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Articles 18781 - 18810 of 255975

Full-Text Articles in Life Sciences

Jaguar (Panthera Onca) Population Status And Conservation Needs In Panama Revealed Using Spatial Density And Occupancy Models., Rebeca E. Becdach Jan 2025

Jaguar (Panthera Onca) Population Status And Conservation Needs In Panama Revealed Using Spatial Density And Occupancy Models., Rebeca E. Becdach

Cal Poly Humboldt theses and projects

Jaguars (Panthera onca) are key predators and the largest felid in the Americas. The species is listed as near threatened globally due to habitat loss, fragmentation, and human persecution. Their population decline can negatively affect the structure and functioning of the ecosystems they inhabit. Conservation efforts for jaguars and their habitat require understanding jaguar populations and how they are impacted by human presence across their range. This information is lacking in Panama, a critical portion of the Jaguar range. Panama forms a narrow land bridge connecting Central and South America, integral to wildlife movement, including jaguars, across both …

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Home Range, Habitat Use, And Fall Migration Of Lewis’S Woodpeckers In The Eastern Cascade Range, Oregon, Cameron Piper Jan 2025

Home Range, Habitat Use, And Fall Migration Of Lewis’S Woodpeckers In The Eastern Cascade Range, Oregon, Cameron Piper

Cal Poly Humboldt theses and projects

Lewis’s woodpecker (Melanerpes lewis), a burn specialist, is experiencing range-wide population declines despite increasing fire activity in the western U.S. The White River Wildlife Area in Oregon hosts the state's only year-round population, raising questions about their breeding, habitat use, and migration. In summer 2024, we tracked nine woodpeckers using telemetry to estimate home range size and overlap. Full home ranges averaged 428 ha, with 66 ha core areas, and showed evidence of growing after nesting. Home ranges overlapped extensively (Full: 91 ± 13%; Core: 63 ± 29%), though individuals had distinct areas of intense use. Males had …

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An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan Jan 2025

An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan

Cal Poly Humboldt theses and projects

Since the early 2010s, the seaweed aquaculture industry has steadily expanded in the United States, with states like Alaska, Maine, Hawaii, and Connecticut leading the way in the development of this emerging sector. However, information gaps remain concerning certain aspects of the domestic seaweed aquaculture industry. The purpose of this study was to examine the status of the seaweed aquaculture industry along the West Coast of the United States; specifically in the states of California, Oregon, and Washington. Seaweed farmers in these states were invited to voluntarily complete surveys asking important production, economic, and commercial questions regarding their operations. Data …

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Roosting Habits Of Male Silver-Haired Bats In The Willard Creek Drainage Of Northeastern California, Alexander Christopher Lewis Jan 2025

Roosting Habits Of Male Silver-Haired Bats In The Willard Creek Drainage Of Northeastern California, Alexander Christopher Lewis

Cal Poly Humboldt theses and projects

Silver-haired bats (Lasionycteris noctivagans) are one of the most widely distributed forest bats in North America. Although wildfires have been increasing across their range, how they respond to wildfire is understudied. Previous studies on silver-haired bats and fire focused on roosting behavior in low-severity controlled burns or used acoustics to understand activity levels post-wildfire. Quantifying how silver-haired bats use the physical structures created by high-severity wildfires and the landscape features in which they occur is critical in the face of rapidly shifting fire regimes. During the summer of 2023 and 2024 I radio-tracked male silver-haired bats to 37 roosts …

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Investigating Fish Communities As Part Of The Food Web In Artificial Tidal Wetland Ponds Of Humboldt Bay, Ca, Lily M. Olmo Jan 2025

Investigating Fish Communities As Part Of The Food Web In Artificial Tidal Wetland Ponds Of Humboldt Bay, Ca, Lily M. Olmo

Cal Poly Humboldt theses and projects

In the face of global wetland loss, artificially created wetlands have the potential to restore ecosystem services for humans while also providing habitat for fish and wildlife. This study explored fish communities in three understudied artificial wetland ponds that are connected to Humboldt Bay in northern California and known to be important wildlife foraging areas. The objectives of this research were to 1) Characterize the environmental conditions of each pond, 2) Calculate the relative catch, richness, diversity, and biomass of fish in each pond, 3) Describe the movement of fish into and out of the ponds with the tides, and …

