Life Sciences Commons^™

Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia, Katherine Riddle

All Theses

Floral color variation within a species is a common occurrence and its maintenance has been a long-standing question in evolutionary biology. Differences in flower color between populations can be shaped by pollinator-mediated selection. Pollinators can exhibit innate and learned preferences for color that may influence floral color variation. These preferences may be molded by the visual environment in which pollinators forage. Most current understanding of how pollinators shape floral color variation involves dramatic differences in dominant pollinators between populations (e.g., hawkmoth vs. bee) and most behavioral work utilized model pollinators like honeybee and bumble bee. However, less is known about …

Informing Management Of American Shad (Alosa Sapidissima) In The Carolinas: Evaluating Novel Fish Passage Approaches And Acoustic Tracking Methods, Margaret Gaither

All Theses

Acoustic telemetry is a valuable tool for fisheries scientists to study the movements and migratory behaviors of aquatic organisms. A critical assumption of this method is that tagged fish exhibit similar behaviors and survival rates as their untagged counterparts. To investigate this, we conducted a 12-week laboratory study on adult American Shad (Alosa sapidissima) using gastroesophageal insertion of acoustic transmitters. We implanted 80 fish with dummy transmitters and monitored tag retention and survival. Our analysis, employing a mixed effects Cox’s proportional hazards model, revealed no significant differences in survival between tagged and control fish, regardless of sex. Additionally, …

The Effect Of Packaging On The Acceptance Of Natural Peanut Butter, Perian M. Reese

All Theses

The aim was to determine the effect of packaging on the acceptance of natural peanut butter, especially concerning the acceptance of the undesirable oil layer. The jars of peanut butter differed by color, label size, and inclusion of qualifying statements. 119 participants across two groups completed this study. Both groups completed an eye-tracking study in a mock grocery setting. The first group rated all five jars in appearance, expected quality, and overall rating. They were given one sample cup of peanut butter and gave hedonic liking values for color, oiliness, peanut flavor, smoothness, sweetness, and overall liking. The second group …

On Humans, Hogs, And Habitat: How Landscape Features And Vegetation Influence Wild Hog (Sus Scrofa) Detection On Private Lands And Using Existing Wild Hog Outreach Websites To Create An Effective Website For South Carolina, Andrew Jamison

All Theses

Invasive species are a source of human conflict with wildlife on a global scale and exist to the detriment of native wildlife species. Invasive species often possess the ability to respond rapidly to change and they often exist alongside humans. Wild hogs (Sus scrofa) are perhaps one of the most infamous examples of an invasive species. The spread of wild hogs necessitates effective communication with the public being impacted by them, as well as a continuation of research to learn more about wild hog behavior to promote effective management. Chapter 1 focuses on how we created a website …

Investigation Of Chondrogenesis, Myogenesis, And Osteogenesis After 2,4-Dtbp, 2-Bp, 4-Bp, 3,5-Dtbc Exposure In Zebrafish, Haley Brashears

All Theses

Synthetic phenolic antioxidants (SPAs) are used in personal care products, food packaging, and water pipes, common SPAs include 2,4-di-tert-butylphenol (2,4-DTBP), 2-tert-butyl phenol (2-BP), and 4-tert-butyl phenol (4-BP). These compounds are detected in the environment and in human biological samples. 2,4-DTBP has been detected in cord plasma, placenta, and breast milk samples. This drives the concern regarding developmental toxicity. In human iPS cells 2,4-DTBP impairs differentiation into somites and osteoblasts. Other SPAs, like 2,6-di-tert-butyl-hydroxytoluene caused increased rates of malformations including yolk and pericardial edema, and spinal curvature in 5-day post fertilization (dpf) zebrafish. …

Developing Holistic And Reduced Fertilization Guidelines For Southeastern Peach (Prunus Persica (L.) Batsch) Orchards, Richardson Bien-Aime

