Life Sciences Commons^™

Barriers To Participation In Research Sae Projects And Ffa Agriscience Fair Competitions, Jessica Hall

Graduate Theses and Dissertations

Agricultural education plays a pivotal role in preparing students for careers in the ever-evolving field of agriculture. This study investigates the professional development and training needs of school-based agricultural education (SBAE) teachers in the context of research SAE projects and participation in FFA agriscience fair competitions. Through a comprehensive literature review and empirical inquiry, this study aims to identify teachers' confidence levels, perceived importance of tasks, professional development needs and barriers hindering student and teacher involvement in these activities. The research objectives were structured to address key aspects of SBAE teacher preparedness and student engagement. Findings reveal areas where teachers …

Molecular Characterization Of Stress Response In Western Honey Bee (Apis Mellifera), Faizan Tahir

Master's Theses

Honey bees are incredibly important for the reproduction of flowering plants and the sustainability of agricultural ecosystems. However, they face various stressors such as pesticides, pathogens, habitat loss, and climate change. Extensive research has been conducted to understand how bees respond to these stressors. Scientists have discovered that honey bees exhibit complex physiological and behavioral responses to stress at individual and colony levels. Stress can have a significant impact on their immune function, foraging behavior, and reproductive success (Decourtye et al., 2010). Understanding bee responses to stress is crucial for several reasons. Firstly, honey bees are vital for the pollination …

Salinity Tolerance Of Flowering Rush, Butomus Umbellatus, Andrew Coomes

Master's Theses

Flowering rush is an invasive aquatic plant in North America that causes deleterious effects to native ecosystems. There are two cytotypes, a triploid and diploid, and multiple genotypes contained within the diploid cytotype currently established in the U.S. Despite its presence in river drainages connected to estuarine areas, documentation on the salinity tolerance of flowering rush is scarce. Currently, information is limited to anecdotal reports suggesting intolerance to saline conditions. A better understanding of the osmotic tolerance of this species is essential to providing insight into its’ invasive range and better inform management efforts. These studies investigated vegetative propagule germination …

The Assessment Of Autonomic Function In Young Adults With An Elevated Risk Of Metabolic Syndrome, Ta'quoris Newsome

Master's Theses

Metabolic syndrome (MetS) is a complex medical condition defined by the co-occurrence of multiple cardiometabolic risk factors. Despite the well-defined profile of autonomic function in individuals with MetS, autonomic function in those with an elevated risk of developing MetS remains unclear. Our study aimed to shed light on the progression of cardiometabolic disease and its impact on autonomic function. To investigate, twenty participants, ten of whom were classified as having an elevated risk of developing MetS based on identification of risk factors using NCEP ATP III criteria, completed a two-visit study protocol. In the second visit, spontaneous cardiovagal baroreflex sensitivity …

Rare Case Of Open Comminuted Scaphoid Fracture Dislocation: Treatment With Free Bone Graft And Locking Plate, Makoto Fukuda, Masahiro Miyashita, Masataka Yasuda

Children’s Nutrition Research Center Staff Publications

To the best of our knowledge, there are no reports in the literature of an open comminuted scaphoid fracture dislocation. We present such a rare case. The case report illustrates the case of a 58-year-old right-handed press operator who injured his left wrist when his hand got caught in a press machine. He received initial treatment at another hospital and was subsequently referred to our hospital. Eight days after the injury, surgery was performed under the brachial plexus block. Successful bone fusion was achieved through volar locking plate fixation, primary free bone grafting from the radius, and Kirschner wire fixation. …

Understanding Taf13 (Tata Box-Binding Protein-Associated Factor 13) Upregulation In Eukaryotic Cells, Selin Kaplanoglu

Honors Theses

TATA-binding protein (TBP) and TBP-associated factors (Tafs) comprise RNA Polymerase II (RNA Pol II) pre-initiation complex. This universal component carefully controls the transcriptional initiation process. One of the Tafs, Taf13, also plays an important role in the regulation of RNA Pol II transcription initiation which is evolutionarily conserved from yeast to humans. It is found that Taf13 is overexpressed in cancer cells, although the exact mechanism that is responsible for this overexpression is unclear. Our hypothesis suggests that targeted degradation by the 26S proteasome via ubiquitylation [Ubiquitin-Proteasome System (UPS)] may be the mechanism that regulates the stability of Taf13. To …

