Life Sciences Commons^™

Variation In Thermal Tolerance Plasticity And The Costs Of Heat Exposure In The Estuarine Sea Hare, Phyllaplysia Taylori, Richelle L. Tanner, Rauri C. K. Bowie, Cynthia Y. Wang-Claypool, Jonathan H. Stillman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Climate change is increasing average temperatures and the frequency and intensity of thermal extremes in coastal marine environments. Organisms in coastal marine habitats are accustomed to environmental fluctuations and possess physiological plasticity that may be advantageous in response to increased occurrence of extremes. To examine whether such plasticity is locally adapted to environmental conditions, we investigated the relationship between genetic diversity and thermal tolerance plasticity in 11 populations of the direct-developing intertidal sea hare, Phyllaplysia taylori, on the western coast of the United States. Using whole-organism metrics of muscle function and metabolic rate and a ddRADseq genomic approach, we …

Associations Of Parental Perceived Health With Child Movement Behaviors Within Two-Parent Households, You Fu, Hayley Almes, Nora Constantino, Doria Schmidt, Ryan D. Burns

International Journal of Physical Activity and Health

This study aimed to examine how perceived parental mental and physical health status was associated with child physical activity, screen time, and sleep. Participants were 6- to 17-year-old minors (N = 20,156; 47.9% female) from the 2022 US National Survey of Children’s Health. Dependent variables were children’s physical activity frequency, screen time, and sleep. Independent variables were parents’ physical and mental health status. Using the Actor-Partner Interdependence Model, two adjusted path models were constructed. One for parent mental health associating with parental physical health, another for parent physical health associating with parental mental health–both further associating with child movement behaviors. …

Let’S Be Cool About It: Anticipating Proper, Whole-Human Cryopreservation And The Necessary Oversight For Adequate Consumer Protection, Peter Lyon

Minnesota Journal of Law, Science & Technology

No abstract provided.

The Spatial Landscape Of Cancer Hallmarks Reveals Patterns Of Tumor Ecological Dynamics And Drug Sensitivity, Mustafa Sibai, Sergi Cervilla, Daniela Grases, Eva Musulen, Rossana Lazcano, Chia-Kuei Mo, Veronica Davalos, Arola Fortian, Adrià Bernat, Margarita Romeo, Collin Tokheim, Jordi Barretina, Alexander J Lazar, Li Ding, Dutreneo Study Investigators, Enrique Grande, Francisco X Real, Manel Esteller, Matthew H Bailey, Eduard Porta-Pardo

Faculty, Staff and Student Publications

Tumors are complex ecosystems of interacting cell types. The concept of cancer hallmarks distills this complexity into underlying principles that govern tumor growth. Here, we explore the spatial distribution of cancer hallmarks across 63 primary untreated tumors from 10 cancer types using spatial transcriptomics. We show that hallmark activity is spatially organized, with the cancer compartment contributing to the activity of seven out of 13 hallmarks, while the tumor microenvironment (TME) contributes to the activity of the rest. Additionally, we discover that genomic distance between tumor subclones correlates with differences in hallmark activity, even leading to clone-hallmark specialization. Finally, we …

Immunological Biomarkers Of Response And Resistance To Treatment With Cabozantinib And Nivolumab In Recurrent Endometrial Cancer, Vladimir Roudko, Diane Marie Del Valle, Emir Radkevich, Geoffrey Kelly, Xie Hui, Manishkumar Patel, Edgar Gonzalez-Kozlova, Kevin Tuballes, Howard Streicher, Swati Atale, Lisa Wang, Benito Czinczin, Seunghee Kim-Schulze, Ignacio I Wistuba, Cara L Haymaker, Gheath Al-Atrash, Ganiraju Manyam, Jianjun Zhang, Ryan Thompson, Mayte Suarez-Farinas, Stephanie Lheureux, Sacha Gnjatic

Faculty, Staff and Student Publications

Background: Antiangiogenics combined with immune checkpoint blockade have become standard of care for recurrent endometrial cancer after standard platinum-based chemotherapy. To dissect mechanisms and define biomarkers associated with clinical outcomes to these combinations, we applied multidimensional immune monitoring to peripheral blood specimens collected from a randomized phase 2 trial of nivolumab with or without cabozantinib in 75 evaluable patients with recurrent endometrial cancer (NCI ETCTN 10104, NCT03367741). This trial demonstrated superiority of the combination to nivolumab alone.

