Life Sciences Commons^™

Breeding For Persistence In Lotus Corniculatus, M Rebuffo, N Altier

IGC Proceedings (1977-2023)

The objectives of this study were to develop a more persistent germplasm of birdsfoot trefoil (Lotus corniculatus L.) and to evaluate its potential use in Uruguay. Two cycles of phenotypic recurrent selection were done under field conditions. Eight hundred and 1600 spaced plants were established for the first and second cycle of selection. Both nurseries were visually assessed for crown rot, relative growth, plant habit and foliar diseases. The final selection of the breeding population LE 65-56 includes thirty six plants selected from sixteen elite families. This selection was more persistent and productive than the check varieties in plot …

Apomixis And The Reproductive Dynamics Of Eastern Gamagrass Tripsacum Dactyloides L., B Kindiger, C L. Dewald

IGC Proceedings (1977-2023)

Studies were conducted to determine the reproductive characteristics of Tripsacum, a perennial, native pasture species and to identify methods for breeding agronomically superior cultivars. Reproductive methods and types of individuals generated were evaluated with triploid and hexaploids possessing a novel method for transferring sexual germplasm into apomictic tetraploids and tetraploid germplasm into sexual diploids. Triploids and hexaploids are demonstrated to be useful materials for introgressing and transferring desirable alleles across ploidy levels and reproductive barriers.

Cytogenetical Studies In Range Grasses Of Iran, M Sheidai, A Tavasoli, P Derakhshandeh

IGC Proceedings (1977-2023)

Cytogenetical and breeding studies of range grasses are in hand. Present article describes preliminary cytogenetical analysis of wheatgrass (Agropyron) and bromegrass (Bromus) taxa (Agropyron trichophorum n = 21, A. pectinoforome n = 21), two different populations of A. repens (n = 28) and Bromus stenostachyus (n = 14). The species varied with regard to chiasma number and distribution. The highest value occurred in A. repens (Mako population). Heterogenity test for the paired samples showed lack of heterogenity for chiasmata indicating homogenity of Agropyron taxa. However a test for ring and rod bivalents showed significant differences …

Direct Selection Response For Stem Rust Resistance In Tall Fescue, R E. Barker, R E. Welty

IGC Proceedings (1977-2023)

Resistance to stem rust (caused by Puccinia graminis Pers.: Pers. subsp. graminicola Z. Urban.) would be beneficial in tall fescue (Festuca arundinacea Schreb.). Two cycles of polycross (PX) selection on progenies from thirty-four parent plants (14 forage-types and 20 turf-types) were compared to one cycle of open pollination (OP) followed by one cycle of PX selection using a 2-stage greenhouse screening process. Response from selection was determined from composite half-sib progenies from each cycle. Number of plants with resistant reaction based on pustule type increased from 5 to 54% in the PX forage-type population and from 6 to 50% …

Assessment Of Grazing Procedures In The Evaluation Of Plant Breeding Material, D Real, I L. Gordon, J Hodgson

IGC Proceedings (1977-2023)

Four stocking densities (2, 3, 5 and 9 sheep/18 m2 for one hour) and two times of grazing (morning or evening) were imposed on a spaced plant nursery of nine red clover (Trifolium pratense L.) populations of varying growth habit, in order to optimize measurement of sheep grazing preference. Plant height, spread and leafiness were measured before and after each of three grazings. Sheep preference was viewed multivariately, using combined measurements of these plant characteristics. MANOVA revealed that the effect of stocking density was significant (P-0.05), but that of time of grazing was not. The plant, population and overall …

Developing Well Adapted Early To Midseason Cultivars Of Trifoiloum Subterraneum Ssp. Brachycalycinum, C T. De Koning, P G.H. Nichols, R L. Tuckwell, N Schubert

IGC Proceedings (1977-2023)

Development and selection of early to midseason cultivars of subterranean clover (Trifolium subterraneum L.) ssp. brachycalycinum has mostly involved the use of early generation selection nursery swards (F2-progeny method). Four sites were sown in the mid-North region of South Australia. At the end of 1995, the top 20 lines from each site had burrs (seed pods) sampled at random. The burr will proceed into the final early generation selection phase (1 - 2 years). Dependent on the site, many lines have out-performed the standard checkplot cultivar, Clare. High hardseed levels have also been incorporated into 1989 crossbreds.