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Elongate Locomotors Show Consistent Torque Patterns Across Substrates, Spencer Bradford, Henry Astley Jan 2025

Elongate Locomotors Show Consistent Torque Patterns Across Substrates, Spencer Bradford, Henry Astley

Williams Honors College, Honors Research Projects

The four main movements found in snakes are understood mainly due to external study; however, muscular forces and resulting torques are poorly known. The main research objective is to study the torque production on different substrates using a snakebot. The study will give a rudimentary model for torque production on different substrates. It can then be compared against biological systems to understand snake movement at a muscular level. This study used a snakebot, allowing direct assessment of the current being run through each of its motors (proportional to torque). Data was then examined using a Principal Component Analysis test for …

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Expression And Purification Of Cytochrome P450 46a1 For Resonance Raman Spectroscopy, Lauren Jirles Jan 2025

Expression And Purification Of Cytochrome P450 46a1 For Resonance Raman Spectroscopy, Lauren Jirles

Williams Honors College, Honors Research Projects

Researchers are targeting cytochrome P450 46A1 (CYP46A1), an enzyme critical for brain cholesterol homeostasis, as a potential therapeutic approach for neurological diseases. CYP46A1 plays a key role in cholesterol turnover in the brain by catalyzing the conversion of cholesterol to 24S-hydroxycholesterol (24HC) and is linked to Alzheimer’s disease pathophysiology. Given its critical role in brain health, CYP46A1 represents a promising target for therapeutic intervention in neurological disorders. The goal of this project was to express the CYP46A1 protein in GC5 E. coli cells and purify the protein using chromatographic techniques including affinity and ion exchange chromatography. Ultraviolet-Visible (UV-Vis) spectroscopy was …

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Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill Jan 2025

Effects Of Diet Composition On Physical Parameters In Danio Rerio, Isabella Hemphill

Williams Honors College, Honors Research Projects

With the use of zebrafish as a common model organism for human health, the reduction of confounding variables in their care is key. Many commercialized zebrafish diets are available for purchase, but each one consists of a different ratio of these crucial macronutrients. This study aimed to determine the effects long-term diets high in one macronutrient would have on the morphometrics, heart, liver, and protein content of zebrafish. Four diets were fed over a period of almost two years- high lipid, high carb, high protein, and a control diet. The control diet was the standard feed used in this lab, …

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Impact Of Tetrahydrocannabinol (Thc) On Daphnia Magna Heart Rate, Ashley Cosgrave Jan 2025

Impact Of Tetrahydrocannabinol (Thc) On Daphnia Magna Heart Rate, Ashley Cosgrave

Williams Honors College, Honors Research Projects

Marijuana (Cannabis) is currently federally illegal within the United States and classified as a Schedule I drug. This leaves a large research gap on the impacts of Tetrahydrocannabinol (THC) use. Daphnia magna are frequently used as a model organism for the human heart and toxicology screenings due to their myogenic heart, similarities in physiological pathways, and sensitivity to toxins. As such, in order to bridge the research gap and uncover potential use of Daphnia magna as a model organism for humans in regard to impact on heart rate, we hypothesized that if Daphnia magna possess CB1 receptors or an equivalent …

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Bat Species Diversity In The Klamath Basin Prior To Dam Removals, Ryan Page Matilton Jan 2025

Bat Species Diversity In The Klamath Basin Prior To Dam Removals, Ryan Page Matilton

Cal Poly Humboldt theses and projects

River ecosystems make up a small percentage of the earth’s surface, but they support high levels of biodiversity and attract high levels of human use. Dams, for example, disrupt a river’s natural course and flow, alter water temperatures in the stream, redirect river channels, transform floodplains, and fragment river continuity, which affects ecological balance and food webs. The Klamath River in northern California, which has been dammed for the last century, experienced dramatic disruptions including a large fish die-off in 2002. With the negative impacts of the Klamath dams apparent, multiple entities negotiated to remove the dams and restore the …