Elucidation Of The Overexpression Of Taf2 In Eukaryotic Cells, Morgan Osborn

Honors Theses

Through several studies, Taf2 has been found to be upregulated in various cancer cells. However, the mechanism through which this increased expression of Taf2 occurs remains unknown. As evolutionarily conserved ubiquitin-proteasome system (UPS) maintains protein homeostasis for normal cellular function, we hypothesized that stability of Taf2 may be regulated by this UPS and this UPS may be dysregulated in cancer cells causing overexpression of Taf2. To test our hypothesis, we assessed the role of the UPS in the regulation of the stability of Taf2 by 26S proteasome-mediated degradation. To do so, we performed molecular experiments mainly through two steps: 1st …

Associations Between Gut Microbiome Metabolomics And Endometriosis Risk, Progression, And Non-Invasive Diagnosis: A Review, Marlee Sanders

Honors Theses

Endometriosis is a gynecological condition that affects 1 in 10 women of reproductive age, is characterized by growth of endometrial tissue outside of the uterus, and encompasses metabolic, immunologic, and endocrine dysfunction. Despite its significant impact, endometriosis remains inadequately studied within the realm of women's health, emphasizing the crucial need for innovative approaches in the analysis and diagnosis of this complex condition. The cause of endometriosis is unknown however it could be influenced by genetics, environmental factors, diet, lifestyle, and notably composition of the gut microbiome. Recent scholarly interest has sparked inquiries into the correlations between endometriosis and the gut …

Determining The Antimicrobial Activity And Permeabilizing Effects Of Polyunsaturated Fatty Acids And Piscidins On Vibrio Parahaemolyticus And Vibrio Vulnificus, Christina Harris

Honors Theses

Antimicrobial peptides (AMPs) are found in all organisms and have gained attention for their effectiveness against a variety of pathogens. Piscidins are AMPs that were discovered in mast cells of vertebrates, mostly within marine animals, and are an important part of the innate immune system due to their potent antimicrobial properties. Piscidin 1 (P1) and piscidin 3 (P3) are found within sea bass and have different antimicrobial activities. P1 exhibits strong membrane disruptive properties, while P3 goes through the membrane to the targeted DNA. These piscidins can experience metalation with copper (Cu2+) which enhances their antimicrobial activity in vitro (P1-Cu2+ …

A Review Of Rheb Activation Of Mtorc1 And The Great Mystery Of One Missing Gef, Jack Gregory

Senior Honors Theses

The mTORC1 pathway is involved in the regulation of cell growth and translation. The pathway has a complex web of activators and inhibitors to activate mTORC1. mTORC1 is regulated via a small GTPase called Rheb, which interacts directly with mTORC1. This GTPase and its GTPase activating protein (GAP), TSC1/2, have been widely studied to understand how the variety of regulators of mTORC1 interact with these proteins. Despite this, the guanine nucleotide exchange factor (GEF) of Rheb has yet to be identified. This review broadly analyzes Rheb and mTORC1, their structures, regulations, and interactions, and explores the mystery of the missing …

Potential Abatement Of Bay Nettle (Chrysaora Chesapeakei) Blooms Through Polyp Removal Within Lagoon Communities In Barnegat Bay, Nj, Anthony Luca Tamberelli

Theses, Dissertations and Culminating Projects

Jellyfish blooms have gained increased attention in recent decades due to their negative impacts on ecosystems and human activities. Urban development, pollution, and overfishing are some of many possible impacts that can bolster the frequency, duration, and intensity of jellyfish blooms. In Barnegat Bay, NJ, USA, the invasive bay nettle Chrysaora chesapeakei exerts top-down predation pressure on zooplankton, including ctenophores, hydrozoans, crustaceans, and ichthyoplankton. Bay nettle populations rely on hard substrates for larval settlement and polyp clonal growth. To determine whether abatement efforts targeted at bay nettle polyps can disrupt the life cycle and limit medusae populations, a three-year sampling …

The Role Of The Transcription Factor Cebpa In Regulating Lung Alveolar Type 2 Cell Fate In Vivo, Dalia Hassan

Dissertations and Theses (Open Access)

Cell plasticity can extend across all possible cell types, yet it naturally diminishes as cells progress through differentiation. This plasticity can be reactivated during injury repair, engaging developmental flexibility. Our investigations reveal the critical role of the transcription factor (TF) CEBPA, specific to lung alveolar type 2 (AT2) cells, in modulating AT2 cell plasticity within the mouse lung. We demonstrate that CEBPA constrains AT2 cell plasticity by promoting the AT2 differentiation program and recruiting the lineage-specific TF NKX2-1. Without CEBPA, AT2 cells, in both neonatal and mature, show a diminished AT2 program; however, only neonatal cells re-activate the SOX9 progenitor …

