Life Sciences Commons^™

Honeybee Biodiversity Based On Five Mitochondrial Genes And New Evidence For Differentsublineages In Türkiye, Meral Kekeçoğlu, Songül Bi̇r, Merve Kambur Acar, Rustem Ilyasov

Turkish Journal of Veterinary & Animal Sciences

In this study, the genetic structure of honey bee populations in Türkiye was analyzed using eleven restriction endonuclease enzymes targeting the COI-COII, COI, 16S rDNA, ND5, and CYT B genes. A total of 553 worker honey bee samples were collected from 43 different districts across Türkiye, as well as from Greece, Bulgaria, and Georgia, neighboring countries of Türkiye. Analysis of the COI region using the HinfI restriction enzyme revealed the presence of two mitotypes. Type 1 was the predominant type in Türkiye, while Type 2 was detected exclusively in Hatay, a province in the southern part of the country. Notably, …

Evaluation Of The Impact Of Heat Stress At Flowering On Spikelet Fertility And Grain Quality In Barley, Camilla Beate Hill, Tefera Angessa, Sharon Westcott, Lee-Anne Mcfawn, Hamid Shirdelmoghanloo, Yong Han, Chengdao Li

Grain and Other Field Crops Research Articles

Against the backdrop of a warming climate, heat stress has become one of the most limiting factors to crop productivity and food security worldwide. The flowering stage is susceptible to high temperatures in cereal crops, leading to severe grain yield losses by decreasing fertility and seed-setting rate. Our objective was to (1) assess a global panel of 572 barley varieties with contrasting genetic backgrounds for various agronomic traits related to spikelet fertility and grain quality, (2) identify barley germplasm with superior spikelet fertility and seed set under extreme heat conditions, and (3) understand the relationship between spikelet fertility and grain …

Host Mediated Rhizobiome Engineering To Develop Locally Adapted Soil Mycorrhizal Inocula To Facilitate The Establishment Of Native Plants On Contaminated Soils, Anna Nugent

Graduate Theses & Non-Theses

The microbial community in the rhizosphere of plants plays an important role in plant establishment and survival, soil stability during stormwater runoff, and potential biofiltration of stormwater. Locally adapted fungal symbionts may further improve these outcomes for native plants at restoration sites. This study assesses the potential of host plant cultured, locally adapted soil mycorrhizal inocula to facilitate the establishment and growth of Mountain Big Sagebrush (Artemisia tridentata subsp. vaseyana) on heavy-metal contaminated soils. Soil was obtained from the rhizosphere of native plants at three sites in the West Side Soils Operable Unit of the Butte, Montana Superfund Complex. One …

Na2B12Si6Se18: A Novel B12-Cluster-Containing Quaternary Selenoborate Framework Material, Gopabandhu Panigrahi, Hunter B. Tisdale, Gregory Morrison, Hans Conrad Zur Loye

Faculty Publications

We report the successful synthesis of the new quaternary, three-dimensional Na₂B₁₂Si₆Se₁₈ material using a solid-state synthesis route. This compound is characterized by a unique framework structure that features the rare icosahedral [B₁₂]¹⁰⁺ cation which is connected via Si2Se6 polyhedral units into a 3D framework structure with Na+ ions located inside the channels. The Na₂B₁₂Si₆Se₁₈ compound crystallizes in the cubic crystal system in the space group Im, with lattice parameter a = 11.91110(10) Å and unit cell volume of 1689.88(4) ų. …

Mountain Big Sagebrush Restoration In Former Dryland Pasture, Elizabeth Bailey, Eric Thacker, Thomas Monaco, Kari E. Veblen

All Current Publications

Our study supports using older naturally grown sagebrush plants (wildlings) for restoration efforts. Wildlings had already faced various environmental stresses before transplantation and then had a survival advantage when transplanted. Our study also demonstrates how successfully established transplants planted in “islands” aid in the future recruitment of sagebrush. Overall, because of their higher survival, we found that wildlings led to higher sagebrush densities and were a more cost-effective approach.

