Life Sciences Commons^™

Phytoplankton Community Dynamics In The Northern California Current System, Kristin Forgrave

Student Research Symposium

The Northern California Current system is a productive coastal ecosystem that encompasses a variety of temporal and spatial features. The photosynthetic microbial community plays a crucial role in supporting the rich ecosystem and economically important fisheries. My research integrates data across distance and two years to investigate the community composition of two major phytoplankton groups in this system: picocyanobacteria and picoeukaryotes. The abundances and sizes of the phytoplankton were measured using flow cytometry. Picoeukaryotes were found to be present at similar concentrations in both summer and winter. Abundances of both cell types were comparable in the winter, but picocyanobacteria were …

Systematic Comparison Of Propensity Score Matching And Structural Causal Modeling For Clinical Applications, With A Case Study Of Albumin Treatment For Acute Kidney Injury (Aki) Patients: Propensity Score Matching Vs. Structural Causal Models, Layaal Khellah

Student Research Symposium

Introduction:

Propensity Score Matching (PSM) and Structural Causal Models (SCMs) are key approaches in causal inference for treatment evaluation and clinical decisions, both rooted in the potential outcome framework but differing in their foundations—PSM follows Rubin's Causal Model, while SCMs adhere to the Structural Theory of Causation.

Methods:

Our study compares PSM and SCMs in clinical contexts, focusing on albumin as an AKI treatment. We examine each method's steps, highlighting differences. Using data from AKI patients with cirrhosis, we assess albumin’s treatment efficacy with both PSM and SCMs, offering insights into their performance in estimating causal effects..

Results:

PSM and …

Understanding The Importance Of Social Emotional Learning In Children And The Link Between Mental Health, Lynette Marie Lubiak

Student Research Symposium

I will examine how very important it is to society to teach structured Social Emotional Learning (SEL) programs to children in school. It can bring mental wellbeing and the ability to have healthy relationships, non addictive behaviors and being able to emotionally regulate themselves. Since we have pushed on only academia and not SEL for our children, mental illness has skyrocketed. This problem started after the SEL part of school was taken out of schools and then the division between rich and poor became wider and wider as time went on. If we were to bring back SEL into school, …

Fireflies At Rpa Natural Area Final Report, Olivia H. Bush, Sydney M. Shea, Sofia J. Gordon, Nick S. Wiebke

Final Reports in ENST 411: Environmental Community Projects

Our group worked alongside community partners Shaunna Barnhart and Jim Dunn during the Spring 2024 semester to assist with their annual event Fireflies at RPA Natural Area. RPA Natural Area is a local park in South Williamsport, Pennsylvania that was recently acquired by Southside Recreational Authority. Since taking over the park, the organization has begun hosting an event each July to educate the general public about firefly biology and conservation by showing a film and guiding attendees through a firefly tour through the park. Our goals for this project included creating physical educational materials to be used at the park …

Stepping Towards Sustainability: Analyzing Walkability In Urban Environments, Elli Lewis

Student Theses 2015-Present

This paper examines path walkability in the urban environment and its various impact levels on different neighborhoods. One of the most accessible forms of sustainability is walking, as it produces zero carbon emissions and can harvest a greater sense of care for the human environment. In a growing urbanized world, providing citizens easy access to clean and safe sidewalks, parks, grocery stores, local restaurants, and religious organizations is essential. Accessible walking routes are vital in large, urban cities. The first chapter analyzes the need for walkable cities concerning ecosystem services and their functions. A brief history of New York’s urban …

Reef Ecosystem Conservation For The Hawaiian Islands: The Impacts Of Ocean Warming And Acidification On Benthic Reef Populations, Samantha Roberts

Student Theses 2015-Present

This paper explores the anthropogenic effects of rapid climate change and ocean acidification on benthic coral populations along the Hawaiian archipelago. Moreover, the reliance of Hawaiian coastal communities on coral reefs for sustenance and economic prosperity is evaluated to determine the importance of reef ecosystem conservation for the prosperity of Hawaii. Chapter 1 introduces the importance of coral and supplies quantitative data on declining coral populations traditional to Hawaii, as a result of ocean warming and acidification. Empirical data from prior, prominent studies, combined with my own, will be discussed. Chapter 2 outlines the ecology of Hawaiian reef ecosystems, the …

Ancient Wisdom, Modern Prosperity: Harnessing Traditional Ecological Knowledge To Revitalize Australia's Economy, Environment, And Human Wellbeing, Annabelle L. Baulch

