Life Sciences Commons^™

Early Ontogenetic Responses Of Six Commercial Chloris Gayana Cultivars To Salinity, E Taleisnik, H Perez, G Peyrano, C Arias

IGC Proceedings (1977-2023)

Some physiological aspects relevant to pasture establishment under saline conditions were evaluated in six cultivars of Chloris gayana . Two tetraploid cultivars: Boma and Callide, and four diploid ones: Bell, Katambora, Pioneer and a local accession of Pioneer, were analyzed. The effect of salinity on seedling emergence, the number of stolons per plant and frost tolerance were evaluated in the field, while effects on germination, early vegetative growth, regrowth after clipping, ion accumulation and stolon rooting were assessed in the greenhouse. Salinity had a negative effect on seedling emergence. Saline solutions delayed or altogether inhibited germination, but seeds retained viability …

Interpreting Indicators Of A Transfer Of N From Legume To Grass In Competition Studies, P G. Tow

IGC Proceedings (1977-2023)

The transfer of N from legume to grass in mixtures is important for regulating competition. In competition studies which include both monocultures and mixtures, N transfer should be detectable by grass N measurements but may be masked by competitive reductions in grass growth. In the study reported here, whole plant DM and N were measured in Panicum maximum var trichoglume and Neonotonia wightii grown in monocultures and additively in mixtures. While competition reduced whole grass plant DM and N in mixture relative to monoculture, higher grass shoot % N and N yield in the mixture at early harvests appeared reliable …

A Comparison Of Production And Soils In Modified Mixed Prairie Community, S W. Porter, W D. Willms, J F. Dormaar, B H. Ellert, H H. Janzen

IGC Proceedings (1977-2023)

This study examined the effects of reseeding a Stipa-Agropyron- Bouteloua community to monocultures of crested wheatgrass (Agropyron cristatum (L.) Geartn.), Russian wild rye grass (Elymus junceus Fisch.), and wheat (Triticum aestivum L.) on above and below ground biomass and indicators of soil quality. Summer fallowed wheat produced 10817 kg ha-1 and 4090 kg ha-1 of above ground below ground biomass respectively whereas the native community produced 3191 kg ha-1 above ground and 13013 kg ha-1 of below ground biomass. However wheat, crested wheatgrass and Russian wildrye grass generally showed a lower biological index, phosphatase and dehydrogenase activity.

Nitrogen Use Efficiency Of Timothy Populations, G Belanger, C Murray, R Michaud, F Gastal, J E. Richards

IGC Proceedings (1977-2023)

The objective of this study was to determine the variability in N use efficiency among field-grown timothy (Phelum pratense L.) populations. Shoot biomass and N uptake were measured at the end of the spring growth cycle on six timothy populations fertilized with three N rates at two sites in Eastern Canada. The variability in shoot biomass among populations was similar under limiting and nonlimiting N conditions. The ranking of the populations, however, differed under limiting and non-limiting N conditions, and also between the two sites under limiting N conditions. The differences in shoot biomass among populations under highly N …

Characterization Of The Leaf Anatomy Of Panicum Virgatum Germplasm, D S. Fisher, J C. Burns, J P. Pienaar

IGC Proceedings (1977-2023)

Both the yield and digestibility of C4 grasses have been related to anatomical characteristics. The objectives of the current work were to test switchgrass with varied yield and digestibility for variation in anatomical measurements and to develop a method of graphically representing the anatomy. Six accessions were established in replicated field plots and the lamina were sampled, dehydrated and embedded for anatomical study. Midrib dimensions were excluded from study but perimeters of primary and secondary vascular bundles in the lamina were measured. Lamina thickness was determined at the primary bundle, secondary bundle and at the minimum between the bundles. The …

Relation Of Root And Shoot Morphology Of Grass Seedlings, L E. Moser, A J. Smart

IGC Proceedings (1977-2023)

