Life Sciences Commons^™

How Environmental Variation Affects Bumblebee Cognition And The Gut Microbiome, Parker Madison Campbell

Graduate Theses/Dissertations

This master's thesis examines the influence of environmental variables on cognitive processes in Bombus impatiens, focusing on the effects of ingested microbial communities on gut microbiota and their subsequent impact on associative color learning. The study implemented controlled feeding trials with isolated microbe species (Candida bombi, Metschnikowia reukaufii, Lactobacillus micheneri and a control with no microbes) to analyze shifts in gut microbial composition in foraging bees. Results indicated minor alterations in the microbial profiles of bees, yet no statistically significant correlation was found between these modifications and variations in learning or memory performance. Furthermore, the study assessed the impact of …

Regina Olsen Hughes Essay, Valencia Crosby

A with Honors Projects

An essay about scientific illustrator Regina Olsen Hughes and her experiences living with deafness and using sign language as a tool for success.

Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya

Selected Full-Text Master Theses 2021-

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key …

Characterizing Sex Differences In Hpv Negative Head And Neck Squamous Cell Carcinoma, Sarah Barclay

Dissertations and Theses

It is now widely accepted that peripheral solid tumors are infiltrated by nerves. This infiltration of malignancies by nerves is referred to as tumor innervation. The Vermeer laboratory studies tumor innervation primarily in head and neck squamous cell carcinoma using a syngeneic cell line representing a mutationally-induced form of the disease; these cells are called MOC2-7 cells. In addition to defining the molecular consequences of tumor-infiltrating nerves on disease, the lab also focuses on their influence on behavior. This is important as cancer patients have a significantly higher incidence of depression and anxiety as compared to the population at large. …

Deepwhalenet: A Climate Change-Aware Fft-Based Neural Network For Underwater Passive Acoustic Monitoring, Nicholas Ryan Rasmussen

Dissertations and Theses

In the face of escalating climate threats, the conservation of whale species has become increasingly critical. Traditional acoustic monitoring methods, burdened by extensive pre-processing and post-processing, need more adaptability and efficiency for effective marine mammal surveillance. This study introduces DeepWhaleNet, a novel deep-learning framework tailored for Underwater Passive Acoustic Monitoring (UPAM). DeepWhaleNet is designed to streamline whale detection by directly analyzing raw log-power spectrograms, thus extracting essential acoustic features to conserve these endangered species. The framework employs an extensive short-time Fourier transform (STFT) for input processing and a customized ResNet-18 architecture for classification, distinguishing whale vocalizations from ambient noise and …

Exploring The Role Of Nonmuscle Myosin Ii In Renal Thick Ascending Limb Epithelial Cell Protein Trafficking And Organelle Architecture, Brook Busselman

Dissertations and Theses

The traditional functional roles of the actin associated motor protein nonmuscle myosin II (NM2) have been thoroughly studied. However, the nontraditional roles for NM2 in the context of membrane remodeling and protein trafficking events in cells have just recently been unraveled. Our work has found that genetic inactivation of NM2 protein isoforms MYH9 and MYH10 in the renal epithelium of adult mice leads to progressive kidney disease. Aberrant protein transport and localization in cells of the thick ascending limb (TAL) of the kidney nephron is accompanied by upregulation of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) pathway. …

Regional And Landscape Scale Examination And Attribution Of Vegetation Changes To Social-Environmental System Drivers In Kazakhstan, Venkatesh Kolluru

Dissertations and Theses

Kazakhstan (KZ) experienced widespread changes in ecosystem structure and function. The country is a climate and land cover/use change “hotspot” owing to political reforms, intensified grazing, and extreme climatic events. Despite their importance, there is a lack of consensus about vegetation changes, trends, and drivers in KZ. Addressing this knowledge gap is crucial for effectively managing and restoring grassland ecosystems. However, a pressing challenge is discerning anthropogenic-driven vegetation changes from climate variability and decomposing the responses to the complex human-environmental forcings. Motivated by these challenges, I employed statistical and machine learning algorithms to detect and attribute vegetation changes to social-environmental …

Assessing The Utility Of Breast Cancer Polygenic Risk Scores And Association With Clinical Factors In A Population Of Breast Cancer Patients, John L. Slunecka

