Life Sciences Commons^™

Forest Defoliation By And Invasive Outbreak Insect: Catastrophic Consequences For A Charismatic Mega Moth, Richard L. Lindroth, Mark R. Zierden, Clay J. Morrow, Patricia C. Fernandez

Aspen Bibliography

Earth is now experiencing declines in insect abundance and diversity unparalleled in human history. The drivers underlying those declines are many, complex, and incompletely known. Here, using a natural experiment, we report the first test of the hypothesis that forest defoliation by an invasive outbreak insect compromises the fitness of a native insect via damage-induced increases in toxicity of the forest canopy. We demonstrate that defoliation by the invasive spongy moth (Lymantria dispar) elicits an average 8.4-fold increase in foliar defense expression among aspen (Populus tremuloides) genotypes. In turn, elevated defense dramatically reduces survivorship, feeding, and …

Development Of Targeted, Statin Loaded Nanoparticle Delivery Systems, Stephanie Judkins

Master's Theses

Statins lower endogenous production of cholesterol through their inhibition of the mevalonate pathway. Lipophilic statins in particular exhibit anti-inflammatory effects at supraphysiological doses by preventing downstream signaling through small molecule GTPases. Interest lies in development of statins as anti-inflammatory therapeutics, however, achieving effective doses locally incurs serious off-target effects. Our goal was to develop and optimize a targeted delivery system to traffic these drugs to inflamed sites and unload the drug at effective concentrations while avoiding systemic exposure. Lipophilic statins (lovastatin, simvastatin, and atorvastatin) were incorporated into polymeric nanoparticles (NPs) due to their hydrophobicity. We have shown that statins released …

Investigating The Roles Of Sirtuins During Cvb3 Infection And Innate Immunity, Bridget M. Hulsebosch

Master's Theses

Coxsackievirus B3 (CVB3) is an enterovirus that chronically infects heart tissue, leading to dilated cardiomyopathy. However, no vaccines or antivirals exist for CVB3 infection or to treat CVB3-induced dilated cardiomyopathy, except for a heart transplant. Sirtuins (SIRTs), class III lysine deacetylases, have diverse and complex roles in cellular functions, contributing to energy production, NF-κB signaling, cell cycle regulation, and inflammation. Previous studies showed that SIRTs are essential for hepatitis B and A viral replication and regulate the IFN response during viral infection. However, the role of SIRT1 and 2 during CVB3 infection is entirely unknown. CVB3-infected SIRT1 and SIRT2 knockout …

Investigating The Oncogenic Role Of Nkx3.1 In Prostate Cancer, Audris Budreika

Master's Theses

Prostate cancer is a unique disease in that it is fueled by androgen receptor (AR) signaling. In the clinic, AR signaling is disrupted by removing androgens via chemical castration and employing AR-antagonists such as enzalutamide. Unfortunately, these therapies are short-lived, resistance sets in, and tumors evolve to a form of the disease called castration-resistant prostate cancer (CRPC). At this stage, therapeutic options are virtually exhausted and palliative care is pursued. There is a growing need to characterize other molecular players that cooperate with AR or play a unique role in CRPC on their own. One such potential candidate is the …

Characterization Of Cd11c-Expressing Gut-Associated T Cells, Ana Cvetkovic

Master's Theses

Our immune system is a remarkable defense network that protects our bodies from a range of threats. Its’ intricate machinery comprises of messenger and effector cells that work together to coordinate an effective immune response. Traditionally, T lymphocytes have been considered effectors: they receive instructions from messengers, known as Antigen Presenting Cells (APCs), that alter T-cell activation state and behavior. In the gut, Dendritic Cells (DCs) are the APCs that most often instruct T cells. To accomplish their messenger role, DCs express CD11c at high levels, a surface marker involved in cell-to-cell signaling and phagocytosis. As such, CD11c expression has …

The Effects Of Conditional Loss Of Myosin Binding Protein H-Like On Cardiac Function, Hope Victoria Burnham

Master's Theses

Dilated cardiomyopathy (DCM) is a common cause of heart failure with a strong hereditary component. Mutations in the myosin-binding protein H-like (MYBPHL) gene are linked to hereditary DCM, atrial fibrillation, and atrioventricular arrhythmias. MyBP-HL is a sarcomeric protein that is highly expressed in the atria with only scarce, distinct clusters of MyBP-HL positive cells in the ventricles, mostly in or surrounding the ventricular conduction system (VCS). Constitutive knock-out of MyBP-HL in mice causes atrial dilation, ventricular dysfunction, arrhythmia, and DCM. Whether MyBP-HL plays a developmental role, or if knock-down in adulthood will recapitulate a similar phenotype has yet to be …