Mismatch Repair Deficient Neoantigen And Associated Circulating T-Cell Receptor Repertoires In Lynch Syndrome, Ana Bolivar

Dissertations and Theses (Open Access)

Lynch Syndrome (LS) is the most common inherited colorectal cancer (CRC) syndrome. It constitutes the perfect model to understand DNA mismatch repair deficient (MMRd) carcinogenesis, which underlies 15% of early-stage CRC. LS patients develop MMRd tumors with high loads of shared neoantigens (neoAgs), which are recognized by the immune system. Previous research has concentrated on discovering neoAgs and their potential as targets for vaccines in LS patients. However, these studies have primarily identified shared neoAgs from cancers, lacking detailed information on targetable neoAgs present in precancerous lesions. Understanding this landscape of pre-cancer derived neoAgs is crucial for intercepting cancer development …

Uncovering Capillary Endothelial Cells Response During Lung Injury-Repair, Celine Shuet Lin Kong

Dissertations and Theses (Open Access)

Once thought to be a homogenous population, capillary endothelial cells (ECs) have embodied organotypic specialization and heterogenous properties, both during homeostasis and tissue injury. In the lung, capillary ECs consist of two distinct populations, CAP1 and CAP2s; how each population responds to diverse tissue injury is incompletely understood. In this thesis, I report the induction and function of a truncated isoform of Ntrk2, Ntrk2^-tk (lacking the tyrosine kinase domain) in multiple injury models. Using a combinatorial approach of single-cell multiome, mouse genetics and viral infection models, I found that Ntrk2^-tk is broadly induced in CAP1s after the initial …

Investigating The Demise Of East Bank Ridge Through Local Fishery Perceptions And Fish Population Metrics, Marissa R. Lamb

Theses and Dissertations

East Bank Ridge is a natural reef formation located 18 nautical miles off the coast of South Padre Island, covering an area of over 80 km2, with the southern portion extending into Mexico. There is no previous record of the structural composition or fish community present at the site. The ridge was a well-known fishing site in the local community over the past decades but catch rates have decreased substantially according to personal accounts from local anglers. In this study, areas of reef habitat were identified, and fish presence and abundance metrics were determined at East Bank Ridge. These measures …

Investigating Autonomous Ground Vehicles For Weed Elimination, Abraham Mitchell

Computer Science and Computer Engineering Undergraduate Honors Theses

The management of weeds in crop fields is a continuous agricultural problem. The use of herbicides is the most common solution, but herbicidal resistance decreases effectiveness, and the use of herbicides has been found to have severe adverse effects on human health and the environment. The use of autonomous drone systems for weed elimination is an emerging solution, but challenges in GPS-based localization and navigation can impact the effectiveness of these systems. The goal of this thesis is to evaluate techniques for minimizing localization errors of drones as they attempt to eliminate weeds. A simulation environment was created to model …

Ecological Dynamics Of Cenozoic North American Mammals, Alexandria Brandis Shupinski

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding how community structure is affected by various ecological, climatic, and environmental changes is a long-standing goal in ecology. However, disentangling human impacts from these other variables is difficult when using short modern timescales and anthropogenically altered communities. The fossil record provides a means to investigate community structure dynamics across critical intervals, excluding humans as a variable. Here, in three studies, I assess changes in North American mammal paleocommunity structure across the last 66 million years (Ma) at multiple temporal and spatial scales. In Chapter 1, I identify changes in mammal functional diversity (FD) locally and continentally, using three independent …

Investigating Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome Using Mitotalens, Megan Gusso

Honors Program: Senior Projects (Public)

This lab project explores the complexity of the mitochondrial genome by investigating large genomic repeats and their effect on flowering plants’ reproduction and viability. Mitochondria are the site of cellular respiration to create energy and contain their own DNA. This is counterintuitive because the byproducts of cellular respiration impose a threat of mutation for the stored DNA. The mitochondrial genome differs in size between animals and plants. Plant mitochondrial DNA is larger due to the presence of large, noncoding repeated sequences, and a large amount of DNA of unknown function. These sequences undergo high levels of recombination and rearrangement. The …

Irt-Based Irrigation Scheduling And Dry Bean Crop Water Productivity Under Different Irrigation Scenarios In The Nebraska High Plains, Angie Gradiz

