Life Sciences Commons^™

Influence Of The International Journal Of Exercise Science, Duane V. Knudson, Gracie K. Burson

International Journal of Exercise Science

International Journal of Exercise Science 17(2): 265-273, 2024. The International Journal of Exercise Science (IJES) publishes research from numerous subdisciplines of exercise science and health. This study documented the scholarly influence of the initial 15-year history (2008-2022) of the IJES. Publication, indexing, from the IJES website and four database services: Dimensions, Google Scholar (GS), PubMed, and SCImago Journal & Rank. The IJES has published 1055 articles in 79 issues in the first 15 years. The top 106 (10%) cited articles received a total of 7,195 citations according to GS. Top-cited IJES articles …

Acute Effects In Postural Sway As A Result Of Self-Myofascial Release On The Lower Extremities In Collegiate Female Athletes., Mackenzie Melton, Andrew Shim, Monica Dial, Guilherme M. Cesar

International Journal of Exercise Science

International Journal of Exercise Science 17(1): 274-284, 2024. Myofascial release is a popular therapy technique used to manipulate connective muscle tissue to become more pliable. The maintenance of body posture relies on mechanoreceptors located in connective tissue, thus manipulation of connective tissue should affect postural control. The effects of this phenomenon have not been well studied, leaving room for this investigation. PURPOSE: To observe if postural sway scores changed before and after foam rolling proximal (quadriceps and hamstrings) in comparison to distal (calves) muscles. METHODS: Thirty-six, college-aged female athletes (age 20.39 ± 0.25 years, mass 68.70 ± 1.97 kg, height …

The Transmission Of Oropharyngeal Squamous Cell Carcinoma, Kunjal Patel, Aleesha Thomas

Mako: NSU Undergraduate Student Journal

The existence of Oropharyngeal Squamous Cell Carcinoma (OPSCC) has recently been found to have correlations with the Human Papillomavirus. HPV-associated OPSCC exhibits a unique method of infection and transmission and has made this branch an emerging disease in the recent decade. This systematic review of the literature was conducted to further explore research into Oropharyngeal Squamous Cell Cancer (OPSCC). Commonly referred to as “throat cancer”, this growth originates in the oropharynx. Symptoms of this condition include sore throat, lumps in the neck, and difficulty with swallowing. OPSCC has many variants but has shown a strong association with Human Papillomavirus (HPV), …

Zika Virus Microcephaly: Predicting The Symptoms Of Microcephaly So Families Can Prepare, Eleanor J. Wind

Mako: NSU Undergraduate Student Journal

Microcephaly is a rare congenital defect where the circumference of an infants head is smaller than average. The cause is unknown and the risk factors and symptoms of microcephaly are wide ranging. One risk factor is exposure during pregnancy to viruses such as the Zika virus.. A recent study was conducted on infants who were born with Zika virus Microcephaly following the Zika outbreak in Brazil. The researchers conducted MRI scans and developmental testing. Then an algorithm to predict behavioral outcomes of Zika Virus Microcephaly was successfully created. This research will benefit caretakers of infants with Zika virus Microcephaly by …

History Of Clover Leaf Syndrome, Isabella Perez

Mako: NSU Undergraduate Student Journal

The purpose of this paper is to summarize the history of clover leaf syndrome and describe the newest advancements made to treat it. Clover leaf syndrome is more formally referred to as Kleeblattschadel syndrome. Information was gathered from several scholarly, peer-reviewed articles, and was condensed down into the key takeaways. This syndrome impacts the formation of the skull due to premature fusion of its sutures, creating a tri-lobar skull that resembles a clover leaf. This premature fusion is referred to as a type of craniosynostosis and has been linked to causing several other health complications ranging in severity. This is …

Recent Studies In The Surgical Treatment Of Tetralogy Of Fallot, Emma C. Gardner

Mako: NSU Undergraduate Student Journal

No abstract provided.

Encephaloceles: A Comprehensive Exploration Of Research, Causes, Prevention, And Innovative Approaches To Diagnosis And Treatment, Amanda N. Bautista

Mako: NSU Undergraduate Student Journal

Encephaloceles, a rare medical condition derived from Greek words meaning "brain hernia," involve the protrusion of brain tissue, cerebrospinal fluid, or membranes through a defect in the skull. These anomalies often present at birth and can occur in various locations on the skull. Recent research has shed light on the possible causes of encephaloceles, including genetic factors such as the Sonic Hedgehog pathway and inadequate folic acid intake during pregnancy. Folic acid is essential for proper neural tube closure during fetal development, making it a key preventive measure.