12.09.2024 Orsp Connect, Liz Williamson

ORED Newsletter

Employee Profiles

In Person Account Reconciliation and Tableau Training

NSF Proposals with Consultant or Professional Services

Deciphering Immune Suppression Mechanisms Of Primary And Metastatic Breast Cancer, Seunghyun Lee

Arts & Sciences Graduate Student Theses and Dissertations

Immune checkpoint blockade (ICB) has revolutionized cancer treatment, receiving FDA approval for various previously incurable cancers. In breast cancer, the combination of pembrolizumab (an anti-PD-1 antibody) with chemotherapy has become the standard-of-care for early-stage (Keynote-522) and advance-stage (Keynote-355) triple-negative breast cancer (TNBC). Unfortunately, over 30% of TNBC patients fail to respond to the combined chemo-immune therapy treatment, and the causes are unknown. Furthermore, ICB has demonstrated poor results in hormone-receptor positive breast cancer. While it is well-established that breast cancer cells can evade immune responses, the specific mechanisms driving immune evasion remain unclear. Thus, deciphering the immune system's role in …

Goose Grubbing And Warming Suppress Summer Net Ecosystem Co2 Uptake Differentially Across High-Arctic Tundra Habitats, Matteo Petit Bon, Karen H. Beard, Kari Anne Bråthen, Hanna Lee, Ingibjörg S. Jónsdóttir

Wildland Resources Faculty Publications

Environmental changes, such as climate warming and higher herbivory pressure, are altering the carbon balance of Arctic ecosystems; yet, how these drivers modify the carbon balance among different habitats remains uncertain. This hampers our ability to predict changes in the carbon sink strength of tundra ecosystems. We investigated how spring goose grubbing and summer warming—two key environmental-change drivers in the Arctic—alter CO₂ fluxes in three tundra habitats varying in soil moisture and plant-community composition. In a full-factorial experiment in high-Arctic Svalbard, we simulated grubbing and warming over two years and determined summer net ecosystem exchange (NEE) alongside its components: …

Agronomic Biofortification Of Calcium In Pak Choy (Brassica Rapa Subsp. Chinensis) Via Hydroponic Nutrient Film Technique, Regina Septiani Zahro, Khavid Faozi, Zulfa Ulinnuha, Agus Sarjito

Jurnal Kultivasi

Pak choy is rich in calcium, magnesium, phosphorus, iron, and vitamin K, all essential for health. Pak choy requires a proper balance of nutrients for optimal growth. In a hydroponic system, any imbalance in nutrient levels can negatively impact plant growth and reduce the nutritional value stored in the tissues. Selecting suitable varieties and providing appropriate calcium treatment can help increase calcium content and enhance productivity. This study aims to determine the effect of calcium concentration on growth, yield, and Ca content and the most responsive varieties to increased calcium concentration. The research design used was a Split Plot Design …

Oncologic Outcomes In Patients With Localized, Primary Head And Neck Synovial Sarcoma, Riddhi R Patel, Vancheswaran Gopalakrishnan, Behrang Amini, Alexander J Lazar, Patrick P Lin, Robert S Benjamin, Andrew J Bishop, Ryan P Goepfert, Dejka M Araujo

Faculty, Staff and Student Publications

Background: this study aims to evaluate the survival outcomes of patients suffering from head and neck synovial sarcoma (HNSS), especially in relation to patients with a localized disease at diagnosis.

Methods: this retrospective chart review includes 57 patients diagnosed with primary HNSS between 1981 and 2020 who presented with a localized disease at diagnosis. Overall survival (OS) from diagnosis, local recurrence-free survival (LRFS), and metastasis-free survival (MFS) from the end of the primary tumor treatment are estimated. The Kaplan-Meier method, the log-rank test, and the Cox proportional hazards regression are used.