Student Theses 2015-Present

This paper explores the traditional knowledge of Australia’s Indigenous people and how it can improve Australia's environment, health, and economic prosperity to shape a more sustainable future. Indigenous Australians managed the land for thousands of years; however, being forced off the land following European colonization resulted in terrible cultural, social, and environmental disruption for Aboriginal Australians and made conservation efforts difficult. Wildfires, imported species, mining, and agriculture is steadily destroying the Australian ecosystem, contributing to climate change, species extinction, and gaps in our cultural and ancestral knowledge. Chapter One overviews Australia's environmental issues; it uses quantitative data to explore the …

Socioeconomic Constraints On Low-Income Individuals' Perceptions Toward Food Safety, Uyen Thuy Xuan Phan, Cuc Thuy Thi Tran, Nguyet Minh Thi Nguyen

Food Science Faculty Articles and Research

Food safety has emerged as a critical concern in Vietnam, posing a significant threat to public health due to the prevalence of unsafe foods in the market. Among those most vulnerable to contaminated foods, low-income individuals are significantly affected. This study aimed to investigate the perceptions of food safety among this demographic, utilizing qualitative and quantitative approaches, including focus groups, survey questionnaires, and behavioral experiments using steamed bun as a stimulus. Participants were generally from low-income classes with certain variations in household monthly income. Results from focus groups and the survey highlighted that the factors influencing food purchases included perception …

Heavy Steers And Heifers Run At Low Stocking Rates Enhance Drought Resilience In A Pastoral Region Of Australia, Christophe D'Abbadie, Ross Kingwell, Philip E. Vercoe, Bradley Plunkett, Alan Peggs

Animal production and livestock research articles

CONTEXT

The semi-arid mulga lands of the Southern Rangelands of Western Australia experience frequent droughts. Maintaining a profitable animal grazing enterprise whilst preserving rangeland condition requires selecting an appropriate stocking rate and herd structure.

OBJECTIVE

We construct and apply bioeconomic models of beef enterprises for the semi-arid Southern Rangelands of Western Australia, to assess the profitability and relative riskiness of three different livestock production systems and three different stocking rates when exposed to different frequencies of drought.

METHODS

We construct bio-economic models of herd structures that separately focus on producing either: (i) 200 kg heifers and 210 kg steers (ii) …

Climate Change And Environmental Crises In Coastal Cities: Charleston Vs New York City, Nolan Rodriguez

Student Theses 2015-Present

This paper addresses the increasing vulnerability that coastal communities face regarding climate crises and rising sea levels. Specifically, this paper investigates the environmental crises facing Charleston, South Carolina, and New York City. The geographical location of these cities places a more severe threat upon their environment, as opposed to urban collectives removed from the immediate effect of rising sea levels. A cross-examination of politics and economics is discussed in order to determine the causal relationship of each city’s engagement with its surrounding environment. This paper examines how each city is affected by climate change, what measures are in place to …

The North Central Extension Risk Management Education Center, Part I: Helps Producers Manage Risks, Christine Lockert, Sheila Aikanathan Johnson, Bradley D. Lubben

Cornhusker Economics

Established in 2001, the North Central Extension Risk Management Education Center (NCERMEC) has a long history of supporting risk management education programs serving producers in the region. Located at the University of Nebraska-Lincoln Agricultural Economics Department, the Center is one of four regional Extension Risk Management Education (ERME) Centers located throughout the country. ERME is funded through USDA’s National Institute of Food and Agriculture (NIFA) and was authorized by the Agricultural Risk Protection Act of 2000. The fundamental purpose of the ERME program is to educate producers to manage risk and position their farm or ranch for future growth and …

Beyond The Tap: The Flint, Michigan Water Crisis As An Issue Of Environmental Racism, Vincent J. Vitanza

Student Theses 2015-Present

The following paper provides insight into the Flint, Michigan water crisis of 2014 through the lens of environmental racism. The Flint, Michigan water crisis saw the contamination of the municipal water supply of the town of Flint as a result of the decision to switch the main water source from Lake Huron to the Flint River as a temporary measure as the town of Flint switched to the Karegnondi Water Authority contract to supply their municipal water. Flint is a majority minority community, and the facets of race and socioeconomic status played a significant role in how the crisis was …

Immunopathogenesis Of Post-Infectious Hydrocephalus, Sascha Powers Bernier

Honors Scholar Theses

Hydrocephalus is characterized by the abnormal accumulation of cerebrospinal fluid (CSF) within the brain ventricles. In post-infectious hydrocephalus (PIH) cases, the condition presents challenges in understanding the immune response. PIH is a complex condition, often persisting after the initial infection is treated and thus requiring a deeper understanding of the immune mechanisms involved in its development. This thesis will explore the immunopathogenesis of PIH, elucidating the relationship between the immune response and neurological complications that would succeed infection. The immune response of PIH includes a series of events, beginning with the activation of immune cells and finishing with the release …

