The Intermediate And Remote Effects Of Acute Juvenile Stress Upon Auditory Fear Learning, Recognition Memory, And Anxiety Measures In A Rodent Model Of Ptsd, Laura J. Baron

Electronic Theses & Dissertations (2024 - present)

Abstract

Early-life stress is a risk factor for increased susceptibility to stress- and trauma-related disorders, including Posttraumatic Stress Disorder (PTSD). PTSD is characterized by exposure to trauma that results in exaggerated and extinction-resistant fear responses that persist for over a month. Understanding how the severity of early-life stressors in animal models impacts adult behavior, cognition, and underlying neurobiology could aid in the development of more effective PTSD treatments.

Various early-life stress models, especially in infant rats, have been used to induce stress and assess PTSD like-symptoms. Different stressors impact short- and long-term behavior, with some effects influenced by developmental timing …

The Multifaceted Role Of Glutamate At Excitatory And Inhibitory Synapses, Maurice A. Petroccione

Electronic Theses & Dissertations (2024 - present)

Our thoughts, memories, and actions are finely controlled by the coordinated activity of neural circuits in the brain. Prior work has identified a multitude of molecular mechanisms that contribute to the generation of these higher order functions, yet we know very little about how our ability to learn and perform habitual actions change depending on how far neurotransmitters released from neurons can travel away from their point of release. This is the main focus of my work, which I carried out using a diverse toolbox and focusing on two brain regions: the hippocampus and the striatum. The hippocampus controls learning …

Unveiling The Cell Composition Of The Olfactory Migratory Mass: Perspectives On Terminal Nerve Formation, Olfactory Development, And Migration Of The Gnrh-1 Neurons, Enrico Amato Jr.

Electronic Theses & Dissertations (2024 - present)

Puberty is a postnatal developmental process required to initiate key sexual maturation events that are necessary for the propagation of species. Signals to initiate these events first begin with the Gonadotropin releasing hormone-1 (GnRH-1) neurons within the hypothalamus. The hormone GnRH-1 released by these neurons is crucial for puberty, fertility and reproductive processes by establishing the hypothalamic-pituitary-gonadal (HPG) axis, regulating release of sex hormones (Progesterone, Estrogen, Testosterone). Conditions related to inability of the GnRH-1 neurons to correctly release GnRH-1 can lead to aberrant or delayed puberty, a condition known as hypogonadotropic hypogonadism (HH). Interestingly, some patients with the hormonal disorder …

System And Method For Simulating A Respiratory System, Yang Wang, Weixing Hao, Yue-Wern Huang

Patents

A system and method for simulating a human respiratory system to facilitate investigating the functioning of the respiratory system, such as the generation and effects of secondhand smoke. An aerosol generator, which may include an atomizer, is configured to generate an aerosol. A series of filter media includes, in order, an inhaling extrathoracic filter media, an inhaling tracheobronchial filter media, an alveolar filter media, an exhaling tracheobronchial filter media, and an exhaling extrathoracic filter media. Each filter media includes one or more layers of a material having a fiber diameter and a weight that simulate the corresponding region of the …

Challenges And Lessons From A Vector Control Campaign Targeting Glossina Palpalis Palpalis In An Isolated Protected Forest Area In Abidjan, Côte D’Ivoire, Yao Jean Rodrigue Konan, Bi Tra Dieudonné Ta, Djakaridja Berté, Bamoro Coulibaly, Kinifo Donatien Coulibaly, Nick Steven Egnankon, Foungniguée Diarrassouba, Kouassi Albert Djabo, Stéphanie Watier-Grillot, Jean Paul Demoncheaux, Koffi Alain De Marie Kouadio, Louis N’Dri, Philippe Solano, Sophie Ravel, Guy Pacôme Adingra, Antoine Barreaux, Adeline Ségard, Dramane Kaba, Vincent Jamonneau, Thierry De Meeûs, Vincent Djohan

