Life Sciences Commons^™

Molecular Characterisation Of Traditional Cashew (Anacardium Occidentale L.) Populations From Nachingwea And Newala Districts In Tanzania, Hussein R. Chiumbi, Hashim M. Mangosongo

Tanzania Journal of Science

This study assessed the genetic diversity of 120 selected cashew plants from Nachingwea and Newala traditional cashew populations, comprising of 60 cashew plants from each population. Eight Simple Sequence Repeats (SSR) makers were used to ascertain the extent of genetic variation within and between the two traditional cashew populations. Genomic DNA extraction and Polymerase Chain Reaction (PCR) were performed using standard protocols. The results showed that all SSR markers were polymorphic. Average Polymorphic Information Content (PIC), heterozygosity, number of alleles per locus, allelic frequency and Shannon’s Index were 0.540, 0.721, 3.391, 0.666 and 0.649, respectively. Analysis of molecular variance results …

Microbial Quality Of Fresh Juices Sold By Street Vendors And Associated Human Health Risks: The Case Of Ilala Ward, Dar Es Salaam City, Tanzania, Juliana I. Rubaratuka, Godwill D. Mrema, Thomas J. Lyimo

Tanzania Journal of Science

Microbial food quality serves to indicate health risks associated with the consumption of contaminated foods. This study aimed to determine the microbial quality of fresh juices and the level of awareness and perception of the consumers and juice vendors at Ilala Ward, Dar es Salaam. Fresh samples of sugarcane, mango and mixed fruit juices were collected from four different sampling stations and analysed using standard methods for Total Coliform (TC), Faecal Coliform (FC) and Total Viable Count (TVC). The level of awareness and perception of 60 juice vendors and 60 consumers were also assessed by using questionnaires. The results showed …

Population Genetic Structure And Demographic History Of Opsaridium Microcephulum Along Lake Nyasa, Alex Nehemia, Alinanuswe J. Mwakalesi

Tanzania Journal of Science

The Sanjika, Opsaridium microcephalum is among the fish species that serve as a major source of proteins and income to people along Lake Nyasa. However, the information on its genetic diversity and structure particularly in the Tanzania part of the Lake is limited. Therefore, the assessment of the genetic diversity and structure of O.microcephalum along the Lake Nyasa part of Tanzania was conducted in the current study using a fragment of the mitochondrial COI gene from 92 individuals of O. microcephalum. The findings showed that an average haplotype diversity and average nucleotide diversity were 0.8065 ± 0.0314 and 0.002380 ± …

Heavy Metals Analysis And Physicochemical Characterization Of Groundwater At A Battery Recycling Site In South-Western Nigeria, Adeniyi T. Adeleke, Rasaki K. Odunaike, Shamsideen K. Alausa, Idris O. Olayiwola, Adetoro T. Talabi, Qasim A. Adeniji, Adejumobi C. Adeniyi

Tanzania Journal of Science

Indiscriminate dumping of battery waste is a huge issue that endangers human health and the environment. This study aimed at analysing the health impacts of exposure to pollution from spent battery recycling in Ogun State, which houses a diverse range of battery recycling industries. At this study site, forty water samples were studied over the Wet and Dry seasons to assess the impact of battery recycling waste on groundwater. Except for the TSS, the physiochemical parameters of the groundwater vary with season and are within the permissible limits. The electrical conductivity (EC), turbidity, Phosphorus, Biochemical oxygen demand (BOD), Dissolve oxygen …

Neuropsychological Malingering Determination: The Illusion Of Scientific Lie Detection, Chunlin Leonhard, Christoph Leonhard

Georgia Law Review

Humans believe that other humans lie, especially when stakes are high. Stakes can be very high in a courtroom, from substantial amounts of monetary damages in civil litigation to liberty or life in criminal cases. One of the most frequently disputed issues in U.S. courts is whether litigants are malingering when they allege physical or mental conditions for which they are seeking damages or which would allow them to avoid criminal punishment. Understandably, creating a scientific method to detect lies is very appealing to all persons engaged in lie detection. Neuropsychologists claim that they can use neuropsychological assessment tests (Malingering …

