Life Sciences Commons^™

Increasing The Phytoremediation Efficiency Of Heavy Metal-Contaminated Soils In The Uae Using Endophytic Actinobacteria For Sustainable Agriculture, Archana Mathew Mathai

Thesis/ Dissertation Defenses

Pollution caused by heavy metals (HMs) is a major environmental concern worldwide. Human actions, agriculture practices and industrial activities can cause the release of HMs into the environment. Soil contamination with HMs may have negative effects on crops, thus disrupting the food chain and posing risks to human health. Lead (Pb) is a common pollutant found in soils when at high concentrations. Conventional land remediation techniques depending on chemical and mechanical methods are quite expensive and would lead to long-term deterioration of the ecosystems. Phytoremediation can serve as an alternative, eco-friendly approach to restore the lands for agricultural purposes. Hyperaccumulator …

Influence Of Low-Level Condensed Tannins Concentrations In Temperate Forages On Sheep Performace, F Montossi, F Liu, J Hodgson, S T. Morris, T N. Barry, D F. Risso

IGC Proceedings (1977-2023)

Results from a series of six comparative lamb grazing trials with temperate forages are summarised, and compared with results from the literature. Condensed tannin (CT) concentrations in the diet ranged from 0.12% to 0.47% on a dry matter (DM) basis. Values were generally similar for Yorkshire fog (Holcus lanatus), perennial ryegrass (Lolium perenne) and annual ryegrass (L. multiflorum), each grown with white clover (Trifolium repens), in similar circumstances. Concentrations for tall fescue (Festuca arundinacea)/white clover pastures were relatively low, but may have been artefacts. Drenching with polyethylene glycol (PEG) to bind …

Tannin Contents Of Some Indigenous Browse Plants Of Botswana, A A. Aganga, C M. Tsopito, K M. Morake

IGC Proceedings (1977-2023)

A study was carried out to evaluate the tannin content and crude protein degradation of leaves and twigs from indigenous browsable trees which provide feed for grazing livestock on natural rangelands of Botswana. These browses include the families Leguminosae (Acacias), Capparidaceae (Boscias), Combretaceae (Combretum) and Tiliaceae (Grewias). These were found to be of good nutritive value, with the leaves having a high content of crude protein (9 - 16%) and a wide range of dry matter digestibility (37 - 69.82%). The correlation between crude protein disappearance and tannin content was found to be negative (-0.582) and significant (P<0.05).

Effect Of Tannin In Animal Diet On Nitrification Rate Of Pasture Soil Under Dung Patches, J R. Crush

IGC Proceedings (1977-2023)

The rate of formation of nitrate and ammonium in the soil under dung patches was compared for dung from animals fed either ryegrass (Lolium perenne)/white clover (Trifolium repens) or ryegrass/Lotus pedunculatus forage. Tannin derived from the Lotus did not inhibit ammonification in the soil under dung but nitrate formation was strongly inhibited. The results support the theory that polyphenolics may bind to nitrite in acid soils and cause immobilisation of mineral nitrogen. Apart from their effect of reducing the proportion of dietary nitrogen excreted in urine, tannins in animal diets may make a further contribution to …

Condensed Tannins In Tropical Legumes: Concentration, Astringency And Effects On The Nutrition Of Ruminants, R Barahona, C E. Lascano, R C. Cochran, J L. Morrill, E C. Titgemeyer

IGC Proceedings (1977-2023)

A feeding trial was carried out to determine the effect of extractable condensed tannins (ECT) concentration and tannin astringency in tropical legumes on nitrogen (N) digestion by sheep. Test legumes were Desmodium ovalifolium (Do) and Flemingia macrophylla (Fm) which had similar concentrations of Extractable CT (9% DM) but tannins with different degree of astringency (Do, 0.6 and Fm, 0.3 g protein bound/g of ECT). Chopped sun-dried forage of each legume was sprayed with either water (control) or polyethylene glycol (PEG, 3.5% DM) to reduce ECT and fed to 8 sheep with ruminal and duodenal canulas arranged in a replicated 4 …