Emerging treatments for encephaloceles show promise, with a focus on mesenchymal stem …

The Emerging Role Of Non-Coding Rnas (Ncrnas) In Plant Growth, Development, And Stress Response Signaling, Amit Yadav, Jyotirmaya Mathan, Arvind Kumar Dubey, Anuradha Singh

Center for Plant Science Innovation: Faculty Publications

Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play a crucial regulatory role in plant biology, surpassing the essential functions of RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent an emerging class of regulators, operating directly in the form of small interfering RNAs (siRNAs), microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). These ncRNAs exert control at various levels, including transcription, posttranscription, translation, and epigenetic. Furthermore, they interact with each other, contributing to a variety of biological processes and mechanisms associated with stress resilience. …

Are Animals Capable Of Using Human Language?, Shiree Karim, Elyse C. Tancini

CAFE Symposium 2024

A study focusing on animals' capability to learn and use human language for communication, understanding the process of language acquisition, the history and evolution of language, alongside findings from experiments involving gorillas, bonobos, parrots and horses.

Embracing Diversity In Agricultural Economics, Timothy Meyer

Cornhusker Economics

To steal an overused cliché, “There’s room in the tent for everyone.” Over the 2023 academic year, I have reiterated this message to all my students, with one addition. Not only is there room for everyone, but all are invited AND welcome. Food is something we all have in common, no matter the background. I think this is why producers in the state of Nebraska feel as strongly as they do about the food they produce; it is life-giving and should be taken seriously. Nebraska Agriculture is part of what makes our state great, and that is not a secret …

Fear, Racism, Agriculture: The Drive For Japanese Internment, Brandon James March

Doctoral Dissertations and Projects

The focus of this dissertation is the timing of the forced evacuation of the ethnic Japanese population from the West Coast in 1942. This work focuses on three key factors driving the timing of the evacuation: racism, security concerns, and agriculture. Racism has been studied and written about extensively; however, an overview of this factor is critical as it directly influenced the removal of Japanese American citizens in addition to Japanese immigrants. This dissertation will focus on the intellectual origins of racism and prejudice by focusing on key figures and tracing the ideas and beliefs and how they influenced the …

Diaphragmatic Breathing Influences Recovery Metrics After Aerobic Exercise, Trace L. Cruz

Masters Theses

Recovery from aerobic exercise is multifaceted and highly complex. Recovery includes cellular repair, substrate availability, autonomic nervous system status, and many other factors that comprise homeostasis. The present study is designed to assess the influence of diaphragmatic breathing after aerobic exercise to exhaustion. Diaphragmatic breathing is deep rhythmical breathing that is known to influence the autonomic nervous system, specifically through cranial nerve X (Vagus nerve). Subjects (n=12, age = 22.3±1.15 years old, height = 67.882.17 inches, weight =154.4515.20 pounds, BF% = 15.785.43). Performed four VO2max tests on two different days (2 each day). On each day, subjects performed a 20:00 …

Rapid Anti-Myeloma Activity By T Cells Expressing An Anti-Bcma Car With A Human Heavy-Chain-Only Antigen-Binding Domain, Jennifer Mann, Jennifer N Brudno, Stephanie L Goff, James C Yang, Micaela Ganaden, Rashmika Patel, Zhili Zheng, Jared J Gartner, Kathryn R Martin, Hao-Wei Wang, Constance M Yuan, Tyler Lowe, Irina Maric, Lipei Shao, Ping Jin, David F Stroncek, Steven L Highfill, Steven A Rosenberg

Faculty, Staff and Student Publications

Multiple myeloma (MM) is a rarely curable malignancy of plasma cells. MM expresses B cell maturation antigen (BCMA). We developed a fully human anti-BCMA chimeric antigen receptor (CAR) with a heavy-chain-only antigen-recognition domain, a 4-1BB domain, and a CD3ζ domain. The CAR was designated FHVH33-CD8BBZ. We conducted the first-in-humans clinical trial of T cells expressing FHVH33-CD8BBZ (FHVH-T). Twenty-five patients with relapsed MM were treated. The stringent complete response rate (sCR) was 52%. Median progression-free survival (PFS) was 78 weeks. Of 24 evaluable patients, 6 (25%) had a maximum cytokine-release syndrome (CRS) grade of 3; no patients had CRS of greater …