Slight Cost Of Production Changes Noted In The 2024 Nebraska Crop Enterprise Budgets, Glennis Mcmclure

Cornhusker Economics

The Nebraska crop budgets are available for the 2024 production season. The 84 enterprise budgets prepared in the October 2023 timeframe use input prices collected as of that time. Overall, estimates reflect slight cost of production decreases in the budgets when compared to the 2023 cost estimates. For 2024, the main drivers of cost decreases are due to decreased fuel and fertilizer costs. In some cases, pesticide costs decreased, yet for some of those products, prices increased. Seed and interest costs have increased for 2024 adding to cash expenses, while land opportunity costs and depreciation costs have continued to rise …

Evaluation Of The Modulatory Effects Of Capsicum Chinensis Methanol Extract In Streptozotocin-Induced Diabetic Neuropathic Pain In Mice, Babatunde A. Alabi, Adeyinka Aderonmu, Sodiq K. Lawal, Okot-Asi T. Nku-Ekpang, Mayowa Ajayi, Olugbenga E. Iwalewa

Tanzania Journal of Science

Diabetes is one of the common causes of neuropathic pain. The first-line treatment drugs used in alleviating neuropathic pain can cause adverse effects. Therefore, the aim of this study is to evaluate the analgesic property of Capsicum chinensis, since the plant was reported to be used against chronic pain in traditional medicine. Streptozotocin-induced diabetes was adopted to model neuropathy in mice and the antihyperalgesic effect of the extract was evaluated using a hot plate test, cold allodynia test, and Randall- Selitto paw pressure test. The plasma level of pain-associated inflammatory biomarkers like interleukin-6 (IL-6) and tissue necrotic factor-α (TNF-α) was …

Woody Plant Assemblages Of Recently Declared Village Land Forest Reserve In The Eastern Afromontane Biodiversity Hotspot, Samora Macrice Andrew

Tanzania Journal of Science

Understanding of species composition of village land forest reserves and their driving factors informs adequate development of effective restoration strategies and sustainable forest management of miombo woodlands. This study assessed the effects of human disturbance as well as environmental variables on woody plant species composition using 24 square plots of 10 x 10 m in a recently declared village land forest reserve in the Eastern Afromontane biodiversity hotspot of Tanzania. Ordination analysis technique canonical correspondence analysis was used to identify important vegetation gradients and significant factors that explain the spatial variation in species composition of woody plants. Results showed that, …

Woody Species Composition, Structure, Regeneration Status And Carbon Storage Of Mkulazi Forest Reserve In Tanzania, Ezekiel E. Mwakalukwa, Christoganus John, Paulo J. Lyimo

Tanzania Journal of Science

Little is known about the status of forest condition and carbon storage potential of Mkulazi forest reserve (MFR) located in Morogoro District, Tanzania. This study was conducted to assess i) woody species composition, richness and diversity, ii) stand structure, iii) regeneration status and iv) carbon storage. Data collection for vegetation involved establishment of 100 concentric circular sample plots in the forest area of 65,710 ha. A total of 54 plant species for trees and shrubs with DBH ≥ 5 cm that belongs to 20 plant families were identified. The diversity of woody species was high (H’ = 3.11), while Stem …

Gene Flow Between The Wild Rice Species (Oryza Longistaminata) And Two Varieties Of Cultivated Rice (Oryza Sativa) In Kilombero District, Tanzania, Hashim M. Mangosongo, Emmarold E. Mneney, Bramwel Wanjala

Tanzania Journal of Science

The direction and rate of gene flow between the perennial wild rice species, O. longistaminata and cultivated rice, O. sativa was investigated using nine Microsatellite (SSR) markers. The study involved characterization of parental lines of two cultivated rice varieties and O. longistaminata and their F1 progenies. Presence of crop-specific alleles in the wild species or vice-versa was used as an indicator of occurrence of gene flow between the two species. The study revealed that gene flow between O. longistaminata and each of the two varieties of O. sativa occurs naturally. The direction of gene flow was mainly from the cultivated …

