Life Sciences Commons^™

Enhancing Cardiomyocyte Resilience To Ischemia-Reperfusion Injury: The Therapeutic Potential Of An Indole-Peptide-Tempo Conjugate (Iptc), Shanshan Hou, Xin Yan, Xiang Gao, Steffen Jockusch, K. Michael Gibson, Zhiying Shan, Lanrong Bi

Michigan Tech Publications

Ischemia/reperfusion (I/R) injury leads to apoptosis and extensive cellular and mitochondrial damage, triggered by the early generation and subsequent accumulation of mitochondrial reactive oxygen species (mtROS). This condition not only contributes to the pathology of I/R injury itself but is also implicated in a variety of other diseases, especially within the cardiovascular domain. Addressing mitochondrial oxidative stress thus emerges as a critical therapeutic target. In this context, our study introduces an indole-peptide-tempo conjugate (IPTC), a compound designed with dual functionalities: antioxidative properties and the ability to modulate autophagy. Our findings reveal that IPTC effectively shields H9C2 cardiomyocytes against hypoxia/reoxygenation (H/R) …

A New Genus Of Horn-Like Tetrigids (Orthoptera: Caelifera: Tetrigidae) From South America, Daniela Santos Martins Silva, Marcelo Ribeiro Pereira

Insecta Mundi

A new pygmy unicorn genus, Brazitettix Silva, new genus, and two new species, Brazitettix roraimae Silva, new species and Brazitettix paulista Silva, new species (Orthoptera: Caelifera: Tetrigidae) are described in Brazil. This new genus is a unique tetrigid taxon in the American continent due a notched-long and elevated head projection. The morphology of this taxon resembles Rostella Hancock, 1913 and Dravidacris Bhaskar and Kasalo, 2022 that are currently comprised into Cleostratini, an artificial group that contains biogeographic distant taxa with distinct morphological head projection.

Resumen

Un nuevo género de tetrígidos, Brazitettix Silva, nuevo género, y dos nuevas especies, …

Chromatin Conformation Capture In The Clinic: 4c-Seq/Hic Distinguishes Pathogenic From Neutral Duplications At The Gpr101 Locus, Adrian F Daly, Leslie A Dunnington, David F Rodriguez-Buritica, Erica Spiegel, Francesco Brancati, Vandana M Rawal, Hadia Hijazi, Jean-Hubert Caberg, Anna Maria Nardone, Mario Bengala, Paola Fortugno, Giulia Del Sindaco, Marta Ragonese, Helen Gould, Salvatore Cannavò, Patrick Pétrossians, Andrea Lania, James R Lupski, Albert Beckers, Constantine A Stratakis, Brynn Levy, Giampaolo Trivellin, Martin Franke

Faculty, Staff and Student Publications

BACKGROUND: X-linked acrogigantism (X-LAG; MIM: 300942) is a severe form of pituitary gigantism caused by chromosome Xq26.3 duplications involving GPR101. X-LAG-associated duplications disrupt the integrity of the topologically associating domain (TAD) containing GPR101 and lead to the formation of a neo-TAD that drives pituitary GPR101 misexpression and gigantism. As X-LAG is fully penetrant and heritable, duplications involving GPR101 identified on prenatal screening studies, like amniocentesis, can pose an interpretation challenge for medical geneticists and raise important concerns for patients and families. Therefore, providing robust information on the functional genomic impact of such duplications has important research and clinical value with …

Minimally Invasive Therapies For Knee Osteoarthritis, Uchenna Osuala, Megan H Goh, Arian Mansur, John B Smirniotopoulos, Arielle Scott, Bardia Yousefi, Alan A Sag, Allison Lax, Kevin W Park, Alexander Kheradi, Marc Sapoval, Jafar Golzarian, Peiman Habibollahi, Osman Ahmed, Shamar Young, Nariman Nezami

Faculty, Staff and Student Publications

Knee osteoarthritis (KOA) is a musculoskeletal disorder characterized by articular cartilage degeneration and chronic inflammation, affecting one in five people over 40 years old. The purpose of this study was to provide an overview of traditional and novel minimally invasive treatment options and role of artificial intelligence (AI) to streamline the diagnostic process of KOA. This literature review provides insights into the mechanisms of action, efficacy, complications, technical approaches, and recommendations to intra-articular injections (corticosteroids, hyaluronic acid, and plate rich plasma), genicular artery embolization (GAE), and genicular nerve ablation (GNA). Overall, there is mixed evidence to support the efficacy of …

