Life Sciences Commons^™

Genomic Epidemiology Of Staphylococcus Aureus Sequence-Type 72, Sachitaa Senthilkumar

Honors Undergraduate Theses

Staphylococcus aureus (S. aureus, SA) is a gram-positive bacterial colonizer and pathogen commonly carried on the skin and in the anterior nares of humans. SA colonization may lead to a variety of non-invasive (e.g., skin and soft tissue infections, SSTIs) and invasive (e.g., bacteremia and osteomyelitis) infections. The SA population is comprised of multiple lineages that can be delineated through multi-locus sequence typing (MLST), which compares nucleotide sequences within seven housekeeping genes. Strains belonging to an MLST share a common evolutionary history and phenotypic characteristics like antibiotic resistance or virulence. As a result, the epidemiology of lineages can be …

Effects Of Changes In Strouhal And Reynolds Numbers On Beetle Wing Aerodynamics, Keith L. Thiha

Honors Undergraduate Theses

Insect flight has long been a subject of interest across the scientific community, largely due to the exceptional aerodynamic performance insects demonstrate relative to their size. Insects tend to have very high aerodynamic performance characteristics like thrust and lift generation compared to their size. This has many potential applications in improving aircraft performance, however the flow phenomena concerning insect wing aerodynamics is still an area of ongoing research. This study aims to investigate how variations in key nondimensional flow parameters—specifically the Reynolds number and Strouhal number—affect the aerodynamic performance of beetle wings. Strouhal numbers ranging from 0.2 to 0.6 were …

The Antibiofilm Efficacy Of Copper And Zinc-Enhanced Borate Bioactive Glasses On Polymicrobial Biofilms, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Healthcare-acquired infections (HAIs) are a significant global challenge driven by biofilm-forming pathogens. Polymicrobial biofilms, involving interactions between multiple microbial species, exacerbate treatment difficulties due to their enhanced resistance to antimicrobial therapies. Borate bioactive glasses (BBGs) are an emerging class of biomaterials that have attracted significant interest in infection control. The incorporation of copper and zinc into the BBG matrix can effectively disrupt biofilm formation and bacterial colonization. This study investigates the antibiofilm efficacy of copper and zinc-doped BBGs against polymicrobial biofilms formed by S. epidermidis, E. coli, and P. aeruginosa. Using static and dynamic biofilm models, BBGs were applied through …

Cold Atmospheric Plasma Induced Degradation Of Organophosphate Pesticides On Kevlar Swatches, Ta Chun Lin, Victor Somtochukwu Mbanugo, Boluwatife Stephen Ojo, Yue-Wern Huang, Marek Locmelis, Frank Daoru Han

Biological Sciences Faculty Research & Creative Works

Cold atmospheric plasma (CAP) was evaluated for degrading organophosphate pesticide (OP) residues (acephate, malathion, and dimethoate) from Kevlar fabrics. Fourier transform-Infrared (FTIR) spectroscopy, scanning electron microscope (SEM) imaging and tensile strength testing confirmed that CAP treatment preserved Kevlar's structural integrity and mechanical properties. Results show degradation efficiency increased with higher power, shorter discharge gaps, and longer exposure duration. Hyperspectral imaging supported the detection of plasma-induced spectral changes, reinforcing the presence of reactive species. The strong oxidative potential of CAP facilitated the rapid breakdown of OPs into nontoxic byproducts. These findings demonstrate the potential of CAP as a portable, energy-efficient solution …

Characterizing The Transient Emission Of Particles And Gases From A Single Puff Of Electronic Cigarette Smoke, Kashala Fabrice Kapiamba, Steven Achterberg, Ta Chun Lin, Philip D. Whitefield, Yue-Wern Huang, Yang Wang