Testing Strategies To Develop A Mucosal T Cell Inducing Vaccine For Hiv-1, Reynolds Boone

Master's Theses

Despite numerous efforts, HIV vaccine is still not available. Because HIV Envelop protein varies among subtypes and isolates, it is impossible for one vaccine against HIV Env to induce neutralizing antibodies against all subtypes and isolates. Previous vaccine attempts inducing HIV-specific CD4+ T cells have even been shown to enhance the HIV infection because HIV preferentially infects activated CD4+ T cells. It is known that CD8+ cytotoxic T cells (CTLs) play an important role in controlling HIV infections. We hypothesize that a vaccine inducing only CD8+ CTLs but not CD4+ T cells will be effective in preventing HIV infections. Thus, …

The Anti-Inflammatory Properties Of Exopolysaccharide From Bacillus Subtilis, Nikolay Todorov

Master's Theses

Commensal microorganisms can be used therapeutically to ameliorate a variety of diseases. The laboratory of Dr. Katherine L. Knight studies these therapeutic properties in the commensal microorganism known as Bacillus subtilis. Previously, the Knight lab has demonstrated that B. subtilis can protect against colitis caused by Citrobacter rodentium suggesting that B. subtilis possesses anti-inflammatory properties. These properties are specific to the polysaccharide capsule of the microorganism known as exopolysaccharide or EPS. EPS can be produced and purified for study. EPS has been demonstrated to be protective in a variety of diseases such as allergic eosinophilia, sepsis, Graft versus Host disease, …

08.19.2024 Orsp Connect, Liz Williamson

ORED Newsletter

Lei Cao NSF award

FDP Meeting Summaries Available

August LINC Lunch

Collaboration Opportunities

Actuarial Implications Of Prevented Planting Coverage, Dylan Turner, Francis Tsiboe, Hunter D. Biram, Lawson Connor

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Within the Federal Crop Insurance Program (FCIP), prevented planting (PP) coverage provides payments for pre-planting costs associated with crops that ultimately cannot be planted due to adverse weather. PP indemnities, which are not considered production losses within the FCIP rating methodology, influence premium rates differently than typical losses. This study utilizes a panel data set consisting of approximately 77,697 county level observations from RMA's summary of business to identify the relationship between the prevalence of prevent plant use and a number of actuarial related outcomes. Overall, we find that increases in the share of total indemnities attributable to PP claims …

Illuminating The Twilight Zone: In-Situ Imaging Facilitates Discovery Of Mesopelagic Plankton Dynamics And Community Structure, Alexander Barth

Theses and Dissertations

Ocean ecosystems provide both food and regulation of climate. Much of ocean ecosystem functioning occurs through processes in the pelagic, where plankton are the dominant organisms. While plankton have been researched for several centuries, recent advances in technology are greatly improving the ability to better understand plankton and how their communities are structured. Specifically, in-situ imaging allows oceanographers and plankton ecologists to better sample fragile taxa and record organisms’ true traits at high frequencies. These tools can facilitate investigation into new topics in plankton ecology and better understand critically understudied habitats, like the mesopelagic of open-ocean systems. However, in-situ imaging …

Elucidating Enterococcal Mechanisms Underlying Recurrent Urinary Tract Infections, Baylie Hochstedler-Kramer

Dissertations

Urinary tract infection (UTI) is one of the most common bacterial infections globally and can lead to life-threatening complications. UTI disproportionately affects females, with the majority of females being treated for at least one UTI in their lifetime. Of the females who develop UTI in their lifetime, almost one third will develop recurrence within six months after initial treatment and many will struggle with UTI recurrence for long spans of their lives. Although recurrent UTI has previously been attributed to persistence of Escherichia coli, recent studies utilizing more sensitive detection methods have shown Enterococcus faecalis is strongly associated with recurrent …

Absolute Quantification Of Human Myosin And Titin Isoforms In Disease By A Mass Spectrometry Approach, Nitha Aima Muntu

Master's Theses

Heart failure (HF) is the leading cause of death worldwide and produces an immense economic burden. Dilated cardiomyopathy (DCM), characterized by contractile weakness and chamber dilation, is a significant cause of HF. The sarcomeric proteins play a crucial role in regulating the contractile properties of the heart. As the primary motor protein, the expression of cardiac myosin isoforms (α-MHC and β-MHC) dictates the performance and cardiac energy utilization of the sarcomere. Titin is the giant spring protein in the sarcomere, and titin isoform expression impacts the passive stiffness of the myocardium. Small changes in the α-MHC/β-MHC and titin isoform ratios …