Spatially Fractionated Grid Radiation Potentiates Immune-Mediated Tumor Control, Rebecca A Bekker, Nina Obertopp, Gage Redler, José Penagaricano, Jimmy J Caudell, Shari Pilon-Thomas, Heiko Enderling

Faculty, Staff and Student Publications

Background: Tumor-immune interactions shape a developing tumor and its tumor immune microenvironment (TIME) resulting in either well-infiltrated, immunologically inflamed tumor beds, or immune deserts with low levels of infiltration. The pre-treatment immune make-up of the TIME is associated with treatment outcome; immunologically inflamed tumors generally exhibit better responses to radio- and immunotherapy than non-inflamed tumors. However, radiotherapy is known to induce opposing immunological consequences, resulting in both immunostimulatory and inhibitory responses. In fact, it is thought that the radiation-induced tumoricidal immune response is curtailed by subsequent applications of radiation. It is thus conceivable that spatially fractionated radiotherapy (SFRT), administered through …

Systematics Of The Rhinolophus Landeri Complex, With Evidence For 3 Additional Afrotropical Bat Species, Bruce D. Patterson, Terrence C. Demos, Laura Torrent, Amanda L. Grunwald, Cecilia Montauban, Julian C. Kerbis Peterhans, Molly M. Mcdonough, Carl W. Dick, Michael Bartonjo, M. Corrie Schoeman, Multiple Additional Authors

Biology Faculty Publications and Presentations

Roughly a third of all horseshoe bat species (Rhinolophidae: Rhinolophus) are found in Africa, where a recent continent-wide genetic survey suggested the presence of both undescribed and apparently invalid species. Here, we focus on the R. landeri species complex and the recent elevation of R. lobatus Peters, 1852, to species rank. That action created ambiguity in the taxonomy of East African members of the group—are both R. landeri Martin, 1838, and R. lobatus sympatric in East Africa or is another, unnamed species present there? Here, we refine genetic, morphological, and behavioral characterizations of R. landeri and its erstwhile synonyms with …

Metallophytes As Guardians Of Highly Harsh Environments – Comprehensive Insights Into Silene Vulgaris Tolerance To Metals, Konrad Bąk, Maksymilian Adamczyk, Jagoda Matyjek, Anna Rusaczonek, Wojciech Borucki, Alina Wiszniewska, Ewa Muszyńska

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Harnessing The Potential Of Extremophyte Sea Aster For Environmental Challenges, Maksymilian Adamczyk, Konrad Bąk, Anna Rusaczonek, Aleksandra Koźmińska, Ewa Muszyńska, Alina Wiszniewska

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Assessing Extreme Environments: Is Diplotaxis Muralis A Key Species For The Phytomanagement Of Mercury-Contaminated Soils?, Csaba Timár, Maria Anastasia Abrudan, Mihaela Gălean, Dorina Podar

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Microbial Colonization Of New Lava Tubes Formed By The Fagradalsfjall Volcano’S Eruption (Iceland), Ettore Lopo, Francesco Sauro, Ana Zelia Miller, Bogdan Petroniu Onac, Martina Cappelletti

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

The Impact Of Temperature And Humidity On The Transcriptome Of Symbiochloris Reticulata In Relation To Lobaria Pulmonaria’S Genepools, Ioana Violeta Ardelean, Christoph Scheidegger, Mihai Miclaus

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Insights Into Past Extreme Environments Through Ancient Biomolecules, Ioana Nicoleta Meleg, Magdalena Năpăruş-Aljancic, Yuichi Naito, Hervé Bocherens, Axel Barlow

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Onsite Microbial Analyses In One Of The Most Remote Quartzite Subterranean Environment On Earth: Imawarì Yeuta On Venezuelan Tepuis, Martina Cappelletti

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Shining Light On The Invisible: Fluorescent Detection Of Reactive Oxygen Species, Alex Zuo

Research Colloquium

Background

Reactive oxygen species (ROS) play an important role in cell signaling and homeostasis. However, overproduction of ROS leads to oxidative stress, as well as other related diseases, including cancer, neurodegenerative diseases, and cardiovascular disorders. Conventional ROS detection methods are limited by their low sensitivity and low specificity in real-time measurements. Fluorescent probes can offer valuable tools to detect ROS in biological systems. This study is focused on the detection of ROS formation during hypoxia and reoxygenation.