Regeneration Of Somatic Hybrids Between Festuca Arundinacea Var. Glacescens And Lolium Multiflorum Lam., T Sasaki, T Hasegawa, S Ueda

IGC Proceedings (1977-2023)

Protoplasts derived from ten suspension cultures of tetraploid Festuca arundinacea var. glaucescens (2n = 4x = 28) and diploid Lolium multiflorum Lam. (2n = 2x =14) were fused to produce somatic hybrids. Regenerated green plants in three fusion combinations were identified as somatic hybrids because they showed characters that were peculiar to both Festuca arundinacea var. glaucescens (hairs on auricle) and Lolium multiflorum Lam. (root-fluorescence). Moreover, the hybrids also had a chromosome number of about forty two.

Breeding Morphogenetic Traits To Match Genotypes To Their Utilization, L Hazard, M Betin, M Ghesquiere

IGC Proceedings (1977-2023)

A divergent selection for lamina length was done from natural populations of perennial ryegrass. Tested in a multi-site experiment in France, the short-leaved perennial ryegrass was more productive under simulated grazing and less productive under infrequent cutting than the long-leaved perennial ryegrass. Matching cultivars to their management is possible by selecting for morphogenetic traits. However, our results suggest the range of adaptation to different managements of perennial ryegrass cultivars could be extended by increasing their phenotypic plasticity.

Improving Forage Productivity By Using Cytoplasmic Male Sterility Of Pearl Millet, A A. Younis, A H. Belal

IGC Proceedings (1977-2023)

Fourteen forage millet hybrids were produced by crossing 7 male fertile lines to 2 different lines of dwarf cytoplasmic male sterile (cms) lines of forage millet (Pennisetum americanum). Hybrids were evaluated with their male parents at the Giza experimental station in summer 1994. The evaluations included fodder yield traits and chemical composition. The objectives of the study were: 1 - to assess hybrid vigor, and 2 - to select the superior hybrids to be used in commercial production in Egypt. Present results showed that the 14 F1 hybrids and their male parents varied significantly in green and dry …

Protogynous Interval In Elephantgrass (Pennisetum Purpureum Schum.), A V. Pereira, R B. Barra, J C. Abreu, V De P. Freitas

IGC Proceedings (1977-2023)

A study was carried out to examine the protogyny duration in elephantgrass inflorescences. Nine accessions were selected, divided into three groups, according to early, intermediate or late flowering. From each cultivar, 10 panicles were sampled for evaluation. The criterion for estimating the duration of protogyny was the time interval (number of days) from the first visual detection of exserted stigmas up to the first visual appearance of open anthers (±5%). Duration of protogyny averaged 7.43±1.37 d, confirming that the species is protoginous. Late flowering accessions exhibited an average of 8.97 d for the duration of protogyny, which was significantly higher …

Evolution In Segregating Genotype Mixtures Of Subterranean Clover, P.G. H. Nichols, P S. Cocks

IGC Proceedings (1977-2023)

A complex segregating mixture of subterranean clover (Trifolium subterraneum L.), was sown in 1978 at Nabawa and Mt Barker, two contrasting sites in Western Australia. Seed harvested from both sites from 1980-1994 was grown out at Shenton Park in 1995, along with seed of the original mixture. Populations diverged markedly for mean days to first flowering (DFF) within three seasons. No further trends in mean DFF occurred in plants derived from the next 14 years, although standard deviation declined significantly in plants from Mt Barker. Between season differences in mean DFF were correlated with estimated growing season length at …

Prediction Of Seed Yield Of Festuca X Lolium Hybrids From The Nursery Mother Plants, C Mousset, L Hazard, M Ghesquiere

IGC Proceedings (1977-2023)

In order to develop commercial Festuca x Lolium hybrids, their seed production has to be improved. Seed yields of Festuca x Lolium progenies were assessed in dense plots. Prediction of those seed yields was based on the morphological observations done on spaced plants in the nursery of both mother plants and their progenies. The best prediction of the progeny seed yields is a linear regression on the stem density and the seed weight per inflorescence assessed on the mother plants.

