Life Sciences Commons^™

South Carolina Master Naturalist Program Evaluation: A Mixed Methods Approach, Susan T. Guynn, James H. Blake, Nathan Nemire, Joe Bible

Journal of Extension

The South Carolina Master Naturalist Program provides nature-based education to citizen volunteers who will promote environmental stewardship and is offered at six host sites across the state. We conducted a mixed-methods evaluation (the integration of qualitative and quantitative data) of the South Carolina Master Naturalist Program. Overall, the South Carolina Master Naturalist Program is achieving its mission but there were some differences between host sites. We identified where there were differences and why those differences may exist. We make recommendations to help strengthen the program across all host sites in the areas of program fidelity, delivery, and administration.

Beefsd: An Integrated And Intensive Extension Curriculum For Beginning Beef Cattle Producers, Krista A. Ehlert, Amanda D. Blair, Stacy Hadrick, Ken Olson

Journal of Extension

There is a strong need to increase the opportunities for and success of the next generation of beef cattle producers. South Dakota State University Extension provides a 2-year educational program called “beefSD;” the purpose is to provide producers with a holistic view of the beef industry, an appreciation for utilizing adaptive management to respond to challenges and opportunities, and skills to assess and manage factors that influence overall operation sustainability. The curriculum has four major components: 1) in person workshops, case studies, interactive webinars, and homework 2) out-of-state learning experiences, 3) ranch to rail program, and 4) networking and mentoring.

Meals For All: An Examination Of The Effects Of Free School Meals On Middle Class Households, Ryan Elizabeth Comerford

Doctoral Dissertations and Projects

Universal meal programs were created to mitigate childhood food insecurity in low-income communities. It is well known that poverty and food insecurity go hand-in-hand and are often the basis for much academic research within the realm of childhood food insecurity. This study investigates the impact of universal meal programs on food insecurity from a different perspective, that of the middle class. As an under-researched area, the hope is to draw attention to the needs of communities that are often forgotten and may not qualify for social programs, in an effort to reform existing federal policy regarding Universal Meal programs. Utilizing …

Seasonal Activity Patterns And Home Range Sizes Of Wolves In The Human-Dominated Landscape Of Northeast Türkiye, J. David Blount, Austin M. Green, Mark Chynoweth, Kyle D. Kittelberger, Dário Hipólito, Katarzyna Bojarska, Emrah Çoban, Josip Kusak, Çağan H. Şekercioğlu

Wildland Resources Faculty Publications

Gray wolves Canis lupus comprise one of the most widely distributed carnivore species on the planet, but they face myriad environmental and anthropogenic pressures. Previous research suggests that wolves adjust their time- and space-use seasonally to mitigate risks from humans, conspecifics, and other predators while maximizing their hunting and reproductive success. With many populations of wolves resettling in areas with dense human populations, understanding how wolves may adjust their temporal and spatial patterns in these more human-dominated landscapes is of high conservation importance. Typically, human presence causes wolves to increase their nocturnality and home range size. Here, we look at …

Mediterranean Diet Information On Tiktok And Implications For Digital Health Promotion Research: Social Media Content Analysis, Margaret Raber, Haley Allen, Sophia Huang, Maria Vazquez, Echo Warner, Debbe Thompson

Children’s Nutrition Research Center Staff Publications

Background: The Mediterranean diet has been linked to reduced risk for several cardiometabolic diseases. The lack of a clear definition of the Mediterranean diet in the scientific literature and the documented proliferation of nutrition misinformation on the internet suggest the potential for confusion among consumers seeking web-based Mediterranean diet information.