Grass seedling establishment is dependent on adventitious root development. However, evaluating the establishment status of a seeding by excavating a population of seedlings and determining root morphology is difficult and generally will not be undertaken. Based on both field and greenhouse studies we have found that root and shoot morphological development is closely related within species, for intermediate wheatgrass, [Thinopyrum intermedium (Host) Barkw. and D.R. Dewey], smooth bromegrass (Bromus inermis Leyss.), switchgrass (Panicum virgatum L.), and big bluestem (Andropogon gerardii Vitman) seedling populations. Easily observable developmental stages of seedling shoots were related to adventitious root development. …

Effect Of Weekly And Triweekly Application Of Growth Hormone Gibberellin On Dwarf Millet Plants, Holly Harding, Kendall Klewer, Delanee Miller, Therie Moore, Clara Gallapoo

2024 Student Academic Showcase

Plant biology students teamed up with statistics students to examine the effects of the plant growth hormone gibberellin on mutant millet plants. Dwarf mutant millets exhibit stunted growth either because they do not produce gibberellin on their own, or because they lack the mechanisms to effectively use gibberellin. By growing some of these mutants in the greenhouse, applying a gibberellin solution to them, and observing their growth response, we can identify which kind of mutant the plant is. We are also examining the importance of frequency of gibberellin application, with some plants receiving one application of gibberellin per week, and …

Mechanisms Of Genetic Dwarfism In Dwarf Millet Genotypes, Holly Willett, Savannah Jones, Rashmi Aryal, Therese West, Maddelyne Wright

2024 Student Academic Showcase

The goal of this project was to study and determine the different forms of genetic dwarfism in millet plants. This was done by creating three different types of millet samples using two different forms of mutant seeds and also normal seeds. 6 plants of each of the three varieties were sectioned into separate pots, and then given different treatment levels of gibberellin. Two plants in each group were given no gibberellin, two were given gibberellin once a week and two were given gibberellin daily Monday-Friday. The height of each plant was then recorded once a week throughout the trial period. …

Longitudinal Dynamic Strength Index Profiling In Collegiate Olympic Weightlifters Preparing For National Competition, Jose Mostaffa

2024 Student Academic Showcase

PURPOSE: Characterize DSI scores and evaluate longitudinal changes in DSI and its constituent variables in collegiate Olympic weightlifters.

METHODS: Male and female weightlifters performed three trials of CMVJ and IMTP once per week during the final eight-week peaking period prior to competition. Mixed effects models were computed to identify week-to-week differences in DSI, CMVJ, and IMTP performance (α = 0.05). Cohen’s d effect sizes were calculated comparing between each week and baseline.

RESULTS: Significant changes in CMVJ peak propulsive force, IMTP peak force, and DSI were not detected throughout the testing period. Effect sizes were small for …

Pilot Project: Plant Hormones And Soil Bacterial Populations In Plant Growth Enhancement, Ada Sardana Sambola

2024 Student Academic Showcase

The way microorganisms affect plant health, through the contribution of nitrogen or phosphorus fixation by bacteria to the growing plants, has been thoroughly studied. Commonly referred to as Plant Growth Promoting Bacteria (PGPBs), these organisms play a key role in enhancing plant growth by assisting in the production of indole acetic acid (IAA), facilitating the uptake of nutrients via mechanisms like phosphorus solubilization, and producing inhibitory substances that protect plants from pathogens. However, the reverse of this phenomenon has not been studied as deeply. This study will focus on how the addition of plant hormones, more specifically, gibberellins, affects the …

Gibberellic Acid Influence On Mutant Millets: An Exploration Of Effects, Fokam Felix Thierry Foading, Kennedy Femmer, Blake Reiger, Matthew Turray

2024 Student Academic Showcase

Setaria viridis, commonly known as green foxtail millets, exhibits diverse physical traits, including instances of dwarfism. Our study investigated the effect of gibberellic acid on millets, focusing on growth responses in normal and mutant plants, including dwarf variants. Gibberellic acid plays a vital role in stem elongation, and its deficiency may lead to hindered growth. We conducted experiments applying a gibberellic acid concentration of 0.5g/L to determine its impact on plant height. Two mutant types, Setaria Viridis 11970 Mutant 4 and Setaria viridis 03054 Mutant 6, were studied alongside the normal millet type Setaria Viridis A10.1 wild type. Results showed …