All Theses

Peach production in the southeastern United States requires optimizing fertilization practices to balance economic sustainability and environmental health. Current guidelines may overlook rootstock-specific nutrient requirements and fail to align with orchard-specific conditions, often resulting in overfertilization, increased costs, and environmental risks. This study, conducted at Clemson University’s Musser Fruit Research Center during 2023 and 2024, aimed to refine fertilization practices through two complementary experiments. The first experiment investigated how three rootstocks, ’MP-29’, Lovell, and Guardian, influence nutrient dynamics of ‘Carored’ peach trees. Nutrient concentrations were measured in various tree organs, including leaves, pruned wood, thinned fruitlets, and harvested fruits, along …

A Drosophila Model Of Mucopolysacchridosis Iiia, Rebecca Bishop

All Theses

Mucopolysaccharidosis IIIA (MPS IIIA) is a rare lysosomal storage disorder that arises from inability to break down heparan sulfate (HS) because of mutations in the N-sulfoglucosamine sulfohydrolase (SGSH) gene. We used a deletion mutant of the Drosophila melanogaster Sgsh gene along with three point mutations analogous to mutations observed in patients (S64W, L89P, S301P) to show an increase in the average percent of area with lysosomal puncta in the fly brains of our mutants using Lysotracker. RNA sequencing of brains of mutant and control flies showed 441 (Knockout), 337 (S64W), 155 (L89P), and 96 (S301P) differentially expressed …

Lateral Dispersal Of Adult Trichoptera: Flight Distance And Traveling Pattern, Peilin Tan

All Theses

Trichoptera species (caddisfly) are usually used as bio-indictors for surface water quality due to their sensitivity to environmental changes, and if assessing water quality with light-trapped adults, knowing their travelling patterns will improve the accuracy of such assessments. To investigate how far a caddisfly will migrate from their emerging stream, three streams in South Carolina with different widths (Wildcat Creek 1.2 m, Brasstown Creek 11.2 m and Chattooga River 26.95 m) were selected for this study with light traps placed at three distances (0 m, 50 m, 100 m) from the streams. How long and when? Both species richness and …

2024 Standard-Sized Seedless Watermelon Cultivar Evaluation In Indiana, Wenjing Guan

Midwest Vegetable Trial Reports

No abstract provided.

Primary Industries Education Foundation Australia (Piefa) Storm And Flood Industry Recovery Program: Evaluation Report, Tamara Van Der Zant, Katherine Dix

Program monitoring and evaluation

Despite the growing diversity of careers in Primary Industries, young peoples’ pursuit of agricultural careers is often hampered by outdated perceptions of the sector and its opportunities. As Primary Industries adapt to the threat of natural disasters by turning to scientific and technological solutions, a diverse, highly skilled workforce will be required. In the wake of the devastating impacts of storm and floods in early 2021 across New South Wales, the Storm and Flood Industry Recovery Program (SFIRP): Creating resilience through empowering school curriculums about primary industries careers project was developed. To aid the long-term recovery and resilience of the …

The Neural Mechanisms Of Visual Art Expertise During Different Stages Of The Creative Process: An Eeg Study, Kim N. Awa

Graduate Theses and Dissertations

Generating and drafting ideas are creative processes linked to internal neural mechanisms, such as directed attentional states, reflected in alpha band synchronization patterns. Previous research has demonstrated that these mechanisms vary based on levels of expertise during creative tasks. However, few studies have examined the multifaceted nature of ideation and externalization across multiple stages of the creative process, particularly when accounting for domain-specific expertise in imagery and figural tasks. The present study investigates alpha band synchronicity (lower and upper) across visual art backgrounds, specifically among artists, intermediates, and non-artists, during three distinct stages of the creative process: thinking of ideas, …

Analyzing Milled Rice Breakage Patterns Under Diverse Environmental Settings, Devisree Chukkapalli