Objective: We conducted a social media content analysis of information about the Mediterranean diet on the influential social media platform, TikTok, to examine public discourse about the diet and identify potential areas of misinformation. We then analyzed these findings in the context of health promotion to identify potential challenges and …

Sea Urchins Like Zinc Oxide Nanometric Mitigating Meloidogyne Incognita Infection In Eggplant, Amir Khan, Manar Fawzi Bani Mfarrej, Mohd Farhan Khan, Shahbaz Khan, Peiter Malan, Saurabh C. Saxena, Mohd Muddassir, Faheem Ahmad

All Works

This study focused on the nematicidal potential of sea urchin-like zinc oxide nanometrics (SUZN) against the root-knot nematode Meloidogyne incognita. XRD, SEM, UV-vis spectroscopy, TEM, and EDX have been utilized to characterize the SUZN. Various SUZN concentrations (150–750 ppm) were examined for their implications for M. incognita second-stage juveniles (J2s) and egg masses, including mortality as well as hatching inhibition. The largest J2s hatching inhibition (83.07%) was seen at 750 ppm SUZN after 48 hours of incubation, whereas the lowest hatching occurred at 150 ppm when compared to the control. SUZN illustrated increased nematicidal efficiency with increasing concentration and incubation …

Cornus Officinalis Extract Activates The Keap1/Nrf2 Pathway And Delays T1d Onset In The Nod Mouse, Justin D. Fletcher

USF Tampa Graduate Theses and Dissertations

Type 1 diabetes (T1D) is an autoimmune disorder that results in the destruction of the insulin producing pancreatic β cells. The only therapeutic option following T1D clinical onset is exogenous insulin supplementation. At present, there is no cure for T1D and attempted interventional therapy has been focused on modulating the immune system. Few attempts have been pursued at preserving β cell function and survival. Here we provide a novel approach using extract from the fruit of the Cornus officinalis (CO) tree to preserve β cell function through the prevention of β cell stress. CO has been used in traditional Chinese …

Editorial For "Parallel Cnn-Deep Learning Clinical-Imaging Signature For Assessing Pathologic Grade And Prognosis Of Soft Tissue Sarcoma Patients", Pegah Khosravi

Publications and Research

No abstract provided.

Microglial P2y6 Calcium Signaling Promotes Phagocytosis And Shapes Neuroimmune Responses In Epileptogenesis, Anthony D Umpierre, Bohan Li, Katayoun Ayasoufi, Whitney L Simon, Shunyi Zhao, Grace Thyen, Jiaying Zheng, Yue Liang, Dale B Bosco, Mark A Maynes, Zhaofa Wu, Xinzhu Yu, Jaeyun Sung, Aaron J Johnson, Yulong Li, Long-Jun Wu

Faculty, Staff and Student Publications

Microglial calcium signaling is rare in a baseline state but strongly engaged during early epilepsy development. The mechanism(s) governing microglial calcium signaling are not known. By developing an in vivo uridine diphosphate (UDP) fluorescent sensor, GRABUDP1.0, we discovered that UDP release is a conserved response to seizures and excitotoxicity across brain regions. UDP can signal through the microglial-enriched P2Y6 receptor to increase calcium activity during epileptogenesis. P2Y6 calcium activity is associated with lysosome biogenesis and enhanced production of NF-κB-related cytokines. In the hippocampus, knockout of the P2Y6 receptor prevents microglia from fully engulfing neurons. Attenuating microglial calcium signaling through calcium …

Keep Fingers On The Cpg Islands, Xing Zhang, Robert M Blumenthal, Xiaodong Cheng

Faculty, Staff and Student Publications

The post-genomic era has ushered in the extensive application of epigenetic editing tools, allowing for precise alterations of gene expression. The use of reprogrammable editors that carry transcriptional corepressors has significant potential for long-term epigenetic silencing for the treatment of human diseases. The ideal scenario involves precise targeting of a specific genomic location by a DNA-binding domain, ensuring there are no off-target effects and that the process yields no genetic remnants aside from specific epigenetic modifications (i.e., DNA methylation). A notable example is a recent study on the mouse