Potential Of Endophytic Fungal Isolates In Improving Productivity And Postharvest Marketable Fruit Qualities Of Tomato, Gabriel Michael, Emmanuel M. Sangu

Tanzania Journal of Science

In Tanzania, low tomato (Solanum lycopersicum) yield and postharvest marketable fruit qualities remain significant limitations for tomato grower’s profit. Seven endophytic fungal isolates from pyrethrum and Lemongrass leaf and or flowers were evaluated to promote tomato productivity and fruit qualities. The isolates were inoculated by priming the seeds in spore suspension, and the variations in the number of germinated seeds, seedling vigour, yield and fruit qualities were compared across the treatments. It was observed that only three isolates induced more than 50% seed germination. Epf1 and Epl1 isolates showed significant promotion of seed germination (F 0.05 (pdf, 23) = 8.121 …

Potential Of Endophytic Fungal Isolates In Improving Productivity And Postharvest Marketable Fruit Qualities Of Tomato, Gabriel Michael, Emmanuel M. Sangu

Tanzania Journal of Science

In Tanzania, low tomato (Solanum lycopersicum) yield and postharvest marketable fruit qualities remain significant limitations for tomato grower’s profit. Seven endophytic fungal isolates from pyrethrum and Lemongrass leaf and or flowers were evaluated to promote tomato productivity and fruit qualities. The isolates were inoculated by priming the seeds in spore suspension, and the variations in the number of germinated seeds, seedling vigour, yield and fruit qualities were compared across the treatments. It was observed that only three isolates induced more than 50% seed germination. Epf1 and Epl1 isolates showed significant promotion of seed germination (F 0.05 (pdf, 23) = 8.121 …

Synthesis Of Template-Free Flower-Like Zno Nanorods Using A Simple Chemical Bath Technique, Benard S. Mwankemwa

Tanzania Journal of Science

Flower-like ZnO nanorods were successfully synthesised using a template-free, low-temperature chemical bath deposition method. The effects of growth time on the structural, morphological, and vibrational properties of flower-like zinc oxide were investigated. X-ray diffraction (XRD) and scanning electron microscopy (SEM) indicated that the crystallite size and density of flower-like ZnO nanorods increased with increasing growth time. Raman spectroscopy confirmed the existence of specific Raman modes that correspond to the wurtzite structure of ZnO. In addition, Raman measurements revealed that increased growth time increases the Raman modes intensity of the flower-like ZnO nanorods. Moreover, the measurements of the Au/ZnO nanorods/FTO Schottky …

Antibacterial And Anticancer Activities Of A Fosmid Clone From Atlantis Ii Red Sea Brine Pool., Marina Nabil

Theses and Dissertations

Antimicrobial resistance (AMR) and cancer are among the top global human health issues requiring urgent control practices. AMR caused 5 million death cases around the world in 2019. This number is expected to reach 10 million deaths by 2050. Cancer deaths reached 10 million in 2020. Additionally, anticancer drug resistance is a common cause of death among cancer patients. Screening for novel compounds is one of the strategies recommended by the World Health Organization (WHO) to control drug resistance in microbial and cancer cells. Marine natural products (MNPs) are characterized by chemically diverse structures with various bioactivities, especially in extreme …

Phosphomolybdic Acid-Doped Zeolitic Imidazolate Framework-8 Hybrid: Nanostructured Material With Synergistic Antibacterial Effect, Mariam Mohammad Abdelkhalek

Theses and Dissertations

Bacterial infections represent a major global health concern, causing millions of deaths and significant economic burdens. The development of nanostructured surfaces with antibacterial activity can revolutionize infection control practices. In this study, a hybrid material of Zeolitic imidazolate framework-8 (ZIF-8) doped with phosphomolybdic acid (PMA) was synthesized and characterized by FESEM, EDS, XRD, FTIR, and N2 sorption isotherm. The antibacterial activity against Escherichia coli as a model organism was tested by colony forming unit (CFU) reduction assay, minimum inhibitory concentration (MIC), and time-kill curve. PMA@ZIF-8 performance as an antibacterial agent was superior to its individual constituents, suggesting synergistic effect of …

