Life Sciences Commons^™

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Outcomes Of The Emdataresource Cryo-Em Ligand Modeling Challenge, Catherine L Lawson, Andriy Kryshtafovych, Grigore D Pintilie, Stephen K Burley, Jiří Černý, Vincent B Chen, Paul Emsley, Alberto Gobbi, Andrzej Joachimiak, Sigrid Noreng, Michael G Prisant, Randy J Read, Jane S Richardson, Alexis L Rohou, Bohdan Schneider, Benjamin D Sellers, Chenghua Shao, Elizabeth Sourial, Chris I Williams, Christopher J Williams, Ying Yang, Venkat Abbaraju, Pavel V Afonine, Matthew L Baker, Paul S Bond, Tom L Blundell, Tom Burnley, Arthur Campbell, Renzhi Cao, Jianlin Cheng, Grzegorz Chojnowski, K D Cowtan, Frank Dimaio, Reza Esmaeeli, Nabin Giri, Helmut Grubmüller, Soon Wen Hoh, Jie Hou, Corey F Hryc, Carola Hunte, Maxim Igaev, Agnel P Joseph, Wei-Chun Kao, Daisuke Kihara, Dilip Kumar, Lijun Lang, Sean Lin, Sai R Maddhuri Venkata Subramaniya, Sumit Mittal, Arup Mondal, Nigel W Moriarty, Andrew Muenks, Garib N Murshudov, Robert A Nicholls, Mateusz Olek, Colin M Palmer, Alberto Perez, Emmi Pohjolainen, Karunakar R Pothula, Christopher N Rowley, Daipayan Sarkar, Luisa U Schäfer, Christopher J Schlicksup, Gunnar F Schröder, Mrinal Shekhar, Dong Si, Abhishek Singharoy, Oleg V Sobolev, Genki Terashi, Andrea C Vaiana, Sundeep C Vedithi, Jacob Verburgt, Xiao Wang, Rangana Warshamanage, Martyn D Winn, Simone Weyand, Keitaro Yamashita, Minglei Zhao, Michael F Schmid, Helen M Berman, Wah Chiu

Children’s Nutrition Research Center Staff Publications

The EMDataResource Ligand Model Challenge aimed to assess the reliability and reproducibility of modeling ligands bound to protein and protein-nucleic acid complexes in cryogenic electron microscopy (cryo-EM) maps determined at near-atomic (1.9-2.5 Å) resolution. Three published maps were selected as targets: Escherichia coli beta-galactosidase with inhibitor, SARS-CoV-2 virus RNA-dependent RNA polymerase with covalently bound nucleotide analog and SARS-CoV-2 virus ion channel ORF3a with bound lipid. Sixty-one models were submitted from 17 independent research groups, each with supporting workflow details. The quality of submitted ligand models and surrounding atoms were analyzed by visual inspection and quantification of local map quality, model-to-map …

Lactobacillus Rhamnosus Interactions With Foodborne Pathogens And Impact On Plant Productivity In A Model Hydroponic System, Esther Titilope Oginni

Theses and Dissertations

Food production is a global problem, and this has been further compounded with changes in weather patterns necessitating the need for a sustainable approach to production. Chapter 1 introduces the broad concept of the Control environment agriculture (CEA) and then narrows it doe to the hydroponic system its food safety risks. The CEA has been employed alongside conventional practices methods to improve meet the demand for food without placing pressure on the land available. Foodborne outbreaks have been recorded in the production medium and are therefore susceptible to contamination. In Chapter 2, the study focused on using Lactobacillus rhamnosus and …

Identifying And Training Deep Learning Neural Networks On Biomedical-Related Datasets, Alan E. Woessner, Usman Anjum, Hadi Salman, Jacob Lear, Jeffrey T. Turner, Ross Campbell, Laura Beaudry, Justin Zhan, Lawrence E. Cornett, Susan Gauch, Kyle P. Quinn

Computer Science and Computer Engineering Faculty Publications and Presentations

This manuscript describes the development of a resources module that is part of a learning platform named 'NIGMS Sandbox for Cloud-based Learning' https://github.com/NIGMS/NIGMS-Sandbox. The overall genesis of the Sandbox is described in the editorial NIGMS Sandbox at the beginning of this Supplement. This module delivers learning materials on implementing deep learning algorithms for biomedical image data in an interactive format that uses appropriate cloud resources for data access and analyses. Biomedical-related datasets are widely used in both research and clinical settings, but the ability for professionally trained clinicians and researchers to interpret datasets becomes difficult as the size and …