Dissertations and Theses

INTRODUCTION: Breast cancer (BC) is the most common cancer among women and is classified as a complex disease. Advances in population genomics have led to the development of polygenic risk scores (PRSs) with the potential to enhance current risk models, but replication is often limited. OBJECTIVE: We sought to assess the predictive capabilities of two high-powered BC PRSs in a sample population selected for breast cancer. In addition, the capacity of the PRSs to predict clinical variables that could improve BC screening and treatments was explored. METHODS: Two published PRS algorithms (313 vs 3820) were used to score female subjects …

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …

Forage News [2024-01], University Of Kentucky

Forage News

No abstract provided.

Forage News [2024-12], University Of Kentucky

Forage News

No abstract provided.

Diapause In Eastern North American Populations Of Coccinella Septempunctata (Coleoptera: Coccinellidae), John J. Obrycki

Lady Beetle Research Data

Experiments examined the effect of photoperiod on the expression of adult diapause in the Palearctic ladybird beetle Coccinella septempunctata, within 10 years of its discovery in North America. The study used beetles from two populations in eastern United States and focused on two aspects of diapause. First, the effects of daylength on the induction and depth (or duration) of diapause were examined by rearing and maintaining adult beetles from Tompkins County, New York,(approx. 42.4°N, 76.5°W) under six constant photoperiods until oviposition, death, or the passage of 125 days. Second, the seasonal progression of diapause maintenance and termination under natural conditions …

Diapause Induction In Eastern North American Populations Of Propylea Quatuordecimpunctata And Hippodamia Variegata (Coleoptera: Coccinellidae), John J. Obrycki

Lady Beetle Research Data

Experiments examined the effect of photoperiod on the expression of adult diapause in North American populations of two Palearctic ladybird beetle species, Propylea quatuordecimpunctata and Hippodamia variegata.

Comparative data for the reproductive responses to different photoperiod conditions for H. variegata were taken from Obrycki (2018). Additionally, comparisons of responses to the four photoperiods between P. quatuordecimpunctata from Jefferson County, NY, USA to P. quatuordecimpunctata from Montreal, Quebec, Canada were also conducted. Data for the Montreal, Quebec, Canada population are from Obrycki et al., (1993).

Two Data sets are included:

(1) Pre-oviposition period (days) as a measure of induction and …

What Influences Trust In And Understanding Of Clinical Trials? An Analysis Of Information And Communication Technology Use And Online Health Behavior From The Health Information National Trends Survey, Aurora Occa, Allison S. Merritt, Allison Leip, Jerod L. Stapleton

UK CARES Faculty Publications

Background: Using information and communication technologies to seek, discuss, and share health-related information influences people’s trust and knowledge of several health practices. However, we know little about the associations between individuals’ information and communication technology use and their perceptions of trust and knowledge of clinical trials. Examining these associations may lead to the identification of target audiences and channels for developing effective educational interventions and campaigns about clinical trials.

Methods: In this study, we analyzed Health Information National Trends Survey data to document perceptions of clinical trial-related knowledge and trust that were recently added as questions in this annual national …

Cancer Research Provides A Model For Advancing Clinical Trials In Dementia In The Era Of Disease-Modifying Alzheimer’S-Type Dementia Therapies, Gregory Jicha, Thomas Tucker, Susanne Arnold, Peter T. Nelson

Neurology Faculty Publications

Dementia and cancer are multifactorial, widely-feared, age-associated clinical syndromes that are increasing in prevalence. There have been major breakthroughs in clinical cancer research leading to some effective treatments, whereas the field of dementia has achieved comparatively limited success in clinical research. The lessons of cancer research may help those in the dementia research field in confronting some of the dilemmas faced when the clinical care regimen is not entirely safe or efficacious. Cancer clinical trials have assumed that untreated individuals with cancer are at high risk for morbidity and mortality after primary diagnoses. Thus, patients deserve a choice of clinical …

Do The Sexually Dimorphic Biofluorescent Glands Of Plethodon Cinereus Function In Mate Choice And Are They Seasonally Regulated By Photoperiod?, Sam C. Moody-Wynn

