Life Sciences Commons^™

The Effects Of A Guided Mindful Walk On Mental Health In University Students, Ashley V. Burdick, Sarah M. Camhi

International Journal of Exercise Science

International Journal of Exercise Science 17(5): 590-601, 2024. College campuses in the United States are experiencing high levels of mental distress without adequate psychological resources to address the need. In addition, the majority of university students do not meet the physical activity guidelines for mental and physical health. Effective and time efficient resources are needed to address poor mental health and low physical activity among university students on college campuses. Mindful walking may be a promising solution. The purpose was to 1) measure change in mental health and 2) estimate physical activity from participation in a guided mindful walk in …

The Santa Clara, 2024-04-26, Santa Clara University

The Santa Clara

No abstract provided.

Trna Anticodon Cleavage By Target-Activated Crispr-Cas13a Effector, Ishita Jain, Matvey Kolesnik, Konstantin Kuznedelov, Leonid Minakhin, Natalia Morozova, Anna Shiriaeva, Alexandr Kirillov, Sofia Medvedeva, Alexei Livenskyi, Laura Kazieva, Kira S Makarova, Eugene V Koonin, Sergei Borukhov, Konstantin Severinov, Ekaterina Semenova

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Type VI CRISPR-Cas systems are among the few CRISPR varieties that target exclusively RNA. The CRISPR RNA–guided, sequence-specific binding of target RNAs, such as phage transcripts, activates the type VI effector, Cas13. Once activated, Cas13 causes collateral RNA cleavage, which induces bacterial cell dormancy, thus protecting the host population from the phage spread. We show here that the principal form of collateral RNA degradation elicited by Leptotrichia shahii Cas13a expressed in Escherichia coli cells is the cleavage of anticodons in a subset of transfer RNAs (tRNAs) with uridine-rich anticodons. This tRNA cleavage is accompanied by inhibition of protein synthesis, thus …

Ua12/2/2 Talisman: Pulse, Wku Student Affairs

WKU Administration Documents

Spring 2024 Talisman:

• Hunter, Leah. Pulse
• Muscutt, Carson. High on Bluegrass – Jordan Riehm
• Meyers, Jeffrey. Science of Movement – Exercise Science
• Murray, Debra. Pasture to Plate – Agriculture
• Murray, Debra. Vegetarian Tortilla Soup
• Coffee, Bobbi. Passion, Pain, Curiosity – Innovation Campus
• Story, Miriam. Drafts of a Postcard (October 2023)
• Johnston, Katelyn. Beyond Sound – Deaf
• Hunter, Leah. One Good Week – Madison Weis
• Alali, Cecilia. Crafted Cadence – Speech Team
• Blankenship, Alisa. Loving You
• Sandlin, Ellie. Here After – Death
• Booth, Pandora. Rooted in Rhythm – Step Shows
• Booth, Pandora. Horoscopes
• Pulse Playlist

Ablation Of Sam50 Is Associated With Fragmentation And Alterations In Metabolism In Murine And Human Myotubes, Bryanna Shao, Mason Killion, Ashton Oliver, Chia Vang, Faben Zeleke, Kit Neikirk, Zer Vue, Edgar Garza‐Lopez, Jian‐Qiang Shao, Margaret Mungai, Jacob Lam, Qiana Williams, Christopher T. Altamura, Aaron Whiteside, Kinuthia Kabugi, Jessica Mckenzie, Maria Ezedimma, Han Le, Alice Koh, Estevão Scudese, Larry Vang, Andrea G. Marshall, Amber Crabtree, Janelle I. Tanghal, Dominique Stephens, Ho‐Jin Koh, Brenita C. Jenkins, Sandra A. Murray, Anthonya T. Cooper, Clintoria Williams, Steven M. Damo10 M. Damo, Melanie R. Mcreynolds, Jennifer A. Gaddy Gaddy, Celestine N. Wanjalla, Heather K. Beasley, Antentor Hinton Jr.