Cytoskeleton Remodeling Induced By Smyd2 Methyltransferase Drives Breast Cancer Metastasis, Alexandre G Casanova, Gael S Roth, Simone Hausmann, Xiaoyin Lu, Ludivine J M Bischoff, Lucid Belmudes, Natasha M Flores, Ana Morales Benitez, Tourkian Chasan, Marcello Caporicci, Jessica Vayr, Sandrine Blanchet, Francesco Ielasi, Sophie Rousseaux, Pierre Hainaut, Or Gozani, Muriel Le Romancer, Yohann Couté, Andres Palencia, Pawel K Mazur, Nicolas Reynoird

Faculty, Staff and Student Publications

Malignant forms of breast cancer refractory to existing therapies remain a major unmet health issue, primarily due to metastatic spread. A better understanding of the mechanisms at play will provide better insights for alternative treatments to prevent breast cancer cell dispersion. Here, we identify the lysine methyltransferase SMYD2 as a clinically actionable master regulator of breast cancer metastasis. While SMYD2 is overexpressed in aggressive breast cancers, we notice that it is not required for primary tumor growth. However, mammary-epithelium specific SMYD2 ablation increases mouse overall survival by blocking the primary tumor cell ability to metastasize. Mechanistically, we identify BCAR3 as …

Measures Of Resting-State Brain Network Segregation And Integration Vary In Relation To Data Quantity: Implications For Within And Between Subject Comparisons Of Functional Brain Network Organization, Liang Han, Micaela Y Chan, Phillip F Agres, Ezra Winter-Nelson, Ziwei Zhang

Faculty, Staff and Student Publications

Measures of functional brain network segregation and integration vary with an individual's age, cognitive ability, and health status. Based on these relationships, these measures are frequently examined to study and quantify large-scale patterns of network organization in both basic and applied research settings. However, there is limited information on the stability and reliability of the network measures as applied to functional time-series; these measurement properties are critical to understand if the measures are to be used for individualized characterization of brain networks. We examine measurement reliability using several human datasets (Midnight Scan Club and Human Connectome Project [both Young Adult …

Management Of Infliximab Refractory Immune Checkpoint Inhibitor Gastrointestinal Toxicity: A Multicenter Case Series, Catriona Harvey, Kazi J Nahar, Janet Mckeown, Serigne N Lo, Sheima Farag, Kate Young, Aafke Meerveld-Eggink, Christian Blank, Austin Thomas, Jennifer Mcquade, Bastian Schilling, Douglas B Johnson, Roberto Martín Huertas, Ana Arance, Joanna Lee, Lisa Zimmer, Georgina V Long, Matteo S Carlino, Yinghong Wang, Alexander Maxwell Menzies

Faculty, Staff and Student Publications

Background: Immune checkpoint inhibitor (ICI) gastrointestinal toxicity (gastritis, enteritis, colitis) is a major cause of morbidity and treatment-related death. Guidelines agree steroid-refractory cases warrant infliximab, however best management of infliximab-refractory ICI gastrointestinal toxicity (IRIGItox) is unknown.

Methods: We conducted an international multicenter retrospective case series. IRIGItox was defined as failure of symptom resolution ≤grade 1 (Common Terminology Criteria for Adverse Events V.5.0) following ≥2 infliximab doses or failure of symptom resolution ≤grade 2 after one dose. Data were extracted regarding demographics, steroid use, response to treatment, and survival outcomes. Toxicity was graded at symptom onset and time of infliximab failure. …

A Novel Polymer-Encapsulated Multi-Imaging Modality Fiducial Marker With Positive Signal Contrast For Image-Guided Radiation Therapy, Li Wang, Jeremiah Sanders, John F Ward, Stephen R Lee, Falk Poenisch, Narayan Sahoo, Jingfei Ma, Rajat J Kudchadker, Seungtaek L Choi, Quynh-Nhu Nguyen, Lauren L Mayo, Shalin J Shah, Steven J Frank

Faculty, Staff and Student Publications

BACKGROUND: Current fiducial markers (FMs) in external-beam radiotherapy (EBRT) for prostate cancer (PCa) cannot be positively visualized on magnetic resonance imaging (MRI) and create dose perturbation and significant imaging artifacts on computed tomography (CT) and MRI. We report our initial experience with clinical imaging of a novel multimodality FM, NOVA.