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Effects Of Experimental Structural Retention Position And Aggregation On Avian Communities In Managed Forests Of The Pacific Northwest, U.S., Aidan M. Healey Jan 2025

Effects Of Experimental Structural Retention Position And Aggregation On Avian Communities In Managed Forests Of The Pacific Northwest, U.S., Aidan M. Healey

Cal Poly Humboldt theses and projects

Managed forest lands in Oregon and Washington produce wood products and host diverse bird communities, though standing dead snags are often limited. State forest practices rules governing forest harvest dictate retention of a minimum number of trees when forests are clearcut harvested. However, little research has explored how the slope position and aggregation of retention, and the supplemental inclusion of mechanically created snags, affects use of these forest units by wildlife, especially highly mobile taxa such as birds. I used an avian point count sampling approach, within a replicate-block experiment dictating unit-level slope position, aggregation, and snag retention treatments, to …

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Associational Seaweed Defense Reduces Sea Urchin Grazing On Bull Kelp: Potential Use In Restoration, Brayden Tate Wiley Jan 2025

Associational Seaweed Defense Reduces Sea Urchin Grazing On Bull Kelp: Potential Use In Restoration, Brayden Tate Wiley

Cal Poly Humboldt theses and projects

Kelp forests are declining globally under warming seas, altered predator–prey dynamics, and intensified herbivory, with sea urchins often driving shifts from canopy-dominated forests to persistent urchin barrens. Management commonly relies on labor-intensive urchin removals, with variable success, underscoring the need for complementary, ecosystem-based tools that directly reduce urchin grazing pressure. Focusing on far northern California kelp forests, we combined subtidal surveys, field experiments, and laboratory feeding assays to assess whether the understory alga Desmarestia cf. herbacea can provide associational defense for bull kelp (Nereocystis luetkeana) by physically deterring purple sea urchin (Strongylocentrotus purpuratus) grazing and offering low …

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The Impact Of Guided Goal Setting On Mood And Exercise Performance In College-Age Active Individuals, Payton R. Heaney Jan 2025

The Impact Of Guided Goal Setting On Mood And Exercise Performance In College-Age Active Individuals, Payton R. Heaney

Cal Poly Humboldt theses and projects

Mental health challenges are common in college students and student athletes, yet finding accessible and effective methods to improve mood and mood disorders remains under-researched. Mood is closely related to perceived performance, making mood improvements even more important for active college individuals. This study examined whether guided goal setting and health coaching could significantly improve mood as well as perceived performance. Sleep and nutrition were targeted as key factors due to their well-researched connection to mood. Participants of this study were 25 healthy active college individuals aged 18-35. The sports and activities represented in this study were a mix of …

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Loss Of Lgl1 Increases Expression Of C-Jun And Dcx In The Svz And Rms Of The Mouse Brain, Madison Leigh Gutierrez Jan 2025

Loss Of Lgl1 Increases Expression Of C-Jun And Dcx In The Svz And Rms Of The Mouse Brain, Madison Leigh Gutierrez

Cal Poly Humboldt theses and projects

Glioblastoma (GBM), the most aggressive and treatment-resistant subtype of glioma, accounts for the majority of brain tumor–related mortality. A defining feature of GBM is its ability to invade healthy brain tissue through extensive cell migration, which severely limits the effectiveness of surgical and pharmacological treatments. Increasing evidence suggests that GBM cells activate gene programs known to drive normal neural stem and progenitor cell (NSPC) migration; however, the precise molecular mechanisms shared between NSPCs and GBM cells remain poorly understood. Lethal (2) giant larvae 1 (Lgl1), a conserved cortical cytoskeletal protein, has emerged as a key regulator of polarity and migration. …

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Cultivation Of Winged Kelp (Alaria Marginata) In Humboldt Bay, Evan C. Simpson Jan 2025