Methods

We used fluorescence detection techniques to study ROS in isolated rodent skeletal muscle models, which were exposed to alternating hyperoxia and hypoxia. …

Investigating Therapeutic Activity Of Br-Ormeloxifene (Br-Orm) Against Cervical Cancer, Mohammed Sikander, Shabnam Malik, John Apraku, Sonam Kumari, Parvez Khan, Daniel Zubieta, Hassan Mandil, Aditya Ganju, Bhavin Chauhan, Maria C. Bell, Man Mohan Singh, Sheema Khan, Murali Yallapu, Fathi T. Halaweish, Meena Jaggi, Subhash Chauhan

Research Colloquium

Aberrant activation of β-catenin signaling is strongly associated with cancer proliferation, invasion, migration, and metastasis, thus small molecules that can inhibit this pathway might have great clinical significance. Our molecular modeling studies suggest that Ormeloxifene (ORM), a triphenylethylene molecule docks with β-catenin, and its brominated analogue (Br-ORM) bind more effectively with relatively less energy (-7.6 kcal/mol) to the active site of β-catenin as compared to parent ORM. Herein, we report the synthesis and characterization of a Br-ORM by NMR and FTIR, as well as its anti-cancer potential in cervical cancer models in vitro and in vivo. Br-ORM treatment effectively …

First Biogeochemical Study Of Mira De Aire Cave: The Biggest Karstic System In Portugal, Alba Gómez-Arias, Julio Castillo, Nicasio Tomás Jiménez-Morillo, Beatriz Cubero, Sara Gutiérrez-Patricio, Pedro N. Jimenez-Morillo, José A. Gonzalez-Perez, José M. De La Rosa, Ana Z. Miller

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Ormeloxifene Suppresses Emt In Hepatocellular Carcinoma By Targeting Β-Catenin Signaling Pathway, Shabnam Malik, Mohammed Sikander, Daniel Zubieta, Anyssa Rodriguez, Murali Yallapu, Meena Jaggi, Subhash C. Chauhan

Research Colloquium

Background: Hepatocellular carcinoma (HCC) is one of the deadliest cancers, with a five-year survival rate. While Sorafenib (SRB) is the standard therapy, it is only effective in prolonging survival for some patients, emphasizing the urgent need for alternative treatment options. Resistance to apoptosis is a defining characteristic of cancer cells and a crucial factor in cancer recurrence and relapse. Although, anticancer potential of the FDA-approved compound Ormeloxifene (ORM), which has well-defined pharmacokinetic and pharmacodynamic properties, has been explored in a variety of cancers, its efficacy in HCC has remained unknown. Therefore, the purpose of this study was to investigate the …

Sampling And Dna Analysis Of Extreme Environments, Scott Tighe

FRONTIERS UNBOUND: Exploring Extreme Environments

No abstract provided.

Enhanced Bmp Signaling Via Alk2 In Osteoclasts Decreases Bone Density In Mice, Yolanda V. Gutierrez, Hiroyuki Yamaguchi, Yuji Mishina, Yoshihiro Komatsu

Research Colloquium

Bone remodeling is a complex biological process that has been extensively studied. Bone Morphogenetic Proteins (BMPs) are recognized as one of the critical growth factors that coordinate bone remodeling. Previous studies have demonstrated that BMP signaling in osteoclasts has a positive effect on osteoclast function. However, little is known about how each BMP type I receptors control osteoclastogenesis. To investigate this question, we utilized the Cre-LoxP system to specifically activate BMP signaling through ALK2 in mice. We utilized Cathepsin K (Ctsk)-Cre driver to activate BMP signaling in osteoclasts in mice. Compared with aged- and gender-matched controls, gain-of-function of BMP mutant …

Seasonal Changes In Forage Regrowth Yield And Quality Of Three Old World Bluestems, Larry M. White