Methods and results: Using Olink proteomics, mass cytometry, tumor antigen-specific ELISA, and whole exome tumor sequencing, we identified longitudinal immune signatures specific …

Impact Of Measurable Residual Disease Clearance Kinetics In Patients With Aml Undergoing Intensive Chemotherapy, Wei-Ying Jen, Koji Sasaki, Farhad Ravandi, Tapan M Kadia, Sa A Wang, Wei Wang, Sanam Loghavi, Naval G Daver, Courtney D Dinardo, Ghayas C Issa, Hussein A Abbas, Cedric Nasnas, Alex Bataller, Samuel Urrutia, Omer S Karrar, Sherry Pierce, Hagop M Kantarjian, Nicholas J Short

Faculty, Staff and Student Publications

The prognostic impact of measurable residual disease (MRD) in acute myeloid leukemia (AML) is unequivocal; however, the optimal time point for achieving undetectable MRD is unclear. We retrospectively studied patients with newly diagnosed (ND) AML who achieved remission with frontline intensive chemotherapy and had MRD assessed by flow cytometry after induction (time point 1 [TP1]) and after cycles 2 or 3 (TP2). Cases were grouped into MRD negative (Neg)/Neg, positive (Pos)/Neg, or Pos/Pos at TP1 and TP2, respectively. Of 1980 patients with ND AML, 277 met the inclusion criteria and were included in this analysis. The median relapse-free survival (RFS) …

Joint Angular Kinematics And Gross Motor Function In Typically Developing Healthy Children, Monday Omoniyi Moses, Ngozi Florence Onuegbu, Prince De-Gualle Deku, Mary Abena Nyarko, Lydia Boampong Owusu, Abigael Omowumi Emikpe, Emmanuel Babatunde John, Rahul Soangra, Abiboye Cheduko Yifieyeh, Nicholas Akinwale Titiloye

Physical Therapy Faculty Articles and Research

Objective: The aim of this study was to establish the interactions between joint angular kinematics and gross motor function in typically developing healthy Ghanaian children. Methods: A descriptive cross-sectional study design was employed. A total of 150 (69 (46.0%), 3.25 ± 0.08-year-old boys and 81 (54.0%), 3.25 ± 0.06-year-old girls) 2–4-year-old children were recruited. Joint angular kinematic variables [left hip flexion (LHF), left hip extension (LHE), right hip flexion (RHF), left knee flexion (LKF), right hip extension (RHE), left knee extension (LKE), right knee flexion (RKF), left ankle dorsi-flexion (LADF), right knee extension (RKE), right ankle plantar flexion (RAPF), left …

Pskh1 Kinase Activity Is Differentially Modulated Via Allosteric Binding Of Ca2+ Sensor Proteins, Christopher R Horne, Toby A Dite, Samuel N Young, Lucy J Mather, Laura F Dagley, Jared L Johnson, Tomer M Yaron-Barir, Emily M Huntsman, Leonard A Daly, Dominic P Byrne, Antonia L Cadell, Boaz H Ng, Jumana Yousef, Dylan H Multari, Lianju Shen, Luke M Mcaloon, Gerard Manning, Mark A Febbraio, Anthony R Means, Lewis C Cantley, Maria C Tanzer, David R Croucher, Claire E Eyers, Patrick A Eyers, John W Scott, James M Murphy

Children’s Nutrition Research Center Staff Publications

Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed in prostate, lung, and kidney cancers. However, little is known about PSKH1 regulatory mechanisms, leading to its classification as a “dark” kinase. Here, we used biochemistry and mass spectrometry to define PSKH1’s consensus substrate motif, protein interactors, and how interactors, including Ca2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of a canonical Calmodulin binding motif, Ca2+-Calmodulin activated PSKH1 while, in contrast, the ER-resident Ca2+ sensor of the Cab45, Reticulocalbin, Erc55, Calumenin (CREC) family, Reticulocalbin-3, suppressed PSKH1 catalytic activity. In …