Effect Of Condensed Tannin In Lotus Corniculatus And Lotus Pedunculatus On Digestion Of Rubisco In The Rumen, W C. Mcnabb, R J. Aerts, A Brand, J S. Peters, Y L. Foo, G C. Waghorn

IGC Proceedings (1977-2023)

The in vitro precipitation of ribulose-1,5-bisphosphate carboxylase (Rubisco) by condensed tannin (CT) extracted from Lotus corniculatus and Lotus pedunculatus and the effect of these CT on the in vitro rumen degradation of Rubisco was used to compare the reactivity of these CT. The chemical structure of CT from Lotus corniculatus was homogenous with epicatechin stereochemistry and mostly procyanidin units. The CT from Lotus pedunculatus was heterogenous with mostly prodelphinidin units. The amount of CT required to precipitate all the Rubisco when total soluble leaf protein was incubated with CT from Lotus corniculatus and Lotus pedunculatus was similar. Although CT from …

Nitrogen And Fiber Digestion In Sheep Fed Fresh-Frozen And Field-Dried High And Low Tannin Sericea Lespedeza, T H. Terrill, W R. Windham, C S. Hoveland, H E. Amos, B Kouakou, S Gelaye

IGC Proceedings (1977-2023)

Field-drying reduces extractable condensed tannin (CT) of sericea lespedeza [Lespedeza cuneata (Dum-Cours) G. Don], but effects on specific bound and unbound forms of CT and the relationship to protein and fiber digestion in ruminants are unclear. Intake and digestibility of fresh-frozen and field-dried high- and low-tannin sericea were investigated in a feeding trial with sheep (Ovis spp.). Field-drying had no effect on crude protein (CP) and fiber content of sericea, but shifted CT from extractable to bound forms. Digestion coefficients for CP, neutral detergent fiber (NDF), and acid detergent fiber (ADF) were lower for high-tannin sericea than the low-tannin …

Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani

IGC Proceedings (1977-2023)

The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.

Comparison Of The In Vitro Fermentation Characteristic Of Fractionated Alfalfa And Sainfoin, L R. Mcmahon, M Goto, T A. Mcallister, G A. Jones, K J. Cheng

IGC Proceedings (1977-2023)

Alfalfa and sainfoin leaves were lyophilized and ground (A, S, respectively) or were fractionated into soluble (A_SOL, S_SOL) and insoluble (A_INS, S_INS)components and lyophilized and incubated in vitro with diluted ruminal fluid for 24h. Whole leaf and soluble fractions were also incubated with polyethylene glycol 8000 (PEG). Gas production (GP), ammonia concentration [NH3] and volatile fatty acid (VFA) production were lower (P< 0.05) from S and SSOL than from A and ASOL Insoluble fractions differed only in [NH3] (SINS < AINS, P < 0.05). Inclusion of PEG increased (P < 0.05) GP, [NH3] and VFA production from whole leaf and soluble fractions of sainfoin, but not alfalfa. Inactivating the condensed tannins in sainfoin with PEG overcame differences in degradability between these two forages.

Differences In Chemical Composition Among Provenances Of Tree Fodder Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker

IGC Proceedings (1977-2023)

The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …

Environmental Effect On New Released Genotypes Of Graminaceous And Leguminous Forage Perennial Crops, P Martiniello, G Genier, R Allerit

IGC Proceedings (1977-2023)

Genotypes of perennial leguminous (alfalfa) and graminaceous (cocksfoot and tall fescue) were evaluated for adaptability in a typical Mediterranean environment (Foggia, southern Italy). The species were field evaluated to detect biomass yield potential under different types of watering. Irrigations were applied considering rainfall and FAO cultural growth coefficient for reintegrating the 75% of the evaporated water from Class A water pan. The forage harvest was applied when the shoots of the varieties reached the 10 and 50% of flowering and heading stage for leguminous and graminaceous, respectively. A split-plot experimental design, with irrigation in the main plot, was adopted. The …

Plant Tannins- Their Role In Forage Legume Quality, J A. Alokan, V A. Aletor

IGC Proceedings (1977-2023)

Forage legumes, especially browse plants, are important in the maintenance and survival of ruminant animals. However many browse species contain high levels of polyphenolic compounds including tannins. The effects of tannins on the quality of forages are reviewed. These include reduction in palatability and intake of feeds, growth rate, digestion of fibre fractions and nitrogen utilization by animals.

Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood

IGC Proceedings (1977-2023)

An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.

Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein

IGC Proceedings (1977-2023)

Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.

A Revised Method Of Condensed Tannin Analysis In Leucaena Spp., S A. Dalzell, G L. Kerven

IGC Proceedings (1977-2023)

The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.

The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka

IGC Proceedings (1977-2023)

Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …

Perspectivas Para El Manejo Sostenible De Las Frutas De La Pasión, Stephany Hurtado Clopatosky, Pilar Rojas-Gracia, Fabián Giovanny Márquez Niño, Juan Camilo Álvarez Mahecha, Edgar Eduardo Roa Guerrero, Yuly Paola Sandoval-Cáceres, Ginna Natalia Cruz-Castiblanco

Ciencias Agropecuarias

Este libro explora el cultivo de pasifloras exóticas, especialmente la gulupa, que ha capturado mercados internacionales debido a su composición rica en antioxidantes, flavonoides y compuestos terapéuticos. Se destaca el impacto económico de su producción en Colombia, convirtiéndose en una de las frutas más exportadas junto a la granadilla y el maracuyá. Además de abordar el crecimiento del comercio global de gulupa, el libro se enfoca en la importancia de gestionar las enfermedades del cultivo y las prácticas sostenibles, el manejo integrado de la artropofauna, y la optimización de la etapa de postcosecha para preservar la calidad del fruto y …

Addicted To The Beloved? A Comparison Of Craving For And Attention To Beloved And Vape Stimuli, Caitlyn Harriman

Theses

People who are in love seem to be addicted to their beloved. People who are in love have attentional biases for the beloved and people who are addicted have attentional biases for the substance they are using. This raises the question of how similar craving and attention are for the beloved and an addictive substance. However, there are no studies that directly compared craving and attention to the beloved and addictive stimuli. The aims of the current research were to directly compare subjective emotional experiences, sustained motivated attention, and early automatic attention for the beloved and vape stimuli, in people …

Investigating The Antibacterial Properties Of Nanostructured Aluminum Surface, Quinshell Smith

Theses and Dissertations

Bacterial infections are a major public health concern. Bacterial infection can occur in any part of the body and can also cause the body to respond to its presence. Bacteria can be transferred to humans through water, food, air, and living vectors. The modes of transmission for bacterial infections are vectors, airborne, physical contact, and droplets. Current preventative measures have been put in place to combat this issue. These preventative measures include immunization of both animals and humans, personal hygiene measures, biocidal agents, and essential oils. Bacterial resistance has occurred due to these preventative measures being misused and overused. Bacteria …

An Exploration Of The Effectiveness Of Xanthorrhiza Simplicissima And Its Secondary Alkaloids As Antibiotics Against Staphylococcus Aureus With The Use Of Reserpine, Donald Bothe, Savita Chaurasia

Undergraduate Theses

Xanthorhiza simplicissima is a plant that is steeped in medical history. It has been used for hundreds of years by the Native Americans, and later by the Europeans, of Appalachia for its medicinal properties. One of the most useful medical applications of this plant are its antibiotic properties, as typified by its use as a tea that was consumed to treat mouth sores. These antibiotic properties can be attributed to some of its secondary alkaloids such as berberine, liriodenine, oxyacanthine, and magnoflorine. These compounds have been studied in other systems; however, oxyacanthine and magnoflorine have not been researched in tandem …

04.15.2024 Orsp Connect, Liz Williamson

ORED Newsletter

DMP Tool, Jeff Neal, new training module: Budget Basics, new policy Maligned Foreign Talent Recruitment