Graduate Theses and Dissertations

This study comprehensively evaluates the effects of environmental conditions, transportation, and storage practices on the quality of milled rice using three cultivars: CLL16 (long grain pureline), XP753 (long grain hybrid), and Titan (medium grain pureline). Three distinct objectives guided the research: (1) assessing the impact of relative humidity (RH), temperature, and exposure duration on rice quality post-milling and post-storage; (2) analyzing seasonal and locational influences on rice quality during transportation and storage; and (3) investigating the effects of subfreezing storage on key quality attributes.
Under controlled environmental conditions, post-milling exposure (PME) caused significant quality degradation at higher RH (75% and …

Integrating Sustainability And Food Safety Through Quantitative Microbial Risk Assessment And Life Cycle Assessment Of Low-Moisture Foods, Jessica Bain

Graduate Theses and Dissertations

Food safety and sustainability are critical concerns in the food industry. However, these areas are often evaluated separately, leading to an incomplete understanding of the overall impact of food production on human health. This dissertation aims to integrate food safety with sustainability to provide a comprehensive assessment of the impact of low-moisture foods on human health, using the almond processing supply chain as a case study.

In addition to conducting life cycle assessments (LCA) of almond processing, wheat flour production, and dried apple processing, this dissertation aims to create a framework to bridge the gap between sustainability and food safety. …

Use Of Hatchery-Raised Fish In Validation Of Daily Age Estimates For Juvenile Common Snook, Philip W. Stevens, Kyle L. Williams, Meredith B. Pfennig, Ryan W. Schloesser, Alexis A. Trotter, Derek P. Crane

Biology

Objective: We investigated the validity of daily age estimates for juvenile Common Snook Centropomus undecimalis by using sectioned and sanded sagittal otoliths. Methods: Common Snook have a protracted spawning season, which is problematic for validation of daily ages because a hatch date—needed to calculate age—cannot be reasonably assigned like it can for species with a short spawning period (<30 days). To help overcome this, two readers independently counted presumed daily increments in otoliths collected from hatchery-reared Common Snook of known age (100–240 days; n = 91). Result: Differences between known ages and those estimated from otoliths were small (mean absolute difference = 3.4 days) for individuals aged 100 days, but these differences increased after 100 days, mainly due to the crowding of increments along the otolith margin. Underestimation of ages was 8% at 120 days, 29% at 180 days, and 36% at 240 days. Conclusion: Ideally, analyses based on counts of daily increments in Common Snook otoliths should be limited to fish with an age of 100 days or younger.

Recreational Fishing For Western Rock Lobster: Estimates Of Participation, Effort And Catch In 2023/24, Claire B. Smallwood, Karina L. Ryan, Alissa C. Tate

Fisheries Research Reports

The Western Rock Lobster (WRL) (Panulirus cygnus) is the most commonly caught Rock Lobster (RL) species in Western Australia. Catches from the commercial and recreational sectors are required to determine and monitor Total Allowable Commercial Catch (TACC), Total Allowable Recreational Catch (TARC) and the proportion of Allowable Harvest Level (AHL) achieved. The TARC is set at 5% of the AHL and evaluated in the harvest strategy based on a 5-year average.

Data for the recreational sector are obtained using phone-recall surveys to provide annual estimates of participation, fishing effort and retained catch (by numbers) from recreational fishers who hold a …

Rice Response To Struvite And Other Phosphorus Fertilizers In A Phosphorus-Deficient Soil Under Simulated Furrow-Irrigation, Jonathan B. Brye, Diego Della Lunga, Kristofor R. Brye

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Wastewater-recovered phosphorus (P), in the form of the mineral struvite (MgNH₄PO₄⋅6H₂O), may provide a sustainable alternative to decreasing rock-phosphate reserves. Struvite can be generated via precipitation methods, potentially reducing the amount of P runoff to aquatic ecosystems. The objective of this greenhouse tub study was to evaluate the effects of chemically- and electrochemically precipitated struvite (CPST and ECST, respectively) on above- and belowground plant response in a hybrid rice (Oryza sativa) cultivar grown using furrow-irrigation compared to other common fertilizer-P sources [i.e., triple super phosphate (TSP) and diammonium phosphate (DAP)]. Rice was …