Biology Faculty Research

The sorting and assembly machinery (SAM) Complex is responsible for assembling β‐barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, and Sam50, the SAM complex connects the inner and outer mitochondrial membranes by interacting with the mitochondrial contact site and cristae organizing system complex. Sam50, in particular, stabilizes the mitochondrial intermembrane space

bridging (MIB) complex, which is crucial for protein transport, respiratory chain complex assembly, and regulation of cristae integrity. While the role of Sam50 in mitochondrial structure and metabolism in skeletal muscle remains unclear, this study aims to investigate its impact. Serial block‐face‐scanning electron microscopy and computer‐assisted …

Terrestrial Birth And Body Size Tune Ucp1 Functionality In Seals, Michael J. Gaudry, Jane Khudyakov, Laura Pirard, Cathy Debier, Daniel Crocker, Paul G. Crichton, Martin Jastroch

Pacific Faculty Work

The molecular evolution of the mammalian heater protein UCP1 is a powerful biomarker to understand thermoregulatory strategies during species radiation into extreme climates, such as aquatic life with high thermal conductivity. While fully aquatic mammals lost UCP1, most semiaquatic seals display intact UCP1 genes, apart from large elephant seals. Here, we show that UCP1 thermogenic activity of the small-bodied harbor seal is equally potent compared to terrestrial orthologs, emphasizing its importance for neonatal survival on land. In contrast, elephant seal UCP1 does not display thermogenic activity, not even when translating a repaired or a recently highlighted truncated version. Thus, the …

Investigating The Adherence Of Maternal Prenatal Vitamin Supplementation In Sarasota, Florida, Priya Darbhanga, Kaisha Mcmanus

BIO4960: Practicum in Biology I

Prenatal supplementation plays a critical role in supporting a healthy pregnancy and lowering the risk of developmental problems in children. Many expecting mothers do not consume the recommended amounts of such vitamins or any at all. This study aims to identify and understand the factors that contribute to prenatal vitamin adherence amongst women at the Sarasota Medical Pregnancy Center located in Sarasota, Florida. Paper-based questionnaires were administered to all willing participants to assess the current adherence of women to prenatal supplementation and discover the reasonings for such decisions. The primary contributors to adherence to prenatal vitamins were related to a …

Structural Homology Of Mite Profilins To Plant Profilins Is Not Indicative Of Allergic Cross-Reactivity, Andrea O'Malley, Sahana Sankaran, Avery Carriuolo, Kriti Khatri, Krzysztof Kowal, Maksymilian Chruszcz Ph.D.

Faculty Publications

Structural and allergenic characterization of mite profilins has not been previously pursued to a similar extent as plant profilins. Here, we describe structures of profilins originating from Tyrophagus putrescentiae (registered allergen Tyr p 36.0101) and Dermatophagoides pteronyssinus (here termed Der p profilin), which are the first structures of profilins from Arachnida. Additionally, the thermal stabilities of mite and plant profilins are compared, suggesting that the high number of cysteine residues in mite profilins may play a role in their increased stability. We also examine the cross-reactivity of plant and mite profilins as well as investigate the relevance of these profilins …

Nampt, Il-6, And Vaspin Gene Expressions And Serum Protein Levels In Type 2 Diabetes Mellitus And Related Complication, Süheyla Pınar Çelik, Damla Nur Parıltı, Leyla Açık, Mehmet Muhittin Yalçın, İlhan Yetkin, Eldeniz Yunusov

Turkish Journal of Biology

Type 2 diabetes (T2DM) is the most common type of diabetes and occurs due to insufficient insulin secretion or inability to use existing insulin and the effects of environmental factors. Although there are many studies on the pathophysiology of T2DM, the mechanisms playing a role in the pathogenesis of insulin resistance and pancreatic beta-cell dysfunction have not been fully elucidated. Some adipokines secreted from adipose tissue, which are the primary regulators of insulin resistance, affect immune and inflammatory functions. Altered adipokine profiles have been observed in obesity and T2DM, leading to severe metabolic risks and changes in insulin sensitivity. This …

Evolution In Complex Systems: A Computational Prespective, Andisheh Dadashi

Computer Science ETDs

As we are entering the era of multi-omic datasets, it is increasingly evident that the complexities of natural systems exceed the capabilities of current computational methodologies. My dissertation research aims to reduce this gap by using computational frameworks to investigate the evolution of complex systems. I apply a model of metabolic networks to predict human embryonic metabolism during peri-implantation (Chapter I), offering insights into the foundations of early human development. This dissertation further examines the basis of the phenotypic evolution of polygenic traits when subjected to directional (Chapter II) and temporally varying environmental shifts (Chapter III), revealing the roles of …