METHODS: We tested Gold Anchor [G-FM], BiomarC [carbon, C-FM], and NOVA FMs in phantoms imaged with kilovoltage (kV) X-rays, transrectal ultrasound (TRUS), CT, and MRI. Artifacts of the FMs on CT were quantified by the relative streak artifacts level (rSAL) metric. Proton dose perturbations (PDPs) were measured with Gafchromic EBT3 …

Safety, Immunogenicity, And Mechanism Of A Rotavirus Mrna-Lnp Vaccine In Mice, Qingmei Leng, Xiaopeng Song, Xiaochen Lin, Jun Ye, Jinlan Wang, Jinmei Li, Lida Yao, Xianqiong Tang, Xiangjun Kuang, Guangming Zhang, Maosheng Sun, Yan Zhou, Hongjun Li

Faculty, Staff and Student Publications

Rotaviruses (RVs) are a major cause of diarrhea in young children worldwide. The currently available and licensed vaccines contain live attenuated RVs. Optimization of live attenuated RV vaccines or developing non-replicating RV (e.g., mRNA) vaccines is crucial for reducing the morbidity and mortality from RV infections. Herein, a nucleoside-modified mRNA vaccine encapsulated in lipid nanoparticles (LNP) and encoding the VP7 protein from the G1 type of RV was developed. The 5' untranslated region of an isolated human RV was utilized for the mRNA vaccine. After undergoing quality inspection, the VP7-mRNA vaccine was injected by subcutaneous or intramuscular routes into mice. …

The Effect Of Pornography On Marriage And Its Societal Impacts: A Neurobiological Perspective, Katie Rose Geer

NEXUS: The Liberty Journal of Interdisciplinary Studies

No abstract provided.

The Intersection Of Theatre And Cognitive Neuroscience, Mary Katherine Flage

NEXUS: The Liberty Journal of Interdisciplinary Studies

No abstract provided.

Bioethics Of Human Cloning: Are We Playing God?, Caleb Smith, Mary Katherine Flage

NEXUS: The Liberty Journal of Interdisciplinary Studies

No abstract provided.

Effects Of Sodium Hypochlorite On The Physicochemical Characteristics Of Arrowhead Starch, Xi Wanting, Liu Guiru, Li Meng, Ji Derong, Duan Lili

Food and Machinery

Objective: The effect of sodium hypochlorite on the physicochemical properties and digestive characteristics of arrowhead starch was studied. Methods: In a weakly alkaline environment, sodium hypochlorite was used as an oxidant, and the added amounts were 0%, 1%, 3%, 5%, 7%, and 9% (based on the mass fraction of available chlorine in dry basis arrowhead starch). Following a 4-hour oxidation reaction with arrowhead starch, the modified starch's amylose content, swelling power, solubility, coagulation stability, color difference, light transmittance, scanning electron microscopy, X-ray diffraction, and other physical and chemical properties were measured, along with its digestibility. Results: The amylose concentration, solubility …

Design Of Intelligent Air Screen System Based On Fuzzy Control Algorithm, Liu Hongpeng, Lin Yuliang, Tian Zhongcai, Fang Wenjing

Food and Machinery

Objective: To solve the technical problems such as low separation efficiency, unstable control effect and overshoot in crop screening by air screen. Methods: An intelligent air screen system based on fuzzy control algorithm was proposed. The wind speed and the change rate of wind speed were designed as input variables, and the steering gear angle was taken as output variable. The angle of the steering gear was adjusted by fuzzy control rules to reduce the overshot of the screen, so as to improve the screening efficiency of crop impurities. Results: Through the system test, under the condition of 3 kg/s …