The Role Of Obesity In The Development Of Disordered Breathing, Sarah N. Framnes-Deboer

Master's Theses (2009 -)

Obesity is a complex disease that is defined by an excess amount of body fat. Obesity can have physical, neurological, and endocrine effects and detrimental effects on health. Some obese co-morbid conditions, such as type 2 diabetes have known mechanisms, while others, such as sleep disordered breathing do not. Moreover, there are common respiratory diseases associated with obesity, such as obstructive sleep apnea and obesity hypoventilation syndrome. Furthermore, individuals with obesity or obstructive sleep apnea have a blunted hypercapnic ventilatory response (HCVR) (120), which is indicative of impaired chemosensitivity, as HCVR is a measure of how well an individual responds …

Integrating Remote Sensing And Machine Learning To Determine Past, Current And Future Crop Water Use From The Nubian Sandstone Aquifer System, Moaz Ishag

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The agriculture sector is a significant consumer of water, and sustainable water use begins with monitoring irrigated land. Delineating irrigated land supports decision-makers and promotes the sustainable use of this crucial resource. This study focuses on the Nubian Sandstone Aquifer System (NSAS), the largest aquifers in the world, which spans Egypt, Sudan, Libya, and Chad. The study aims to: 1) quantify the increase in irrigated hectares (both pivot and non-pivot) from 2000-2001 to 2023-2024; 2) identify major irrigated crop types and their water requirements; and 3) quantify groundwater crop water use from the NSAS using remote sensing via the Google …

Uncovering Over 150 Years Of Herbarium Specimen Data To Examine The Impacts Of Climate Change On Alpine Plant Phenology In The Southern Rocky Mountains, Erin Berkowitz

Museum Studies Theses

Due to generalized botanical collecting practices, herbarium specimens provide important long term data dating back hundreds of years. The historical data preserved in herbaria can be used to study biological responses to climate change, such as changes in plant phenology, across various habitats including understudied alpine ecosystems. This study will use historical data from over one hundred years of plant collecting, along with updated collecting and community science efforts, to investigate how climate change is influencing alpine plant phenology in the Southern Rocky Mountains. These records will be analyzed alongside historical climate data to explore phenological sensitivities to climate change, …

Structural Analysis Of Dj-1 Glyoxalase Activity By Mix-And-Inject Serial Synchrotron Crystallography, Coleman Dolamore

Department of Biochemistry: Dissertations, Theses, and Student Research

Observing enzyme structures while they are catalyzing a reaction has been a major goal of both enzymology and structural biology for decades. With the advent of time-resolved serial X-ray crystallography using X-ray free electron lasers (XFELs) and synchrotron sources, enzyme reactions can be monitored in real time in crystallo. Here, we use two different approaches to study related enzymes involved in methylglyoxal detoxification. For human DJ-1, we used pink beam mix-and-inject serial crystallography (MISC) at the Advanced Photon Source (BioCARS 14-ID) to probe the controversial mechanism of DJ-1’s action on methylglyoxal by using serial Laue diffraction. The high flux …

A Deep Learning Method To Predict Bacterial Adp-Ribosyltransferase Toxins, Dandan Zheng, Siyu Zhou, Lihong Chen, Guansong Pang, Jian Yang

Research Collection School Of Computing and Information Systems

Motivation: ADP-ribosylation is a critical modification involved in regulating diverse cellular processes, including chromatin structure regulation, RNA transcription, and cell death. Bacterial ADP-ribosyltransferase toxins (bARTTs) serve as potent virulence factors that orchestrate the manipulation of host cell functions to facilitate bacterial pathogenesis. Despite their pivotal role, the bioinformatic identification of novel bARTTs poses a formidable challenge due to limited verified data and the inherent sequence diversity among bARTT members. Results: We proposed a deep learning-based model, ARTNet, specifically engineered to predict bARTTs from bacterial genomes. Initially, we introduced an effective data augmentation method to address the issue of data scarcity …