All Peer-Reviewed Publications

Vector control (VC) is one of the strategies employed to manage African trypanosomoses. This study aimed at assessing the effectiveness of a VC campaign against Glossina palpalis palpalis using tiny targets (TTs) impregnated with insecticide in an isolated, protected forest in Abidjan, Côte d’Ivoire, while considering ecological, genetic, and operational factors. Between January 2020 and September 2022, 2,712 TTs were deployed at 684 sites, covering a total area of 1.7 km2. VC monitoring was conducted using Vavoua traps during 12 evaluation surveys, between June 2020 and March 2023. Five months after the initial TT deployment, tsetse fly density had decreased …

Unraveling The Potential Of Black Soldier Fly Larvae As A Sustainable Protein Source For Nile Tilapia Production In Diverse Aquaculture Systems, Jonathan Munguti, Mavindu Muthoka, Jimmy B. Mboya, Domitila Kyule, Menaga Meenakshisundaram, Chrysantus M. Tanga

All Peer-Reviewed Publications

Aquaculture plays a critical role in global food security, with Nile tilapia (Oreochromis niloticus) recognized for its adaptability and robust growth. However, traditional feeds, heavily reliant on fishmeal (FM) and soybean meal, face economic and environmental challenges. In response, black soldier fly larvae meal (BSFLM) has emerged as a promising, nutrient-dense alternative. This review synthesizes existing literature on BSFLM’s nutritional profile and its suitability for Nile tilapia diets, while acknowledging that the data come from diverse independent studies conducted under varying environmental conditions and husbandry practices. BSFLM consistently provides high-quality protein (29.9%–48.2%), aligning with tilapia’s requirements, and is rich in …

Processed Amaranth And Finger Millet Enriched With Cricket Flour Influence The Functionality, Microbial Quality, Shelf Life, And Consumer Preference Of The Porridge Products, Nelly Maiyo, Michael Okoth, George Abong, Brian O. Ochieng, Fathiya Khamis, Margaret Kababu, Chrysantus M. Tanga

All Peer-Reviewed Publications

Exploration of nutritious, cheap, and easy-to-prepare locally available complementary foods using different processing techniques is gaining traction globally. This study evaluated the impact of three processing techniques (roasting, germination, and fermentation) on finger millet and amaranth quality. The implications of blending processed products with 10% cricket flour on the functional properties, microbial safety, and shelf life of the porridge products were tested. Thereafter, consumer preference and acceptability of the cooked porridge products were assessed with randomly enrolled panelist of mothers (20–66 years), as primary caregivers. Our results showed that powder from germinated cereal products had higher swelling power, water absorption …

Annual Diversity Of Honey Bee Pollen Sources In Two Pumpkin Growing Landscapes, Machakos County, Kenya, Marystella W. Nang’Oni, Muo Kasina, Rebecca Karanja, Mary M. Guantai, Rahab N. Kinyanjui, Evanson R. Omuse, Michael H.G. Lattorff, Elfatih M. Abdel-Rahman, Marian Adan, Samira A. Mohamed, Thomas Dubois

All Peer-Reviewed Publications

Multi-floral foraging sources for honey bee (Apis mellifera L.) have been threatened by landscape changes and unsustainable farming practices. In East Africa, the biodiversity of forage resources that could support honey bees, especially in agricultural lands, remains least explored. This study investigated pollen diversity for honey bees in Yatta and Masinga Sub-counties in Machakos County, Kenya. Honey bee hives were installed on eight pumpkin (Cucurbita maxima Duchesne ex Lam) farms (one hive per farm) in two varying landscape vegetation classes (low and medium) based on normalized difference vegetation index (NDVI). Pollen traps were installed at the hive entrance and pollen …

Contribution Of Different Frass Fertilizer Products On Enhanced Growth, Yield And Nutrient Quality Of Broccoli [Brassica Oleracea], Noella Kagehi, Dennis Beesigamukama, Chrysantus M. Tanga, Mathew P. Ngugi, Sevgan Subramanian, Fathiya M. Khamis

All Peer-Reviewed Publications

Vegetables are crucial for food security and income, but in developing countries their production is hindered by low soil fertility. Although the insect frass fertilizer is a potential solution, its use is constrained by limited product choices. Unlike conventional fertilizers, which are available in different forms, the insect frass fertilizer is mostly available in solid form. Here, we evaluated the effects of different black soldier fly frass fertilizer (BSFFF) products on broccoli [Brassica oleracea] growth, yield, and nutritional profiles. Solid, liquid BSFFF, chitin-fortified solid BSFFF, chitin-fortified liquid BSFFF, and commercial organic fertilizer (Safi) were applied at rates equivalent to 250 …