A Comprehensive Review And Meta-Analysis Of Breast Cancer Gene Expression Data: Understanding The Molecular Complexity Of A Silent Epidemic, Ifeanyichukwu O. Nwosu

Theses and Dissertations

Breast cancer is the most frequently diagnosed cancer type and the leading cause of cancer death; it accounts for 23% of total cases and 14% of deaths, with over 2 million new cases diagnosed and 600,000 deaths in 2020. This is a huge public health burden. Traditionally, clinical subtypes of breast cancer are defined based on a patient's immunohistochemistry profile. These profiles are determined by the combined expression status of three receptors: estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. It has also been observed that women of African descent, more commonly present with ER, PR and …

Sleep Restores An Optimal Computational Regime In Cortical Networks, Yifan Xu

Arts & Sciences Graduate Student Theses and Dissertations

Computation within the brain necessitates consistency and robustness, as no complex system can function effectively without these fundamental attributes. However, maintaining this robustness requires active efforts due to the inevitable perturbations (Turrigiano & Nelson, 2004). To unlock the unifying principle of how brain works, it is essential to identify an optimal set point that the system strives to maintain and restore, ensuring stability and functionality. Our lab recently demonstrated that cortical dynamics are homeostatically organized around criticality (Ma et al., 2019), a computational regime that optimizes information processing, such as information capacity and dynamic range (Shew & Plenz, 2013). This …

Association Mapping Of Important Agronomic Traits In Mucuna Pruriens (L.) Dc., Patrush Lepcha, Mahesh Shekhar, Leelambika Murugesan, Mahammad Jaheer, Ratan Chopra, Vikas Belamkar, Narayana Sathyanarayana

Department of Agronomy and Horticulture: Faculty Publications

Background The tropical legume Mucuna pruriens (L.) DC. can meet three agricultural needs: low-cost protein, highvalue medicines, and green manure or cover crops. But like other underutilized crops, it needs more modern breeding resources. Identifying marker-trait associations (MTAs) can facilitate marker-assisted breeding and crop improvement. Recent studies have demonstrated the feasibility of identifying MTAs using a small number of accessions (< 100). We have characterized a panel of 70 M. pruriens accessions across two consecutive years and performed association analysis for 16 phenotypic traits related to seed (seed length, seed width, seed thickness, seed yield per plant, hundred seed weight); pod (pod length, pod width, number of pods …

Design, Synthesis, And Evaluation Of Oleyl-Wrh Peptides For Sirna Delivery, Mrigank Shekhar Rai, Muhammad Imran Sajid, Jonathan Moreno, Keykavous Parang, Rakesh Kumar Tiwari

Pharmacy Faculty Articles and Research

Delivering nucleic acid therapeutics across cell membranes is a significant challenge. Cell-penetrating peptides (CPPs) containing arginine (R), tryptophan (W), and histidine (H) show promise for siRNA delivery. To improve siRNA delivery and silence a model STAT3 gene, we hypothesized that oleyl acylation to CPPs, specifically (WRH)n, would enhance STAT3 silencing efficiency in breast and ovarian cancer cells. Using Fmoc/tBu solid-phase peptide chemistry, we synthesized, purified, and characterized the oleyl-conjugated (WRH)n (n = 1–4) peptides. The peptide/siRNA complexes were non-cytotoxic at N/P 40 (~20 μM) against MDA-MB-231, MCF-7, SK-OV-3, and HEK-293 cells after 72 h incubation. All peptide/siRNA complexes showed serum …

Tame Your Inner Flame: A Sample Nutrition Intervention Protocol For Firefighters, Rachel Keating-Fleming

Department of Nutrition Student Projects

Firefighters are an essential part of every community that perform life saving duties and critical work to sustain everyday life. These individuals perform strenuous work every shift and are under larger amounts of job stress compared to other professions. It has been found that firefighters have increased risk of cardiovascular disease and mortality by sudden cardiac death. Studies have found that this risk comes from factors such as shift work, increased job stress, lack of time to eat a proper diet, and lack of time to engage in physical activity. A Mediterranean diet plan shows promise as an improvement strategy …