The Ecological Applications Of Δ17O: Validating A Novel Technique For Assessing Animal Metabolism And Water Intake, Zachary Taylor Steele

Biological Sciences Theses & Dissertations

Animals obtain water via different processes such as drinking, consuming food, and through metabolic processes like at the end of the electron transport chain when atmospheric oxygen is combined with hydrogen to form ‘cytochrome oxidase water’. Assessing animal water flux and metabolism has mainly been conducted via stable isotope analysis (SIA) of biological samples using hydrogen (²H) and oxygen isotopes (¹⁶O, ¹⁷O, and ¹⁸O; Δ¹⁷O), typically using enriched isotope tracers. However, natural abundance isotope modeling offers an approach for conducting SIA of biological samples that differs because it relies on the unique, …

Use Of Ultrasound For Identification Of Musculoskeletal Injury In Active Populations, Kathleen Karen Hogan

Rehabilitation Sciences Theses & Dissertations

Lower extremity tendinopathies and fasciopathy conditions are prevalent amongst sedentary individuals,^1,2 athletes,^3,4 elite runners,^5,6and military personnel⁷ and can lead to chronic disability and reduction in quality of life. ^8,9 Both tendinopathy and fasciopathy are thought to be mechanical in nature and caused by acute or chronic overloading leading to a disruption in the uniformity of the soft tissue structure. Ultrasound imaging has gained popularity in the last decade among many sports medicine clinicians to gain information regarding tissue structure for musculoskeletal injury (MKSI) management. Ultrasound imaging allows for the evaluation of in-vivo structures and at …

Effects Of Ordered Eating On Various Postprandial Measures, Brian K. Ferguson

Human Movement Studies & Special Education Theses & Dissertations

Postprandial glucose (PPG) is an indicator of acute and chronic overall health, and aberrations in glucose, insulin, and other postprandial (PP) markers are common in obesity and cardiometabolic disorders. Chronically elevated glucose can lead to a number of health problems such as type 2 diabetes mellitus. Additionally, PPG responses can impact substrate utilization responses to exercise, which may have implications for both healthy and diseased populations. One potentially simple lifestyle modification to alter PPG and related markers is to change the order in which foods are consumed within meals. The purpose of this dissertation was to further explore the impact …

Study Of Atypical Alarmone Synthesis In Clostridioides Difficile, Declan Nathaniel Butler

Chemistry & Biochemistry Theses & Dissertations

Clostridioides difficile is a Gram-positive anaerobic bacterium that causes infections in humans that costs healthcare systems billions per year. C. difficile infection has high rates of recurrence due to multiple antibiotic resistance. When bacteria are in stressful environments, they produce hyperphosphorylated guanosine ribonucleotide signaling molecules called alarmones. The accumulation of alarmones activates the stringent response (SR), in which bacterial cells induce transcription of stress survival genes to delay growth and replication. The C. difficile SR is regulated by enzymatic activity of a bifunctional synthetase/hydrolase, RelA/SpoT homolog (RSH), and a monofunctional small alarmone synthetase (RelQ). Additionally, the SR is potentially regulated …

Raemap: Real-Time Advanced Eye Movements Analysis Pipeline, Gavindya Jayawardena

Computer Science Theses & Dissertations

Eye-tracking offers insights into human cognition and attention allocation by capturing and interpreting eye movements. Traditionally, eye tracking research has relied on offline analysis methods, requiring post-experiment processing to derive insights from gaze data. This approach limits its applicability in contexts requiring immediate feedback and adaptive responses based on real-time gaze behaviors. Recent advancements in computing power and eye tracking technology have enabled a shift towards real-time analysis, allowing modern systems to interpret gaze data with minimal latency, facilitating integration into interactive applications and experiments. Despite these advancements, existing tools often face challenges with real-time computation of advanced gaze measures …

Mechanisms Of Fatigability In Individuals With Prediabetes And The Effect Of Dietary Nitrate Supplementation, Blaine Evan Arney

Dissertations (1934 -)

Prediabetes is characterized as elevated blood glucose levels below the clinical threshold for type 2 diabetes. Previous research identified an increased fatigability (exercise-induced reduction in limb force or power) of the lower limb muscles in individuals with prediabetes which can impair physical function and lead to reduced physical activity. The mechanisms of fatigability in people with prediabetes are not known but are likely related to suggested vascular dysfunction in prediabetes. An important healthcare goal is to determine effective treatments to offset the increased fatigability in people with prediabetes to improve daily function. The purpose of this dissertation was to determine …

Brome Grass And Its Management In Crops, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Brome grass (Bromus spp.) is one of the most competitive grass weeds in wheat. Over the last 10 years, brome grass has increased in importance to a greater extent than any other weed species in Australia.