The Hsp90-Myc-Cdk9 Network Drives Therapeutic Resistance In Mantle Cell Lymphoma, Yang Liu, Qingsong Cai, Yu Xue, Yijing Li, Joseph Mcintosh, Zhihong Chen, Jovanny Vargas, Lei Nie, Yixin Yao, Heng-Huan Lee, Wei Wang, Johnnelson R Bigcal, Maria Badillo, Jitendra Meena, Christopher Flowers, Jia Zhou, Zhongming Zhao, Lukas M Simon, Michael Wang

Faculty, Staff and Student Publications

Brexucabtagene autoleucel CAR-T therapy is highly efficacious in overcoming resistance to Bruton's tyrosine kinase inhibitors (BTKi) in mantle cell lymphoma. However, many patients relapse post CAR-T therapy with dismal outcomes. To dissect the underlying mechanisms of sequential resistance to BTKi and CAR-T therapy, we performed single-cell RNA sequencing analysis for 66 samples from 25 patients treated with BTKi and/or CAR-T therapy and conducted in-depth bioinformatics™ analysis. Our analysis revealed that MYC activity progressively increased with sequential resistance. HSP90AB1 (Heat shock protein 90 alpha family class B member 1), a MYC target, was identified as early driver of CAR-T resistance. CDK9 …

Transcription Factor Bach1 In Cancer: Roles, Mechanisms, And Prospects For Targeted Therapy, Junqing Jiang, Jiaqian Zhang, Zhangfan Wu, Yijun Wang, Mengyu Sun, Xiaoping Chen, Bixiang Zhang, Xiao Xu, Shuai Wang, Shengjun Xu, Yufei Wang, Wenjie Huang, Limin Xia

Faculty, Staff and Student Publications

Transcription factor BTB domain and CNC homology 1 (BACH1) belongs to the Cap 'n' Collar and basic region Leucine Zipper (CNC-bZIP) family. BACH1 is widely expressed in mammalian tissues, where it regulates epigenetic modifications, heme homeostasis, and oxidative stress. Additionally, it is involved in immune system development. More importantly, BACH1 is highly expressed in and plays a key role in numerous malignant tumors, affecting cellular metabolism, tumor invasion and metastasis, proliferation, different cell death pathways, drug resistance, and the tumor microenvironment. However, few articles systematically summarized the roles of BACH1 in cancer. This review aims to highlight the research status …

Patient And Dentist Perspectives On Collecting Patient Reported Outcomes After Painful Dental Procedures In The National Dental Pbrn, Rahma Mungia, Alfa-Ibrahim Yansane, David Holmes, Kim Funkhouser, Ana M Ibarra-Noriega, Janelle Urata, D Brad Rindal, Heiko Spallek, Joel White, Muhammad F Walji

Faculty, Staff and Student Publications

BACKGROUND: Dental Patient Reported Outcomes (PROs) relate to a dental patient's subjective experience of their oral health. How practitioners and patients value PROs influences their successful use in practice.

METHODS: Semi-structured interviews were conducted with 22 practitioners and 32 patients who provided feedback on using a mobile health (mHealth) platform to collect the pain experience after dental procedures. A themes analysis was conducted to identify implementation barriers and facilitators.

RESULTS: Five themes were uncovered: (1) Sense of Better Care. (2) Tailored Follow-up based on the dental procedure and patient's pain experience. (3) Effective Messaging and Alerts. (4) Usable Digital Platform. …

Evidence That Aquaporin 11 (Aqp11) In The Spiny Dogfish (Squalus Acanthias) May Represent A Pseudogene, Christopher P. Cutler, Meghan Canicatti, Esosa Omoregie

Biology: Faculty Publications

Various attempts to amplify an AQP11 cDNA from tissues of the spiny dogfish (Squalus acanthias) were made. Two pairs of deoxy-inosine-containing degenerate primers were designed based on conserved amino acid sequences from an AQP11 alignment. These primers yielded some faint bands from gill cDNA that were sequenced. Blast searches with the sequences showed they were not AQP11. An elasmobranch AQP11 nucleotide sequence alignment was produced to identify conserved regions to make further degenerate primers. One primer pair produced a short 148 bp fragment showing particularly strong amplification in gill and intestine. It was sequenced and represented a piece of the …

Stromal Reprogramming By Fak Inhibition Overcomes Radiation Resistance To Allow For Immune Priming And Response To Checkpoint Blockade, Varintra Lander