Interpreting The Spatial Distribution Of Soil Properties With A Physically-Based Distributed Hydrological Model, Zamir Libohova, Marcelo Mancini, Hans Edwin Winzeler, Quentin D. Read, Ning Sun, Dylan Beaudette, Candiss Williams, Joshua Michael Blackstock, Sergio H.G. Silva, Nilton Curi, Kabindra Adhikari, Amanda Ashworth, Joshua O. Minai, Phillip R. Owens

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Digital soil maps are commonly data-driven as the development of physically-based models for soil mapping is difficult due to the complexity of soils. However, physically-based hydrologic models have been successful in simulating water dynamics. Since water movement is a major driver of pedogenesis, the physical rules that govern water movement might help explain and predict the spatial variation of soil properties. Here, we demonstrate the novel use of a physically-based, distributed hydrologic model to inform the spatial distribution of soil properties. The Distributed Hydrology Soil Vegetation Model (DHSVM) was utilized to simulate soil moisture content (SM) and water table …

Draft Assembly And Annotation Of The Cuban Crocodile (Crocodylus Rhombifer) Genome, Robert W. Meredith, Yoamel Milián-García, John Gatesy, Michael A. Russello, George Amato

Department of Biology Faculty Scholarship and Creative Works

Objectives: The new data provide an important genomic resource for the Critically Endangered Cuban crocodile (Crocodylus rhombifer). Cuban crocodiles are restricted to the Zapata Swamp in southern Matanzas Province, Cuba, and readily hybridize with the widespread American crocodile (Crocodylus acutus) in areas of sympatry. The reported de novo assembly will contribute to studies of crocodylian evolutionary history and provide a resource for informing Cuban crocodile conservation. Data description: The final 2.2 Gb draft genome for C. rhombifer consists of 41,387 scaffolds (contigs: N50 = 104.67 Kb; scaffold: N50-518.55 Kb). Benchmarking Universal Single-Copy Orthologs (BUSCO) identified 92.3% of the 3,354 genes …

The Effects Of Mosaicism On Biological And Clinical Markers Of Alzheimer's Disease In Adults With Down Syndrome, Laura Xicota, Lam-Ha T. Dang, Alice Lee, Sharon Krinsky-Mchale, Deborah Pang, Lisa Melilli, Sid E. O’Bryant, Rachel L. Henson, Charles Laymon, Florence Lai, H. Diana Rosas, Beau Ances, Ira Lott, Christy Hom, Bradley Christian, Sigan Hartley, Shahid Zaman, Elizabeth Head, Mark Mapstone, Zhezhen Jin, Wayne Silverman, Nicole Schupf, Benjamin Handen, Joseph H. Lee, Alzheimer’S Biomarker Consortium – Down Syndrome (Abc-Ds)

Neurology Faculty Publications

Background Individuals with Down syndrome (DS) are at high risk of early-onset Alzheimer’s disease (AD); yet, some 20 percent do not develop any signs of dementia until after 65 years or in their lifetime. Mosaicism could contribute to this phenotypic variation, where some disomic cells could lead to lower levels of gene products from chromosome 21.

Methods We examined longitudinal neuropsychological and biomarker data from two large studies of DS: the Alzheimer Biomarker Consortium–Down syndrome study (ABC-DS) (n = 357); and a legacy study (n = 468). We assessed mosaicism using karyotyping or GWAS data. Participants had data on plasma …

Targeted Long-Read Sequencing To Quantify Methylation Of The C9orf72 Repeat Expansion, Evan Udine, Nicole Finch, Mariely Dejesus-Hernandez, Jazmyne L. Jackson, Matthew C. Baker, Siva Arumugam Saravanaperumal, Eric Wieben, Mark T. W. Ebbert, Jaimin Shah, Leonard Petrucelli, Rosa Rademakers, Björn Oskarsson, Marka Van Blitterswijk

Neurology Faculty Publications

Background The gene C9orf72 harbors a non-coding hexanucleotide repeat expansion known to cause amyotrophic lateral sclerosis and frontotemporal dementia. While previous studies have estimated the length of this repeat expansion in multiple tissues, technological limitations have impeded researchers from exploring additional features, such as methylation levels.