Recent changes in crop production systems, increased conservation tillage, a lack of available selective herbicides, and a decline in system diversity have seen an increase in the importance of this weed in Western Australia (WA).

Seed is the main source of brome grass infestation and, as few seeds remain dormant, the most important aspect of management is to reduce its seed bank.

Annual Ryegrass And Its Management In Crops, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Annual ryegrass (Lolium rigidum) is one of the most serious and costly weeds of annual winter cropping systems in southern Australia. It is highly competitive and can compete with a crop as early as the 2-leaf stage.

Annual ryegrass is a winter to spring growing weed that can emerge from late autumn to early spring. The number of emergence flushes and the density of plants that emerge are related to initial seedbank levels and the frequency and amount of rainfall.

Ceramide-Mediated Orchestration Of Oxidative Stress Response Through Filopodia-Derived Small Extracellular Vesicles, Zainuddin Quadri, Ahmed Elsherbini, Simone M. Crivelli, Salim S. El-Amouri, Priyanka Tripathi, Zhihui Zhu, Xiaojia Ren, Liping Zhang, Stefka D. Spassieva, Mariana N. Nikolova‑Karakashian, Erhard Bieberich

Markey Cancer Center Faculty Publications

Extracellular vesicles (EVs) are shed from the plasma membrane, but the regulation and function of these EVs remain unclear. We found that oxidative stress induced by H 2 O 2 in Hela cells stimulated filopodia formation and the secretion of EVs. EVs were small (150 nm) and labeled for CD44, indicating that they were derived from filopodia. Filopodia-derived small EVs (sEVs) were enriched with the sphingolipid ceramide, consistent with increased ceramide in the plasma membrane of filopo- dia. Ceramide was colocalized with neutral sphingomyelinase 2 (nSMase2) and acid sphingomyelinase (ASM), two sphingomyelinases generating ceramide at the plasma membrane. Inhibition of …

Soxb1 Transcription Factors Are Essential For Initiating And Maintaining Neural Plate Border Gene Expression, Elizabeth Schock, Joshua R. York, Austin P. Li, Ashlyn Y. Tu, Carole Labonne

Markey Cancer Center Faculty Publications

SoxB1 transcription factors (Sox2/3) are well known for their role in early neural fate specification in the embryo, but little is known about functional roles for SoxB1 factors in non-neural ectodermal cell types, such as the neural plate border (NPB). Using Xenopus laevis, we set out to determine whether SoxB1 transcription factors have a regulatory function in NPB formation. Here, we show that SoxB1 factors are necessary for NPB formation, and that prolonged SoxB1 factor activity blocks the transition from a NPB to a neural crest state. Using ChIP-seq, we demonstrate that Sox3 is enriched upstream of NPB genes in …

Diffuse Reflectance Spectroscopy For Optical Characterizations Of Orthotopic Head And Neck Cancer Models In Vivo, Pranto Soumik Saha, Jing Yan, Caigang Zhu

Markey Cancer Center Faculty Publications

We demonstrated an easy-to-build, portable diffuse reflectance spectroscopy device along with a Monte Carlo inverse model to quantify tissue absorption and scattering-based parameters of orthotopic head and neck cancer models in vivo. Both tissue-mimicking phantom studies and animal studies were conducted to verify the optical spectroscopy system and Monte Carlo inverse model for the accurate extraction of tissue optical properties. For the first time, we reported the tissue absorption and scattering coefficients of mouse normal tongue tissues and tongue tumor tissues. Our in vivo animal studies showed reduced total hemoglobin concentration, lower tissue vascular oxygen saturation, and increased tissue scattering …