Linking Reactive Nitrogen Species Within The Vadose Zone With Surface Irrigation, Kaitlyn Richards

Honors Program: Senior Projects (Public)

This study investigated the relationship between irrigation methods and the mobilization of nitrogen in the vadose zone, focusing on soil properties, water dynamics, and nitrogen species distribution. Data collected from dryland and pivot-irrigated soil cores reveal distinct patterns in water content, bulk density, soil texture, pH, conductivity, and nitrogen concentrations. Results indicated that water content has a major influence in nitrogen availability and microbial activity. Nitrate-N and ammonium-N concentrations varied between dryland and pivot soils, with notable disparities observed. In dryland soils, nitrate-N concentrations varied widely across depth intervals, with notable accumulation in deeper layers, while ammonium-N concentrations exhibited a …

Research Proposal: The Effects Of Creatine Supplementation On High School Athletes, Rebecca Kerr, Lacaya Evans

Department of Nutrition Student Projects

Creatine is commonly used among the adolescent athletic population. The prevalence rates are higher for anaerobic sports, for example, wrestling, football, and hockey. While creatine is a supplement of choice for many young athletes, its effects on performance are not well researched. Though studies point toward it being effective and safe, with few negative side effects documented, further analysis is needed to ensure it will not affect teens’ development. Therefore, this study aims to examine how creatine supplementation by high school athletes affects muscle development and its safety. Distributing creatine supplements to youth raises ethical concerns. Therefore, this study will …

Investigating Tumor-Stroma Interactions During Ovarian Cancer Metastasis, Angela Marie Schab

Arts & Sciences Graduate Student Theses and Dissertations

The majority of ovarian cancer patients have metastatic disease throughout the peritoneal cavity at the time of diagnosis, at which point the five-year survival is only around 30%. However, no effective therapy exists to prevent or treat ovarian cancer metastasis. To implant into the peritoneal cavity, ovarian cancer cells must attach to the mesothelial cell layer-a monolayer of cells that surrounds all organs of the peritoneal cavity- clear the mesothelial cell layer and invade into the underlying stroma, which hosts fibroblasts and extracellular matrix (ECM). The tumor microenvironment (TME) plays an important role in supporting metastasis and thus is a …

Determining The Role Of Ddr2 In Acquired Chemo-Resistance In Ovarian Cancer, Alyssa Oplt

Arts & Sciences Graduate Student Theses and Dissertations

Ovarian cancer is the 5th leading cause of cancer death in women with a 5-year survival rate around 30%. Up to 80% of ovarian cancer patients experience disease recurrence and the majority of those cases are resistant to treatment. Therefore, there is a need to understand the mechanisms of chemo-resistance in order to create new therapies and increase patient survival. To accomplish this, I first developed novel, chemo-resistant, syngeneic mouse ovarian cancer cell lines by serial passaging ovarian cancer cells through mice and treating with increasing doses of chemotherapy. These cell lines fill a need in the field for matched …

Are Objects Oriented Towards Your Dominant Hand Easier To Recognize?, Lauren A. Miller

Honors Scholar Theses

Previous research demonstrated that conceptual knowledge of manipulable objects is partially grounded in the motor system. For instance, explicitly retrieving knowledge about how a manipulable object is interacted with (e.g. pinched or clenched?) is easier for right handed people if the object is oriented so that it more easily affords grasping with the right hand (Chrysikou et al., 2017). We tested if motor experience through handedness also influences participants’ abilities to name objects that are commonly grasped with their dominant hand. Participants were asked to name images of graspable objects whose handles were oriented toward either their left or right …

Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang

Department of Agronomy and Horticulture: Faculty Publications

DREB has been reported to be involved in plant growth and response to environmental factors. However, the function of DREB in growth and development has not been elucidated in alfalfa (Medicago sativa L.), a perennial tetraploid forage cultivated worldwide. In this study, an ortholog of MtDREB1C was characterized from alfalfa and named MsDREB1C accordingly. MsDREB1C was significantly induced by abiotic stress. The transcription factor MsDREB1C resided in the nucleus and had self-transactivation activity. The MsDREB1C overexpression (OE) alfalfa displayed growth retardation under both long-day and short-day conditions, which was supported by decreased MsGA20ox and upregulated MsGA2ox in the OE …

A Novel Algorithm For The Virtual Screening Of Extensive Small Molecule Libraries Against Ercc1/Xpf Protein-Protein Interaction For The Identification Of Therapeutic Resistance-Bypassing Small Anticancer Molecules, Salma Ghazy, Lalehan Oktay, Serdar Durdagi

Turkish Journal of Biology

Background and aim:Cancer cell’s innate chemotherapeutic resistance continues to be an obstacle in molecular oncology. This theory is firmly tied to the cancer cells’ integral DNA repair mechanisms continuously neutralizing the effects of chemotherapy. Amidst these mechanisms, the nuclear excision repair pathway is crucial in renovating DNA lesions prompted by agents like Cisplatin. The ERCC1/XPF complex stands center-stage as a structure specific endonuclease in this repair pathway. Targeting the ERCC1/XPF dimerization brings forth a strategy to augment chemotherapy by eschewing the resistance mechanism integral to cancer cells. This study tracks and identifies small anticancer molecules, with ERCC1/XPF inhibiting potential, within …

Non-Small Cell Lung Cancer Treatment: Current Status Of Drug Repurposing And Nanoparticle-Based Drug Delivery Systems, Tugba Gul Inci, Serap Acar, Di̇lek Balik

Turkish Journal of Biology

Drug repurposing is the strategy of drug utilization for a treatment option other than the intended indications and has increased over the past decades, especially within cancer nanomedicine. Cancer nanomedicine has been facilitated through nanoparticle-based (NP-based) delivery systems which can combat NSCLC via recent advances in nanotechnology and apply its benefits to existing drugs. An effective therapeutic solution could be gained via repurposing and accelerated via NP-based drug delivery systems. This review aims to present an overview of NSCLC treatments with a special focus on drug repurposing with details of clinical study advances and NP-based drug delivery systems for NSCLC. …

Circ-Mettl15 Stimulates The Aggressive Behaviors Of Papillary Thyroid Cancer Cells By Coordinating The Mir-200c-3p/Xiap Axis, Yuhao Huang, Xinyu Zeng, Yanling Cai, Yan Yang, Yujie Zhang, Yiqi Ma, Suping Li

Turkish Journal of Biology

Background and objective: Thyroid cancer is the most common malignant tumor in the endocrine system. Papillary thyroid carcinoma (PTC) accounts for the vast majority of cases in this cancer. Recently, the vital role of circular RNA (circRNA) has been acknowledged in various cancers. However, to date, few studies have examined the roles of circRNAs in PTC. Therefore, this research aimed at exploring the function and mechanism of circ-methyltransferase-like 15 (METTL15) in PTC cells.Methods: First, quantitative measurements of circ-METTL15, miR-200c-3p, and X-linked inhibitor of apoptosis protein (XIAP) in PTC cells were completed with reverse transcription-quantitative polymerase chain reaction or Western blot …

Mir-770-5p-Induced Cellular Switch To Sensitize Trastuzumab Resistant Breast Cancer Cells Targeting Her2/Egfr/Igf1r Bidirectional Crosstalk, Senem Noyan, Bala Gür Dedeoğlu

Turkish Journal of Biology

Background/aim: Studies highlighted the bidirectional crosstalk between the HER family members in breast cancer as resistance mechanism to anti-HER agents. Cross-signaling between HER2/EGFR and ER/IGF1R could play role in the development of resistance to therapeutics hence stimulating cell growth. To overcome this resistance, combined therapies targeting both pathways simultaneously have been proposed as an effective strategy. The involvement of miRNAs in resistance of targeted therapies like trastuzumab was demonstrated in recent studies. Hence the regulation of miRNAs in resistance state could reverse the cell behaviour to drugs. Previously we found that overexpression of miR-770-5p downregulated AKT and ERK expression through …