The Influence Of Subject Effort On Measurement Variability In Low-Stakes Tests Of Endurance Performance, Noah Wilfong

Masters Theses, 2020-current

Purpose: This study examined the effects of self-reported effort ratings on time trial performance variability in cyclists. Methods: Seven cyclists completed a preliminary trial and 3 identical exercise trials. Exercise trials consisted of 15 min of constant load cycling (5 min at 50% W_max, 10 min at 70% W_max), followed immediately by a 20-km self-paced time trial (TT). Performance was assessed as the time to complete the 20-km TT. Immediately following the exercise trial, participants completed two self-reported measures of expended effort (i.e., Student Opinion Subscale and Effort Thermometer scale) to ascertain their perceived effort during …

Adapting And Evaluating A Theory-Driven, Non-Pharmacological Intervention To Self-Manage Pain, Hulin Wu, Paul J Christo, Lorraine S Evangelista

Faculty, Staff and Student Publications

BACKGROUND: The existing literature has limited detail on theory-driven interventions, particularly in pain studies. We adapted Bandura's self-efficacy framework toward a theory-driven, non-pharmacological intervention using auricular point acupressure (APA) and evaluated participants' perceptions of this intervention on their pain self-management. APA is a non-invasive modality based on auricular acupuncture principles.

METHODS: We mapped our study intervention components according to Bandura's key sources of self-efficacy (performance accomplishments, vicarious experience, verbal persuasion, and emotional arousal) to facilitate the self-management of pain. Through a qualitative study design, we conducted virtual interviews at one and three months after a 4-week APA intervention among 23 …

Role Of Integrase-Pp2a Interaction In Human T-Cell Leukemia Virus Type-1 Replication And Pathogenesis, Shayna Turbin

Senior Honors Papers / Undergraduate Theses

Human T-cell leukemia virus type-1 (HTLV-1) is an oncogenic retrovirus that causes multiple disorders, including adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 retroviral integrase binds to the regulatory B’56γ subunit of the host cell Protein Phosphatase 2A (PP2A). Integrase contains a highly conserved LxxIxE motif that is essential for binding, which increases integration efficiency and facilitates HTLV-1 hijack of host cell machinery. We aim to understand how mutations introduced in the highly conserved binding site can affect viral particle production and infectivity. We transfected 729B human lymphoblastoid cells and 293T cells with mutant and wildtype virus. Mutations L213A, …

Antimicrobial Mechanisms Of Human Lipocalin-2, Lindsey Steinberg

Arts & Sciences Graduate Student Theses and Dissertations

Lipocalin-2 (Lcn2, also known as siderocalin or neutrophil gelatinase-associated lipocalin) is an innate immune protein released by neutrophils and epithelial cells at sites of infection. It is understood to limit bacterial growth by restricting access to iron, an essential nutrient, but it does not avidly bind iron in isolation. At least two iron restricting Lcn2 mechanisms are described in the literature. In the prototypical mechanism, Lcn2 binds and prevents uptake of enterobactin, a high affinity iron chelator secreted by E. coli and closely related Enterobacterales. Sequestration of this bacterial siderophore prevents nutritional iron delivery to these pathogens. However, the narrow …

Tracking And Mitigating Imprint Erasure During Human Pluripotent State Transitions, Laura A. Fischer

Arts & Sciences Graduate Student Theses and Dissertations

Early human embryogenesis is difficult to study due to practical and ethical limitations, but the successful culture of “naïve” human pluripotent stem cells (hPSCs) has expanded our ability to model cells akin to the pre-implantation epiblast. Naïve hPSCs are readily obtained through in vitro resetting of “primed” hPSCs, cells which model the post-implantation epiblast. Naïve hPSCs offer the advantages of modeling the pre-implantation transcriptome and epigenome, the ability to form embryonic and extraembryonic tissues, and the capacity to self-organize into integrated human embryo models. However, a persistent drawback of naïve hPSCs is the loss of parent-specific epigenetic marks, called imprints. …

Evaluating Pathological Tau Spread In Early Alzheimer Disease: Developing A Metric For Tau Positron Emission Tomography Quantifying Tau Spatial Spread, Stephanie Sierra Doering