A Probabilistic Approach To Estimate The Temporal Order Of Pathway Mutations Accounting For Intra-Tumor Heterogeneity, Menghan Wang, Yanqi Xie, Jinpeng Liu, Austin Li, Li Chen, Arnold Stromberg, Susanne Arnold, Chunming Liu, Chi Wang

Markey Cancer Center Faculty Publications

The development of cancer involves the accumulation of somatic mutations in several essential biological pathways. Delineating the temporal order of pathway mutations during tumori- genesis is crucial for comprehending the biological mechanisms underlying cancer development and identifying potential targets for therapeutic intervention. Several computational and statistical methods have been introduced for estimating the order of somatic mutations based on mutation profile data from a cohort of patients. However, one major issue of current methods is that they do not take into account intra-tumor heterogeneity (ITH), which limits their ability to accurately discern the order of pathway mutations. To address this …

Response To Replication Stress And Maintenance Of Genome Stability By Wrn, The Werner Syndrome Protein, David K. Orren, Amrita Machwe

Markey Cancer Center Faculty Publications

Werner syndrome (WS) is an autosomal recessive disease caused by loss of function of WRN. WS is a segmental progeroid disease and shows early onset or increased frequency of many characteristics of normal aging. WRN possesses helicase, annealing, strand exchange, and exonuclease activities and acts on a variety of DNA substrates, even complex replication and re- combination intermediates. Here, we review the genetics, biochemistry, and probably physiological functions of the WRN protein. Although its precise role is unclear, evidence suggests WRN plays a role in pathways that respond to replication stress and maintain genome stability particularly in telomeric regions.

Assessing Soil Properties For Optimized Irrigation Development In Sudan, Northern Africa, Suhib Hamid, Derek M. Heeren, Aaron R. Mittelstet, Saleh Taghvaeian, Randall S. Ritzema

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Sustainable irrigation methods maximize agricultural productivity in Northern African countries like Sudan. This project evaluates soil properties to develop optimized irrigation strategies for Sudan's arid and semi-arid climate. Assessing soil characteristics such as texture, infiltration rates, and nutrient content informs the selection of modern irrigation systems. Data from 3,192 locations, collected using GPS and ring infiltrometers for infiltration rates, alongside laboratory analyses for soil properties, were employed. A ranking system determined suitable irrigation systems for specific soil classes, considering factors like slope, wind, crop tolerance, available water capacity (AWC), drainage, germination, capital cost, labor cost, maintenance, and evapotranspiration (ET). The …

Investigating The Antimicrobial Potential Of Bovine Lactoferrin Against The Neonatal Pathogen, Staphylococcus Capitis, Anna Desmond, Lesley Cotter, Des Field, Fiona O'Halloran

Department of Biological Sciences Publications

Lactoferrin is an antimicrobial glycoprotein that demonstrates a broad-spectrum of activity against a wide variety of clinical pathogens. This study investigated the potential of bovine lactoferrin (bLf) against multidrug resistant Staphylococcus capitis (S. capitis) strains. Growth curve analysis and time-kill curves demonstrated that at 750 µg ml−1 lactoferrin significantly inhibited (50.6%, P < 0.05) the growth of most isolates tested (90%), and this effect was based on a bacteriostatic mechanism. At the same concentration, bLf also significantly inhibited (30%, P < 0.05) biofilm formation in 40% of strains tested. Combinations of bLf with selected antibiotics were assessed for enhanced antimicrobial activity using growth curves. BLf combined with β-lactam antibiotics reduced the growth of S. capitis strains, however, the effects were not significant. BLf displays antimicrobial effects against multidrug resistant S. capitis isolates, but with strain-specific effects.

Decisions, Decisions: Dna Damage Induced Modification Of P53 And The Effect Of Modifications On P53 Differential Binding To The Dna, Savannah Gabrielle Keating

Theses and Dissertations

p53 is the most frequently mutated tumor suppressor protein in cancer. It is highly regulated, most often via post-translational modifications (PTMs). Different pathways and target genes are differentially activated by p53 and there is much left to understand how this occurs. The regulation of p53 at the post-translational level is incredibly important to its DNA damage response, and, therefore, it is understood that PTMs are often involved in the differential activation of pathways by p53. This project aimed to identify some of the types and locations of post-translational modifications on p53 in response to different modes of DNA damage with …