Challenges In Assessing Repellency Via The Behavioral Response By The Global Pest Tribolium Castaneum To Protect Stored Grains, Leslie Catherine Rault, William R. Morrison Iii, Alison R. Gerken, Georgina Bingham

Department of Entomology: Faculty Publications

Background: Food security is an increasingly pressing global issue, and by 2050, food production will not be sufficient to feed the growing population. Part of global food insecurity can be attributed to post-harvest losses, including quantity and quality losses caused by stored-product pests like insects. It is thus timely to find management strategies to mitigate these losses and counteract food insecurity. The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), a global stored-product pest with a wide range of food sources, was used in this study to assess repellency to a selection of essential oils. Methods: Multiple behaviorally relevant methods …

How Advection Affects The Surface Energy Balance And Its Closure At An Irrigated Alfalfa Field, Tianxin Wang, Joseph Alfieri, Kanishka Mallick, Ariane Arias-Ortiz, Martha Anderson, Joshua B. Fisher, Manuela Girotto, Daphne Szutu, Joseph Verfaillie, Dennis Baldocchi

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Orbiting around the non-closure problem in eddy covariance, a new generation of high-resolution thermal imagery has revealed that advection may be more common than previously expected. To investigate this, we conducted an extensive study over an irrigated alfalfa field that experienced heat and moisture advection. Over the course of five analysis periods (37 days total), multiple tower arrays and profile measurements were deployed to measure the horizontal advection and vertical heat flux divergence. Latent heat flux (λE) measured at the anchor tower showed an enhancement (i.e., increase) due to both local and non-local processes. Locally, as a result of the …

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxxiii. Three New Species Of Gint From Ethiopia And Somaliland (Buthidae), František Kovařík, Hassan Sh A. Elmi, František Šťáhlavský

Euscorpius

Three new species, Gint sahil sp. n. from Somaliland and Gint abshiri sp. n. and G. derbiae sp. n. from Ethiopia are described and compared with other species of the genus. Additional information is provided on the taxonomy and distribution of the genus Gint, fully complemented with color photos of specimens of the new species, as well as of their habitats. Furthermore, alongside the analyses of external morphology and hemispermatophores, we have provided description of the karyotype of G. abshiri sp. n. This species exhibits karyotype with 2n=27. Included is distribution map and a key for the genus Gint …

Archetypal Energies And Global Mental Health, Carroy U. Ferguson

Psychology Faculty Publication Series

As a keynote speaker at the Global Mental Health Conference 2024, held at Sophia University, Costa Mesa, CA, in-person and virtually, August 16-18, 2024, my topic was "Archetypal Energies As A Framework for Self-Empowerment and Well Being". The theme of this 2024 global conference was: Enlightened Minds, Compassionate Hearts, and Embodied Wisdom. To supplement my keynote address, I wrote this blog article titled "Archetypal Energies and Global Mental Health".

Documenting Food Insecure U.S. Children In The Wake Of The Pandemic, Courtney Bir, John Lai, Nicole Olynk Widmar

Department of Agricultural Economics Faculty Publications

Many households experienced significant negative economic impacts from job or work opportunity loss during the COVID-19 pandemic, including loss of income, loss of employer-provided health insurance, possible housing insecurity, and increases in food insecurity. Results from a sample of 929 U.S. households show that 53% of households with children reported some indications of food insecurity in 2020. The food security score divergences between households with and without children are stark, with food insecurity being worse among households with children. Food insecurity for households with children was exacerbated by pandemic-era disruptions; the societal costs of these food insecurity challenges are only …

Cutaneous Squamous Cell Carcinoma Of The Head And Neck: Pathological Features And What They Mean For Prognosis And Treatment, Uma Ramesh, Elizabeth Chiang, Haleigh Stafford, Jane Buell, Frank Materia, Moran Amit, Dan Yaniv

Faculty, Staff and Student Publications

Simple Summary

Cutaneous squamous cell carcinoma (cSCC) of the head and neck, although treatable, can be highly aggressive. Certain pathological features of cSCC can drastically impact prognosis. Therefore, the presence of a few such features is considered in both staging and treating cSCC. In this review, we summarize the current literature regarding pathological prognostic indicators of cSCC, including depth of invasion, surgical margins, perineural invasion, lymphovascular invasion, extranodal extension, tumor grade, tumor subtype, premalignant lesions, and molecular markers. Furthermore, we characterize the impact of each indicator on clinical outcomes of head and neck cSCC, their role in dictating adjuvant therapy …

