Burnout is a common problem in athletic training, affecting both athletic trainers (ATs) and the athletes they serve. Athletic trainers are responsible for the prevention, diagnosis, and treatment of sports-related injuries. Long work hours, often including evenings and weekends, as well as frequently under pressure to meet the demands of athletes, coaches, and parents. Athletic trainers face numerous stressors that contribute to burnout. These stressors may include heavy workloads, lack of control over their schedules, time pressures, and pressure to produce positive outcomes. Athletic trainers may also experience emotional and physical exhaustion, depersonalization, and a reduced sense of personal …

Educational Outcomes Of The Dat: A Document Review And Thematic Analysis, S H. Orr, L E. Eberman, M J. Rivera

Clinical Practice in Athletic Training

Context: The transition in athletic training education allows for unique and new opportunities in postprofessional education. The Doctor of Athletic Training (DAT) degree is one of the postprofessional education pathways that could provide athletic trainers advancement in clinical practice, practice leadership, and scholarship. However, there is no data on the educational outcomes of DAT programs and what graduates should expect from their experiences. The aim of this study was to explore whether DAT programs are delivering curricula consistent with the development of clinical scholars with advanced knowledge and skills that are needed for high-level patient care and literature relative to …

Athletic Trainers’ Familiarity With Potential Concussion Biomarkers: A Descriptive Study, T R. Campbell, B J. Davis, J C. Martinez, J M. Cavallario

Clinical Practice in Athletic Training

Introduction: An estimated 3.8 million individuals sustain a concussion annually in the United States. Despite the high number of cases, this number may not accurately depict the true incidence rate due to underreporting of symptoms by patients. With current concussion tools relying heavily on subjective input from patients, it is imperative that a valid and reliable objective tool, such as a clinical biomarker, is identified to improve concussion diagnosis and management. If such a biomarker is identified, it will be essential that athletic trainers (ATs) be familiar with how biomarkers could be used. The purpose of this study was to …

Athletic Training Preceptors’ Experience With Interprofessional Education And Collaborative Practice In The Clinical Learning Environment, A Bender

Clinical Practice in Athletic Training

Introduction: Professional athletic training (AT) programs are now required to align their educational curricula with the Institute of Medicine's (IOM) core competencies, preparing students to work in dynamic, interprofessional healthcare teams to strategically address patient outcomes. Although interprofessional education and collaborative practice (IPECP) is commonly implemented in didactic settings, our understanding of how IPECP is implemented by AT preceptors in various clinical settings is limited. The purpose of this study was to gain a greater understanding of AT preceptors’ experience with IPECP and how they implement this with students in their clinical settings. Methods: A convergent mixed-methods design was used …

Using Alums Involvement To Increase Student Investment In An Athletic Training Program, C J. Heffner, A L. Green

Clinical Practice in Athletic Training

Introduction: Student investment and retention are not new concerns among athletic training program directors. Graduate programs are expected to meet minimum enrollment standards to demonstrate the need for the program. Programs that are unable to maintain student enrollment will be eliminated by institutions experiencing financial stress. Retention strategies and benefits have previously been investigated among undergraduate students and across disciplines. One common theme in the research is that student retention increases when students feel a sense of belonging from the faculty, staff, and peers in their program. There is less research on the retention of graduate students, but the themes …