Association Of Candidate Single-Nucleotide Polymorphism Genotypes With Plasma And Skin Carotenoid Concentrations In Adults Provided A Lycopene-Rich Juice, Anna C Norman, Daniel G Palmer, Nancy E Moran, James N Roemmich, Shanon L Casperson

Children’s Nutrition Research Center Staff Publications

Background: Carotenoids are fat-soluble phytochemicals with biological roles, including ultraviolet protective functions in skin. Spectroscopic skin carotenoid measurements can also serve as a noninvasive biomarker for carotenoid consumption. Single-nucleotide polymorphisms (SNPs) in metabolic genes are associated with human plasma carotenoid concentrations; however, their relationships with skin carotenoid concentrations are unknown.

Objectives: The objective of this study was to determine the relationship between 13 candidate SNPs with skin and plasma carotenoid concentrations before and after a carotenoid-rich tomato juice intervention.

Methods: In this randomized, controlled trial, participants (n = 80) were provided with lycopene-rich vegetable juice providing low (13.1 mg), medium …

Enhancing Sustainable Development Goals Through Future Vapor Pressure Deficit Analysis In The Nile River Basin, Surendra Maharjan, Wenzhao Li, Shahryar Fazli, Hani Sewilam, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Vapor Pressure Deficit (VPD) is crucial in meteorology and agriculture for understanding plant-environment interactions. Its application as an indicator in agricultural practices notably advances Sustainable Development Goals such as Zero Hunger (SDG 2) and Climate Action (SDG 13). This research focuses on the impact of climate change on agricultural productivity and food security in the Nile River Basin (NRB), emphasizing the role of VPD, temperature, and precipitation. Utilizing Coupled Model Intercomparison Project Phase 6 (CMIP6) datasets from NEX-GDDP-CMIP6, the study analyzes key climatic variables that influence agricultural conditions. The study applies the Mann-Kendall test to evaluate VPD trends from 2000 …

Investigating The Abilities Of Potentially Beneficial Bacteria For Increasing Nitrogen-Use Efficiency In Maize, Lexie R. Foster

School of Biological Sciences: Dissertations, Theses, and Student Research

Global agriculture relies heavily on the use of synthetic nitrogen fertilizer to meet the current global food demand. Unfortunately, the average nitrogen-use efficiency (NUE) of maize (Zea mays) is approximately 50 percent. Improving the NUE of maize is essential for meeting the growing global food demand while also decreasing the negative environmental impacts caused by losses of nitrogen fertilizer due to runoff and volatilization. Harnessing the symbiotic relationship between plants and the soil microbiome may be one method for increasing the NUE in crops such as maize. In the present study, a set of potentially beneficial bacterial species …

New Insights On Hybridization In Potamogeton Floridanus (The Florida Pondweed), Kaitlyn R. Sampson

Graduate Theses and Dissertations (2019 - present)

Freshwater ecosystems are some of the most important and highest threatened habitats in the world, and aquatic plants play an important, but often-overlooked, role in maintaining them. Potamogeton is a diverse and ecologically important aquatic plant genus well known for taxonomic difficulty and rampant hybridization. lbis study aimed to 1) test the hypothesis that Potamogeton jloridanus (Florida pondweed) is a hybrid between P. oakesianus and P. pulcher, and 2) to investigate correlations in ecological conditions for the focal species. This study revealed the discovery of a new population of P. jloridanus in Big Coldwater Creek in Santa Rosa Co., FL, …

Heat Stress Changes The Bovine Methylome And Transcriptome And Investigation Of Two Novel Genetic Defects In Cattle, Rachel Renae Reith

Department of Animal Science: Dissertations, Theses, and Student Research

Heat stress is a major concern for livestock producers due to its negative impact on animal health and productivity. Heat stress does so by altering expression of genes through different regulatory mechanisms such as DNA methylation. Understanding how heat stress alters gene expression will help elucidate the genetic basis of physiological changes as well as identify targets for possible heat stress mitigation. The purpose of the first study was to understand how heat stress alters the adipose and skeletal muscle transcriptomes in zilpaterol-fed Brahman, as zilpaterol improves muscle growth and may mitigate the effects of heat stress. Differential expression and …