Arts & Sciences Graduate Student Theses and Dissertations

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal malignancies. While immune checkpoint blockade (ICB) is effective in many solid cancers, it has not been effective in PDAC. Furthermore, clinical trials combining ICB with standard of care chemo- or radio-therapy (RT), which should be able to prime anti-tumor immunity and unlock ICB, have not been overwhelmingly successful. Thus, understanding the effect of RT on PDAC tumor immunity is critical to plan better combination treatment strategies for this highly recalcitrant cancer. To elucidate if RT can prime antigen specific T cell responses, we first looked at human PDAC resections. In …

Antibody-Conjugated Polymersomes With Encapsulated Indocyanine Green J-Aggregates And High Near-Infrared Absorption For Molecular Photoacoustic Cancer Imaging, Mohammed R Kawelah, Sangheon Han, Ceren Atila Dincer, Jongyeong Jeon, Joel Brisola, Ananthakrishnan S Jeevarathinam, Alexander E Marras, Thomas M Truskett, Konstantin V Sokolov, Keith P Johnston

Faculty, Staff and Student Publications

Imaging plays a critical role in all stages of cancer care from early detection to diagnosis, prognosis, and therapy monitoring. Recently, photoacoustic imaging (PAI) has started to emerge into the clinical realm due to its high sensitivity and ability to penetrate tissues up to several centimeters deep. Herein, we encapsulated indocyanine green J (ICGJ) aggregate, one of the only FDA-approved organic exogenous contrast agents that absorbs in the near-infrared range, at high loadings up to ∼40% w/w within biodegradable polymersomes (ICGJ-Ps) composed of poly(lactide-

Aspscr1-Tfe3 Reprograms Transcription By Organizing Enhancer Loops Around Hexameric Vcp/P97, Amir Pozner, Li Li, Shiv Prakash Verma, Shuxin Wang, Jared J Barrott, Jamie S E Yu, Shane Colborne, Christopher S Hughes, Ju-Fen Zhu, Sydney L Lambert, Lara S Carroll, Kyllie Smith-Fry, Michael G Stewart, Sarmishta Kannan, Bodrie Jensen, Cini M John, Saif Sikdar, Hongrui Liu, Ngoc Ha Dang, Jennifer Bourdage, Jinxiu Li, Jeffery M Vahrenkamp, Katelyn L Mortenson, John S Groundland, Rosanna Wustrack, Donna L Senger, Franz J Zemp, Douglas J Mahoney, Jason Gertz, Xiaoyang Zhang, Alexander J Lazar, Martin Hirst, Gregg B Morin, Torsten O Nielsen, Peter S Shen, Kevin B Jones

Faculty, Staff and Student Publications

The t(X,17) chromosomal translocation, generating the ASPSCR1::TFE3 fusion oncoprotein, is the singular genetic driver of alveolar soft part sarcoma (ASPS) and some Xp11-rearranged renal cell carcinomas (RCCs), frustrating efforts to identify therapeutic targets for these rare cancers. Here, proteomic analysis identifies VCP/p97, an AAA+ ATPase with known segregase function, as strongly enriched in co-immunoprecipitated nuclear complexes with ASPSCR1::TFE3. We demonstrate that VCP is a likely obligate co-factor of ASPSCR1::TFE3, one of the only such fusion oncoprotein co-factors identified in cancer biology. Specifically, VCP co-distributes with ASPSCR1::TFE3 across chromatin in association with enhancers genome-wide. VCP presence, its hexameric assembly, and its …

Androgen Drives Melanoma Invasiveness And Metastatic Spread By Inducing Tumorigenic Fucosylation, Qian Liu, Emma Adhikari, Daniel K Lester, Bin Fang, Joseph O Johnson, Andrea T Mockabee-Macias, Kelly M Guzman, Michael G White, John M Koomen, Jennifer A Wargo, Jane L Messina, Jianfei Qi, Eric K Lau

Faculty, Staff and Student Publications

Melanoma incidence and mortality rates are historically higher for men than women. Although emerging studies have highlighted tumorigenic roles for the male sex hormone androgen and its receptor (AR) in melanoma, cellular and molecular mechanisms underlying these sex-associated discrepancies are poorly defined. Here, we delineate a previously undisclosed mechanism by which androgen-activated AR transcriptionally upregulates fucosyltransferase 4 (FUT4) expression, which drives melanoma invasiveness by interfering with adherens junctions (AJs). Global phosphoproteomic and fucoproteomic profiling, coupled with in vitro and in vivo functional validation, further reveal that AR-induced FUT4 fucosylates L1 cell adhesion molecule (L1CAM), which is required for FUT4-increased metastatic …