Monitoring And Modeling Legacy Tree Response To Restoration Treatments In The Klamath Mountains, Oregon, Usa, Isaac Joseph Ball

Dissertations and Theses

Climate change and its disruption of historical drought and wildfire patterns pose a substantial threat to forest health in the western United States. This threat is compounded by past management practices like fire suppression. The largest, oldest, and most ecologically valuable trees are particularly vulnerable, making their conservation a key objective of climate-adaptive forest management. Restoration treatments such as mechanical thinning have been shown to enhance tree growth and drought resilience in dry conifer forests, but long-term monitoring is necessary to understand their sustained effects. This study used dendroecological tree-ring analysis to investigate the effects of thinning on the growth, …

Consumption Habitats Of Male And Female Lionfish Regarding The Production Of Eggs, Zalyn Baker, Natalie Tipsword, Sam Fisher

Symposium of Student Scholars

The lionfish, scientifically known as Pterois, belongs to the Scorpaenidae family, which is native to the Indo-Pacific Ocean, but has become an invasive species in the Caribbean Sea. First observed in Belize in 2008, lionfish have since become established in the Belize Barrier Reefs. Known for their ability to consume a wide range of species, lionfish compete with native fish for food resources. This invasive species poses a significant threat to the ecosystem due to their venomous spines, aggressive predatory behavior, lack of predators, and high reproductive rate, with lifespans extending up to 30 years. Our study aimed to investigate …

Microbial Source Tracking Protocols For Differentiation Of Fecal Bacteria From Cow, Deer, And Horse, Nika Shams, Michael Beach

Symposium of Student Scholars

Microbial source tracking (MST) attempts to identify specific animal sources of fecal contamination in the environment. While traditional fecal coliform enumeration can give a general measure of contamination, the specificity imparted by modern MST provides a more informative assessment of contaminants and their likelihood to affect human health outcomes.

We have been developing an MST system that detects DNA biomarkers from the gram-negative bacterial genus Bacteroides and the family Lachnospiraceae. They are strict anaerobes found in large quantities in the intestines of animals. Unlike E. coli or Enterococci, host-specific strains of Bacteroides and Lachnospiraceae exist. To this end, we …

Transcription Factor Binding And Discovery Of Novel Gene In The Opportunistic Human Pathogen, Pseudomonas Aeruginosa, Alaina Westee

Symposium of Student Scholars

Pseudomonas aeruginosa (PA) is a gram-negative, ubiquitously-found bacterium that primarily causes nosocomial, or hospital-borne, infections within immunocompromised patients. Therefore, it has been deemed a critical priority pathogen by the World Health Organization (WHO) and the Center for Disease Control (CDC), generating a need for research on its fundamental biology. The Van Dyke laboratory focuses on proteins called transcription factors, which promote or repress gene expression to regulate an organism’s functioning. We are specifically studying the cadmium-responsive transcription factor, CadR, due to the importance of metals in virulence and the lack of information on PA’s response to metal fluctuations. We intend …

Bloom: Behavioral Learning And Outcome Observation In Microbes, Sean Sarwar Haque, Luke Compton Wharton, Ming Lin, Razvan Voicu

Symposium of Student Scholars

Understanding how pathogens respond to physical changes in their environment is crucial for developing effective treatments and preventative measures. Current research often relies on static models or experimental data that either fail to capture the dynamic interactions within cellular environments or are not generalizable to other types of pathogens. This project aims to address this gap by creating a comprehensive cell simulation that models pathogens and their response to chemical, physical, and physiological changes. The proposed solution is a simulation that integrates biological data and computational modeling to replicate the behavior of pathogens in real time as they are affected …

Survey Of Mosquito Larval Habitats At The Ksu Field Station, Rose Sofia, Eli Lovvorn, Aspen Hartman, Andrew Haddow