Use Of Flow Cytometer In Breeding Festuca X Lolium Hybrids, M C. Kerlan, M Ghesquiere

IGC Proceedings (1977-2023)

Ploidy in various generic Lolium - Festuca progenies were assessed by flow cytometry and compared to conventional chromosome counting. In non-segregating triploid and tetraploid progenies, the cytometer estimated so accurately the level of plant ploidy that chromosomes counting would be no use. In segregant progenies, all the tetraploid plants according to the cytometer had effectively 28 chromosomes. Among the triploid and pentaploid plants detected by the cytometer, 40% of the plants were tetraploid. They represented only 29% of the progeny which must be counted. The flow cytometry can be used in routine interspecific breeding programmes. Conventional chromosome counting should be …

Persistency In Lolium X Festuca Hybrid Derivatives And Its Relationships With Flowering Traits, M Ghesquiere, C Mousset

IGC Proceedings (1977-2023)

Using amphiploid and backcrossed derivatives of Italian ryegrass x tall fescue hybrids, the paper emphasizes the interest of introgression vs amphiploidization in breeding for specific traits such as persistency or seed production from tall fescue or ryegrass resp. Persistency in amphiploid and Lolium-introgressed progenies were assessed in nursery together with variation of flowering traits. Persistency was found lower, on average, in introgressed progenies than in hybrid progenies but with enlarged variability within progeny suggesting possible advantageous rearrangements of fescue chromosomes. Although significantly associated, persistency in both populations was only very little affected by the variations in flowering traits such …

Field Evaluation Of Crown Rust In Annual Ryegrass Populations, G M. Prine

IGC Proceedings (1977-2023)

Crown rust (Puccinia coronata [Pers.] Cda.) is the most prevalent disease found on annual ryegrass (Lolium multiflorum L.) of the southeastern USA. Determination of crown rust resistance is difficult because of the erratic nature of infections at most locations. As crown rust is present at Gainesville, FL each year, a rust nursery was designed to evaluate rust resistance and susceptibility of annual ryegrass. Evaluation consists of periodic rust ratings of the seed crop and regrowth of part of each plot after cutting. Nursery results indicate the buildup of rust, including at the date of 90% heading (start of …

Selection Of Interspecific Hybrids Of Brachiaria-A Tropical Forage Grass, C B. Do Valle

IGC Proceedings (1977-2023)

The aim of the breeding program at the National Beef Cattle Research Center (CNPGC) of the Brazilian Corporation for Agricultural Research (EMBRAPA), is to produce vigorous apomictic hybrids with wide edaphic adaptation, good production and nutritive value combined with spittlebug (Homoptera:Cercopidea) resistance. Fiftysix superior hybrids were selected based on visual estimates of vigor, leafiness, tolerance to drought and natural spittlebug infestation in the field. These were characterized for mode of reproduction, spittlebug reaction under controlled conditions and for morphological diversity using nine selected traits. Multivariate analysis classified the population into seven groups where the hybrids were clearly intermediate in plant …

Mitochondrial Genome Polymorphism In Lolium Perenne, Y Shimamoto, Y Tominaga, M Sato, T Mikami

IGC Proceedings (1977-2023)

The restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) of perennial ryegrass (Lolium perenne L.) were investigated to elucidate the genetic relatedness among the 128 cultivars including diploid and tetraploid. Many patterns of RFLPs were observed and allowed assigning of the cultivars into the main eight haplotypes of mitochondrial genome relatedness. The American cultivars were classified into haplotype I and VIII which were remote at the mitochondrial genome from each other, the European ones were distributed to all haplotypes and the tetraploid ones were mostly assigned into the haplotype V. The assessment of mtDNA RFLPs may be a …

Inbreeding Of Some Populations In The Genus Agrostis L., Zorica Tomic, Gordana Surlan Momirovic, Dusica Delic, S Ostojic

IGC Proceedings (1977-2023)

This work presents some properties on produced inbred lines of the 5 best populations of 4 species of Agrostis for breeding purposes. The cultivars of forage grasses should meet a given aim for selection, which is the increasing dry matter and quality. The populations used in this research were the best ones for morphological and productive properties from a previous study. By the process of selfing there have been produced S1 offsprings which appeared to be quite superior in relation to open pollinated offspring from the mother plants. The obtained results were processed by multiple range regression analyses. According to …

The Effect Of Abscisic Acid On The Freezing Tolerance In Lolium Temulentum L., K Tase, H Fujii

IGC Proceedings (1977-2023)

This study was conducted to clarify the effect of abscisic acid (ABA) on the development of freezing tolerance in Lolium temulentum L. We compared the changes of freezing tolerance and endogenous ABA content by the ABA treatment with those by the hardening treatment. Exogenous ABA applications (7.5 x 10-5 M) increased freezing tolerance, but did not completely substitute for the hardening process. This treatment resulted in great increase in endogenous ABA content, as compared with the control or the hardening treatment. Since the relationship between endogenous ABA content and freezing tolerance was not parallel, it is considered that freezing tolerance …