Challenges And Opportunities In Cancer Immunotherapy: A Society For Immunotherapy Of Cancer (Sitc) Strategic Vision, Leisha A Emens, Pedro J Romero, Ana Carrizosa Anderson, Tullia C Bruno, Christian M Capitini, James L Gulley, Avery D Posey, Ann W Silk, Jennifer A Wargo

Faculty, Staff and Student Publications

Cancer immunotherapy has flourished over the last 10-15 years, transforming the practice of oncology and providing long-term clinical benefit to some patients. During this time, three distinct classes of immune checkpoint inhibitors, chimeric antigen receptor-T cell therapies specific for two targets, and two distinct classes of bispecific T cell engagers, a vaccine, and an oncolytic virus have joined cytokines as a standard of cancer care. At the same time, scientific progress has delivered vast amounts of new knowledge. For example, advances in technologies such as single-cell sequencing and spatial transcriptomics have provided deep insights into the immunobiology of the tumor …

Neuroecological Links Of The Exposome And One Health, Agustin Ibanez, Lucia Melloni, Paweł Świeboda, William Hynes, Burcin Ikiz, Massamba Thioye, Bahar Güntekin, Jyoti Mishra, Mohamed Salama, Sarah Dunlop, Claudia Duran-Aniotz, Harris A Eyre

Faculty, Staff and Student Publications

This NeuroView assesses the interplay among exposome, One Health, and brain capital in health and disease. Physical and social exposomes affect brain health, and green brain skills are required for environmental health strategies. Ibanez et al. address current gaps and strategies needed in research, policy, and technology, offering a road map for stakeholders.

Culinary Nutrition Education To Division 3 Athletes, Jonathan Robert Poyourow

Doctoral Dissertations and Projects

This paper examines the impact of culinary nutrition education on the athletic potential of Division 3 athletes using culinary nutrition education. In the last five to ten years, the number of registered dietitians (RDs) working with collegiate athletes full-time in the United States has increased dramatically. However, even at large Division 1 universities that have these staff members, many student-athletes may still be at increased risk of nutrition-related problems that impact physical and academic performance. They may also include inappropriate macronutrient and micronutrient consumption. While a great deal of research has been conducted at Division 1 universities regarding sports nutrition …

How An Ijes Working Group Grappled With The Complexities Of Three Letters—Dei—With The Goal To Broaden Inclusion And Representation In Exercise Science Research, Dustin W. Davis, Matthew J. Garver, Jafra Thomas, James W. Navalta, Shannon Siegel, Joel D. Reece, Jill M. Maples

International Journal of Exercise Science

International Journal of Exercise Science 17(8): 852-860, 2024. A self-study of International Journal of Exercise Science (IJES) publications from 2008 through 2021 revealed a sex-data gap in participant and corresponding author representation. This finding prompted the creation of the IJES Working Group for Opportunity, Representation, and Diverse Perspectives. We are a collaborative team of authors, reviewers, and editors, spanning both rural and urban teaching- and research-focused institutions, who assembled regularly starting in September 2023. The goal was to contemplate potential challenges and opportunities around diversity, equity, and inclusion (DEI), elicit discourse, and broaden inclusion and representation in exercise science research. …

Bimanual Coordination In Individuals Post-Stroke: Constraints, Rehabilitation Approaches And Measures: Systematic Review, Yutong Liu, Eryk Przysucha, Taryn Klarner, Carlos Zerpa, Keshini Madara Marasinghe

International Journal of Exercise Science

International Journal of Exercise Science 17(3): 831-851, 2024. To couple or not to couple is a dilemma for the CNS when performing bimanual goal-directed actions. Numerous interacting individual and task-related constraints contribute to the issue of effective movement coordination, and their impact on the emerging actions must be inferred from valid methodologies. This is particularly important when examining coordination in individuals with stroke undergoing rehabilitation. The purpose of this review was to identify the different constraints that may impact inter-limb coupling, and the rehabilitation approaches implemented to enhance those actions. Also, the measures incorporated to examine the effects of rehabilitation …