Insect Biological Control: A Global Perspective, Lessando M. Gontijo, Jorge B. Torres, Paul K. Abram, Armando Alfaro-Tapia, Hugo C. Arredondo-Bernal, Antonio Biondi, Raymond A. Cloyd, Alejandro C. Costamagna, Nicolas Desneux, Marie D’Ottavio, Michael J. Furlong, Nancy M. Greco, Roselyne Labbe, Martin Hill, Blas Lavandero, Tian Hao Li, José R. Lomelí-Flores, Eric Lucas, Gerben J. Messelink, Margarita Rocca, Marcela Rodriguez-Garcia, José R.P. Parra, Ainara Peñalver-Cruz, Bonoukpoè Mawuko Sokame, Sean Thackeray, Alberto Urbaneja, German Vargas, Myron P. Zalucki, Lian Sheng Zang

All Peer-Reviewed Publications

The intentional use of biological control agents to manage arthropod pests dates back to as early as 304 AD, when Chinese farmers used weaver ants to manage citrus pests. Over time, biological control has evolved into distinct approaches: classical, augmentation, and conservation. Prior to the advent of pesticides, most insect pest management relied on natural and classical biological control. The most common strategy used at first, despite regional variations, was the importation of biological control agents. Nonetheless, due to issues with unintentional non-target impacts, numerous failures in the introduction, and longer periods required for implementation, augmentative and conservation approaches have …

Behavioral Responses To Underwater Noise, Christine Erbe, Capri Jolliffe, Renee Schoeman, Valeria Senigaglia, Cristina Tollefsen, Rebecca Wellard, Ann Bowles, Dorian Houser, Shyam Madhusudhana, Sarah A. Marley, Angela Recalde Salas, Chandra Salgado-Kent, Leah Trigg

Research outputs 2022 to 2026

This chapter presents an overview of physical and acoustic behavioral responses of marine mammals to underwater sounds. A literature review was undertaken, and data on received levels at the animal when certain types of responses were observed were compiled in an online supplementary spreadsheet. Based on this, an overview of responses was written, organized first by species and then by sound type. In-air and underwater sound sources were considered. The most studied sound types were mid-frequency sonar and acoustic deterrent devices and then impact pile driving and vessels. The most frequently reported response was avoidance, followed by changes in swim …

Developing A Ninhydrin Assay To Determine Methionine And Lysine Loss From Rumen Protected Amino Acid Supplements, Ashley K. Miske

Honors Theses and Capstones

No abstract provided.

Using Rna Sequencing To Examine Encephalic Gene Expression In Cactus Mice, Sarah A. Nicholls

Honors Theses and Capstones

Many of the exact mechanisms by which desert-adapted animals avoid dehydration-induced complications are unknown, though there are likely ties to the general mammalian dehydration response, a multisystem process spearheaded by the brain. Peromyscus eremicus, the cactus mouse, can survive its whole life without intaking water and is commonly used as a model to study desert adaptations. In this study, we have used bulk transcriptomics to characterize the differential gene expression across hydrated and dehydrated P. eremicus in five major regions of the brain: the cerebral cortex (referred to throughout much of this paper as the cortex), hippocampus, thalamus, midbrain, …

Targeting Stat3 In Tnbc Drug Resistance, Madison A. Henry

Honors Theses and Capstones

Triple negative breast cancer (TNBC) is one of the most aggressive types of breast cancer and lacks the three commonly targeted receptors in breast cancer treatments. As a result of a lack of targetable receptors, the standard of care remains to be harsh chemotherapy treatments that often are successful in early TNBC cases but often result in relapsed TNBC. In addition to high rates of recurrence, TNBC is known for its aggressive metastasis and chemoresistance. Within TNBC, the transcription factor Signal Transducer and Activator of Transcription 3 (STAT3) is constitutively active and increases proliferation, and metastasis, and plays a role …

Optimization Of Small-Scale Bioreactors For Increased Pdna Production And Yield, Laura M. Hills

Honors Theses and Capstones

No abstract provided.