Chemistry Faculty Research & Creative Works

This study employed high-time-resolution systems to examine the transient properties of aerosols and gases emitted from electronic cigarette (EC) puffs. Using a fast aerosol sizer, we measured particle size distributions (PSDs) across various EC brands (JUUL, VUSE, VOOPOO), revealing sizes ranging from 5 to 1000 nm at concentrations of 107 to 1010 cm-3. Most aerosols were found to be in the ultrafine range (below 100 nm), with JUUL-, VUSE-, and VOOPOO-producing aerosols with geometric mean sizes of 19.9, 47.3, and 29.4 nm, respectively. Applying the International Commission on Radiological Protection (ICRP) deposition model and assuming no further evolution of aerosols …

Synthesis And Characterization Of Free Radical Scavenging Dendrimer Nanogels Via Cross-Linking Reaction-Enabled Flash Nanoprecipitation, Lin Qi, Da Huang, Huari Kou, Anna Chernatynskaya, Nuran Ercal, Hu Yang

Chemistry Faculty Research & Creative Works

This work reports the development and evaluation of dendrimer-based nanogels based on Poly amidoamine (PAMAM) dendrimer generation 5, engineered to act as a carrier with reactive oxygen species (ROS)-scavenging capabilities. We developed a cross-linking reaction-enabled flash nanoprecipitation method in which the cross-linking reaction occurs during the flash nanoprecipitation process to form a cross-linked nanostructure. Using this approach, an N-hydroxy succinimide (NHS)-functionalized ROS-responsive thioketal cross-linker (TK-NHS) was synthesized and utilized to cross-link DAB-core PAMAM dendrimer G5, resulting in the formation of G5-TK nanogels. The resulting nanogels were characterized using dynamic light scattering and transmission electron microscopy, and their cytocompatibility, irritancy, cellular …

Pla-Based Bone Tissue Engineering Scaffolds Incorporating Hydroxyapatite And Bioactive Glass Using Digital Light Processing, Engin Gepek, Fateme Fayyazbakhsh, Lev Suliandziga, Vadym Mochalin, Osman Iyibilgin, Yue-Wern Huang, Ming C. Leu

Chemistry Faculty Research & Creative Works

Bone tissue engineering (BTE) aims to repair bone defects using biocompatible materials with tailored geometries and pore structures, providing appropriate mechanical support and control over biodegradation kinetics to promote bone growth. In this study, we utilized digital light processing (DLP) 3D printing to fabricate scaffolds with varying pore sizes using polymer–ceramic slurries composed of polylactic acid (PLA) as the main polymer matrix, incorporated with hydroxyapatite (HA) and bioactive borate glass (BBG) at various ratios. We studied the effect of composition on rheological behavior, printability, mechanical properties, bioactivity, degradation rate, and biocompatibility. While HA increased viscosity and reduced printing accuracy, it …

In The Clear: Timber Harvest Informs Elk Summer Nutrition And Resource Selection In A Densely Forested Landscape, Trevor C. Weeks

Graduate Student Theses, Dissertations, & Professional Papers

As wildlife and habitats face unprecedented challenges in the form of landscape conversion, shifting disturbance regimes, and climate change, it becomes increasingly important for land and wildlife managers to find avenues for collaboration as they seek to maintain habitat to support robust wildlife populations. Timber harvest may provide one such avenue for collaboration. It has been shown to increase the quality and quantity of ungulate nutritional resources by reverting stands to early successional stages. Additionally, timber harvest may allow for a more precise application of such effects, as managers choose where and how to harvest timber. However, the exact effects …

Citrus Gall Wasp Control, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

Citrus gall wasp (Bruchophagus fellis) is an Australian native insect from northern NSW and Queensland and is now established in most Perth suburbs. It affects all citrus species, particularly lemons and grapefruit, and will be an ongoing pest to manage for Perth gardeners, like other common pests. It has not been found in commercial citrus orchards in Western Australia yet.