Results: the 5-year OS, LRFS, and MFS are estimated …

Additional Expression Of T-Cell Engager In Clinically Tested Oncolytic Adeno-Immunotherapy Redirects Tumor-Infiltrated, Irrelevant T Cells Against Cancer Cells To Enhance Antitumor Immunity, Daisuke Morita, Amanda Rosewell Shaw, Greyson Biegert, Caroline Porter, Mae Woods, Spyridoula Vasileiou, Bora Lim, Masataka Suzuki

Faculty, Staff and Student Publications

Background: Oncolytic adenoviruses (OAds) are the most clinically tested viral vectors for solid tumors. However, most clinically tested "Armed" OAds show limited antitumor effects in patients with various solid tumors even with increased dosages and multiple injections. We developed a binary oncolytic/helper-dependent adenovirus system (CAdVEC), in which tumors are coinfected with an OAd and a non-replicating helper-dependent Ad (HDAd). We recently demonstrated that a single low-dose CAdVEC expressing interleukin-12, programmed death-ligand 1 blocker, and HSV thymidine kinase safety switch (CAdTrio) induces significant antitumor effects in patients, including complete response. Similar to previous OAd studies, all patients primarily amplified Ad-specific T …

Prediction Of Npk Doses Based On Targeted Fruit Sugar Content In Cucumis Melo L. ‘Cantaloupe’ Using A Simple Regression Method, Rangga Wibawa, Syariful Mubarok, Kusumiyati, Fiky Yulianto Wicaksono, Ruminta, Rahmat Budiarto, Dina Putri, Irvan Varinto, Muhammad Rizki, Adrian Maolana, Kahirun Nisa, Gunes Giyarto

Jurnal Kultivasi

The fruit sweetness is the main target in melon plant production. The highest criterion of sweetness is excellent, with 16% of total sugar content. Modification of essential plant nutrients is the alternative to reach that category. So, this study aims to obtain optimum NPK doses using a simple regression method. The experiment was conducted in a greenhouse with a soilless culture hydroponic system from August until November 2023 using a completely randomized design (CRD) with five treatments and four replications. The parameters included leaf area, plant dry matter, leaf nutrient uptake, fruit weight, and fruit sugar content. Pearson correlation analysis …

Potential Propagation Seedling Of Ramie (Boehmeria Nivea) From Various Types Of Stem Cuttings, Taufiq Hidayat Rahman Side, Mayasari Yamin, Sri Mulyani, Agung Pangestu Aji, Luthfi Ayunawati

Jurnal Kultivasi

Ramie is a fiber-producing plant that can replace cotton as a raw material for the textile industry. In general, ramie plants are reproduced using rhizomes, but it takes a long time, around two years, to be used as a source of planting material. Therefore, other sources of explants besides rhizomes are required, such as stem cuttings. Explants from stem cuttings are quickly available and only need 2-3 months to be used as planting material. This study aims to determine the potential of effective stem cuttings that can be used as planting material for the propagation of ramie seedlings. The research …

The Role Of Dna Topoisomerases In The Completion Of Dna Replication, Parisa Mokhtari

Dissertations and Theses

Accurate replication of the genome is essential to maintain cellular life. Completing DNA replication on the chromosome is central to this task and involves several enzymes, including RecBCD, RecG and SbcCD-ExoI, which process, join, and resolve the two convergent replication forks. This event is certain to generate a number of structural complexities involving supercoiling, tangles and knots as the two DNA replication forks converge which must be resolved to maintain the integrity of the genome. Structural knots and tangles generated during DNA replication, transcription and segregation are typically resolved by DNA topoisomerases. E. coli encodes four DNA topoisomerases--two type I …