Assessing The Reliability, Internal Consistency, And Sensitivity Of A Nutrition Knowledge Questionnaire For Four-Year-Old Pre-K Children, Lin Wang, Karla Cortez, Brenda Rodriguez, Joseph Reyes, Moises Cisneros, Elizabeth Alanis, Zasha Romero, Lisa Michelle Belzer Salinas, Juan López Alvarenga, Roberto Treviño-Peña

Research Symposium

Introduction: Assessing nutrition knowledge in four-year-olds, a developmental stage marked by limited attention spans and varying comprehension abilities, is challenging with traditional methods. A reliable test is pivotal for establishing a foundation for future health interventions. We evaluated a 13-question nutrition and healthy habits test in Pre-K4 children to assess reliability and sensitivity to detect differences.

Methods: Calculations included Cronbach's alpha, kappa coefficient, McNemar analysis by item, and Bland-Altman plots for test-retest differences. Mixed model regression assessed the questionnaire's sensitivity by sex and association with age. Item response theory (IRT) models were employed, generating latent abilities for students and individual …

Evaluation Of Ergosterol And Its Metabolites As Lxr Agonists And Their Anticancer Potential In Colon Cancer, Yogain Taank, Navneet Agnihotri

Research Symposium

Purpose: Aberrant cholesterol homeostasis is a well-recognized hallmark of cancer and implicated in metastasis and chemotherapeutic resistance, the two major causes of cancer associated mortality. Liver X receptors (LXRs) are the key transcription factors that induce cholesterol efflux via enhancing the expression of ABCA1 and ABCG1.

Methods: Molecular docking and dynamic simulation studies were done to assess the binding affinity and stability of the receptor ligand complexes. Activation of LXRs was evaluated using the luciferase reporter assay. qRT-PCR and western blotting was done to analyse the mRNA and protein expression of cholesterol homeostasis genes. Flow cytometric analysis was carried out …

The Degree To Which Secondary School Students Within The Green Line Possess Social Emotional Learning Competencies From Their Point Of View, أمل عواودة

Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات

The study aimed to identify the degree of high school students within the Green Line possessing social emotional learning competencies from their point of view. In addition to knowing the effect of variables (gender, school type) on students' estimates of that. The descriptive approach was used to achieve the purpose of the study, as the study sample consisted of (940) male and female students, who were selected in a simple random way from the study population of (19990) male and female students, and the questionnaire was used as a tool for data collection. The results of the study showed that …

Applicability Of Fibrous Cellulose –D In Extracting Double-Stranded Rna (Dsrna) Of Tobrfv, Raed Alkow, Reem Hijaz, Beesan Jamoos, Najah Khandaqji, We’Am Daraghmeh, Saja Salah, Alaa Bari, Rahaf Ben Ali, Osama Alabdalla

Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات

The viral replicative form double strand RNA (dsRNA) is a unique feature to the plant RNA viruses that used for non-specific detection of plant viruses. For decades; fibrous cellulose -11 (CF11) was used for dsRNA purification, but recently was no-more produced. Other fibrous celluloses (CF-A; -B; -C; -D; and –E) were suggested as alternatives to CF11. This study was to evaluate the applicability and reproducibility of using CF-D with two different dsRNA extracting methods: micro-spin column and modified Dodds extraction methods. The best results were recorded on Dodds extraction method over micro-spin one. This results also confirmed the applicability of …

Anti- Transpirants Impact On Pepper Cultivated Under Different Drought Stress Levels, Ola Alnaddaf, Elien Abo Saeb, Ayman Abo Assi

Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات

Drought is one of the most limiting factors for agricultural productivity worldwide (Hamdi et al., 2020). Drought stress can be simply defined as a shortage of water that causes significant changes in plant morphology, physiology, and biochemistry (Liang et al., 2020), and the most environmental stress that affect both crop production and quality (Biareh et al., 2022). Approximately, 95-98% of the water absorbed by the plant is lost via transpiration (Singh et al., 2021), so that reducing the transpiration rate could be considered as an important strategy for combating with drought conditions. This could be achieved by using compounds called …

Effect Of Adding Different Levels Of Oregano Leaves Extract (Origanum Vulgare) In Drinking Water On The Quality Characteristics Of The Carcass Of Broiler Chickens, Nihad Ali, Motasem Al Massad

Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات

The field Animal Production Department (College of Agriculture / Al-Qasim Green University) was chosen to conduct the project from 18/9/2019 to 23/10/2019. In it 240 chicks of Ross 308 were used. The chicks were randomly divided into four experimental trials. The leaves extracted suspension was added at 0, 3, 5 and 7 ml/liter to drinking water, and the following characteristics were studied: live body weight before slaughter and the weight of the carcass , dressing rate, the relative weights of the main and secondary carcass parts, and the relative weights of the eating giblets.