Excellence Summit 2024, Office Of Research And Sponsored Projects Operations

Excellence Summit (Research Week) Archived Proceedings

In it's 17th year, the University of the Incarnate Word, welcome all to read and review the proceedings of the annual research week. The 2024 Excellence Summit highlighted academic excellence across all schools featuring topics from nutrition studies to soil amendments, artificial intelligence, medical case studies, and music composition studies. The rich mixture of humanities, health sciences, physical sciences, education, and business can be found in this booklet. We thank all that contributed to these works and hope the community enjoys learning from today's and tomorrow's leaders in research, inquiry, and scholarship.

Mcl-1 Mediates Intrinsic Resistance To Raf Inhibitors In Mutant Braf Papillary Thyroid Carcinoma, Maria Cavallo, Jacob Yo, Kayla Gallant, Camille Cunanan, Amirali Amirfallah, Marzieh Daniali, Alyssa Sanders, Andrew Aplin, Edmund Pribitkin, Edward Hartsough

Kimmel Cancer Center Faculty Papers

Papillary thyroid carcinoma (PTC) is the most frequent form of thyroid cancer. PTC commonly presents with mutations of the serine/threonine kinase BRAF (BRAF^V600E), which drive ERK1/2 pathway activation to support growth and suppress apoptosis. PTC patients often undergo surgical resection; however, since the average age of PTC patients is under 50, adverse effects associated with prolonged maintenance therapy following total thyroidectomy are a concern. The development of mutant-selective BRAF inhibitors (BRAFi), like vemurafenib, has been efficacious in patients with metastatic melanoma, but the response rate is low for mutant BRAF PTC patients. Here, we assay the therapeutic response …

The Study On Mechanical Model Considering Optimal Self-Adaption In The Bottleneck Area, Juan Wei, Lei You, Hao Zhang, Junxi Wang

Faculty, Staff and Student Publications

It aims to solve the problem that the evacuation state of pedestrians depicted by the traditional social force model in a crowded multiexit scenario has a relatively large difference with the actual state, especially the 'optimal path' considered by the self-driving force is the problem of shortest path, and the multiexit evacuation mode depicted by the 'herd behavior' is the local optimum problem. Through in-depth analysis of actual evacuation data of pedestrians and causes of problem, a new crowd evacuation optimization model is established in order to effectively improve the simulation accuracy of crowd evacuation in a multi-exit environment. The …

Unravelling The Genetic Basis Of Schizophrenia, Clara Casey, John F. Fullard, Roy D. Sleator

Department of Biological Sciences Publications

Neuronal development is a highly regulated mechanism that is central to organismal function in animals. In humans, disruptions to this process can lead to a range of neurodevelopmental phenotypes, including Schizophrenia (SCZ). SCZ has a significant genetic component, whereby an individual with an SCZ affected family member is eight times more likely to develop the disease than someone with no family history of SCZ. By examining a combination of genomic, transcriptomic and epigenomic datasets, large-scale ‘omics’ studies aim to delineate the relationship between genetic variation and abnormal cellular activity in the SCZ brain. Herein, we provide a brief overview of …

The Descent Of Anolis: Assemblages, Convergence, And Ecomorphological Evolution, Christopher G. Anderson

Biology ETDs

The evolutionary outcomes we observe in modern organisms, particularly associations between phenotypes and environments, have been and remain an invaluable tool in interpreting the biological phenomena that shape our world. In the following chapters, I leverage a comprehensive phylogenetic and morphological dataset for 351 species comprising a diverse group of arboreal lizards, the Anolis clade, to explore how communities and morphologies evolve. First, I characterized patterns among coexisting lineages of Anolis to reveal a general pattern of phylogenetic relatedness among lineages comprising assemblages and identify differences attributable to a biogeographic variable. Then I examined the morphological consequences associated with the …