Automated Segmentation Of Mri White Matter Hyperintensities In 8421 Patients With Acute Ischemic Stroke, Hosung Kim, Wi-Sun Ryu, Dawid Schellingerhout, Jonghyeok Park, Jinyong Chung, Sang-Wuk Jeong, Dong-Seok Gwak, Beom Joon Kim, Joon-Tae Kim, Keun-Sik Hong, Kyung Bok Lee, Tai Hwan Park, Jong-Moo Park, Kyusik Kang, Yong-Jin Cho, Byung-Chul Lee, Kyung-Ho Yu, Mi Sun Oh, Soo Joo Lee, Jae-Kwan Cha, Dae-Hyun Kim, Jun Lee, Man Seok Park, Hee-Joon Bae, Dong-Eog Kim

Faculty, Staff and Student Publications

Background and purpose: To date, only a few small studies have attempted deep learning-based automatic segmentation of white matter hyperintensity (WMH) lesions in patients with cerebral infarction; this issue is complicated because stroke-related lesions can obscure WMH borders. We developed and validated deep learning algorithms to segment WMH lesions accurately in patients with cerebral infarction using multisite data sets involving 8421 patients with acute ischemic stroke.

Materials and methods: We included 8421 patients with stroke from 9 centers in Korea. 2D UNet and squeeze-and-excitation (SE)-UNet models were trained using 2408 FLAIR MRIs from 3 hospitals and validated using 6013 FLAIR …

Antibiotic Resistance Of Laboratory E. Coli Strains, Alexis H. Hart

Honors Projects

Antibiotic resistance is an ongoing global health crisis. The prevalence and distribution of antibiotic resistance in environmental microbial communities have become climactic areas of investigation due to their far-reaching implications for ecosystem health, human well-being, and the sustainability of antimicrobial efficacy.

Transcription And 3d Chromatin Organization Interplay At Sub-Kilobase Scale, Hannah Harris

Theses & Dissertations

Nuclear compartments are prominent features of 3D chromatin organization, but sequencing depth limitations have impeded investigation at ultra fine-scale. CTCF loops are generally studied at a finer scale, but the impact of looping on proximal interactions remains enigmatic. Here, we critically examine nuclear compartments and CTCF loop-proximal interactions using a combination of in situ Hi-C at unparalleled depth, algorithm development, and biophysical modeling. Producing a large Hi-C map with 33 billion contacts in conjunction with an algorithm for performing principal component analysis on sparse, super massive matrices (POSSUMM), we resolve compartments to 500 bp. Our results demonstrate that essentially all …

Leveraging Social Ties To Financial Gains: Exploring The Impact Of Social Capital In Rural Development, Mallory L. Rahe, Andrew J. Van Leuven, Trey Malone

Department of Agricultural Economics Faculty Publications

Social capital, defined as networks of individuals linked through bonding or bridging relationships, plays a crucial but poorly conceptualized role in place-based development. The word “capital” implies an underlying value of the social connections throughout a community, and this study explores how these social relationships are used to initiate, plan, access financial capital, and implement economic development projects in rural areas. We avoid county-level aggregation bias and use a divergent pathway case study of projects across eight communities to examine how social ties are used during rural development in places with both high and low financial capital. Both types of …

Assessment Of The Accuracy Of A Deep Learning Algorithm- And Video-Based Motion Capture System In Estimating Snatch Kinematics, Federico Thiele, Florian K. Paternoster, Chris Hummel, Fabian Stöcker, Denis Holzer

International Journal of Exercise Science

International Journal of Exercise Science 17(1): 1629-1647, 2024. In weightlifting, quantitative kinematic analysis is essential for evaluating snatch performance. While marker-based (MB) approaches are commonly used, they are impractical for training or competitions. Markerless video-based (VB) systems utilizing deep learning-based pose estimation algorithms could address this issue. This study assessed the comparability and applicability of VB systems in obtaining snatch kinematics by comparing the outcomes to an MB reference system. 21 weightlifters (15 Male, 6 Female) performed 2–3 snatches at 65%, 75%, and 80% of their one-repetition maximum. Snatch kinematics were analyzed using an MB (Vicon Nexus) and VB (Contemplas …