Cultivation Of Winged Kelp (Alaria Marginata) In Humboldt Bay, Evan C. Simpson

Cal Poly Humboldt theses and projects

The cultivation potential of the winged kelp (Alaria marginata) was evaluated in Humboldt Bay, California, to further develop sustainable aquaculture in the region. Seasonality, depth, and system design were evaluated in regard to harvest biomass and quality. Results suggested that single-longline configurations at shallow depths (< 2 m) produced greater total biomass than a multi-longline system, suggesting enhanced water flow and reduced self-shading improved growth. Kelp grew best with a winter outplanting period (December-April), most likely due to environmental conditions (e.g., temperature, nutrients, and salinity) being within the kelp’s tolerable range, with as much as 2.24 kg/meter of kelp being harvested. These findings demonstrate that while the estuarine system has more variable conditions than predicted, A. marginata can be successfully cultivated in Humboldt Bay and highlight its potential as a viable crop for northern California’s emerging seaweed industry. Future work targeting the optimization of single-longline design and deployment timing may further improve yield and support regional goals for sustainable aquaculture and food security.

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A Porpoise-Driven Approach: Using Agent-Based Models To Predict Population Consequences Of Offshore Wind Noises On Gulf Of Maine Harbor Porpoises, Ben Greenberg Pridonoff Jan 2025

A Porpoise-Driven Approach: Using Agent-Based Models To Predict Population Consequences Of Offshore Wind Noises On Gulf Of Maine Harbor Porpoises, Ben Greenberg Pridonoff

Cal Poly Humboldt theses and projects

Marine mammals are increasingly exposed to anthropogenic noises, including from offshore wind activities, necessitating the development and refinement of tools to predict the effects of noise on their populations. Agent-based models (ABMs) are an effective tool that can simulate realistic movements and behaviors of animals to quantify population consequences of disturbance. In this study, I used the Europe-based ABM ‘DEPONS’ to (1) evaluate its effectiveness in a new environment, the Gulf of Maine, USA/Bay of Fundy, Canada, and (2) develop estimates of the population dynamics of local harbor porpoises (Phocoena phocoena) interacting with expected noises from proposed floating …

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Every Publication Needs An Author Verified Tl;Dr Statement, Sarah Oerther, Daniel B. Oerther Jan 2025

Every Publication Needs An Author Verified Tl;Dr Statement, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

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Mxene-Based Materials For Enhanced Water Quality: Advances In Remediation Strategies, Ali Mohammad Amani, Milad Abbasi, Atena Najdian, Farzaneh Mohamadpour, Seyed Reza Kasaee, Hesam Kamyab, Shreeshivadasan Chelliapan, Mostafa Shafiee, Lobat Tayebi, Ahmad Vaez, Atefeh Najafian, Ehsan Vafa, Sareh Mosleh-Shirazi Jan 2025

Mxene-Based Materials For Enhanced Water Quality: Advances In Remediation Strategies, Ali Mohammad Amani, Milad Abbasi, Atena Najdian, Farzaneh Mohamadpour, Seyed Reza Kasaee, Hesam Kamyab, Shreeshivadasan Chelliapan, Mostafa Shafiee, Lobat Tayebi, Ahmad Vaez, Atefeh Najafian, Ehsan Vafa, Sareh Mosleh-Shirazi

Electrical & Computer Engineering Faculty Publications

Two-dimensional MXenes are promising candidates for water treatment because of their large surface area (e.g., exceeding 1000 m²/g for certain structures), high electrical conductivity (e.g., >1000 S/m), hydrophilicity, and chemical stability. Their strong sorption selectivity and effective reduction capacity, exemplified by heavy metal adsorption efficiencies exceeding 95 % in several studies, coupled with facile surface modification, make them suitable for removing diverse contaminants. Applications include the removal of heavy metals (e.g., achieving >90 % removal of Pb(II)), dye removal (e.g., demonstrating >80 % removal of methylene blue), and radioactive waste elimination. Furthermore, 3D MXene architecture exhibit enhanced performance in antibacterial …

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Cannabinoid-2 Receptor Activation Attenuates Sulfur Mustard Analog 2-Chloroethyl-Ethyl-Sulfide-Induced Acute Lung Injury In Mice, Gregory Nicholson, Nicholas Richards, Janette Lockett, My Boi Ly, Raj V. Nair, Woong-Ki Kim, K. Yaragudri Vinod, Nagaraja Nagre Jan 2025