Symposium of Student Scholars

The greater Atlanta metropolitan area hosts numerous mosquito species that are nuisance biters and can transmit arthropod-borne diseases to humans. We surveyed the Kennesaw State University (KSU) Field Station – an incredibly biodiverse location – where student, staff, and faculty-led groups engage in outdoor research and teaching activities. The KSU Field Station located in Cobb County, GA is a heavily trafficked 25-acre property that may attract various mosquito species capable of transmitting pathogens to humans and animals alike. On August 27th, 2024, our team investigated the KSU Field Station to quantify and record potential water-holding containers that serve as mosquito …

Gubic: The Global Urban Biological Invasions Compendium For Plants, Daijiang Li, Luke J. Potgieter, Myla F. J. Aronson, Irena Axmanová, Benjamin Baiser, Marta Carboni, Laura Celesti-Grapow, Sonja Knapp, Ingolf Kühn, Ana Carolina Lacerda De Matos, Zdeňka Lososová, Flavia A. Montaño-Centellas, Petr Pyšek, David M. Richardson, Lauren B. Trotta, Rafael D. Zenni, Sarel S. Cilliers, Bruce D. Clarkson, Amy J. S. Davis, Rebecca W. Dolan, Marcin M. Dyderski, Franz Essl, Orou G. Gaoue, Joanne Gui, Charly Géron, Gustavo Heringer, Cang Hui, Anzar A. Khuroo, Stefan Klotz, Peter M. Kotanen, Holger Kreft, Frank A. Sorte, Jonas J. Lembrechts, Bernd Lenzner, Christopher A. Lepczyk, Scott Macivor, Cristina Martínez-Garza, Akira S. Mori, Charles Nilon, Jan Pergl, Stefan J. Siebert, Alyona S. Tretyakova, Toby P. N. Tsang, Kei Uchida, Mark Van Kleunen, Montserrat Vilà, Hua-Feng Wang, Patrick Weigelt, Peter Werner, Nicholas S. G. Williams, Marten Winter, Marc Cadotte

Scholarship and Professional Work - LAS

Abstract Urban areas are foci for the introduction of non-native plant species, and they often act as launching sites for invasions into the wider environment. Although interest in biological invasions in urban areas is growing rapidly, and the extent and complexity of problems associated with invasions in these systems have increased, data on the composition and numbers of non-native plants in urbanized areas remain scattered and idiosyncratic. We assembled data from multiple sources to create the Global Urban Biological Invasions Compendium (GUBIC) for vascular plants representing 553 urban centres from 61 countries across every continent except Antarctica. The GUBIC repository …

The Impact Of Diabetes On Neurovascular Integrity: A Special Focus On Pericytes And Endothelin-1, Mia Edgerton

MUSC Theses and Dissertations

Diabetes increases the risk of stroke and Vascular Contributions to Cognitive Impairment and Dementia (VCID). Diabetes also dysregulates the endothelin (ET) system. ET-1-mediated constriction of brain microvascular pericytes (BMVPCs) has been shown to contribute to brain hypoperfusion. However, there is a loss of BMVPCs in VCID, but underlying mechanisms of pericyte degeneration are poorly studied. ETA receptor activation can instigate phenotypical changes and cellular senescence in endothelial cells. . There is also emerging evidence that ETB receptor agonism improves outcomes in patients with cerebral ischemic stroke, but long-term effects, especially in diabetes, are unknown. Therefore, this study aimed to determine …

What To Do Now That You've Trapped A Nuisance Wild Animal, Cory Farnsworth, S. Nicole Frey

All Current Publications

This fact sheet outlines specific guidelines for legally responding to nuisance wild animals. Management best practices, animal use, euthanasia, and proper disposal are discussed.