Evaluating The Impact Of A One Day Resiliency Training Facilitation Program On Nurses' Wellbeing In An Acute Care Hospital, Amarachi Chidinma Eke-Okoro

Student Scholarly Projects

Practice Problem: Nursing burnout is a significant problem with far-reaching consequences. High levels of stress, emotional exhaustion, and reduced job satisfaction among nurses contribute to poor patient outcomes, increased medical errors, and high turnover rates. There is a high prevalence of burnout among nurse executives, consequences of which include extreme stress, emotional anguish, and a desire to quit, which points to a larger problem with burnout affecting the nursing profession (Martin et al., 2023). Fostering resilience among nurses can help to mitigate these outcomes by enhancing physical, mental, and social health through building purpose, positive emotions, and effective stress management. …

Correlation Of Arbuscular Mycorrhizal Dosage With Level Of Colonization, Nutrient Concentration, And Photosynthesis Pigment Of Cavendish Banana Plant, Muhammad Akhsan Akib, Nur Ilmi, Risnawati, Retno Prayudyaningsih, Syatrawati

Jurnal Kultivasi

The commercial development of Cavendish bananas still faces many obstacles, including the availability of seedlings/suckers at low prices, so technological substitution is needed to stimulate the growth of Cavendish banana suckers in the form of arbuscular mycorrhizal (AM) biological agents. The research aims to determine the correlation between AM doses and colonization levels, tissue nutrient concentrations, and photosynthetic pigments in Cavendish banana seedlings/suckers. It is also a novelty in this research study. The research was carried out in Parepare city at coordinates 3°59'30.204" S; 119°38'42.936" E using four AM doses as independent variables, namely 0 g, 5 g, 10 g, …

Metabolic Response Of The Code Goby (Gobiosoma Robustum) To Estuarine Climate Change Forecasts, Karli S. Corey

All HCAS Student Capstones, Theses, and Dissertations

Forage fishes are small bodied species that form a diverse functional group, serving as a primary food source for a wide range of marine predators. This group plays a significant ecological role through energy transfer to higher trophic levels. Gobiosoma robustum (Code Goby) is a substantive diet component of several recreationally significant juvenile sportfishes in Florida estuaries. While the thermal tolerance of this species may be inferred from observational data, the absolute physiological limitations of this species remain unknown. In this study, intermittent-flow respirometry and critical thermal maxima were used to determine the response of G. robustum to chronic and …

Spatial Ecology Of Large Bodied Ichthyofauna In The Red River Of The North Basin, William Marshall Stuart

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding spatial patterns and processes is essential for designing research studies, implementing management actions, and planning restoration efforts. The Red River of the North basin spans a broad geographic area where jurisdictional oversight is divided among several entities (Manitoba, CAN; North Dakota, USA; Minnesota, USA; South Dakota, USA), creating a mosaic of regulatory and functional process zones. Understanding the spatial ecology of freshwater fishes in this disjointed landscape is essential to address diverse life history requirements and the movement constraints imposed by anthropogenic structures. Long-term acoustic telemetry revealed that large bodied ichthyofauna employ a diverse range of movement strategies (i.e., …

Black Grouper (Mycteroperca Bonaci) Neurocranium, Nicholas Burchett, Kelsee Rotering, Marissa Rosas, David Kerstetter, Derek Burkholder

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Use Of Creative Outlets To Impact Quality Of Life For Individuals With Amyotrophic Lateral Sclerosis, Andria Burden, Helen Carey, Zeida Gutierrez

Fall 2024 OTD Capstone Symposium

Amyotrophic Lateral Sclerosis (ALS) profoundly impacts quality of life by limiting participation in meaningful activities. This mixed-methods study explored the role of creative outlets in enhancing quality of life for individuals with ALS. Quantitative data from the ALS-Specific Quality of Life Questionnaire-Short Form (ALSSQOL-SF) and qualitative insights from semi-structured interviews revealed key themes of adaptation, purpose, community, and quality of life. Findings indicate that creative outlets foster self-expression, social connection, and cognitive engagement while reducing isolation. Recommendations include integrating tailored creative activities into occupational therapy (OT), emphasizing group settings and early introduction to promote adaptability and community-building. This study underscores …