Methods We aimed to characterize C9orf72 repeat expansions using a targeted, amplification-free long-read sequencing method. Our primary goal was to determine the presence and subsequent quantification of observed methylation in the C9orf72 repeat expansion. In addition, we measured the repeat length and purity of the expansion. To do this, we sequenced DNA extracted from …

Understanding And Intervening In Children's Tick Risk Perceptions And Protective Behaviors: A Grounded Theory, Ariel Dawdy

Department of Entomology: Dissertations, Theses, and Student Research

Children, ages 8 years and younger, are at a particularly high risk of contracting a tick-borne disease. This is partially due to the misconceptions spread about tick protective behavior and a poor understanding of how antibiotic treatment can help. Because most communities and families believe that tick control is the individual’s responsibility, looking at the interaction between ticks and human children is essential to understanding the individual’s motivations to protect themselves. To help alleviate the number of individuals contracting tick-borne diseases, misconceptions must be addressed, intervention programs implemented, and a behavioral change must occur. This study used a qualitative research …

Soybean Cyst Nematode (Heterodera Glycines Ichinohe) Virulence Assay Against Resistance Sources In Nebraska, Pratibha Karki

Department of Plant Pathology: Dissertations, Theses, and Student Research

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe), one of the major yield-limiting pathogens of soybean, is currently found in 60 counties of Nebraska that produce more than 90% of the state’s soybean. Often, yield loss occurs without any above-ground symptoms, which makes it difficult to manage. The primary management option is the use of SCN-resistant varieties. Several soybean plant introductions serve as resistance sources against SCN. Unfortunately, the single source, PI 88788, is used in approximately 90% of resistant varieties and its prolonged use has increased the virulence status of SCN in the U.S. A study was conducted to assess …

Characterization Of The Final Enzyme In The Coenzyme M Biosynthesis Pathway In Methanosarcina, Connor John Hines

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Methanogenesis is known to be one of the oldest forms of energy generation on our planet. Methanogens rely on this method of energy conservation and have evolved to metabolize a variety of substrates, including the waste products of other organisms. This feature has made methanogens beneficial to both wastewater treatment and landfill-to-energy projects. Methane itself is a greenhouse gas twenty times more potent than carbon dioxide, viewed as a particularly influential contributor to global greenhouse emissions. Climate change was known to be on the horizon throughout the 20^th century but has made itself known vocally during this 21^{st …}

Spatial Ecology Of American Bison On Vermejo Park Ranch, New Mexico, Kate Ann Asmus

School of Natural Resources: Dissertations, Theses, and Student Research

American bison (Bison bison) are a keystone species that impact grassland productivity, plant diversity, and landscape heterogeneity. We used GPS telemetry and a replicate herbivore grazing experiment on Vermejo Park Ranch in northeastern New Mexico to advance understanding of bison spatial ecology and grazing dynamics. First, we evaluated the potentially disparate influences of forage productivity and site fidelity on the size and selection of home ranges of bison. Home range size was strongly negatively influenced by forage productivity. However, home range selection was influenced more strongly by site fidelity than spatial variation in forage, as bison selected home …

The Endocrine And Body Composition Responses To Accentuated Eccentric Loading Performed In Cluster Sets During A Strength–Endurance Training Block, Kurt W. Mcdowell, Alex Long, Daniel Gahreman, D. Andrew Nelson, Nicholas J. Goode, Ryan P. Lis, Sungwon Chae, Satoshi Mizuguchi, Marten Baur, Kevin F. Breuel, Marco Duca, John P. Wagle, Andy C. Fry, Michael H. Stone