Ty4 Retrotransposon Presence In Yeast Strains, Maria P. Walters, Eric T. Gillock

SACAD: Scholarly Activities

Yeast is used in everyday life. It is important to understand the genetic components of yeast. In various laboratory yeast strains, there are findings of TY1, TY2, and TY3. TY1 through TY3 have been well studied, while TY4 and TY5 are retroelements that have yet to be thoroughly studied. It is known that TY4 is found at only one to three copies per haploid genome.

TY4 is a retrotransposon, which shares similarities with retroviruses, making it essential to study its presence in

yeast. This study aims to evaluate the prevalence of TY4 among lab strains, brewing strains, and baking strains …

Using Artificial (Ai) To Predict A Structure Of Protein Complex, Yiqing Zang

SACAD: Scholarly Activities

Proteins play pivotal roles in essential life processes and elucidating their three-dimensional (3D) structures is crucial for understanding their functions. AlphaFold2, an advanced artificial intelligence-based method developed by Google DeepMind, has emerged as a promising tool for predicting protein structures. In this study, we evaluated the predictive capabilities of AlphaFold2. Our findings highlight AlphaFold2's efficacy in providing valuable insights into protein structure prediction, albeit with certain limitations. While AlphaFold2 represents a significant advancement in the field, its utility is best realized when integrated with complementary experimental approaches. Consequently, combining the strengths of AlphaFold2 with experimental validation remains essential for achieving …

Anything But Another Essay: Understanding Undergraduate Stem Student Perceptions Of Creative, Project-Based Learning, Emily M. Redding, Lorelei E. Patrick

SACAD: Scholarly Activities

The training of innovative and diverse scientists starts with creative teaching practices. Much of the pedagogy literature on incorporating nontraditional teaching practices in college STEM courses focuses on the use of in-class active learning activities. Less study has been devoted to creative, project-based assignments, such as “UnEssays”. The “UnEssay” is a semester-long assignment that challenges students to complete a library research project on a topic of their choosing and present it using anything but an essay. Although gaining popularity, few studies have assessed STEM student's perceptions of the assignments. To do so, Zoology students were asked to complete pre- and …

Screening Environmental Soil Samples For Antibiotic Production, Paige Mattick

SACAD: Scholarly Activities

Antibiotic resistance is a detrimental worldwide challenge, producing bacterial infections that are progressively more difficult to treat and cure. To attempt to help alleviate this issue, we screened soil samples for the presence of antibiotic-producing microorganisms. Soil samples were collected and diluted to 1:100 and 1:1000 ratios of soil and distilled water. These soil mixtures were then streaked onto tryptic soy agar (TSA) plates and incubated at 30℃ until colonies developed. These colonies were then selected and plated on a lawn of Serratia marcescens, which was utilized as the target organism.

Serratia marcescens was selected due to its known resistance …

A Novel Study Of Cliff Swallow (Petrochelidon Pyrrhonota) Feather Coloration In Relation To Habitat Characteristics, Colony Size, And Body Condition, Sonja Brandt, Medhavi Ambardar

SACAD: Scholarly Activities

Feather coloration is used for social signaling in many avian species, and can be associated with their ability to live and breed in habitats with high quality resources (Jenkins et al. 2013, Saino et al. 2013). It can signify individual quality, (Saino et al. 2013) and influence mate choice (Bennet et al. 1996). We analyzed different aspects of Cliff Swallow (Petrochelidon pyrrhonota) feather coloration in relation to morphology and habitat characteristics. We measured luminance, hue (theta and phi), and saturation for four different color patches on the swallows. We predicted that individuals in brighter coloration would be able to settle …

Presence Of Porcine Endogenous Retrovirus C In Domestic Pigs In Kansas, Avery Welton, Eric T. Gillock