Cannabinoid-2 Receptor Activation Attenuates Sulfur Mustard Analog 2-Chloroethyl-Ethyl-Sulfide-Induced Acute Lung Injury In Mice, Gregory Nicholson, Nicholas Richards, Janette Lockett, My Boi Ly, Raj V. Nair, Woong-Ki Kim, K. Yaragudri Vinod, Nagaraja Nagre

Department of Biomedical and Translational Sciences Faculty Publications

Background: Exposure to sulfur mustard (SM; 2,2′-dichlorodiethyl sulfide) causes toxicity in the human body, particularly the lungs. The molecular mechanisms of SM-induced lung damage are elusive, and no effective treatments exist. This study explores the anti-inflammatory potential of cannabinoid receptor 2 (CB2R) activation in mitigating acute lung injury (ALI) and inflammation induced by 2-chloroethyl ethyl sulfide (CEES), a structural analog of SM. Methods: C57BL/6J mice were exposed to CEES via intratracheal administration to model ALI. CB2R activation was achieved through the intraperitoneal administration of HU308, a selective synthetic agonist. ALI and inflammation were evaluated at 48 h post-exposure to CEES. …

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Phenotype Specific Nuclear Lamina Remodeling In Hipsc Derived Cardiomyocytes Bearing Tnnt2 Sarcomeric Variants, Isabella Leite Coscarella, Olalekan H. Usman, Lili Wang, Maicon Landim-Vieira, Lillian Wirstiuk, Amber N. Brown, Diego A. R. Zorio, Brian K. Washburn, Cynthia Vied, Bjorn C. Knollmann, Christopher Solís, Vitold E. Galkin, P. Bryant Chase, J. Travis Hinson, Jerome Irianto, Jose Renato Pinto Jan 2025

Phenotype Specific Nuclear Lamina Remodeling In Hipsc Derived Cardiomyocytes Bearing Tnnt2 Sarcomeric Variants, Isabella Leite Coscarella, Olalekan H. Usman, Lili Wang, Maicon Landim-Vieira, Lillian Wirstiuk, Amber N. Brown, Diego A. R. Zorio, Brian K. Washburn, Cynthia Vied, Bjorn C. Knollmann, Christopher Solís, Vitold E. Galkin, P. Bryant Chase, J. Travis Hinson, Jerome Irianto, Jose Renato Pinto

Department of Biomedical and Translational Sciences Faculty Publications

[Summary] Cardiomyocytes endure physical stress from the myocardium environment while generating their own mechanical strains. The force generated by sarcomeres is transmitted both longitudinally to adjacent sarcomeres and laterally to the cytoskeleton via intermediate filaments. This mechanical stimulus impacts other organelles, including the nucleus, thus playing a vital role in sensing and signaling nuclear adaptations. However, there is limited understanding of how changes in cardiac contractility affect nuclear mechanics. Here, we sought to investigate the effects of hyper- and hypo-contractility in nuclei of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) bearing TNNT2 pathogenic variants associated with hypertrophic (HCM) or dilated …

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Daf-12’S Effects On Lifespan In C. Elegans, Randhir S. Multani Jan 2025

Daf-12’S Effects On Lifespan In C. Elegans, Randhir S. Multani

Williams Honors College, Honors Research Projects

We tested the hypothesis that daf-12 has the ability to upregulate mir-84 and mir-241 in C. elegans. Rifampicin helps C. elegans live longer by reducing glycation and activating daf-16. It is not clear if daf-12 is needed for this to happen. Daf-16 turns on genes that promote a longer lifespan. Daf-12 increases mir-84 and mir-241 and helps daf-16 become active so it can turn on genes that relate to lifespan. C. elegans were divided into four groups: wild-type, wild-type with RIF, daf-12 RNAi, and daf-12 RNAi with RIF. Worms were incubated at 20°C. An LDH assay and protein assay …