Pathogens And Parasites Of Feral And Managed Honey Bee, Apis Mellifera, Colonies From Oklahoma, Allen Szalanski

Entomology and Plant Pathology Faculty Publications and Presentations

Managed honey bee populations in the United States have been under stress over the past several decades, probably due to the interaction among multiple biotic and abiotic stressors. While most research has focused on national surveys of honey bee pathogens and parasites, state-specific studies that include both managed and feral honey bee populations may offer additional insights into the factors that contribute to the decline of honey bees. We conducted a survey to detect parasites and pathogens from feral and managed honey bees in colonies in Oklahoma. A total of 253 (89 managed and 164 feral) honey bees from these …

Ephemeral Seagrass Meadow Assessment To Improve Understanding Of Resilience Of Ephemeral Seagrasses On Kwinana Shelf For Environmental Impact Assessment And Management, Kathryn Mcmahon, Nicole Said, Chanelle Webster, H. Williams, Simone Strydom

Research outputs 2022 to 2026

Executive Summary:

• This study was conducted to generate knowledge to inform the environmental impact and prediction process for seagrass species that form ephemeral meadows in Cockburn Sound and Owen Anchorage.

• Local light thresholds for the opportunistic species, Syringodium isoetifolium were generated at summer and winter temperatures, with higher light requirements in summer compared to winter, and compared to larger, more slow-growing persistent species such as Posidonia sinuosa. The half-saturating irradiance (Ik) was 115 μmol m-2 s-1 at 23 °C.

• Based on the dynamics at three sites from December 2023 to April 2024, Halophila ovalis forms ephemeral meadows …

Genomic And Metabolic Network Properties In Thermophiles And Psychrophiles Compared To Mesophiles, Amal Saeed Alblooshi

Theses

Thermophiles and psychrophiles are extremophilic microorganisms that thrive in harsh conditions. Thermophiles are mostly located in hot springs, prosper in high temperatures. Psychrophiles thrive in extremely cold environments, including polar regions and deep-sea habitats. Although they play a crucial role in biotechnology and environmental research, their mechanisms of adaptation are still not well understood. The main objective of this thesis is to understand the genomic characteristics and metabolic network features in thermophiles and psychrophiles compared to mesophiles. The genomes of 59 species among thermophiles, psychrophiles, and mesophiles were collected from the NCBI database. These genomes were used to analyze their …

Evaluation Of The Corrosion Of Metallic Fastener Joints In Composite Materials Immersed In Seawater, Chloe Derrico

Electronic Theses and Dissertations 2020 - Present

The corrosion behavior of metallic fasteners in contact with composite materials in marine environments remains a critical concern for structural integrity and long-term performance. This study evaluates the corrosion of marine fasteners of various materials in contact with glass fiber, carbon fiber, hybrid glass-carbon fiber, and G10/FR-4 Garolite composite plates. The research focuses on localized corrosion mechanisms, include crevice and pitting corrosion, as well as the influence of composite interactions. Samples were exposed to three environmental conditions: full immersion tanks, tidal tanks simulating the high and low tides, and deployment on a barge on Florida’s intracoastal waterway, with antifouling paint …

Gadd34 And Crep Regulation In The Unfolded Protein Response, Comparing Molecular Mechanisms In Humans And Zebrafish, Angela Adams

University of the Pacific Theses and Dissertations

In eukaryotic cells, many newly synthesized proteins are sent to the endoplasmic reticulum (ER) of a cell to be folded into the proper conformation for functionality. When proteins get misfolded, or cannot be folded fast enough, these proteins accumulate in the ER which causes the cell to become stressed. This leads to a process known as the Unfolded Protein Response (UPR). Once UPR has been initiated, the α subunit of eukaryotic initiation factor 2 (eIF2α) becomes phosphorylated to inhibit global protein synthesis within the cell, while simultaneously activating the expression of several genes to either aid in cell recovery or …

Associations Between Dietary Sodium And Potassium Intakes, Urinary Sodium And Potassium Excretion And Blood Pressure Among Adult Females In Oromia Region, Ethiopia, Fred Baffour Addo

Open Access Master's Theses

Introduction: Accurate assessment of sodium (Na) and potassium (K) intake is essential for population level monitoring of salt intake as part of global strategies to reduce salt consumption to control the increasing prevalence of high blood pressure (BP). Thus, a simple, cost-effective, and low-burden method is particularly valuable in resource-limited settings for population level monitoring of Na and K intake. This study aimed to compare the reliability of various methods of Na and K intake assessment, and their associations with blood pressure measurements.