SACAD: Scholarly Activities

There is currently a shortage of organs available to perform allotransplants in humans, prompting the exploration of xenotransplantation as an alternative. Xenotransplantation is the transplantation of living tissues between different species, with porcine tissues being a promising option. However, concerns arise regarding cross-species transmission, particularly in relation to Porcine Endogenous Retroviruses (PERVs). PERVs are retroviruses that are integrated into the germ-line cells of pigs and are permanently embedded in the host cell’s DNA, allowing for vertical transmission. Of significant concern is the infectious nature of two PERV subtypes, A and B, to humans, which can potentially recombine with the third …

The Conceptual Foundations Of Innate Immunity: Taking Stock 30 Years Later, Thomas Pradeu, Bart P.H.J. Thomma, Stephen E. Girardin, Bruno Lemaitre

Presidential Fellows Articles and Research

While largely neglected over decades during which adaptive immunity captured most of the attention, innate immune mechanisms have now become central to our understanding of immunology. Innate immunity provides the first barrier to infection in vertebrates, and it is the sole mechanism of host defense in invertebrates and plants. Innate immunity also plays a critical role in maintaining homeostasis, shaping the microbiota, and in disease contexts such as cancer, neurodegeneration, metabolic syndromes, and aging. The emergence of the field of innate immunity has led to an expanded view of the immune system, which is no longer restricted to vertebrates and …

Native Wetland Plant Seed Collection And Cleaning Guide For The Intermountain West, Rae Robinson, Maddie Houde, Elana Feldman, Samantha R. Kurkowski, Nathan M. Crawford, Stephanie Aristizabal, Karin M. Kettenring

All Current Publications

Collecting and cleaning seeds from wildlands is an essential part of ecosystem restoration, management, and conservation. Here, we summarize best practices for native wetland plant seed collection and cleaning in the Intermountain West.

A companion guide on seed testing, "Seed Viability Testing Guide for Common Wetland Plant Species," is also available (https://digitalcommons.usu.edu/extension_curall/2389/).

"The Relevant History And Medical And Ethical Future Viability Of Xenotransplantation", Morgan Janes

Augustana Center for the Study of Ethics Essay Contest

Xenotransplantation, the transplantation of organs or tissues from one species to another, presents a complex nexus of medical, ethical, and cultural considerations. In this article, we delve into the multifaceted landscape of xenotransplantation, beginning with a thorough examination of its relevant historical trajectory. From early experiments to recent advancements, we chart the evolution of this field, setting the stage for a nuanced discussion. We then confront the central issue: the true medical viability of xenotransplantation and the looming specter of operative risk. By scrutinizing the ethical dilemmas inherent in xenotransplantation through a multicultural lens, we illuminate the diverse perspectives that …

Time Spent Jogging/Running And Biological Aging In 4,458 U.S. Adults: An Nhanes Investigation, Christina Michelle Blackmon

Theses and Dissertations

Telomere length is a good index of cellular aging. Longer telomeres are predictive of longer life, and healthy lifestyles are associated with longer telomeres. This study explored the relationship between time spent jogging or running each week and leukocyte telomere length (LTL) in 4,458 randomly selected U.S. adults. The association was studied using data collected by the National Health and Nutrition Examination Survey (NHANES), and a cross-sectional design. Total weekly jog/run time was calculated from survey responses. From the minute totals, three categories were formed: <10 minutes/week, 10-74 minutes/week, and ≥75 minutes/week. Adults in the third category met the U.S. guidelines. Data were analyzed using one-way ANOVA. Partial correlation was used to adjust for differences in potential mediating factors, including demographic and lifestyle/medical factors. In the total sample, after adjusting for all the potential covariates, mean LTL significantly differed across the 3 jog/run categories (F=4.1, P=0.0272). Specifically, adults who met the guidelines via jogging and/or running had significantly longer telomeres than adults who performed no jogging/running. Adults in the middle category did not differ from the other two categories. A minimum of 75 minutes of jogging/running weekly is predictive of longer telomeres when compared to adults who do not jog or run regularly.