Antioxidant, Antibacterial, And Molecular Docking Of Methyl Ferulate And Oleic Acid Produced By Aspergillus Pseudodeflectus Aumc 15761 Utilizing Wheat Bran, Ahmed Mohamed Ahmed Ali Ali Ramadan, Sabry Ahmed Hussein Zidan, Reda Mohamed Shehata, Hussein Hosny El-Sheikh, Fuad Ameen, Steven L. Stephenson, Osama Abdel-Hafeez Mohamed Al-Bedak

Biological Sciences Faculty Publications and Presentations

Secondary metabolites (SMs) are the primary source of therapeutics and lead chemicals in medicine. They have been especially important in the creation of effective cures for conditions such as cancer, malaria, bacterial and fungal infections, neurological and cardiovascular problems, and autoimmune illnesses. In the present study, Aspergillus pseudodeflectus AUMC 15761 was demonstrated to use wheat bran in solid state fermentation (SSF) at optimum conditions (pH 7.0 at 30 °C after 10 days of incubation and using sodium nitrate as a nitrogen source) to produce methyl ferulate and oleic acid with significant antioxidant and antibacterial properties. Gas chromatography-mass spectrometry (GC–MS) analysis …

Exploring Definitions Of Daily Enteric Methane Emission Phenotypes For Genetic Evaluations Using A Population Of Indoor-Fed Multi-Breed Growing Cattle With Feed Intake Data, Clodgah V. Ryan, Thierry Pabiou, Deirdre C. Purfield, Donagh P. Berry, Stephen Conroy, Craig P. Murphy, Ross Evans

Department of Biological Sciences Publications

Genetic selection has been identified as a promising approach for reducing enteric methane (CH₄) emissions; a prerequisite for genetic evaluations; however, these are estimates of the necessary genetic parameters based on a population representative of where the genetic evaluations will be used. The objective of this study was, therefore, to derive genetic parameters for a series of definitions of CH₄, carbon dioxide (CO₂), and dry matter intake (DMI) as well as genetic correlations between CH₄, CO₂, and DMI in a bid to address the paucity of studies involving methane emissions …

A Reliable Diabetic Retinopathy Grading Via Transfer Learning And Ensemble Learning With Quadratic Weighted Kappa Metric, Sai Venkatesh Chilukoti, Liqun Shan, Vijay Srinivas Tida, Anthony S. Maida, Xiali Hei

Computer Science Faculty Publications

The most common eye infection in people with diabetes is diabetic retinopathy (DR). It might cause blurred vision or even total blindness. Therefore, it is essential to promote early detection to prevent or alleviate the impact of DR. However, due to the possibility that symptoms may not be noticeable in the early stages of DR, it is difficult for doctors to identify them. Therefore, numerous predictive models based on machine learning (ML) and deep learning (DL) have been developed to determine all stages of DR. However, existing DR classification models cannot classify every DR stage or use a computationally heavy …

Tumor-Associated Antigen Targets For Novel Immune-Based Strategies In Prostate Cancer, Amman Bhasin, Patrick Mille, Aditya Eturi, Andrew Iskander, William Tester, Kevin Zarrabi

Kimmel Cancer Center Faculty Papers

Prostate cancer remains the most common malignancy among men in the United States. Advancements in androgen receptor signaling blockade have led to landmark approvals for its use in patients with locally advanced and metastatic disease. However, additional novel therapeutic strategies for both hormone-sensitive and castration-resistant diseases remain ongoing areas of study. Thus, we turn to the growth of immuno-oncology, which has led to improved treatment outcomes for a variety of hematologic and solid tumor malignancies. Prostate cancers have shown only modest results with immune checkpoint inhibition in published trials, and innovative strategies are now looking into enhancing cytotoxic T-cell activity …

Impacts Of Harvest Date And Concurrent Alkali Pretreatment And Ensiling On Anaerobic Digestion Of Pennycress Biomass, Liangcheng Yang, Tuba Yasmin Lubna, Michael A. Moklak, Barsanti Gautum, Nicholas J. Heller, Robert L. Rhykerd, David E. Kopsell, John C. Sedbrook