The Effect Of Parental Behavior On The Ontogeny Of The Immune System In The Eastern Bluebird (Sialia Sialis), Wyatt Boehm, William Kirkpatrick, Sarah E. Durant

2025 Research Poster Competition

Previous research has explored the trade-offs between growth and immune endpoints in offspring since early development is critical in shaping adult responses to disease. However, an unexplored aspect of immune development is the influence of parental behavior on immune outcomes. I observed parental behavior during early development and its influence on offspring immune condition. To analyze parental behavior during incubation in the Eastern Bluebird, I quantified incubation constancy (percentage of time spent on the nest) and its impact on white blood cell ratios throughout development. Incubation constancy is useful in defining general trends in adult incubation behavior. Here, I have …

Cholesterol Modulation Of Bk Currents And Cerebral Artery Diameter Via Channel-Forming Alpha-Subunit, Elizabeth Schneider

Theses and Dissertations (ETD)

Voltage- and calcium-gated, large conductance potassium channels (BK; MaxiK) are ubiquitously expressed and subsequently mediate numerous physiological processes. Cholesterol is consumed in high amounts with the diet and accumulates in cellular membranes when plasma levels become elevated. Cholesterol is a known inhibitor of BK channels and is suspected of contribution to many pathophysiological issues via its interaction with these channels. However, the molecular mechanisms that drive cholesterol inhibition of BK channels remain largely unexplored. This dissertation hypothesized that cholesterol inhibits BK currents via binding to specific amino acid(s) of the channel-forming alpha subunit, and involving distinct modifications of channel gating. …

The Neutral Detergent Fiber And The Acid Detergent Fiber Of The Grass Samples Between Small/Frequent And Large/Infrequent Rainfall Treatments In The C3 And C4 Plant Communities., Zigeng Chen, A. Joshua Leffler

NRM Departmental Data Sets

Data description:
Month: Time of data collection from 2020 to 2022.
Plant community: C3 and C4 plant community.
Treatment: small/frequent (S/F) and large/infrequent (L/I) rainfall treatment.
NDF (g/g DM): The dry matter content of neutral detergent fiber in the grass samples.
ADF(g/g DM): The dry matter content of acid detergent fiber in the grass samples. Cellulose (g/g DM): The dry matter content of cellulose in the grass samples.
Lignin (g/g DM): The dry matter content of lignin in the grass samples.

File type: .csv
File size: 16 KB

Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin

Electronic Theses and Dissertations

The pinyon jay (Gymnorhinus cyanocephalus) is a highly social, colonially nesting corvid endemic to the interior western United States whose populations have declined by ~72% since 1966, prompting the species’ current review for listing under the Endangered Species Act. Pinyon jays inhabit pinyon pine (Pinus subsect. Cembroides) and juniper (Juniperus spp.) woodlands and other dry conifer woodlands, portions of which are treated through tree thinning and removal for fuels reduction, ecological restoration, and habitat improvement for wildlife. As a result there is pressing need to better understand how woodland treatment and other potential drivers impact pinyon jay habitat suitability and …

Machine Learning And Uass For Managing Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mines, Sean Michael Keane

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research introduces an innovative approach for managing autumn olive (Elaeagnus umbellata) on reclaimed surface mines through the integration of machine learning algorithms and unmanned aerial systems. The proliferation of autumn olive presents significant ecological challenges on disturbed landscapes, necessitating more efficient detection and control methods. This study evaluated Random Forest classification and deep learning approaches using the U-Net architecture for identifying autumn olive across four phenological stages using high-resolution imagery. The RGB U-Net model achieved F1-scores of 0.927, 0.912, and 0.871 for autumn olive detection during peak, late, and senescence stages respectively, outperforming both multispectral and vegetation …