Effects Of Gibberellin On Stem Elongation, Annamarie Busch, Bryce Mcquerrey, Guillermo Bilbao Olarreaga, Duncan Krige, Eduan Van Herrden

2024 Student Academic Showcase

Plant growth hormones are widely used in the agricultural industry to manipulate the growth of crops. Dwarf mutant variants of plants have been found to have a variety of mutations that inhibit the synthesis and metabolism of gibberellin, a major plant growth hormone (Phinney 1956). Observing the effect that the application of gibberellin has on the growth in 2 types of dwarf mutant millet variants will allow us to determine the type of mutation present. Applying a gibberellin solution is expected to increase the rate of stem elongation in variants with the mutation that inhibits the production of gibberellins. If …

Growth In Response To Gibberellin In Setaria Viridis Mutants, Jayla Hampton, Rain Hongsermeier-Baxter, Joseph E. Smith, Seth Baur, Brooke Gawlick

2024 Student Academic Showcase

Gibberellin is a plant hormone that promotes growth in plant organs. Issues arising with the production and process of the hormone result in the mutation dwarfism. In this experimentation, the effects of gibberellin on dwarf Setaria viridis are isolated by controlled growth environments and treatments. The effects of the hormone treatment are measured manually over the period of growth so that the data may be analyzed and determine the cause of dwarfism in our species. In conclusion, this study offers important insights into the mechanism underlying dwarfism mutation, providing potential direction for future research and understanding plant physiology.

Research Proposal: Examination Of Bacterial Communities In Soil Beneath Decaying Organic Matter And The Effect Of Insect Succession, Olivia Fotiadis

2024 Student Academic Showcase

Bacterial species of the Proteobacteria, Acidobacteria, and Bacteroidetes phyla are key components in decomposing organic matter by secreting specialized enzymes into decaying material, facilitating its breakdown and nutrient release. Diverse microbial communities in areas of decomposition are known for their efficiency in decomposition and nutrient cycling. Less is understood about the effect of insects introducing unique microbes alongside initial soil bacteria, like Bacillus subtilis, in the decomposition process. Studying the decomposition of pork samples as a human analog will explore variations in bacterial populations in soil before and after the introduction of insect-mediated microbes and provide information on the efficiency …

Unraveling Gibberellic Acid's Impact On Plant Growth: Insights From Dwarf Millet Varieties, Brandy Miskell, Jacob Campagna, Alyssa Lambert, Dean Palmer, Bruno Ninez

2024 Student Academic Showcase

Gibberellic acid (GA), derived from the Bakanae fungus Gibberella fujikuroi, acts as a plant hormone with various functions. This study compares the growth of Setaria viridis (millet) wild type with two dwarf mutant varieties (mutant 11970 and mutant 03054) to understand genetic dwarfism and GA's role. Thirty seeds of each variety were planted, with biweekly GA treatments given to four pots and two serving as controls. Results show significant differences in leaf growth, indicating GA's role in regulating plant height. However, the exact mechanisms of GA transport within plants remain unclear, warranting further research to understand its implications for plant …

The Changes In Oral Flora After Oral Hygiene, Brooklyn Bumbales

2024 Student Academic Showcase

The goal of this project is to study the changes in the oral flora of an individual's mouth pre-cleaning and post-cleaning due to everyday brushing. The oral flora comprises many microorganisms such as Staphylococcus and Lactobacillus that are constantly changing. In previous studies, the diversity and abundance of these microorganisms in the oral cavity are subject to change in the times following pre-cleaning and post-cleaning. This study will examine saliva samples from consenting individuals, will be collected pre-cleaning (brushing) and post-cleaning of the oral cavity. The samples will then be tested for the population size and diversity of different microorganisms …

Nanocavity-Mediated Purcell Enhancement Of Er In Tio2 Thin Films Grown Via Atomic Layer Deposition, Muchuan Hua, Jianguo Wen, Sagar Kumar Seth, Connor P Horn, Ignas Masiulionis, Manish Kumar Singh, Sean E Sullivan, F Joseph Heremans, David D Awschalom, Supratik Guha, Alan M Dibos