IGC Proceedings (1977-2023)

Little information is available on differences in regrowth yield and quality among Old World bluestems (Bothriochloa spp.). Harvesting was staged such that 4-week regrowth of Caucasian (B. caucasica), cv. WW-Iron Master (D, ischaemum var. i.,ischaemum) and cv, Super Cauc (D. caucasica) was harvested each week from early May through mid-September to determine forage yield, ill vitro dry matter digestibility (IVDMD) and crude protein content of leaves and stems. Forage yield peaked in late June with WW-Iron Master, Caucasian, and Super Cauc producing 1280, 11S0 and 930 kg/ho/4 weeks of leaves and S00 200 and …

Herbage Production And Quality Of Rs2, A Quackgrass (Elytrigia Repens [L.]Neski.) X Bluebunch Wheatgrass (Pseudoroegneria Spicata [Pursh.] Love) Hybrid, M R. Haferkamp, D C. Adams, E E. Grings, P O. Currie

IGC Proceedings (1977-2023)

Fertiliser and mowing effects on herbage quality and yield of the RS2 hybrid were studied. Plants were fertilised with 0, 112 or 224 kg Nlha in spring 1988 and 1989, or with 112+112kg N/ha split in spring-summer, Plants were unmowed or mowed to a SO mm stubble height on selected dates in 1988 and 1989. Maximum standing crops (SC) of unmowed plants averaged 3000 kg/ha in 1988 and 5600 kg/ha in 1989; SC were increased 1000 kg/ha by fertilisation in 1989. Crude protein (CP) exceeded 120 g/kg in unmowed herbage in 1988 and decreased from 200 g/kg in May to …

Utilization Of Tall Fescue In Pure Stand, In Association With White Clover And In Complex Mixtures, S Badoux

IGC Proceedings (1977-2023)

Tall fescue (Festuca arundinacea Schreb.) was cultivated in pure stand, in association with white clover (Trifolium repens L.) and in various complex grass-legume mixtures. Forage yield and quality were always higher in associations with white clover than in tall fescue pure stands. However, under dry conditions, the balance between the two components was difficult to maintain. In more complex mixtures cocksfoot and tall fescue tended to become dominant and to produce a forage of lower quality. In mixtures of long duration, we replaced meadow fescue by tall fescue. Total yield over 3 years was improved; the digestibility of …

Use Of Plant Height For Determining The Nutritive Value, Yield And The Optimal Use Span Of Lucerne, Lother Schmidt

IGC Proceedings (1977-2023)

Determining the ·cutting date is important for high yield, good forage quality and longevity of lucerne (Medicago media Pers.) stands. Most recommendations for the optimal use date are based on the development ,stage. The results of research conducted over 9 years show that forage quality and the dry matter (DM) yield of luccrne, at the same, developmental stage, vary considerably, depending on weather conditions during the growth period. A better criterion is the plant height of lucerne. There was a highly significant correlation between plant height and the content of crude fibre, crude protein and DM. We recommend the …

Lamb And Steer Production From Continuously Grazed Alfalfa, Jerry D. Volesky, Daniel P. Mowrey

IGC Proceedings (1977-2023)

Little is known about livestock production from alfalfa (Medicago saliva L,) under continuous grazing. Objectives were to determine lamb and steer performance, production and leaf-stem selection on continuously grazed alfalfa. Lambs and steers separately grazed 3 replicate pastures from late April through mid-August, 1991. Initial stocking densities were relatively heavy to utilise rapid spring growth after which livestock numbers were reduced to maintain alfalfa with a 20-cm stubble height. Season-long live-weight gains for the lambs and steers were 0.15 and 0.94 kg/day. Total production for lambs and steers was 557 and 529 kg/ha, Lamb diets generally contained a higher …

Impact Of Intermittent Clipping On Productivity, Forage Quality And Legume Persistence Of Reclaimed Mine Soils, M Collins, H B. Rice, R Turley, W O. Thom

IGC Proceedings (1977-2023)