Arts & Sciences Graduate Student Theses and Dissertations

The overall goal of this dissertation is to gain a better understanding of how pathological tau burden and tau spread are related at early stages of Alzheimer Disease, and whether each tau component is uniquely informative to key elements of AD progression. A spatiotemporal pattern of tau pathological progression is observed in AD and is highly correlated to regional neurodegeneration and domain-specific cognitive impairment. This progression is reflective of two biological processes: the aggregation of tau neurofibrillary tangles in early brain regions (tau burden) as well as the transmission of pathological tau to additional brain regions (tau spread). Positron emission …

Understanding Mechanisms Of Local Adaptation In White Clover (Trifolium Repens L.): Insights From A Phenotypic Cline And Population Genomics, Wen-Hsi Kuo

Arts & Sciences Graduate Student Theses and Dissertations

Rapid global climate change threatens food production and natural ecosystems. Knowledge of the mechanisms by which species adapt to local climates can inform future agriculture and conservation efforts and help to ensure sustainability and resilience of both cultivated and wild species. The overarching goal of this dissertation is to investigate the broad range of adaptive traits and their underlying genetics in wild populations of a widely distributed and polyploid forage plant, white clover. White clover is an ideal system because of its genetic diversity, widespread distribution, and its ability adapt to various environmental stresses through mechanisms that include its well-studied …

Microbial Community Composition And Assembly In Diverse Environmental Systems, Rhiannon Celeste Vargas

Arts & Sciences Graduate Student Theses and Dissertations

Analyzing microbial community responses across systems, particularly in coordinated ways, is critical to informing meaningful community ecology patterns. In Chapter 1, I provide historical contexts to the field of microbial community ecology. In Chapter 2 I have investigated a specific opportunistic pathogen, Staphyloccocus pseudintermedius, by utilizing whole genome sequencing to understand the genomic architecture that contributes to the community structures of S. pseudintermedius found in different environmental niches. We found that unlike other Staphylococcal pathogens, S. pseudintermedius niches are indistinguishable by core and accessory gene architecture. Rather, niches are resolved through the encoding of antimicrobial resistances such as those isolates …

Functions Of Cdc1 In Mediating Cd4+ Help For Cd8+ T Cells, Renee Wu

Arts & Sciences Graduate Student Theses and Dissertations

Classical dendritic cells (cDCs) are specialized antigen presenting cells that comprise distinct subsets distinguished by transcriptional developmental differences and in the types of immune responses they initiate. Classical type 1 dendritic cells (cDC1) are thought to perform antigen cross-presentation, which is required to prime CD8 T cells, whereas cDC2 have been suggested to be specialized for priming CD4 T cells. CD4 T cell help for CD8 T cell responses has been proposed to be mediated by CD40-dependent licensing of antigen-presenting cells. cDC1 have been suggested as the target of CD4 T cell help on the basis of in vitro analysis …

Crossing The Ocean: The Importance Of Gene Flow In Island Evolution, Ethan F. Gyllenhaal

Biology ETDs

Islands have long been a critical arena for the development of ecological and evolutionary theory, particular due to persistent open-water gaps between them. In this dissertation I demonstrates how these caps are permeable, which can be leveraged to understand evolution on islands and more broadly. The first chapter develops this connection between ongoing, overwater gene flow and island biogeographic theory, and tests it on an archipelago species. The second take a three-pronged approach in demonstrating how over-water gene flow can bias phylogenetic inference, and how many phylogeographic metrics in islands are likely shaped by long-term gene flow. The final two …

Response To Lao, Guan, Wang, Et Al, Casey Crump, Pär Stattin, James D Brooks, Jan Sundquist, Alexis C Edwards, Weiva Sieh

Faculty, Staff and Student Publications

No abstract provided.

Correction: Horne Et Al White Matter Correlates Of Domain-Specific Working Memory, Autumn Horne, Junhua Ding, Tatiana T Schnur, Randi C Martin

Faculty, Staff and Student Publications

In the original publication [...].

Exome Sequencing Implicates Ancestry-Related Mendelian Variation At Syne1 In Childhood-Onset Essential Hypertension, Ian Copeland, Edmond Wonkam-Tingang, Monesha Gupta-Malhotra, S Shahrukh Hashmi, Yixing Han, Nancy J Hall, Natasha Lie, Dan Liu, Jun Xu, Jill Rosenfeld, Aparna Haldipur, Zelene Desire, Zeynep H Coban-Akdemir, Daryl A Scott, Qing Li, Hsiao-Tuan Chao, Ana M Zaske, James R Lupski, Dianna M Milewicz, Sanjay Shete, Jennifer E Posey, Neil A Hanchard