Physical Activity, Cardiorespiratory Fitness And All-Cause Mortality, Jorin Dane Larsen

Theses and Dissertations

Introduction Both elevated levels of physical activity (PA) and high cardiorespiratory fitness (CRF) are associated with a decreased risk of all-cause mortality. The degree to which an individual's PA level and CRF status interact to influence mortality is not currently fully understood. This study investigated whether PA and CRF were independently related to all-cause mortality, and to what degree PA was associated with improved mortality risk in individuals with low CRF. Methods This is a prospective cohort study using health assessments on individuals gathered between 1975 and 2002. Health assessment data were matched with the National Death Registry to assess …

Investigating The Role Of Tryptophan In The Native Photo-Crosslinking Of A Cys-Containing Transmembrane Helix To A Membrane Lipid Double Bond, Michael Jay Telehany

Undergraduate Honors Theses

Our research group has previously discovered that a Cys residue within a transmembrane (TM) helix can directly photo-crosslink to a membrane lipid via an oxythiolation addition reaction to the double bond within the lipid, with a reaction yield of 10-20%. The pH-low Insertion Peptide (pHLIP) serves as our model TM helix, while the POPC lipid bilayer is our model membrane. We had long believed that the Trp residues within the sequence of pHLIP served as the key chromophore for this reaction, and thus they were essential for the formation of the TM helix-lipid adduct. To test this hypothesis, I synthesized …

The Impact Of Family Functioning Factors On Smartphone Addiction And Phubbing Among Muslim Adolescents In Thailand

Faculty, Staff and Student Publications

BACKGROUND: While there is research on protective factors against smartphone addiction (SA) and phubbing, which impact adolescents' physical, psychological, interpersonal, and academic well-being, focused studies on these issues among Thai Muslim students in Southern Thailand remain scarce.

OBJECTIVES: To bridge this gap, this research aimed to explore the influence of five family functioning factors-discipline, communication and problem-solving (CPS), relationship, emotional status, and family support-guided by family systems theory and the McMaster Model, on SA and phubbing.

METHODS: Data from 825 Thai Muslim adolescent secondary school students (Female N = 459 (55.7%), M

RESULTS: Significant connections were identified between family functioning …

Dissecting The Role Of Chromatin Remodeling In Direct Lineage Reprogramming, Kunal Jindal

Arts & Sciences Graduate Student Theses and Dissertations

Direct reprogramming, the direct forced conversion of one somatic cell type to another has widespread applications in regenerative medicine. Owing to its highly complex nature, discovery and validation of new factors for direct reprogramming is challenging and many current protocols suffer from low conversion efficiency, failing to create mature cell types comparable to their in vivo counterparts. Recent advances in single-cell have coupled cell ancestry and transcriptomic state measurement in single-cell RNA sequencing (scRNA-seq) assays. In reprogramming and development, these have allowed mapping lineage specific transcriptional changes both before and during cell fate specification. These methods work by encoding lineage …

Net Fluxes Of Broadband Shortwave And Photosynthetically Active Radiation Complement Ndvi And Near Infrared Reflectance Of Vegetation To Explain Gross Photosynthesis Variability Across Ecosystems And Climate, Kanishka Mallick, Joseph Verfaillie, Tianxin Wang, Ariane Arias Ortiz, Daphne Szutu, Koong Yi, Yanghui Kang, Robert Shortt, Tian Hu, Mauro Sulis, Zoltan Szantoi, Gilles Boulet, Joshua B. Fisher, Dennis Baldocchi

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

A significant challenge in global change research is understanding how vegetation interacts with the environment to influence ecosystem gross primary productivity (GPP) through carbon assimilation. One emerging objective is to consistently predict GPP fluctuations worldwide by establishing a robust scaling relationship between GPP measured at flux towers and satellite spectral reflectance data. However, a major hurdle in achieving this goal is the discrepancy in spatial resolution between early satellite measurements and eddy flux measurements. By using a large set of growing season data covering 100 site-years in North and Central America, we explored the potential of transforming incident and reflected …

New Insights Into Macrophage Polarization And Its Prognostic Role In Patients With Colorectal Cancer Liver Metastasis, Isha Khanduri, Harufumi Maki, Anuj Verma, Riham Katkhuda, Gayathri Anandappa, Shanyu Zhang, Zhimin Tong, Luisa M Solis Soto, Akshaya Jadhav, Ignacio I Wistuba, David Menter, Scott Kopetz, Edwin R Parra, Jean-Nicolas Vauthey, Dipen M Maru

Faculty, Staff and Student Publications

Background: As liver metastasis is the most common cause of mortality in patients with colorectal cancer, studying colorectal cancer liver metastasis (CLM) microenvironment is essential for improved understanding of tumor biology and to identify novel therapeutic targets.