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Irrigation plays a critical role for crop production in western Nebraska of the United States, where crop evapotranspiration often exceeds in-season precipitation. This study investigated the impacts of different irrigation treatments on yield and key physiological parameters of dry edible beans grown in semi-arid western Nebraska during the 2022-2023 growing seasons. Seven irrigation levels (0%, 25%, 50%, 75%, 100%, 125%, 150%) of the fully irrigated treatment (FIT), were applied using a Variable Rate Irrigation (VRI) Linear-Move Sprinkler System. Additionally, an eighth treatment was scheduled based on measurement from infrared radiometry thermometers (IRT) to evaluate the performance of the two-source energy …

Toxicological Effects And Treatment Of Accidental Ingestion Of Simparica Trio® In Felis Catus: An Investigative Review, Aussia M. Stander

Honors Program: Senior Projects (Public)

Simparica TRIO^®, a canine endectocide manufactured by Zoetis, contains sarolaner, moxidectin, and pyrantel pamoate salt in an orally administered chewable tablet. These compounds are all toxic to both cats and dogs in sufficient amounts. As Simparica TRIO^® rises in popularity among pet owners, Felis catus (felines, cats) in multi-pet households (households with both cats and dogs) are at increased risk of overdosage and toxicosis from accidental ingestion of Simparica TRIO^® due to their smaller weight.

Simparica TRIO^® is not for feline use and little literature exists on the symptoms of and treatment for accidental ingestion …

Effects Of Dissolved Oxygen And Ph On Eastern Oyster (Crassostrea Virginica) Growth, Karina D. Ledezma

Honors Theses

Coastal ecosystems, known for their abundant natural resources and role in environmental processes, are facing challenges posed by climate change, pollution and anthropogenic activities. Among these challenges is the expansion of hypoxic zones, characterized by low dissolved oxygen (DO) levels, which poses a significant threat to benthic organisms and oyster fisheries. This research investigates the interaction between hypoxia and pH dynamics, intensified by freshwater input, and the biomineralization of Eastern oysters (Crassostrea virginica). To explore these dynamics, experimental trials were conducted in 24 tanks, simulating normoxic, hypoxic, and fresh hypoxic conditions, to assess the impact of hypoxia on …

Plant Model Of Α-Synucleinopathy: Expression Of Α-Synuclein A53t Variant In Hairy Root Cultures Leads To Proteostatic Stress And Dysregulation Of Iron Metabolism, Jasmina Kurepa, Kristen A. Bruce, Greg A. Gerhardt, Jan A. Smalle

Neurology Faculty Publications

Synucleinopathies, typified by Parkinson’s disease (PD), entail the accumulation of α- synuclein (αSyn) aggregates in nerve cells. Various αSyn mutants, including the αSyn A53T variant linked to early-onset PD, increase the propensity for αSyn aggregate formation. In addition to disrupting protein homeostasis and inducing proteostatic stress, the aggregation of αSyn in PD is associated with an imbalance in iron metabolism, which increases the generation of reactive oxygen species and causes oxidative stress. This study explored the impact of αSyn A53T expression in transgenic hairy roots of four medicinal plants (Lobelia cardinalis, Artemisia annua, Salvia miltiorrhiza, and Polygonum multiflorum). In all …

Evaluation Of Critical Weed-Free Period For Three Sweetpotato (Ipomoea Batatas) Cultivars, Emmanuel G. Cooper, Stephen L. Meyers, Jeanine Arana, Katherine Jennings, Ashley Adair, Kevin D. Gibson, William G. Johnson

Department of Horticulture & Landscape Architecture Faculty Publications

Sweetpotato [Ipomoea batatas (L.) Lam.] is a staple crop that provides nutritional benefits to humans globally, but it is subject to yield loss when competing with weeds, especially during the early stage of establishment. Yield loss can vary widely based on the cultivar, production environment, weed species, and management techniques. To address this challenge, we conducted field research at the Samuel G. Meigs Horticulture Research Farm, Lafayette, IN, and at the Southwest Purdue Agricultural Center, Vincennes, IN, in 2022 to determine the effect of sweetpotato cultivar on the critical weed-free period. The experiment was a split-plot design, with weed-free interval …

Using Additional Measures Of Handgrip Strength To Predict Aerobic Capacity In Wheelchair Users, Eric Naugle

All NMU Master's Theses

Manual wheelchair users have been shown to have low physical capacity and impaired ability to perform activities of daily living. Current tests and protocols for assessing physical capacity in wheelchair users have specific boundaries. Measuring handgrip strength (HGS) with electronic handgrip dynamometers has been shown to be a reliable measure of muscle function and a convenient assessment of overall muscle strength. This study aimed to determine the correlation of maximal HGS, HGS rate of force development, HGS fatigability, HGS isometric control, and HGS asymmetry to a six-minute wheelchair propulsion (6MPT) test in ambulatory young adults. Thirty-four recreationally active ambulatory individuals …