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Monitoring Plastic Pollution Using Bioindicators: A Global Review And Recommendations For Marine Environments, Matthew S. Savoca, Neil Angelo Abreo, Andres H. Arias, Laura Baes, Matteo Baini, Elisa Bergami, Susanne Brander, C. Anela Choy, Illaria Corsi, Bavo De Witte, Camila Domit, Sarah Dudas, Emily M. Duncan, Claudia E. Fernández, Maria Cristina Fossi, Ostin Garcés-Ordóñez, Brendan J. Godley, Daniel Gonzalez-Parades, Victoria González Carman, Bonnie M. Hamilton, Britta Denise Hardesty, Sang Hee Hong, Shirel Kahane-Rapport, Lauren M. Kashiwabara, Mariana Baptista Lacerda, Guillermo Luna-Jorquera, Clara Manno, Sarah E. Nelms, Cristina Panti, Diego J. Pérez-Venegas, Christopher K. Pham, Jennifer F. Provencher, Sara Purca, Harunur Rashid, Yasmina Rodríguez, Conrad Sparks, Chengjun Sun, Martin Thiel, Catherine Tsangaris, Robson G. Santos Jan 2025

Monitoring Plastic Pollution Using Bioindicators: A Global Review And Recommendations For Marine Environments, Matthew S. Savoca, Neil Angelo Abreo, Andres H. Arias, Laura Baes, Matteo Baini, Elisa Bergami, Susanne Brander, C. Anela Choy, Illaria Corsi, Bavo De Witte, Camila Domit, Sarah Dudas, Emily M. Duncan, Claudia E. Fernández, Maria Cristina Fossi, Ostin Garcés-Ordóñez, Brendan J. Godley, Daniel Gonzalez-Parades, Victoria González Carman, Bonnie M. Hamilton, Britta Denise Hardesty, Sang Hee Hong, Shirel Kahane-Rapport, Lauren M. Kashiwabara, Mariana Baptista Lacerda, Guillermo Luna-Jorquera, Clara Manno, Sarah E. Nelms, Cristina Panti, Diego J. Pérez-Venegas, Christopher K. Pham, Jennifer F. Provencher, Sara Purca, Harunur Rashid, Yasmina Rodríguez, Conrad Sparks, Chengjun Sun, Martin Thiel, Catherine Tsangaris, Robson G. Santos

Biological Sciences Faculty Publications

Monitoring the movement of plastic into marine food webs is central to understanding and mitigating the plastic pollution crisis. Bioindicators have been a component of the environmental monitoring toolkit for decades, but how, where, and which bioindicators are used in long-term monitoring programs has not yet been assessed. Moreover, these programs have yet to be synthesized and evaluated globally. Doing so is imperative if we are to learn from these pioneering programs and expand on their efforts. We reviewed global monitoring programs using bioindicators that focus on plastic pollution and found 11 worldwide that met our definition of long-term monitoring. …

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Edge Effects Impact Blue Carbon Dynamics Across Coastal Ecotones In A Tropical Seascape, Erik S. Yando, Jahson B. Alemu I, Kiah Eng Lim, Taylor M. Sloey, Michiel Van Breugel, Natasha Bhatia, Daniel A. Friess Jan 2025

Edge Effects Impact Blue Carbon Dynamics Across Coastal Ecotones In A Tropical Seascape, Erik S. Yando, Jahson B. Alemu I, Kiah Eng Lim, Taylor M. Sloey, Michiel Van Breugel, Natasha Bhatia, Daniel A. Friess

Biological Sciences Faculty Publications

Coastal wetlands are important for their ability to regulate global climate through the sequestration and long-term storage of carbon. Accurate quantification of ecosystem-specific carbon dynamics (including sequestration, storage, and fluxes) is needed to develop accurate carbon budgets that inform climate change mitigation. Most work to quantify carbon dynamics either use subsampling in core habitats or benefit transfers to upscale values. While these approaches are valuable, our understanding of carbon dynamics across ecosystem transitions and overall heterogeneity remains a critical gap in coastal ecosystems as boundaries are not always clear. In this study, we established transects across both mangrove and seagrass …

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Caveolin-Mediated Endocytosis: Bacterial Pathogen Exploitation And Host-Pathogen Interaction, Dibyasri Barman, Rishi Drolia Jan 2025

Caveolin-Mediated Endocytosis: Bacterial Pathogen Exploitation And Host-Pathogen Interaction, Dibyasri Barman, Rishi Drolia