Going Vegan Can Progressively Lift Us Into Heightened Consciousness – Exclusive Book Extract From Hungry Beautiful Animals, Matthew C. Halteman

University Faculty Publications and Creative Works

In this excerpt taken from Chapter 5 of the new book Hungry Beautiful Animals, author and philosopher Matthew Halteman argues that choosing not to eat animals can usher us into a higher state of consciousness.

Comparative Study Of Rice Morphological And Physiological Characteristics Of Rice Grown Under Organic And Inorganic Farming, Amad Safrudin, Ratna Dewi, Dulbari

Jurnal Kultivasi

Organic farming practices have shown a potential to improve rice yields, aside from the popular conventional ones. This study aimed to comparatively analyze the morpho-physiological characteristics of rice plants grown under organic and inorganic farming. This research was conducted at the Polinela Organic Farm experimental station (5°21'10"S 105°13'43"E, 114 m sea above level), from February to April 2023 using a completely randomized design. The results showed that rice grown organically exhibited a notably higher chlorophyll index and actual water use efficiency compared to those grown inorganically. Conversely, inorganic farming resulted in a greater number of tillers than organic farming. However, …

Maize Hybrids’ Genetic Variability Based On Qualitative And Quantitative Traits, Slamet Bambang Priyanto, Herawati, Suwarti, Ayyub Ar Rahman, Nining Nurini Andayani, Abdul Fattah, Muhammad Azrai

Jurnal Kultivasi

Genetic variability was a prerequisite to doing a plant breeding program. A broad genetic variability allows plant breeders to select a desired genotype. This research aims to assess the maize hybrid's genetic variability based on qualitative and quantitative traits. This research was conducted in the Bone district, south Sulawesi, from November 2022 to March 2023. Fifteen maize hybrids were arranged in a randomized complete block design with three replications. The variables observed are qualitative and quantitative traits. Principal component (PCA) and cluster analyses assessed the genetic variability. The result indicated that based on a loading factor greater than 0.70, the …

Biological Control Of Roseau Cane Scale With Parasitoid Wasps In Louisiana, Tanner C. Sparks

LSU Doctoral Dissertations

Roseau cane scale, Nipponaclerda biwakoensis (Hemiptera: Aclerdidae) is an invasive species of scale insect negatively impacting Phragmites australis (Cyperales: Poaceae) stands within the Mississippi River Delta. The scales were first detected in 2017 from reports of Phragmites die-back within the MRD by landowners. Initial range surveys located RCS infested Phragmites stands were primarily limited to southeastern Louisiana with a single population near Port Arthur, Texas. Follow-up field surveys conducted annually from 2021 to 2023 monitoring further range expansion by the scale show the current range extends nearly 600 km between High Island, Texas and Bayou la Batre, Alabama. Climate matching …

Mutation- And Mrd-Informed Treatments For Transplant-Ineligible Patients, Curtis A Lachowiez, Courtney D Dinardo

Faculty, Staff and Student Publications

The ongoing development of molecularly targeted therapies in addition to the new standard of care combination of azacitidine and venetoclax (AZA-VEN) has transformed the prognostic outlook for older, transplant-ineligible patients with acute myeloid leukemia (AML). While conventional treatments, such as standard anthracycline and cytarabine- based chemotherapy or hypomethylating agent (HMA) monotherapy, are associated with a generally poor prognosis in this patient population, the use of these novel regimens can result in long-lasting, durable remissions in select patient subgroups. Furthermore, the simultaneous discovery of resistance mechanisms to targeted therapies and AZA-VEN has enabled the identification of patient subgroups with inferior outcomes, …