Faculty, Staff and Student Publications

Childhood-onset essential hypertension (COEH) is an uncommon form of hypertension that manifests in childhood or adolescence and, in the United States, disproportionately affects children of African ancestry. The etiology of COEH is unknown, but its childhood onset, low prevalence, high heritability, and skewed ancestral demography suggest the potential to identify rare genetic variation segregating in a Mendelian manner among affected individuals and thereby implicate genes important to disease pathogenesis. However, no COEH genes have been reported to date. Here, we identify recessive segregation of rare and putatively damaging missense variation in the spectrin domain of spectrin repeat containing nuclear envelope …

Autism Risk Gene Cul3 Alters Neuronal Morphology Via Caspase-3 Activity In Mouse Hippocampal Neurons, Qiang-Qiang Xia, Anju Singh, Jing Wang, Zhong Xin Xuan, Jeffrey D. Singer, Craig M. Powell

Biology Faculty Publications and Presentations

Autism Spectrum Disorders (ASDs) are neurodevelopmental disorders (NDDs) in which children display differences in social interaction/communication and repetitive stereotyped behaviors along with variable associated features. Cul3, a gene linked to ASD, encodes CUL3 (CULLIN-3), a protein that serves as a key component of a ubiquitin ligase complex with unclear function in neurons. Cul3 homozygous deletion in mice is embryonic lethal; thus, we examine the role of Cul3 deletion in early synapse development and neuronal morphology in hippocampal primary neuronal cultures. Homozygous deletion of Cul3 significantly decreased dendritic complexity and dendritic length, as well as axon formation. Synaptic spine density significantly …

Chronic Stress-Induced Neuroinflammation: Relevance Of Rodent Models To Human Disease., Abigail G White, Elias Elias, Andrea Orozco, Shivon A Robinson, Melissa T Manners

College of Science & Mathematics Departmental Research

The brain is the central organ of adaptation to stress because it perceives and determines threats that induce behavioral, physiological, and molecular responses. In humans, chronic stress manifests as an enduring consistent feeling of pressure and being overwhelmed for an extended duration. This can result in a persistent proinflammatory response in the peripheral and central nervous system (CNS), resulting in cellular, physiological, and behavioral effects. Compounding stressors may increase the risk of chronic-stress-induced inflammation, which can yield serious health consequences, including mental health disorders. This review summarizes the current knowledge surrounding the neuroinflammatory response in rodent models of chronic stress-a …

From Pasture To Pavement: Urban Expansion And Its Environmental Consequences In Perth, Anastasia Charelishvili

Student Theses 2015-Present

This thesis addresses the pressing issue of ecological problems of urban sprawl and its intricate impacts on urban health, with a particular focus on vulnerable communities in Perth, Australia. Chapter 1 presents the city's historical background and emphasizes the depletion of ecosystem services, underscoring the need for environmental justice. It also introduces the causes and effects of the sprawl in Perth and draws upon a diverse range of environmental problems created by suburbia, such as air pollution, biodiversity loss, water pollution, and runoff. As these threats translate into urban health declines, such as respiratory problems and increased healthcare issues, Chapter …

Researching Covid To Enhance Recovery (Recover) Pediatric Study Protocol: Rationale, Objectives And Design, Rachel S. Gross, Tanayott Thaweethai, Erika B. Rosenzweig, James Chan, Lori B. Chibnik, Mine S. Cicek, Amy J. Elliott, Valerie J. Flaherman, Andrea S. Foulkes, Margot Gage Witvliet, Richard Gallagher, Maria Laura Gennaro, Terry L. Jernigan, Elizabeth W. Karlson, Stuart D. Katz, Patricia A. Kinser, Lawrence C. Kleinman, Michelle F. Lamendola-Essel, Joshua D. Milner, Sindhu Mohandas, Praveen C. Mudumbi, Jane W. Newburger, Kyung E. Rhee, Amy L. Salisbury, Jessica N. Snowden, Cheryl R. Stein, Melissa S. Stockwell, Kelan G. Tantisira, Tamara Bradford, Et Al

School of Medicine Faculty Publications

Importance The prevalence, pathophysiology, and long-term outcomes of COVID-19 (post-acute sequelae of SARS-CoV-2 [PASC] or “Long COVID”) in children and young adults remain unknown. Studies must address the urgent need to define PASC, its mechanisms, and potential treatment targets in children and young adults. Observations We describe the protocol for the Pediatric Observational Cohort Study of the NIH’s REsearching COVID to Enhance Recovery (RECOVER) Initiative. RECOVER-Pediatrics is an observational meta-cohort study of caregiver-child pairs (birth through 17 years) and young adults (18 through 25 years), recruited from more than 100 sites across the US. This report focuses on two of …