Biological Sciences Faculty Publications

Within mammalian cells, diverse endocytic mechanisms, including phagocytosis, pinocytosis, and receptor-mediated endocytosis, serve as gateways exploited by many bacterial pathogens and toxins. Among these, caveolae-mediated endocytosis is characterized by lipid-rich caveolae and dimeric caveolin proteins. Caveolae are specialized microdomains on cell surfaces that impact cell signaling. Caveolin proteins facilitate the creation of caveolae and have three members in vertebrates: caveolin-1, caveolin-2, and caveolin-3. Many bacterial pathogens hijack caveolin machinery to invade host cells. For example, the Gram-positive facultative model intracellular bacterial pathogen Listeria monocytogenes exploits caveolin-mediated endocytosis for efficient cellular entry, translocation across the intestinal barrier, and cell–cell spread. Caveolin …

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Cellular Responses Of Astrangia Poculata (Ellis And Solander, 1786) And Its Symbiont To Experimental Heat Stress, Tyler E. Harman, Daniel Barshis, Briana Hauff Salas, Kevin B. Strychar Jan 2025

Cellular Responses Of Astrangia Poculata (Ellis And Solander, 1786) And Its Symbiont To Experimental Heat Stress, Tyler E. Harman, Daniel Barshis, Briana Hauff Salas, Kevin B. Strychar

Biological Sciences Faculty Publications

Climate change has had devastating effects on tropical coral reefs; however, much less is known regarding how heat stress affects temperate coral. This research focuses on Astrangia poculata (Ellis and Solander, 1786) collected from Narragansett Bay, RI, during the summer and winter seasons and understanding the effect of experimental thermal extremes (i.e., 26 °C) on seasonally different populations. Photosynthetic efficiency (Fv/Fm), symbiont density (via an inverse relationship with pixel intensity), and oxidative stress via reactive oxygen species (ROS) concentrations were measured on symbiotic and aposymbiotic A. poculata. Higher Fv/Fm rates were observed in summer- vs. winter-collected corals (p ≤ …

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Impacts Of Tick Parasitism On The Rodent Gut Microbiome, Robert J. Brinkerhoff, Joshua Pandian, Meghan Leber, Isabella D. Hauser, Holly D. Gaff Jan 2025

Impacts Of Tick Parasitism On The Rodent Gut Microbiome, Robert J. Brinkerhoff, Joshua Pandian, Meghan Leber, Isabella D. Hauser, Holly D. Gaff

Biological Sciences Faculty Publications

Host microbiota may impact disease vector behavior and pathogen transmission, but little is known about associations between ectoparasites and microbial communities in wildlife reservoir species. We used Illumina metagenomic sequencing to explore the impacts of tick parasitism on the rodent fecal microbiome in both a field and laboratory setting. We found that tick parasitism on wild hosts was associated with variation in the fecal microbiota of both the white-footed deermouse, Peromyscus leucopus, and the southern cotton rat, Sigmodon hispidus. In a lab experiment, we detected significant changes to the fecal microbiome after experimental exposure to immature ticks in …

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The First Iucn Red List Of Cold-Water Corals Highlights Global Declines, Julia D. Sigwart, A. Louise Allcock, Renata Carolina Mikosz Arantes, Kelsey Archer Barnhill, Narissa Bax, Julia S. Beneti, Saskia Brix, Gudmundur Gudmundsson, Catherine S. Mcfadden, Severin A. Korfhage, Christi Linardich, Declan Morrissey, Pedro De Oliveira Nascimento, Bárbara De Moura Neves, Steinunn H. Ólafsdóttir, Stefán Ragnarsson, Kaveh Samimi-Namin, Íris Sampaio, Mark E. De Wilt Jan 2025

The First Iucn Red List Of Cold-Water Corals Highlights Global Declines, Julia D. Sigwart, A. Louise Allcock, Renata Carolina Mikosz Arantes, Kelsey Archer Barnhill, Narissa Bax, Julia S. Beneti, Saskia Brix, Gudmundur Gudmundsson, Catherine S. Mcfadden, Severin A. Korfhage, Christi Linardich, Declan Morrissey, Pedro De Oliveira Nascimento, Bárbara De Moura Neves, Steinunn H. Ólafsdóttir, Stefán Ragnarsson, Kaveh Samimi-Namin, Íris Sampaio, Mark E. De Wilt