Effect Of Selenium-Enriched Yeast On The Quality Of Coix Seed Jiulao, Lei Juanjuan, Liang Ling, Huang Qingeng

Food and Machinery

Objective: This study aimed to evaluate the effects of selenium-enriched yeast on the quality of coix seed jiulao. Methods: The coix seed was used as raw material, which was inoculated with selenium-rich saccharomyces cerevisiae to prepare coix seed jiulao. The changes of physicochemical properties, plant active components and in vitro antioxidant activity were measured during the fermentation of jiulao. Results: Compared with the S. cerevisiae group, the selenium-enriched S. cerevisiae group enhanced the improvement rate of alcohol content. It also accelerated the decrease rate of pH and total sugar content in the middle stage (12~30 h) of coix seed jiulao …

Research Progress On The Effects Of Ultrafine Grinding Technology On Nutrients And Specific Physiological Functions Of Grain Products, Yang Lei, Jia Yanju, Liu Jingke, Sheng Qinghai, Wang Yunting

Food and Machinery

Ultrafine grinding is an advanced technology that can grind materials down to the micron or even nanometer level, with significant advantages of material savings, fast grinding speed, and uniform and fine powder particle size. Recently, it has gradually been applied in the pre-treatment of grain crops, which has positive significance for the production of food with good taste, a high nutritional material dissolution rate, and strong functionality. This review summarizes the classification, advantages, and disadvantages of 7 different types of ultrafine grinding technology. The effect of ultrafine grinding technology on the nutrients (protein, starch, fat, dietary fiber and phenolic compound) …

Effects Of Two-Step Heating Process On Hydrophobicity And Barrier Properties Of Protein-Based Composites, Wang Zi, Lei Qiao, Zhang Wenhui

Food and Machinery

Objective: To improve the film heating process. Methods: In this experiment, edible protein-polysaccharide films were prepared by using the method of chain dissociation at 35, 40, 45, 50, 55 ℃ at one stage and denaturing and heating whey protein isolation at 85 ℃ at the second stage. The structure, hydrophobicity, barrier properties and mechanical properties of the films were compared, and the optimum film forming process conditions were determined. Results: The oxygen resistance, tensile strength （TS） and elongation at break （EAB） of the film increased by 25.0%~58.7%, 54.8%~89.9% and 40.2%~59.2%, respectively, when the film was heated at 25 ℃ and …

Quantum Chemistry Calculation Of Antioxidant Synergistic Effect Of Resveratrol And Sesamol In Oil, Gao Weihong, Qu Xiaodi, Yao Yunping, Li Changmo

Food and Machinery

Objective: This study focused on the antioxidant synergistic mechanism between resveratrol and sesamol. Methods: The levels of free radicals and peroxide free radicals in sunflower seed oil were assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide assays, respectively. The proportion of resveratrol and sesamol were optimized by integrating the measurements with the determination of the induction period. Additionally, quantum chemical simulations were employed to elucidate the underlying reaction processes. Results: Upon heating sunflower seed oil supplemented with resveratrol and sesamol at 180 ℃ for 2 hours, the total content of free radicals was determined as （0.08±0.03） mol/L and （0.20±0.03） mol/L, …

Study On The Adsorption Properties Of Nano-Calcium Carbonate Surface Modified Zeolite To Phenolic Acid In Simulated Cane Juice, Liu Fujie, Huang Shihui, Pan Lili

Food and Machinery

Objective: This study aimed to explore the adsorption performance and mechanism of phenolic acids in simulated sugarcane juice by nano-calcium carbonate modified zeolite (CaCO₃@ZL). Methods: Using zeolite as the supporting framework, a co-precipitation technique was utilized to create nano-calcium carbonate modified zeolite (CaCO₃@ZL), which was then employed to simulate the adsorption of phenolic acids in sugarcane juice. Using X-ray diffraction (XRD) and infrared spectroscopy (FTIR), the zeolite's characteristics were examined both before and after alteration. The kinetic model fitting of CaCO₃@ZL and static adsorption studies were also conducted. The properties of phenolic acids were …