Masters Theses

The novel biofluorescent glands of the Eastern Red-backed Salamander (Plethodon cinereus), have been shown to be sexually dimorphic where males have larger and greater numbers of glands than females. Interestingly, a seasonal pattern of increased numbers of fluorescent glands, which mirrors the peak in testosterone levels, has been described. Currently, the function and regulation of the fluorescent glands is unknown. I predicted that the glands function as a form of male ornamentation used in intersexual communication, and perhaps act as an honest signal of male quality. Even though the fluorescent glands are located on the underside of the tail, there …

Nalfurafine Promotes Myelination In Vitro And Facilitates Recovery From Cuprizone + Rapamycin-Induced Demyelination In Mice, Ross Van De Wetering, Rabia Bibi, Andy Biggerstaff, Sheein Hong, Bria Pengelly, Thomas E. Prisinzano, Anne C. La Flamme, Bronwyn M. Kivell

Markey Cancer Center Faculty Publications

The kappa opioid receptor has been identified as a promising therapeutic target for promoting remyelination. In the current study, we evaluated the ability of nalfurafine to promote oligodendrocyte progenitor cell (OPC) differentiation and myelination in vitro, and its efficacy in an extended, cuprizone-induced demyelination model. Primary mouse (C57BL/6J) OPC-containing cultures were treated with nalfurafine (0.6–200 nM), clemastine (0.01–100 μM), T3 (30 ng/mL), or vehicle for 5 days. Using immunocytochemistry and confocal microscopy, we found that nalfurafine treatment increased OPC differentiation, oligodendrocyte (OL) morphological complexity, and myelination of nanofibers in vitro. Adult male mice (C57BL/6J) were given a diet con- taining …

The Effect Of A Signal Peptidase Mutation On Protein Secretion By The Maize Anthracnose Pathogen Colletotrichum Graminicola, Renata Belisario

Theses and Dissertations--Plant Pathology

Colletotrichum graminicola infects maize during several phases of plant growth, resulting in anthracnose leaf blight, stalk rot, and top die-back diseases. This ubiquitous fungus has significant destructive potential and causes millions of dollars of losses annually in North America. Colletotrichum graminicola is hemibiotrophic and initially invades living host cells via biotrophic primary infection hyphae before switching to necrotrophy and inducing host cell death, host cell-wall degradation, and lesion development.

An insertional mutation in the 3’UTR region of a gene (Cpr1), which encodes a homolog of the noncatalytic SPC22/23 subunit of the endoplasmic reticulum associated signal peptidase complex, completely …

Impacts Of Anthropogenic Sedimentation On Shell-Bed Habitats In Lake Tanganyika, Africa, Michael Soreghan, Andrew Cohen, Michael M. Mcglue, Kevin M. Yeager, Emily Ryan, Alison Johns, Ishmael Kimirei

Earth and Environmental Sciences Faculty Publications

Lake Tanganyika, in central Africa, contains a diverse and endemic fauna under threat from global climate change, overfishing, and nearshore sediment pollution. Previous studies of sediment pollution focused justifiably on impacts along rocky shorelines where diversity is high, but Lake Tanganyika also contains widespread shelly accumulations (shell beds) unprecedented in the modern East African lakes, but where impacts are less con- strained. Here we integrate multiple datasets from three sites along the Tanzanian shoreline to explore how variation in sedimentation rates and sediment quality impacts shell-bed substrate and diversity and abundance of ostracodes and sponges across sites that exhibit varying …

2024 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow- calf Nutrition, Reproduction, and Management

• Impact of Increasing Level of Milk Production on Cow- Calf Performance in Nebraska Sandhills
• Artificial Insemination of Beef Heifers with Multi- Sire Semen
• Effect of Heifer Percent Mature Body Weight at Breeding on Heifer Performance, Calf Production, and Subsequent Pregnancy Rates
• Effect of Glucogenic Feed Additive on Reproductive Performance in Young Postpartum Range Cows
• The Effect of Late Gestation Supplementation Strategy on Cow- Calf Performance in March- Calving Mature Cows
• The Impact of Pre- partum Supplementation Strategy on Cow- Calf Performance in May- Calving Mature Cows
• The Effect of Postpartum Supplementation Strategy on Performance …