Safety Profile And Adverse Event Management For Futibatinib, An Irreversible Fgfr1-4 Inhibitor: Pooled Safety Analysis Of 469 Patients, Funda Meric-Bernstam, Antoine Hollebecque, Junji Furuse, Do-Youn Oh, John A Bridgewater, Bailey Anderson, Volker Wacheck, Lipika Goyal

Faculty, Staff and Student Publications

PURPOSE: Futibatinib, a covalently-binding inhibitor of fibroblast growth factor receptor (FGFR)1-4 gained approval for the treatment of refractory, advanced intrahepatic cholangiocarcinoma (iCCA) harboring an FGFR2 fusion/other rearrangement. An integrated analysis was performed to evaluate safety and provide guidance on the management of futibatinib-associated adverse events (AEs) in patients with unresectable/metastatic tumors, including iCCA.

PATIENTS AND METHODS: Data from three global phase I or II studies of futibatinib (NCT02052778; JapicCTI-142552) were pooled. AEs were graded per NCI CTCAE v4.03, where applicable. Safety was analyzed for patients receiving any futibatinib starting dose (overall population) and in those receiving the approved starting dose …

Multi-Omics Analysis Reveals Immune Features Associated With Immunotherapy Benefit In Patients With Squamous Cell Lung Cancer From Phase Iii Lung-Map S1400i Trial, Edwin Roger Parra, Jiexin Zhang, Dzifa Yawa Duose, Edgar Gonzalez-Kozlova, Mary W Redman, Ganiraju C Manyam, Jianhua Zhang, Xingzhi Song, Rossana Lazcano, Mario L Marques-Piubelli, Caddie Laberiano-Fernandez, Frank Rojas, Baili Zhang, Len Taing, Aashna Jhaveri, Jacob Geisberg, Jennifer Altreuter, Franziska Michor, James Provencher, Joyce Yu, Ethan Cerami, Radim Moravec, Kasthuri Kannan, Rajyalakshmi Luthra, Gheath Alatrash, Hsin-Hui Huang, Hui Xie, Manishkumar Patel, Kai Nie, Jocelyn Harris, Kimberly Argueta, James Lindsay, Roshni Biswas, Stephen Van Nostrand, Seunghee Kim-Schulze, Jhanelle E Gray, Roy S Herbst, Ignacio I Wistuba, Scott Gettinger, Karen Kelly, Lyudmila Bazhenova, Sacha Gnjatic, J Jack Lee, Jianjun Zhang, Cara Haymaker

Faculty, Staff and Student Publications

PURPOSE: Identifying molecular and immune features to guide immune checkpoint inhibitor (ICI)-based regimens remains an unmet clinical need.

EXPERIMENTAL DESIGN: Tissue and longitudinal blood specimens from phase III trial S1400I in patients with metastatic squamous non-small cell carcinoma (SqNSCLC) treated with nivolumab monotherapy (nivo) or nivolumab plus ipilimumab (nivo+ipi) were subjected to multi-omics analyses including multiplex immunofluorescence (mIF), nCounter PanCancer Immune Profiling Panel, whole-exome sequencing, and Olink.

RESULTS: Higher immune scores from immune gene expression profiling or immune cell infiltration by mIF were associated with response to ICIs and improved survival, except regulatory T cells, which were associated with worse …

Assessment Of Potentially Toxic And Mineral Elements In Paddy Soils And Their Uptake By Rice (Oryza Sativa L) With Associated Health Hazards In District Malakand, Pakistan, Ghulam Saddiq

Faculty, Staff and Student Publications

Rice, a primary food source in many countries of the world accumulate potentially harmful elements which pose a significant health hazard to consumers. The current study aimed to evaluate potentially toxic and mineral elements in both paddy soils and rice grains associated with allied health risks in Malakand, Pakistan. Rice plants with intact root soil were randomly collected from paddy fields and analyzed for mineral and potentially toxic elements (PTEs) through inductively coupled plasma optical emission spectrometry (ICP‒OES). Through deterministic and probabilistic risk assessment models, the daily intake of PTEs with allied health risks from consumption of rice were estimated …