Methods: We used a multiplex immunofluorescence platform to study tumor associated macrophage (TAM) polarization and adaptive T cell subtypes in tumor samples from 105 CLM patients (49 without and 56 with preoperative chemotherapy).

Results: CLM exhibited M2 macrophage polarization, and helper T cells were the prevalent adaptive T cell subtype. The density of total, M2 and TGFβ-expressing macrophages, and regulatory T cells was …

Game Night! Assessment Of Playability And Learning Effectiveness Of Active Learning Games In Human Anatomy And Physiology Students, Hannah Brady

Honors Theses

Human Anatomy and Physiology (HAP) courses are a requirement for many students pursuing an education in healthcare. Students tend to dread these courses for their abundance of information in such a short amount of time. These courses tend to have a low success rate because students are not adequately prepared for this upper-level class that requires intense dedication and studying. This problem for students is extreme as these courses are required for most pre-health undergraduate students, and these courses may predict their success in their future education. HAP professors are constantly trying to figure out better ways to get students …

Comparison Of Self-Reported Physical Activity And Wearable Device-Xiaomi Mi2 Band In Preadolescent School Children-A Pilot Crossesctional Study, Aysha Almas, Shiraz Hashmi, Adil Elahi, Om Parkash, Sania Sabir, Romaina Iqbal

International Journal of Exercise Science

International Journal of Exercise Science 17(7): 565-575, 2024. The purpose of this study is to compare physical activity measured by the wearable device Xiaomi Mi2 Band and self-reported physical activity by YPAQ in preadolescent school children. A pilot cross-sectional study was performed on a subsample (5%=n=60) of preadolescent children within a parallel-group feasibility intervention trial (n=1000). All children aged 9-11 years enrolled in the schools were included in the study and children with any physical disability were excluded. Self-reported physical activity was recorded through the modified version of the Youth Physical Activity Questionnaire (YPAQ) and automatic physical activity was measured …

Effects Of Resistance Training Techniques On Metabolic Responses In Trained Males, Alysson Enes, Ragami Chaves Alves, Vinicius Zen, Danilo Fonseca Leonel, Gustavo Oneda, Luis H. B. Ferreira, Luciano R. Guiraldelli, Roberto Simão, Guillermo Escalante, Anderson Z. Ulbrich, Tácito P. Souza-Junior

International Journal of Exercise Science

International Journal of Exercise Science 17(2): 576-589, 2024. This study investigated the effects of bi-set, drop-set and traditional resistance training (RT) techniques on metabolic responses in resistance-trained males. Fifteen trained males (age 29.7 ± 6.1 years; body mass 83.4 ± 7.6 kg; RT experience 11.4 ± 6.7 years; one-repetition maximum (1RM) barbell bench press: body mass ratio 1.4 ± 0.1 a.u.) were assigned to three experimental conditions, in a randomized crossover design. The experimental conditions were bi-set (3x10 repetitions at 70%1RM in barbell bench press followed by 10 repetitions at 60%1RM in incline bench press), drop-set (3x10 repetitions at …

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxxii. Barbaracurus Hofereki Sp. N. From Djibouti, František Kovařík

Euscorpius

Barbaracurus hofereki sp. n. from Djibouti is described and compared with other species of the genus. B. hofereki sp. n. is the only species of the genus with pedipalp movable finger with 8 rows of granules and pectinal tooth count 27–28 in female. Also, its pedipalp chela with very narrow manus (chela length/width ratio 6.07 in female), narrower than in all other African species of the genus. A map of the distribution of the genus in the Horn of Africa and the Arabian Peninsula is provided.