Faculty, Staff and Student Publications

The use of trivalent erbium (Er3+), typically embedded as an atomic defect in the solid-state, has widespread adoption as a dopant in telecommunication devices and shows promise as a spin-based quantum memory for quantum communication. In particular, its natural telecom C-band optical transition and spin-photon interface make it an ideal candidate for integration into existing optical fiber networks without the need for quantum frequency conversion. However, successful scaling requires a host material with few intrinsic nuclear spins, compatibility with semiconductor foundry processes, and straightforward integration with silicon photonics. Here, we present Er-doped titanium dioxide (TiO2) thin film growth on silicon …

Increased Dietary Trp, Thr, And Met Supplementation Improves Performance, Health, And Protein Metabolism Of Weaned Piglets Under Mixed Management And Poor Housing Conditions, Joseane Penteado Rosa Gonçalves, Antonio Diego Brandão Melo, Qinnan Yang, Marllon José Karpeggiane De Oliveira, Danilo Alves Marçal, Manoela Trevisan Ortiz, Pedro Righetti Arnaut, Ismael França, Graziela Alves Da Cunha Valini, Cleslei Alisson Silva, Nate Korth, Natasha Pavlovikj, Paulo Henrique Reis Furtado Campos, Henrique Gastmann Brand, John Kyaw Htoo, João Carlos Gomes-Neto, Andrew K. Benson, Luciano Hauschild

Department of Food Science and Technology: Faculty Publications

A sanitary challenge was carried out to induce suboptimal herd health while investigating the effect of amino acids supplementation on piglet responses. Weaned piglets of high sanitary status (6.33 ± 0.91 kg of BW) were distributed in a 2 × 2 factorial arrangement into two similar facilities with contrasting sanitary conditions and two different diets. Our results suggest that increased Trp, Thr, and Met dietary supplementation could support the immune systems of piglets under a sanitary challenge. In this manner, AA+ supplementation improved the performance and metabolism of piglets under mixed management and poor sanitary conditions. No major temporal microbiome …

Apigenin Alleviates Autistic-Like Stereotyped Repetitive Behaviors And Mitigates Brain Oxidative Stress In Mice, Petrilla Jayaprakash, Dmytro Isaev, Keun-Hang Susan Yang, Rami Beiram, Murat Oz, Bassem Sadek

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Studying the involvement of nicotinic acetylcholine receptors (nAChRs), specifically α7-nAChRs, in neuropsychiatric brain disorders such as autism spectrum disorder (ASD) has gained a growing interest. The flavonoid apigenin (APG) has been confirmed in its pharmacological action as a positive allosteric modulator of α7-nAChRs. However, there is no research describing the pharmacological potential of APG in ASD. The aim of this study was to evaluate the effects of the subchronic systemic treatment of APG (10–30 mg/kg) on ASD-like repetitive and compulsive-like behaviors and oxidative stress status in the hippocampus and cerebellum in BTBR mice, utilizing the reference drug aripiprazole (ARP, 1 …

Funcpep V20: An Updated Database Of Functional Short Peptides Translated From Non-Coding Rnas, Swati Mohapatra, Anik Banerjee, Paola Rausseo, Mihnea P Dragomir, Ganiraju C Manyam, George A Calin

Faculty, Staff and Student Publications

Over the past decade, there have been reports of short novel functional peptides (less than 100 aa in length) translated from so-called non-coding RNAs (ncRNAs) that have been characterized using mass spectrometry (MS) and large-scale proteomics studies. Therefore, understanding the bivalent functions of some ncRNAs as transcripts that encode both functional RNAs and short peptides, which we named ncPEPs, will deepen our understanding of biology and disease. In 2020, we published the first database of functional peptides translated from non-coding RNAs-FuncPEP. Herein, we have performed an update including the newly published ncPEPs from the last 3 years along with the …