Time-Transgressive Microbial Diversity In A Tropical Bat Guano Accumulation, Deer Cave, Mulu, Borneo, Joyce Lundberg, Donald A. Mcfarlane

International Journal of Speleology

Bat guano accumulations in caves can constitute large deposits of organic material, particularly chitin and its degradation products, that host a diverse microbiome. Previous microbiological studies have not addressed the stratified nature of these deposits in the context of the timeframe of the decomposition process. We present such data (temperature, pH, DNA sampling of eight carbon-dated strata) for a 75 cm-deep tropical guano accumulation that undergoes complete decomposition over a timescale of ~120 years. The aerobic, surface layer of freshly-fallen guano has the greatest diversity of taxa, with Bacteroidota co-dominant with Pseudomonadota, and includes many taxa unique to this …

Sex- And Age-Specific Differences In Mice Fed A Ketogenic Diet, Kenyon W. Sprankle, Mya Knappenberger, Erica J. Locke, Jack Thompson, Madison Vinovrski, Kaylin Knapsack, Stephen C. Kolwicz Jr.

Health Sciences Faculty Publications

The ketogenic diet (KD) is a high-fat, low-carbohydrate diet that results in the elevation of serum ketone bodies, known as ketosis. This metabolic consequence has been suggested as a method for treating neurological conditions, improving exercise performance, and facilitating weight loss for overweight individuals. However, since most research primarily uses male populations, little is known about the potential sex differences during the consumption of the KD. In addition, the effects of the KD on aging are relatively unexplored. Therefore, the purpose of this study was to explore sex- and age-specific differences in mice fed the KD. Male and female C57BL/6N …

Perspectives From Caregivers For Children With Retinoblastoma: Psychosocial Concerns And Genetic Counseling, Kayla Marie Lashinger

Theses and Dissertations

Caregivers play a vital role in the care of children affected with retinoblastoma as most cases are diagnosed before the age of five years old. While previous studies have explored the psychosocial needs of caregivers of children with pediatric cancer, these have not specifically focused on retinoblastoma in the United States (US). Prior research identified the profound emotional burden in terms of depression, anxiety, guilt, isolation, and loneliness experienced by caregivers. Given previous findings and the National Cancer Institute’s recommendation for genetic counseling and testing for all individuals affected with retinoblastoma, this study aimed to assess psychosocial concerns in relation …

Preimplantation Genetic Testing For Sickle Cell Disease; Exploring The Effect Health Disparities Have On Parental Awareness And Interest, Justine Frances Manigault

Theses and Dissertations

Sickle cell disease (SCD) is a group of disorders of the hemoglobin resulting in episodes of chronic pain, pulmonary hypertension, progressive multiorgan damage, risk for stroke, and increased mortality. It is caused by biallelic pathogenic variants in the HBB gene and 1 in 12 African Americans, 1 in 100 Hispanic individuals, and 1 in 30-50 Mediterranean people are reported to be carriers of the disease and have sickle cell trait (SCT). SCD is typically diagnosed through newborn screening; however, when prospective parents are aware of their carrier status, they can conceive via in vitro fertilization (IVF) and have genetic testing …

Yoga As A Didactic Tool For Musculoskeletal Anatomy For First Year Medical Students, Jennnifer Brueckner-Collins, Scott P. Stuckey

Journal of Wellness

Introduction: Medical education requires students to adjust their study habits. Active learning has been shown to enhance understanding, especially regarding anatomy. Wellness has become increasingly emphasized for medical professionals; thus, it is important for medical students to develop wellness habits to mitigate burnout. Yoga is commonly practiced with a focus on wellness and flow of movements. Yoga as an educational and wellness tool integrated into medical gross anatomy is a worthwhile pursuit. This study describes a series of anatomy-based yoga videos designed to meld wellness activities with musculoskeletal anatomy review for preclinical medical students.

Approach: Six novel yoga …

The Experience Of Homelessness And Its Effects On Gut Eubiosis, Kamerin Villagomez

University Honors Theses

Microorganisms which populate the human gastrointestinal tract (GIT) have a profound effect on overall human health. The relationship between host health and eubiosis, or balance, of the GIT microbiome is symbiotic and can have reciprocal relationships with quality of sleep, quality of diet, stress and mental health, and the experience of pain. These specific aspects of health are unique in populations experiencing homelessness due to the multi-dimensional and complex nature of their lived experience. Very few studies explore populations experiencing homelessness, with even fewer investigating the intersection between this population and the human GIT microbiome. It is important to do …