Kinesiology

Featured Application: Research on the endocrine changes from AEL training is limited, especially for long-term effects. This study suggests that combining AEL with CS might offer similar or slightly better results compared to traditional training. However, the practical challenges, such as increased training monotony and strain, may outweigh the minimal benefits. The extra cost, time, and effort required for AEL, especially in large team settings, might not be justified. Additionally, individual differences in optimal loading prescriptions could complicate its use, making traditional methods more efficient. This study investigated the endocrine responses to accentuated eccentric loading (AEL) paired with cluster sets …

An Extensive Exploration Of Generalist Microbotryum Species Through Host Interactions., Emilee Walters

College of Arts & Sciences Senior Theses

The Microbotryum species complex is a group of fungi, commonly known as anther smuts. Anther smuts are plant parasites that must invade a host plant in order to fulfill their life cycle. As the Microbotryum fungus grows, it sterilizes the host plant, such that infected plants produce fungal teliospores instead of pollen on the male reproductive organs and female reproductive structures do not mature. Microbotryum usually exists as a specialist species, however, there is evidence that several species of anther smuts can exist as generalist species. Comparisons of the infection process and the resulting response of both parasite and host …

Induced Systemic Resistance Against Nematodes By Plant Growth-Promoting Microbes In Potatoes, Cole Grannan

Master's Theses

Biofertilizers consist of plant growth-promoting microorganisms that establish symbiotic relationships with plant roots and stems. These beneficial microbes play a crucial role in enhancing plant defenses against various threats including insects, nematodes, and microbial pathogens. The purpose of this study was to investigate the effectiveness of two fungal biofertilizers (an endophytic strain of Beauveria bassiana (BA) and a mixture of arbuscular mycorrhizal (AM) fungi), specifically their ability to induce systemic resistance against plant- parasitic nematodes (PPNs) in potatoes (Solanum tuberosum cv. Masquerade) under controlled greenhouse conditions. The study utilized soil collected from Colorado State University’s San Luis Valley (SLV) Research …

Soil Data From Spring Run Conservation Area, Dayton, Ohio, Madelaine Gregory, Valerie N. Thurston, Michaela J. Woods, Ryan W. Mcewan

Data Files: Ecosystem Restoration Following Industrial Agriculture

This spreadsheet contains soil data from an experiment in the Spring Run Conservation Area in Dayton, Ohio, USA (39° 46 '42.2"N, 84° 20' 52.5"W). Details of the data collection can be found in the master's thesis "The State of Soil Streamlines Secondary Succession: Remediating Degraded Soils in a Post-Agricultural Field."

Development Of An In Vitro Squamous Metaplasia Model Using Airway Basal Stem Cells, Arash Tahmasbi

Electronic Theses, Projects, and Dissertations

Squamous metaplasia (SM) of the airway epithelium is an abnormal condition that is caused by exposure to airborne toxicants, cigarette smoke, pollutants, and pathogens. Since SM of the airway was first documented in the 1970s, data on this epithelial disease remain limited. More research is needed to fully understand this epithelial disorder and to prevent its progression into severe illnesses such as chronic bronchitis (CB), COPD, and squamous cell carcinoma. We developed 3D models of SM using airway basal stem cells (ABSCs) isolated from lung obtained from postmortem human donors. The ABSCs were cultured at the air-liquid interface (ALI) for …

The Effects Of Wildfires On Predation Risk, Perch Selection, And Background-Matching In Western Fence Lizards, Elmer Ernesto Gutierrez

Electronic Theses, Projects, and Dissertations

Fires are a naturally occurring phenomenon, driving a range of behavioral, reproductive, and morphological adaptations in wildlife. However, we are now living in a new geological epoch—the Pyrocene—characterized by human-induced extreme fire behavior. This shift results in a need to understand how animals survive and adapt in post-fire environments. In this study, we investigated the following questions: (1) Does predation risk increase in burned habitats after a fire? (2) How does dorsal darkness morphology vary between burned and unburned habitats? and (3) Do animals select perches that enhance their background-matching in post-fire environments? We used the Western Fence Lizard ( …