Reviews And Syntheses: Variable Inundation Across Earth’S Terrestrial Ecosystems, James Stegen, Amy J. Burgin, Michelle H. Busch, Joshua B. Fisher, Joshua Ladau, Jenna Abrahamson, Lauren Kinsman-Costello, Li Li, Xingyuan Chen, Thibault Datry, Nate Mcdowell, Corianne Tatariw, Anna Braswell, Jillian M. Deines, Julia A. Guimond, Peter Regler, Kenton Rod, Edward K. P. Bam, Etienne Fluet-Chouinard, Inke Forbrich, Kristin L. Jaeger, Teri O'Meara, Tim Scheibe, Erin Seybold, Jon N. Sweetman, Jianqiu Zheng, Daniel C. Allen, Elizabeth Herndon, Beth A. Middleton, Scott Painter, Kevin Roche, Julianne Scamardo, Ross Vander Vorste, Kristin Boye, Ellen Wohl, Margaret Zimmer, Kelly Hondula, Maggi Laan, Anna Marshall, Kaizad F. Patel

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The structure, function, and dynamics of Earth's terrestrial ecosystems are profoundly influenced by how often (frequency) and how long (duration) they are inundated with water. A diverse array of natural and human-engineered systems experience temporally variable inundation whereby they fluctuate between inundated and non-inundated states. Variable inundation spans extreme events to predictable sub-daily cycles. Variably inundated ecosystems (VIEs) include hillslopes, non-perennial streams, wetlands, floodplains, temporary ponds, tidal systems, storm-impacted coastal zones, and human-engineered systems. VIEs are diverse in terms of inundation regimes, water chemistry and flow velocity, soil and sediment properties, vegetation, and many other properties. The spatial and temporal …

02.24.2025 Ored Connect, Liz Williamson

ORED Newsletter

Collaborate Opportunity

SEC Faculty Travel Grants

Participant Support Cost Resources

Regulating Chemoresistance And Cancer Stemness: The Cdh17-Yap Pathway In Distinct Cellular States Of Lung Cancer Ctc Clusters, Zujun Que, Dan Qi, Yun Yang, Wang Yao, Jiajun Liu, Yan Li, Yuanyuan Yu, Luyao Wang, Fangfei Li, Ge Zhang, Erxi Wu, Jianhui Tian

Children’s Nutrition Research Center Staff Publications

Background: Drug resistance in metastatic lung cancer significantly contributes to patient mortality. This study explores the role of circulating tumor cells (CTCs), the precursors to metastasis, in driving this resistance. We aim to delineate the unique biological traits of CTC clusters in lung cancer and elucidate the mechanisms underlying their resistance to chemotherapy.

Methods: We used an ultralow adsorption plate to establish a CTC suspension culture system. Comparisons between adherent and suspension cultures of CTC-TJH-01 cells were made via Cell Counting Kit-8 (CCK-8), western blot, immunofluorescence, and flow cytometry assays to evaluate cell proliferation, drug resistance, and cancer stemness. The …

Managing Lupin Sclerotinia: Know Your Risk And How To Respond, Ciara Beard, Geoff J. Thomas, Zia Hoque, Sarita Bennett, Pippa J. Michael, Rachael Crockett, Shannah Kanny, Montana Bradley

Grain and other field crops research reports

• Sclerotinia prevalence, particularly basal Sclerotinia (ground level infection) has increased significantly in Western Australia in the last five years. Since 2020, commercial crop surveys have found basal Sclerotinia more common in lupins than in canola, and it is difficult to manage.
• From 2021–2025 extensive research was conducted in the laboratory, glasshouse and field trials by research partners Department of Primary Industries and Regional Development (DPIRD), Centre for Crop Disease Management (CCDM), Mingenew Irwin Group (MIG) and lupin growers. Key new findings applicable to lupin growers are:
• Sclerotinia infects narrow-leaf lupin via two pathways: canopy and basal. Basal infection is more …