Faculty Publications – Biological Sciences

Pennycress (Thlaspi arvense L.) is an annual cover crop known for its exceptional cold tolerance and high oil and protein yields. Pennycress can be integrated into a corn–soybean rotation in the U.S. However, the utilization of pennycress biomass remains largely unexplored, including assessing compositional changes through its growth and organic matter digestibility. This study harvested pennycress at three growth stages, characterized the biomass for anaerobic digestion (AD), and tested the effects of concurrent alkali pretreatment and ensiling on the biomass methane yield. Results showed that the biomass harvested when the plants were undergoing senescence (“third-harvest”) had higher contents of …

Dynamic Prognosis Prediction For Patients On Dapt After Drug-Eluting Stent Implantation: Model Development And Validation, Ahmed Abdelhameed, Xinyue Hu, Zenan Sun, David Aguilar, Abhijeet Dhoble, Jingcheng Du, Qing Wang, Shuteng Niu, Yifang Dang, Xinyuan Zhang, Ziqian Xie, Yi Nian, Jianping He, Yujia Zhou, Jianfu Li, Mattia Prosperi, Jiang Bian, Degui Zhi, Cui Tao

Faculty, Staff and Student Publications

BACKGROUND: The rapid evolution of artificial intelligence (AI) in conjunction with recent updates in dual antiplatelet therapy (DAPT) management guidelines emphasizes the necessity for innovative models to predict ischemic or bleeding events after drug-eluting stent implantation. Leveraging AI for dynamic prediction has the potential to revolutionize risk stratification and provide personalized decision support for DAPT management.

METHODS AND RESULTS: We developed and validated a new AI-based pipeline using retrospective data of drug-eluting stent-treated patients, sourced from the Cerner Health Facts data set (n=98 236) and Optum's de-identified Clinformatics Data Mart Database (n=9978). The 36 months following drug-eluting stent implantation were …

Land Use Change And Forest Management Effects On Soil Carbon Stocks In The Northeast U.S., Lucas E. Nave, Kendall Delyser, Grant M. Domke, Scott M. Holub, Maria K. Janowiak, Adrienne B. Keller, Matthew P. Peters, Kevin A. Solarik, Brian F. Walters, Christopher W. Swanston

Michigan Tech Publications

Background: In most regions and ecosystems, soils are the largest terrestrial carbon pool. Their potential vulnerability to climate and land use change, management, and other drivers, along with soils’ ability to mitigate climate change through carbon sequestration, makes them important to carbon balance and management. To date, most studies of soil carbon management have been based at either large or site-specific scales, resulting in either broad generalizations or narrow conclusions, respectively. Advancing the science and practice of soil carbon management requires scientific progress at intermediate scales. Here, we conducted the fifth in a series of ecoregional assessments of the effects …

Comparison Of Milk Microbiota Between Healthy And Mastitic Cows, Sibel Kizil, Fatma Esin Aydin, Cansu Önlen Güneri̇, Ufuk Ülker, Gürol Emekdaş, İbrahim Basmaci, Serkan Erat, Rıza Durmaz, Efsun Melike Çeçen, Asya Kazan

Turkish Journal of Veterinary & Animal Sciences

Mammary gland infections occur due to bacterial changes in the mammary tissue. Studies conducted in recent years have reported variations in the most common bacteria differ according to geographical locations. California mastitis test (CMT), somatic cell count (SCC), and aerobic colony count (ACC) analyses were performed on approximately 50 mL of hygienically collected raw milk samples. Raw milk was also subjected to conventional bacteriological isolation and identification. Bacterial diversity and rates in raw milk were compared through metagenome analysis. Two samples, one from healthy milk and another from subclinical milk with mastitis, were independently tested to determine whether there were …

Determination Of Antibiotic Resistance In Salmonella Typhimurium And Salmonella Kentucky Serotypes Of Animal Origin Using Conventional And Molecular Methods, Zeynep Şik, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

The high incidence of multidrug-resistant Salmonella Typhimurium and Salmonella Kentucky isolates is a concern for human and animal health. This study aimed to investigate the antibiotic resistance determinants of a total of 150 S. Typhimurium and S. Kentucky isolates obtained from cows, calves, lambs, and poultry. Salmonella isolates were tested against 13 different antimicrobials using the Kirby-Bauer disc diffusion method, and genotypic antimicrobial resistance determinants were investigated by polymerase chain reaction. Significant differences were detected among serovars for gentamicin, streptomycin, ampicillin, sulfonamide, nalidixic acid, ciprofloxacin, and tetracycline resistance, with the rates of resistance to these antibiotics being determined to be …