Methods: This secondary analysis was based on a cross-sectional study of 100 non-pregnant women of …

Bird Watching In Chitral, Sehba Akhter

Institute for Educational Development Pakistan (IED-PK) Archives

This booklet comprises brief information about different species of thrush birds found in Chitral, identified by different researchers and field guides. The sources used include Helm Field Guide, Birds of Pakistan by Richard Grimmett, Tom Roberts, Tim Inskipp, and The Birds of Pakistan by T.J Roberts. This booklet aims to help the people of Chitral know about the birds they see every day.

Isolation And Molecular Identification Of Pathogens Causing Sea Turtle Egg Fusariosis In Key Nesting Beaches In Costa Rica, Keilor E. Cordero-Umaña, Ruth Hernando-Martínez, María Martínez-Ríos, Jaime Restrepo, Roldán A. Valverde, Laura Martín-Torrijos, Pilar Santidrián Tomillo, Javier Diéguez-Uribeondo

School of Earth, Environmental, & Marine Sciences Faculty Publications

The global rise of fungal pathogens presents an emerging threat to biodiversity, with significant risks to species such as endangered sea turtles. The fungal disease known as sea turtle egg fusariosis (STEF) is associated with high embryo mortality rates and represents a substantial conservation challenge. This disease is caused by two fungal species, namely Fusarium falciforme (Ff) and Fusarium keratoplasticum (Fk), and their identification is essential for guiding future efforts to address potential fungal infections, particularly on important nesting beaches such as those in Costa Rica. In this study, we conducted fungal isolations from sea turtle eggshells …

Copula-Based Bayesian Model For Detecting Differential Gene Expression, Prasansha Liyanaarachchi, N. Rao Chaganty

Mathematics & Statistics Faculty Publications

Deoxyribonucleic acid, more commonly known as DNA, is a fundamental genetic material in all living organisms, containing thousands of genes, but only a subset exhibit differential expression and play a crucial role in diseases. Microarray technology has revolutionized the study of gene expression, with two primary types available for expression analysis: spotted cDNA arrays and oligonucleotide arrays. This research focuses on the statistical analysis of data from spotted cDNA microarrays. Numerous models have been developed to identify differentially expressed genes based on the red and green fluorescence intensities measured using these arrays. We propose a novel approach using a Gaussian …

Factors Affecting In-Hospital Mortality After Sudden Cardiac Arrest In The Emergency Department: A Retrospective Cohort Study, Erkan Boğa, Abdullah Algın

Mansoura Medical Journal

Giriş: Ani kalp hastalıkları (SCA), özellikle acil serviste (AS) yüksek hastane içi ölüm tutarları ve önemli bir tıbbi acil durumları. Prognostik bilgilerin belirlenmesi, sonuçların değiştirilmesi için çok önemlidir. Amaç: Acil serviste SCA'lı hastane içi ölümle sağlanan klinik ve demografik oranların belirlenmesi. Yöntemler: Bu retrospektif kohort çalışmaları Ocak 2023 ile Aralık 2024 arasında üçüncü basamaktaki bir hastanenin AS'sinde SCA'nın 345 yıl boyunca geçirdiği süre dahil edildi. Demografik özellikler, türü olmayan yayılma, durma özellikleri, resüsitasyon süresi, laboratuvar gelişimi ve patoloji durumuyla ilgili veriler toplandı. Bağımsız ölüm öngörmelerini belirlemek için lojistik regresyon kaydı. Sonuçlar: Hastane içi ölüm %38,3 idi. Çok değişkenli analiz, resüsitasyon …