Lives And Lessons Of Zoo Animals, Nicole Emlen

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This project was an interdisciplinary museum exhibit that was displayed at the Montana Natural History Center between April 4 and May 12, 2025. It displays my photographs that I took of some of the animals I worked with during my summer 2024 internship at ZooMontana. In this project, I educate the public about three main functions of zoos--rescue, conservation, and education--through the stories and lives of four species. These animals all have unique stories that are important to share to improve public knowledge of why zoos are important and how we can improve relationships between humans and wildlife. Photography is …

Foraging Opportunists: Encouraging Reconnection With The Land, Madelyne Miller, Sylvia Blodorn, Aine Fannon, Ada Scott, Luke Pruitt

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Contextualizing the history of opportunist and native plant species opens options for land management and reconnection to cultural land practices. By recognizing that opportunist species often spread as a result of relocation for practical usage, we determine that reintroducing these uses can lead to an escalation in harvesting these plants. Uses of opportunist plants are diverse and include food sources, artistic practices, and medicine. Foraging also leads to improved human-land-connectedness as well as a deeper connection to ancestral and cultural traditions, which has been found to improve mental health. Focusing on this method of land management will limit the need …

Balancing Timber Harvest And Biodiversity: Impacts Of Selective Logging On Wildlife In The Chiquibul Forest Reserve, Belize, Kilynn Groen

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Selective logging is often promoted as a sustainable forestry practice that maintains biodiversity and ecosystem function, yet its ecological consequences remain under examined, particularly in tropical forests. This study investigates how selective logging affects wildlife communities in the Chiquibul Forest Reserve, Belize. Using remote camera trap data from 22 sites (9 unlogged forest sites and 13 logged (stump) sites), we assessed species richness, trophic structure, and environmental predictors of wildlife abundance. While overall wildlife captures and species richness did not differ significantly between forest and stump habitats, principal component analysis revealed strong community-level separation, with stump habitats supporting distinct species …

Assessing Intra-Seasonal Changes In Summer Elk Diet Composition, Ava E. Window

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Elk (Cervus canadensis) require adequate forage to fulfill their seasonal nutritional requirements. Elk undergo their highest nutritional demands during summer to support critical life functions such as late gestation, lactation, and juvenile growth. However, variations in plant phenology significantly influence the quality and quantity of forage available to elk during the summer season. As summer advances and plant communities mature, forage quality often declines, leading to a landscape that imposes greater nutritional constraints on elk. As a result, prior research on elk foraging habits has primarily focused on late summer. This has led to limited knowledge surrounding the …

Above And Belowground Biomass Of A Multidecadal Restored Tallgrass Prairie Chronosequence And Its Influence On Soil Carbon Dynamics, Adam John Bauer

Graduate Research Theses & Dissertations

Evidence supports the idea that tallgrass prairie restoration has the potential to sequester carbon, but the influences of site age and quality on restored tallgrass prairie carbon dynamics are not well known. This dissertation characterizes a multidecadal tallgrass prairie chronosequence and evaluates the influence of both restoration quality and time since restoration on biomass and soil carbon dynamics using both remotely sensed and field data collected from Nachusa Grasslands tallgrass prairie restorations. It also expands on the traditional aspatial analyses using geographically weighted data to examine the spatiotemporal variation in environmental variables that influence restored tall grass prairie carbon dynamics. …

“Fixing” Settler Capitalism: Un/Sustainability In The Former Fort Ord, Clare M. Beer, Sara Salazar Hughes

Urban and Environmental Studies Faculty Works

California's former Fort Ord Army Base is located on the unceded Indigenous territory of the Ohlone Costanoan Esselen Nation. After seven decades as a training ground for foreign wars, the decommissioning of the base triggered an economic, demographic, and cultural crisis for greater Monterey. The solution to this crisis, the Fort Ord Base Reuse Plan, promised sustainable development in the form of local environmental protection, public higher education, and economic growth. We argue that the Fort Ord Base Reuse Plan illustrates a settler sustainability fix, which the state deploys to secure settler capitalist futurity on the Monterey Peninsula at the …