Daily Eicosapentaenoic Acid Infusion In Iugr Fetal Lambs Reduced Systemic Inflammation, Increased Muscle Adrβ2 Content, And Improved Myoblast Function And Muscle Growth, Haley Beer, Taylor A. Lacey, Rachel L. Gibbs, Micah S. Most, Zena M. Hicks, Pablo C. Grijalva, Eileen S. Marks-Nelson, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth-restricted (IUGR) fetuses exhibit systemic inflammation that contributes to programmed deficits in myoblast function and muscle growth. Thus, we sought to determine if targeting fetal inflammation improves muscle growth outcomes. Heat stress-induced IUGR fetal lambs were infused with eicosapentaenoic acid (IUGR+EPA; n = 9) or saline (IUGR; n = 8) for 5 days during late gestation and compared to saline-infused controls (n = 11). Circulating eicosapentaenoic acid was 42% less (p < 0.05) for IUGR fetuses but was recovered in IUGR+EPA fetuses. The infusion did not improve placental function or fetal O2 but resolved the 67% greater (p < 0.05) circulating TNFα observed in IUGR fetuses. This improved myoblast function and muscle growth, as the 23% reduction (p < 0.05) in the ex vivo differentiation of IUGR myoblasts was resolved in IUGR+EPA myoblasts. Semitendinosus, longissimus dorsi, and flexor digitorum superficialis muscles were 24–39% lighter (p < 0.05) for IUGR but not for IUGR+EPA fetuses. Elevated (p < 0.05) IL6R and reduced (p < 0.05) β2 adrenoceptor content in IUGR muscle indicated enhanced inflammatory sensitivity and diminished β2 adrenergic sensitivity. Although IL6R remained elevated, β2 adrenoceptor deficits were resolved in IUGR+EPA muscle, demonstrating a unique underlying mechanism for muscle dysregulation. These findings show that fetal inflammation contributes to IUGR muscle growth deficits and thus may be an effective target for intervention.

Oregon State Rank Assessment For Black-Backed Woodpecker (Picoides Arcticus), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Black-backed woodpecker (Picoides arcticus) using NatureServe methodology, 2024.

The Indigenization Of Tobacco Farming And Its Challenges In Iringa District: 1960s–1980s, Emmanuel Kihongo

Journal of Humanities and Social Sciences

This paper is about the politics of indigenizing tobacco farming and the challenges associated with it during the post-colonial era. It examines the initiatives of government in facilitating the process, and how the bodies entrusted to ensure its implementation failed to achieve the intended goal. Tobacco farming in Africa and Tanzania has a complex history. Since this crop is essentially labour- and capital intensive, its beginning in colonial Tanganyika, and Iringa in particular, was dominated by settler farmers. The paper progressively narrates this history, especially on how it became a popular crop among indigenous smallholders in Iringa from 1961. It …

Why Trees Grow Where They Do - In Nh Forests, William B. Leak, Jane R. Riddle

UNH Cooperative Extension

No abstract provided.

Tissue‑ And Time‑Dependent Metabolite Profiles During Early Grain Development Under Normal And High Night‑Time Temperature Conditions, Nathan Abshire, Andrew L. Hauck, Harkamal Walia, Toshihiro Obata

Department of Agronomy and Horticulture: Faculty Publications

Background Wheat grain development in the first few days after pollination determines the number of endosperm cells that influence grain yield potential and is susceptible to various environmental conditions, including high night temperatures (HNTs). Flag leaves and seed-associated bracts (glumes, awn, palea, and lemma) provide nutrients to the developing seed. However, the specific metabolic roles of these tissues are uncertain, especially their dynamics at different developmental stages and the time in a day. Tissue- and time-dependent metabolite profiling may hint at the metabolic roles of tissues and the mechanisms of how HNTs affect daytime metabolic status in early grain development. …