Determining The Optimal Antioxidant Strategy: A Comparative Analysis Of Extractionmethods In Cistus Villosus L. Extracts Revealed By Lc-Hrms And Computationalmodeling, Hicham Bouakkaz, Ebru Erol, Khedidja Benarous, Gülaçti Topçu, Reguia Mahfoudi, Amar Djeridane, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Cistus villosus L., a beloved tea herb, contains a treasure trove of potent antioxidants. Our research unveils thisancient ally’s potential to shield against oxidative stress, a modern health threat.Materials and methods: Antioxidant assays were conducted to evaluate the potency of ethyl acetate extract. IC50 values were determinedfor the extract in various antioxidant assays. Furthermore, molecular docking was employed for the examination of the 22 compoundsanalyzed through LC-HRMS. Additionally, ADMET and PASS investigations were conducted to evaluate their capability as antioxidantsand inhibitors of oxidant formation.Results: Each identified compound boasts a history of antioxidant prowess, building the natural defense arsenal of …

Enhanced Formulation And Comprehensive Analysis Of Novel Natural Ointments Withgrape Seed And Pomegranate Peel Infused In Olive Oil, Mebarka Imane Benguechoua, Madjda Benguechoua, Khedidja Benarous, Alaeddine Kaouka, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: The global shift toward sustainable and organic alternatives in cosmetics and personal care has created a demandfor innovative and environmentally conscious formulations. This study aimed to develop novel natural ointments using beeswax andgrape seed (GS) and pomegranate peel (PP) extracts infused in extra virgin olive oil (EVOO), and determine the physicochemicalcharacteristics, microbiological properties, and antioxidant activity as a way to valorize organic waste toward its application in thecosmetic industry. The research aimed to promote sustainability and effectiveness in the cosmetic industry.Materials and methods: Microbial analysis was performed on the oils and ointments, followed by the extraction of active compoundsusing …

The Therapeutic Potential Of Salvia Aegyptiaca Extracts In Ethanol-Induced Gastric Ulcer:Insights Into Macroscopic, Histopathological, And Biochemical Mechanisms, Walid Mamache, Smain Amira, Fatima Benchikh, Hassiba Benabdallah, Amor Bencheikh, Hind Amira, Roumaissa Ounis, Mohammed Abdallah Torki, Chahrazed Kaoudoune

Turkish Journal of Biology

Background/aim: This study explores the antiulcer activity of different doses of Salvia aegyptiaca (SAE) methanol (ME) and decoctedextracts (DE) on ethanol-induced gastric ulcers in rats.Materials and methods: Female Wistar rats weighing 180–200 g were divided into five groups: control, omeprazole (positive control),and extract-treated (100, 200, and 400 mg/kg). Ulcers were induced with absolute ethanol 30 min after treatment with the extracts.The experiment was followed by macroscopic and histopathological examination. In vitro tests were also conducted to assess lipidperoxidation, catalase activity, mucus content, glutathione, and protein levels.Results: The study found that 100% ethanol caused significant damage, including colour and mucus loss, …

Phytochemical Profile And Nematicidal Potential Of Essential Oil From Algerian Wildoriganum Vulgare Subsp. Glandulosum Defs., Amina Mezerket, Juan Emilio Palomares Rius, Souad Bouasla, Henia Saib

Turkish Journal of Biology

Background/aim: The root gall nematode Meloidogyne incognita constitute the most damaging species that infects many crops inAlgeria. The intense use of harmful agricultural chemical products has incited research to develop alternative methods with natural andecological advantages like essential oils extracted from plants. The objective of this study was to evaluate the efficacy of Origanum vulgaresubsp. glandulosum Desf. (Lamiaceae) essential oil on the development of the root-knot nematode M. incognita in potted tomatoes.Materials and methods: In pot trials, we assess the activity of O. vulgare subsp. glandulosum essential oil at two concentrations of0.75 and 0.37 mg /L against M . incognita. …

Antidiabetic Potential Of Polysaccharides From Algerian Saharan Zygophyllum Geslini Instreptozotocin-Induced Diabetic Rats, Houria Medjdoub, Waffa Bouali, Arezki Azzi, Nacera Belkacem, Nabila Benariba, Nawel Meliani