The Responses Of Insect Herbivores And Local Food Webs To Invasive Shrubs, Logan W. Huber

Browse all Theses and Dissertations

Invasive species cause widespread ecological disruption and economic loss. Invasive plants in particular have the potential to reshape forest food webs from the bottom up. However, the extent to which herbivores colonize and assimilate invasive plants into their diets remains unclear. To address this, I surveyed caterpillar abundance, richness, and herbivory on two invasive shrubs, Lonicera maackii and Elaeagnus umbellata, across regions differing in infestation history. Lonicera maackii supported lower overall caterpillar abundance and richness but hosted a more diverse and abundant caterpillar community in the heavily infested region. E. umbellata supported robust caterpillar communities and experienced herbivory levels comparable …

Evidence That Ultraviolet B Radiation Generates Systemic Responses Via Microvesicle Particle, Niharika Annapureddy

Browse all Theses and Dissertations

Ultraviolet B (UVB) radiation, ranging from 280–315 nm, has been identified as carcinogenic since 1928 and is known to cause skin cancer. Additionally, UVB exposure triggers inflammatory responses, systemic effects, and organ damage. Recent research has discovered that these adverse outcomes are facilitated by the release of microvesicle particles (MVP) via the inflammatory lipid mediator, Platelet-activating Factor (PAF). We have previously demonstrated that sunburn causes skin keratinocytes to release high levels of MVP by activating the PAF receptor (PAFR). These studies were designed to test if these microvesicles enter the bloodstream and travel to various organs, especially the gut, increasing …

Synthetic Nucleoside Labels For Studying Cell-Free Dna, Dibya Guragai

Browse all Theses and Dissertations

Cell-free DNA (cfDNA) can arise from dying cells or be actively secreted by living cells, and is found either unbound, within extracellular vesicles, or in other macromolecular complexes. cfDNA plays roles in intercellular communication and immune responses and is clinically used for diagnostics through PCR and DNA sequencing. Interestingly, our lab has detected lesions in cfDNA after treatment with DNA-damaging agents including UVB and cisplatin. These findings prompted further investigation into which DNA lesions and labels can be found in the cell-free space. Importantly, we sought a mechanism of labeling DNA with a synthetic nucleoside to enable robust tracing using …

Systemic Effects Of Varying Degrees Of Thermal Burn Injuries In Young Mice: Role Of Microvesicle Particles And Platelet-Activating Factor, Prince Wilfred

Browse all Theses and Dissertations

Thermal burn injuries (TBI) result in local and systemic effects, particularly when a large body surface area is exposed to TBI. This study investigates the systemic effects of varying degrees of TBI in the murine model, focusing on the role of microvesicle particles (MVP) and Platelet-activating Factor (PAF) in mediating bacterial translocation and systemic inflammation. Utilizing the murine model, we analyzed MVP levels in the skin and blood, bacterial translocation in the gut, and the expression of pro-inflammatory mediators in the major organs at the 2-hour post-burn time point. Our studies revealed that after the two hours’ time point TBI …

Effects Of Canagliflozin And Pioglitazone On Cardiac Ras And Sirt1 In Db/Db Diabetic Mice, Purab Sanjay Sood

Browse all Theses and Dissertations

Type 2 diabetes significantly increases the risk of diabetic cardiomyopathy (DCM) and nephropathy, largely due to hyperglycemia-induced activation of the renin-angiotensin system (RAS) and elevated levels of angiotensin II (Ang II). Angiotensin converting enzyme 2 (ACE2) and neprilysin (NEP) mitigate the deleterious effects of Ang II by generating Ang-(1-7), a cardioprotective vasodilator. ACE2 is essential for cardiac function, while its deficiency exacerbates dysfunction. Furthermore, the silent information regulator 1 (SIRTl) promotes ACE2 expression and contributes to cardiovascular protection. Despite the availability of several classes of antidiabetic medications, some cardiovascular complications of diabetes persist, and intensive glycemic control does not always …