Clinical And Radiological Outcomes Of Posterior Malleolar Fractures Fixation Through Posterolateral Approach: A Prospective Case Series Study, Mohammed Abdulrafei Farag, Ahmed Elsayed Elhawary, Ahmed Salah-Eldeen Zaghloul

Mansoura Medical Journal

Background: Posterior malleolar fractures are frequently associated with other malleolar injuries and significantly affect ankle stability through the posterior tibiofibular ligament. CT imaging is vital for preoperative classification, fragment size measurement, and syndesmotic evaluation. Objective: To assess clinical and radiological outcomes of posterior malleolar fractures treated using the posterolateral approach. Methods: This prospective case series included 20 skeletally mature patients treated between March 2022 and January 2024. Preoperative evaluation included radiographs and CT scans for classification, fragment size, articular step-off, and syndesmotic injury. Postoperative reduction and alignment were assessed with X-ray and CT. Functional outcomes were evaluated at ≥6 months …

The Relationship Between Nutrition Knowledge And Dietary Behavior Of Student-Athletes, Kerry A. Smith, Kathleen Fritch, James Rost, Amy Jo Riggs, Juliann Sergi Mcbrayer, Monika Krah

Georgia Educational Researcher

The purpose of this study was to examine the relationship between nutrition knowledge and dietary behavior while controlling for academic major, year, and gender among Division III student-athletes. Sufficient nutrition is essential for athletic performance, injury reduction, and overall health. However, student-athletes face unique challenges that can disrupt optimal nutrition, including poor nutrition knowledge and access to available resources. While several studies have examined the relationship between nutrition knowledge and dietary behavior, no known studies have addressed this among Division III student-athletes. This non-experimental correlational study examined dietary behavior using the Rapid Eating and Activity Assessment for Participants (REAP-S) and …

Practical Experimental Microbiology: Laboratory Manual, Rivka Levron

Open Touro Created

2025

Microbiology, especially the laboratory component, is a highly practical and useful course for students pursuing many career options. While a number of excellent microbiology laboratory manuals are available, many are more suitable for a full-year course in microbiology or for more advanced studies.

Microbiology Experimental Laboratory Manual is comprehensive in teaching students basic microbiological techniques, i.e., how to grow and stain bacteria, how to identify bacteria and fungi, and means of identifying different species of bacteria microscopically. Further experiments enable students to identify the most effective soap/detergent from those readily available; to discover which spices have the most anti-bacterial …

Swatting Flies: Biting Insects As Non-Invasive Samplers For Mammalian Population Genomics, Anubhab Khan, Ryan Carter, Chengetai D. Mpamhanga, Daniel Masiga, Manun Channumsin, Marc Ciosi, Oliva Manangwa, Furaha Mramba, Umer Zeeshan Ijaz, Harriet Auty, Barbara K. Mable

All Peer-Reviewed Publications

Advances in next-generation sequencing have allowed the use of DNA obtained from unusual sources for wildlife studies. However, these samples have been used predominantly to sequence mitochondrial DNA for species identification while population genetics analyses have been rare. Since next-generation sequencing allows indiscriminate detection of all DNA fragments in a sample, technically it should be possible to sequence whole genomes of animals from environmental samples. Here we used a blood-feeding insect, tsetse fly, to target whole genome sequences of wild animals. Using pools of flies, we compared the ability to recover genomic data from hosts using the short-read sequencing (Illumina) …

Aflatoxin Prevalence And Association With Maize Grain Physical And Nutritional Qualities In The Informal Markets In Meru County, Kenya, C. Cherotich, M. W. Okoth, Abong’ Go, S. W. Kariuki, E. R. Omuse

All Peer-Reviewed Publications

In Kenya, aflatoxin contamination in maize remains a persistent issue, with potential negative impacts on both public health and economic growth. Aflatoxins are toxic secondary metabolites produced by certain mold species, primarily Aspergillus flavus. The extent of Aspergillus fungal invasion in maize grains and the resulting increase in aflatoxin levels is heavily influenced by pre-and post-harvest practices. Before harvest, factors such as insect damage and exposure to warm, humid conditions increase the crop's susceptibility to Aspergillus infection and subsequent aflatoxin formation. Post-harvest, additional risks arise when kernels are damaged by pests like rats or when they are stored in unfavorable …