The results showed that the fourth …

Microclimate, An Important Part Of Ecology And Biogeography, Julia Kemppinen, Jonas J. Lembrechts

School of Biological Sciences: Faculty Publications

Brief introduction: What are microclimates and why are they important? Microclimate science has developed into a global discipline. Microclimate science is increasingly used to understand and mitigate climate and biodiversity shifts. Here, we provide an overview of the current status of microclimate ecology and biogeography in terrestrial ecosystems, and where this field is heading next.

Microclimate investigations in ecology and biogeography: We highlight the latest research on interactions between microclimates and organisms, including how microclimates influence individuals, and through them populations, communities and entire ecosystems and their processes. We also briefly discuss recent research on how organisms shape microclimates from …

Description Of A New Species (Eucypris Dorlionensis N. Sp.) (Ostracoda, Crustacea)From Türkiye And A Cladistic Analysis Of The Genus Eucypris, Fi̇li̇z Batmaz, Mehmet Yavuzatmaca, Okan Külköylüoğlu

Turkish Journal of Zoology

This is the first clustering analysis of the 53 species of the genus Eucypris where we used 28 plesiomorphic and apomorphic character states in the Winclada/NONA program. Accordingly, Eucypris dorlionensis n. sp. was clustered within Group 2, in which the new species was clearly separated from the other species of the genus based on the shape of the carapace, presence of fine hair-like setae on the carapace surface, the position of the maximum height of the carapace, and length ratio of terminal claws of the second antenna. Eucypris dorlionensis n. sp. was collected from a trough in Eskişehir province in …

Envisioning Transition From Open Landscapes To Forested Landscapes In The Routt National Forest, Colorado, United States, Brice B. Hanberry, Jacob M. Seidel

Aspen Bibliography

Globally, in remaining wildlands, tree densities and forested cover have increased in grasslands and open forests since European settlement. In the southern Rocky Mountains of Colorado, United States, we determined tree composition and tree cover from historical (years 1875 to 1896) surveys and compared them to current (2002 to 2011) tree composition and current (year 2016) forested land cover for 500,000 ha of the Routt National Forest. Additionally, we examined whether changes in precipitation occurred. Regarding composition, pine (primarily lodgepole pine; Pinus contorta) decreased from 65% to 32% of all trees, with increased subalpine fir (Abies lasiocarpa) …

When And Why Ecological Systems Respond To The Rate Rather Than The Magnitude Of Environmental Changes, Karen C. Abbott

Faculty Scholarship

Ecologists and conservation biologists have become quite familiar with the concept of tipping points: abrupt changes in an ecosystem's state that occur after a period of relative stasis. Most of the familiar ecological examples of tipping points occur either because a once-stable state has lost stability, or the system has been subjected to a particularly large perturbation and transitions to an alternative stable state, distinct from the pre-perturbed state. A different class of tipping points, known as rate-induced tipping (or r-tipping) points, are likely present in many ecological communities but remain little known in the field. Rate-induced tipping occurs when …

Catatonia As A Result Of A Traumatic Brain Injury, Jessica Berthelot, Jacob Cambre, Madeline Erwin, Jennifer Phan

School of Medicine Faculty Publications

Catatonia is a neuropsychiatric syndrome typically marked by disturbances in motor activity, speech, and behavior. It has historically been associated with psychiatric illness, but acute medical illness, neurocognitive disorders, and neurodevelopmental disorders can cause catatonia as well. Catatonia is likely underrecognized and underdiagnosed in the general medical hospital, despite high risks of morbidity and mortality and the availability of rapidly effective treatment. Here, we present a case of catatonia secondary to traumatic brain injury that responded to lorazepam after a delayed diagnosis. A young male patient who was incarcerated and assaulted was sent to the emergency department multiple times for …