Enhancing Health And Wellness In Female University Students: A Study On The Impact Of Self-Monitoring, Goal Setting, And Feedback, Halle Landies

Masters Essays

Incorporating exercise into daily routines helps female university students navigate the challenges of university life, promotes long-term health, and fosters a balanced and productive lifestyle (Kohl, 2013). This study aims to expand the research on applied behavior analysis by examining the effectiveness of self-monitoring, goal setting, and feedback in helping female students achieve daily goals related to step count, exercise, distance walked, etc.. Using a changing criterion design, participants recorded data via an Apple Watch, with the intervention's effectiveness evaluated weekly over a six-week period.

A Unionid Mussel Biodiversity Hotspot Experiencing Unexplained Declines: Evaluating The Influence Of Chemical Stressors Using Caged Juveniles, W. Aaron Wilson, Christine Bergeron, Jennifer Archambault, Jason M. Unrine, Jess Jones, Braven Beaty, Damian Shea, Peter R. Lazaro, Jody L. Callihan, Jennifer J. Rogers

Plant and Soil Sciences Faculty Publications

Unionid mussel populations in a section of the Clinch River in Virginia, USA, has declined substantially, but the causes of the decline remain unknown. To investigate this zone of decline (ZOD), we deployed juvenile freshwater mussels (Villosa iris in 2012 and Lampsilis fasciola in 2013) in both cages and silos at sites within the Clinch River System. We analyzed mussel tissues for trace element and organic contaminant concentrations, shells for trace elements, and environmental media (total water, dissolved water, particulate sediment, and bedload sediment) for both inorganic and organic contaminants. We found a few differences between mussels deployed in cages …

Dusp8 Gene Expression Is Increased Upon The Stimulation Of Hek293 Cells, Oanh Vo, Lukas Macha

Student Summer Scholars Manuscripts

Dual specificity phosphatase 8 (DUSP8) is one of the phosphatases that oppose the activity of mitogen-activated protein kinases. Previous mouse studies have demonstrated the functional role of DUSP8 in regulating cardiomyocyte growth. However, it is still unclear how the activity of DUSP8 is regulated, and little is known about the regulation of DUSP8 at the gene level. The goal of this study is to determine whether the DUSP8 gene is immediately transcribed upon MAPK activation in the cells. Here, we found that the expression of the DUSP8 gene was significantly increased after stimulation of cells for 30, 60, 90, and …

Breaking Ground: The Impact Of Construction On Nutrient Loads In An Urban Stream, Brody N. Wilson, Shannon Speir, Alana G. Strauss, Caroline G.T. Anscombe

2025 Research Poster Competition

Degradation of water quality is primarily driven by human activities, such as urbanization. Urban Stream Syndrome (USS) can be characterized by the negative physical, chemical, and biological changes to water quality as a result of urbanization. One key effect of USS is the input of excess nutrients, which can drive unwanted macrophyte and algal growth. New construction is prevalent in urbanizing areas, resulting in the influx of nutrients, sediments, and other materials into stream ecosystems. However, few studies have examined the impacts of active construction on water quality. As such, we do not understand how construction affects nutrient transport downstream. …

Simulation Of Photochemical Effects On Air Quality, Grace Li

2025 Research Poster Competition

Nitrogen dioxide is an atmospheric pollutant with largely anthropogenic sources; it is a combustion side product often produced by automobiles and industrial activity. It has negative effects on the respiratory system. In addition to being a pollutant itself, nitrogen dioxide is also a contributor to tropospheric ozone through photochemical reactions, both of which are EPA criteria air pollutants. Nitrogen dioxide and ozone participate in a net-zero reaction cycle in which the formation of ozone is rate-limited by the photolysis rate of nitrogen dioxide. The destruction of ozone and reformation of nitrogen dioxide is a fast reaction unaffected by photochemistry, therefore …