Evaluating Embryo Survival Of Surrogate Fish Species Using Multiple Incubators: Continuing Efforts Towards Reintroducing Arctic Grayling (Thymallus Arcticus) Into Lower Michigan Streams, Joshua Mutchler

All NMU Master's Theses

Arctic Grayling Thymallus arcticus have been successfully reintroduced to the Upper Missouri River Basin of Montana using Remote Site Incubators (RSI). Widespread use of RSIs as part of reintroduction efforts in Michigan is challenged by low gradient streams, which limit deployment success. In this study, I evaluated the utility of an alternative instream rearing device, the Floating Basket Incubator (FBI), by directly comparing the survival of Rainbow Trout Oncorhynchus mykiss, Brook Trout Salvelinus fontinalis, and Walleye Sander vitreus (as Arctic Grayling surrogates) between RSI and FBI in stream and hatchery environments. In addition, I assessed how abiotic and …

Sex Differences In Tibial Compression During Load Carriage Running On Challenging Surfaces, Eric Brian Francis

Boise State University Theses and Dissertations

Introduction: Tibial stress fracture is a common and destructive musculoskeletal issue for military, particularly female, personnel. Common military tasks, such as walking and running with body borne load, reportedly lead to lower limb biomechanics that elevate tibial stress fracture risk. Yet, it is unknown if similar increases are evident when running with heavy load – particularly over varied terrain personnel commonly navigate during military exercises. Purpose: This study sought to quantify vertical ground reaction force (GRF) and tibial compression while participants ran with body borne load over challenging surfaces, and determined whether they differed between sexes. Hypothesis: Participants will exhibit …

A Journey To The Center Of The Cell: The Characterization Of Novel Non-Canonical Mechanisms Of Notch Signaling, Tana Ranae Gazdik

Boise State University Theses and Dissertations

Cellular signaling pathways are the main method of communication between cells throughout the body. Of the many complex signaling pathways, the Notch signaling pathway is an ancient, highly conserved pathway critical to most animal life. This pathway is not only able to respond to juxtracrine signals from neighboring cells, but also acts as a microenvironment sensor, allowing cells to react to a variety of situations. Thus, understanding the mechanisms behind the regulation of Notch signaling is crucial to improving our basic understanding of Notch in cell biology and development. This dissertation introduces two previously undescribed non-canonical regulatory mechanisms of the …

Biological And Environmental Factors Associated With The Autumn Migration Timing Of North American Raptors, Nora R. Honkomp

Boise State University Theses and Dissertations

Migration is an energetically expensive and risky event in the life cycles of many organisms, and migration impacts survival and reproductive success through direct and carry-over effects. The timing of migration departure from the summer breeding grounds is important for determining the probability of survival and physical condition upon arrival at wintering sites. Many internal and external factors contribute to departure timing. It is important to study these factors across species, populations, and individuals to determine which traits and conditions are most important for shaping departure timing and its variability. Improving our understanding of the factors that affect organisms’ phenological …

Learning To Roll: Characterizing Infant Rolling With Biomechanics, Danielle N. Siegel

Boise State University Theses and Dissertations

Motor skills, like rolling, are an important part of an infant’s cognitive and motor development by facilitating interactions with others and their surrounding environment. Additionally, an infant’s musculoskeletal and motor development is largely affected by their environment and in the U.S., infants spend a majority of their time in commercial infant products, including those at an incline. The biomechanical impacts of infants spending time at an incline is an understudied area, and almost 12,000 emergency room visits occur each year due to injuries involving commercial infant products. Therefore, the overall objective of this study was to understand how an infant’s …

Nesting In Urban Areas Affects American Kestrel Home Range Size And Adult Female Health, Jessica Michele Taylor

Boise State University Theses and Dissertations

Urbanization is happening at an unprecedented rate, and wildlife must adapt or face population declines. American Kestrels inhabit urban areas, however, whether human-dominated landscapes impact kestrel space-use, survival and reproductive success is largely unknown. We monitored American Kestrel nest boxes along a gradient of urbanization, measured using impervious surface area (ISA), and deployed GPS tags on breeding adults to examine the relationship between urbanization, home range size, and adult and nestling health. Male kestrel home ranges were 9.14 times larger than female home ranges, and home range size decreased as ISA increased for both sexes (β = -1.96, SE = …