Biological Sciences Faculty Publications

The most well-known species-based conservation tool is the International Union for the Conservation of Nature (IUCN) Red List of Threatened Species. The current coverage of species in the Red List is known to under-represent benthic marine species. Cold-water corals (CWCs) are increasingly recognised as key to deep-water biodiversity and integral to protected vulnerable marine ecosystems (VMEs), but no deep-sea coral species were previously included in the Red List. We selected 22 cold-water coral species in the Northeast Atlantic, including 4 reef-forming stony corals and 18 octocorals including sea pens and gorgonians, and completed the first IUCN Red List global assessments …

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Dengue Virus Modulates Critical Cell Cycle Regulatory Proteins In Human Megakaryocyte Cells, Swarnendu Basak, Shovan Dutta, Supreet Khanal, Girish Neelakanta, Hameeda Sultana Jan 2025

Dengue Virus Modulates Critical Cell Cycle Regulatory Proteins In Human Megakaryocyte Cells, Swarnendu Basak, Shovan Dutta, Supreet Khanal, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

Suppression of human megakaryocytes by dengue virus (DENV) infection significantly reduces the platelet count that eventually leads to thrombocytopenia, severe dengue and death. To understand DENV interactions with megakaryocytes, we investigated the cell cycle in leukemic human megakaryocytic in vitro cell line (MEG-01 cells). Megakaryocytes are known for complex endomitotic cell cycle leading to their polyploidy state. Our study shows that DENV uses these polyploid cells for its replication. Understanding the modulation of DENV-mediated cell cycle regulation in megakaryocytes is therefore highly important. We show that DENV2 (serotype 2) infection significantly modulates cell cycle signaling. Our protein profile microarray data …

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Addressing The Productivity-Susceptibility Analysis In Data-Limited Fisheries For Understudied Fish In The Marine Aquarium Trade, Alice Wynn, Jemelyn Baldisimo, Gabrielle Baillargeon, Michael Tlusty, Andrew Rhyne Jan 2025

Addressing The Productivity-Susceptibility Analysis In Data-Limited Fisheries For Understudied Fish In The Marine Aquarium Trade, Alice Wynn, Jemelyn Baldisimo, Gabrielle Baillargeon, Michael Tlusty, Andrew Rhyne

Biological Sciences Faculty Publications

The marine aquarium trade (MAT) harvests millions of coral reef fishes from the wild yearly. While the MAT supports fishers, hobbyists and marine education, harvested fishes are crucial in maintaining coral reef health and balanced marine cycles within their native habitats. Understanding the impact of the MAT on fish populations is critical for sustaining the trade in the long-term. Productivity-susceptibility analyses (PSAs) offer semiquantitative methods of analyzing the productive capacity of each fish stock, as well as its susceptibility to overfishing. The present study revises and expands upon a PSA conducted by Baillargeon et al. (2020) that focused on 32 …

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Divergent Floral And Vegetative Trait Responses To Abiotic Factors Drive Phenotypic Diversity In Platanthera Dilatata (Orchidaceae) Across Alaska, Marlin L. Bowles, Lisa E. Wallace Jan 2025

Divergent Floral And Vegetative Trait Responses To Abiotic Factors Drive Phenotypic Diversity In Platanthera Dilatata (Orchidaceae) Across Alaska, Marlin L. Bowles, Lisa E. Wallace

Biological Sciences Faculty Publications

Multiple traits make up an integrated phenotype, but traits may be shaped by differing drivers. It is often assumed that selection by biotic agents drives floral variation, but abiotic factors are also known to influence floral traits. Understanding trait covariations and their association with diverse environmental factors is a central question in evolution and ecology. Here, we studied covariation in floral and vegetative traits in relation to climate and topo-edaphic variation in the orchid Platanthera dilatata. In Platanthera, nectar spur length is associated with pollinator tongue length, but much variation exists in other traits too, likely to be …

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