Chromosome-Scale Assemblies And A Pangenome Reveal Ltr Repeat Dynamics As A Major Driver Of Genome Evolution In Chenopodium, Kate E. Jaggi

Theses and Dissertations

The genus Chenopodium, which comprises around 150 species globally, is characterized by its wide geographic distribution and ecological adaptability, particularly in temperate regions. Some species, such as Chenopodium quinoa and Chenopodium pallidicaule, have served as domesticated staple crops in the Andean region of South America for centuries, valued for their robust nutritional profile and adaptability to adverse environmental conditions. In contrast, wild Chenopodium species exhibit broad geographic distributions and diverse niche adaptations, functioning as reservoirs for beneficial traits, including disease resistance and climate hardiness, essential for enhancing domesticated crops through introgression and breeding efforts. However, taxonomic classification within the genus …

Operation Villagization And Its Implications For Wildlife Conservation In South-Western Serengeti, Tanzania: The Case Of Maswa Game Reserve, Ca. 1970s–1980s, Beny Nampesya, Hezron Kangalawe

Journal of Humanities and Social Sciences

Operation Villagization in Tanzania attracted the attention of many scholars, including historians, inside and outside the country. This is because it is one of the major policies in the history of Tanzania that affected many sectors. Despite the extensive scope of these studies, minimal attention has been devoted to exploring the connections between this operation and wildlife conservation. The current article uses the case of the Maswa Game Reserve (MGR) to examine the extent to which the operation affected wildlife resources conservation. In essence, it argues that poor implementation of the operation led to enormous encroachments in the MGR, which …

Altered Extracellular Matrix Correlates With An Immunosuppressive Tumor Microenvironment And Disease Progression In Younger Adults With Oral Cavity Squamous Cell Carcinoma, Leonard Estephan, Gaurav Kumar, Matthew Stewart, Raphael Banoub, Alban Linnenbach, Larry Harshyne, Ubaldo Martinez-Outshoorn, My Mahoney, Joseph Curry, Jennifer Johnson, Andrew South, Adam Luginbuhl

Kimmel Cancer Center Faculty Papers

INTRODUCTION: Oral cavity squamous cell carcinoma (OSCC) occurs most frequently in patients >60 years old with a history of tobacco and alcohol use. Epidemiological studies describe increased incidence of OSCC in younger adults (years). Despite its poor prognosis, knowledge of OSCC tumor microenvironment (TME) characteristics in younger adults is scarce and could help inform possible resistance to emerging treatment options.

METHODS: Patients with OSCC were evaluated using TCGA-HNSC (n=121) and a stage and subsite-matched institutional cohort (n=8) to identify differential gene expression focusing on the extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT) processes in younger (≤45 years) vs. older adults …

Oregon State Rank Assessment For Western Toad (Anaxyrus Boreas), Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Western toad (Anaxyrus boreas) using NatureServe methodology, 2024.

Oregon State Rank Assessment For Golden Eagle (Aquila Chrysaetos), Misty Nelson, Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Golden Eagle (Aquila chrysaetos) using NatureServe methodology, 2024.

From Waste To Wealth: Iron Oxide Doped Hydroxyapatite-Based Biosensor For The Colorimetric Detection Of Ascorbic Acid, Amir Badshah, Sadaf Noreen, Mohibullah Shah, Muhammad Asad, Riaz Ullah, Essam A. Ali, Jibran Iqbal, Wei Sun, Umar Nishan

All Works

Ascorbic acid plays a pivotal role in the human body. It maintains the robustness, enlargement, and elasticity of the collagen triple helix. However, the abnormal concentration of ascorbic acid causes various diseases, such as scurvy, cardiovascular diseases, gingival bleeding, urinary stones, diarrhea, stomach convulsions, etc. In the present work, an iron-doped hydroxyapatite (HAp@Fe2O3)-based biosensor was developed for the colorimetric detection of ascorbic acid based on a low-cost, biocompatible, and ubiquitous material. Due to the catalytic nature of HAp owing to the acidic and basic moieties within the structure, it was used as a template for HAp@Fe2O3 synthesis. This approach provides …

Oregon State Rank Assessment For Brandt's Cormorant (Phalacrocorax Penicillatus), Misty Nelson, Eleanor P. Gaines

Institute for Natural Resources Publications

Oregon state conservation status assessment for Brandt's cormorant (Phalacrocorax penicillatus) using NatureServe methodology, 2024.