Predicting Live Body Wight Of Yearling Beef Heifers Using 3d Imaging, Dalton J. Anderson, Yijie Xiong, Andrea K. Watson, J. Travis Mulliniks

Nebraska Beef Cattle Reports

This study was conducted to determine the accuracy of using 3D imaging technology as a method to predict shrunk body weight (BW) of growing yearling beef heifers. Red Angus x SImmental heifers (n = 69, BW = 726 ± 62 lbs; 12 months of age) were utilized for data collection. A time-of-flight depth camera (Azure Kinect, Microsoft) was used to collect depth videos as heifers walked out of the chute. Ideal image frames were identified from videos and used to determine the body volume of each heifer. Prediction of BW using images produced an R² (estimate of model fit) …

Impact Of Varying Inclusion Of Modified Distillers Grains Plus Solubles Compared To Constant Inclusion On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca Mcdermott, Mitch M. Norman, J. C. Macdonald, Rick A. Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of varying inclusion of modified distillers grains plus solubles on a weekly basis with two inclusions of grass hay on the performance of finishing steers. no interaction was observed between modified distillers grains plus solubles inclusion (0%, 25% constant, or 25% varying from 15-35%) and hay inclusion (6% or 12%). When evaluating the effect of hay inclusion on performance, cattle fed 6% grass hay had less dry matter intake than those fed 12% grass hay, and there was a tendency for gains to be greater for cattle fed 6% grass hay. Adding …

Impact Of Feeding New Fractioinated Distillers Grains (Fiber Plus Syrup) On Feedlot Cattle Performance And Carcass Characteristics, Debora G. Sousa, Rebecca L. Mcdermott, Mitch M. Norman, James C. Macdonald, Rick Stock, Ryan A. Mass, Galen E. Erickson

Nebraska Beef Cattle Reports

Fractionation processes are being explored to supply higher protein distillers grains for premium markets. Resulting distillers grains after a fraction is isolated will still be marketed as a cattle feed so knowing the impact on performance is important. This study evaluated feeding wet or dry conventional distillers grains with wet and dry fractionated distillers (fiber plus syrup) fed at 0, 20, or 40% of diet DM. When compared to the corn control, intake and gain increased for each of the four distillers types were fed, but feed conversion was poorer (i.e., increased) for dry distillers grains and dry fiber plus …

Comparison Of Corn Silage And Earlage In Finishing Diets When Fed As A Roughage On A Neutral Detergent Fiber Basis, Garrett N. Lemon, Rebecca Mcdermott, Alfredo Dicostanzo, Galen E. Erickson, Pablo L. Loza

Nebraska Beef Cattle Reports

The finishing trial was conducted to evaluate the ability of earlage to serve as a roughage source compared to corn silage when both sources were balanced on an NDF basis. Steers were fed in a randomized block design with a 2x2 treatment arrangement, with one factor being NDF source (corn silage or earlage) and the other factor being inclusion ("normal" amount of roughage provided by corn silage or earlage as the only grain source and roughage source). No significant interactions were observed between roughage source and inclusion level on steer performance. Steers fed corn silage and grain had greater intakes …

Nebraska Producer Perceptions On The Role And Implications Of Negotiations In Fed Cattle Transactions, Taralee Hudson, Elliott James Dennis, Brad D. Lubben, James C. Macdonald

Nebraska Beef Cattle Reports

A survey of ten feelot operators, as identified through the Nebraska Department of Agriculture's (NDA) 2019-2020 Cattle Feeder's Directory was conducted to identify the important concepts related to negotiated transactions and price discovery within the fed cattle market. The surveyed feedlots account for approximately 10 percent of the cattle on feed identified within the directory, on a one time capacity basis. Conducted in April of 2023 via phone conversation, the questions were related to 1) negotiated cash transactions, 2) the bidding process, and 3) thinning cash trade. Such results lend insight into the marketing practices that currently dominate the fed …