Turkish Journal of Biology

Background/aim: Zygophyllum geslini, an endemic Algerian species, has numerous properties, especially as an antidiabeticdrug. In Algeria, this herb serves as condiment in Saharan dishes and as animal feed (Sheep, Goat and Camel). However, fewscientific studies have reported on the medicinal properties of this Saharan species. The aims of the present work were to study 1) thechemical aspects of polysaccharides extracted from this plant, 2) their inhibitory effect on pancreatic α-amylase in vitro, and 3) theirantihyperglycemic effect in streptozotocin-induced diabetic rats in vivo.Materials and methods: First, polysaccharides were extracted from Z. geslini aerial part (ZGAP) in hot water and precipitated withethanol …

Antilipase Activities Of Cultivated Peppermint And Rosemary Essential Oils:In Vitro And In Silico Studies, Khadidja Bengana, Talia Serseg, Khedidja Benarous, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka

Turkish Journal of Biology

Background/aim: The growing interest in essential oils clearly indicates the power of nature and aligns with our increasing need to findtherapeutic solutions in the natural world. This study aimed to investigate the inhibitory effects of the essential oils of Mentha × piperitaand Salvia rosmarinus, harvested from the Laghouat region of Algeria, against Candida rugosa lipase (CRL) and pancreatic lipasethrough both in vitro and in silico studies.Materials and methods: Essential oils were extracted via hydrodistillation and analyzed using gas chromatography–mass spectrometryand spectrophotometry. Their antilipase activities were assessed using an inhibition assay, and molecular docking was performed withAutoDock Vina to explore interactions …

Investigating The Composition Of Fatty Acid, Tocopherol, And Sterol Contents Andantioxidant Capacity Of Oils From The Seeds Of Abelmoschus Esculentus L. Growing Inalgeria, Samira Nia, Madjda Benguechoua, Toufik Hadj Mahammed, Khatir Reggagui, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Vegetable oils are considered a vital source of energy when glucose cannot be utilized. Therefore, research on newsources of oil that demonstrate nutritional value is necessary. Okra (Abelmoschus esculentus), a member of the Malvaceae family, isa remarkable botanical specimen due to its various uses; its roots, stem, leaves, fruits, and seeds are all valued for their nutritional,therapeutic, and industrial value. This study aims to demonstrate okra’s fatty acid (FA) composition, tocopherol content, and sterolcontent and evaluate the antioxidant activity of the oils extracted from okra using three solvents.Materials and methods: The initial part of this study involved oil extraction …

Inhibitory Effects Of Carvacrol On Glucansucrase From Streptococcus Mutans And Salivaryα-Amylase: In Silico And In Vitro Studies, Samet Kocabay, M. Abdullah Alagöz, Bi̇rnur Akkaya

Turkish Journal of Biology

Background/aim: Streptococcus mutans produces glucansucrase, an enzyme that converts sucrose into lactic acid, which lowers the pH in the oral environment and leads to tooth enamel demineralization, a key factor in dental caries. Additionally, glucansucrase facilitates the formation of extracellular polysaccharides, which promote bacterial adhesion to tooth surfaces. This study investigates the inhibitory effects of carvacrol, a natural compound, on glucansucrase activity both in vitro and in silico. Materials and methods: Glucansucrase enzyme was purified from S. mutans. The inhibitory effects of carvacrol against glucansucrase enzyme were investigated both in vitro and in silico. Results: In the presence of 50 …

Evaluating The Multifaceted Bioactivity Of Syzygium Aromaticum Essential Oil:The Central Role Of Eugenol, Noureddine Rahim, Chamseddine Derabli, Amina Bramki, Sara Mahdjoub, Sandrine Rup-Jacques, Ghozlane Barboucha, Stephani Hesse, Houssem Boulebd

Turkish Journal of Biology

Background/aim: Syzygium aromaticum L. is a versatile plant traditionally used to treat digestive and respiratory issues, improve oral health, and relieve pain, particularly in regions such as Southeast Asia, South Asia, and parts of the Middle East. Its essential oil (EO), predominantly composed of eugenol, is rich in bioactive compounds. This study aims to clarify the specific contribution of eugenol to the antioxidant, antibacterial, antifungal, and insecticidal activities of S. aromaticum EO by comparing their individual effects. Additionally, density functional theory (DFT) calculations were employed to examine the antioxidant mechanism of eugenol and its role in enhancing the overall activity …