Effects Of Exercise Or Metformin On Myokine Concentrations In Patients With Breast And Colorectal Cancer: A Phase Ii Multi-Centre Factorial Randomized Trial, Justin C. Brown, Guillaume Spielmann, Shengping Yang, Stephanie L.E. Compton, Lee W. Jones, Melinda L. Irwin, Jennifer A. Ligibel, Jeffrey A. Meyerhardt

School of Medicine Faculty Publications

Background: Physical activity and metformin pharmacotherapy are associated with improved clinical outcomes in breast and colorectal cancer survivors. Myokines are cytokines secreted from skeletal muscle that may mediate these associations. Methods: This hypothesis-generating analysis used biospecimens collected from a multi-centre 2 × 2 factorial randomized design of 116 patients with stage I–III breast and colorectal cancer who were randomized to 12 weeks of (1) aerobic exercise (moderate intensity titrated to 220 min/week); (2) metformin (850 mg daily for 2 weeks and then titrated to 850 mg twice per day); (3) aerobic exercise and metformin; or (4) control. Fourteen myokines were …

Photopolymer Material Durability And Safety In Holographic Diffusers For Visual Applications, Matthew Hellis, Alan Casey, Edoardo Splendi, Suzanne Martin, Matthew Sheehan, Kevin Murphy

Articles

This study introduces novel holographic diffuser applications employing acrylamide- or diacetone acrylamide-based photopolymers, patterned within the volume on a micron scale by a single-beam holographic recording process. These diffusers have previously been presented as potential treatments for amblyopia and diplopia. This work has now been extended to spectrometric analysis to determine their properties under broadband light. Diffusive elements with higher diffusion efficiencies exhibited a marginal reduction (< 5%) in diffusion efficiency across most of the visible spectrum. Given the intended application of these holographic diffusers, cytotoxicity assessments were also performed. This is significant as there is a difference in toxicity between the crystalline acrylamide (classified as a category 3 material) and diacetone acrylamide (classified as a category 4 material). The findings indicated substantially lower toxicity in holograms produced with diacetone acrylamide-based photopolymer. The accelerated ageing of both formulations of holographic diffusers indicated that the acrylamide-based holographic diffusers did not reduce efficacy in the 292 days of ageing. The diacetone acrylamide-based holographic diffusers exhibited reduced efficacy by day 182. Despite this, both formulations have been shown to perform for prolonged periods as the treatment modality would require. These results emphasise that holographic diffusers exhibit minimal spectral impact, and longevity on the scale of treatment regimes which are crucial considerations for their prospective use case as treatments for amblyopia and diplopia.

Neurogranin Modulates The Rate Of Association Between Calmodulin And Target Peptides, John A Putkey, Laurel Hoffman, Vladimir Berka, Xu Wang

Faculty, Staff and Student Publications

The best-known mode of action of calmodulin (CaM) is binding of Ca2+ to its N- and C-domains, followed by binding to target proteins. An underappreciated facet of this process is that CaM is typically bound to proteins at basal levels of free Ca2+, including the small, intrinsically disordered, neuronal IQ-motif proteins called PEP-19 and neurogranin (Ng). PEP-19 and Ng would not be effective competitive inhibitors of high-affinity Ca2+-dependent CaM targets at equilibrium because they bind to CaM with relatively low affinity, but they could influence the time course of CaM signaling by affecting the rate of association of CaM with …