Evaluation Of Gas Emissions From Cattle On Different Diet Adaptation Strategies Using Either Forage Or Ramp, Cindy D. Mansfield, Rebecca Mcdermott, Braden C. Troyer, Mitchell M. Norman, Maggie Youngers, Andrea K. Watson, Rick Stock, Galen E. Erickson

Nebraska Beef Cattle Reports

A 173-day finishing experiment was conducted to evaluate the effects of feeding RAMP (Cargill Corn Milling, Blair, NE) during diet adaptation compared to a traditional forage adaptation program on methane and carbon dioxide emissions, animal performance and carcass traits in beef steers. Cattle were monitored using a calorimetry emission barn to quantify production of methane and carbon dioxide during step 1 of grain adaptation and at two subsequent times while fed a common finishing diet. Feeding RAMP reduced methane by 12% during the initial diet (step 1) compared to a traditional diet that contained 43% forage. When cattle were fed …

Rethinking Corn Residue: Effects Of Grain Yield On Quality And Quantity Of Residue, Kaylee E. Wheeler, Jessica Miller, Sadie Ference, Stephan Geu, Bill Struckmeyer, Amanda Easterly, Cody F Creech, Brian Maust, Daren D. Redfearn, Jay Parsons, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A two-year experiment evaluated the effects of corn grain yield on the resulting quality and quantity of corn residue. Among the wide variety of corn hybrids, locations, and growing conditions, observed grain yields ranged from 120 to 350 bushels per acre. As corn yield increased, the total pounds of residue increased. However, the amount of reside relative to grain decreased. Within the residue, the proportion of leaf increased from 10 to 28% as corn yield increased while husk remained unchanged at 13%. Overall, the yield of leaf and husk per bushel of corn grain was not affected by grain yield …

Survey Of Current Management Practices And Evaluation Of Their Impact On Nutrient Content Of Small Cereal Silage In Nebraska, Alexa R. Johnson, Mary E. Drewnoski, Daren D. Redfearn, Susan Vanderplas, Jenny Rees, Ben Beckman, Erin Laborie, Todd Whitney, Kim Clark, Brad Schick, Gary Lesoing, Conner Biehler

Nebraska Beef Cattle Reports

The nutrient content of small cereal silage from 16 producers in Nebraska was measured at harvest and post-fermentation. At packing, 42% of the samples were below the target dry matter of 30-35%. Samples with dry matter percentage below 30% had a significant increase in the loss of energy (total digestible nutrient) content of the silage. The wetter silage appeared to have increased rates of clostridial fermentation as indicated by production of butyric acid. These data suggest that moisture management is a challenge and increased attention to ensuring the target dry matter content is achieved before packing could improve the quality …

Cereal Rye, Winter Triticale Or Winter Wheat: Which Is Best For Early Spring Grazing?, Alyssa K. Hall, Abigail M. Sartin, Kallie J. Calus, Morgan T. Grabau, Daren D. Redfearn, Robert B. Mitchell, Mary E. Drewnoski

Nebraska Beef Cattle Reports

A study was conducted to determine which winter-hardy small cereal grain was best suited for early spring grazing. Three species were evaluated: winter wheat, cereal rye, and winter triticale, as a double-cropped forage in a continuous soybean rotation. Within this rotation the number of grazing days is limited, but all three species provided high rates of cattle weight gain, with an average daily gain (ADG) of 3 lb/d in April. However, in a year where freezing conditions occurring after cattle started grazing, cattle grazing cereal rye had the greatest ADG, likely due to greater forage growth. Thus, cereal rye may …

Vitamin A In Cow-Calf Production: Impacts Of Maternal Supplementation And Status On Offspring, Hannah F. Speer, Harvey C. Freetly, Karla H. Jenkins, Mary E. Drewnoski

Nebraska Beef Cattle Reports

The young calf is at greatest risk of vitamin A deficiency when cow vitamin intake is low in late pregnancy. Two studies were conducted to evaluate the relationship between cow and calf vitamin A status and how vitamin A status of cow-calf pairs was influence by maternal vitamin A supplementation. In general, calves did not have adequate liver vitamin A concentrations despite cows having adequate liver vitamin A stores following calving. Both cow iver stores and cow vitamin A intake during late gestation influence the amount of vitamin A in colostrum, so it benefits the calf if the cow has …