The materials used as plant growth media during surface mine· reclamation are frequently low in available N, P and K and are drought­prone. Legumes are included in reclamation seeding mixtures . because of their symbiotic N2-fixation ability but competition may prevent establishment or reduce persistence. A field study was conducted to test the hypothesis that intermittent clipping of these sites would reduce grass competition and thereby maintain legume stands. In 1990 and· 1991, 4 clipping treatments were imposed, providing intervals of 2, 4; and 6 weeks between harvests. Clipping at the 2- or 4-week intervals gave equal yields that were …

Sward Height In Grazing Management: Vertical Profiles In Forage Quality, J E. Johnston, A Singh, E A. Clark

IGC Proceedings (1977-2023)

Vertical and temporal trends in herbage yield and quality were assessed in a study sown in 1989 near Elora, Ontario. In a replicated field trial with 0.1 ha paddocks, Hereford heifers rotationally grazed 2 swards to each of 2 residual grazing heights, namely, SO and 100 mm, with a requisite 200-2S0 mm entry height. Profiles in yield and in in vitro organic mailer digestibility (IVOMD) were developed from 6, 1500 cm2 quadrats per paddock, which were subjected to vertically stratified clipping, using sheep shears with a vacuum attachment. In 1990, obliging entry. at 200-2S0 mm stabilised herbage organic matter on …

Sward Height In Grazing Management: Species Adaptation, A Singh, J E. Johnston, E A. Clark

IGC Proceedings (1977-2023)

Under continental, northern conditions, yield potential of species such as perennial ryegrass (Loli11111 pere,me L.) and white clover (Trifolium repe,is L.) is considered to be inferior to that of taller, coarser species such as orchardgrass, bromegrass, timothy and alfalfa. However, recent species evaluation bas stressed management regimes characteristic of conserved feed rather than pasture production, In a replicated trial, species performance and persistence in mixture were evaluated in response to sward height-based grazing management. Starling in 1990, 2 swards constructed predominantly of fine- or coarse-stemmed grasses were grazed rotationally by cattle, using a common 20-25 cm entry height and a …

Manipulation Of Perennial Ryegrass With The Plant Growth Regulator Mefluidide, M H. Foreman, R J. Field

IGC Proceedings (1977-2023)

In an attempt to increase the nutritive quality of herbage, the plant growth regulator mefluidide was applied at low rates (0.15-0.30 kg ai/ha) to pure stands of perennial ryegrass (Lolium perenne L.) in early spring. There was a reduction in total dry matter production of 35.8% at 0.3 kg ai/ha, but because reproductive development was significantly reduced there was a useful increase in the green leaf harvest component from 1.3% to 41.6% of total dry matter. High yields of green leaf were maintained for 100 days, in contrast to untreated swards which were composed largely of stem plus inflorescence …

Interaction Among Harvest Frequency, Fertilizer And Herbicide Use With Intensively Managed Alfalfa In The North-Central Usa, O B. Hesterman, J J. Kells, P L. Tiffin

IGC Proceedings (1977-2023)

This research evaluated effects of 2 alfalfa (Medicago sativa L,) cultivars (winter hardy and moderately winter hardy), 3 levels of fertiliser (none, medium, high), 3 levels of herbicide (none, medium, high), and 2 harvest systems ( 4 harvests and 5 harvests per year) on forage yield and quality, Five years (1987-1991) of an experiment in Michigan, USA, have been completed. During the first production year (1988). forage dry matter (DM) production was similar for the 5-harvest (15.2 Mg/ha) and the 4-harvest system (14.8 Mg/ha), During subsequent years, DM production was greater in the 4-harvest than in the 5-harvest system …

Seasonal Changes Of Daily Increase In Extended Sward Height, Dry Matter, Bulk Densities And Digestible Dry Matter Of Temperature Grasses, K Watanabe, K Kita, S Saiga

IGC Proceedings (1977-2023)

Under a regime of frequent cutting, seasonal changes of the daily increase in extended sward height, dry matter (OM) bulk densities and digestible dry matter (DOM) of perennial ryegrass (Lolium perenne L.), orchardgrass (Dactylis glomerata L.) and Kentucky bluegrass (Poa pratensis L.) were followed for 5 years. Seasonal changes of the daily increase in extended sward height, DM bulk densities (daily increase in OM/daily increase in extended sward height) and DOM (daily increase in DOM/daily increase in extended sward height) of the grasses had 3 peaks and 2 troughs, as the height and densities were depressed …